MC08g1116 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1116
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein-S-isoprenylcysteine O-methyltransferase
LocationMC08: 9341324 .. 9342746 (-)
RNA-Seq ExpressionMC08g1116
SyntenyMC08g1116
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCGATTATTTGGCATCTACTTTTTAGTGTAATTGCTGTGTTAATTTTGAAACTGCGATCTTCCTGAAGCTGTGAAACCCATATTTTCTTAGTCTTATTATCCCTAATAACATTTAAACGTCTTCCCAATCCCGGTATGTGCACCCAGAAACGTTTACAGGAGAACTTGTTAATGGAATTATGTACCTGCGTAGATCACATTGAAGCTTACAAAAACTTCTTTTTTTCCCGCAAACCTTCCATTAGTACAATTGATTGCACAGGGTATACTAAATCCTTTGAGGATAATCTAGATTTCTATTCATTTATCACATTCATCGCTGATAATTTTATTTAACAAACATGGTTTGTGATAGAAACTCTCTTCTATTATTATTTTTTCTTTGCAGATGTTGCGCTTGAAGGATCTCCAGGATTTTGTTCATAATAATTTCTCGTATTGGTGTTATATAGCTCTGCATCATTTCCAGGTCTGAACTAAAATCTCAGATTACCTGCAAACTTTAGTGCAGTAGCCGAATACATTTCGAATTATTTTTTGGTGAGTGAGAGACTGAGCTGATCCTTTGAGCTGACAAAAAATGATAGAAATATTCAGTTACACAGCATGCAGACAATTGTCTCAGATGATATTTGCAATCATCTTGTTTCACATCTCTGAATATATCTTGGCAGTTGGAATTCATGGGAGGTCAAATGTTACTCTAAATTCCCTTCTGATAAGCAAAAACTATCTCTTGGCAATGGTCTTGTCTTTCTTAGAGTACTTTCTTGAAACTTTGTTGTGTCCCAGTCTTAAGGAATACTGGTGGATTAGTCAGGCAGGCCTTGCAATGGTCGTAATCGGAGAAACTGTACGTAAACTGGCAATTATAACTGCGGGTCGATCATTTACACATCTGATCAAGATCTATCATGAGGATCATCACAAGCTAGTTACTCATGGAGTCTATAGATTTGTTCGTCATCCTGGATACAGCGGTTTCCTCGTGTGGGCGATTGGCACCCAAATCATGCTCTGTAATCCCATCTCCACCATTGCATTTGCAGTTGTGGTTTGGCACTTCTTTGCCAAACGAATTCCGTATGAAGAGTACTTCTTGAGACAGTTTTTTGGGGACGAGTACAAGGAGTATGCGAATCGAGTACCCTCTGGAGTGCCATTCGTGAAATGAAGGCCGAAGATCTGGAGTTGGATTCATCAAGATATTTTTCATGGGTTCTTTGCTCTTTTGAGGTATTTGGAACCTTTAACTGGGAGTAGTTTTGTGGTGTATATTAAAGCTTCTAATTGATGATTTTTGGATGAGGATTCTTTTAATGTAATATTCTTTGTATATGAAGTGAGGAATTGTTGAGCAAGATTTTACCAATGCACAAGTTTGCTAAACTCAGACTTAAACCTTCCACTTAAATCTCAAAG

