MC07g0660 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0660
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionChlorophyllase
LocationMC07: 14489852 .. 14492318 (-)
RNA-Seq ExpressionMC07g0660
SyntenyMC07g0660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGTAAGGGGCATTTACGGAAACTTGAGTGTCATTGAAAATGGTTTCAAAATTCCATAAATATGGAGATAAAGAGACTGTGACCCACCCAGAGAGACTTCCGCGATGGCTATGGCTTCCTTGTCTTTTCATCCGCCGTCGGTCACTGTCGCCGGCGGCTCTGCAACTGCAACTGCGGGGAACGTATTTGAAATGGGAAAGTTCACCGTCGGCCCGATCGAGGAGGTTGGATCGGCCAGTACTTGTTGTCTTAATTGCGCCGGCAGATTTGCTCCGCCGAAGCCGGTGGTGATCGGCCACCCGGAGGAGGACGGCGAGTTTCCGGTGGTGCTGCTCATGCACGGCTACCTTCTTTGCAACACTTTCTACAGGCAGCTCATCCGACACATCGTTTCCCACGGCTTCATCGTCGTCGCTCCTCAGGTTCTTTCTATTTCTCACCACTCTCTTCTTCTTCTTCCAATTTCTTCATTACATTACAAATTTTGGTCGAAAGAATTTGAAATTTTTTCCCCAATTTATCCACAAACATTTTTTTAAAACCGACGCGGGGATTGAAATCCCATCATATTTCGATGTTTTAATAGTATAAGATATAGCCTGAATTCGAAATTCAAATTCACGATTTCCTGATTTAAAGACATTAAAAAATTAAAAAATAAGTGCCGCTGGTTTAGTTTTTTAGTTTATGCTCATAAAATAATTTAGTTTTAAAAACAAATTTAAAAAACTAATGCATATTTAAAAGTAATAACAAAATACACCTAGATTTCAAAATTAAATTTTGAAGGCTTCCCTTTTCTGAATATATTCTTTTATAAGTTTATTTCGTTTTTTATTGACTTTATCTTTATCTATATTTAATGGTTGAGAATGATAAACAAAAAATATCTCTATCTATATTTAATGGTTGAGAATGATAAATCTTCCTTTTAATTGAAATCTAAAAATCTACGACATAGCTTCCACAAAAAACGTTTTTCTTTTAAAGTATTCATAGAAATTCTAAACTGGTAATTCAAGTACGTGATTGGTAAATAAAATAGAAATACACGTTTGCTAATCATTCCTTTCTCATTCTGTAAAAATTGAAATTAATATATATATATATATATATATATATATGAAAATTAGTTTTTAAATTATTTATTATAATTTTTTATCCCATTTTTATTTTGTAAAAAAAAATATTGGATTGAGTTTTATGTGTTGATTATTTAATCAATAATTGTTTATCCCATTTTGACATCATTCTTTTTAAATAAAATGATGTATTTGAAAATTTACCATATAAAAGTGAGAAACCAAAAAGTCTGTTCAATATTTCTAATTTTTTCAAAAAATCTAAACATTTTGCAAGTCTTTCTTTTGTTCATTTTTATCACTTGAATTCAGAACTATGCCATTCATTTTTTTTTAACAAAAAATAAAAAATCAGAAACCCGAGCTAAATGTAATACTCCTTAATCCTAGAACTGATACATGGAAATGTCGTACAGTTATACACAGTAACGTGCCAAGATACAGGGGCCGAGATCAAAGCACTAGCGGCCATCGGCGAGTGGCTGCCGGAGGGGCTCCGGCATTATCTTCCGGCGAAGGTGAGCCCAAATCTGAAGAAACTGGCGCTGGCCGGCCACAGCCGCGGCGGCAAGGTCGCCTTCGCGTTTGCCCTAGCCCTAAACAAATCAACATCCAGCACAGCCCCAAAAGTGTCGGCCATAATCGGGCTCGACCCGGTCGACGGATCCAGGCCCCCAATCCTGACGGGCGTTTCAGAGTCCTTCGATTTCGTGTCCGCGATGCCGGTTCTGGTAATCGGGTCGGGCCTGGGGGAATTGCAGAGGAATCCTCTGTTCCCCCCCTGCGGTCCGAAGGGGGTGAATCACGAGGAGTTTTTCAAGGAGTGTCGGAGTCCGGCGCATTACATGGTGGTGAAGGATTACGGGCATATGGATTTGTTGGATGATGAGACGAGGGGAATTAGAGGGAAGGGGAGTTACTGTATGTGTAAAAATGGGGAATCGCGAGGGCCGATGAGGAGCTTTGTGGGTGGGGCTGTGGTGGCGTTTTTGAAGGCTTATTTGGAGGGGAAAGAGGAAGATTTGAGAGCCATTGAAGATGGGTCTTTGAGCTTGCCTGTACAGCTTCAGACTCTCCAAGTTCGTCTCTGAAAATCCACTCTCCAATCCAGAAGGTGGAGGCGGGGGATCGACCAGGGACGTATATGTTCAACTACGCTCGTTTTGGCTCAATCTACTGTATTGTTAGGCCTATTTCTATCTCATGTTTTCGTGATTTATTTTGGTCCATGTATTTTACACCTTTTTATTATGAATTCATATTTATATTTCAATAAGTATATTGGTTTATTGTGAATTAGTGGATATGTTAAAATACTAGTTTGGTGTCTGTATTTTCAATTTTTGTTTATTTTGGTCATTATACTTTCAAAATGTTCATTTTGG

mRNA sequence

GGGTAAGGGGCATTTACGGAAACTTGAGTGTCATTGAAAATGGTTTCAAAATTCCATAAATATGGAGATAAAGAGACTGTGACCCACCCAGAGAGACTTCCGCGATGGCTATGGCTTCCTTGTCTTTTCATCCGCCGTCGGTCACTGTCGCCGGCGGCTCTGCAACTGCAACTGCGGGGAACGTATTTGAAATGGGAAAGTTCACCGTCGGCCCGATCGAGGAGGTTGGATCGGCCAGTACTTGTTGTCTTAATTGCGCCGGCAGATTTGCTCCGCCGAAGCCGGTGGTGATCGGCCACCCGGAGGAGGACGGCGAGTTTCCGGTGGTGCTGCTCATGCACGGCTACCTTCTTTGCAACACTTTCTACAGGCAGCTCATCCGACACATCGTTTCCCACGGCTTCATCGTCGTCGCTCCTCAGTTATACACAGTAACGTGCCAAGATACAGGGGCCGAGATCAAAGCACTAGCGGCCATCGGCGAGTGGCTGCCGGAGGGGCTCCGGCATTATCTTCCGGCGAAGGTGAGCCCAAATCTGAAGAAACTGGCGCTGGCCGGCCACAGCCGCGGCGGCAAGGTCGCCTTCGCGTTTGCCCTAGCCCTAAACAAATCAACATCCAGCACAGCCCCAAAAGTGTCGGCCATAATCGGGCTCGACCCGGTCGACGGATCCAGGCCCCCAATCCTGACGGGCGTTTCAGAGTCCTTCGATTTCGTGTCCGCGATGCCGGTTCTGGTAATCGGGTCGGGCCTGGGGGAATTGCAGAGGAATCCTCTGTTCCCCCCCTGCGGTCCGAAGGGGGTGAATCACGAGGAGTTTTTCAAGGAGTGTCGGAGTCCGGCGCATTACATGGTGGTGAAGGATTACGGGCATATGGATTTGTTGGATGATGAGACGAGGGGAATTAGAGGGAAGGGGAGTTACTGTATGTGTAAAAATGGGGAATCGCGAGGGCCGATGAGGAGCTTTGTGGGTGGGGCTGTGGTGGCGTTTTTGAAGGCTTATTTGGAGGGGAAAGAGGAAGATTTGAGAGCCATTGAAGATGGGTCTTTGAGCTTGCCTGTACAGCTTCAGACTCTCCAAGTTCGTCTCTGAAAATCCACTCTCCAATCCAGAAGGTGGAGGCGGGGGATCGACCAGGGACGTATATGTTCAACTACGCTCGTTTTGGCTCAATCTACTGTATTGTTAGGCCTATTTCTATCTCATGTTTTCGTGATTTATTTTGGTCCATGTATTTTACACCTTTTTATTATGAATTCATATTTATATTTCAATAAGTATATTGGTTTATTGTGAATTAGTGGATATGTTAAAATACTAGTTTGGTGTCTGTATTTTCAATTTTTGTTTATTTTGGTCATTATACTTTCAAAATGTTCATTTTGG

Coding sequence (CDS)

ATGGCTATGGCTTCCTTGTCTTTTCATCCGCCGTCGGTCACTGTCGCCGGCGGCTCTGCAACTGCAACTGCGGGGAACGTATTTGAAATGGGAAAGTTCACCGTCGGCCCGATCGAGGAGGTTGGATCGGCCAGTACTTGTTGTCTTAATTGCGCCGGCAGATTTGCTCCGCCGAAGCCGGTGGTGATCGGCCACCCGGAGGAGGACGGCGAGTTTCCGGTGGTGCTGCTCATGCACGGCTACCTTCTTTGCAACACTTTCTACAGGCAGCTCATCCGACACATCGTTTCCCACGGCTTCATCGTCGTCGCTCCTCAGTTATACACAGTAACGTGCCAAGATACAGGGGCCGAGATCAAAGCACTAGCGGCCATCGGCGAGTGGCTGCCGGAGGGGCTCCGGCATTATCTTCCGGCGAAGGTGAGCCCAAATCTGAAGAAACTGGCGCTGGCCGGCCACAGCCGCGGCGGCAAGGTCGCCTTCGCGTTTGCCCTAGCCCTAAACAAATCAACATCCAGCACAGCCCCAAAAGTGTCGGCCATAATCGGGCTCGACCCGGTCGACGGATCCAGGCCCCCAATCCTGACGGGCGTTTCAGAGTCCTTCGATTTCGTGTCCGCGATGCCGGTTCTGGTAATCGGGTCGGGCCTGGGGGAATTGCAGAGGAATCCTCTGTTCCCCCCCTGCGGTCCGAAGGGGGTGAATCACGAGGAGTTTTTCAAGGAGTGTCGGAGTCCGGCGCATTACATGGTGGTGAAGGATTACGGGCATATGGATTTGTTGGATGATGAGACGAGGGGAATTAGAGGGAAGGGGAGTTACTGTATGTGTAAAAATGGGGAATCGCGAGGGCCGATGAGGAGCTTTGTGGGTGGGGCTGTGGTGGCGTTTTTGAAGGCTTATTTGGAGGGGAAAGAGGAAGATTTGAGAGCCATTGAAGATGGGTCTTTGAGCTTGCCTGTACAGCTTCAGACTCTCCAAGTTCGTCTCTGA

Protein sequence

MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKPVVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIKALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSAIIGLDPVDGSRPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQVRL
Homology
BLAST of MC07g0660 vs. ExPASy Swiss-Prot
Match: Q9M7I7 (Chlorophyllase-2 OS=Arabidopsis thaliana OX=3702 GN=CLH2 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.9e-93
Identity = 170/318 (53.46%), Postives = 228/318 (71.70%), Query Frame = 0

Query: 20  ATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRF--APPKPVVIGHPEEDGEFPVVLL 79
           +++++ N FE GK+    +    S+  C +  + R   +PPK +++  P E+G++PVV+L
Sbjct: 2   SSSSSRNAFEDGKYKSNLLTLDSSSRCCKITPSSRASPSPPKQLLVATPVEEGDYPVVML 61

