MC08g0520 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g0520
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein of Unknown Function (DUF239)
LocationMC08: 4196355 .. 4198861 (+)
RNA-Seq ExpressionMC08g0520
SyntenyMC08g0520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCACACATATTTTCATTGCTATAAAGGATTTCCAAACCACTTCTTCAATGATGTAATGTTTGAATGATTTCTAAGCAATTCAACAAGCACATTTGTTAGCCATTGGTTTGTGATTTAAGGCATCGAAAAGACCCGAGTTTCGGTGAGCGTTTTCGATGGGGAAGAGATTCTCTTGCAGTGGTTTGAGGTTCTTCAAAATGTTGGTACTGACATTGACAGTCATAGTTTGTGGAGTTGTGGAGGGTGGTTCACTTTCTAAACAGAAGAGTTTGGAAGTTGTGAAGAAAATTAACTCTCTTAGAAAGCAAGCAATAAAAAGCATTCAGGTACCAATCTGAGTTTCTTCTTCTTCTCTTCTCATTTGATCTTTGAAACTTATTTTCTTTTTCCATAACCAATGTACAAACACCTGAAAGCTTACCTTATTCCCCCCTAATCCTTTAGCTTTATTCTCTAAAAGTCTAAGAATTCGAAAAGCCTACCGTATCCTTACGAGGCGAGAAATTTGAAGGACAAATCAAGAATTCAGGCAATCAGTTCAAGGCCTTGTTTCAGACAATCAATTTGATTGGAATGAGCTAAAACTTGCAGCATCGTTGTATATGTTTAGCTTTTCCATCATCTCTATGGCAATGTTAAACTTTTATAGCAGAAGATTACATGTTTTCAATGGACACTATGCCTTTTTAGCTGCACTTTTCTTTCTGTTGCTCGTAGAGCGAAGACGGCGACATCATAGATTGCGTTAGAGTTTACGAGCAGCCTGCTTTTGATCATCCTGCTCTAAGAAATCATACCATTCAGGTCTGCTTTTTCTTTCATTCTGAAGTTCTGTTTCACGTTGAAGAAACTGTTTTTTGTACTTCTTTAAACCATTAACCAATGGTTTGGCATAGATGGCACCTACTTATGATCCCACCATGGAAGAGCATTCAAAGAAAGCAACAGAAAAAAGGGAAGGGATGGAGGAAAAGGACTCCATGGCTGTGAAACAAACATGGAGGAAAAGCGGCAGTTGTCCCAAAGGAACAATACCGCTTCGAAGGATCCGAAAAGATGTCCTGCTCAAAGCTGACTCGGTAGAACGCTATGGAAGAAAGAGACCGACGTATTCGCATAAAATCGCTCAGCTTTCCAACAACCAAACCTCGCATGCTCTGCTAGTGAATCGTTCGGTTTGACTATTGAACATTTCTTTTCCCTTTTGAATTCCATTCTTTTCTTCCTCTTACCATCTACTTGATCATGGTGCAAAACAGAAGGCATTTCTGCACACTGAAGGATACAACTACAATGCAGCCAAAGGAGACATTAAAGTCTGTAACCCCAAGGTCGAGAGCGATGACGAGTATAGTACTTCCCAAGTGGCTCTACTAAATGGTGCTTACTATCATTTCGAGTGCGTCGAATCCGGATGGGCAGTAAGCATAACTTGATACCAAAGAGCTCAAGCTTTATCATAACTGCACTTTATACCCAGCAACATAGTTGCCTCTCATTTGAATGTCTACTGATTTTAACTTCTTTACATCATGATTTCTTCAACCCTTGAGGTAAATCCAAGTGTTTACGGAGATCGACAGACTCGACTGTTCGTGTATTGGACGGTAAGTCTAATATGCAGGCAACAAGCTATCTCCCATTGCTTGGATGCCATATGATAGAAGAAAGTACTCTCTCAAAAACAGAACTAACTGAAAATTGTTTTCTTTTGGTGGTATTAGGCTGATGCTTCCCATAAAACAGGCTGCTTTGATCTCACTTGCCCTGGTTTTGTTCAAACCAGCAACGAAATTGCTCTTGGTTCTGCTATATATCCCATCTCAACTCCAAATGGGCTTCCATATGAAATAACTCTGTTCCTTTTCAGAGTAAGAAATGATGCAGTTTCCTCTCTAAAATAGTAAATCCAATTCATTGCTTCCTTTTTGTCATGGATTTTTCATCTTCCCTATAGGATTTACAGACAAATAATTGGTGGGTGCAATATGGGGAAAGCATTAACATAGGTTATTGGCCCGCTGAGTTATTCCATGCTCTAAGTCATACTGCAGAGACTGTCCAGTGGGGAGGGGAAGTTTACAGCACAAAGTTAGGAGGGCCTCCCCACACTACAACAGGTATGGGCAATGGAAGGTTTCCTGACTTTGTTTTCGGCAATTCGGGTTGGGTAAAACGGATGCGAATTCGGGACAACTCGATGGTGTTGAAGTTTCCTGGATGGGTTGAGCATTACTCTGATGAATATGATTGTTATGACGTCGATTTTGTCCGAGATTATTTAGAAGATCCTGAACTATACTATGGGGGGCCTGGTAAAAATCCAAAGTGTCCCTAACAGACTTCTACAAAAATGGGATGATCATCTCTTGCTTTGATTAGGTTACTTCATGTAATGAACAATAATACCATCAAGAAGAAAGTTCTCTCTTTTGTTTCAGATTCTTACTTGCAACAATGCTGTCTTTGTTCCCAGAAAGAACTTTTCAGTCAATTTTTACCTGGC

