MC08g0923 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC08g0923
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	4-coumarate CoA ligase
Location	MC08: 7367318 .. 7371337 (-)
RNA-Seq Expression	MC08g0923
Synteny	MC08g0923

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATGGCCACCGAAACTCTTCCGAACGACTTCATTTTCCGGTCAAAACTCCCCGACATCTACATCCCCAAACACCTTCCCCTGCACTCCTACTGCCTCCACGAGAACGCCGCCAAGATCGGCCACCGGACCTGCCTCATCAATGGCGTCACCGGCGACTCCTACACCTACGCCGACGTCGATCTCACCGCCCGCAAGGTCGCCTCCGGTCTGAACAAGCTCGGCATTGCGCAAGGCGATGTCCTAATGCTCCTCCTCCCGAATTCGCCTGAATTCGTCTTCGCCTTCCTCGGCGCGTCCTACCGCGGCGCGATCATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCGAAGCAAGCCAAAGGATCGAAGGCGAAATTGATCATTACGCAAGCGTCGTATTACGACAAAGTAAAGGAAATAACAGAGGAATTACCAGATGTCAAAATCATGACCGTCGATTCTCCGCCGGACGGTTGCTTGTCGTTCTCCGATTTGACTCAGGCCGACGAGCGGGAGATTCCGGCGGTGGATATCGGTCCAGACGACGTCGTGGCGCTGCCGTATTCCTCCGGCACGACGGGATTGCCGAAAGGCGTGATGCTCACTCACAAAGGCTTAGTCACCAGTGTGGCTCAGCAAGTGGACGGCGATAATCCGAATCTGTACTTCCGAAACGACGACGTAATCTTGTGCGTTTTGCCGCTCTTCCATATTTACTCGCTGAATTCCGTTCTCCTCTGCGGATTGCGCGCCGGCGCCACCATTCTGATTTTGCCTAAATTTGAGATCGCTTCTCTGCTTCAATTGGTACAGAAATACAGAGTGTCCATCGCGCCGGTGGTTCCGCCGATCGTTCTCGCCATCGCGAAGTCGCCGGACCTCGATAAATACGACCTTTCTTCCATCAGAGTTCTGAAATCCGGCGGCGCTCCGCTCGGGAAGGAGCTTGAAGATACCGTGAGAGCTAAGTTTCCGAAGGCAGTGCTCGGCCAGGTACCAGAAATTCCTAATACTAATATCTGATGATTAATATTTAATTGTTTAAGATTAATTACTTAATTATAATTAGGATTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTCCTTTTTGATTCGTGATTAGTCAAATTTATTGATGTGGAGAAGACTACACTTACGACCAAATGGTCCCCAAGTCAGGGTCGAAGACAAATTTTTGCTCATAAAACAAATTAAAAATTTTGGTTAATTCAATATTTATTTATTTACATGAAAATGAAAATCTATTTTGGCTTACTCTTGCTTTGCAGGTTTCGTCCACCGATAAATCAAATGGTAACAAAAATTGTCAGTCTAAAGACTACCTAAAAAATTTCAATCTAACTTGAAAAAGTATTTCCAAATATCAAAATCGTTACTCTTTTCATTTAATATTACACCAAAAAGAACAAAAAAAGATAGAGATATATGAACTACTTCAACTATATTTTGGCTTCAAATTCTTACAAGCCACGTCACTTTTTTTTAGTTAGGAAACAGTCACTTTATATTTTGTACTGTATCATATGAATTTAGTATTTCAAGCCATGTATATATGAATTTGAAATTATTGATACAATATTTATTTAAAATAATACAAACATTTTAACATTAAAGATCAAAACAAGAATTTAACTAGAAATTTGAAGACCAAAACAAAATATTCAAAATTATAATAACATCAAATAAATCGAGTTTAATAGTTTAAAAATCGTAATAAATTTTAAGGCTAAATATATCAAATTATTCATAAATTTTAGGTAATTCTCAAAATTCCCACTAATGCATTTTCTCAATTTAGTCCACTTAGATATTTCACATAAAAGGAAATGGATTTTATAAATGGACAGATGGAATTTTTTGGTAGACAGCTAGATTTCATGTGTCGAAGTAATTAACACGTAAATTACATGTGTATAGAAGGAGCAATCTCATTGTTAACGACAAAAGCTCTGTAGTATTATCATGAGGTTTTTTTTTTTAATAAATAATTTACATAACATTTTAAAGACGTGGCATAATTTGAGTAGTTAATTACTCAGATTTCTGTAAAGTGAGAGGAAACGTTTTTGTTCTGGATTGGCAGGGATACGGAATGACAGAGGCCGGCCCGGTGCTAACCATGGGTTTGGCGTTTGCAAAAGAGCCGTTTCCGATGAAGCCAGGTGCCTGCGGCACCGTCGTCCGGAACGCCGAGATGAAGATCGTCGACACCGAGAACGGCGCCTCACTTCCCCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGCTATCTGAACAATCCGGAGGCGACGGTGGCCACCATAGACGCCGACGGATGGTTGCACACCGGCGACATCGGCTTCATCGACGACGACGACGAGCTTTTCATCGTCGATCGGTTGAAGGAATTGATCAAGTACAAGGGATTCCAAGTCGCGCCGGCGGAACTCGAGGCTCTGTTGCTCACTCATACGGTCATCACCGATGCCGCCGTTGTCCCGTGAGAACCGTTAACTCTCGTTTGATTCTTTTTAAAATCGACGGTGGTGATCGGTTTATTTATTTATTTTAATTATTTTATTATTATTATTTTTTTGTGTTGAATTTGCAGAATGAAAGATGAACAAGCTGGAGAGGTTCCGGTTGCGTTTGTGGTGAAATCGAAGAGTTCTGAAGTCACTGAAGATGAAATTAAACAATTTATATCAAAACAGGTACTTTTTTTTATTTTTTTCTTATTATTTTACTCAAAACTTTTTAAAAACTTATTTCAATGCCTGAAGTTTCTAGTCTGTTTTTATTTTAATCTCTCAAATTTTAAAAATAATTATTTTAATTATAGTCATTATTTTATCGTGAATGATTTAATGAATAAATGTAAATTGCTAATTCAGTCGGTGAAATTTCAAAGTTTAAGATCCTATTAAGTATTAAATAGAAAATTAAAATTATACAAAAAAAAAAGAAAATTAAAAGTTTAAATACATGTTCAACACTTAATTTATTTAGAAAAAAATTTGAAAATTTGAGACCGTTTTTAAAGCTTAAAAGATACAACTATGAAAGTTTAAAGTAAATTTTAATATAGCTTAACTGGTTAAAGTAAGTATATATATATATATATTAAAAATAGGAAAATTGTAGAGGGTAACAAGAATATTTTATGTATTTTAAAATATGTCACCTGTTTTTTTTATTTTTATATATAGCACAAATTTTCACTTATAGTAATAGTATATCAGATACTCATATTAATATTATATTAGACACTCATATCAATAGTATATCATATATATATTGCACAGACACTTGTATCAATAGTGTATCACATACATATTCCACAAACACTCATATCAGTGGTATATCAGATGTATATCACATATATATTACAAATATATCAGACACCCATATCAGTAGTATATATCAGATGTATATCACATACATAGTAAGATATATTACAGATGTATATCATTTGGATAAAATTATCATTTTAAAATTAGACCTCCGTTTTTTTTTTAAGTTCTGCATCTGGAAAAACTAAATGTGGTTGCTATTTTGCTAATTTTATTAAATGTTTTTGTCATATTTACAACTGTCCTAACAAATATTATGAAAGGAAAAAGTTACGAAAGTGTAAGACTAAATTTGTAGCTCAACCGTAAATAAATAACGTAGTTTTAAGATAATAATTGAAGGGTAAAAAGTAAACTTATTTTGAAGCGGTAGGTATTGAAATAAAATAAGTTTAAATAGTCCGCAATTTAAATAAAATAAATTAATTAGGTATAAATAAAATATAATTTTGGGAATTAAAGTGACGATTTTGTGAAACTAAAACGAGCAGGTGGTGTTCTACAAGAGAATCAACCGGGTGTTTTTCATTGACGCAATTCCCAAATCGCCCTCCGGCAAGATCTTGAGGAAGGAGTTGAGGGCAAAATTGGCAGATGTTTCTCCTAATTCT

mRNA sequence

AAAATGGCCACCGAAACTCTTCCGAACGACTTCATTTTCCGGTCAAAACTCCCCGACATCTACATCCCCAAACACCTTCCCCTGCACTCCTACTGCCTCCACGAGAACGCCGCCAAGATCGGCCACCGGACCTGCCTCATCAATGGCGTCACCGGCGACTCCTACACCTACGCCGACGTCGATCTCACCGCCCGCAAGGTCGCCTCCGGTCTGAACAAGCTCGGCATTGCGCAAGGCGATGTCCTAATGCTCCTCCTCCCGAATTCGCCTGAATTCGTCTTCGCCTTCCTCGGCGCGTCCTACCGCGGCGCGATCATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCGAAGCAAGCCAAAGGATCGAAGGCGAAATTGATCATTACGCAAGCGTCGTATTACGACAAAGTAAAGGAAATAACAGAGGAATTACCAGATGTCAAAATCATGACCGTCGATTCTCCGCCGGACGGTTGCTTGTCGTTCTCCGATTTGACTCAGGCCGACGAGCGGGAGATTCCGGCGGTGGATATCGGTCCAGACGACGTCGTGGCGCTGCCGTATTCCTCCGGCACGACGGGATTGCCGAAAGGCGTGATGCTCACTCACAAAGGCTTAGTCACCAGTGTGGCTCAGCAAGTGGACGGCGATAATCCGAATCTGTACTTCCGAAACGACGACGTAATCTTGTGCGTTTTGCCGCTCTTCCATATTTACTCGCTGAATTCCGTTCTCCTCTGCGGATTGCGCGCCGGCGCCACCATTCTGATTTTGCCTAAATTTGAGATCGCTTCTCTGCTTCAATTGGTACAGAAATACAGAGTGTCCATCGCGCCGGTGGTTCCGCCGATCGTTCTCGCCATCGCGAAGTCGCCGGACCTCGATAAATACGACCTTTCTTCCATCAGAGTTCTGAAATCCGGCGGCGCTCCGCTCGGGAAGGAGCTTGAAGATACCGTGAGAGCTAAGTTTCCGAAGGCAGTGCTCGGCCAGGGATACGGAATGACAGAGGCCGGCCCGGTGCTAACCATGGGTTTGGCGTTTGCAAAAGAGCCGTTTCCGATGAAGCCAGGTGCCTGCGGCACCGTCGTCCGGAACGCCGAGATGAAGATCGTCGACACCGAGAACGGCGCCTCACTTCCCCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGCTATCTGAACAATCCGGAGGCGACGGTGGCCACCATAGACGCCGACGGATGGTTGCACACCGGCGACATCGGCTTCATCGACGACGACGACGAGCTTTTCATCGTCGATCGGTTGAAGGAATTGATCAAGTACAAGGGATTCCAAGTCGCGCCGGCGGAACTCGAGGCTCTGTTGCTCACTCATACGGTCATCACCGATGCCGCCGTTGTCCCAATGAAAGATGAACAAGCTGGAGAGGTTCCGGTTGCGTTTGTGGTGAAATCGAAGAGTTCTGAAGTCACTGAAGATGAAATTAAACAATTTATATCAAAACAGGTGGTGTTCTACAAGAGAATCAACCGGGTGTTTTTCATTGACGCAATTCCCAAATCGCCCTCCGGCAAGATCTTGAGGAAGGAGTTGAGGGCAAAATTGGCAGATGTTTCTCCTAATTCT

Coding sequence (CDS)

AAAATGGCCACCGAAACTCTTCCGAACGACTTCATTTTCCGGTCAAAACTCCCCGACATCTACATCCCCAAACACCTTCCCCTGCACTCCTACTGCCTCCACGAGAACGCCGCCAAGATCGGCCACCGGACCTGCCTCATCAATGGCGTCACCGGCGACTCCTACACCTACGCCGACGTCGATCTCACCGCCCGCAAGGTCGCCTCCGGTCTGAACAAGCTCGGCATTGCGCAAGGCGATGTCCTAATGCTCCTCCTCCCGAATTCGCCTGAATTCGTCTTCGCCTTCCTCGGCGCGTCCTACCGCGGCGCGATCATGACGGCGGCGAATCCTTTCTTCACGGCGGCGGAGATCGCGAAGCAAGCCAAAGGATCGAAGGCGAAATTGATCATTACGCAAGCGTCGTATTACGACAAAGTAAAGGAAATAACAGAGGAATTACCAGATGTCAAAATCATGACCGTCGATTCTCCGCCGGACGGTTGCTTGTCGTTCTCCGATTTGACTCAGGCCGACGAGCGGGAGATTCCGGCGGTGGATATCGGTCCAGACGACGTCGTGGCGCTGCCGTATTCCTCCGGCACGACGGGATTGCCGAAAGGCGTGATGCTCACTCACAAAGGCTTAGTCACCAGTGTGGCTCAGCAAGTGGACGGCGATAATCCGAATCTGTACTTCCGAAACGACGACGTAATCTTGTGCGTTTTGCCGCTCTTCCATATTTACTCGCTGAATTCCGTTCTCCTCTGCGGATTGCGCGCCGGCGCCACCATTCTGATTTTGCCTAAATTTGAGATCGCTTCTCTGCTTCAATTGGTACAGAAATACAGAGTGTCCATCGCGCCGGTGGTTCCGCCGATCGTTCTCGCCATCGCGAAGTCGCCGGACCTCGATAAATACGACCTTTCTTCCATCAGAGTTCTGAAATCCGGCGGCGCTCCGCTCGGGAAGGAGCTTGAAGATACCGTGAGAGCTAAGTTTCCGAAGGCAGTGCTCGGCCAGGGATACGGAATGACAGAGGCCGGCCCGGTGCTAACCATGGGTTTGGCGTTTGCAAAAGAGCCGTTTCCGATGAAGCCAGGTGCCTGCGGCACCGTCGTCCGGAACGCCGAGATGAAGATCGTCGACACCGAGAACGGCGCCTCACTTCCCCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGCTATCTGAACAATCCGGAGGCGACGGTGGCCACCATAGACGCCGACGGATGGTTGCACACCGGCGACATCGGCTTCATCGACGACGACGACGAGCTTTTCATCGTCGATCGGTTGAAGGAATTGATCAAGTACAAGGGATTCCAAGTCGCGCCGGCGGAACTCGAGGCTCTGTTGCTCACTCATACGGTCATCACCGATGCCGCCGTTGTCCCAATGAAAGATGAACAAGCTGGAGAGGTTCCGGTTGCGTTTGTGGTGAAATCGAAGAGTTCTGAAGTCACTGAAGATGAAATTAAACAATTTATATCAAAACAGGTGGTGTTCTACAAGAGAATCAACCGGGTGTTTTTCATTGACGCAATTCCCAAATCGCCCTCCGGCAAGATCTTGAGGAAGGAGTTGAGGGCAAAATTGGCAGATGTTTCTCCTAATTCT

Protein sequence

KMATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDIGPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHIYSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEVPVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLADVSPNS

Homology

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BLAST of MC08g0923 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 5.4e-234
Identity = 407/543 (74.95%), Postives = 457/543 (84.16%), Query Frame = 0


Query: 4   TETLPN-DFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDL 63
           TET  + D IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING     YTYA+V+L
Sbjct: 7   TETKQSGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVEL 66

Query: 64  TARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQA 123
           T RKVA GLNKLGI Q D +M+LLPNSPEFVFAF+GASY GAI T ANP FT AE+ KQA
Sbjct: 67  TCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQA 126

Query: 124 KGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDIG 183
           K S AK+IITQ+ +  KVK+   E  DVK++ +DS P+GCL FS+LTQ+DE EIP V I 
Sbjct: 127 KASSAKIIITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQ 186

Query: 184 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHIY 243
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+N NLY  ++DV++CVLPLFHIY
Sbjct: 187 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 246

Query: 244 SLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDL 303
           SLNS+LLCGLR GA ILI+ KF+IA  L+L+QKY+VSI P VPPIVLAIAKSP +D YDL
Sbjct: 247 SLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDL 306

Query: 304 SSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 363
           SS+R + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GA
Sbjct: 307 SSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 366

Query: 364 CGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTG 423
           CGTVVRNAEMKIVD + G SLPRN PGEICIRGDQIMKGYLN+PEAT  TID +GWLHTG
Sbjct: 367 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTG 426

Query: 424 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEVP 483
           DIGFID+DDELFIVDRLKELIKYKGFQVAPAE+EALLL H  I+DAAVVPMKDEQAGEVP
Sbjct: 427 DIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVP 486

Query: 484 VAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLADV 543
           VAFVV+S  S +TEDE+K FISKQV+FYKR+ RVFF++ +PKSPSGKILRK+LRA+LA  
Sbjct: 487 VAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAG 546

Query: 544 SPN 546
            PN
Sbjct: 547 VPN 547

[Close]

BLAST of MC08g0923 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.0e-232
Identity = 409/544 (75.18%), Postives = 458/544 (84.19%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           M  +T   D IFRSKLPDIYIP HLPLHSYC  EN ++   R CLING     YTYADV+
Sbjct: 1   MEKDTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVE 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L +RKVA+GL+K GI   D +M+LLPNSPEFVFAF+GASY GAI T ANP FT AE+ KQ
Sbjct: 61  LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AK S AK+I+TQA + +KVK+   E  DVKI+ +DS P+GCL FS LTQA+E +IP V+I
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY  ++DV+LCVLPLFHI
Sbjct: 181 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLR GA ILI+ KF+I S L+L+Q+Y+V+I P VPPIVLAIAKSP +D YD
Sbjct: 241 YSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSS+R + SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K G
Sbjct: 301 LSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVD + G SLPRN  GEICIRGDQIMKGYLN+PEAT  TID +GWL+T
Sbjct: 361 ACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIG+IDDDDELFIVDRLKELIKYKGFQVAPAELEALLL H  I+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVV+S  S +TEDE+K FISKQV+FYKRI RVFF+DAIPKSPSGKILRK+LRAKLA 
Sbjct: 481 PVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAA 540

Query: 542 VSPN 546
             PN
Sbjct: 541 GLPN 542

[Close]

BLAST of MC08g0923 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 1.3e-232
Identity = 407/532 (76.50%), Postives = 458/532 (86.09%), Query Frame = 0


Query: 10  DFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVAS 69
           +FIFRSKLPDIYIP HLPLHSYC  EN ++   R CLING TG+  TYADVDLT+RKVA+
Sbjct: 12  EFIFRSKLPDIYIPNHLPLHSYCF-ENISQFKDRPCLINGATGEIITYADVDLTSRKVAA 71

Query: 70  GLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 129
           GL+KLGI QGDV+MLLL NSPEFV+AFL ASY GAI+T ANPF+T AE+AKQA  SK KL
Sbjct: 72  GLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASKTKL 131

Query: 130 IITQASYYDKVKEITEELPDVKIMTVDSPP--DGCLSFSDLTQADEREIPAVDIGPDDVV 189
           +IT A Y DKVKE T     VK+M VD+PP    CL FS+LTQADE EIPAV I PDDVV
Sbjct: 132 VITLAGYIDKVKEFTGGESGVKVMCVDAPPPESECLHFSELTQADETEIPAVKIHPDDVV 191

Query: 190 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHIYSLNSV 249
           ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYF  +DVILCVLPLFHIYSLNS+
Sbjct: 192 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHIYSLNSI 251

Query: 250 LLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSSIRV 309
           LLCGLR GA ILI+ KFEI+ LL+L++K++V+IAP VPPIVL++AK PDL +YDLSSIR 
Sbjct: 252 LLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYDLSSIRT 311

Query: 310 LKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 369
           + SGGAP+GKELED V+ K P A LGQGYGMTEAGPVL+M LAFAKEPFP+K GACGTVV
Sbjct: 312 VMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSGACGTVV 371

Query: 370 RNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDIGFI 429
           RNAEMKIVD + GASLPRN  GEICIRG QIMKGY+N+ EAT  TID  GWLHTGDIGFI
Sbjct: 372 RNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHTGDIGFI 431

Query: 430 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEVPVAFVV 489
           D+DDELFIVDRLKELIKYKGFQVAPAELE++L++H  ITDAAVVPMKDE AGEVPVAFVV
Sbjct: 432 DNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEVPVAFVV 491

Query: 490 KSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 540
           +S  S++TE++IKQ+ISKQVVFYKRIN+ FFI+ IPK+PSGKILRK LRAKL
Sbjct: 492 RSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAKL 542

[Close]

BLAST of MC08g0923 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 2.9e-232
Identity = 402/531 (75.71%), Postives = 457/531 (86.06%), Query Frame = 0


Query: 10  DFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVAS 69
           D IFRSKLPDIYIPKHLPLH+YC  EN +K+G ++CLING TG+++TY+ V+L +RKVAS
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 69

Query: 70  GLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 129
           GLNKLGI QGD +MLLLPNSPE+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S+AKL
Sbjct: 70  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 129

Query: 130 IITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDIGPDDVVAL 189
           IITQA Y DKVK+   E  +++I+ +D  P  CL FS L +ADE E+P V I  DDVVAL
Sbjct: 130 IITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVVAL 189

Query: 190 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHIYSLNSVLL 249
           PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLY  ++DV++C+LPLFHIYSLN+VL 
Sbjct: 190 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLC 249

Query: 250 CGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSSIRVLK 309
           CGLRAG TILI+ KF+I   L+L+QKY+V+I P VPPIVLAIAKSP +DKYDLSS+R + 
Sbjct: 250 CGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVM 309

Query: 310 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 369
           SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVVRN
Sbjct: 310 SGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRN 369

Query: 370 AEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDIGFIDD 429
           AEMKIVD E  ASLPRN  GEICIRGDQIMKGYLN+PE+T  TID +GWLHTGDIGFIDD
Sbjct: 370 AEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDD 429

Query: 430 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEVPVAFVVKS 489
           DDELFIVDRLKE+IKYKGFQVAPAELEALLLTH  I+DAAVVPM DE+AGEVPVAFVV++
Sbjct: 430 DDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRT 489

Query: 490 KSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 541
                TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRA++A
Sbjct: 490 NGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIA 538

[Close]

BLAST of MC08g0923 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 3.9e-232
Identity = 403/531 (75.89%), Postives = 456/531 (85.88%), Query Frame = 0


Query: 10  DFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVAS 69
           D IFRSKLPDIYIPKHLPLH+YC  EN +K+G ++CLING TG+++TY+ V+L +RKVAS
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 69

Query: 70  GLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAKL 129
           GLNKLGI QGD +MLLLPNSPE+ FAFLGASYRGAI T ANPFFT+AE+ KQ K S AKL
Sbjct: 70  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKL 129

Query: 130 IITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDIGPDDVVAL 189
           IITQA Y DKVK+   E  +++I+ +D  P  CL FS L +ADE E+P V I  DDVVAL
Sbjct: 130 IITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVVAL 189

Query: 190 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHIYSLNSVLL 249
           PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLY  ++DV++C+LPLFHIYSLN+VL 
Sbjct: 190 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLC 249

Query: 250 CGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSSIRVLK 309
           CGLRAG TILI+ KF+I   L+L+QKY+V+I P VPPIVLAIAKSP +DKYDLSS+R + 
Sbjct: 250 CGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVM 309

Query: 310 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 369
           SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVVRN
Sbjct: 310 SGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRN 369

Query: 370 AEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDIGFIDD 429
           AEMKIVD E  ASLPRN  GEICIRGDQIMKGYLN+PE+T  TID +GWLHTGDIGFIDD
Sbjct: 370 AEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDD 429

Query: 430 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEVPVAFVVKS 489
           DDELFIVDRLKE+IKYKGFQVAPAELEALLLTH  I+DAAVVPM DE+AGEVPVAFVV++
Sbjct: 430 DDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRT 489

Query: 490 KSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 541
                TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRAK+A
Sbjct: 490 NGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIA 538

[Close]

BLAST of MC08g0923 vs. NCBI nr
Match: KAG6581611.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 983 bits (2540), Expect = 0.0
Identity = 494/545 (90.64%), Postives = 517/545 (94.86%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VTG+S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L ARKVASGLNKLGIAQGDV+MLLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIRV+K GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTH  I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVV+ K+SEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 542 VSPNS 546
             PNS
Sbjct: 541 GFPNS 545

[Close]

BLAST of MC08g0923 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 981 bits (2537), Expect = 0.0
Identity = 493/545 (90.46%), Postives = 517/545 (94.86%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VTG+S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L ARKVASGLNKLGIAQGDV+ML+L NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIRV+K GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTH  I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVV+ K+SEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 542 VSPNS 546
             PNS
Sbjct: 541 GFPNS 545

[Close]

BLAST of MC08g0923 vs. NCBI nr
Match: XP_023526871.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 491/545 (90.09%), Postives = 515/545 (94.50%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           MA E L NDFIFRSKLP IYIP HLPLHSYCLHEN AKIGHRTCLIN VTG+S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPHIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L ARKVASGLNKLGIAQGDV+MLLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGS AKLI+TQ+SYY+KVKEITEE+PDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEEVPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIRV+K GGAPLGKELEDTVR KFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRTKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTH  I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVV+ K+SEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 542 VSPNS 546
             PNS
Sbjct: 541 GFPNS 545

[Close]

BLAST of MC08g0923 vs. NCBI nr
Match: XP_022983944.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 975 bits (2521), Expect = 0.0
Identity = 491/545 (90.09%), Postives = 515/545 (94.50%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VT +S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L ARKVASGLNKLGIAQGDV++LLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIRV+K GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTH  I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVV+ K+SEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 542 VSPNS 546
             P S
Sbjct: 541 GFPIS 545

[Close]

BLAST of MC08g0923 vs. NCBI nr
Match: XP_038904387.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 487/545 (89.36%), Postives = 515/545 (94.50%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           M  ET+ ND IFRSKLPDIYIPKHLPLHSYCLHENAAKIG RTCLINGVTG+S+TY DVD
Sbjct: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L+ARKVA+GLNKLGI + DV+MLLLPNSPEFVF FLGASY GAIMTAANPFFTA EIAKQ
Sbjct: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGSKAKLI+T +SYY+KVKEITEELPDVKIMTVDSPPDGCL F DL +ADER+IP V+I
Sbjct: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY+RN+DVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAG TILI+PKFEI  LLQLV+KYRVSIAP+VPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIRV+K GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVDTE G+SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIGFIDD+DELFIVDRLKELIKYKGFQVAPAELEALLLTH VI+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVVK K+SEVTEDEIKQF+SKQVVFYKRINRVFFIDAIPKSPSGKILRK+LRAKLA 
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540

