MC08g1343 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1343 vs. ExPASy Swiss-Prot
Match: Q9LFR0 (Alpha-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GMII PE=1 SV=1)

HSP 1 Score: 1654.0 bits (4282), Expect = 0.0e+00
Identity = 817/1176 (69.47%), Postives = 941/1176 (80.02%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRR-------GGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANF 60
            M FSS+   G+ RR       GGW  S+LP  T    +K    RK R+R  V +FIFANF
Sbjct: 1    MPFSSYI--GNSRRSSTGGGTGGWGQSLLP--TALSKSKLAINRKPRKRTLVVNFIFANF 60

Query: 61   FTIGFLISLIFFFIVLLRYGVPKPISSHF-RSHATRSHRPRRPIVSENWNREVLSANVDI 120
            F I   +SL+FF + L  +GVP PISS F  S + R  +PR+ I     N     A VDI
Sbjct: 61   FVIALTVSLLFFLLTLFHFGVPGPISSRFLTSRSNRIVKPRKNINRRPLNDSNSGAVVDI 120

Query: 121  TTKELYDKIEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYE 180
            TTK+LYD+IEFLD DGGPWKQGW+VTY  DEW+ EKLK+FVVPHSHNDPGWKLTV+EYY+
Sbjct: 121  TTKDLYDRIEFLDTDGGPWKQGWRVTYKDDEWEKEKLKIFVVPHSHNDPGWKLTVEEYYQ 180

Query: 181  RQSRRILDTIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGG 240
            RQSR ILDTIVE LSKDSRRKFIWEEMSYLE+WWRDAS  K+EA   LVK+GQLEIVGGG
Sbjct: 181  RQSRHILDTIVETLSKDSRRKFIWEEMSYLERWWRDASPNKQEALTKLVKDGQLEIVGGG 240

Query: 241  WVMNDE-----------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENM 300
            WVMNDE           +AEGNMWLN+TIGV+PKNSWAIDPFGYS TMAYLLRRMGFENM
Sbjct: 241  WVMNDEANSHYFAIIEQIAEGNMWLNDTIGVIPKNSWAIDPFGYSSTMAYLLRRMGFENM 300

Query: 301  LIQRTHYELKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQ 360
            LIQRTHYELKK+LA HKN+E+ WRQSWDA ETTDIFVHMMPFYSYDIPHTCGPEPAICCQ
Sbjct: 301  LIQRTHYELKKDLAQHKNLEYIWRQSWDAMETTDIFVHMMPFYSYDIPHTCGPEPAICCQ 360

Query: 361  FDFARSRGSIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFR 420
            FDFAR RG  YELCPW   PVE   ENVQERA+ LLDQYRKKS LYRTNTLLIPLGDDFR
Sbjct: 361  FDFARMRGFKYELCPWGKHPVETTLENVQERALKLLDQYRKKSTLYRTNTLLIPLGDDFR 420

Query: 421  YITIDEAEAQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGS 480
            YI+IDEAEAQF+NYQ+LFDHINSNPSLNAEAKFGTLEDYFRT+R+EA+++NYS PGEVGS
Sbjct: 421  YISIDEAEAQFRNYQMLFDHINSNPSLNAEAKFGTLEDYFRTVREEADRVNYSRPGEVGS 480

Query: 481  SQISGFPSLSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQ 540
             Q+ GFPSLSGDFFTY+DRQQDYWSGYYVSRPFFKAVDRVLE TLR AE+M++ LLG+C 
Sbjct: 481  GQVVGFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEHTLRGAEIMMSFLLGYCH 540

Query: 541  RSQCEKFPIGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSK 600
            R QCEKFP  F+YKLTAARRNLALFQHHDGVTGTAKD+VV+DYG RMHTSLQDL IFMSK
Sbjct: 541  RIQCEKFPTSFTYKLTAARRNLALFQHHDGVTGTAKDYVVQDYGTRMHTSLQDLQIFMSK 600

Query: 601  GIEVLLGIRH--EKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTR 660
             IEVLLGIRH  EKSD +   FE EQMRSKYD +PVH+ I  +  +  +V+ FNP EQTR
Sbjct: 601  AIEVLLGIRHEKEKSDQSPSFFEAEQMRSKYDARPVHKPIAAREGNSHTVILFNPSEQTR 660

Query: 661  EEVVMIIVNMTDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYY 720
            EEVV ++VN  ++SVLDSNWTC+ SQ+SPE QHD  K FTGRHRL+WK S+PALGL+TY+
Sbjct: 661  EEVVTVVVNRAEISVLDSNWTCVPSQISPEVQHDDTKLFTGRHRLYWKASIPALGLRTYF 720

Query: 721  IANGLFGCEKPKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQ 780
            IANG   CEK  P+KL + S+    PCP PY CSK D DV EI+N+HQ+LVFDV +G L+
Sbjct: 721  IANGNVECEKATPSKLKYASEFDPFPCPPPYSCSKLDNDVTEIRNEHQTLVFDVKNGSLR 780

Query: 781  KVIKKSGSQNVVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYP 840
            K++ ++GS+ VV EEI MYSS  SGAYLF P G+A+ I++  G  V  EG L+QEV+SYP
Sbjct: 781  KIVHRNGSETVVGEEIGMYSSPESGAYLFKPDGEAQPIVQPDGHVVTSEGLLVQEVFSYP 840

Query: 841  KTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYY 900
            KT+WEKSP+S  TRLY+G + +Q  ++E EYHVELLG +FDDRELIVRYKTDVDNK+++Y
Sbjct: 841  KTKWEKSPLSQKTRLYTGGNTLQDQVVEIEYHVELLGNDFDDRELIVRYKTDVDNKKVFY 900

Query: 901  SDLNGFQMSRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEI 960
            SDLNGFQMSRRE+YDKIPLQGNYYPMPSLAF++GSNGQRFS+HS+QSLGVASLK+GWLEI
Sbjct: 901  SDLNGFQMSRRETYDKIPLQGNYYPMPSLAFIQGSNGQRFSVHSRQSLGVASLKEGWLEI 960

Query: 961  MLDRRLTRDDGRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGA 1020
            MLDRRL RDDGRGLGQGV DNR M VVFH L ESN+ S+ +P  N +  + SLLSH +GA
Sbjct: 961  MLDRRLVRDDGRGLGQGVMDNRAMTVVFHLLAESNI-SQADPASNTNPRNPSLLSHLIGA 1020

Query: 1021 RLNYPLHTFIAKKPQPSSVQPPP-RSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPED-P 1080
             LNYP++TFIAKKPQ  SV+ P   SF+PLA PLPCDLHIV+FKVPRP KYSQQ  ED P
Sbjct: 1021 HLNYPINTFIAKKPQDISVRVPQYGSFAPLAKPLPCDLHIVNFKVPRPSKYSQQLEEDKP 1080

Query: 1081 RFILIFHRRHWDSSYCKTARS-NCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEM 1140
            RF LI +RR WDS+YC   R  NCT  A+EP N  +MFK    S  + TSLNLL ED E+
Sbjct: 1081 RFALILNRRAWDSAYCHKGRQVNCTSMANEPVNFSDMFKDLAASKVKPTSLNLLQEDMEI 1140

Query: 1141 LGYNEQ----SGDVAHEGQLHIPPMELRAYKLEVQP 1149
            LGY++Q          EG++ I PME+RAYKLE++P
Sbjct: 1141 LGYDDQELPRDSSQPREGRVSISPMEIRAYKLELRP 1171

BLAST of MC08g1343 vs. ExPASy Swiss-Prot
Match: P49641 (Alpha-mannosidase 2x OS=Homo sapiens OX=9606 GN=MAN2A2 PE=2 SV=3)

HSP 1 Score: 728.8 bits (1880), Expect = 9.6e-209
Identity = 425/1054 (40.32%), Postives = 613/1054 (58.16%), Query Frame = 0

Query: 119  DKIEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRI 178
            +++ F +VDGG W+QG+ ++Y+  +WD+E L+VFVVPHSHNDPGW  T D+YY  Q++ I
Sbjct: 136  EELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHI 195

Query: 179  LDTIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE 238
            L+++V  L +D RR+F+W E+S+  KWW + + +KR A   LV NGQLEI  GGWVM DE
Sbjct: 196  LNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDE 255

Query: 239  -----------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTH 298
                       + EG+ WL   +G  P++ WA+DPFGYS TM YLLRR    +MLIQR H
Sbjct: 256  ANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVH 315

Query: 299  YELKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARS 358
            Y +KK  A   ++EF WRQ+WD++ +TDIF HMMPFYSYD+PHTCGP+P ICCQFDF R 
Sbjct: 316  YAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRL 375

Query: 359  RGSIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDE 418
             G     CPW++ P  I   NV ERA +LLDQYRKKS L+R+N LL+PLGDDFRY    E
Sbjct: 376  PGGRIN-CPWKVPPRAITEANVAERAALLLDQYRKKSQLFRSNVLLVPLGDDFRYDKPQE 435

Query: 419  AEAQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGF 478
             +AQF NYQ LFD  NS P+L+ +A+FGTL DYF  L        Y   G    ++  GF
Sbjct: 436  WDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDAL--------YKRTGVEPGARPPGF 495

Query: 479  PSLSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRS-QCE 538
            P LSGDFF+Y+DR+  YW+GYY SRPF+K++DRVLE  LR AE++ +L   H +RS    
Sbjct: 496  PVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAG 555

Query: 539  KFPIGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDL-HIFMSKGIEV 598
            ++P+     LT ARR L LFQHHD +TGTAK+ VV DYG+R+  SL +L  + +     +
Sbjct: 556  RYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYL 615

Query: 599  LLGIRHE-KSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVM 658
            +LG +     D  +   + +  R  +D  P    I L   S + VV FNPLEQ R  +V 
Sbjct: 616  VLGDKETYHFDPEAPFLQVDDTRLSHDALPERTVIQLD-SSPRFVVLFNPLEQERFSMVS 675

Query: 659  IIVNMTDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGL 718
            ++VN   V VL      L  Q+S  +     +A    +++   + +PALGL    +  GL
Sbjct: 676  LLVNSPRVRVLSEEGQPLAVQISAHWS-SATEAVPDVYQVSVPVRLPALGLGVLQLQLGL 735

Query: 719  FGCEK-PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAE--IQNQHQSLVFDVNHGLLQKV 778
             G    P   ++    +  S+     +     D   ++  + N++  + F    GLL+ +
Sbjct: 736  DGHRTLPSSVRIYLHGRQLSVSRHEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSI 795

Query: 779  IK-KSGSQNVVNEEIAMY----SSLGSGAYLFNPIGDAKSII-EAGGLTVIVEGPLMQEV 838
             +     +  V+ ++ +Y    S   SGAYLF P G+AK  + +   +  + EGP   EV
Sbjct: 796  RRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKPYVPKEPPVLRVTEGPFFSEV 855

Query: 839  YSYPKTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNK 898
             +Y         I  + RLY+    ++G  ++    V++  +++ ++EL +   TD+D++
Sbjct: 856  VAY------YEHIHQAVRLYNLPG-VEGLSLDISSLVDI--RDYVNKELALHIHTDIDSQ 915

Query: 899  RIYYSDLNGFQMSRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDG 958
             I+++DLNGFQ+  R    K+PLQ N+YPMP +A+++ +  +R ++H+ Q+LGV+SLKDG
Sbjct: 916  GIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQ-KRLTLHTAQALGVSSLKDG 975

Query: 959  WLEIMLDRRLTRDDGRGLGQGVTDNRVMNVVFHTLIE-SNVSSKLNPVPNHSSLSASLLS 1018
             LE++LDRRL +DD RGLGQG+ DN+     F  L+E   V S++    +HS+   SLLS
Sbjct: 976  QLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEVQ--DSHSTSYPSLLS 1035

Query: 1019 HCVGARLNYP-LHTFIAKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQS 1078
            H     LN P L   +A+   P    P  RSF PLA+ LPCD H+++    R L+  + +
Sbjct: 1036 HLTSMYLNAPALALPVARMQLPG---PGLRSFHPLASSLPCDFHLLNL---RTLQAEEDT 1095

Query: 1079 PEDPRFILIFHRRHWDSSY-CKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHE 1138
                   LI HR+ +D     K    NCT  +     + ++F    V   + TSL LL+ 
Sbjct: 1096 LPSAETALILHRKGFDCGLEAKNLGFNCTT-SQGKVALGSLFHGLDVVFLQPTSLTLLYP 1149

Query: 1139 DTEMLGYNEQSGDVAHEGQLHIPPMELRAYKLEV 1147
                L     S DV      ++ PME+  ++L +
Sbjct: 1156 ----LASPSNSTDV------YLEPMEIATFRLRL 1149

BLAST of MC08g1343 vs. ExPASy Swiss-Prot
Match: P27046 (Alpha-mannosidase 2 OS=Mus musculus OX=10090 GN=Man2a1 PE=1 SV=2)

HSP 1 Score: 719.9 bits (1857), Expect = 4.5e-206
Identity = 416/1058 (39.32%), Postives = 589/1058 (55.67%), Query Frame = 0

Query: 111  DITTKELYDKIEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEY 170
            D+   ++YD I F + DGG WKQG+ + Y  DEWD E L+VFVVPHSHNDPGW  T ++Y
Sbjct: 127  DVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEWDHEPLQVFVVPHSHNDPGWLKTFNDY 186

Query: 171  YERQSRRILDTIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVG 230
            +  +++ I + +V  L +DS RKF+W E+SYL KWW      K+EA  +L++NGQLEIV 
Sbjct: 187  FRDKTQYIFNNMVLKLKEDSSRKFMWSEISYLAKWWDIIDIPKKEAVKSLLQNGQLEIVT 246

Query: 231  GGWVMNDE-----------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFE 290
            GGWVM DE           + EG+ WL + +GV P++ WAIDPFG+SPTMAYLL+R GF 
Sbjct: 247  GGWVMPDEATPHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFS 306

Query: 291  NMLIQRTHYELKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAIC 350
            +MLIQR HY +KK  +LHK +EF WRQ+WD    TDI  HMMPFYSYDIPHTCGP+P IC
Sbjct: 307  HMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPFYSYDIPHTCGPDPKIC 366

Query: 351  CQFDFARSRGSIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDD 410
            CQFDF R  G  Y  CPW + P  I+  NVQ RA MLLDQYRKKS L+RT  LL PLGDD
Sbjct: 367  CQFDFKRLPGGRYG-CPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDD 426

Query: 411  FRYITIDEAEAQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEV 470
            FR+    E + Q +NY+ LF ++NS P L  + +FGTL DYF    D  EK   ++  E 
Sbjct: 427  FRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYF----DALEK---AVAAEK 486

Query: 471  GSSQISGFPSLSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGH 530
             SSQ S FP+LSGDFFTY+DR   YWSGY+ SRPF+K +DR++E  +RAAE++  L L  
Sbjct: 487  KSSQ-SVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQ 546

Query: 531  CQRSQCEKFPIGFSY-KLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLH-- 590
             Q+ +  KF     Y  LT ARRNL LFQHHD +TGTAKD VV DYG R+  SL  L   
Sbjct: 547  AQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKI 606

Query: 591  IFMSKGIEVLLGIRHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLE 650
            I  S  + +L   +  +SD +    E +  +S  D  P    I L  +  + +V +NP E
Sbjct: 607  IGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSSQDSLPQKIIIQLSAQEPRYLVVYNPFE 666

Query: 651  QTREEVVMIIVNMTDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQ 710
            Q R  VV I VN   V VL  +   +E QVS  + +D        + + +   +P LGL+
Sbjct: 667  QERHSVVSIRVNSATVKVLSDSGKPVEVQVSAVW-NDMRTISQAAYEVSFLAHIPPLGLK 726

Query: 711  TYYIANGLFGCEKPKPAKLNFFSKSTSLPCPTPYVCSKAD-GDVAEIQNQHQSLVFDVNH 770
             + I            A    ++          +V +  D GD   I+N   ++ FD   
Sbjct: 727  VFKILES--QSSSSHLADYVLYNNDGLAENGIFHVKNMVDAGDAITIENPFLAIWFD-RS 786

Query: 771  GLLQKVIKKSGS-QNVVNEEIAMYSSLG----SGAYLFNPIGDAKSIIEAGGLTV-IVEG 830
            GL++KV +K  S Q+ +  +   Y +      SGAYLF P G  +  +      V +  G
Sbjct: 787  GLMEKVRRKEDSRQHELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRG 846

Query: 831  PLMQEVYSYPKTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYK 890
             +  +V  +         ++H  RLY+    I+G  +E    V +  +   +RE+++R  
Sbjct: 847  RIYSDVTCF------LEHVTHKVRLYN-IQGIEGQSMEVSNIVNI--RNVHNREIVMRIS 906

Query: 891  TDVDNKRIYYSDLNGFQMSRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGV 950
            + ++N+  YY+DLNG+Q+  R +  K+PLQ N YPM ++A+++ +   R ++ S QSLG 
Sbjct: 907  SKINNQNRYYTDLNGYQIQPRRTMSKLPLQANVYPMCTMAYIQDAE-HRLTLLSAQSLGA 966

Query: 951  ASLKDGWLEIMLDRRLTRDDGRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLS 1010
            +S+  G +E+ +DRRL +DD RGLGQGV DN++   +F  L+E   +  +          
Sbjct: 967  SSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANLFRILLEKRSAVNMEEEKKSPVSY 1026

Query: 1011 ASLLSHCVGARLNYPLHTFIAKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKY 1070
             SLLSH   + LN+P    +     PS        F  L + LPCD+H+V+    R ++ 
Sbjct: 1027 PSLLSHMTSSFLNHPFLPMVLSGQLPSPAFELLSEFPLLQSSLPCDIHLVNL---RTIQS 1086

Query: 1071 SQQSPEDPRFILIFHRRHWDSSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNL 1130
                       LI HR+ +D  +                ++  +F  F V +   +SL+L
Sbjct: 1087 KMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKMSVLKLFNKFAVESLVPSSLSL 1146

Query: 1131 LHEDTEMLGYNEQSGDVAHEGQLHIPPMELRAYKLEVQ 1148
            +H             D  +  ++ + PME+  +++ ++
Sbjct: 1147 MHSPP----------DAQNMSEVSLSPMEISTFRIRLR 1148

BLAST of MC08g1343 vs. ExPASy Swiss-Prot
Match: P28494 (Alpha-mannosidase 2 OS=Rattus norvegicus OX=10116 GN=Man2a1 PE=1 SV=2)

HSP 1 Score: 716.8 bits (1849), Expect = 3.8e-205
Identity = 417/1058 (39.41%), Postives = 584/1058 (55.20%), Query Frame = 0

Query: 111  DITTKELYDKIEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEY 170
            D+   ++YD I F + DGG WKQG+ + Y  DEWD E L+VFVVPHSHNDPGW  T ++Y
Sbjct: 127  DVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEWDREPLQVFVVPHSHNDPGWLKTFNDY 186

Query: 171  YERQSRRILDTIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVG 230
            +  +++ I + +V  L +DS RKFIW E+SYL KWW    + K+EA  +L++NGQLEIV 
Sbjct: 187  FRDKTQYIFNNMVLKLKEDSSRKFIWSEISYLAKWWDIIDNPKKEAVKSLLQNGQLEIVT 246

Query: 231  GGWVMNDE-----------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFE 290
            GGWVM DE           + EG+ WL + +GV P++ WAIDPFG+SPTM YLL+R GF 
Sbjct: 247  GGWVMADEATTHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMTYLLKRAGFS 306

Query: 291  NMLIQRTHYELKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAIC 350
            +MLIQR HY +KK  +L K +EF WRQ+WD   TTDI  HMMPFYSYDIPHTCGP+P IC
Sbjct: 307  HMLIQRVHYSVKKHFSLQKTLEFFWRQNWDLGSTTDILCHMMPFYSYDIPHTCGPDPKIC 366

Query: 351  CQFDFARSRGSIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDD 410
            CQFDF R  G  Y  CPW + P  I+  NVQ RA MLLDQYRKKS L+RT  LL PLGDD
Sbjct: 367  CQFDFKRLPGGRYG-CPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDD 426

Query: 411  FRYITIDEAEAQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEV 470
            FR+    E + Q++NY+ LF ++NS P L  + +FGTL DYF    D  EK   S+  E 
Sbjct: 427  FRFSEYTEWDLQYRNYEQLFSYMNSQPHLKVKIQFGTLSDYF----DALEK---SVAAEK 486

Query: 471  GSSQISGFPSLSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGH 530
               Q S FP+LSGDFFTY+DR   YWSGY+ SRPF+K +DR++E  LR AE++  L L  
Sbjct: 487  KGGQ-SVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRLRTAEILYHLALKQ 546

Query: 531  CQRSQCEKFPIGFSY-KLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLH-- 590
             Q+ +  KF     Y  LT ARRNL LFQHHD +TGTAKD VV DYG R+  SL  L   
Sbjct: 547  AQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKI 606

Query: 591  IFMSKGIEVLLGIRHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLE 650
            I  S  + +L   +  +SD +    E +  +S  D  P    I L  +  + +V +NP E
Sbjct: 607  IGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSSQDSLPKKNIIQLSAQEPRYLVVYNPFE 666

Query: 651  QTREEVVMIIVNMTDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQ 710
            Q R  VV + VN   V VL      +E QVS  ++ D        + + +   +P LGL+
Sbjct: 667  QERHSVVSVRVNSATVKVLSDLGKAVEVQVSAVWK-DMRTTSQAAYEVAFLAHLPPLGLK 726

Query: 711  TYYIANGLFGCEKPKPAKLNFFSKSTSLPCPTPYVCSKAD-GDVAEIQNQHQSLVFDVNH 770
             Y I            A    ++          ++ +  D GD   I+N   +L FD   
Sbjct: 727  VYKILES--QSSSSHLADYFLYNNDGQAESGIFHMKNMVDSGDAITIENSFLTLGFD-RS 786