mRNA sequence

TTCGATTATTTGGCATCTACTTTTTAGTGTAATTGCTGTGTTAATTTTGAAACTGCGATCTTCCTGAAGCTGTGAAACCCATATTTTCTTAGTCTTATTATCCCTAATAACATTTAAACGTCTTCCCAATCCCGGTATGTGCACCCAGAAACGTTTACAGGAGAACTTGTTAATGGAATTATGTACCTGCGTAGATCACATTGAAGCTTACAAAAACTTCTTTTTTTCCCGCAAACCTTCCATTAGTACAATTGATTGCACAGGGTATACTAAATCCTTTGAGGATAATCTAGATTTCTATTCATTTATCACATTCATCGCTGATAATTTTATTTAACAAACATGGTTTGTGATAGAAACTCTCTTCTATTATTATTTTTTCTTTGCAGATGTTGCGCTTGAAGGATCTCCAGGATTTTGTTCATAATAATTTCTCGTATTGGTGTTATATAGCTCTGCATCATTTCCAGGTCTGAACTAAAATCTCAGATTACCTGCAAACTTTAGTGCAGTAGCCGAATACATTTCGAATTATTTTTTGGTGAGTGAGAGACTGAGCTGATCCTTTGAGCTGACAAAAAATGATAGAAATATTCAGTTACACAGCATGCAGACAATTGTCTCAGATGATATTTGCAATCATCTTGTTTCACATCTCTGAATATATCTTGGCAGTTGGAATTCATGGGAGGTCAAATGTTACTCTAAATTCCCTTCTGATAAGCAAAAACTATCTCTTGGCAATGGTCTTGTCTTTCTTAGAGTACTTTCTTGAAACTTTGTTGTGTCCCAGTCTTAAGGAATACTGGTGGATTAGTCAGGCAGGCCTTGCAATGGTCGTAATCGGAGAAACTGTACGTAAACTGGCAATTATAACTGCGGGTCGATCATTTACACATCTGATCAAGATCTATCATGAGGATCATCACAAGCTAGTTACTCATGGAGTCTATAGATTTGTTCGTCATCCTGGATACAGCGGTTTCCTCGTGTGGGCGATTGGCACCCAAATCATGCTCTGTAATCCCATCTCCACCATTGCATTTGCAGTTGTGGTTTGGCACTTCTTTGCCAAACGAATTCCGTATGAAGAGTACTTCTTGAGACAGTTTTTTGGGGACGAGTACAAGGAGTATGCGAATCGAGTACCCTCTGGAGTGCCATTCGTGAAATGAAGGCCGAAGATCTGGAGTTGGATTCATCAAGATATTTTTCATGGGTTCTTTGCTCTTTTGAGGTATTTGGAACCTTTAACTGGGAGTAGTTTTGTGGTGTATATTAAAGCTTCTAATTGATGATTTTTGGATGAGGATTCTTTTAATGTAATATTCTTTGTATATGAAGTGAGGAATTGTTGAGCAAGATTTTACCAATGCACAAGTTTGCTAAACTCAGACTTAAACCTTCCACTTAAATCTCAAAG

Coding sequence (CDS)

ATGATAGAAATATTCAGTTACACAGCATGCAGACAATTGTCTCAGATGATATTTGCAATCATCTTGTTTCACATCTCTGAATATATCTTGGCAGTTGGAATTCATGGGAGGTCAAATGTTACTCTAAATTCCCTTCTGATAAGCAAAAACTATCTCTTGGCAATGGTCTTGTCTTTCTTAGAGTACTTTCTTGAAACTTTGTTGTGTCCCAGTCTTAAGGAATACTGGTGGATTAGTCAGGCAGGCCTTGCAATGGTCGTAATCGGAGAAACTGTACGTAAACTGGCAATTATAACTGCGGGTCGATCATTTACACATCTGATCAAGATCTATCATGAGGATCATCACAAGCTAGTTACTCATGGAGTCTATAGATTTGTTCGTCATCCTGGATACAGCGGTTTCCTCGTGTGGGCGATTGGCACCCAAATCATGCTCTGTAATCCCATCTCCACCATTGCATTTGCAGTTGTGGTTTGGCACTTCTTTGCCAAACGAATTCCGTATGAAGAGTACTTCTTGAGACAGTTTTTTGGGGACGAGTACAAGGAGTATGCGAATCGAGTACCCTCTGGAGTGCCATTCGTGAAATGA