Query: 80  MHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIKALAAIGEWLPEGLRHYL 139
           +HGYLL N+FY QL+ H+ SHGFI++APQLY++   DT  EIK+ A I +WL  GL H+L
Sbjct: 62  LHGYLLYNSFYSQLMLHVSSHGFILIAPQLYSIAGPDTMDEIKSTAEIMDWLSVGLNHFL 121

Query: 140 PAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSAIIGLDPVDG------SR 199
           PA+V+PNL K AL+GHSRGGK   AFA+AL K   S+  K+S +IG+DPVDG      + 
Sbjct: 122 PAQVTPNLSKFALSGHSRGGKT--AFAVALKKFGYSSNLKISTLIGIDPVDGTGKGKQTP 181

Query: 200 PPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFFKECRSPAHYMV 259
           PP+L  +  SFD +   P+LVIGSGLGE  RNPLFPPC P GVNH EFF+EC+ PA + V
Sbjct: 182 PPVLAYLPNSFD-LDKTPILVIGSGLGETARNPLFPPCAPPGVNHREFFRECQGPAWHFV 241

Query: 260 VKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKAYLEGKEEDLRA 319
            KDYGH+D+LDD+T+GIRGK SYC+CKNGE R PMR FVGG VV+FLKAYLEG + +L  
Sbjct: 242 AKDYGHLDMLDDDTKGIRGKSSYCLCKNGEERRPMRRFVGGLVVSFLKAYLEGDDRELVK 301

Query: 320 IEDG-SLSLPVQLQTLQV 329
           I+DG    +PV++Q  +V
Sbjct: 302 IKDGCHEDVPVEIQEFEV 316

BLAST of MC07g0660 vs. ExPASy Swiss-Prot
Match: O22527 (Chlorophyllase-1 OS=Arabidopsis thaliana OX=3702 GN=CLH1 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.0e-58
Identity = 134/301 (44.52%), Postives = 180/301 (59.80%), Query Frame = 0

Query: 28  FEMGKF--TVGPIEEVGSASTCCLNCAGRFAPPKPVVIGHPEEDGEFPVVLLMHGYLLCN 87
           FE+G    T  P++ V + ST         APPKPV I  P   G +PVVL  HG+ L N
Sbjct: 19  FEIGSLPTTEIPVDPVENDST---------APPKPVRITCPTVAGTYPVVLFFHGFYLRN 78

Query: 88  TFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIKALAAIGEWLPEGLRHYLPAKVSPNL 147
            FY  ++ HI SHG+I+VAPQL  +       E+    ++  W  E L+ +LP  V+ N 
Sbjct: 79  YFYSDVLNHIASHGYILVAPQLCKLLPPGGQVEVDDAGSVINWASENLKAHLPTSVNANG 138

Query: 148 KKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSAIIGLDPVDGSR------PPILTGVS 207
           K  +L GHSRGGK AFA AL  + +T   +   SA+IG+DPV G+       P ILT   
Sbjct: 139 KYTSLVGHSRGGKTAFAVALG-HAATLDPSITFSALIGIDPVAGTNKYIRTDPHILTYKP 198

Query: 208 ESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFFKECRSPAHYMVVKDYGHMD 267
           ESF+    +PV V+G+GLG  + N + PPC P  +NHEEF+KEC++   + V  DYGHMD
Sbjct: 199 ESFEL--DIPVAVVGTGLGP-KWNNVMPPCAPTDLNHEEFYKECKATKAHFVAADYGHMD 258

Query: 268 LLDDETRGIRGKGSYCMCKNGE-SRGPMRSFVGGAVVAFLKAYLEGKEEDLRAI-EDGSL 319
           +LDD+  G  G  + CMCKNG+  +  MRSFVGG VVAFLK  L G++ ++R I +D S+
Sbjct: 259 MLDDDLPGFVGFMAGCMCKNGQRKKSEMRSFVGGIVVAFLKYSLWGEKAEIRLIVKDPSV 306

BLAST of MC07g0660 vs. ExPASy Swiss-Prot
Match: Q94LX1 (Chlorophyllase-1, chloroplastic OS=Citrus unshiu OX=55188 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.9e-54
Identity = 141/338 (41.72%), Postives = 186/338 (55.03%), Query Frame = 0

Query: 3   MASLSFHPPSVTVAGGSATATAG-NVFEMGKFTVGPIE-EVGSASTCCLNCAGRFAPPKP 62
           MA++    P+ +V G    ATA   VF  G ++   I  E  S S+          PPKP
Sbjct: 1   MAAMVDSKPAASVQGTPLLATATLPVFTRGIYSTKRITLETSSPSSP--------PPPKP 60

Query: 63  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYT-VTCQDTGAEI 122
           ++I  P E G F V+L +HG  L N  Y ++  HI SHGFIVVAPQLYT +       E+
Sbjct: 61  LIIVTPAEKGTFNVILFLHGTSLSNKSYSKIFDHIASHGFIVVAPQLYTSIPPPSATNEL 120

Query: 123 KALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVS 182
            + A + EWLP+GL+  LP     N+  +A+ GHSRGG+ AFA +L              
Sbjct: 121 NSAAEVAEWLPQGLQQNLPENTEANVSLVAVMGHSRGGQTAFALSLRYG---------FG 180

Query: 183 AIIGLDPVDGSR------PPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKG 242
           A+IGLDPV G+       P IL+   +SFDF  ++PV VIG+GLG + R      C P+G
Sbjct: 181 AVIGLDPVAGTSKTTGLDPSILS--FDSFDF--SIPVTVIGTGLGGVAR--CITACAPEG 240

Query: 243 VNHEEFFKECR--SPAHYMVVKDYGHMDLLDDETRGIRGKG-SYCMCKNG-ESRGPMRSF 302
            NHEEFF  C+  S AH+ V  DYGHMD+LDD    ++    S   CKNG ESR PMR  
Sbjct: 241 ANHEEFFNRCKNSSRAHF-VATDYGHMDILDDNPSDVKSWALSKYFCKNGNESRDPMRRC 300