Query: 771  GLLQKVIKK-SGSQNVVNEEIAMYSSLG----SGAYLFNPIGDAKSIIEAGGLTV-IVEG 830
            GL++KV +K    Q  +  +   Y +      SGAYLF P G  +  +      V +  G
Sbjct: 787  GLMEKVRRKEDNKQQELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRTPFVRVTRG 846

Query: 831  PLMQEVYSYPKTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYK 890
             +  +V  +         ++H  RLY     I+G  +E    V++  +   +RE+++R  
Sbjct: 847  RIYSDVTCF------LEHVTHKVRLYH-IQGIEGQSMEVSNIVDI--RSVHNREIVMRIS 906

Query: 891  TDVDNKRIYYSDLNGFQMSRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGV 950
            + ++N+  YY+DLNG+Q+  R +  K+PLQ N YPM ++A+++ +   R ++ S QSLG 
Sbjct: 907  SKINNQNRYYTDLNGYQIQPRRTMAKLPLQANVYPMSTMAYIQDA-AHRLTLLSAQSLGA 966

Query: 951  ASLKDGWLEIMLDRRLTRDDGRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLS 1010
            +S+  G +E+ +DRRL +DD RGLGQGV DN++   +F  L+E      +          
Sbjct: 967  SSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANLFRILLEKRNGMNMEEDKKSPVSY 1026

Query: 1011 ASLLSHCVGARLNYPLHTFIAKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKY 1070
             SLLSH   A LN+P    +     PS        F  L + LPCD+H+V+ +  +    
Sbjct: 1027 PSLLSHMTSAFLNHPFLPMVLSGQLPSPAIELLSEFRLLQSSLPCDIHLVNLRTIQSKVG 1086

Query: 1071 SQQSPEDPRFILIFHRRHWDSSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNL 1130
               S E     LI HR+ +D      A            ++  +F +F V +   +SL+L
Sbjct: 1087 KGYSDE---AALILHRKVFDCQLSSRAMGLPCSTTQGKMSIPKLFNNFAVESFIPSSLSL 1146

Query: 1131 LHEDTEMLGYNEQSGDVAHEGQLHIPPMELRAYKLEVQ 1148
            +H             D  +  ++ + PME+   ++ ++
Sbjct: 1147 MHSPP----------DAQNTSEVSLSPMEISTSRIRLR 1148

BLAST of MC08g1343 vs. ExPASy Swiss-Prot
Match: Q8BRK9 (Alpha-mannosidase 2x OS=Mus musculus OX=10090 GN=Man2a2 PE=1 SV=2)

HSP 1 Score: 716.1 bits (1847), Expect = 6.5e-205
Identity = 419/1054 (39.75%), Postives = 606/1054 (57.50%), Query Frame = 0

Query: 119  DKIEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRI 178
            + + F +V+GG W+QG+ ++Y+ ++WD+E L+VFVVPHSHNDPGW  T D+YY  Q++ I
Sbjct: 136  EDLPFDNVEGGVWRQGFDISYSPNDWDTEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHI 195

Query: 179  LDTIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE 238
            L+++V  L +D RR+F+W E+S+  KWW + S +KR A   LV NGQLEI  GGWVM DE
Sbjct: 196  LNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDE 255

Query: 239  -----------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTH 298
                       + EG+ WL   +G  P++ WA+DPFG+S TM YLLRR    +MLIQR H
Sbjct: 256  ANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVH 315

Query: 299  YELKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARS 358
            Y +KK  A   ++EF WRQ WD++ +TDIF HMMPFYSYD+PHTCGP+P ICCQFDF R 
Sbjct: 316  YAIKKHFAATHSLEFMWRQMWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRL 375

Query: 359  RGSIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDE 418
             G     CPW++ P  I   NV +RA +LLDQYRKKS L+R+N LL+PLGDDFRY    E
Sbjct: 376  PGGRIN-CPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQE 435

Query: 419  AEAQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGF 478
             +AQF NYQ LFD +NS P  + +A+FGTL +YF  L        Y   G    ++  GF
Sbjct: 436  WDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDAL--------YKRTGVEPGARPPGF 495

Query: 479  PSLSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRS-QCE 538
            P LSGDFF+Y+DR+  YW+GYY SRPF+K++DRVLE  LR AE++ +L L H +RS    
Sbjct: 496  PVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLALAHARRSGLAG 555

Query: 539  KFPIGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDL-HIFMSKGIEV 598
            ++P+     LT ARR L LFQHHD +TGTAK+ VV DYG+R+  SL  L  + ++    +
Sbjct: 556  QYPLSDFALLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYL 615

Query: 599  LLGIRHEKS-DHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVM 658
            +LG +   S D  +   + +  R  +D  P    I L   S + VV FNPLEQ R  VV 
Sbjct: 616  VLGDQETYSFDPGTPFLQMDDSRVSHDALPERTVIRLD-SSPRFVVVFNPLEQERLSVVS 675

Query: 659  IIVNMTDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYI---A 718
            ++VN   V VL      L  Q+S  +           +++   + +P LGL    +    
Sbjct: 676  LLVNSPRVRVLSEEGQPLSVQISVHWS-SATDMVPDVYQVSVPVRLPGLGLGVLQLQPDL 735

Query: 719  NGLFGCEKPKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKV 778
            +G +  +      LN    S S     P     +      I N++  + F    GLL+ +
Sbjct: 736  DGPYTLQSSVRVYLNGVKLSVSRQSAFPVRVVDSGASDFAISNRYMQVWFSGLTGLLKSI 795

Query: 779  IKKSGSQNVVNE-EIAMY----SSLGSGAYLFNPIGDAKSII-EAGGLTVIVEGPLMQEV 838
             +    Q    E E  +Y    S   SGAYLF P  +AK  + +   +  + EGP   EV
Sbjct: 796  RRVDEEQEQQMELEFLVYGTRTSKDKSGAYLFLPDSEAKPYVPKKPPVLRVTEGPFFSEV 855

Query: 839  YSYPKTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNK 898
              Y              RLY+    ++G  ++  + V++  +++ ++EL +R  TD+D++
Sbjct: 856  AVY------YEHFHQVIRLYNLPG-VEGLSLDMSFQVDI--RDYVNKELALRIHTDIDSQ 915

Query: 899  RIYYSDLNGFQMSRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDG 958
              +++DLNGFQ+  R+   K+PLQ N+YPMP +A+++ S  +R ++H+ Q+LGV+SL +G
Sbjct: 916  GTFFTDLNGFQIQPRQYLKKLPLQANFYPMPVMAYIQDSQ-RRLTLHTAQALGVSSLGNG 975

Query: 959  WLEIMLDRRLTRDDGRGLGQGVTDNRVMNVVFHTLIE--SNVSSKLNPVPNHSSLSASLL 1018
             LE++LDRRL +DD RGLGQG+ DN++    F  L+E  + +S +++     S+   SLL
Sbjct: 976  QLEVILDRRLMQDDNRGLGQGLKDNKITCNRFRLLLERRTTMSPEVHQEQERSTSYPSLL 1035

Query: 1019 SHCVGARLNYPLHTFIAKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQS 1078
            SH     L+ P       K Q +S  P  RSF PLA+PLPCD H+++ ++  P + +  +
Sbjct: 1036 SHLTSMYLSTPPLVLPVAKRQGTS--PALRSFHPLASPLPCDFHLLNLRM-LPAEDTLPA 1095

Query: 1079 PEDPRFILIFHRRHWDSSY-CKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHE 1138
             +     LI HR+ +D     K    NCT  +     + ++F    V+  + TSL LL+ 
Sbjct: 1096 TDS---ALILHRKGFDCGLEAKNLGFNCTT-SQGKLALGSLFHGLDVTFLQPTSLTLLYP 1151

Query: 1139 DTEMLGYNEQSGDVAHEGQLHIPPMELRAYKLEV 1147
                L     S D++ E      PME+  ++L +
Sbjct: 1156 ----LASPSNSTDISLE------PMEISTFRLRL 1151

BLAST of MC08g1343 vs. NCBI nr
Match: XP_022150082.1 (alpha-mannosidase 2 [Momordica charantia])

HSP 1 Score: 2333 bits (6045), Expect = 0.0
Identity = 1148/1160 (98.97%), Postives = 1149/1160 (99.05%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI
Sbjct: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK
Sbjct: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD
Sbjct: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELALHKN+EFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELALHKNLEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE
Sbjct: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS
Sbjct: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP
Sbjct: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM
Sbjct: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK
Sbjct: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN
Sbjct: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS
Sbjct: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR
Sbjct: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI
Sbjct: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD
Sbjct: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPMELRAYKLEVQPR
Sbjct: 1141 GQLHIPPMELRAYKLEVQPR 1160