Protein sequence

MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFLEYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVTHGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGDEYKEYANRVPSGVPFVK
Homology
BLAST of MC08g1116 vs. ExPASy Swiss-Prot
Match: Q9FMW9 (Protein-S-isoprenylcysteine O-methyltransferase A OS=Arabidopsis thaliana OX=3702 GN=ICMTA PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 1.7e-76
Identity = 136/196 (69.39%), Postives = 164/196 (83.67%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFS T+ RQLSQM+ ++I FHISEYILA+ IHG SNVTL+SLLI+K+Y LAM+LS L
Sbjct: 1   MTEIFSDTSIRQLSQMLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EY  E +L P LK++WW+S  GL M+++GE +RK AIITAGRSFTHLIKI +E+HH LVT
Sbjct: 61  EYLTEIILFPGLKQHWWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           HGVYR +RHP Y GFL+W++GTQ+MLCNP+S +AFAVVVW FFA+RIPYEEYFL QFFG 
Sbjct: 121 HGVYRLMRHPSYCGFLIWSVGTQVMLCNPVSAVAFAVVVWRFFAQRIPYEEYFLNQFFGV 180

Query: 181 EYKEYANRVPSGVPFV 197
           +Y EYA  V SGVPFV
Sbjct: 181 QYLEYAESVASGVPFV 196

BLAST of MC08g1116 vs. ExPASy Swiss-Prot
Match: Q93W54 (Protein-S-isoprenylcysteine O-methyltransferase B OS=Arabidopsis thaliana OX=3702 GN=ICMTB PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.4e-75
Identity = 137/196 (69.90%), Postives = 162/196 (82.65%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFS T  RQL+QM  AII FH SEYILA+ IHG S VTL+SLLISK+Y LAM++S L
Sbjct: 1   MTEIFSDTGFRQLTQMFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EY  E +  P LK++WWIS  GL M+++GE +RK AIITAGRSFTHLIKI  E+HHKLVT
Sbjct: 61  EYIAEIVFFPGLKQHWWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
            GVY+ +RHP YSGFL+W++GTQ+MLCNPIS IAFAVVVW FFA+RIPYEE++L+QFFG 
Sbjct: 121 EGVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGR 180

Query: 181 EYKEYANRVPSGVPFV 197
           +Y EYA RVPSGVPFV
Sbjct: 181 QYVEYAQRVPSGVPFV 196

BLAST of MC08g1116 vs. ExPASy Swiss-Prot
Match: A2XX73 (Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. indica OX=39946 GN=ICMT PE=3 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 1.8e-70
Identity = 119/179 (66.48%), Postives = 152/179 (84.92%), Query Frame = 0

Query: 19  AIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFLEYFLETLLCPSLKEYWWI 78
           A+++FH SEY+LA   HGR NVT  SLLISK Y+LAM  + LE+  E LL P LKEYW++
Sbjct: 13  ALVIFHGSEYVLAAAFHGRRNVTATSLLISKQYVLAMSFAMLEHLTEALLFPELKEYWFV 72

Query: 79  SQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVTHGVYRFVRHPGYSGFLVW 138
           S  GL MV+IGE +RKLA++TAGRSFTH+I+I++ED HKL+THGVYR +RHPGYSGFL+W
Sbjct: 73  SYVGLVMVIIGEVIRKLAVVTAGRSFTHVIRIHYEDQHKLITHGVYRLMRHPGYSGFLIW 132

Query: 139 AIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGDEYKEYANRVPSGVPFVK 198
           A+GTQ+MLCNP+ST+AF +V+W FF+KRIPYEE+FLRQFFG EY+EYA +V SG+PF++
Sbjct: 133 AVGTQVMLCNPLSTVAFTLVLWRFFSKRIPYEEFFLRQFFGREYEEYAQKVHSGLPFIE 191

BLAST of MC08g1116 vs. ExPASy Swiss-Prot
Match: Q7XSR9 (Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=ICMT PE=3 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 1.8e-70
Identity = 119/179 (66.48%), Postives = 152/179 (84.92%), Query Frame = 0

Query: 19  AIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFLEYFLETLLCPSLKEYWWI 78
           A+++FH SEY+LA   HGR NVT  SLLISK Y+LAM  + LE+  E LL P LKEYW++
Sbjct: 13  ALVIFHGSEYVLAAAFHGRRNVTATSLLISKQYVLAMSFAMLEHLTEALLFPELKEYWFV 72