mRNA sequence

AGCACACATATTTTCATTGCTATAAAGGATTTCCAAACCACTTCTTCAATGATGTAATGTTTGAATGATTTCTAAGCAATTCAACAAGCACATTTGTTAGCCATTGGTTTGTGATTTAAGGCATCGAAAAGACCCGAGTTTCGGTGAGCGTTTTCGATGGGGAAGAGATTCTCTTGCAGTGGTTTGAGGTTCTTCAAAATGTTGGTACTGACATTGACAGTCATAGTTTGTGGAGTTGTGGAGGGTGGTTCACTTTCTAAACAGAAGAGTTTGGAAGTTGTGAAGAAAATTAACTCTCTTAGAAAGCAAGCAATAAAAAGCATTCAGAGCGAAGACGGCGACATCATAGATTGCGTTAGAGTTTACGAGCAGCCTGCTTTTGATCATCCTGCTCTAAGAAATCATACCATTCAGATGGCACCTACTTATGATCCCACCATGGAAGAGCATTCAAAGAAAGCAACAGAAAAAAGGGAAGGGATGGAGGAAAAGGACTCCATGGCTGTGAAACAAACATGGAGGAAAAGCGGCAGTTGTCCCAAAGGAACAATACCGCTTCGAAGGATCCGAAAAGATGTCCTGCTCAAAGCTGACTCGGTAGAACGCTATGGAAGAAAGAGACCGACGTATTCGCATAAAATCGCTCAGCTTTCCAACAACCAAACCTCGCATGCTCTGCTAGTGAATCGTTCGAAGGCATTTCTGCACACTGAAGGATACAACTACAATGCAGCCAAAGGAGACATTAAAGTCTGTAACCCCAAGGTCGAGAGCGATGACGAGTATAGTACTTCCCAAGTGGCTCTACTAAATGGTGCTTACTATCATTTCGAGTGCGTCGAATCCGGATGGGCAGTAAATCCAAGTGTTTACGGAGATCGACAGACTCGACTGTTCGTGTATTGGACGGCTGATGCTTCCCATAAAACAGGCTGCTTTGATCTCACTTGCCCTGGTTTTGTTCAAACCAGCAACGAAATTGCTCTTGGTTCTGCTATATATCCCATCTCAACTCCAAATGGGCTTCCATATGAAATAACTCTGTTCCTTTTCAGAGATTTACAGACAAATAATTGGTGGGTGCAATATGGGGAAAGCATTAACATAGGTTATTGGCCCGCTGAGTTATTCCATGCTCTAAGTCATACTGCAGAGACTGTCCAGTGGGGAGGGGAAGTTTACAGCACAAAGTTAGGAGGGCCTCCCCACACTACAACAGGTATGGGCAATGGAAGGTTTCCTGACTTTGTTTTCGGCAATTCGGGTTGGGTAAAACGGATGCGAATTCGGGACAACTCGATGGTGTTGAAGTTTCCTGGATGGGTTGAGCATTACTCTGATGAATATGATTGTTATGACGTCGATTTTGTCCGAGATTATTTAGAAGATCCTGAACTATACTATGGGGGGCCTGGTAAAAATCCAAAGTGTCCCTAACAGACTTCTACAAAAATGGGATGATCATCTCTTGCTTTGATTAGGTTACTTCATGTAATGAACAATAATACCATCAAGAAGAAAGTTCTCTCTTTTGTTTCAGATTCTTACTTGCAACAATGCTGTCTTTGTTCCCAGAAAGAACTTTTCAGTCAATTTTTACCTGGC

Coding sequence (CDS)

ATGGGGAAGAGATTCTCTTGCAGTGGTTTGAGGTTCTTCAAAATGTTGGTACTGACATTGACAGTCATAGTTTGTGGAGTTGTGGAGGGTGGTTCACTTTCTAAACAGAAGAGTTTGGAAGTTGTGAAGAAAATTAACTCTCTTAGAAAGCAAGCAATAAAAAGCATTCAGAGCGAAGACGGCGACATCATAGATTGCGTTAGAGTTTACGAGCAGCCTGCTTTTGATCATCCTGCTCTAAGAAATCATACCATTCAGATGGCACCTACTTATGATCCCACCATGGAAGAGCATTCAAAGAAAGCAACAGAAAAAAGGGAAGGGATGGAGGAAAAGGACTCCATGGCTGTGAAACAAACATGGAGGAAAAGCGGCAGTTGTCCCAAAGGAACAATACCGCTTCGAAGGATCCGAAAAGATGTCCTGCTCAAAGCTGACTCGGTAGAACGCTATGGAAGAAAGAGACCGACGTATTCGCATAAAATCGCTCAGCTTTCCAACAACCAAACCTCGCATGCTCTGCTAGTGAATCGTTCGAAGGCATTTCTGCACACTGAAGGATACAACTACAATGCAGCCAAAGGAGACATTAAAGTCTGTAACCCCAAGGTCGAGAGCGATGACGAGTATAGTACTTCCCAAGTGGCTCTACTAAATGGTGCTTACTATCATTTCGAGTGCGTCGAATCCGGATGGGCAGTAAATCCAAGTGTTTACGGAGATCGACAGACTCGACTGTTCGTGTATTGGACGGCTGATGCTTCCCATAAAACAGGCTGCTTTGATCTCACTTGCCCTGGTTTTGTTCAAACCAGCAACGAAATTGCTCTTGGTTCTGCTATATATCCCATCTCAACTCCAAATGGGCTTCCATATGAAATAACTCTGTTCCTTTTCAGAGATTTACAGACAAATAATTGGTGGGTGCAATATGGGGAAAGCATTAACATAGGTTATTGGCCCGCTGAGTTATTCCATGCTCTAAGTCATACTGCAGAGACTGTCCAGTGGGGAGGGGAAGTTTACAGCACAAAGTTAGGAGGGCCTCCCCACACTACAACAGGTATGGGCAATGGAAGGTTTCCTGACTTTGTTTTCGGCAATTCGGGTTGGGTAAAACGGATGCGAATTCGGGACAACTCGATGGTGTTGAAGTTTCCTGGATGGGTTGAGCATTACTCTGATGAATATGATTGTTATGACGTCGATTTTGTCCGAGATTATTTAGAAGATCCTGAACTATACTATGGGGGGCCTGGTAAAAATCCAAAGTGTCCCTAA

Protein sequence

MGKRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSEDGDIIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSKAFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP
Homology
BLAST of MC08g0520 vs. NCBI nr
Match: XP_038886336.1 (uncharacterized protein LOC120076548 [Benincasa hispida])

HSP 1 Score: 743 bits (1917), Expect = 1.75e-268
Identity = 351/424 (82.78%), Postives = 387/424 (91.27%), Query Frame = 0

Query: 3   KRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSEDGD 62
           KRFSC+ L F KMLV+TLTVIVCG+VEGGSL+KQKSL V KK+NSLRKQA KSIQS+DGD
Sbjct: 7   KRFSCNSLMF-KMLVVTLTVIVCGIVEGGSLTKQKSLNVEKKMNSLRKQATKSIQSKDGD 66

Query: 63  IIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWR 122
           IIDC+ +Y+QPAFDHPALRNHTIQMAPTYDPTM+EHSKKAT +REGME KDSM VKQTWR
Sbjct: 67  IIDCINIYDQPAFDHPALRNHTIQMAPTYDPTMDEHSKKATAEREGMEGKDSMVVKQTWR 126