BLAST of MC08g1343 vs. NCBI nr
Match: KAG6580996.1 (Alpha-mannosidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2105 bits (5453), Expect = 0.0
Identity = 1016/1160 (87.59%), Postives = 1082/1160 (93.28%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFT GG  RRGGWASSILP S+ SP+AK KH RK+RRRLA+RDFIFAN FTIG L+
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            S IFFFIVL+RYGVPKPISSH +SHATRSHRPR+PIVSENWN EVLS+ VDITTK+LYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSENWNSEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLDVDGGPWKQGW+VTY GDEWDSEKLK+FVVPHSHNDPGWKLTVDEYYE+QSR IL+
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIV+ALS+DSRRKFIWEEMSYLEKWWRDASDEK+E+FVTLVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGNMWL ET+G+VPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELA HKN+EF WRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            S YELCPWR DPVEIN+ENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRY++IDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFD+INSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTY+DRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEM+LALLLG CQRSQCEK P
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RHEKSD N  QFE EQ+RSKYD Q VH+AIDLQ  +YQSVVFFNPLEQTREEV MIIVN 
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            TDV+VLDSNWTCL+SQ+SPEFQHDKA  FTGRHRLHWKISVPALGLQTYYIANGLF CEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRLHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKL FFS STSLPC TPY+CSKAD DVA I+NQHQSLVFD  HGLL K+I K+GSQN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VVNEEIA+Y+S GSGAYLF P GDA+SII+AGGLTVI EGPLMQEVYSYPKT WEKSPIS
Sbjct: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSG + IQ H+IE EYHVELLGKEFDD+ELIVRYKTD+DNKRI+YSDLNGFQ+SR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFS+HS+QSLGVASLKDGWLEIMLDRRL+ DD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGV DNR MNV+FH  +ES+VS+KLNPV N+S LS SLLSHC+GARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHCIGARLNYPLHTFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQ SSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRF+L+FHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLVFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRF+DEP N+FNMFK   VSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPME++AYKLE++PR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of MC08g1343 vs. NCBI nr
Match: KAG7017738.1 (Alpha-mannosidase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2102 bits (5447), Expect = 0.0
Identity = 1015/1160 (87.50%), Postives = 1081/1160 (93.19%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFT GG  RRGGWASSILP S+ SP+AK KH RK+RRRLA+RDFIFAN FTIG L+
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            S IFFFIVL+RYGVPKPISSH +SHATRSHRPR+PIVSENWN EVLS+ VDITTK+LYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSENWNSEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLDVDGGPWKQGW+VTY GDEWDSEKLK+FVVPHSHNDPGWKLTVDEYYE+QSR IL+
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIV+ALS+DSRRKFIWEEMSYLEKWWRDASDEK+E+FVTLVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGNMWL ET+G+VPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELA HKN+EF WRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            S YELCPWR DPVEIN+ENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRY++IDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFD+INSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTY+DRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEM+LALLLG CQRSQCEK P
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RHEKSD N  QFE EQ+RSKYD Q VH+AIDLQ  +YQSVVFFNPLEQTREEV MIIVN 
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            TDV+VLDSNWTCL+SQ+SPEFQHDKA  FTGRHRLHWKISVPALGLQTYYIANGLF CEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRLHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKL FFS STSLPC TPY+CSKAD DVA I+NQHQSLVFD  HGLL K+I K+GSQN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VVNEEIA+Y+S GSGAYLF P GDA+SII+AGGLTVI EGPLMQEVYSYPKT WEKSPIS
Sbjct: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSG + IQ H+IE EYHVELLGKEFDD+ELIVRYKTD+DNKRI+YSDLNGFQ+SR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFS+HS+QSLGVASLKDGWLEIMLDRRL+ DD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGV DNR MNV+FH  +ES+VS+KLNPV N+S LS SLLSHC+ ARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHCISARLNYPLHTFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQ SSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRF+L+FHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLVFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRF+DEP N+FNMFK   VSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPME++AYKLE++PR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of MC08g1343 vs. NCBI nr
Match: XP_023526938.1 (alpha-mannosidase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2101 bits (5444), Expect = 0.0
Identity = 1015/1160 (87.50%), Postives = 1081/1160 (93.19%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFT GG  RRGGWASSILP S+ SP+AK KH RK+RRRLA+RDFIFAN FTIG L+
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            S IFFFIVL+RYGVPKPISSH +SHATRSHRPR+PIVSENWN EVLS+ VDITTK+LYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSENWNNEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLDVDGGPWKQGW+VTY GDEWDSEKLK+FVVPHSHNDPGWKLTVDEYYE+QSR IL+
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIV+ALS+DSRRKFIWEEMSYLEKWWRDASDEK+E+FVTLVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGNMWL ET+G+VPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELA HKN+EF WRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            S YELCPWR DPVEIN+ENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRY++IDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFD+INSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTY+DRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEM+LALLLG CQRSQCEK P
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RHEKSD N  QFE EQ+RSKYD Q VH+AIDL   +YQSVVFFNPLEQTREEV MIIVN 
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLIEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            TDV+VLDSNWTCL+SQ+SPEFQHDKA  FTGRHR+HWKISVPALGLQTYYIANGLF CEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKL FFS STSLPC TPY+CSKAD DVA I+NQHQSLVFD  HGLL K+I K+GSQN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VVNEEIA+Y+S GSGAYLF P GDA+SII+AGGLTVI EGPLMQEVYSYPKT WEKSPIS
Sbjct: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSG + IQ H+IE EYHVELLGKEFDD+ELIVRYKTD+DNKRI+YSDLNGFQ+SR
Sbjct: 841  HSTRLYSGGNPIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFS+HS+QSLGVASLKDGWLEIMLDRRL+ DD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGV DNR MNV+FH  +ES+VS+KLNPV N+S LS SLLSHC+GARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHCIGARLNYPLHTFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQ SSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRF+LIFHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRF+DEP N+FNMFK   VSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPME++AYKLE++PR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of MC08g1343 vs. NCBI nr
Match: XP_038903640.1 (alpha-mannosidase 2 [Benincasa hispida])

HSP 1 Score: 2101 bits (5443), Expect = 0.0
Identity = 1013/1160 (87.33%), Postives = 1080/1160 (93.10%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFT G  GRRGGWASSILP S+ SP+AK KH RK+RRRLA+RDFIF+NFFTIG LI
Sbjct: 1    MAFSSFTGGSGGRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFSNFFTIGLLI 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            S  FFFIVLLRYGVPKPISS F+SHA RSHRPR+PIVSENWN EVLS+NVDITTKELYDK
Sbjct: 61   SFFFFFIVLLRYGVPKPISSPFKSHAIRSHRPRKPIVSENWNSEVLSSNVDITTKELYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLD+DGGPWKQGW+VTY GDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSR ILD
Sbjct: 121  IEFLDIDGGPWKQGWRVTYKGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRHILD 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIVEALS+DSRRKFIWEEMSYLEKWWRDAS+EK+E+F TLVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVEALSQDSRRKFIWEEMSYLEKWWRDASEEKKESFATLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGNMWLNETIG+VPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLNETIGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELA HKN+EF WRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            S+YELCPWR DPVEIN+ENVQERAM LLDQYRKKSVLYRTNTLLIPLGDDFRYI+IDEAE
Sbjct: 361  SLYELCPWRQDPVEINKENVQERAMTLLDQYRKKSVLYRTNTLLIPLGDDFRYISIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFD+INSNPSLNAE KFGTLEDYF+TLRDEAEKINYSLPGEVGSSQ+SGFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAETKFGTLEDYFQTLRDEAEKINYSLPGEVGSSQVSGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTY+DRQ+DYWSGYYVSRPFFKAVDRVLE+TLRAAEM+LALLLG CQR QCEK P
Sbjct: 481  LSGDFFTYADRQEDYWSGYYVSRPFFKAVDRVLERTLRAAEMILALLLGPCQRPQCEKLP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  LGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RH+KSD N  QFE EQ RSKYD QPVH+AIDLQ  +YQSVVFFNP+EQTREEV MIIVN 
Sbjct: 601  RHDKSDQNPSQFEPEQTRSKYDAQPVHKAIDLQEGTYQSVVFFNPIEQTREEVAMIIVNW 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            TDV+VLDSNW C++SQ+SPEFQHDKAK FTGRHR+HWKISVPALGLQTYYIANGLF CEK
Sbjct: 661  TDVTVLDSNWMCVQSQISPEFQHDKAKVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKL  F+ STS+PCPTPYVCSKADGD+AEI+N+HQSLVFDV HGLLQK+I K+GS+N
Sbjct: 721  PKPAKLKLFTTSTSIPCPTPYVCSKADGDMAEIENEHQSLVFDVKHGLLQKIINKNGSEN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VVNEEI++YSS GSGAYLF P GDAKSIIEAGGLTVI EGPLMQEV+SYPKTEW  SPIS
Sbjct: 781  VVNEEISLYSSWGSGAYLFKPTGDAKSIIEAGGLTVITEGPLMQEVFSYPKTEWVNSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSG + +Q H+IE EYHVELLG+EFDDRELIVRYKTD+DNKRI+YSDLNG QMSR
Sbjct: 841  HSTRLYSGANTMQEHLIEMEYHVELLGREFDDRELIVRYKTDIDNKRIFYSDLNGLQMSR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFM+GSNGQRFS+HSKQSLGVASLKDGWLEIMLDRRL RDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMQGSNGQRFSVHSKQSLGVASLKDGWLEIMLDRRLVRDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGVTDNR MNV FH L+ESNVS+KLNPV N+  LS SLLSHC+GARLNYPLHTFI
Sbjct: 961  GRGLGQGVTDNRAMNVAFHILLESNVSTKLNPVSNYFPLSPSLLSHCIGARLNYPLHTFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQ SSVQP  RSFSPLAAPLPCDLH+VSFKVPRPLKYSQQSPEDPRF+LIFHRRHWD
Sbjct: 1021 AKKPQASSVQPTLRSFSPLAAPLPCDLHVVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTR ADEPFN+FNMFK   VS A+ATSLNLLHED+EMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRVADEPFNIFNMFKGLAVSKAQATSLNLLHEDSEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPME+RAYKLE++PR
Sbjct: 1141 GQLHIPPMEVRAYKLELKPR 1160

BLAST of MC08g1343 vs. ExPASy TrEMBL
Match: A0A6J1D9R2 (Alpha-mannosidase OS=Momordica charantia OX=3673 GN=LOC111018346 PE=3 SV=1)

HSP 1 Score: 2333 bits (6045), Expect = 0.0
Identity = 1148/1160 (98.97%), Postives = 1149/1160 (99.05%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI
Sbjct: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK
Sbjct: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD
Sbjct: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELALHKN+EFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELALHKNLEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE
Sbjct: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS
Sbjct: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP
Sbjct: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM
Sbjct: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK
Sbjct: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN
Sbjct: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS
Sbjct: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR
Sbjct: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI
Sbjct: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD
Sbjct: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPMELRAYKLEVQPR
Sbjct: 1141 GQLHIPPMELRAYKLEVQPR 1160

BLAST of MC08g1343 vs. ExPASy TrEMBL
Match: A0A6J1F199 (Alpha-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111441463 PE=3 SV=1)

HSP 1 Score: 2095 bits (5429), Expect = 0.0
Identity = 1013/1160 (87.33%), Postives = 1080/1160 (93.10%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFT GG  RRGGWASSILP S+ SP+AK KH RK+RRRLA+RDFIFAN FTIG L+
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            S IFFFIVL+RYGVPKPISSH +SHATRSHRPR+PIVS+NWN EVLS+ VDITTK+LYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSKNWNSEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLDVDGGPWKQGW+VTY GDEWDSEKLK+FVVPHSHNDPGWKLTVDEYYE+QSR IL+
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIV+ALS+DSRRKFIWEEMSYLEKWWRDASDEK+E+FVTLVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGNMWL ET+G+VPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELA HKN+EF WRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            S YELCPWR DPVEIN+ENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRY++IDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFD+INSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTY+DRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEM+LALLLG CQRSQCEK P
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RHEKSD N  QFE EQ+RSKYD Q VH+AIDLQ  +YQSVVFFNPLEQTREEV MIIVN 
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            TDV+VLDSNWTCL+SQ+SPEFQHDKA  FTGRHRLHWKISVPALGLQTYYIANGLF CEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRLHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKL FFS STSLPC TPY+CSKAD DVA I+NQHQSLVFD  HGLL K+I K+GSQN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VV+EEIA+Y+S GSGAYLF P GDA+SII+AGGLTVI EGPLMQEVYSYPKT WEKSPIS
Sbjct: 781  VVSEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSG + IQ H+IE EYHVELLGKEFDD+ELIVRYKTD+DNKRI+YSDLNGFQ+SR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFS+HS+QSLGVASLKDGWLEIMLDRRL+ DD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGV DNR MNV+FH  +ES+VS+KLNPV N+S LS SLLSH +GARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHGIGARLNYPLHTFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQ SSVQPPPRSFSPLA PLPCDLHIVSFKVPRPLKYSQQSPEDPRF+LIFHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLATPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRF+DEP N+FNMFK   VSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPME++AYKLE++PR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of MC08g1343 vs. ExPASy TrEMBL
Match: A0A5A7UER4 (Alpha-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00900 PE=3 SV=1)

HSP 1 Score: 2095 bits (5427), Expect = 0.0
Identity = 1010/1157 (87.29%), Postives = 1073/1157 (92.74%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFT G   RRGGWA SILP S+ SP+AK KH RK+RRRLA+RDFIF+NFFTIG LI
Sbjct: 1    MAFSSFTGGSGARRGGWAGSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFSNFFTIGLLI 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            S  FFFIVLLRYGVPKPISS F+SHA RSHRPR+PIVSENWN EVLS+NVDITTKELYDK
Sbjct: 61   SFFFFFIVLLRYGVPKPISSPFKSHAVRSHRPRKPIVSENWNSEVLSSNVDITTKELYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLD+DGGPWKQGW+VTY GDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSR ILD
Sbjct: 121  IEFLDIDGGPWKQGWRVTYKGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRHILD 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIVEALS+DSRRKFIWEEMSYLEKWWRDASDEK+E+F  LVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVEALSRDSRRKFIWEEMSYLEKWWRDASDEKKESFAALVKNGQLEIVGGGWVMNDEVI 240

Query: 241  ------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYELKK 300
                  MAEGN+WLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYELKK
Sbjct: 241  YSLACEMAEGNIWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYELKK 300

Query: 301  ELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSIY 360
            ELALHKN+EF WRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGS+Y
Sbjct: 301  ELALHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSLY 360

Query: 361  ELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAEAQF 420
            ELCPWR DPVEIN+ENVQERA  LLDQYRKKSVLYRTNTLLIPLGDDFRYI IDEAEAQF
Sbjct: 361  ELCPWRQDPVEINKENVQERATTLLDQYRKKSVLYRTNTLLIPLGDDFRYINIDEAEAQF 420

Query: 421  KNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPSLSG 480
            KNYQLLFD+INS PSLNAEA FGTLEDYFRTLRDEAEKINYSLPGEVGSS + GFPSLSG
Sbjct: 421  KNYQLLFDYINSTPSLNAEANFGTLEDYFRTLRDEAEKINYSLPGEVGSSLVGGFPSLSG 480

Query: 481  DFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFPIGF 540
            DFFTY+DRQ+DYWSGYYVSRPFFKAVDRVLE+TLRAAEMMLALLL  CQRSQCEK P+GF
Sbjct: 481  DFFTYADRQEDYWSGYYVSRPFFKAVDRVLERTLRAAEMMLALLLAPCQRSQCEKLPLGF 540

Query: 541  SYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGIRHE 600
            SYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSK IEVLLGIRH+
Sbjct: 541  SYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGIRMHTSLQDLHIFMSKAIEVLLGIRHD 600

Query: 601  KSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNMTDV 660
            KSD N  QFE EQMRSKYD QPVH++IDLQ  +YQSV+FFNPLEQTREEV M+IVN T+V
Sbjct: 601  KSDQNPSQFEPEQMRSKYDAQPVHKSIDLQEGTYQSVIFFNPLEQTREEVAMVIVNRTEV 660

Query: 661  SVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEKPKP 720
            +VLDSNWTC++SQ+SPEFQHDKAK FTGRHR+HWKISVPALGLQTYYIANGLF CEKPKP
Sbjct: 661  TVLDSNWTCVQSQISPEFQHDKAKVFTGRHRIHWKISVPALGLQTYYIANGLFDCEKPKP 720

Query: 721  AKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQNVVN 780
            AKL FFSKSTSLPCPTPYVCSKAD DVAEI+NQHQSLVFDV HGLLQKVI K+GS+NVVN
Sbjct: 721  AKLKFFSKSTSLPCPTPYVCSKADSDVAEIENQHQSLVFDVKHGLLQKVINKNGSENVVN 780

Query: 781  EEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPISHST 840
            EEIAMYSS GSGAYLF P G+AKSI E GGLTVI EGPLMQEV+SYPKT WE SPISHST
Sbjct: 781  EEIAMYSSWGSGAYLFKPTGEAKSITEEGGLTVITEGPLMQEVFSYPKTGWEPSPISHST 840

Query: 841  RLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSRRES 900
            RLYSG + IQ H+IE EYHVELLG+E+DDRELIVRYKTD+DNKRI+YSDLNG QMSRRES
Sbjct: 841  RLYSGGNSIQEHLIEMEYHVELLGREYDDRELIVRYKTDIDNKRIFYSDLNGLQMSRRES 900

Query: 901  YDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDDGRG 960
            YDKIPLQGNYYPMPSLAFMEGSNGQRFS+HS+QSLGVASLKDGWLEIMLDRRL RDDGRG
Sbjct: 901  YDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLYRDDGRG 960

Query: 961  LGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFIAKK 1020
            LGQGVTDNR MNVVFH L+ESNVS+KLNPV ++S LS SLLSHC+G  LNYPLH FIAKK
Sbjct: 961  LGQGVTDNRAMNVVFHILLESNVSTKLNPVSSYSPLSPSLLSHCIGGHLNYPLHAFIAKK 1020

Query: 1021 PQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWDSSY 1080
            PQPSS+QPP RSFSPLAAPLPCDLHIVSFKVPRPLKY+QQSPEDPRF+LIFHRRHWDSSY
Sbjct: 1021 PQPSSLQPPSRSFSPLAAPLPCDLHIVSFKVPRPLKYTQQSPEDPRFLLIFHRRHWDSSY 1080

Query: 1081 CKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHEGQL 1140
            CKTARSNCT+ ADEPFN+FNMFK   VS ARA+SLNLLHEDTEMLGYNEQSGDV HEGQL
Sbjct: 1081 CKTARSNCTKAADEPFNIFNMFKGLAVSKARASSLNLLHEDTEMLGYNEQSGDVGHEGQL 1140

Query: 1141 HIPPMELRAYKLEVQPR 1149
            HIPPME+RAYKLE++PR
Sbjct: 1141 HIPPMEVRAYKLELKPR 1157

BLAST of MC08g1343 vs. ExPASy TrEMBL
Match: A0A1C9J7V4 (Alpha-mannosidase OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 2094 bits (5425), Expect = 0.0
Identity = 1010/1160 (87.07%), Postives = 1073/1160 (92.50%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFT G   RRGGWA SILP S+ SP+AK KH RK+RRRLA+RDFIF+NFFTIG LI
Sbjct: 1    MAFSSFTGGSGARRGGWAGSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFSNFFTIGLLI 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            S  FFFIVLLRYGVPKPISS F+SHA RSHRPR+PIVSENWN EVLS+NVDITTKELYDK
Sbjct: 61   SFFFFFIVLLRYGVPKPISSPFKSHAVRSHRPRKPIVSENWNSEVLSSNVDITTKELYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLD+DGGPWKQGW+VTY GDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSR ILD
Sbjct: 121  IEFLDIDGGPWKQGWRVTYKGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRHILD 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIVEALS+DSRRKFIWEEMSYLEKWWRDASDEK+E+F  LVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVEALSRDSRRKFIWEEMSYLEKWWRDASDEKKESFAALVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGN+WLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNIWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELALHKN+EF WRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELALHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            S+YELCPWR DPVEIN+ENVQERA  LLDQYRKKSVLYRTNTLLIPLGDDFRYI IDEAE
Sbjct: 361  SLYELCPWRQDPVEINKENVQERATTLLDQYRKKSVLYRTNTLLIPLGDDFRYINIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFD+INS PSLNAEA FGTLEDYFRTLRDEAEKINYSLPGEVGSS + GFPS
Sbjct: 421  AQFKNYQLLFDYINSTPSLNAEANFGTLEDYFRTLRDEAEKINYSLPGEVGSSLVGGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTY+DRQ+DYWSGYYVSRPFFKAVDRVLE+TLRAAEMMLALLL  CQRSQCEK P
Sbjct: 481  LSGDFFTYADRQEDYWSGYYVSRPFFKAVDRVLERTLRAAEMMLALLLAPCQRSQCEKLP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSK IEVLLGI
Sbjct: 541  LGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGIRMHTSLQDLHIFMSKAIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RH+KSD N  QFE EQMRSKYD QPVH++IDLQ  +YQSV+FFNPLEQTREEV M+IVN 
Sbjct: 601  RHDKSDQNPSQFEPEQMRSKYDAQPVHKSIDLQEGTYQSVIFFNPLEQTREEVAMVIVNR 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            T+V+VLDSNWTC++SQ+SPEFQHDKAK FTGRHR+HWKISVPALGLQTYYIANGLF CEK
Sbjct: 661  TEVTVLDSNWTCVQSQISPEFQHDKAKVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKL FFSKSTSLPCPTPYVCSKAD DVAEI+NQHQSLVFDV HGLLQKVI K+GS+N
Sbjct: 721  PKPAKLKFFSKSTSLPCPTPYVCSKADSDVAEIENQHQSLVFDVKHGLLQKVINKNGSEN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VVNEEIAMYSS GSGAYLF P G+AKSI E GGLTVI EGPLMQEV+SYPKT WE SPIS
Sbjct: 781  VVNEEIAMYSSWGSGAYLFKPTGEAKSITEEGGLTVITEGPLMQEVFSYPKTGWEPSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSG + IQ H+IE EYHVELLG+E+DDRELIVRYKTD+DNKRI+YSDLNG QMSR
Sbjct: 841  HSTRLYSGGNSIQEHLIEMEYHVELLGREYDDRELIVRYKTDIDNKRIFYSDLNGLQMSR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFS+HS+QSLGVASLKDGWLEIMLDRRL RDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLYRDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGVTDNR MNVVFH L+ESNVS+KLNPV ++S LS SLLSHC+G  LNYPLH FI
Sbjct: 961  GRGLGQGVTDNRAMNVVFHILLESNVSTKLNPVSSYSPLSPSLLSHCIGGHLNYPLHAFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQPSS+QPP RSFSPLAAPLPCDLHIVSFKVPRPLKY+QQSPEDPRF+LIFHRRHWD
Sbjct: 1021 AKKPQPSSLQPPSRSFSPLAAPLPCDLHIVSFKVPRPLKYTQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCT+ ADEPFN+FNMFK   VS ARA+SLNLLHEDTEMLGYNEQSGDV HE
Sbjct: 1081 SSYCKTARSNCTKVADEPFNIFNMFKGLAVSKARASSLNLLHEDTEMLGYNEQSGDVGHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPME+RAYKLE++PR
Sbjct: 1141 GQLHIPPMEVRAYKLELKPR 1160

BLAST of MC08g1343 vs. ExPASy TrEMBL
Match: A0A1S3B8V9 (Alpha-mannosidase OS=Cucumis melo OX=3656 GN=LOC103487047 PE=3 SV=1)

HSP 1 Score: 2093 bits (5424), Expect = 0.0
Identity = 1010/1160 (87.07%), Postives = 1073/1160 (92.50%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRRGGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANFFTIGFLI 60
            MAFSSFT G   RRGGWA SILP S+ SP+AK KH RK+RRRLA+RDFIF+NFFTIG LI
Sbjct: 1    MAFSSFTGGSGARRGGWAGSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFSNFFTIGLLI 60

Query: 61   SLIFFFIVLLRYGVPKPISSHFRSHATRSHRPRRPIVSENWNREVLSANVDITTKELYDK 120
            S  FFFIVLLRYGVPKPISS F+SHA RSHRPR+PIVSENWN EVLS+NVDITTKELYDK
Sbjct: 61   SFFFFFIVLLRYGVPKPISSPFKSHAVRSHRPRKPIVSENWNSEVLSSNVDITTKELYDK 120

Query: 121  IEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRRILD 180
            IEFLD+DGGPWKQGW+VTY GDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSR ILD
Sbjct: 121  IEFLDIDGGPWKQGWRVTYKGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRHILD 180

Query: 181  TIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-- 240
            TIVEALS+DSRRKFIWEEMSYLEKWWRDASDEK+E+F  LVKNGQLEIVGGGWVMNDE  
Sbjct: 181  TIVEALSRDSRRKFIWEEMSYLEKWWRDASDEKKESFAALVKNGQLEIVGGGWVMNDEAN 240

Query: 241  ---------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
                     MAEGN+WLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNIWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELALHKN+EF WRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELALHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAE 420
            S+YELCPWR DPVEIN+ENVQERA  LLDQYRKKSVLYRTNTLLIPLGDDFRYI IDEAE
Sbjct: 361  SLYELCPWRQDPVEINKENVQERATTLLDQYRKKSVLYRTNTLLIPLGDDFRYINIDEAE 420

Query: 421  AQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFD+INS PSLNAEA FGTLEDYFRTLRDEAEKINYSLPGEVGSS + GFPS
Sbjct: 421  AQFKNYQLLFDYINSTPSLNAEANFGTLEDYFRTLRDEAEKINYSLPGEVGSSLVGGFPS 480

Query: 481  LSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFP 540
            LSGDFFTY+DRQ+DYWSGYYVSRPFFKAVDRVLE+TLRAAEMMLALLL  CQRSQCEK P
Sbjct: 481  LSGDFFTYADRQEDYWSGYYVSRPFFKAVDRVLERTLRAAEMMLALLLAPCQRSQCEKLP 540

Query: 541  IGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSK IEVLLGI
Sbjct: 541  LGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGIRMHTSLQDLHIFMSKAIEVLLGI 600

Query: 601  RHEKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNM 660
            RH+KSD N  QFE EQMRSKYD QPVH++IDLQ  +YQSV+FFNPLEQTREEV M+IVN 
Sbjct: 601  RHDKSDQNPSQFEPEQMRSKYDAQPVHKSIDLQEGTYQSVIFFNPLEQTREEVAMVIVNR 660

Query: 661  TDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYYIANGLFGCEK 720
            T+V+VLDSNWTC++SQ+SPEFQHDKAK FTGRHR+HWKISVPALGLQTYYIANGLF CEK
Sbjct: 661  TEVTVLDSNWTCVQSQISPEFQHDKAKVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVIKKSGSQN 780
            PKPAKL FFSKSTSLPCPTPYVCSKAD DVAEI+NQHQSLVFDV HGLLQKVI K+GS+N
Sbjct: 721  PKPAKLKFFSKSTSLPCPTPYVCSKADSDVAEIENQHQSLVFDVKHGLLQKVINKNGSEN 780

Query: 781  VVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYPKTEWEKSPIS 840
            VVNEEIAMYSS GSGAYLF P G+AKSI E GGLTVI EGPLMQEV+SYPKT WE SPIS
Sbjct: 781  VVNEEIAMYSSWGSGAYLFKPTGEAKSITEEGGLTVITEGPLMQEVFSYPKTGWEPSPIS 840

Query: 841  HSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYYSDLNGFQMSR 900
            HSTRLYSG + IQ H+IE EYHVELLG+E+DDRELIVRYKTD+DNKRI+YSDLNG QMSR
Sbjct: 841  HSTRLYSGGNSIQEHLIEMEYHVELLGREYDDRELIVRYKTDIDNKRIFYSDLNGLQMSR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEIMLDRRLTRDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFS+HS+QSLGVASLKDGWLEIMLDRRL RDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLYRDD 960

Query: 961  GRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGARLNYPLHTFI 1020
            GRGLGQGVTDNR MNVVFH L+ESNVS+KLNPV ++S LS SLLSHC+G  LNYPLH FI
Sbjct: 961  GRGLGQGVTDNRAMNVVFHILLESNVSTKLNPVSSYSPLSPSLLSHCIGGHLNYPLHAFI 1020

Query: 1021 AKKPQPSSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFILIFHRRHWD 1080
            AKKPQPSS+QPP RSFSPLAAPLPCDLHIVSFKVPRPLKY+QQSPEDPRF+LIFHRRHWD
Sbjct: 1021 AKKPQPSSLQPPSRSFSPLAAPLPCDLHIVSFKVPRPLKYTQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCT+ ADEPFN+FNMFK   VS ARA+SLNLLHEDTEMLGYNEQSGDV HE
Sbjct: 1081 SSYCKTARSNCTKAADEPFNIFNMFKGLAVSKARASSLNLLHEDTEMLGYNEQSGDVGHE 1140

Query: 1141 GQLHIPPMELRAYKLEVQPR 1149
            GQLHIPPME+RAYKLE++PR
Sbjct: 1141 GQLHIPPMEVRAYKLELKPR 1160

BLAST of MC08g1343 vs. TAIR 10
Match: AT5G14950.1 (golgi alpha-mannosidase II )

HSP 1 Score: 1654.0 bits (4282), Expect = 0.0e+00
Identity = 817/1176 (69.47%), Postives = 941/1176 (80.02%), Query Frame = 0

Query: 1    MAFSSFTRGGSGRR-------GGWASSILPSSTTSPNAKQKHVRKFRRRLAVRDFIFANF 60
            M FSS+   G+ RR       GGW  S+LP  T    +K    RK R+R  V +FIFANF
Sbjct: 1    MPFSSYI--GNSRRSSTGGGTGGWGQSLLP--TALSKSKLAINRKPRKRTLVVNFIFANF 60

Query: 61   FTIGFLISLIFFFIVLLRYGVPKPISSHF-RSHATRSHRPRRPIVSENWNREVLSANVDI 120
            F I   +SL+FF + L  +GVP PISS F  S + R  +PR+ I     N     A VDI
Sbjct: 61   FVIALTVSLLFFLLTLFHFGVPGPISSRFLTSRSNRIVKPRKNINRRPLNDSNSGAVVDI 120

Query: 121  TTKELYDKIEFLDVDGGPWKQGWKVTYNGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYE 180
            TTK+LYD+IEFLD DGGPWKQGW+VTY  DEW+ EKLK+FVVPHSHNDPGWKLTV+EYY+
Sbjct: 121  TTKDLYDRIEFLDTDGGPWKQGWRVTYKDDEWEKEKLKIFVVPHSHNDPGWKLTVEEYYQ 180

Query: 181  RQSRRILDTIVEALSKDSRRKFIWEEMSYLEKWWRDASDEKREAFVTLVKNGQLEIVGGG 240
            RQSR ILDTIVE LSKDSRRKFIWEEMSYLE+WWRDAS  K+EA   LVK+GQLEIVGGG
Sbjct: 181  RQSRHILDTIVETLSKDSRRKFIWEEMSYLERWWRDASPNKQEALTKLVKDGQLEIVGGG 240

Query: 241  WVMNDE-----------MAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENM 300
            WVMNDE           +AEGNMWLN+TIGV+PKNSWAIDPFGYS TMAYLLRRMGFENM
Sbjct: 241  WVMNDEANSHYFAIIEQIAEGNMWLNDTIGVIPKNSWAIDPFGYSSTMAYLLRRMGFENM 300

Query: 301  LIQRTHYELKKELALHKNVEFTWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQ 360
            LIQRTHYELKK+LA HKN+E+ WRQSWDA ETTDIFVHMMPFYSYDIPHTCGPEPAICCQ
Sbjct: 301  LIQRTHYELKKDLAQHKNLEYIWRQSWDAMETTDIFVHMMPFYSYDIPHTCGPEPAICCQ 360

Query: 361  FDFARSRGSIYELCPWRLDPVEINRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFR 420
            FDFAR RG  YELCPW   PVE   ENVQERA+ LLDQYRKKS LYRTNTLLIPLGDDFR
Sbjct: 361  FDFARMRGFKYELCPWGKHPVETTLENVQERALKLLDQYRKKSTLYRTNTLLIPLGDDFR 420

Query: 421  YITIDEAEAQFKNYQLLFDHINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGS 480
            YI+IDEAEAQF+NYQ+LFDHINSNPSLNAEAKFGTLEDYFRT+R+EA+++NYS PGEVGS
Sbjct: 421  YISIDEAEAQFRNYQMLFDHINSNPSLNAEAKFGTLEDYFRTVREEADRVNYSRPGEVGS 480

Query: 481  SQISGFPSLSGDFFTYSDRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQ 540
             Q+ GFPSLSGDFFTY+DRQQDYWSGYYVSRPFFKAVDRVLE TLR AE+M++ LLG+C 
Sbjct: 481  GQVVGFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEHTLRGAEIMMSFLLGYCH 540

Query: 541  RSQCEKFPIGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSK 600
            R QCEKFP  F+YKLTAARRNLALFQHHDGVTGTAKD+VV+DYG RMHTSLQDL IFMSK
Sbjct: 541  RIQCEKFPTSFTYKLTAARRNLALFQHHDGVTGTAKDYVVQDYGTRMHTSLQDLQIFMSK 600

Query: 601  GIEVLLGIRH--EKSDHNSLQFETEQMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTR 660
             IEVLLGIRH  EKSD +   FE EQMRSKYD +PVH+ I  +  +  +V+ FNP EQTR
Sbjct: 601  AIEVLLGIRHEKEKSDQSPSFFEAEQMRSKYDARPVHKPIAAREGNSHTVILFNPSEQTR 660

Query: 661  EEVVMIIVNMTDVSVLDSNWTCLESQVSPEFQHDKAKAFTGRHRLHWKISVPALGLQTYY 720
            EEVV ++VN  ++SVLDSNWTC+ SQ+SPE QHD  K FTGRHRL+WK S+PALGL+TY+
Sbjct: 661  EEVVTVVVNRAEISVLDSNWTCVPSQISPEVQHDDTKLFTGRHRLYWKASIPALGLRTYF 720

Query: 721  IANGLFGCEKPKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQ 780
            IANG   CEK  P+KL + S+    PCP PY CSK D DV EI+N+HQ+LVFDV +G L+
Sbjct: 721  IANGNVECEKATPSKLKYASEFDPFPCPPPYSCSKLDNDVTEIRNEHQTLVFDVKNGSLR 780

Query: 781  KVIKKSGSQNVVNEEIAMYSSLGSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVYSYP 840
            K++ ++GS+ VV EEI MYSS  SGAYLF P G+A+ I++  G  V  EG L+QEV+SYP
Sbjct: 781  KIVHRNGSETVVGEEIGMYSSPESGAYLFKPDGEAQPIVQPDGHVVTSEGLLVQEVFSYP 840

Query: 841  KTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKRIYY 900
            KT+WEKSP+S  TRLY+G + +Q  ++E EYHVELLG +FDDRELIVRYKTDVDNK+++Y
Sbjct: 841  KTKWEKSPLSQKTRLYTGGNTLQDQVVEIEYHVELLGNDFDDRELIVRYKTDVDNKKVFY 900