Query: 79  SQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVTHGVYRFVRHPGYSGFLVW 138
           S  GL MV+IGE +RKLA++TAGRSFTH+I+I++ED HKL+THGVYR +RHPGYSGFL+W
Sbjct: 73  SYVGLVMVIIGEVIRKLAVVTAGRSFTHVIRIHYEDQHKLITHGVYRLMRHPGYSGFLIW 132

Query: 139 AIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGDEYKEYANRVPSGVPFVK 198
           A+GTQ+MLCNP+ST+AF +V+W FF+KRIPYEE+FLRQFFG EY+EYA +V SG+PF++
Sbjct: 133 AVGTQVMLCNPLSTVAFTLVLWRFFSKRIPYEEFFLRQFFGREYEEYAQKVHSGLPFIE 191

BLAST of MC08g1116 vs. ExPASy Swiss-Prot
Match: Q9WVM4 (Protein-S-isoprenylcysteine O-methyltransferase OS=Rattus norvegicus OX=10116 GN=Icmt PE=2 SV=2)

HSP 1 Score: 171.8 bits (434), Expect = 7.8e-42
Identity = 85/195 (43.59%), Postives = 125/195 (64.10%), Query Frame = 0

Query: 5   FSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLI--SKNYLLAMVLSFLEY 64
           FS ++       + ++ LFH SEY L   ++   +++L+S L+  S  Y +A + S++E+
Sbjct: 85  FSQSSWNHFGWYVCSLSLFHYSEY-LVTAVNNPKSLSLDSFLLNHSLEYTVAALSSWIEF 144

Query: 65  FLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVTHG 124
            LE +  P LK+  W+S AGL MV+ GE +RK+A+ TAG +F H+++    D H LVT G
Sbjct: 145 TLENIFWPELKQITWLSAAGLLMVIFGECLRKVAMFTAGSNFNHVVQSEKSDTHTLVTSG 204

Query: 125 VYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGDEY 184
           VY + RHP Y G+  W+IGTQ+MLCNPI  + +A+ VW FF  R   EE  L  FFG+EY
Sbjct: 205 VYAWCRHPSYVGWFYWSIGTQVMLCNPICGVVYALTVWRFFRDRTEEEEISLIHFFGEEY 264

Query: 185 KEYANRVPSGVPFVK 198
            +Y  RVP+G+PF+K
Sbjct: 265 LDYKKRVPTGLPFIK 278

BLAST of MC08g1116 vs. NCBI nr
Match: XP_022148265.1 (protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148266.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148267.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148268.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148269.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148270.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148271.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148272.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148273.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP_022148274.1 protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia])

HSP 1 Score: 391 bits (1004), Expect = 4.69e-137
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL
Sbjct: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT
Sbjct: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD
Sbjct: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180

Query: 181 EYKEYANRVPSGVPFVK 197
           EYKEYANRVPSGVPFVK
Sbjct: 181 EYKEYANRVPSGVPFVK 197

BLAST of MC08g1116 vs. NCBI nr
Match: XP_022156109.1 (protein-S-isoprenylcysteine O-methyltransferase A-like isoform X2 [Momordica charantia])

HSP 1 Score: 362 bits (928), Expect = 1.82e-125
Identity = 178/197 (90.36%), Postives = 188/197 (95.43%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFSYTACRQLSQMIFAII FHISEYILAVGIHGRSNVTLNSLLISKNY+LAMVLSFL
Sbjct: 1   MTEIFSYTACRQLSQMIFAIIFFHISEYILAVGIHGRSNVTLNSLLISKNYILAMVLSFL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EYFLE +LCP LKEYWW+SQ GLAMVVIGE VRKLAIITAGRSFTHLIKIYHEDHHKLVT
Sbjct: 61  EYFLEIMLCPGLKEYWWVSQTGLAMVVIGEIVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           +GVYRFVRHPGYSGFLVWA+GTQIMLCNP+ST+AFAVVVWHFFA+RIPYEEY+LR+FFG 
Sbjct: 121 YGVYRFVRHPGYSGFLVWAVGTQIMLCNPVSTVAFAVVVWHFFAERIPYEEYYLRRFFGH 180

Query: 181 EYKEYANRVPSGVPFVK 197
           EY EYANRV SGVPFVK
Sbjct: 181 EYVEYANRVSSGVPFVK 197

BLAST of MC08g1116 vs. NCBI nr
Match: XP_023531976.1 (protein-S-isoprenylcysteine O-methyltransferase A-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360 bits (925), Expect = 5.21e-125
Identity = 176/197 (89.34%), Postives = 186/197 (94.42%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFSYTACRQLSQMIFAII FH SEYILAVGIHGR NVTL SLLISKNYLLAM+LSFL
Sbjct: 1   MTEIFSYTACRQLSQMIFAIIFFHGSEYILAVGIHGRQNVTLKSLLISKNYLLAMILSFL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EYFLE LLCPSLKEYWW+S  GLAMVV+GE VRKLAIITAGRSFTHLIK+YHEDHHKLVT
Sbjct: 61  EYFLEVLLCPSLKEYWWVSHIGLAMVVVGEIVRKLAIITAGRSFTHLIKVYHEDHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           HGVYRFVRHPGY+GFLVWA+GTQIMLCNPIST+AFA+VVWHFFA+RIPYEEYFLRQFFG 
Sbjct: 121 HGVYRFVRHPGYNGFLVWAVGTQIMLCNPISTVAFAIVVWHFFAERIPYEEYFLRQFFGH 180

Query: 181 EYKEYANRVPSGVPFVK 197
           EY+EYA RVPSGVPFVK
Sbjct: 181 EYEEYARRVPSGVPFVK 197

BLAST of MC08g1116 vs. NCBI nr
Match: XP_022156108.1 (protein-S-isoprenylcysteine O-methyltransferase A-like isoform X1 [Momordica charantia])

HSP 1 Score: 361 bits (926), Expect = 8.88e-125
Identity = 177/195 (90.77%), Postives = 187/195 (95.90%), Query Frame = 0

Query: 3   EIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFLEY 62
           EIFSYTACRQLSQMIFAII FHISEYILAVGIHGRSNVTLNSLLISKNY+LAMVLSFLEY
Sbjct: 28  EIFSYTACRQLSQMIFAIIFFHISEYILAVGIHGRSNVTLNSLLISKNYILAMVLSFLEY 87

Query: 63  FLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVTHG 122
           FLE +LCP LKEYWW+SQ GLAMVVIGE VRKLAIITAGRSFTHLIKIYHEDHHKLVT+G
Sbjct: 88  FLEIMLCPGLKEYWWVSQTGLAMVVIGEIVRKLAIITAGRSFTHLIKIYHEDHHKLVTYG 147

Query: 123 VYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGDEY 182
           VYRFVRHPGYSGFLVWA+GTQIMLCNP+ST+AFAVVVWHFFA+RIPYEEY+LR+FFG EY
Sbjct: 148 VYRFVRHPGYSGFLVWAVGTQIMLCNPVSTVAFAVVVWHFFAERIPYEEYYLRRFFGHEY 207

Query: 183 KEYANRVPSGVPFVK 197
            EYANRV SGVPFVK
Sbjct: 208 VEYANRVSSGVPFVK 222

BLAST of MC08g1116 vs. NCBI nr
Match: XP_023531975.1 (protein-S-isoprenylcysteine O-methyltransferase A-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360 bits (923), Expect = 2.21e-124
Identity = 175/195 (89.74%), Postives = 185/195 (94.87%), Query Frame = 0

Query: 3   EIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFLEY 62
           EIFSYTACRQLSQMIFAII FH SEYILAVGIHGR NVTL SLLISKNYLLAM+LSFLEY
Sbjct: 24  EIFSYTACRQLSQMIFAIIFFHGSEYILAVGIHGRQNVTLKSLLISKNYLLAMILSFLEY 83

Query: 63  FLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVTHG 122
           FLE LLCPSLKEYWW+S  GLAMVV+GE VRKLAIITAGRSFTHLIK+YHEDHHKLVTHG
Sbjct: 84  FLEVLLCPSLKEYWWVSHIGLAMVVVGEIVRKLAIITAGRSFTHLIKVYHEDHHKLVTHG 143