Query: 303 VGGAVVAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 328
           V G VVAFLK + +G  ED R I       P++L +++
Sbjct: 301 VSGIVVAFLKDFFDGDAEDFRQILKDPSFAPIKLDSVE 314

BLAST of MC07g0660 vs. ExPASy Swiss-Prot
Match: Q9MV14 (Chlorophyllase-1, chloroplastic OS=Citrus sinensis OX=2711 GN=CHLASE1 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 9.6e-53
Identity = 140/338 (41.42%), Postives = 184/338 (54.44%), Query Frame = 0

Query: 3   MASLSFHPPSVTVAGGSATATAG-NVFEMGKFTVGPIE-EVGSASTCCLNCAGRFAPPKP 62
           MA++    P+ +V G    ATA   VF  G ++   I  E  S S+          PPKP
Sbjct: 1   MAAMVDAKPAASVQGTPLLATATLPVFTRGIYSTKRITLETSSPSSP--------PPPKP 60

Query: 63  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYT-VTCQDTGAEI 122
           ++I  P   G F V+L +HG  L N  Y ++  HI SHGFIVVAPQLYT +       E+
Sbjct: 61  LIIVTPAGKGTFNVILFLHGTSLSNKSYSKIFDHIASHGFIVVAPQLYTSIPPPSATNEL 120

Query: 123 KALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVS 182
            + A + EWLP+GL+  LP     N+  +A+ GHSRGG+ AFA +L              
Sbjct: 121 NSAAEVAEWLPQGLQQNLPENTEANVSLVAVMGHSRGGQTAFALSLRYG---------FG 180

Query: 183 AIIGLDPVDGSR------PPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKG 242
           A+IGLDPV G+       P IL+   +SFDF  ++PV VIG+GLG + R      C P+G
Sbjct: 181 AVIGLDPVAGTSKTTGLDPSILS--FDSFDF--SIPVTVIGTGLGGVAR--CITACAPEG 240

Query: 243 VNHEEFFKECR--SPAHYMVVKDYGHMDLLDDETRGIRGKG-SYCMCKNG-ESRGPMRSF 302
            NHEEFF  C+  S AH+ V  DYGHMD+LDD    ++    S   CKNG ESR PMR  
Sbjct: 241 ANHEEFFNRCKNSSRAHF-VATDYGHMDILDDNPSDVKSWALSKYFCKNGNESRDPMRRC 300

Query: 303 VGGAVVAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 328
           V G VVAFLK +  G  ED R I       P++L +++
Sbjct: 301 VSGIVVAFLKDFFYGDAEDFRQILKDPSFAPIKLDSVE 314

BLAST of MC07g0660 vs. ExPASy Swiss-Prot
Match: Q9LE89 (Chlorophyllase type 0 OS=Chenopodium album OX=3559 GN=CACLH PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.7e-38
Identity = 102/271 (37.64%), Postives = 147/271 (54.24%), Query Frame = 0

Query: 55  FAPPKPVVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTV---T 114
           F+ P+P++I  P+E G +PV+L +HG +L N  Y     +I SHGFIVVAP+L+ +    
Sbjct: 57  FSAPEPLIIISPKEAGVYPVLLFIHGTMLSNEDYSLFFNYIASHGFIVVAPKLFRLFPPK 116

Query: 115 CQDTGAEIKALAAIGEWLPEGLRHYLP---AKVSPNLKKLALAGHSRGGKVAFAFALALN 174
                 EI   A++  W+P  L+  L      V  +L+KLA++GHSRGGK   AFALAL 
Sbjct: 117 LPSQQDEIDMAASVANWMPLYLQVVLQRYVTGVEGDLEKLAISGHSRGGK--SAFALALG 176

Query: 175 KSTSSTAPKVSAIIGLDPV------DGSRPPILTGVSESFDFVSAMPVLVIGSGLGELQR 234
            S        SA+IG+DPV      D + P +LT    SF+   ++PV VIGSGLG    
Sbjct: 177 FSNIKLDVTFSALIGVDPVAGRSVDDRTLPHVLTYKPNSFNL--SIPVTVIGSGLGNHTI 236

Query: 235 NPLFPPCGPKGVNHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMC-KNGE 294
           +     C P  V+H++F+ EC+  + + V+  YGHMD+L++            MC ++  
Sbjct: 237 S-----CAPNHVSHQQFYDECKENSSHFVITKYGHMDMLNEFRLSPIAVTMSLMCAQSFR 296

Query: 295 SRGPMRSFVGGAVVAFLKAYLEGKEEDLRAI 313
            +  MR  +GG +VAFL AY         AI
Sbjct: 297 PKATMRRTLGGIMVAFLNAYFRDDGRQYYAI 318

BLAST of MC07g0660 vs. NCBI nr
Match: XP_022138675.1 (chlorophyllase-2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 672 bits (1735), Expect = 4.89e-244
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP
Sbjct: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK
Sbjct: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA
Sbjct: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180

Query: 181 IIGLDPVDGSRPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFF 240
           IIGLDPVDGSRPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFF
Sbjct: 181 IIGLDPVDGSRPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFF 240

Query: 241 KECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKA 300
           KECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKA
Sbjct: 241 KECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKA 300

Query: 301 YLEGKEEDLRAIEDGSLSLPVQLQTLQVRL 330
           YLEGKEEDLRAIEDGSLSLPVQLQTLQVRL
Sbjct: 301 YLEGKEEDLRAIEDGSLSLPVQLQTLQVRL 330

BLAST of MC07g0660 vs. NCBI nr
Match: XP_022138435.1 (chlorophyllase-2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 458 bits (1179), Expect = 2.08e-159
Identity = 233/333 (69.97%), Postives = 266/333 (79.88%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMAS SF PPSVT    + +ATA NVFEMG F    + E   +  C     GRFAPPKP
Sbjct: 1   MAMASFSFLPPSVT----AGSATAANVFEMGSFNA--VMEKVESGFCG---GGRFAPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           VVIG P + GEFPV+LL+HGYLL NTFY QLI+HI SHGFIVVAPQLYTV   D+  EIK
Sbjct: 61  VVIGRPSDGGEFPVLLLIHGYLLYNTFYSQLIQHIASHGFIVVAPQLYTVAGPDSSGEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           A AAI +WLPEGLR +LP  V+PNL KLALAGHSRGGK +FAFALALNKS + TA K SA
Sbjct: 121 ATAAIADWLPEGLRQHLPPNVNPNLDKLALAGHSRGGKTSFAFALALNKSIT-TALKFSA 180

Query: 181 IIGLDPVDGS------RPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGV 240
           +IGLDPVDG+       PP+L  V +SFDF  A+PVLVIGSGLGE++RNPLFPPC PKG+
Sbjct: 181 LIGLDPVDGTGSGKQTHPPVLKFVPQSFDFGLALPVLVIGSGLGEVKRNPLFPPCAPKGL 240

Query: 241 NHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAV 300
           NHE+FF ECR+PAHY+VVKDYGH+DLLDDET GIRGK SYC+C+NGESRGPMR FVGGAV
Sbjct: 241 NHEDFFNECRNPAHYLVVKDYGHLDLLDDETGGIRGKASYCLCRNGESRGPMRRFVGGAV 300

Query: 301 VAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 327
           VAFLKAYL+G+E DL AIEDGS SLP+ LQT++
Sbjct: 301 VAFLKAYLDGEEGDLAAIEDGSRSLPLDLQTVK 323

BLAST of MC07g0660 vs. NCBI nr
Match: KAG7013529.1 (Chlorophyllase-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 422 bits (1085), Expect = 4.10e-145
Identity = 215/333 (64.56%), Postives = 253/333 (75.98%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMA+ SF PPS T    +A+AT+  VFE+GKF    +E+V SA  C         PPKP
Sbjct: 1   MAMATASFPPPSAT----AASATSPIVFEIGKFNA-VLEKVESAGCCSSGRHLPPPPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           ++IG P + GEFPV+LL+HGYLL NTFY QLI  I SHGFIV+APQLY+V   DT  EIK
Sbjct: 61  LLIGRPSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           A AA+  WLPEGLR +LP  V+PNL K+AL+GHSRGGK +FA ALAL +  S   PK+SA
Sbjct: 121 ATAAVINWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKS---PKLSA 180

Query: 181 IIGLDPVDGS------RPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGV 240
           +IGLDPVDG+       PP+L  +  SFD    +PVLVIGSGLGEL+RNPLFPPC PKG+
Sbjct: 181 LIGLDPVDGTGSGKQTHPPVLKYIPHSFDL--GVPVLVIGSGLGELKRNPLFPPCAPKGI 240

Query: 241 NHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAV 300
           NHEEFF+ECR+PAHY V KDYGH+DLLDDET G+RGK SYC+CKNGESR PMR FVGGAV
Sbjct: 241 NHEEFFEECRNPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAV 300

Query: 301 VAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 327
           VAFLKAYL G++ DLRAIEDG L LPV LQT++
Sbjct: 301 VAFLKAYLNGEDGDLRAIEDGDLCLPVHLQTVE 323

BLAST of MC07g0660 vs. NCBI nr
Match: XP_023548652.1 (chlorophyllase-2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421 bits (1083), Expect = 8.25e-145
Identity = 214/333 (64.26%), Postives = 253/333 (75.98%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMA+ SF PPS T    +A+AT+  VFE+GKF    +E+V SA  C         PPKP
Sbjct: 1   MAMATASFPPPSAT----AASATSSIVFEIGKFNA-VLEKVESAGCCSSGRHLPAPPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           ++IG P + GEF V+LL+HGYLL NTFY QLI  I SHGFIV+APQLY+V   DT  EIK
Sbjct: 61  LLIGRPSDAGEFQVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           A AA+  WLPEGLR +LP  V+PNL K+AL+GHSRGGK +FA ALAL +  S   PK+SA
Sbjct: 121 ATAAVINWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKS---PKLSA 180

Query: 181 IIGLDPVDGS------RPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGV 240
           +IGLDPVDG+       PP+L  + +SFD    +PVLVIGSGLGEL+RNPLFPPC PKG+
Sbjct: 181 LIGLDPVDGTGSGKQTHPPVLKYIPQSFDL--GVPVLVIGSGLGELKRNPLFPPCAPKGI 240

Query: 241 NHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAV 300
           NHEEFF+ECR+PAHY V KDYGH+DLLDDET G+RGK SYC+CKNGESR PMR FVGGAV
Sbjct: 241 NHEEFFEECRNPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAV 300

Query: 301 VAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 327
           VAFLKAYL G++ DLRAIEDG L LPV LQT++
Sbjct: 301 VAFLKAYLNGEDGDLRAIEDGDLCLPVHLQTVE 323

BLAST of MC07g0660 vs. NCBI nr
Match: XP_023006741.1 (chlorophyllase-2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 421 bits (1082), Expect = 1.17e-144
Identity = 214/333 (64.26%), Postives = 252/333 (75.68%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMA+ SF PPS T    + +AT+  VFE+GKF    +E+V SA  C         PPKP
Sbjct: 1   MAMATASFPPPSAT----AFSATSSIVFEIGKFNA-VLEKVESAGCCSSGRHLPAPPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           ++IG P   GEFPV+LL+HGYLL NTFY QLI  I SHGFIV+APQLY+V   DT  EIK
Sbjct: 61  LLIGRPSIAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           A AA+  WLPEGLR +LP  V+PNL K+AL+GHSRGGK +FA ALAL +  S   PK+SA
Sbjct: 121 ATAAVINWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKS---PKLSA 180

Query: 181 IIGLDPVDGS------RPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGV 240
           +IGLDPVDG+       PP+L  + +SFD    +PVLVIGSGLGEL+RNPLFPPC PKG+
Sbjct: 181 LIGLDPVDGTGSGKQTHPPVLKYIPQSFDL--GLPVLVIGSGLGELKRNPLFPPCAPKGI 240

Query: 241 NHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAV 300
           NHEEFFKECR+PAHY V KDYGH+DLLDDET G+RGK SYC+CKNG+SR PMR FVGGAV
Sbjct: 241 NHEEFFKECRNPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGDSREPMRRFVGGAV 300

Query: 301 VAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 327
           VAFLKAYL G++ DLRAIEDG L LPV LQT++
Sbjct: 301 VAFLKAYLNGEDGDLRAIEDGDLCLPVHLQTVE 323

BLAST of MC07g0660 vs. ExPASy TrEMBL
Match: A0A6J1CA40 (Chlorophyllase OS=Momordica charantia OX=3673 GN=LOC111009779 PE=3 SV=1)

HSP 1 Score: 672 bits (1735), Expect = 2.37e-244
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP
Sbjct: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK
Sbjct: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA
Sbjct: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180

Query: 181 IIGLDPVDGSRPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFF 240
           IIGLDPVDGSRPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFF
Sbjct: 181 IIGLDPVDGSRPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFF 240

Query: 241 KECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKA 300
           KECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKA
Sbjct: 241 KECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKA 300

Query: 301 YLEGKEEDLRAIEDGSLSLPVQLQTLQVRL 330
           YLEGKEEDLRAIEDGSLSLPVQLQTLQVRL
Sbjct: 301 YLEGKEEDLRAIEDGSLSLPVQLQTLQVRL 330

BLAST of MC07g0660 vs. ExPASy TrEMBL
Match: A0A6J1C9F9 (Chlorophyllase OS=Momordica charantia OX=3673 GN=LOC111009609 PE=3 SV=1)

HSP 1 Score: 458 bits (1179), Expect = 1.01e-159
Identity = 233/333 (69.97%), Postives = 266/333 (79.88%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMAS SF PPSVT    + +ATA NVFEMG F    + E   +  C     GRFAPPKP
Sbjct: 1   MAMASFSFLPPSVT----AGSATAANVFEMGSFNA--VMEKVESGFCG---GGRFAPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           VVIG P + GEFPV+LL+HGYLL NTFY QLI+HI SHGFIVVAPQLYTV   D+  EIK
Sbjct: 61  VVIGRPSDGGEFPVLLLIHGYLLYNTFYSQLIQHIASHGFIVVAPQLYTVAGPDSSGEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           A AAI +WLPEGLR +LP  V+PNL KLALAGHSRGGK +FAFALALNKS + TA K SA
Sbjct: 121 ATAAIADWLPEGLRQHLPPNVNPNLDKLALAGHSRGGKTSFAFALALNKSIT-TALKFSA 180

Query: 181 IIGLDPVDGS------RPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGV 240
           +IGLDPVDG+       PP+L  V +SFDF  A+PVLVIGSGLGE++RNPLFPPC PKG+
Sbjct: 181 LIGLDPVDGTGSGKQTHPPVLKFVPQSFDFGLALPVLVIGSGLGEVKRNPLFPPCAPKGL 240

Query: 241 NHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAV 300
           NHE+FF ECR+PAHY+VVKDYGH+DLLDDET GIRGK SYC+C+NGESRGPMR FVGGAV
Sbjct: 241 NHEDFFNECRNPAHYLVVKDYGHLDLLDDETGGIRGKASYCLCRNGESRGPMRRFVGGAV 300

Query: 301 VAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 327
           VAFLKAYL+G+E DL AIEDGS SLP+ LQT++
Sbjct: 301 VAFLKAYLDGEEGDLAAIEDGSRSLPLDLQTVK 323

BLAST of MC07g0660 vs. ExPASy TrEMBL
Match: A0A6J1KYL8 (Chlorophyllase OS=Cucurbita maxima OX=3661 GN=LOC111499382 PE=3 SV=1)

HSP 1 Score: 421 bits (1082), Expect = 5.67e-145
Identity = 214/333 (64.26%), Postives = 252/333 (75.68%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMA+ SF PPS T    + +AT+  VFE+GKF    +E+V SA  C         PPKP
Sbjct: 1   MAMATASFPPPSAT----AFSATSSIVFEIGKFNA-VLEKVESAGCCSSGRHLPAPPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           ++IG P   GEFPV+LL+HGYLL NTFY QLI  I SHGFIV+APQLY+V   DT  EIK
Sbjct: 61  LLIGRPSIAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           A AA+  WLPEGLR +LP  V+PNL K+AL+GHSRGGK +FA ALAL +  S   PK+SA
Sbjct: 121 ATAAVINWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKS---PKLSA 180

Query: 181 IIGLDPVDGS------RPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGV 240
           +IGLDPVDG+       PP+L  + +SFD    +PVLVIGSGLGEL+RNPLFPPC PKG+
Sbjct: 181 LIGLDPVDGTGSGKQTHPPVLKYIPQSFDL--GLPVLVIGSGLGELKRNPLFPPCAPKGI 240

Query: 241 NHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAV 300
           NHEEFFKECR+PAHY V KDYGH+DLLDDET G+RGK SYC+CKNG+SR PMR FVGGAV
Sbjct: 241 NHEEFFKECRNPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGDSREPMRRFVGGAV 300

Query: 301 VAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 327
           VAFLKAYL G++ DLRAIEDG L LPV LQT++
Sbjct: 301 VAFLKAYLNGEDGDLRAIEDGDLCLPVHLQTVE 323

BLAST of MC07g0660 vs. ExPASy TrEMBL
Match: A0A6J1H5Z8 (Chlorophyllase OS=Cucurbita moschata OX=3662 GN=LOC111459924 PE=3 SV=1)

HSP 1 Score: 421 bits (1081), Expect = 8.05e-145
Identity = 214/333 (64.26%), Postives = 253/333 (75.98%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMA+ SF PPS T    +A+AT+  VFE+GKF    +E+V SA  C         PPKP
Sbjct: 1   MAMATASFPPPSAT----AASATSPIVFEIGKFNA-VLEKVESAGCCSSGRHLPPPPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           ++I  P + GEFPV+LL+HGYLL NTFY QLI  I SHGFIV+APQLY+V   DT  EIK
Sbjct: 61  LLIARPSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           A AA+  WLPEGLR +LP  V+PNL K+AL+GHSRGGK +FA ALAL +  S   PK+SA
Sbjct: 121 ATAAVINWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKS---PKLSA 180

Query: 181 IIGLDPVDGS------RPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGV 240
           +IGLDPVDG+       PP+L  + +SFD    +PVLVIGSGLGEL+RNPLFPPC PKG+
Sbjct: 181 LIGLDPVDGTGSGKQTHPPVLKYIPQSFDL--GVPVLVIGSGLGELKRNPLFPPCAPKGI 240

Query: 241 NHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAV 300
           NHEEFF+ECR+PAHY V KDYGH+DLLDDET G+RGK SYC+CKNGESR PMR FVGGAV
Sbjct: 241 NHEEFFEECRNPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAV 300

Query: 301 VAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 327
           VAFLKAYL G++ DLRAIEDG L LPV LQT++
Sbjct: 301 VAFLKAYLNGEDGDLRAIEDGDLCLPVHLQTVE 323

BLAST of MC07g0660 vs. ExPASy TrEMBL
Match: A0A5A7UQQ1 (Chlorophyllase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001310 PE=3 SV=1)

HSP 1 Score: 412 bits (1059), Expect = 1.39e-141
Identity = 215/333 (64.56%), Postives = 248/333 (74.47%), Query Frame = 0

Query: 1   MAMASLSFHPPSVTVAGGSATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRFAPPKP 60
           MAMA+ SF PP  T    + +AT  NVFE+GKF    + E      CC +  GRF PPKP
Sbjct: 1   MAMATASFPPPLPT----AGSATLSNVFEIGKFNA--VLEKVEPGGCCSS--GRFLPPKP 60

Query: 61  VVIGHPEEDGEFPVVLLMHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIK 120
           ++IG P E GEFPV+LL+HGYLL NTFY QLI HI SHGFIVVAPQLYTV   D   EIK
Sbjct: 61  LLIGRPSEAGEFPVLLLVHGYLLYNTFYSQLIHHIASHGFIVVAPQLYTVAGPDASEEIK 120

Query: 121 ALAAIGEWLPEGLRHYLPAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSA 180
           A AA+  WLP GLR +LP  V+PNL K+ALAGHSRGGK +FA  LAL KS     PK+SA
Sbjct: 121 ATAAVINWLPTGLRQHLPPHVNPNLTKIALAGHSRGGKTSFA--LALQKS-----PKLSA 180

Query: 181 IIGLDPVDGS------RPPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGV 240
           +IGLDPVDG+       PP+L  + +S D    +PVLVIGSGLGEL+ NPLFPPC PKG+
Sbjct: 181 LIGLDPVDGTGSGKQTHPPVLKYIPQSLDL--GIPVLVIGSGLGELKMNPLFPPCAPKGI 240

Query: 241 NHEEFFKECRSPAHYMVVKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAV 300
           NHEEFFKECR+PA++ VVKDYGHMDLLDDET GIRGK SYC+CKNGESR PMR FVGGAV
Sbjct: 241 NHEEFFKECRNPAYHFVVKDYGHMDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAV 300

Query: 301 VAFLKAYLEGKEEDLRAIEDGSLSLPVQLQTLQ 327
           VAFLK+ L G+E DL+AIE G L LPV LQT++
Sbjct: 301 VAFLKSCLNGEEGDLKAIEGGDLILPVHLQTIE 316

BLAST of MC07g0660 vs. TAIR 10
Match: AT5G43860.1 (chlorophyllase 2 )

HSP 1 Score: 344.0 bits (881), Expect = 1.4e-94
Identity = 170/318 (53.46%), Postives = 228/318 (71.70%), Query Frame = 0

Query: 20  ATATAGNVFEMGKFTVGPIEEVGSASTCCLNCAGRF--APPKPVVIGHPEEDGEFPVVLL 79
           +++++ N FE GK+    +    S+  C +  + R   +PPK +++  P E+G++PVV+L
Sbjct: 2   SSSSSRNAFEDGKYKSNLLTLDSSSRCCKITPSSRASPSPPKQLLVATPVEEGDYPVVML 61

Query: 80  MHGYLLCNTFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIKALAAIGEWLPEGLRHYL 139
           +HGYLL N+FY QL+ H+ SHGFI++APQLY++   DT  EIK+ A I +WL  GL H+L
Sbjct: 62  LHGYLLYNSFYSQLMLHVSSHGFILIAPQLYSIAGPDTMDEIKSTAEIMDWLSVGLNHFL 121

Query: 140 PAKVSPNLKKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSAIIGLDPVDG------SR 199
           PA+V+PNL K AL+GHSRGGK   AFA+AL K   S+  K+S +IG+DPVDG      + 
Sbjct: 122 PAQVTPNLSKFALSGHSRGGKT--AFAVALKKFGYSSNLKISTLIGIDPVDGTGKGKQTP 181

Query: 200 PPILTGVSESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFFKECRSPAHYMV 259
           PP+L  +  SFD +   P+LVIGSGLGE  RNPLFPPC P GVNH EFF+EC+ PA + V
Sbjct: 182 PPVLAYLPNSFD-LDKTPILVIGSGLGETARNPLFPPCAPPGVNHREFFRECQGPAWHFV 241

Query: 260 VKDYGHMDLLDDETRGIRGKGSYCMCKNGESRGPMRSFVGGAVVAFLKAYLEGKEEDLRA 319
            KDYGH+D+LDD+T+GIRGK SYC+CKNGE R PMR FVGG VV+FLKAYLEG + +L  
Sbjct: 242 AKDYGHLDMLDDDTKGIRGKSSYCLCKNGEERRPMRRFVGGLVVSFLKAYLEGDDRELVK 301

Query: 320 IEDG-SLSLPVQLQTLQV 329
           I+DG    +PV++Q  +V
Sbjct: 302 IKDGCHEDVPVEIQEFEV 316

BLAST of MC07g0660 vs. TAIR 10
Match: AT1G19670.1 (chlorophyllase 1 )

HSP 1 Score: 227.6 bits (579), Expect = 1.4e-59
Identity = 134/301 (44.52%), Postives = 180/301 (59.80%), Query Frame = 0

Query: 28  FEMGKF--TVGPIEEVGSASTCCLNCAGRFAPPKPVVIGHPEEDGEFPVVLLMHGYLLCN 87
           FE+G    T  P++ V + ST         APPKPV I  P   G +PVVL  HG+ L N
Sbjct: 19  FEIGSLPTTEIPVDPVENDST---------APPKPVRITCPTVAGTYPVVLFFHGFYLRN 78

Query: 88  TFYRQLIRHIVSHGFIVVAPQLYTVTCQDTGAEIKALAAIGEWLPEGLRHYLPAKVSPNL 147
            FY  ++ HI SHG+I+VAPQL  +       E+    ++  W  E L+ +LP  V+ N 
Sbjct: 79  YFYSDVLNHIASHGYILVAPQLCKLLPPGGQVEVDDAGSVINWASENLKAHLPTSVNANG 138

Query: 148 KKLALAGHSRGGKVAFAFALALNKSTSSTAPKVSAIIGLDPVDGSR------PPILTGVS 207
           K  +L GHSRGGK AFA AL  + +T   +   SA+IG+DPV G+       P ILT   
Sbjct: 139 KYTSLVGHSRGGKTAFAVALG-HAATLDPSITFSALIGIDPVAGTNKYIRTDPHILTYKP 198

Query: 208 ESFDFVSAMPVLVIGSGLGELQRNPLFPPCGPKGVNHEEFFKECRSPAHYMVVKDYGHMD 267
           ESF+    +PV V+G+GLG  + N + PPC P  +NHEEF+KEC++   + V  DYGHMD
Sbjct: 199 ESFEL--DIPVAVVGTGLGP-KWNNVMPPCAPTDLNHEEFYKECKATKAHFVAADYGHMD 258

Query: 268 LLDDETRGIRGKGSYCMCKNGE-SRGPMRSFVGGAVVAFLKAYLEGKEEDLRAI-EDGSL 319
           +LDD+  G  G  + CMCKNG+  +  MRSFVGG VVAFLK  L G++ ++R I +D S+
Sbjct: 259 MLDDDLPGFVGFMAGCMCKNGQRKKSEMRSFVGGIVVAFLKYSLWGEKAEIRLIVKDPSV 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M7I71.9e-9353.46Chlorophyllase-2 OS=Arabidopsis thaliana OX=3702 GN=CLH2 PE=1 SV=1[more]
O225272.0e-5844.52Chlorophyllase-1 OS=Arabidopsis thaliana OX=3702 GN=CLH1 PE=1 SV=1[more]
Q94LX13.9e-5441.72Chlorophyllase-1, chloroplastic OS=Citrus unshiu OX=55188 PE=2 SV=1[more]
Q9MV149.6e-5341.42Chlorophyllase-1, chloroplastic OS=Citrus sinensis OX=2711 GN=CHLASE1 PE=1 SV=1[more]
Q9LE898.7e-3837.64Chlorophyllase type 0 OS=Chenopodium album OX=3559 GN=CACLH PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022138675.14.89e-244100.00chlorophyllase-2, chloroplastic-like [Momordica charantia][more]
XP_022138435.12.08e-15969.97chlorophyllase-2, chloroplastic-like [Momordica charantia][more]
KAG7013529.14.10e-14564.56Chlorophyllase-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023548652.18.25e-14564.26chlorophyllase-2, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_023006741.11.17e-14464.26chlorophyllase-2, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CA402.37e-244100.00Chlorophyllase OS=Momordica charantia OX=3673 GN=LOC111009779 PE=3 SV=1[more]
A0A6J1C9F91.01e-15969.97Chlorophyllase OS=Momordica charantia OX=3673 GN=LOC111009609 PE=3 SV=1[more]
A0A6J1KYL85.67e-14564.26Chlorophyllase OS=Cucurbita maxima OX=3661 GN=LOC111499382 PE=3 SV=1[more]
A0A6J1H5Z88.05e-14564.26Chlorophyllase OS=Cucurbita moschata OX=3662 GN=LOC111459924 PE=3 SV=1[more]
A0A5A7UQQ11.39e-14164.56Chlorophyllase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G00131... [more]
Match NameE-valueIdentityDescription
AT5G43860.11.4e-9453.46chlorophyllase 2 [more]
AT1G19670.11.4e-5944.52chlorophyllase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 52..316
e-value: 1.6E-26
score: 95.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 63..303
IPR017395ChlorophyllasePFAMPF07224Chlorophyllasecoord: 25..323
e-value: 6.4E-102
score: 340.7
NoneNo IPR availablePANTHERPTHR33428:SF13CHLOROPHYLLASE-2, CHLOROPLASTICcoord: 19..328
NoneNo IPR availablePANTHERPTHR33428CHLOROPHYLLASE-2, CHLOROPLASTICcoord: 19..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0660.1MC07g0660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015996 chlorophyll catabolic process
molecular_function GO:0047746 chlorophyllase activity
molecular_function GO:0102293 pheophytinase b activity