Query: 542 VSPNS 546
             PNS
Sbjct: 541 AFPNS 545

[Close]

BLAST of MC08g0923 vs. ExPASy TrEMBL
Match: A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)

HSP 1 Score: 981 bits (2537), Expect = 0.0
Identity = 493/545 (90.46%), Postives = 517/545 (94.86%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VTG+S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L ARKVASGLNKLGIAQGDV+ML+L NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIRV+K GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTH  I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVV+ K+SEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 542 VSPNS 546
             PNS
Sbjct: 541 GFPNS 545

[Close]

BLAST of MC08g0923 vs. ExPASy TrEMBL
Match: A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)

HSP 1 Score: 975 bits (2521), Expect = 0.0
Identity = 491/545 (90.09%), Postives = 515/545 (94.50%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           MA E L NDFIFRSKLPDIYIP HLPLHSYCLHEN AKIGHRTCLIN VT +S+TY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L ARKVASGLNKLGIAQGDV++LLL NSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGS AKLI+TQ+SYY+KVKEITEELPDVKIMTVDSPPDGCLSF+DL QADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDG+NPNLY+RN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAG TILI+PKFEI SLLQLVQK++VSIAP+VPPIVLAIAKSPDLDKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIRV+K GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTH  I+DAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVV+ K+SEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 542 VSPNS 546
             P S
Sbjct: 541 GFPIS 545

[Close]

BLAST of MC08g0923 vs. ExPASy TrEMBL
Match: A0A5A7SWF8 (4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00110 PE=4 SV=1)

HSP 1 Score: 961 bits (2483), Expect = 0.0
Identity = 485/545 (88.99%), Postives = 515/545 (94.50%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           M  ET+ ND IFRSKLPDIYIP HLPLHSYCL ENAAKIGHRTCLINGVTG+S+T+ DVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           LTARKVASGLNKLGI + DV+MLLLPNSPEFVFAFLGAS+ GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGSKAKLIITQ+SYY+K+KEITEELP+VKIMTVDSP DGCL F DL +ADEREIP V+I
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY+ N+DVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAG+TILI+PKFEI  LLQLV+KY V++AP+VPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIRV+KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAEMKIVDTE G+SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID DGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIGFID+DDELFIVDRLKELIKYKGFQVAPAELEALLLTH VI+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 541
           PVAFVVK K+SE TEDEIKQFISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 542 VSPNS 546
             PNS
Sbjct: 541 AFPNS 545

[Close]

BLAST of MC08g0923 vs. ExPASy TrEMBL
Match: A0A6J1G9T7 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)

HSP 1 Score: 960 bits (2482), Expect = 0.0
Identity = 487/539 (90.35%), Postives = 515/539 (95.55%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           MATET+ +DFIFRSKLPDIYIPKHLPLHSYCL E  A+IGHRTCLIN VTG+S+TY+DVD
Sbjct: 1   MATETV-HDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L ARK ASGL KLGIA+GDV+MLLLPNSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGSKAKLIITQ+SYY+KVKEITE+L D KIMTVDSP  GCLSF+DL Q  + +IPAV+I
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY+RNDDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAGATILI+PKFEI SLLQLV+KYRVSIAP+VPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIR++KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAE+KIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEAT ATID++GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTH VI+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVVK K+SEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 538

[Close]

BLAST of MC08g0923 vs. ExPASy TrEMBL
Match: A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)

HSP 1 Score: 955 bits (2469), Expect = 0.0
Identity = 484/539 (89.80%), Postives = 512/539 (94.99%), Query Frame = 0


Query: 2   MATETLPNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVD 61
           MA ET+ +D IFRSKLPDIYIPKHLPLHSYCL E  A+IGHRTCLIN VTG+S+TY+DVD
Sbjct: 1   MANETV-HDIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 62  LTARKVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 121
           L ARK ASGL KLGIA+GDV+MLLLPNSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 122 AKGSKAKLIITQASYYDKVKEITEELPDVKIMTVDSPPDGCLSFSDLTQADEREIPAVDI 181
           AKGSKAKL ITQ+SYY+KVKEITE+LPD KIMTVDSPP GCLSF+DL Q  + EIPAV+I
Sbjct: 121 AKGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180

Query: 182 GPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHI 241
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY+RNDDVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 242 YSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYD 301
           YSLNSVLLCGLRAGATILI+PKFEI SLLQLV+KYRVSIAP+VPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 302 LSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 361
           LSSIR++KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 362 ACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHT 421
           ACGTVVRNAE+KIVDTENG SLPRNTPGEICI+GDQIMKGYLNNPEAT ATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHT 420

Query: 422 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEV 481
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTH VI+DAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 482 PVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVVK K+S+VTEDEIKQFISKQVVFYKRINRVFFI AIPKSPSGKILRKELRAKLA
Sbjct: 481 PVAFVVKLKNSKVTEDEIKQFISKQVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLA 538

[Close]

BLAST of MC08g0923 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 770.8 bits (1989), Expect = 7.5e-223
Identity = 384/537 (71.51%), Postives = 454/537 (84.54%), Query Frame = 0


Query: 9   NDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVA 68
           ND IFRS+LPDIYIP HLPLH Y + EN ++   + CLING TG+ YTYADV +T+RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 69  SGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAK 128
           +GL+ LG+ Q DV+M+LLPNSPE V  FL AS+ GAI T+ANPFFT AEI+KQAK S AK
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 129 LIITQASYYDKVKEITEELPDVKIMTVDSP--PDGCLSFSDLTQADEREIPAV--DIGPD 188
           LI+TQ+ Y DK+K +  +   V I+T DS   P+ CL FS+LTQ++E  + ++   I P+
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 198

Query: 189 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHIYSL 248
           DVVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLYF  DDVILCVLP+FHIY+L
Sbjct: 199 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 258

Query: 249 NSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSS 308
           NS++LC LR GATILI+PKFEI  LL+ +Q+ +V++A VVPPIVLAIAKSP+ +KYDLSS
Sbjct: 259 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 318

Query: 309 IRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 368
           +R++KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACG
Sbjct: 319 VRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 378

Query: 369 TVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDI 428
           TVVRNAEMKI+D + G SLPRN PGEICIRG+QIMKGYLN+P AT +TID DGWLHTGD+
Sbjct: 379 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 438

Query: 429 GFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEVPVA 488
           GFIDDDDELFIVDRLKELIKYKGFQVAPAELE+LL+ H  I D AVV MK+E AGEVPVA
Sbjct: 439 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 498

Query: 489 FVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAD 542
           FVV+SK S ++EDEIKQF+SKQVVFYKRIN+VFF D+IPK+PSGKILRK+LRA+LA+
Sbjct: 499 FVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLAN 552

[Close]

BLAST of MC08g0923 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 765.4 bits (1975), Expect = 3.1e-221
Identity = 388/541 (71.72%), Postives = 449/541 (82.99%), Query Frame = 0


Query: 9   NDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVA 68
           +D IFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  YTY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 69  SGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAK 128
           +  +KLG+ Q DV+MLLLPN PEFV +FL AS+RGA  TAANPFFT AEIAKQAK S  K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 129 LIITQASYYDKVKEITEELPDVKIMTVDSP-----PDGCLSFSDLTQAD---EREIPAVD 188
           LIIT+A Y DK+K +  +   V I+ +D       P+GCL F++LTQ+       I +V+
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 189 IGPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFH 248
           I PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLYF +DDVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 249 IYSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKY 308
           IY+LNS++LCGLR GA ILI+PKFEI  LL+L+Q+ +V++AP+VPPIVLAIAKS + +KY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 309 DLSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 368
           DLSSIRV+KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 369 GACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLH 428
           GACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP AT  TID DGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 429 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGE 488
           TGDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ H  ITD AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 489 VPVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 542
           VPVAFVVKSK SE++ED++KQF+SKQVVFYKRIN+VFF ++IPK+PSGKILRK+LRAKLA
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 559

[Close]

BLAST of MC08g0923 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 709.9 bits (1831), Expect = 1.6e-204
Identity = 361/507 (71.20%), Postives = 416/507 (82.05%), Query Frame = 0


Query: 9   NDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKVA 68
           +D IFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  YTY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 69  SGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKAK 128
           +  +KLG+ Q DV+MLLLPN PEFV +FL AS+RGA  TAANPFFT AEIAKQAK S  K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 129 LIITQASYYDKVKEITEELPDVKIMTVDSP-----PDGCLSFSDLTQAD---EREIPAVD 188
           LIIT+A Y DK+K +  +   V I+ +D       P+GCL F++LTQ+       I +V+
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 189 IGPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFH 248
           I PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLYF +DDVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 249 IYSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKY 308
           IY+LNS++LCGLR GA ILI+PKFEI  LL+L+Q+ +V++AP+VPPIVLAIAKS + +KY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 309 DLSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 368
           DLSSIRV+KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 369 GACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLH 428
           GACGTVVRNAEMKIVD + G SL RN PGEICIRG QIMKGYLNNP AT  TID DGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 429 TGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGE 488
           TGDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ H  ITD AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 489 VPVAFVVKSKSSEVTEDEIKQFISKQV 508
           VPVAFVVKSK SE++ED++KQF+SKQV
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQV 525

[Close]

BLAST of MC08g0923 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 694.9 bits (1792), Expect = 5.2e-200
Identity = 346/535 (64.67%), Postives = 429/535 (80.19%), Query Frame = 0


Query: 8   PNDFIFRSKLPDIYIPKHLPLHSYCLHENAAKIGHRTCLINGVTGDSYTYADVDLTARKV 67
           P   IFRSKLPDI IP HLPLH+YC  E  + +  + CLI G TG SYTY +  L  R+V
Sbjct: 30  PTPRIFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRV 89

Query: 68  ASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGSKA 127
           ASGL KLGI +GDV+M+LL NS EFVF+F+GAS  GA+ T ANPF+T+ E+ KQ K S A
Sbjct: 90  ASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGA 149

Query: 128 KLIITQASYYDKVKEITEELPDVKIMTVDSP-PDGCLSFSDLTQADERE--IPAVDIGPD 187
           KLIIT + Y DK+K + E L    ++T D P P+ CL FS L   DE       VDIG D
Sbjct: 150 KLIITHSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGD 209

Query: 188 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVLPLFHIYSL 247
           D  ALP+SSGTTGLPKGV+LTHK L+TSVAQQVDGDNPNLY +++DVILCVLPLFHIYSL
Sbjct: 210 DAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSL 269

Query: 248 NSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPDLDKYDLSS 307
           NSVLL  LR+GAT+L++ KFEI +LL L+Q++RV+IA +VPP+V+A+AK+P ++ YDLSS
Sbjct: 270 NSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSS 329

Query: 308 IRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 367
           +R + SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CG
Sbjct: 330 VRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCG 389

Query: 368 TVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDADGWLHTGDI 427
           TVVRNAE+K+V  E   SL  N PGEICIRG QIMK YLN+PEAT ATID +GWLHTGDI
Sbjct: 390 TVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDI 449

Query: 428 GFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDEQAGEVPVA 487
           G++D+DDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I DAAVVP  DE AGEVPVA
Sbjct: 450 GYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVA 509

Query: 488 FVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 540
           FVV+S  +++TE+++K++++KQVVFYKR+++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 510 FVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

[Close]

BLAST of MC08g0923 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 674.9 bits (1740), Expect = 5.6e-194
Identity = 335/543 (61.69%), Postives = 420/543 (77.35%), Query Frame = 0


Query: 9   NDFIFRSKLPDIYIPKHLPLHSYCLHE---NAAKIGHRTCLINGVTGDSYTYADVDLTAR 68
           +DFIFRSKLPDI+IP HLPL  Y       +       TC+I+G TG   TYADV    R
Sbjct: 25  HDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNMR 84

Query: 69  KVASGLNKLGIAQGDVLMLLLPNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQAKGS 128
           ++A+G+++LGI  GDV+MLLLPNSPEF  +FL  +Y GA+ T ANPF+T  EIAKQAK S
Sbjct: 85  RIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKAS 144

Query: 129 KAKLIITQASYYDKVKEITEELPDVKIMTVD---------SPPDGCLSFSDLTQADEREI 188
            AK+IIT+    DK+  +  +   V I+ +D         S  DGC+SF++LTQADE E+
Sbjct: 145 AAKMIITKKCLVDKLTNLKND--GVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETEL 204

Query: 189 PAVDIGPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVILCVL 248
               I P+D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDG+NPNL F  +DVILC L
Sbjct: 205 LKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFL 264

Query: 249 PLFHIYSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIAKSPD 308
           P+FHIY+L++++L  +R GA +LI+P+FE+  +++L+Q+Y+V++ PV PP+VLA  KSP+
Sbjct: 265 PMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSPE 324

Query: 309 LDKYDLSSIRVLKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPF 368
            ++YDLSS+R++ SG A L KELED VR KFP A+ GQGYGMTE+G V    LAFAK PF
Sbjct: 325 TERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNPF 384

Query: 369 PMKPGACGTVVRNAEMKIVDTENGASLPRNTPGEICIRGDQIMKGYLNNPEATVATIDAD 428
             K GACGTV+RNAEMK+VDTE G SLPRN  GEIC+RG Q+MKGYLN+PEAT  TID D
Sbjct: 385 KTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDKD 444

Query: 429 GWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHTVITDAAVVPMKDE 488
           GWLHTGDIGF+DDDDE+FIVDRLKELIK+KG+QVAPAELEALL++H  I DAAVV MKDE
Sbjct: 445 GWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDE 504

Query: 489 QAGEVPVAFVVKSKSSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELR 540
            A EVPVAFV +S+ S++TED++K +++KQVV YKRI  VFFI+ IPK+ SGKILRK+LR
Sbjct: 505 VADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDLR 564

The following BLAST results are available for this feature:

BLAST of MC08g0923 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24145	5.4e-234	74.95	4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1	[more]
O24146	1.0e-232	75.18	4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1	[more]
M4ISH0	1.3e-232	76.50	4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1	[more]
P14912	2.9e-232	75.71	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1	[more]
P14913	3.9e-232	75.89	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1	[more]

BLAST of MC08g0923 vs. NCBI nr


Match Name	Identity	Description
KAG6581611.1	90.64	4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022935389.1	90.46	4-coumarate--CoA ligase 1-like [Cucurbita moschata]	[more]
XP_023526871.1	90.09	4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022983944.1	90.09	4-coumarate--CoA ligase 1-like [Cucurbita maxima]	[more]
XP_038904387.1	89.36	4-coumarate--CoA ligase 1-like [Benincasa hispida]	[more]

BLAST of MC08g0923 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1F599	90.46	4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=...	[more]
A0A6J1J912	90.09	4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 ...	[more]
A0A5A7SWF8	88.99	4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1G9T7	90.35	4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KCG9	89.80	4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 ...	[more]

BLAST of MC08g0923 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21240.1	7.5e-223	71.51	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	3.1e-221	71.72	4-coumarate:CoA ligase 1	[more]
AT1G51680.3	1.6e-204	71.20	4-coumarate:CoA ligase 1	[more]
AT1G65060.1	5.2e-200	64.67	4-coumarate:CoA ligase 3	[more]
AT3G21230.1	5.6e-194	61.69	4-coumarate:CoA ligase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 34..445 e-value: 4.7E-110 score: 368.1
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 7..439 e-value: 1.2E-172 score: 576.4
None	No IPR available	GENE3D	3.30.300.30		coord: 440..542 e-value: 5.1E-32 score: 112.6
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 7..541
None	No IPR available	PANTHER	PTHR24096:SF382	4-COUMARATE--COA LIGASE 2-LIKE	coord: 7..541
None	No IPR available	CDD	cd05904	4CL	coord: 22..535 e-value: 0.0 score: 765.627
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 14..542
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 454..529 e-value: 4.1E-15 score: 56.5
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 189..200

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC08g0923.1	MC08g0923.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016874	ligase activity