Query: 123 VYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGDEY 182
           VYRFVRHPGY+GFLVWA+GTQIMLCNPIST+AFA+VVWHFFA+RIPYEEYFLRQFFG EY
Sbjct: 144 VYRFVRHPGYNGFLVWAVGTQIMLCNPISTVAFAIVVWHFFAERIPYEEYFLRQFFGHEY 203

Query: 183 KEYANRVPSGVPFVK 197
           +EYA RVPSGVPFVK
Sbjct: 204 EEYARRVPSGVPFVK 218

BLAST of MC08g1116 vs. ExPASy TrEMBL
Match: A0A6J1D2G4 (Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111016968 PE=3 SV=1)

HSP 1 Score: 391 bits (1004), Expect = 2.27e-137
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL
Sbjct: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT
Sbjct: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD
Sbjct: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180

Query: 181 EYKEYANRVPSGVPFVK 197
           EYKEYANRVPSGVPFVK
Sbjct: 181 EYKEYANRVPSGVPFVK 197

BLAST of MC08g1116 vs. ExPASy TrEMBL
Match: A0A6J1DSC7 (Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111023074 PE=3 SV=1)

HSP 1 Score: 362 bits (928), Expect = 8.79e-126
Identity = 178/197 (90.36%), Postives = 188/197 (95.43%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFSYTACRQLSQMIFAII FHISEYILAVGIHGRSNVTLNSLLISKNY+LAMVLSFL
Sbjct: 1   MTEIFSYTACRQLSQMIFAIIFFHISEYILAVGIHGRSNVTLNSLLISKNYILAMVLSFL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EYFLE +LCP LKEYWW+SQ GLAMVVIGE VRKLAIITAGRSFTHLIKIYHEDHHKLVT
Sbjct: 61  EYFLEIMLCPGLKEYWWVSQTGLAMVVIGEIVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           +GVYRFVRHPGYSGFLVWA+GTQIMLCNP+ST+AFAVVVWHFFA+RIPYEEY+LR+FFG 
Sbjct: 121 YGVYRFVRHPGYSGFLVWAVGTQIMLCNPVSTVAFAVVVWHFFAERIPYEEYYLRRFFGH 180

Query: 181 EYKEYANRVPSGVPFVK 197
           EY EYANRV SGVPFVK
Sbjct: 181 EYVEYANRVSSGVPFVK 197

BLAST of MC08g1116 vs. ExPASy TrEMBL
Match: A0A6J1DPQ1 (Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111023074 PE=3 SV=1)

HSP 1 Score: 361 bits (926), Expect = 4.30e-125
Identity = 177/195 (90.77%), Postives = 187/195 (95.90%), Query Frame = 0

Query: 3   EIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFLEY 62
           EIFSYTACRQLSQMIFAII FHISEYILAVGIHGRSNVTLNSLLISKNY+LAMVLSFLEY
Sbjct: 28  EIFSYTACRQLSQMIFAIIFFHISEYILAVGIHGRSNVTLNSLLISKNYILAMVLSFLEY 87

Query: 63  FLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVTHG 122
           FLE +LCP LKEYWW+SQ GLAMVVIGE VRKLAIITAGRSFTHLIKIYHEDHHKLVT+G
Sbjct: 88  FLEIMLCPGLKEYWWVSQTGLAMVVIGEIVRKLAIITAGRSFTHLIKIYHEDHHKLVTYG 147

Query: 123 VYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGDEY 182
           VYRFVRHPGYSGFLVWA+GTQIMLCNP+ST+AFAVVVWHFFA+RIPYEEY+LR+FFG EY
Sbjct: 148 VYRFVRHPGYSGFLVWAVGTQIMLCNPVSTVAFAVVVWHFFAERIPYEEYYLRRFFGHEY 207

Query: 183 KEYANRVPSGVPFVK 197
            EYANRV SGVPFVK
Sbjct: 208 VEYANRVSSGVPFVK 222

BLAST of MC08g1116 vs. ExPASy TrEMBL
Match: A0A6J1JNL6 (Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486321 PE=3 SV=1)