Query: 901  SDLNGFQMSRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLGVASLKDGWLEI 960
            SDLNGFQMSRRE+YDKIPLQGNYYPMPSLAF++GSNGQRFS+HS+QSLGVASLK+GWLEI
Sbjct: 901  SDLNGFQMSRRETYDKIPLQGNYYPMPSLAFIQGSNGQRFSVHSRQSLGVASLKEGWLEI 960

Query: 961  MLDRRLTRDDGRGLGQGVTDNRVMNVVFHTLIESNVSSKLNPVPNHSSLSASLLSHCVGA 1020
            MLDRRL RDDGRGLGQGV DNR M VVFH L ESN+ S+ +P  N +  + SLLSH +GA
Sbjct: 961  MLDRRLVRDDGRGLGQGVMDNRAMTVVFHLLAESNI-SQADPASNTNPRNPSLLSHLIGA 1020

Query: 1021 RLNYPLHTFIAKKPQPSSVQPPP-RSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPED-P 1080
             LNYP++TFIAKKPQ  SV+ P   SF+PLA PLPCDLHIV+FKVPRP KYSQQ  ED P
Sbjct: 1021 HLNYPINTFIAKKPQDISVRVPQYGSFAPLAKPLPCDLHIVNFKVPRPSKYSQQLEEDKP 1080

Query: 1081 RFILIFHRRHWDSSYCKTARS-NCTRFADEPFNMFNMFKSFVVSNARATSLNLLHEDTEM 1140
            RF LI +RR WDS+YC   R  NCT  A+EP N  +MFK    S  + TSLNLL ED E+
Sbjct: 1081 RFALILNRRAWDSAYCHKGRQVNCTSMANEPVNFSDMFKDLAASKVKPTSLNLLQEDMEI 1140

Query: 1141 LGYNEQ----SGDVAHEGQLHIPPMELRAYKLEVQP 1149
            LGY++Q          EG++ I PME+RAYKLE++P
Sbjct: 1141 LGYDDQELPRDSSQPREGRVSISPMEIRAYKLELRP 1171

BLAST of MC08g1343 vs. TAIR 10
Match: AT5G66150.1 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 271.9 bits (694), Expect = 2.3e-72
Identity = 241/893 (26.99%), Postives = 396/893 (44.34%), Query Frame = 0

Query: 148 KLKVFVVPHSHNDPGWKLTVDEYYERQSRRI--------LDTIVEALSKDSRRKFIWEEM 207
           KL V +VPHSH+D GW  TVD+YY   + RI        LD++V++L +D  RKF++ EM
Sbjct: 46  KLNVHLVPHSHDDVGWLKTVDQYYVGSNNRIQNACVRNVLDSVVDSLLRDPNRKFVFAEM 105

Query: 208 SYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDE-----------MAEGNMWLNE 267
           ++  +WW + S E++E    LVK+GQLE V GGW MNDE             +G+ ++ +
Sbjct: 106 AFFTRWWEEQSPERQEQVRRLVKSGQLEFVNGGWAMNDEATCHYIDMIDQTTKGHRFIKQ 165

Query: 268 TIGVVPKNSWAIDPFGYSPTMAYLL-RRMGFENMLIQRTHYELKKELALHKNVEFTWRQS 327
                P+ +W IDPFG+S   AYLL   +G +++   R  Y+ +++    K++E  WR S
Sbjct: 166 QFNTTPRAAWQIDPFGHSSVQAYLLGAELGLDSVHFARIDYQDREKRKAEKSLEVIWRGS 225

Query: 328 WDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSIYEL----CPWRLDPVE 387
              + ++ IF ++  F  Y  P                   G  YE+     P + +P  
Sbjct: 226 KTLDSSSQIFTNIF-FVHYGPP------------------TGFHYEVTDDYVPLQDNP-R 285

Query: 388 INRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAEAQFKNYQLLFDHIN 447
            +  N++E     ++     + + R N ++  +GDDF+Y     AE+ F+    L  ++N
Sbjct: 286 FDGYNIKEAVNDFVNASLVYANVSRGNHVMWTMGDDFQY---QFAESWFRQMDRLIHYVN 345

Query: 448 SNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPG-EVGSSQISG--FPSLSGDFFTYSDR 507
            +  +NA                      YS P   V +  ++   +P  + DFF Y+DR
Sbjct: 346 KDGRVNA---------------------LYSTPSLYVDAKNVANVTWPLKTHDFFPYADR 405

Query: 508 QQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFPIGFSYKLTAAR 567
              YW+GY+ SRP  K   R L     AA   L  L+G              +Y L  A 
Sbjct: 406 AYAYWTGYFTSRPALKRYVRALSGYYMAAR-QLEFLVGKNSGGP-------NTYSLGDA- 465

Query: 568 RNLALFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGIRHEKSDHNSLQ 627
             L + QHHD VTGTAK HV  DY  R+     +    ++  +  L+  +  K       
Sbjct: 466 --LGIAQHHDAVTGTAKQHVTNDYMKRLALGASEAEAVVNSALACLMN-KAPKGGCTKPA 525

Query: 628 FETEQMRSKYDVQPVHRAID-LQIESYQSVVFFNPLEQTREEVVMIIVNMTDVSVLDSNW 687
               Q  S  ++       + L  +    +V +N L   R E++ I VN   +SV DS+ 
Sbjct: 526 IAFSQQCSLMNISYCPSTEETLPGQKSLILVAYNSLGWNRTEIIRIPVNDAGLSVEDSSG 585

Query: 688 TCLESQ------VSPEFQHDKAKAFTG-------RHRLHWKISVPALGLQTYYIANGLFG 747
             L++Q      V+   +    KA+ G       ++ L +K  VP LG  T++I+     
Sbjct: 586 NTLDAQYIPMDNVTSNLRSFYTKAYLGISSLQRPKYWLVFKAKVPPLGWNTFFIS----- 645

Query: 748 CEKPKPAKLNFFSKSTSLPCPTPYVCSKADGDVAEIQNQHQSLVFDVNHGLLQKVI-KKS 807
             K      N    S+ +  P          +  EI   +  +VF  + G L+++   ++
Sbjct: 646 --KASAQGSNNHKHSSVMLSPM--------NNTTEIGPGNLKMVFSSDSGRLERMYNSRT 705

Query: 808 GSQNVVNEEIAMY-SSLG-------SGAYLFNPIGDAKSIIEAGGLTV------------ 867
           G+   V++    Y S++G       SGAY+F P G     + +  +              
Sbjct: 706 GADIKVDQNYFWYASNVGDAKDPQVSGAYIFRPNGSLAYPVSSSKICTVTSAFIGNGNVQ 765

Query: 868 ----IVEGPLMQEVYSYPKTEWEKSP-ISHSTRLYSGDDRIQGHIIEKEYHVELLGK-EF 927
               IV GPL+ EV+       + SP ++   RLY   +  +    E       +GK   
Sbjct: 766 SKLQIVRGPLIDEVHQ------QFSPWVAQVVRLYKEKEHAE---FEFTIGPISVGKGHL 825

Query: 928 DDRELIVRYKTDVDNKRIYYSDLNGFQMSRRESYDKI--------PLQGNYYPMPSLAFM 965
             +E+I R  TD+   + +Y+D NG    +R   ++         P+ GNYYP+    ++
Sbjct: 826 TGKEIITRMVTDMTTAKEFYTDSNGRDFLKRVRDNRTDWHLEVNEPIAGNYYPLNLGMYI 857

BLAST of MC08g1343 vs. TAIR 10
Match: AT5G13980.1 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 267.3 bits (682), Expect = 5.6e-71
Identity = 230/871 (26.41%), Postives = 377/871 (43.28%), Query Frame = 0

Query: 148 KLKVFVVPHSHNDPGWKLTVDEYYERQS--------RRILDTIVEALSKDSRRKFIWEEM 207
           KL V VVPHSH+D GW  TVD+YY   +        + +LD+IV AL  D  RKFI+ E 
Sbjct: 36  KLNVHVVPHSHDDVGWLKTVDQYYVGSNNSIQVACVQNVLDSIVPALLADKNRKFIYVEQ 95

Query: 208 SYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDEMAE-----------GNMWLNE 267
           ++ ++WW + S+E +     L+ +GQLE++ GG  M+DE A            G+ ++  
Sbjct: 96  AFFQRWWNEQSEEIKRIVKELIHSGQLELINGGMCMHDEAAPHYIDMIDQTTLGHRFIIR 155

Query: 268 TIGVVPKNSWAIDPFGYSPTMAYLL-RRMGFENMLIQRTHYELKKELALHKNVEFTWRQS 327
              V P+  W IDPFG+S   AYLL   +GF+++   R  Y+ +++    K +E  WR S
Sbjct: 156 EFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVFFGRIDYQDREKRYKEKTLEVIWRGS 215

Query: 328 WDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSIYEL---CPWRLDPVEI 387
                ++ IF    P  +Y+      P P            G  YE+    P   D  ++
Sbjct: 216 KSLGSSSQIFAGAFP-TNYE------PPPG-----------GFYYEITDDSPVVQDDPDL 275

Query: 388 NRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAEAQFKNYQLLFDHINS 447
              NVQER    +     ++ + R N ++  +G DFRY     A   ++    L  ++N 
Sbjct: 276 FDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRY---QYAHTWYRQMDKLIHYVNL 335

Query: 448 NPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPSLSGDFFTYSDRQQDY 507
           +  +NA   F +    +   +  A +                +P  + D+F Y+DR   Y
Sbjct: 336 DGRVNA---FYSTPSIYTDAKHAANE---------------AWPLKTEDYFPYADRINAY 395

Query: 508 WSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFPIGFSYKLTAARRNLA 567
           W+GY+ SRP  K   RV+     AA   L    G  Q+               +    LA
Sbjct: 396 WTGYFTSRPALKRYVRVMSAYYLAAR-QLEFFKGRSQKGP----------NTDSLADALA 455

Query: 568 LFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGIRHEKSDHNSLQFETE 627
           + QHHD V+GT+K HV  DY  R+         ++     V   + H      +L    +
Sbjct: 456 IAQHHDAVSGTSKQHVANDYAKRLAIG------YVEAESVVATSLAHLTKVDPTLNPTFQ 515

Query: 628 QMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNMTDVSVLDSNWTCLES 687
           Q             ++L       V+ +NPL   R ++V + V   DVSV DS    +ES
Sbjct: 516 QCLLLNISYCPSSEVNLSDGKSLIVLAYNPLGWKRVDIVRLPVVGGDVSVHDSEGHEVES 575

Query: 688 QVSP------EFQHDKAKAFTG-------RHRLHWKISVPALGLQTYYIANGLFGCEKPK 747
           Q+ P        +    +A+ G       ++ L + ++VP LG  TY I+          
Sbjct: 576 QLVPFTDEYVALRKYHVEAYLGQSPTQVPKYWLVFSVTVPPLGFTTYTIST--------- 635

Query: 748 PAKLNFFSKSTSLPCPTPYVCS--KADGDVAEIQNQHQSLVFDVNHG-LLQKVIKKSGSQ 807
                  +K T       YV +  K +  +  I + H  L F  + G  +  V  ++   
Sbjct: 636 -------AKKTDGYSSKSYVSNILKGEQSIINIGHGHLKLSFSTDQGTAINYVNGRTSMT 695

Query: 808 NVVNEEIAMYSSL------------GSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVY 867
             V +  + YS+              SGAY+F P G      E      ++ GPL+ EV+
Sbjct: 696 EPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVPLTVIHGPLVDEVH 755

Query: 868 SYPKTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKR 927
                 W    IS  TR+Y G + ++   I     ++    +   +E++ +  + + + +
Sbjct: 756 Q-QINPW----ISQITRVYKGKEHVEVEFIVGNIPID----DGIGKEVVTQISSSLKSNK 815

Query: 928 IYYSDLNGFQMSRR----ESYDKI----PLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLG 960
            +Y+D +G    +R     S  K+    P+ GNYYP+    +++ S  + FS+   ++ G
Sbjct: 816 TFYTDSSGRDYIKRIRDYRSDWKLDVNQPIAGNYYPINHGIYLQDSK-KEFSVMVDRAFG 824

BLAST of MC08g1343 vs. TAIR 10
Match: AT5G13980.2 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 267.3 bits (682), Expect = 5.6e-71
Identity = 230/871 (26.41%), Postives = 377/871 (43.28%), Query Frame = 0

Query: 148 KLKVFVVPHSHNDPGWKLTVDEYYERQS--------RRILDTIVEALSKDSRRKFIWEEM 207
           KL V VVPHSH+D GW  TVD+YY   +        + +LD+IV AL  D  RKFI+ E 
Sbjct: 36  KLNVHVVPHSHDDVGWLKTVDQYYVGSNNSIQVACVQNVLDSIVPALLADKNRKFIYVEQ 95

Query: 208 SYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDEMAE-----------GNMWLNE 267
           ++ ++WW + S+E +     L+ +GQLE++ GG  M+DE A            G+ ++  
Sbjct: 96  AFFQRWWNEQSEEIKRIVKELIHSGQLELINGGMCMHDEAAPHYIDMIDQTTLGHRFIIR 155

Query: 268 TIGVVPKNSWAIDPFGYSPTMAYLL-RRMGFENMLIQRTHYELKKELALHKNVEFTWRQS 327
              V P+  W IDPFG+S   AYLL   +GF+++   R  Y+ +++    K +E  WR S
Sbjct: 156 EFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVFFGRIDYQDREKRYKEKTLEVIWRGS 215

Query: 328 WDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSIYEL---CPWRLDPVEI 387
                ++ IF    P  +Y+      P P            G  YE+    P   D  ++
Sbjct: 216 KSLGSSSQIFAGAFP-TNYE------PPPG-----------GFYYEITDDSPVVQDDPDL 275

Query: 388 NRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAEAQFKNYQLLFDHINS 447
              NVQER    +     ++ + R N ++  +G DFRY     A   ++    L  ++N 
Sbjct: 276 FDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRY---QYAHTWYRQMDKLIHYVNL 335

Query: 448 NPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPSLSGDFFTYSDRQQDY 507
           +  +NA   F +    +   +  A +                +P  + D+F Y+DR   Y
Sbjct: 336 DGRVNA---FYSTPSIYTDAKHAANE---------------AWPLKTEDYFPYADRINAY 395

Query: 508 WSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFPIGFSYKLTAARRNLA 567
           W+GY+ SRP  K   RV+     AA   L    G  Q+               +    LA
Sbjct: 396 WTGYFTSRPALKRYVRVMSAYYLAAR-QLEFFKGRSQKGP----------NTDSLADALA 455

Query: 568 LFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGIRHEKSDHNSLQFETE 627
           + QHHD V+GT+K HV  DY  R+         ++     V   + H      +L    +
Sbjct: 456 IAQHHDAVSGTSKQHVANDYAKRLAIG------YVEAESVVATSLAHLTKVDPTLNPTFQ 515

Query: 628 QMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNMTDVSVLDSNWTCLES 687
           Q             ++L       V+ +NPL   R ++V + V   DVSV DS    +ES
Sbjct: 516 QCLLLNISYCPSSEVNLSDGKSLIVLAYNPLGWKRVDIVRLPVVGGDVSVHDSEGHEVES 575

Query: 688 QVSP------EFQHDKAKAFTG-------RHRLHWKISVPALGLQTYYIANGLFGCEKPK 747
           Q+ P        +    +A+ G       ++ L + ++VP LG  TY I+          
Sbjct: 576 QLVPFTDEYVALRKYHVEAYLGQSPTQVPKYWLVFSVTVPPLGFTTYTIST--------- 635

Query: 748 PAKLNFFSKSTSLPCPTPYVCS--KADGDVAEIQNQHQSLVFDVNHG-LLQKVIKKSGSQ 807
                  +K T       YV +  K +  +  I + H  L F  + G  +  V  ++   
Sbjct: 636 -------AKKTDGYSSKSYVSNILKGEQSIINIGHGHLKLSFSTDQGTAINYVNGRTSMT 695

Query: 808 NVVNEEIAMYSSL------------GSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVY 867
             V +  + YS+              SGAY+F P G      E      ++ GPL+ EV+
Sbjct: 696 EPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVPLTVIHGPLVDEVH 755

Query: 868 SYPKTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKR 927
                 W    IS  TR+Y G + ++   I     ++    +   +E++ +  + + + +
Sbjct: 756 Q-QINPW----ISQITRVYKGKEHVEVEFIVGNIPID----DGIGKEVVTQISSSLKSNK 815

Query: 928 IYYSDLNGFQMSRR----ESYDKI----PLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLG 960
            +Y+D +G    +R     S  K+    P+ GNYYP+    +++ S  + FS+   ++ G
Sbjct: 816 TFYTDSSGRDYIKRIRDYRSDWKLDVNQPIAGNYYPINHGIYLQDSK-KEFSVMVDRAFG 824

BLAST of MC08g1343 vs. TAIR 10
Match: AT5G13980.3 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 267.3 bits (682), Expect = 5.6e-71
Identity = 230/871 (26.41%), Postives = 377/871 (43.28%), Query Frame = 0

Query: 148 KLKVFVVPHSHNDPGWKLTVDEYYERQS--------RRILDTIVEALSKDSRRKFIWEEM 207
           KL V VVPHSH+D GW  TVD+YY   +        + +LD+IV AL  D  RKFI+ E 
Sbjct: 36  KLNVHVVPHSHDDVGWLKTVDQYYVGSNNSIQVACVQNVLDSIVPALLADKNRKFIYVEQ 95

Query: 208 SYLEKWWRDASDEKREAFVTLVKNGQLEIVGGGWVMNDEMAE-----------GNMWLNE 267
           ++ ++WW + S+E +     L+ +GQLE++ GG  M+DE A            G+ ++  
Sbjct: 96  AFFQRWWNEQSEEIKRIVKELIHSGQLELINGGMCMHDEAAPHYIDMIDQTTLGHRFIIR 155

Query: 268 TIGVVPKNSWAIDPFGYSPTMAYLL-RRMGFENMLIQRTHYELKKELALHKNVEFTWRQS 327
              V P+  W IDPFG+S   AYLL   +GF+++   R  Y+ +++    K +E  WR S
Sbjct: 156 EFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVFFGRIDYQDREKRYKEKTLEVIWRGS 215

Query: 328 WDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSIYEL---CPWRLDPVEI 387
                ++ IF    P  +Y+      P P            G  YE+    P   D  ++
Sbjct: 216 KSLGSSSQIFAGAFP-TNYE------PPPG-----------GFYYEITDDSPVVQDDPDL 275

Query: 388 NRENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYITIDEAEAQFKNYQLLFDHINS 447
              NVQER    +     ++ + R N ++  +G DFRY     A   ++    L  ++N 
Sbjct: 276 FDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRY---QYAHTWYRQMDKLIHYVNL 335

Query: 448 NPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPSLSGDFFTYSDRQQDY 507
           +  +NA   F +    +   +  A +                +P  + D+F Y+DR   Y
Sbjct: 336 DGRVNA---FYSTPSIYTDAKHAANE---------------AWPLKTEDYFPYADRINAY 395

Query: 508 WSGYYVSRPFFKAVDRVLEQTLRAAEMMLALLLGHCQRSQCEKFPIGFSYKLTAARRNLA 567
           W+GY+ SRP  K   RV+     AA   L    G  Q+               +    LA
Sbjct: 396 WTGYFTSRPALKRYVRVMSAYYLAAR-QLEFFKGRSQKGP----------NTDSLADALA 455

Query: 568 LFQHHDGVTGTAKDHVVRDYGLRMHTSLQDLHIFMSKGIEVLLGIRHEKSDHNSLQFETE 627
           + QHHD V+GT+K HV  DY  R+         ++     V   + H      +L    +
Sbjct: 456 IAQHHDAVSGTSKQHVANDYAKRLAIG------YVEAESVVATSLAHLTKVDPTLNPTFQ 515

Query: 628 QMRSKYDVQPVHRAIDLQIESYQSVVFFNPLEQTREEVVMIIVNMTDVSVLDSNWTCLES 687
           Q             ++L       V+ +NPL   R ++V + V   DVSV DS    +ES
Sbjct: 516 QCLLLNISYCPSSEVNLSDGKSLIVLAYNPLGWKRVDIVRLPVVGGDVSVHDSEGHEVES 575

Query: 688 QVSP------EFQHDKAKAFTG-------RHRLHWKISVPALGLQTYYIANGLFGCEKPK 747
           Q+ P        +    +A+ G       ++ L + ++VP LG  TY I+          
Sbjct: 576 QLVPFTDEYVALRKYHVEAYLGQSPTQVPKYWLVFSVTVPPLGFTTYTIST--------- 635

Query: 748 PAKLNFFSKSTSLPCPTPYVCS--KADGDVAEIQNQHQSLVFDVNHG-LLQKVIKKSGSQ 807
                  +K T       YV +  K +  +  I + H  L F  + G  +  V  ++   
Sbjct: 636 -------AKKTDGYSSKSYVSNILKGEQSIINIGHGHLKLSFSTDQGTAINYVNGRTSMT 695

Query: 808 NVVNEEIAMYSSL------------GSGAYLFNPIGDAKSIIEAGGLTVIVEGPLMQEVY 867
             V +  + YS+              SGAY+F P G      E      ++ GPL+ EV+
Sbjct: 696 EPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVPLTVIHGPLVDEVH 755

Query: 868 SYPKTEWEKSPISHSTRLYSGDDRIQGHIIEKEYHVELLGKEFDDRELIVRYKTDVDNKR 927
                 W    IS  TR+Y G + ++   I     ++    +   +E++ +  + + + +
Sbjct: 756 Q-QINPW----ISQITRVYKGKEHVEVEFIVGNIPID----DGIGKEVVTQISSSLKSNK 815

Query: 928 IYYSDLNGFQMSRR----ESYDKI----PLQGNYYPMPSLAFMEGSNGQRFSIHSKQSLG 960
            +Y+D +G    +R     S  K+    P+ GNYYP+    +++ S  + FS+   ++ G
Sbjct: 816 TFYTDSSGRDYIKRIRDYRSDWKLDVNQPIAGNYYPINHGIYLQDSK-KEFSVMVDRAFG 824