Query: 123 KSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSKAF 182
           KSGSCPKGTIP+RRI+K +L KADS+E YGRKRP  S +IAQLSN+++SH LL N SKA 
Sbjct: 127 KSGSCPKGTIPIRRIQKHLLRKADSMEHYGRKRPASSQEIAQLSNSRSSHYLLKNHSKAC 186

Query: 183 LHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYGDR 242
           L   G NYN AKGDIKVCNPKVE DDEYSTSQVALL G YY++E VESGWAVNP VYGDR
Sbjct: 187 LLALGNNYNGAKGDIKVCNPKVEFDDEYSTSQVALLTGPYYNYEAVESGWAVNPGVYGDR 246

Query: 243 QTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDL 302
           QTRLFVYWT DASHKTGCFDLTCPGFVQTSNEIALGSAIYPIST  GLPYEIT+FLFRDL
Sbjct: 247 QTRLFVYWTVDASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTSTGLPYEITMFLFRDL 306

Query: 303 QTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFP 362
           +TNNWWVQYGESI+IGYWP+ELF+AL +TAETVQWGGEVYSTKLGGPPHT TGMGNG+FP
Sbjct: 307 KTNNWWVQYGESIDIGYWPSELFNALKYTAETVQWGGEVYSTKLGGPPHTRTGMGNGKFP 366

Query: 363 DFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKN 422
           D++ G+SGWVKR+R+RDNSMVLKFP WVEHYSDEYDCYD+DF+RDYL+DPELYYGGPGKN
Sbjct: 367 DYISGDSGWVKRIRVRDNSMVLKFPDWVEHYSDEYDCYDIDFIRDYLDDPELYYGGPGKN 426

Query: 423 PKCP 426
           PKCP
Sbjct: 427 PKCP 429

BLAST of MC08g0520 vs. NCBI nr
Match: XP_023546232.1 (uncharacterized protein LOC111805388 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724 bits (1870), Expect = 1.55e-261
Identity = 347/426 (81.46%), Postives = 380/426 (89.20%), Query Frame = 0

Query: 1   MGKRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSED 60
           MGKRF         MLV+TLTVIVCG VEGGSLSKQKSL    ++NSLRKQAIKSI+SED
Sbjct: 1   MGKRF------LLGMLVVTLTVIVCGAVEGGSLSKQKSL----RMNSLRKQAIKSIRSED 60

Query: 61  GDIIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQT 120
           GDIIDCV +Y+QPAFDHPALRNHTIQ+APTYDPT++EHSKKAT +REG EEK+SM VKQT
Sbjct: 61  GDIIDCVSIYDQPAFDHPALRNHTIQLAPTYDPTIDEHSKKATAEREGKEEKNSMVVKQT 120

Query: 121 WRKSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSK 180
           WRKSGSCP+GTIP+RRIRK VLLKA+S+ERYGRK+P +S + AQL   ++SH LL NRSK
Sbjct: 121 WRKSGSCPEGTIPIRRIRKHVLLKAESIERYGRKKPMFSQENAQLPITRSSHTLLTNRSK 180

Query: 181 AFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYG 240
           AFL T G NYNAAKGDIKVCNP+VE DDEYSTSQVALL G YY+FE +ESGWAVNP VYG
Sbjct: 181 AFLLTAGNNYNAAKGDIKVCNPRVEFDDEYSTSQVALLTGPYYNFEAIESGWAVNPGVYG 240

Query: 241 DRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFR 300
           DRQTRLFVYWTADAS KTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEI +FLFR
Sbjct: 241 DRQTRLFVYWTADASQKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEIIMFLFR 300

Query: 301 DLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGR 360
           D +T+NWWVQYGESINIGYWP ELF ALSHTAETVQWGGEVYSTK+G PPHT T MG+GR
Sbjct: 301 DFKTDNWWVQYGESINIGYWPPELFGALSHTAETVQWGGEVYSTKIGRPPHTRTAMGSGR 360

Query: 361 FPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPG 420
           FPDF+ G SGWVKRMR+RDNSMVL FPGWVEHYSDEYDCYDVDF+RDYL+DPELYYGGPG
Sbjct: 361 FPDFISGTSGWVKRMRVRDNSMVLMFPGWVEHYSDEYDCYDVDFIRDYLDDPELYYGGPG 416

Query: 421 KNPKCP 426
           KNP+CP
Sbjct: 421 KNPRCP 416

BLAST of MC08g0520 vs. NCBI nr
Match: KAG7029277.1 (hypothetical protein SDJN02_07615, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 723 bits (1865), Expect = 8.95e-261
Identity = 346/426 (81.22%), Postives = 379/426 (88.97%), Query Frame = 0

Query: 1   MGKRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSED 60
           MGKRF         MLV+TL VIVCG VEGGSLSKQKSL    ++NSLRKQAIKSI+SED
Sbjct: 1   MGKRF------LLGMLVVTLIVIVCGAVEGGSLSKQKSL----RMNSLRKQAIKSIRSED 60

Query: 61  GDIIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQT 120
           GDIIDCV +Y+QPAFDHPALRNHTIQ+APTYDPT++EHSKKAT +REG EEK+SM VKQT
Sbjct: 61  GDIIDCVSIYDQPAFDHPALRNHTIQLAPTYDPTIDEHSKKATAEREGKEEKNSMVVKQT 120

Query: 121 WRKSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSK 180
           WRKSGSCP+GTIP+RRIRK VLLKA+S+ERYGRK+P +S + AQL   ++SH LL NRSK
Sbjct: 121 WRKSGSCPEGTIPIRRIRKHVLLKAESIERYGRKKPMFSQENAQLPITRSSHTLLTNRSK 180

Query: 181 AFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYG 240
           AFL T G NYNAAKGDIKVCNP+VE DDEYSTSQVALL G YY+FE +ESGWAVNP VYG
Sbjct: 181 AFLLTAGNNYNAAKGDIKVCNPRVEFDDEYSTSQVALLTGPYYNFEAIESGWAVNPGVYG 240

Query: 241 DRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFR 300
           DRQTRLFVYWTADAS KTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEI +FLFR
Sbjct: 241 DRQTRLFVYWTADASEKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEIIMFLFR 300

Query: 301 DLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGR 360
           D +T+NWWVQYGESINIGYWP ELF ALSHTAETVQWGGEVYSTK+G PPHT T MG+GR
Sbjct: 301 DFKTDNWWVQYGESINIGYWPPELFSALSHTAETVQWGGEVYSTKIGRPPHTRTAMGSGR 360