HSP 1 Score: 358 bits (919), Expect = 2.07e-124
Identity = 174/197 (88.32%), Postives = 186/197 (94.42%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFSYTACRQLSQMIFAII FH SEYILAVGIHGR NVTL SLLISKNYLLAM+LSFL
Sbjct: 1   MTEIFSYTACRQLSQMIFAIIFFHGSEYILAVGIHGRQNVTLKSLLISKNYLLAMILSFL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EYFLE L+CP+LKEYWW+S  GLAMVV+GE VRKLAIITAGRSFTHLIK+YHEDHHKLVT
Sbjct: 61  EYFLEVLVCPNLKEYWWVSHIGLAMVVVGEIVRKLAIITAGRSFTHLIKVYHEDHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           HGVYRFVRHPGY+GFLVWA+GTQIMLCNPIST+AFA+VVWHFFA+RIPYEEYFLRQFFG 
Sbjct: 121 HGVYRFVRHPGYNGFLVWAVGTQIMLCNPISTVAFAIVVWHFFAERIPYEEYFLRQFFGH 180

Query: 181 EYKEYANRVPSGVPFVK 197
           EY+EYA RVPSGVPFVK
Sbjct: 181 EYEEYARRVPSGVPFVK 197

BLAST of MC08g1116 vs. ExPASy TrEMBL
Match: A0A6J1EPD2 (Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111435324 PE=3 SV=1)

HSP 1 Score: 357 bits (916), Expect = 5.94e-124
Identity = 173/197 (87.82%), Postives = 185/197 (93.91%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFSYTACRQLSQMIFAII FH SEYILAVGIHGR NVTL SLLISKNYLLAM+LSF+
Sbjct: 1   MTEIFSYTACRQLSQMIFAIIFFHGSEYILAVGIHGRQNVTLKSLLISKNYLLAMILSFI 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EYFLE  LCP+LKEYWW+S  GLAMVV+GE VRKLAIITAGRSFTHLIK+YHEDHHKLVT
Sbjct: 61  EYFLEVFLCPTLKEYWWVSHIGLAMVVVGEIVRKLAIITAGRSFTHLIKVYHEDHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           HGVYRFVRHPGY+GFLVWA+GTQIMLCNPIST+AFA+VVWHFFA+RIPYEEYFLRQFFG 
Sbjct: 121 HGVYRFVRHPGYNGFLVWAVGTQIMLCNPISTVAFAIVVWHFFAERIPYEEYFLRQFFGH 180

Query: 181 EYKEYANRVPSGVPFVK 197
           EY+EYA RVPSGVPFVK
Sbjct: 181 EYEEYARRVPSGVPFVK 197

BLAST of MC08g1116 vs. TAIR 10
Match: AT5G23320.1 (homolog of yeast STE14 A )

HSP 1 Score: 287.0 bits (733), Expect = 1.2e-77
Identity = 136/196 (69.39%), Postives = 164/196 (83.67%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFS T+ RQLSQM+ ++I FHISEYILA+ IHG SNVTL+SLLI+K+Y LAM+LS L
Sbjct: 1   MTEIFSDTSIRQLSQMLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EY  E +L P LK++WW+S  GL M+++GE +RK AIITAGRSFTHLIKI +E+HH LVT
Sbjct: 61  EYLTEIILFPGLKQHWWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
           HGVYR +RHP Y GFL+W++GTQ+MLCNP+S +AFAVVVW FFA+RIPYEEYFL QFFG 
Sbjct: 121 HGVYRLMRHPSYCGFLIWSVGTQVMLCNPVSAVAFAVVVWRFFAQRIPYEEYFLNQFFGV 180

Query: 181 EYKEYANRVPSGVPFV 197
           +Y EYA  V SGVPFV
Sbjct: 181 QYLEYAESVASGVPFV 196

BLAST of MC08g1116 vs. TAIR 10
Match: AT5G08335.1 (Isoprenylcysteine carboxyl methyltransferase (ICMT) family )

HSP 1 Score: 283.9 bits (725), Expect = 1.0e-76
Identity = 137/196 (69.90%), Postives = 162/196 (82.65%), Query Frame = 0

Query: 1   MIEIFSYTACRQLSQMIFAIILFHISEYILAVGIHGRSNVTLNSLLISKNYLLAMVLSFL 60
           M EIFS T  RQL+QM  AII FH SEYILA+ IHG S VTL+SLLISK+Y LAM++S L
Sbjct: 1   MTEIFSDTGFRQLTQMFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVL 60

Query: 61  EYFLETLLCPSLKEYWWISQAGLAMVVIGETVRKLAIITAGRSFTHLIKIYHEDHHKLVT 120
           EY  E +  P LK++WWIS  GL M+++GE +RK AIITAGRSFTHLIKI  E+HHKLVT
Sbjct: 61  EYIAEIVFFPGLKQHWWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVT 120

Query: 121 HGVYRFVRHPGYSGFLVWAIGTQIMLCNPISTIAFAVVVWHFFAKRIPYEEYFLRQFFGD 180
            GVY+ +RHP YSGFL+W++GTQ+MLCNPIS IAFAVVVW FFA+RIPYEE++L+QFFG 
Sbjct: 121 EGVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGR 180

Query: 181 EYKEYANRVPSGVPFV 197
           +Y EYA RVPSGVPFV
Sbjct: 181 QYVEYAQRVPSGVPFV 196

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMW91.7e-7669.39Protein-S-isoprenylcysteine O-methyltransferase A OS=Arabidopsis thaliana OX=370... [more]
Q93W541.4e-7569.90Protein-S-isoprenylcysteine O-methyltransferase B OS=Arabidopsis thaliana OX=370... [more]
A2XX731.8e-7066.48Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. ... [more]
Q7XSR91.8e-7066.48Probable protein-S-isoprenylcysteine O-methyltransferase OS=Oryza sativa subsp. ... [more]
Q9WVM47.8e-4243.59Protein-S-isoprenylcysteine O-methyltransferase OS=Rattus norvegicus OX=10116 GN... [more]
Match NameE-valueIdentityDescription
XP_022148265.14.69e-137100.00protein-S-isoprenylcysteine O-methyltransferase A-like [Momordica charantia] >XP... [more]
XP_022156109.11.82e-12590.36protein-S-isoprenylcysteine O-methyltransferase A-like isoform X2 [Momordica cha... [more]
XP_023531976.15.21e-12589.34protein-S-isoprenylcysteine O-methyltransferase A-like isoform X2 [Cucurbita pep... [more]
XP_022156108.18.88e-12590.77protein-S-isoprenylcysteine O-methyltransferase A-like isoform X1 [Momordica cha... [more]
XP_023531975.12.21e-12489.74protein-S-isoprenylcysteine O-methyltransferase A-like isoform X1 [Cucurbita pep... [more]
Match NameE-valueIdentityDescription
A0A6J1D2G42.27e-137100.00Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 G... [more]
A0A6J1DSC78.79e-12690.36Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 G... [more]
A0A6J1DPQ14.30e-12590.77Protein-S-isoprenylcysteine O-methyltransferase OS=Momordica charantia OX=3673 G... [more]
A0A6J1JNL62.07e-12488.32Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EPD25.94e-12487.82Protein-S-isoprenylcysteine O-methyltransferase OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G23320.11.2e-7769.39homolog of yeast STE14 A [more]
AT5G08335.11.0e-7669.90Isoprenylcysteine carboxyl methyltransferase (ICMT) family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007269Isoprenylcysteine carboxyl methyltransferasePFAMPF04140ICMTcoord: 83..174
e-value: 4.3E-28
score: 97.6
NoneNo IPR availableGENE3D1.20.120.1630coord: 9..196
e-value: 1.2E-38
score: 134.8
NoneNo IPR availablePANTHERPTHR12714PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASEcoord: 12..197
NoneNo IPR availablePANTHERPTHR12714:SF19PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASEcoord: 12..197
IPR025770Protein-S-isoprenylcysteine O-methyltransferasePROSITEPS51564SAM_ICMTcoord: 6..197
score: 25.524727

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1116.1MC08g1116.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006481 C-terminal protein methylation
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005783 endoplasmic reticulum
molecular_function GO:0004671 protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity