MC08g0959 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0959
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	dTMP kinase
Location	MC08: 7676309 .. 7680365 (-)
RNA-Seq Expression	MC08g0959
Synteny	MC08g0959

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACCCTCCAATTTGAAACATCCTCACGAAAAAAAGACATCCCACGCGCGCACAGCCACTCACCGTCCATTCTTCCGGCGACTGAGCGACGGCGGCGGGTTCTAAACTCAGACAACTGAGGCGGCGAACTCCAGCGGGCAAGCAACGGCGACGAGCTCCAACGAACTGTTCAAGTACGTTTTTACTCATAACTTAACCGAACACTATTTTAGTTTTTTCAACTCTTTGTTCTTCGTTTTAATTTTAATTTTGTTATTCTTCTGATCGTTTTCATTTTTGTAACTTGTGTTTTCTTCATCATAGTTTGATTTCTTGATTCGATATTTGATTTTGGCATTGCAAAATATTGCAAAAAAGAAAAAAAAAGGAAGTAAAATGGTGATTTGCTTTTCACATTTCTGTTTATTTGGAACATGTTATTCAATGGATTAGAATTTGTATTTTCATAGTTATTTTTTTTTTTAGTTTGGTATTACGATAAGTTGCAAAACAAAAAAGTCAGAATGTTAAAAGTCCAGGCTTCTTCTTTTCCTTTTTTTTTGTGTATTTCTTAGACCATTTCATGCATTTGAAATATATTAGTGCTATTCAATGAGCCTGAACTTCTGCTTTCATGGTTTGTTAGTTGATTTTGACATTGCAATATATTGCAAGCAAAGCAATCTCATGATCGAGGATTAATTTTGGCTCTTAATGCTGTTTCGGTTTCATCTGTAAGTTACTACCTCCTTCCTCTTCCGGTTTTAAGTATTCTTGGGTTTTGAGAGAACGTGTAACGAAGGTTCCTGCTGTCTTTGCAAACTGACAAACTTCATGTTTAAATTTTTCGGAAACTAAAGAATCAAGGTTGTGATGGTCCTTTCGCTTTGGTGTTTTCTTCACCACGGTCGGAGTGTTGTCAGTACTTTAGCTTTCACGATTTACCTTCGAATTTCATACGAGCAAACCAGATATTGATGTAATAATTAACTGGTCTATTACGAATGGGTGAGAGAATGTTTTGTACTGTAAACATAAAGAGAAAAAGGAACGTAAAGTCTCTTGCTCATGAAATCAGGGTCAGAAGAGAAGCAACCCACTATTATAACAACGGGAAGTGTATTCAATATTCATAGTTCCACTCTAATTTCCTAACGCTGGATTAGCTCTATTTAGTGCCGTCTGCATCTGTGACATTTACGAGTTATATTTTTTGGGAGTCAATTGACTTGGGCGTAAAGCTTTTGAGCATACACTTTGACACAGTTTCAAGGTCAGGACATGATCCATGCTCGCTTCCACATTCCATAAGCCATTGTGAGATTAGTTTCTTCTTTCACCTGTAATTTGTGATGTTTATTTTTTGGGAAATTTATTTTTATTTGTTTAGGTTTGTGGTGGGCGGGTTCGGTGGACATTTGAAATTTGTTTTAAAGGTGCAGTCCCCTTGTTTTTTCTAACAAATATGCATCACACAGTTTCTGCTTCAACCACGAGAATCTATGAAATTGTCTGGAATGATTGATATGGAAGACTTCTCTGATGTTCATTTTTCCCTTTTAGGAGTTTTGGAGCAGTAACAGCTAGAAGATCCTCAAGTGCCCTACTGAAAATTCCTTCTAATAATTTAATCCGGAAGATCCAAATGGGAACTAATCATAACTCTGTGCTAAGAGGTTGCAGCAGTAATTCTAGAGGAGCCCTGGTGGTTCTAGAAGGTTTGGACCGCTGTGGTAAGACTACACAGTCCAGTAGATTAGTCCAATACTTAGAAGGACTTGGCCATTCTGCTGAACTTTTGCGGTTTCCTGATAGAACTACAAGTGTTGGACAAATGATATCTTCCTATCTTTCAAACGAATCACAGCTGGATGACCACACAATACATCTTCTCTTCAGTGCCAATCGATGGGAGAAGAGGTTTGTTAACTTTTCTTTATACCACTTTCAGTTTAGGCCCTTAGATTGATAAACTTTCAGTACCTGATGAGTGATGGGGCTTCCAATGTTGTTATTAAGTGGCAAGTTTTTCTTAAAATGAAAAATGGTGAGACCTGGTTTTAGAAGTTCCAAGCAATGGCAGTTGAGAACTTGGTGGACCGTGGACTACAACCATTTTCGTGCATGTCGTACGCTGAGGGAAAACCTGTATATTATATCAACATTTATTTTAGATGAAGAATAGTGTATATGCACCCCAAGAGTTTGTGATTTCGGTTTAATTACTGTAATGGCTGTCAGATCGTTGATGGAAACAAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCAGCTGCCAAAGGACTTGGTATCGAATGGTGCAAGGTATAAATACTAATTAGTAATTTGGCTGAATCCTTTGTTTATGCATGTTTATGTTTTTTTTTTCTTTTTTCTTTTTATTATAAATTTGGGGTTTTTAAATGCTCTGATGTTATGTTTGTCCTTTTCAGGCACCGGAGGTTGGCTTGCTAGCTCCGGACTTGGTACTGTACCTAGACATATCTCCAGAGGTTAGTTAAATTAGTATATCATTCTTTTTGGGGAAGAAATTTAAATGCATTTATTGGGATGGGCTTACCATAATTTACCTTTTTCTAACTTGTAAATGTAATGAAGCTATGATGTAAATTTATTGACTATCTTATATTGAAAATAAATGATTTTGTACTAATTTTATGATACAATTTAATTCTGAAATTTTGTCATATTTTTATTTTTAATTCAAGCTAAAATTTTTTTTGCATATCACCCATACTGATTCAAGCCACTAAATCATATCTTTCACGGATCTTTCTAGTCATAGCTGATATATGTTACATTTGTATTGGGTTTTTTATGGAACTCTATCAATTGTATTGGTAGCATAGAGTAAGCAATTGCTCAAGTAGAGCAGGTCTTATAGAGACTTCTTTTGAAGTTTTATTATGTAATTGATGGCAATGGACTTTTGAGAATAACAATTTATAATCAAGGGAATAATGTCCTTTGTGCAAACTATGATATCAATTTATAACCTAGGAGCTGGTTAGATTTAATACCTCTTTTTGAGTTTCAGTGTCTTAGGCTTTTTGTAATTACCACTTAGGTGTCATTCTTTTAGATTGAGGTCCATTCTTAATGGTAGTTCCCTTTTGTCGGGGTTGTTTTTTGTATGCCCCTTGTATATTCTTTCATTTTCTGTTAATGAAAGCTAGGTTTTTGTTTTGACTTTGATCTGCAACCCCTGGTAACTTCATAAATTCTCAATTTTAAAACTTCCAATTTGTATTATGATTGATCAATGATAAAGAGTTCTGAATGATCAGAAAGCTGTAGAAAGAGGAGGTTATGGATGCGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTACCAAGTTTTGCATGATTCCTCTTGGAAGGTAACTTGTTTTTCATTTGTGTCTTGGAAATATTGACACTCCAAGTAAATCAAAACAATAATATTCAATGAAGTTATGCACGAGGAATCCAATGTTTTTTCACGTTCATCTTAGGTGGAAGCCTGTTTTTCATATTAAAAAAAAAAAGATTGACTGACTGCATTTTGGACTTTCCTTGATCTCATTGCAGATAATTGATGCTTGCCAACCCATGGAAGATATTGAGAAGCAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGACGCCACAACGGGGAACCGCTTTCCGAGCTTTGGTTAAGTTGATGCTTTCTTTTGAAGCCTGAAGATCTTTTTCCCGGGTGTATTTATATTCAAAATGTTGAGATTTTGGGGGCTTGTCCTGTGTGTTTTTATATTCTTTTTACATCTATATAATTTTAGTTTGCTTTTCTTTATCACAAATTTTTAGCGTTCAAGAATTATAGGCATTATAAATCAAAGAAAAGTCTCTGTCATTACACTTCTATCAGATCTTCCCCCCCCCCCCCCCCCAATTGGGCTACACCAATATTTGAGAACACTGAAAGACTTATAATTTTTTAAATTATTAATTAGAGCAAGACTAATTCATTCTAATCTTGAAAATTTTGAGAG

mRNA sequence

AAACCCTCCAATTTGAAACATCCTCACGAAAAAAAGACATCCCACGCGCGCACAGCCACTCACCGTCCATTCTTCCGGCGACTGAGCGACGGCGGCGGGTTCTAAACTCAGACAACTGAGGCGGCGAACTCCAGCGGGCAAGCAACGGCGACGAGCTCCAACGAACTGTTCAATTGATTTTGACATTGCAATATATTGCAAGCAAAGCAATCTCATGATCGAGGATTAATTTTGGCTCTTAATGCTGTTTCGGTTTCATCTGAGTTTTGGAGCAGTAACAGCTAGAAGATCCTCAAGTGCCCTACTGAAAATTCCTTCTAATAATTTAATCCGGAAGATCCAAATGGGAACTAATCATAACTCTGTGCTAAGAGGTTGCAGCAGTAATTCTAGAGGAGCCCTGGTGGTTCTAGAAGGTTTGGACCGCTGTGGTAAGACTACACAGTCCAGTAGATTAGTCCAATACTTAGAAGGACTTGGCCATTCTGCTGAACTTTTGCGGTTTCCTGATAGAACTACAAGTGTTGGACAAATGATATCTTCCTATCTTTCAAACGAATCACAGCTGGATGACCACACAATACATCTTCTCTTCAGTGCCAATCGATGGGAGAAGAGATCGTTGATGGAAACAAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCAGCTGCCAAAGGACTTGGTATCGAATGGTGCAAGGCACCGGAGGTTGGCTTGCTAGCTCCGGACTTGGTACTGTACCTAGACATATCTCCAGAGAAAGCTGTAGAAAGAGGAGGTTATGGATGCGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTACCAAGTTTTGCATGATTCCTCTTGGAAGATAATTGATGCTTGCCAACCCATGGAAGATATTGAGAAGCAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGACGCCACAACGGGGAACCGCTTTCCGAGCTTTGGTTAAGTTGATGCTTTCTTTTGAAGCCTGAAGATCTTTTTCCCGGGTGTATTTATATTCAAAATGTTGAGATTTTGGGGGCTTGTCCTGTGTGTTTTTATATTCTTTTTACATCTATATAATTTTAGTTTGCTTTTCTTTATCACAAATTTTTAGCGTTCAAGAATTATAGGCATTATAAATCAAAGAAAAGTCTCTGTCATTACACTTCTATCAGATCTTCCCCCCCCCCCCCCCCCAATTGGGCTACACCAATATTTGAGAACACTGAAAGACTTATAATTTTTTAAATTATTAATTAGAGCAAGACTAATTCATTCTAATCTTGAAAATTTTGAGAG

Coding sequence (CDS)

ATGCTGTTTCGGTTTCATCTGAGTTTTGGAGCAGTAACAGCTAGAAGATCCTCAAGTGCCCTACTGAAAATTCCTTCTAATAATTTAATCCGGAAGATCCAAATGGGAACTAATCATAACTCTGTGCTAAGAGGTTGCAGCAGTAATTCTAGAGGAGCCCTGGTGGTTCTAGAAGGTTTGGACCGCTGTGGTAAGACTACACAGTCCAGTAGATTAGTCCAATACTTAGAAGGACTTGGCCATTCTGCTGAACTTTTGCGGTTTCCTGATAGAACTACAAGTGTTGGACAAATGATATCTTCCTATCTTTCAAACGAATCACAGCTGGATGACCACACAATACATCTTCTCTTCAGTGCCAATCGATGGGAGAAGAGATCGTTGATGGAAACAAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCAGCTGCCAAAGGACTTGGTATCGAATGGTGCAAGGCACCGGAGGTTGGCTTGCTAGCTCCGGACTTGGTACTGTACCTAGACATATCTCCAGAGAAAGCTGTAGAAAGAGGAGGTTATGGATGCGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTACCAAGTTTTGCATGATTCCTCTTGGAAGATAATTGATGCTTGCCAACCCATGGAAGATATTGAGAAGCAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGACGCCACAACGGGGAACCGCTTTCCGAGCTTTGGTTAAGTTGA

Protein sequence

MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS

Homology

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BLAST of MC08g0959 vs. ExPASy Swiss-Prot
Match: Q0WW55 (Thymidylate kinase OS=Arabidopsis thaliana OX=3702 GN=ZEU1 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 9.3e-88
Identity = 168/253 (66.40%), Postives = 200/253 (79.05%), Query Frame = 0


Query: 5   FHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSS-NSRGALVVLEGLDRC 64
           F  SF A+ + RS +  L+    + +R     +N +S +R  SS   RGAL+VLEGLDR 
Sbjct: 13  FSRSFRALASPRSLNYPLQCIKRSSVR--MESSNFSSGVRTESSVKPRGALIVLEGLDRS 72

Query: 65  GKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRW 124
           GK+TQ ++L+ +L GLGH  EL RFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRW
Sbjct: 73  GKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRW 132

Query: 125 EKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPE 184
           EKRSLME KLK+GTTLIVDRYSYSGVAFS+AKGL IEWCKAPE+GLLAPD VLYLDISPE
Sbjct: 133 EKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPE 192

Query: 185 KAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKR 244
           +A ERGGYG ERYE++EFQ KVA +YQ L DSSWKII+A   ME++EK++Q++VL  VK 
Sbjct: 193 RAAERGGYGDERYERVEFQKKVADFYQTLGDSSWKIINASDAMEEVEKKIQQVVLDQVKE 252

Query: 245 RHNGEPLSELWLS 257
              G+PLS LW S
Sbjct: 253 CTEGKPLSLLWSS 263

[Close]

BLAST of MC08g0959 vs. ExPASy Swiss-Prot
Match: P23919 (Thymidylate kinase OS=Homo sapiens OX=9606 GN=DTYMK PE=1 SV=4)

HSP 1 Score: 208.4 bits (529), Expect = 9.8e-53
Identity = 100/208 (48.08%), Postives = 149/208 (71.63%), Query Frame = 0


Query: 51  RGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLD 110
           RGAL+VLEG+DR GK+TQS +LV+ L   GH AELLRFP+R+T +G+++SSYL  +S ++
Sbjct: 5   RGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVE 64

Query: 111 DHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAK-GLGIEWCKAPEVGL 170
           DH++HLLFSANRWE+  L++ KL  G TL+VDRY++SGVAF+ AK    ++WCK P+VGL
Sbjct: 65  DHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGL 124

Query: 171 LAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQ-YYQVLHDS--SWKIIDACQPM 230
             PDLVL+L +    A +RG +G ERYE   FQ +  + ++Q++ D+  +WK++DA + +
Sbjct: 125 PKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSI 184

Query: 231 EDIEKQLQEIVLGCVKRRHNGEPLSELW 255
           E + + ++ +    + R    +PL ELW
Sbjct: 185 EAVHEDIRVLSEDAI-RTATEKPLGELW 211

[Close]

BLAST of MC08g0959 vs. ExPASy Swiss-Prot
Match: P97930 (Thymidylate kinase OS=Mus musculus OX=10090 GN=Dtymk PE=1 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 8.3e-52
Identity = 99/211 (46.92%), Postives = 146/211 (69.19%), Query Frame = 0


Query: 48  SNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNES 107
           ++ RGAL+VLEG+DR GKTTQ  +LV  L   GH AELLRFP+R+T +G++++SYL  ++
Sbjct: 2   ASRRGALIVLEGVDRAGKTTQGLKLVTALCASGHRAELLRFPERSTEIGKLLNSYLEKKT 61

Query: 108 QLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAK-GLGIEWCKAPE 167
           +L+DH++HLLFSANRWE+  L++ KL  G TL++DRY++SGVAF+ AK    ++WCK P+
Sbjct: 62  ELEDHSVHLLFSANRWEQVPLIKAKLNQGVTLVLDRYAFSGVAFTGAKENFSLDWCKQPD 121

Query: 168 VGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDS---SWKIIDAC 227
           VGL  PDL+L+L +    A  RG +G ERYE   FQ +V   +Q L +    +WK++DA 
Sbjct: 122 VGLPKPDLILFLQLQLLDAAARGEFGLERYETGTFQKQVLLCFQQLMEEKNLNWKVVDAS 181

Query: 228 QPMEDIEKQLQEIVLGCVKRRHNGEPLSELW 255
           + +E++ K+++      + R     PL ELW
Sbjct: 182 KSIEEVHKEIRAHSEDAI-RNAAQRPLGELW 211

[Close]

BLAST of MC08g0959 vs. ExPASy Swiss-Prot
Match: Q54GN2 (Thymidylate kinase OS=Dictyostelium discoideum OX=44689 GN=dtymk PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 5.5e-48
Identity = 93/188 (49.47%), Postives = 128/188 (68.09%), Query Frame = 0


Query: 51  RGALVVLEGLDRCGKTTQSSRLVQYLEGLGH-SAELLRFPDRTTSVGQMISSYLSNESQL 110
           RG  ++ EG+DR GK+TQ   L  ++  +     + LRFPDRTT +GQ+I+ YL N + +
Sbjct: 18  RGLFILFEGVDRVGKSTQVQSLTNHISNVQKLPTKSLRFPDRTTPIGQIINQYLQNATNM 77

Query: 111 DDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGL 170
           DD  +HLLFS+NRWE R  +   L +GT ++VDRYSYSGVA+SAAKG+  +WC A E GL
Sbjct: 78  DDRALHLLFSSNRWEARDSILELLNNGTNIVVDRYSYSGVAYSAAKGIDFDWCYACEKGL 137

Query: 171 LAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYY-QVLHDSSWKIIDACQPMED 230
             PDL+ YL +S E A +RG YG ERYE+LEFQ K+ Q Y + L D  WKII+A + +++
Sbjct: 138 PKPDLIFYLSMSSEDATKRGEYGGERYEKLEFQKKIKQIYEEKLVDDQWKIINANRSIDE 197

Query: 231 IEKQLQEI 237
           I  ++  I
Sbjct: 198 ISNEISSI 205

[Close]

BLAST of MC08g0959 vs. ExPASy Swiss-Prot
Match: P36590 (Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tmp1 PE=2 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 8.0e-47
Identity = 94/197 (47.72%), Postives = 127/197 (64.47%), Query Frame = 0


Query: 47  SSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNE 106
           S  +RG L+V+EGLDR GK+TQ   LV  L      AEL +FPDRTT++G+ I  YL   
Sbjct: 2   SKQNRGRLIVIEGLDRSGKSTQCQLLVDKLISQHEKAELFKFPDRTTAIGKKIDDYLKES 61

Query: 107 SQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPE 166
            QL+D  IHLLFSANRWE    +  ++  G T I+DRY++SG+AFSAAKGL  EWCK+P+
Sbjct: 62  VQLNDQVIHLLFSANRWETIQYIYEQINKGVTCILDRYAFSGIAFSAAKGLDWEWCKSPD 121

Query: 167 VGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDS------SWKII 226
            GL  PDLV++L++ P  A  RG YG ERYE++E Q KV + +Q L          +  +
Sbjct: 122 RGLPRPDLVIFLNVDPRIAATRGQYGEERYEKIEMQEKVLKNFQRLQKEFREEGLEFITL 181

Query: 227 DACQPMEDIEKQLQEIV 238
           DA   +ED+  Q+ ++V
Sbjct: 182 DASSSLEDVHSQIVDLV 198

[Close]

BLAST of MC08g0959 vs. NCBI nr
Match: XP_022153422.1 (thymidylate kinase-like isoform X1 [Momordica charantia])

HSP 1 Score: 515 bits (1327), Expect = 2.48e-184
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 60
           MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL
Sbjct: 1   MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 60

Query: 61  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 120
           DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA
Sbjct: 61  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 120

Query: 121 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 180
           NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI
Sbjct: 121 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 180

Query: 181 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 240
           SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC
Sbjct: 181 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 240

Query: 241 VKRRHNGEPLSELWLS 256
           VKRRHNGEPLSELWLS
Sbjct: 241 VKRRHNGEPLSELWLS 256

[Close]

BLAST of MC08g0959 vs. NCBI nr
Match: XP_022998308.1 (thymidylate kinase isoform X1 [Cucurbita maxima])

HSP 1 Score: 456 bits (1173), Expect = 8.25e-161
Identity = 224/250 (89.60%), Postives = 237/250 (94.80%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV AR+SSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 10  LSFEAVIARKSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 69

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 70  TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 129

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           SLMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 130 SLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 189

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 190 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 249

Query: 247 GEPLSELWLS 256
           GEPLS+LWLS
Sbjct: 250 GEPLSQLWLS 259

[Close]

BLAST of MC08g0959 vs. NCBI nr
Match: KAG7036529.1 (Thymidylate kinase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 456 bits (1172), Expect = 9.77e-161
Identity = 224/249 (89.96%), Postives = 236/249 (94.78%), Query Frame = 0


Query: 8   SFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTT 67
           SF AV ARRSSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKTT
Sbjct: 6   SFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKTT 65

Query: 68  QSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKRS 127
           QSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKRS
Sbjct: 66  QSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKRS 125

Query: 128 LMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVE 187
           LMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA E
Sbjct: 126 LMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKASE 185

Query: 188 RGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNG 247
           RGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HNG
Sbjct: 186 RGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHNG 245

Query: 248 EPLSELWLS 256
           EPLS+LWLS
Sbjct: 246 EPLSQLWLS 254

[Close]

BLAST of MC08g0959 vs. NCBI nr
Match: XP_022948490.1 (thymidylate kinase isoform X1 [Cucurbita moschata])

HSP 1 Score: 455 bits (1170), Expect = 2.36e-160
Identity = 224/250 (89.60%), Postives = 236/250 (94.40%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV ARRSSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 10  LSFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 69

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 70  TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 129

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           S METKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 130 SSMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 189

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 190 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 249

Query: 247 GEPLSELWLS 256
           GEPLS+LWLS
Sbjct: 250 GEPLSQLWLS 259

[Close]

BLAST of MC08g0959 vs. NCBI nr
Match: XP_023523991.1 (thymidylate kinase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 454 bits (1169), Expect = 3.36e-160
Identity = 224/250 (89.60%), Postives = 236/250 (94.40%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV ARRSSS +LKI S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 10  LSFEAVIARRSSSFILKISSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 69

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 70  TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 129

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           SLMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 130 SLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 189

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKV QYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 190 ERGGYGCERYEQLEFQSKVDQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 249

Query: 247 GEPLSELWLS 256
           GEP+S+LWLS
Sbjct: 250 GEPISQLWLS 259

[Close]

BLAST of MC08g0959 vs. ExPASy TrEMBL
Match: A0A6J1DIU8 (dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1)

HSP 1 Score: 515 bits (1327), Expect = 1.20e-184
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 60
           MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL
Sbjct: 1   MLFRFHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 60

Query: 61  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 120
           DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA
Sbjct: 61  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 120

Query: 121 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 180
           NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI
Sbjct: 121 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 180

Query: 181 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 240
           SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC
Sbjct: 181 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 240

Query: 241 VKRRHNGEPLSELWLS 256
           VKRRHNGEPLSELWLS
Sbjct: 241 VKRRHNGEPLSELWLS 256

[Close]

BLAST of MC08g0959 vs. ExPASy TrEMBL
Match: A0A6J1KGD6 (dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1)

HSP 1 Score: 456 bits (1173), Expect = 3.99e-161
Identity = 224/250 (89.60%), Postives = 237/250 (94.80%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV AR+SSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 10  LSFEAVIARKSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 69

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 70  TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 129

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           SLMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 130 SLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 189

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 190 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 249

Query: 247 GEPLSELWLS 256
           GEPLS+LWLS
Sbjct: 250 GEPLSQLWLS 259

[Close]

BLAST of MC08g0959 vs. ExPASy TrEMBL
Match: A0A6J1G9Z9 (dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1)

HSP 1 Score: 455 bits (1170), Expect = 1.14e-160
Identity = 224/250 (89.60%), Postives = 236/250 (94.40%), Query Frame = 0


Query: 7   LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDRCGKT 66
           LSF AV ARRSSS +LK+ S   IR IQMGTN NS+L+GC+SNSRGALVVLEGLDRCGKT
Sbjct: 10  LSFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVLEGLDRCGKT 69

Query: 67  TQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRWEKR 126
           TQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN+SQLDDHTIHLLFSANRWEKR
Sbjct: 70  TQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLLFSANRWEKR 129

Query: 127 SLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAV 186
           S METKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYL+ISPEKA 
Sbjct: 130 SSMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLNISPEKAS 189

Query: 187 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKRRHN 246
           ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK+IDACQPMEDIEKQLQE+VL CVK+ HN
Sbjct: 190 ERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKVIDACQPMEDIEKQLQEMVLECVKKCHN 249

Query: 247 GEPLSELWLS 256
           GEPLS+LWLS
Sbjct: 250 GEPLSQLWLS 259

[Close]

BLAST of MC08g0959 vs. ExPASy TrEMBL
Match: A0A6J1DHF1 (dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1)

HSP 1 Score: 453 bits (1165), Expect = 1.73e-160
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 35  MGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTS 94
           MGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTS
Sbjct: 1   MGTNHNSVLRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTS 60

Query: 95  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAA 154
           VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAA
Sbjct: 61  VGQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAA 120

Query: 155 KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHD 214
           KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHD
Sbjct: 121 KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHD 180

Query: 215 SSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS 256
           SSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS
Sbjct: 181 SSWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS 222

[Close]

BLAST of MC08g0959 vs. ExPASy TrEMBL
Match: A0A6J1DDX5 (dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111019882 PE=3 SV=1)

HSP 1 Score: 450 bits (1158), Expect = 7.71e-159
Identity = 231/256 (90.23%), Postives = 235/256 (91.80%), Query Frame = 0


Query: 3   FRFH--LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGL 62
           F FH  LSF AVTARRSSS+ LKIPS N I+KIQMGTN NSVLRGCSSNSRGALVVLEGL
Sbjct: 4   FTFHKPLSFRAVTARRSSSSPLKIPSKNSIQKIQMGTNQNSVLRGCSSNSRGALVVLEGL 63

Query: 63  DRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSA 122
           DRCGKTTQSSRLVQYLEGLG SAEL RFPDRTTSVGQMISSYLSN+SQLDD TIHLLFSA
Sbjct: 64  DRCGKTTQSSRLVQYLEGLGFSAELWRFPDRTTSVGQMISSYLSNKSQLDDRTIHLLFSA 123

Query: 123 NRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDI 182
           NRWEKRSLME+KLKSGTTLIVDRYSYSGVAFSAAKGL IEWCKAPEVGLLA D VLYLDI
Sbjct: 124 NRWEKRSLMESKLKSGTTLIVDRYSYSGVAFSAAKGLDIEWCKAPEVGLLASDSVLYLDI 183

Query: 183 SPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGC 242
           SPE A ERGGYG ERYEQLEFQ KV QYYQVLHDSSWKIIDACQ MEDIEKQLQEIVLGC
Sbjct: 184 SPENAAERGGYGRERYEQLEFQRKVYQYYQVLHDSSWKIIDACQTMEDIEKQLQEIVLGC 243

Query: 243 VKRRHNGEPLSELWLS 256
           VKR HNGEPLSELWLS
Sbjct: 244 VKRCHNGEPLSELWLS 259

[Close]

BLAST of MC08g0959 vs. TAIR 10
Match: AT5G59440.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 324.7 bits (831), Expect = 6.6e-89
Identity = 168/253 (66.40%), Postives = 200/253 (79.05%), Query Frame = 0


Query: 5   FHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSS-NSRGALVVLEGLDRC 64
           F  SF A+ + RS +  L+    + +R     +N +S +R  SS   RGAL+VLEGLDR 
Sbjct: 13  FSRSFRALASPRSLNYPLQCIKRSSVR--MESSNFSSGVRTESSVKPRGALIVLEGLDRS 72

Query: 65  GKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRW 124
           GK+TQ ++L+ +L GLGH  EL RFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRW
Sbjct: 73  GKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRW 132

Query: 125 EKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPE 184
           EKRSLME KLK+GTTLIVDRYSYSGVAFS+AKGL IEWCKAPE+GLLAPD VLYLDISPE
Sbjct: 133 EKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPE 192

Query: 185 KAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKR 244
           +A ERGGYG ERYE++EFQ KVA +YQ L DSSWKII+A   ME++EK++Q++VL  VK 
Sbjct: 193 RAAERGGYGDERYERVEFQKKVADFYQTLGDSSWKIINASDAMEEVEKKIQQVVLDQVKE 252

Query: 245 RHNGEPLSELWLS 257
              G+PLS LW S
Sbjct: 253 CTEGKPLSLLWSS 263

[Close]

BLAST of MC08g0959 vs. TAIR 10
Match: AT5G59440.3 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 324.7 bits (831), Expect = 6.6e-89
Identity = 168/253 (66.40%), Postives = 200/253 (79.05%), Query Frame = 0


Query: 5   FHLSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSS-NSRGALVVLEGLDRC 64
           F  SF A+ + RS +  L+    + +R     +N +S +R  SS   RGAL+VLEGLDR 
Sbjct: 21  FSRSFRALASPRSLNYPLQCIKRSSVR--MESSNFSSGVRTESSVKPRGALIVLEGLDRS 80

Query: 65  GKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANRW 124
           GK+TQ ++L+ +L GLGH  EL RFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRW
Sbjct: 81  GKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRW 140

Query: 125 EKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISPE 184
           EKRSLME KLK+GTTLIVDRYSYSGVAFS+AKGL IEWCKAPE+GLLAPD VLYLDISPE
Sbjct: 141 EKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPE 200

Query: 185 KAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWKIIDACQPMEDIEKQLQEIVLGCVKR 244
           +A ERGGYG ERYE++EFQ KVA +YQ L DSSWKII+A   ME++EK++Q++VL  VK 
Sbjct: 201 RAAERGGYGDERYERVEFQKKVADFYQTLGDSSWKIINASDAMEEVEKKIQQVVLDQVKE 260

Query: 245 RHNGEPLSELWLS 257
              G+PLS LW S
Sbjct: 261 CTEGKPLSLLWSS 271

[Close]

BLAST of MC08g0959 vs. TAIR 10
Match: AT5G59440.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 7.3e-88
Identity = 160/221 (72.40%), Postives = 186/221 (84.16%), Query Frame = 0


Query: 37  TNHNSVLRGCSS-NSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSV 96
           +N +S +R  SS   RGAL+VLEGLDR GK+TQ ++L+ +L GLGH  EL RFPDR TSV
Sbjct: 4   SNFSSGVRTESSVKPRGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDRETSV 63

Query: 97  GQMISSYLSNESQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAK 156
           GQMIS+YLSN+SQLDDHTIHLLFSANRWEKRSLME KLK+GTTLIVDRYSYSGVAFS+AK
Sbjct: 64  GQMISAYLSNKSQLDDHTIHLLFSANRWEKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAK 123

Query: 157 GLGIEWCKAPEVGLLAPDLVLYLDISPEKAVERGGYGCERYEQLEFQSKVAQYYQVLHDS 216
           GL IEWCKAPE+GLLAPD VLYLDISPE+A ERGGYG ERYE++EFQ KVA +YQ L DS
Sbjct: 124 GLDIEWCKAPEIGLLAPDSVLYLDISPERAAERGGYGDERYERVEFQKKVADFYQTLGDS 183

Query: 217 SWKIIDACQPMEDIEKQLQEIVLGCVKRRHNGEPLSELWLS 257
           SWKII+A   ME++EK++Q++VL  VK    G+PLS LW S
Sbjct: 184 SWKIINASDAMEEVEKKIQQVVLDQVKECTEGKPLSLLWSS 224

The following BLAST results are available for this feature:

BLAST of MC08g0959 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WW55	9.3e-88	66.40	Thymidylate kinase OS=Arabidopsis thaliana OX=3702 GN=ZEU1 PE=2 SV=1	[more]
P23919	9.8e-53	48.08	Thymidylate kinase OS=Homo sapiens OX=9606 GN=DTYMK PE=1 SV=4	[more]
P97930	8.3e-52	46.92	Thymidylate kinase OS=Mus musculus OX=10090 GN=Dtymk PE=1 SV=2	[more]
Q54GN2	5.5e-48	49.47	Thymidylate kinase OS=Dictyostelium discoideum OX=44689 GN=dtymk PE=3 SV=1	[more]
P36590	8.0e-47	47.72	Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284...	[more]

BLAST of MC08g0959 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153422.1	2.48e-184	100.00	thymidylate kinase-like isoform X1 [Momordica charantia]	[more]
XP_022998308.1	8.25e-161	89.60	thymidylate kinase isoform X1 [Cucurbita maxima]	[more]
KAG7036529.1	9.77e-161	89.96	Thymidylate kinase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022948490.1	2.36e-160	89.60	thymidylate kinase isoform X1 [Cucurbita moschata]	[more]
XP_023523991.1	3.36e-160	89.60	thymidylate kinase isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC08g0959 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIU8	1.20e-184	100.00	dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1	[more]
A0A6J1KGD6	3.99e-161	89.60	dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1	[more]
A0A6J1G9Z9	1.14e-160	89.60	dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1	[more]
A0A6J1DHF1	1.73e-160	100.00	dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1	[more]
A0A6J1DDX5	7.71e-159	90.23	dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111019882 PE=3 SV=1	[more]

BLAST of MC08g0959 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59440.1	6.6e-89	66.40	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G59440.3	6.6e-89	66.40	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G59440.2	7.3e-88	72.40	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 46..255 e-value: 6.1E-69 score: 233.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 51..247
IPR018094	Thymidylate kinase	TIGRFAM	TIGR00041	TIGR00041	coord: 51..232 e-value: 5.8E-38 score: 128.5
IPR018094	Thymidylate kinase	HAMAP	MF_00165	Thymidylate_kinase	coord: 53..241 score: 16.357859
IPR039430	Thymidylate kinase-like domain	PFAM	PF02223	Thymidylate_kin	coord: 57..232 e-value: 1.1E-45 score: 155.6
None	No IPR available	PANTHER	PTHR10344:SF1	THYMIDYLATE KINASE	coord: 49..251
None	No IPR available	PANTHER	PTHR10344	THYMIDYLATE KINASE	coord: 49..251
None	No IPR available	CDD	cd01672	TMPK	coord: 54..238 e-value: 2.33569E-31 score: 113.132
IPR018095	Thymidylate kinase, conserved site	PROSITE	PS01331	THYMIDYLATE_KINASE	coord: 140..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0959.1	MC08g0959.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006233	dTDP biosynthetic process
biological_process	GO:0046940	nucleoside monophosphate phosphorylation
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004798	thymidylate kinase activity