Query: 361 FPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPG 420
           FPDF+ G SGWVKRMR+RDNSMVL FPGWVEHYSDEYDCYDVDF+RDYL+DPELYYGGPG
Sbjct: 361 FPDFISGTSGWVKRMRVRDNSMVLMFPGWVEHYSDEYDCYDVDFIRDYLDDPELYYGGPG 416

Query: 421 KNPKCP 426
           KNP+CP
Sbjct: 421 KNPRCP 416

BLAST of MC08g0520 vs. NCBI nr
Match: XP_022132062.1 (uncharacterized protein LOC111005024 [Momordica charantia] >XP_022132063.1 uncharacterized protein LOC111005024 [Momordica charantia])

HSP 1 Score: 717 bits (1852), Expect = 4.91e-260
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 87  MAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGTIPLRRIRKDVLLKAD 146
           MAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGTIPLRRIRKDVLLKAD
Sbjct: 1   MAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGTIPLRRIRKDVLLKAD 60

Query: 147 SVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSKAFLHTEGYNYNAAKGDIKVCNPKVES 206
           SVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSKAFLHTEGYNYNAAKGDIKVCNPKVES
Sbjct: 61  SVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSKAFLHTEGYNYNAAKGDIKVCNPKVES 120

Query: 207 DDEYSTSQVALLNGAYYHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCP 266
           DDEYSTSQVALLNGAYYHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCP
Sbjct: 121 DDEYSTSQVALLNGAYYHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCP 180

Query: 267 GFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFH 326
           GFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFH
Sbjct: 181 GFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFH 240

Query: 327 ALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKF 386
           ALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKF
Sbjct: 241 ALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKF 300

Query: 387 PGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP 426
           PGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP
Sbjct: 301 PGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP 340

BLAST of MC08g0520 vs. NCBI nr
Match: XP_022997646.1 (uncharacterized protein LOC111492515 isoform X1 [Cucurbita maxima])

HSP 1 Score: 722 bits (1863), Expect = 1.45e-259
Identity = 347/426 (81.46%), Postives = 379/426 (88.97%), Query Frame = 0

Query: 1   MGKRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSED 60
           MGKRF         MLV+TLTVIVCG VEGGSLSKQKSL    ++NSLRKQAIKSI+SED
Sbjct: 57  MGKRF------LLGMLVVTLTVIVCGAVEGGSLSKQKSL----RMNSLRKQAIKSIRSED 116

Query: 61  GDIIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQT 120
           GDIIDCV +Y+QPAFDHPAL NHTIQ+APTYDPT++EHSKKAT +REG EEK+SM VKQT
Sbjct: 117 GDIIDCVSIYDQPAFDHPALWNHTIQLAPTYDPTIDEHSKKATAEREGKEEKNSMVVKQT 176

Query: 121 WRKSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSK 180
           WRKSGSCP+GTIP+RRIRK VLLKA+SVERYGRK+P +S + AQL   ++SH LL NRSK
Sbjct: 177 WRKSGSCPEGTIPIRRIRKHVLLKAESVERYGRKKPMFSQENAQLPITRSSHTLLTNRSK 236

Query: 181 AFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYG 240
           AFL T G NYNAAKGDIKVCNP+VE DDEYSTSQVALL G YY+FE +ESGWAVNP VYG
Sbjct: 237 AFLLTAGNNYNAAKGDIKVCNPRVEFDDEYSTSQVALLTGPYYNFEAIESGWAVNPGVYG 296

Query: 241 DRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFR 300
           DRQTRLFVYWTADAS KTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEI +FLFR
Sbjct: 297 DRQTRLFVYWTADASQKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEIIMFLFR 356

Query: 301 DLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGR 360
           D +T+NWWVQYGESINIGYWP ELF ALSHTAETVQWGGEVYSTK+G PPHT T MG+GR
Sbjct: 357 DFKTDNWWVQYGESINIGYWPPELFGALSHTAETVQWGGEVYSTKIGRPPHTRTAMGSGR 416

Query: 361 FPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPG 420
           FPDF+ G SGWVKRMR+RDNSMVL FPGWVEHYSDEYDCYDVDF+RDYL+DPELYYGGPG
Sbjct: 417 FPDFISGTSGWVKRMRVRDNSMVLMFPGWVEHYSDEYDCYDVDFIRDYLDDPELYYGGPG 472

Query: 421 KNPKCP 426
           KNP+CP
Sbjct: 477 KNPRCP 472

BLAST of MC08g0520 vs. ExPASy TrEMBL
Match: A0A6J1BST2 (uncharacterized protein LOC111005024 OS=Momordica charantia OX=3673 GN=LOC111005024 PE=4 SV=1)

HSP 1 Score: 717 bits (1852), Expect = 2.38e-260
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 87  MAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGTIPLRRIRKDVLLKAD 146
           MAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGTIPLRRIRKDVLLKAD
Sbjct: 1   MAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGTIPLRRIRKDVLLKAD 60

Query: 147 SVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSKAFLHTEGYNYNAAKGDIKVCNPKVES 206
           SVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSKAFLHTEGYNYNAAKGDIKVCNPKVES
Sbjct: 61  SVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSKAFLHTEGYNYNAAKGDIKVCNPKVES 120

Query: 207 DDEYSTSQVALLNGAYYHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCP 266
           DDEYSTSQVALLNGAYYHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCP
Sbjct: 121 DDEYSTSQVALLNGAYYHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCP 180

Query: 267 GFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFH 326
           GFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFH
Sbjct: 181 GFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFH 240

Query: 327 ALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKF 386
           ALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKF
Sbjct: 241 ALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKF 300

Query: 387 PGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP 426
           PGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP
Sbjct: 301 PGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP 340

BLAST of MC08g0520 vs. ExPASy TrEMBL
Match: A0A6J1K820 (uncharacterized protein LOC111492515 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492515 PE=4 SV=1)

HSP 1 Score: 722 bits (1863), Expect = 7.01e-260
Identity = 347/426 (81.46%), Postives = 379/426 (88.97%), Query Frame = 0

Query: 1   MGKRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSED 60
           MGKRF         MLV+TLTVIVCG VEGGSLSKQKSL    ++NSLRKQAIKSI+SED
Sbjct: 57  MGKRF------LLGMLVVTLTVIVCGAVEGGSLSKQKSL----RMNSLRKQAIKSIRSED 116

Query: 61  GDIIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQT 120
           GDIIDCV +Y+QPAFDHPAL NHTIQ+APTYDPT++EHSKKAT +REG EEK+SM VKQT
Sbjct: 117 GDIIDCVSIYDQPAFDHPALWNHTIQLAPTYDPTIDEHSKKATAEREGKEEKNSMVVKQT 176

Query: 121 WRKSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSK 180
           WRKSGSCP+GTIP+RRIRK VLLKA+SVERYGRK+P +S + AQL   ++SH LL NRSK
Sbjct: 177 WRKSGSCPEGTIPIRRIRKHVLLKAESVERYGRKKPMFSQENAQLPITRSSHTLLTNRSK 236

Query: 181 AFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYG 240
           AFL T G NYNAAKGDIKVCNP+VE DDEYSTSQVALL G YY+FE +ESGWAVNP VYG
Sbjct: 237 AFLLTAGNNYNAAKGDIKVCNPRVEFDDEYSTSQVALLTGPYYNFEAIESGWAVNPGVYG 296

Query: 241 DRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFR 300
           DRQTRLFVYWTADAS KTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEI +FLFR
Sbjct: 297 DRQTRLFVYWTADASQKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEIIMFLFR 356

Query: 301 DLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGR 360
           D +T+NWWVQYGESINIGYWP ELF ALSHTAETVQWGGEVYSTK+G PPHT T MG+GR
Sbjct: 357 DFKTDNWWVQYGESINIGYWPPELFGALSHTAETVQWGGEVYSTKIGRPPHTRTAMGSGR 416

Query: 361 FPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPG 420
           FPDF+ G SGWVKRMR+RDNSMVL FPGWVEHYSDEYDCYDVDF+RDYL+DPELYYGGPG
Sbjct: 417 FPDFISGTSGWVKRMRVRDNSMVLMFPGWVEHYSDEYDCYDVDFIRDYLDDPELYYGGPG 472

Query: 421 KNPKCP 426
           KNP+CP
Sbjct: 477 KNPRCP 472

BLAST of MC08g0520 vs. ExPASy TrEMBL
Match: A0A6J1HDC5 (uncharacterized protein LOC111462577 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462577 PE=4 SV=1)

HSP 1 Score: 721 bits (1860), Expect = 9.22e-260
Identity = 344/426 (80.75%), Postives = 378/426 (88.73%), Query Frame = 0

Query: 1   MGKRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSED 60
           MGKRF         ML++TLTVIVCG VEGGSLSKQKS+    ++NSLRKQAIKSI+SED
Sbjct: 36  MGKRF------LLGMLIVTLTVIVCGAVEGGSLSKQKSM----RMNSLRKQAIKSIRSED 95

Query: 61  GDIIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQT 120
           GDIIDCV +Y+QPAFDHPALRNHTIQ+APTYDPT++EHSKKAT +REG EEK+SM VKQT
Sbjct: 96  GDIIDCVSIYDQPAFDHPALRNHTIQLAPTYDPTIDEHSKKATAEREGKEEKNSMVVKQT 155

Query: 121 WRKSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSK 180
           WRKSGSCP+GTIP+RRIRK VLLKA S+ERYGRK+P +S + AQL   ++SH LL NRSK
Sbjct: 156 WRKSGSCPEGTIPIRRIRKHVLLKAKSIERYGRKKPMFSQENAQLPITRSSHTLLTNRSK 215

Query: 181 AFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYG 240
           AFL T G NYNAAKGDIKVCNP+VE DDEYSTSQVALL G YY+FE +ESGWAVNP VYG
Sbjct: 216 AFLLTAGNNYNAAKGDIKVCNPRVEFDDEYSTSQVALLTGPYYNFEAIESGWAVNPGVYG 275

Query: 241 DRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFR 300
           DRQTRLFVYWTADAS KTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEI +FLFR
Sbjct: 276 DRQTRLFVYWTADASEKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEIIMFLFR 335

Query: 301 DLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGR 360
           D +T+NWWVQYGESINIGYWP ELF ALSHTAETVQWGGEVYST +G PPHT T MG+GR
Sbjct: 336 DFKTDNWWVQYGESINIGYWPPELFSALSHTAETVQWGGEVYSTNIGRPPHTRTAMGSGR 395

Query: 361 FPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPG 420
           FPDF+ G SGWVKRMR+RDNSMVL FPGWVEHYSDEYDCYDVDF+RDYL+DPELYYGGPG
Sbjct: 396 FPDFISGTSGWVKRMRVRDNSMVLMFPGWVEHYSDEYDCYDVDFIRDYLDDPELYYGGPG 451

Query: 421 KNPKCP 426
           KNP+CP
Sbjct: 456 KNPRCP 451

BLAST of MC08g0520 vs. ExPASy TrEMBL
Match: A0A0A0LQZ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G352360 PE=4 SV=1)

HSP 1 Score: 690 bits (1781), Expect = 3.24e-248
Identity = 334/426 (78.40%), Postives = 371/426 (87.09%), Query Frame = 0

Query: 1   MGKRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSED 60
           MGK   C+GL F KMLV  LTVIVCGVVE GSLSK K+    KK++SLRKQA KSIQSED
Sbjct: 1   MGKNVICNGLMF-KMLVGILTVIVCGVVEVGSLSKLKT----KKMSSLRKQATKSIQSED 60

Query: 61  GDIIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQT 120
           GDIIDCV +Y+QPAFDHPALRNHTIQMAPTYDPTM++HSKKAT + EGM EK SM VKQ 
Sbjct: 61  GDIIDCVSIYDQPAFDHPALRNHTIQMAPTYDPTMDKHSKKATAEEEGMGEKSSMGVKQP 120

Query: 121 WRKSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSK 180
           WRKSGSCPK TIP+RRIRK V LKA+SV  YG+KRPT   +IAQLSN+++SH LL N SK
Sbjct: 121 WRKSGSCPKETIPIRRIRKHVQLKANSVYSYGKKRPTPLLEIAQLSNSRSSHFLLKNHSK 180

Query: 181 AFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYG 240
           A L   G N+N AKGDIKVCNP VE DDEYSTSQVALL G YY++E +ESGWAVNP VYG
Sbjct: 181 AILLAVGDNFNGAKGDIKVCNPNVEFDDEYSTSQVALLTGPYYNYEAIESGWAVNPGVYG 240

Query: 241 DRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFR 300
           DRQTRLFVYWT DASHKTGCFDLTCPGFVQTSNEIALGSAIYPIST   LP+EIT+FLFR
Sbjct: 241 DRQTRLFVYWTVDASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTSTDLPFEITMFLFR 300

Query: 301 DLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGR 360
           D +TNNWWVQYGESINIGYWP+ELF AL +TAETVQWGGEVYSTKLGGPPHT TGMGNG+
Sbjct: 301 DFETNNWWVQYGESINIGYWPSELFKALKYTAETVQWGGEVYSTKLGGPPHTGTGMGNGK 360

Query: 361 FPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPG 420
           FPD++ G+SGWVKR+R+RDNSM+LKFP +VEHYSDEYDCYDVDF+R+YL+DPELYYGGPG
Sbjct: 361 FPDYISGDSGWVKRIRVRDNSMILKFPNFVEHYSDEYDCYDVDFIREYLDDPELYYGGPG 420

Query: 421 KNPKCP 426
           KN +CP
Sbjct: 421 KNWRCP 421

BLAST of MC08g0520 vs. ExPASy TrEMBL
Match: A0A5A7V3A1 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001210 PE=4 SV=1)

HSP 1 Score: 678 bits (1750), Expect = 1.47e-243
Identity = 330/426 (77.46%), Postives = 368/426 (86.38%), Query Frame = 0

Query: 1   MGKRFSCSGLRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSED 60
           MGK     GL F KMLV+ LTVIVCGVVE GSLSK +S    KK+NSLRKQA KSIQSED
Sbjct: 1   MGKNIIGDGLMF-KMLVVILTVIVCGVVEVGSLSKHES----KKMNSLRKQATKSIQSED 60

Query: 61  GDIIDCVRVYEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQT 120
           GDIIDCV +Y+QPAFDHPALRNHTIQMAPTYDPTM++HSKKAT ++EGM EK+SM VKQ 
Sbjct: 61  GDIIDCVSIYDQPAFDHPALRNHTIQMAPTYDPTMDKHSKKATAEKEGMGEKNSMGVKQP 120

Query: 121 WRKSGSCPKGTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVNRSK 180
           WR SGSCPK TIP+RRIRK     A+SV  YG+KRPT   +IAQLSN+++SH LL N SK
Sbjct: 121 WRNSGSCPKETIPIRRIRKH----ANSVYSYGKKRPTPLLEIAQLSNSRSSHFLLKNHSK 180

Query: 181 AFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPSVYG 240
           A L   G N+N AKGDIKVCNP VE DDEYSTSQVALL G YY++E +ESGWAVNP VYG
Sbjct: 181 AILLAVGNNFNGAKGDIKVCNPNVEFDDEYSTSQVALLTGPYYNYEAIESGWAVNPGVYG 240

Query: 241 DRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFR 300
           DRQTRLFVYWT DASHKTGCFDLTCPGFVQTSNEIALGSAIYPIST   LP+EIT+FLFR
Sbjct: 241 DRQTRLFVYWTIDASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTSAELPFEITMFLFR 300

Query: 301 DLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGR 360
           D +TNNWWVQYGESINIGYWP+ELF AL +TAETVQWGGEVYSTKLGGPPHT TGMGNG+
Sbjct: 301 DFETNNWWVQYGESINIGYWPSELFKALKYTAETVQWGGEVYSTKLGGPPHTATGMGNGK 360

Query: 361 FPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPG 420
           FPD++ G+SGWVKR+R+RDNSM+LKFP +VEHYSDEYDCYDVDF+R+YL+DPELYYGGPG
Sbjct: 361 FPDYISGDSGWVKRIRVRDNSMILKFPNFVEHYSDEYDCYDVDFIREYLDDPELYYGGPG 417

Query: 421 KNPKCP 426
           KN +CP
Sbjct: 421 KNWRCP 417

BLAST of MC08g0520 vs. TAIR 10
Match: AT5G25950.1 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 340.9 bits (873), Expect = 1.5e-93
Identity = 180/431 (41.76%), Postives = 261/431 (60.56%), Query Frame = 0

Query: 10  LRFFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSEDGDIIDCVRV 69
           +RFF      + VI+CG         + SL++  K+ +L K A+K+I+SEDGDIIDC+ +
Sbjct: 20  MRFF-----LIFVILCGFY--NEAYGKGSLDIDLKLKALNKPALKTIKSEDGDIIDCIDI 79

Query: 70  YEQPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPK 129
           Y+Q AFDHPAL+NH IQM     P+++  +KK T    G  E       Q W KSG CP 
Sbjct: 80  YKQHAFDHPALKNHKIQM----KPSVKFGTKKTTIPNNGSSEH---IKSQIWSKSGKCPM 139

Query: 130 GTIPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSN------NQTSHALLVN------ 189
           GTIP+RR+ ++ + +A S   +GRK P   HK + L N      N       +N      
Sbjct: 140 GTIPVRRVSREDISRASSPSHFGRKTP---HKYSFLDNALQHKGNFNITPAKINEAQPRL 199

Query: 190 RSKAFLHTEGYNYNAAKGDIKVCNPKVESDDEYSTSQVALLNGAYYHFECVESGWAVNPS 249
           RS+AF+   G+N+  A+ DI + NP      +YST+Q+ L+ G   +FE VE GW VNP+
Sbjct: 200 RSEAFIVALGFNFVGAQSDINIWNPPRVEATDYSTAQIWLVGGLSENFESVEGGWMVNPA 259

Query: 250 VYGDRQTRLFVYWTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLF 309
           V+GD +TRLF+ WT D   KTGC +L C GFVQTS + ALG+ + P+S+ +   Y IT+ 
Sbjct: 260 VFGDSRTRLFISWTTDGYTKTGCINLLCAGFVQTSKKFALGATVEPVSSSSSTQYHITVS 319

Query: 310 LFRDLQTNNWWVQYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLG-GPPHTTTGM 369
           +F D  + NWW+    ++ +GYWP  LF+ L H+A  VQWGGEV+S  +    PHTTT M
Sbjct: 320 IFLDPNSGNWWLTCENNV-LGYWPGTLFNYLKHSATAVQWGGEVHSPNVVLKKPHTTTAM 379

Query: 370 GNGRFPDFVFGNSGWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVR-DYLEDPELY 427
           G+G++  +++  + +   +RI+D SM LK+P ++  Y+DEY+CY     R  Y+ +P  Y
Sbjct: 380 GSGQWASYIWAEACFHTNLRIKDYSMQLKYPQFLSEYADEYNCYSTLLHRKTYMSEPHFY 432

BLAST of MC08g0520 vs. TAIR 10
Match: AT5G56530.1 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 323.6 bits (828), Expect = 2.5e-88
Identity = 166/399 (41.60%), Postives = 258/399 (64.66%), Query Frame = 0

Query: 32  SLSKQKSLEVVKKINSLRKQAIKSIQSEDGDIIDCVRVYEQPAFDHPALRNHTIQMAPTY 91
           S+S+Q + EV K +N L K A+KSIQS DGDIIDCV + +QPAFDHP L++H IQM P+Y
Sbjct: 34  SVSRQ-NFEVHKHLNRLNKPAVKSIQSPDGDIIDCVHISKQPAFDHPFLKDHKIQMGPSY 93

Query: 92  DPTMEEHSKKATEKREGMEEKDSM-AVKQTWRKSGSCPKGTIPLRRIRKDVLLKADSVER 151
            P       K +EK      K+S+  + Q W ++G C +GTIP+RR +K+ +L+A SV+R
Sbjct: 94  TPESLFGESKVSEK-----PKESVNPITQLWHQNGVCSEGTIPVRRTKKEDVLRASSVKR 153

Query: 152 YGRKRPTYSHKIAQLSNNQTSHALLVNRS---KAFLHTEGYNYNAAKGDIKVCNPKVESD 211
           YG+K+    H    L   +++   L+N+S    A  + EG  +  AK  I V  PKV+S 
Sbjct: 154 YGKKK----HLSVPLP--RSADPDLINQSGHQHAIAYVEGGKFYGAKATINVWEPKVQSS 213

Query: 212 DEYSTSQVALLNGAY-YHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCP 271
           +E+S SQ+ +L G++      +E+GW V+P +YGD  TRLF YWT+DA   TGC++L C 
Sbjct: 214 NEFSLSQLWILGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLCS 273

Query: 272 GFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFH 331
           GF+Q +++IA+G++I P+S  +   Y+I++ +++D +  +WW+Q+G+   +GYWP+ LF 
Sbjct: 274 GFIQINSQIAMGASISPVSGFHNPQYDISITIWKDPKEGHWWMQFGDGYVLGYWPSFLFS 333

Query: 332 ALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKF 391
            L+ +A  V+WGGEV + +  G  HTTT MG+G+FPD  F  + + + +++ D+S  LK 
Sbjct: 334 YLADSASIVEWGGEVVNMEEDG-HHTTTQMGSGQFPDEGFTKASYFRNIQVVDSSNNLKE 393

Query: 392 PGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKC 426
           P  +  ++++ +CYDV+  ++       YYGGPG+NP C
Sbjct: 394 PKGLNTFTEKSNCYDVEVGKNDDWGHYFYYGGPGRNPNC 419

BLAST of MC08g0520 vs. TAIR 10
Match: AT5G56530.2 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 323.6 bits (828), Expect = 2.5e-88
Identity = 166/399 (41.60%), Postives = 258/399 (64.66%), Query Frame = 0

Query: 32  SLSKQKSLEVVKKINSLRKQAIKSIQSEDGDIIDCVRVYEQPAFDHPALRNHTIQMAPTY 91
           S+S+Q + EV K +N L K A+KSIQS DGDIIDCV + +QPAFDHP L++H IQM P+Y
Sbjct: 34  SVSRQ-NFEVHKHLNRLNKPAVKSIQSPDGDIIDCVHISKQPAFDHPFLKDHKIQMGPSY 93

Query: 92  DPTMEEHSKKATEKREGMEEKDSM-AVKQTWRKSGSCPKGTIPLRRIRKDVLLKADSVER 151
            P       K +EK      K+S+  + Q W ++G C +GTIP+RR +K+ +L+A SV+R
Sbjct: 94  TPESLFGESKVSEK-----PKESVNPITQLWHQNGVCSEGTIPVRRTKKEDVLRASSVKR 153

Query: 152 YGRKRPTYSHKIAQLSNNQTSHALLVNRS---KAFLHTEGYNYNAAKGDIKVCNPKVESD 211
           YG+K+    H    L   +++   L+N+S    A  + EG  +  AK  I V  PKV+S 
Sbjct: 154 YGKKK----HLSVPLP--RSADPDLINQSGHQHAIAYVEGGKFYGAKATINVWEPKVQSS 213

Query: 212 DEYSTSQVALLNGAY-YHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCP 271
           +E+S SQ+ +L G++      +E+GW V+P +YGD  TRLF YWT+DA   TGC++L C 
Sbjct: 214 NEFSLSQLWILGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLCS 273

Query: 272 GFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFH 331
           GF+Q +++IA+G++I P+S  +   Y+I++ +++D +  +WW+Q+G+   +GYWP+ LF 
Sbjct: 274 GFIQINSQIAMGASISPVSGFHNPQYDISITIWKDPKEGHWWMQFGDGYVLGYWPSFLFS 333

Query: 332 ALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKF 391
            L+ +A  V+WGGEV + +  G  HTTT MG+G+FPD  F  + + + +++ D+S  LK 
Sbjct: 334 YLADSASIVEWGGEVVNMEEDG-HHTTTQMGSGQFPDEGFTKASYFRNIQVVDSSNNLKE 393

Query: 392 PGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKC 426
           P  +  ++++ +CYDV+  ++       YYGGPG+NP C
Sbjct: 394 PKGLNTFTEKSNCYDVEVGKNDDWGHYFYYGGPGRNPNC 419

BLAST of MC08g0520 vs. TAIR 10
Match: AT1G55360.1 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 322.0 bits (824), Expect = 7.2e-88
Identity = 161/398 (40.45%), Postives = 247/398 (62.06%), Query Frame = 0

Query: 33  LSKQKSLEVVKKINSLRKQAIKSIQSEDGDIIDCVRVYEQPAFDHPALRNHTIQMAPTYD 92
           +SKQK  EV K +N L K A+KSIQS DGD+IDCV + +QPAFDHP L++H IQM P Y 
Sbjct: 36  VSKQK-FEVKKHLNRLNKPAVKSIQSSDGDVIDCVPISKQPAFDHPFLKDHKIQMKPNYH 95

Query: 93  PTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGTIPLRRIRKDVLLKADSVERYG 152
           P       K +  +   +E     + Q W + G C +GTIP+RR ++D +L+A SV+RYG
Sbjct: 96  PEGLFDDNKVSAPKSNEKEGH---IPQLWHRYGKCSEGTIPMRRTKEDDVLRASSVKRYG 155

Query: 153 RKRPTYSHKIAQLSNNQTSHALLVNRS---KAFLHTEGYNYNAAKGDIKVCNPKVESDDE 212
           +K      K   +   +++   L+N+S    A  + EG  Y  AK  I V  PK++  +E
Sbjct: 156 KK------KRRSVPLPKSAEPDLINQSGHQHAIAYVEGDKYYGAKATINVWEPKIQQQNE 215

Query: 213 YSTSQVALLNGAY-YHFECVESGWAVNPSVYGDRQTRLFVYWTADASHKTGCFDLTCPGF 272
           +S SQ+ LL G++      +E+GW V+P +YGD  TRLF YWT+DA   TGC++L C GF
Sbjct: 216 FSLSQIWLLGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLCSGF 275

Query: 273 VQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWVQYGESINIGYWPAELFHAL 332
           +Q +++IA+G++I P+S      Y+I++ +++D +  +WW+Q+G    +GYWP+ LF  L
Sbjct: 276 IQINSDIAMGASISPVSGYRNSQYDISILIWKDPKEGHWWMQFGNGYVLGYWPSFLFSYL 335

Query: 333 SHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNSGWVKRMRIRDNSMVLKFPG 392
           + +A  ++WGGEV +++  G  HT+T MG+G+FP+  F  + + + +++ D S  LK P 
Sbjct: 336 TESASMIEWGGEVVNSQSDG-QHTSTQMGSGKFPEEGFSKASYFRNIQVVDGSNNLKAPK 395

Query: 393 WVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP 427
            +  ++++ +CYDV    +       YYGGPGKN KCP
Sbjct: 396 GLGTFTEQSNCYDVQTGSNDDWGHYFYYGGPGKNQKCP 422

BLAST of MC08g0520 vs. TAIR 10
Match: AT2G44210.1 (Protein of Unknown Function (DUF239) )

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-87
Identity = 165/417 (39.57%), Postives = 247/417 (59.23%), Query Frame = 0

Query: 12  FFKMLVLTLTVIVCGVVEGGSLSKQKSLEVVKKINSLRKQAIKSIQSEDGDIIDCVRVYE 71
           FF  LV+T+ ++   VV G   +    L++   +  L K A+KSI+S DGD+IDCV + +
Sbjct: 7   FFLALVMTVVILAPSVVSGE--NGFSDLKIRTHLKRLNKPALKSIKSPDGDMIDCVPITD 66

Query: 72  QPAFDHPALRNHTIQMAPTYDPTMEEHSKKATEKREGMEEKDSMAVKQTWRKSGSCPKGT 131
           QPAF HP L NHT+QM P+ +P       K + K +  +   S A+ Q W  +G CPK T
Sbjct: 67  QPAFAHPLLINHTVQMWPSLNPESVFSESKVSSKTKNQQ---SNAIHQLWHVNGKCPKNT 126

Query: 132 IPLRRIRKDVLLKADSVERYGRKRPTYSHKIAQLSNNQTSHALLVN-RSKAFLHTEGYNY 191
           IP+RR R+  L +A SVE YG K       I +  +++  + L  N    A ++ E   +
Sbjct: 127 IPIRRTRRQDLYRASSVENYGMKN---QKSIPKPKSSEPPNVLTQNGHQHAIMYVEDGVF 186

Query: 192 NAAKGDIKVCNPKVESDDEYSTSQVALLNGAY-YHFECVESGWAVNPSVYGDRQTRLFVY 251
             AK  I V  P VE  +E+S +Q+ +L G +      +E+GW V+P +YGD +TRLF Y
Sbjct: 187 YGAKAKINVWKPDVEMPNEFSLAQIWVLGGNFNSDLNSIEAGWQVSPQLYGDNRTRLFTY 246

Query: 252 WTADASHKTGCFDLTCPGFVQTSNEIALGSAIYPISTPNGLPYEITLFLFRDLQTNNWWV 311
           WT+DA   TGC++L C GFVQ + EIA+G +I P+S      Y+IT+ +++D +  +WW+
Sbjct: 247 WTSDAYQGTGCYNLLCSGFVQINREIAMGGSISPLSNYGNSQYDITILIWKDPKEGHWWL 306

Query: 312 QYGESINIGYWPAELFHALSHTAETVQWGGEVYSTKLGGPPHTTTGMGNGRFPDFVFGNS 371
           Q+GE   IGYWPA LF  LS +A  ++WGGEV +++     HTTT MG+GRF +  +G +
Sbjct: 307 QFGEKYIIGYWPASLFSYLSESASMIEWGGEVVNSQSEEGQHTTTQMGSGRFAEEGWGKA 366

Query: 372 GWVKRMRIRDNSMVLKFPGWVEHYSDEYDCYDVDFVRDYLEDPELYYGGPGKNPKCP 427
            + K +++ D S  L+ P  ++ ++D+ +CY+V            YYGGPG+NP CP
Sbjct: 367 SYFKNVQVVDGSNELRNPENLQVFTDQENCYNVKSGNGGSWGSYFYYGGPGRNPNCP 415

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_038886336.11.75e-26882.78uncharacterized protein LOC120076548 [Benincasa hispida][more]
XP_023546232.11.55e-26181.46uncharacterized protein LOC111805388 isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG7029277.18.95e-26181.22hypothetical protein SDJN02_07615, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022132062.14.91e-260100.00uncharacterized protein LOC111005024 [Momordica charantia] >XP_022132063.1 uncha... [more]
XP_022997646.11.45e-25981.46uncharacterized protein LOC111492515 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1BST22.38e-260100.00uncharacterized protein LOC111005024 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1K8207.01e-26081.46uncharacterized protein LOC111492515 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1HDC59.22e-26080.75uncharacterized protein LOC111462577 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0LQZ63.24e-24878.40Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G352360 PE=4 SV=1[more]
A0A5A7V3A11.47e-24377.46Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
Match NameE-valueIdentityDescription
AT5G25950.11.5e-9341.76Protein of Unknown Function (DUF239) [more]
AT5G56530.12.5e-8841.60Protein of Unknown Function (DUF239) [more]
AT5G56530.22.5e-8841.60Protein of Unknown Function (DUF239) [more]
AT1G55360.17.2e-8840.45Protein of Unknown Function (DUF239) [more]
AT2G44210.11.2e-8739.57Protein of Unknown Function (DUF239) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025521Neprosin activation peptidePFAMPF14365Neprosin_APcoord: 55..173
e-value: 3.7E-36
score: 124.2
NoneNo IPR availableGENE3D3.90.1320.10coord: 196..314
e-value: 2.7E-18
score: 68.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 93..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 94..114
NoneNo IPR availablePANTHERPTHR31589PROTEIN, PUTATIVE (DUF239)-RELATED-RELATEDcoord: 49..424
NoneNo IPR availablePANTHERPTHR31589:SF111CARBOXYL-TERMINAL PEPTIDASEcoord: 49..424
IPR004314NeprosinPFAMPF03080Neprosincoord: 197..419
e-value: 9.7E-71
score: 237.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g0520.1MC08g0520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane