MC07g0386 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0386
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationMC07: 11708117 .. 11712418 (-)
RNA-Seq ExpressionMC07g0386
SyntenyMC07g0386
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGATCTTTTTCTCATTACCGTGTCTTTTAAGGGTGGAGATTTTGGGATTAAGCGGGCTAAAGTTGATCAAGAAACTGAACGGAAGGTTGATGGGGTATCGGATGATGTCGGAAGAATTGCCTCTGGATTAGAACCGAATTTGGCTTCTACTTCAATGGACAATGCTGCAAGCACATCGAATATTTCATCAACGGCTGGAGCAGTAAAGTCCGGCTATGATCACCTTCCAAGAGAAATGCGTGAAATGAGAATTAGAGATGACAAAACCAACCATGATGAAAAGGTTATTCAGAGAGACTTTTGCTTCTCAAATATAGCTCTTGTTTTGTTTTGTTATCTTCCTTTTTTAAACAATCTCTCCATCTTTTCAATATCTTTCATGCCACTTTCTCCATGTACATTTGCAATTAAGTTGCTAGTATCTTGTATTCTTTGATATGTAGTCCATGTGGAAAACGTAACTTGGATACTTCTGCAACGTGGTAAATCAAGTGGAAATAAGTATTGAAATTTTATCTGCTATGATCCTGTTTTAGGATTTGGTGGAACCTGCTATCATTAATGGCAGTGGAACAGAAGCTGGTCAGATAATAGCAACTACAGTAGGCGGGCGGAACGGAAAACCAAAACAGGTTACTTTTAATTAGTCTGCATATTGTTTGTCATTTGGTTGCACATATCCTCCTCTCTATATCTCCCACGTATGCTCATAATCTCAGAGGAAGCTATGAAATAACTTATCGTAACCTTTTCAATCAAATCATATGGTATTTGATTTCTTGTAATTGAGGTGGAGAGGTGGTTGAAAATTCTTTGCTGTTGTCACCTTGTTGCAGACGATCTCCTACATGGCAGAGCGTGTGGTTGGTACTGGTTCTTTCGGTGTTGTCTTCCAGGTATTTTAATATGCTGTTCCATGTTCAACCAATACTCCTTGCTCTGGTTCCTTTCAGATCCTGAGGTTATTGCAACTTTTAAAATCATATGAATGTTATTGTAATAGGCTAAGTGCTTGGAAACAAATGAAGCTGTAGCAATAAAGAAGGTGCTGCAGGATAAGAGATACAAGAACAGGGAACTTCAGATTATGCGCATACTTGACCATCCTAATGTCGTTCAACTGAAGCATTGTTTCTTTTCCACTACTGATAAAGATGAGCTGTACCTTAACCTTGTCCTGGAGTTTATATCTGAAACTGTCTACAGAGTTTCTAAGCATTATATCAGGATGAACCGAAGCATGCCCATTATTTATGTGCAGCTTTATACATATCAGGTAAACTTTTCAATTCGTGATGAATTACTGTTGATTTGATGACGTGAGAAGTATTTTAACCTTCAATCTGGAATTCTTGCTGAATTTGGCTGTTGCAATCCTATGTATAAATGTAGTAACTTATGTTCTCACTTCTCCAGATTTGCCGGGCTTTGAATTACTTGCATCATGTTGTAGGGGTGTGTCATCGTGATATTAAGCCTCAGAATTTACTGGTAGGTCTTTTTCCTTTTTTCCCATCATCTGACAGCAACTCCTTTTGTTACTTTTTTTTTAAATGAAAAACTGAACTTGGGCAAATAAAAAGGCAATCCAACAAAAGGAGACAAACCAAAACATGAACTAACTATTCAAAAAGGGCTCAAATTGAAAAGAACAAGGCCTAATGGATAATGACAAAAACTCCCTTAGATACTAATTTTTAAAGAGTCATTAAACCTGGCGTGAGACAAAACCTCTTCTCGGGACCTCTCAACCCCCTAAAGTTCCCATTATTCATCTCAAGTCAAATGCTCCAAAGAAAAAAAGGAACAAAAATGTTTGTTGTGAGCCAAAACAAACCCCAAGTGACTTCTAAAAACAGTCCTAGATGAAGTGGGCAAACTAATAGCTCCAAAGATTTGATCCAAACTTAATGTTCTTAGTTTATAAAAATACTATGTCCATTCAGACCTGAGGATTCACTTTTTCGAAAAATGTGCATACAGTTAGTATTCTTAAACATTTTACTAGGGGATTTAAATGAGAATGCACTACCTGCATTCCATTCCAGAAATTTATTTGCACGTGTATGGCTGCTTATACTACAGCCCAGTCGTCAAATGTTGGCTAAAATCACCCTCAGGACCTCAACAATATGTTTTCATGTTAATAGCTATTAAAGCACACATTGTATAAGCATATTGAAATGGTAAGAGACTTCAAGTCGATATGTATTATGATGTTGAATTAAAATGTATTTACATAGAAGACCATGTCATACTCTGAATACAGAATTAATGTGGATGGAGTCGATAGAAATGTCTCCAGAAAAATAGGTTCCTCAAGTTGAAAGTTAGAGTACGGTAGAGATGACAGGAGTTGTCTTTTATCTGCTGATCATTTTCAGCTGCTCAATCGTGCAGGTTAATCCTCACACCCATCAGCTGAAGATATGTGATTTTGGGAGTGCAAAGATGTTGGTATGCATTTACATCTGAATATGGATCTGATTCTTTTCTGATGAAATATATTGATTTATGATCTGATGCGATTAATTTTTCAATTTCAGATGCCTGGGGAACCTAACATATCTTATATTTGTTCACGGTATTATAGGGCCCCAGAACTCATATTTGGGGCAACAGAGTATACCAATGCAATTGATATGTGGTCTGTAGGTTGTGTAATGGCTGAGCTTCTTTTAGGACAGGTAGGTCTTCATCTGAATTGCTGCCTTGAATATAAAGTTGTAGCTTGTAGGCCATTATCTAACTCTTATGGACTTTTGTGGTTCATGTAAAAACTGTCAACTGTGCATAGACTTCATAATCTTTTCATCATATGTTCATTGAGAATCTGTTTTTTCGGTGTAGCGTGGTTTATGCCGAAAAGAAGATTATTTAAATTGTTGAGTTATTTCTGTATCATCTTCTTGCTCTTTCTGATGTGCCATGGACATGTAAATTATTTGCAGCCTCTTTTTCCTGGTGAAAGTGGTGTTGATCAATTGGTAGAAATCATCAAGGTACAACCGATCTGTTGAATAATATCGATATCAAAAGAAAGTTGGGACTTATTCCTTTTTTCTGTTTCACTTTTCTAATTTTCTCTCATGGCTTTATGTCTCCTTTCTTTCACCCTCAGATATTGGGAACACCAACCAGAGAGGAAATTAAGTGCATGAACCCAAATTATACAGAATTCAAATTTCCTCAGATTAAAGCGCATCCATGGCATAAGGTCTCTTGCATTTTCATCAGAAAATTTATTCTTTTTTTTTTCCTCAGTCATGTTTGTTTACTACTATTAGTATTGTCATGAGGGTGGAAATCTTATTGAAGCTATAATCCCAGATATTTCACTCGAGAATGCCTGCCGAAGCAATCGATCTTGTATCTAGATTGCTCCAGTATTCGCCCAACCTACGTTGTACTGCGGTAAAAAACTTTACTCATTTAATGATGACTTTGTTTCTTTGAAATCACAAAAATTATTATTCCCATTTCTATGCCGCTTTCTAGGACAATATGATTATTTTTTAAATCATATTCTAATGTTTTGTTGTTTTGCAACGTGTGATGCTGCTGCTGCTATGAGTCAAATGTTTCTAGTAGTTCTAATGCCTCGTAGCAAGAACTCTATCACCAATATAGATGGATTTCTATGCCCATAGATAAGATTAGGGAAAATTACATTAGCACCTGAGTTTTACTCTTCTTTTGTCCTTGAATTTTCAAAATGCATGATTTAACCTTGAAATCGCACTAAATAGTATCATTATGAGAGATACCAGAGGTGTAAAAAGTAACTGTAGTGAAATTATTTCATAGAACTCTGGAATTATATCATATGCTTTGAAAATTTAGGAACAAGTGAAAGTTCAGAGACTACTTAGATGTCTATCGCGACACGTTCTGCCTGTTAATTATATTGTCAATGCTATCATTTTGATCATAATATCACATTGAAATGTGACACTCATAGTCTAGCAAGGAACTCATAATTTTATCATATGGTTGCTTATGGTCAATCTAACCATTAAGTGGCCTTTTTTTCTCTCCCTTCAGTTGGAGGCTTGTGCACACCCTTTCTTTGACGATTTAAGAGATCCAAATGCATGTTTACCCAACGGCCAAGCTCTACCGCCATTGTTCAATTTCACAGCACAAGGTAAAACCTAACTATTCAACATCATGTTCGACGCTTTGACAAGACTGCTTTGACAAGACTGTTTTTGTTAGTACTGATTTGGATGCGCTCGCAGAACTTGCTGGTGCATCGACTGAGTTGCGCCATCGCCTCATCCCCGAACACGCACAAAAT

mRNA sequence

TTTGATCTTTTTCTCATTACCGTGTCTTTTAAGGGTGGAGATTTTGGGATTAAGCGGGCTAAAGTTGATCAAGAAACTGAACGGAAGGTTGATGGGGTATCGGATGATGTCGGAAGAATTGCCTCTGGATTAGAACCGAATTTGGCTTCTACTTCAATGGACAATGCTGCAAGCACATCGAATATTTCATCAACGGCTGGAGCAGTAAAGTCCGGCTATGATCACCTTCCAAGAGAAATGCGTGAAATGAGAATTAGAGATGACAAAACCAACCATGATGAAAAGGATTTGGTGGAACCTGCTATCATTAATGGCAGTGGAACAGAAGCTGGTCAGATAATAGCAACTACAGTAGGCGGGCGGAACGGAAAACCAAAACAGACGATCTCCTACATGGCAGAGCGTGTGGTTGGTACTGGTTCTTTCGGTGTTGTCTTCCAGGCTAAGTGCTTGGAAACAAATGAAGCTGTAGCAATAAAGAAGGTGCTGCAGGATAAGAGATACAAGAACAGGGAACTTCAGATTATGCGCATACTTGACCATCCTAATGTCGTTCAACTGAAGCATTGTTTCTTTTCCACTACTGATAAAGATGAGCTGTACCTTAACCTTGTCCTGGAGTTTATATCTGAAACTGTCTACAGAGTTTCTAAGCATTATATCAGGATGAACCGAAGCATGCCCATTATTTATGTGCAGCTTTATACATATCAGATTTGCCGGGCTTTGAATTACTTGCATCATGTTGTAGGGGTGTGTCATCGTGATATTAAGCCTCAGAATTTACTGGTTAATCCTCACACCCATCAGCTGAAGATATGTGATTTTGGGAGTGCAAAGATGTTGATGCCTGGGGAACCTAACATATCTTATATTTGTTCACGGTATTATAGGGCCCCAGAACTCATATTTGGGGCAACAGAGTATACCAATGCAATTGATATGTGGTCTGTAGGTTGTGTAATGGCTGAGCTTCTTTTAGGACAGCCTCTTTTTCCTGGTGAAAGTGGTGTTGATCAATTGGTAGAAATCATCAAGATATTGGGAACACCAACCAGAGAGGAAATTAAGTGCATGAACCCAAATTATACAGAATTCAAATTTCCTCAGATTAAAGCGCATCCATGGCATAAGATATTTCACTCGAGAATGCCTGCCGAAGCAATCGATCTTGTATCTAGATTGCTCCAGTATTCGCCCAACCTACGTTGTACTGCGTTGGAGGCTTGTGCACACCCTTTCTTTGACGATTTAAGAGATCCAAATGCATGTTTACCCAACGGCCAAGCTCTACCGCCATTGTTCAATTTCACAGCACAAGAACTTGCTGGTGCATCGACTGAGTTGCGCCATCGCCTCATCCCCGAACACGCACAAAAT

Coding sequence (CDS)

TTTGATCTTTTTCTCATTACCGTGTCTTTTAAGGGTGGAGATTTTGGGATTAAGCGGGCTAAAGTTGATCAAGAAACTGAACGGAAGGTTGATGGGGTATCGGATGATGTCGGAAGAATTGCCTCTGGATTAGAACCGAATTTGGCTTCTACTTCAATGGACAATGCTGCAAGCACATCGAATATTTCATCAACGGCTGGAGCAGTAAAGTCCGGCTATGATCACCTTCCAAGAGAAATGCGTGAAATGAGAATTAGAGATGACAAAACCAACCATGATGAAAAGGATTTGGTGGAACCTGCTATCATTAATGGCAGTGGAACAGAAGCTGGTCAGATAATAGCAACTACAGTAGGCGGGCGGAACGGAAAACCAAAACAGACGATCTCCTACATGGCAGAGCGTGTGGTTGGTACTGGTTCTTTCGGTGTTGTCTTCCAGGCTAAGTGCTTGGAAACAAATGAAGCTGTAGCAATAAAGAAGGTGCTGCAGGATAAGAGATACAAGAACAGGGAACTTCAGATTATGCGCATACTTGACCATCCTAATGTCGTTCAACTGAAGCATTGTTTCTTTTCCACTACTGATAAAGATGAGCTGTACCTTAACCTTGTCCTGGAGTTTATATCTGAAACTGTCTACAGAGTTTCTAAGCATTATATCAGGATGAACCGAAGCATGCCCATTATTTATGTGCAGCTTTATACATATCAGATTTGCCGGGCTTTGAATTACTTGCATCATGTTGTAGGGGTGTGTCATCGTGATATTAAGCCTCAGAATTTACTGGTTAATCCTCACACCCATCAGCTGAAGATATGTGATTTTGGGAGTGCAAAGATGTTGATGCCTGGGGAACCTAACATATCTTATATTTGTTCACGGTATTATAGGGCCCCAGAACTCATATTTGGGGCAACAGAGTATACCAATGCAATTGATATGTGGTCTGTAGGTTGTGTAATGGCTGAGCTTCTTTTAGGACAGCCTCTTTTTCCTGGTGAAAGTGGTGTTGATCAATTGGTAGAAATCATCAAGATATTGGGAACACCAACCAGAGAGGAAATTAAGTGCATGAACCCAAATTATACAGAATTCAAATTTCCTCAGATTAAAGCGCATCCATGGCATAAGATATTTCACTCGAGAATGCCTGCCGAAGCAATCGATCTTGTATCTAGATTGCTCCAGTATTCGCCCAACCTACGTTGTACTGCGTTGGAGGCTTGTGCACACCCTTTCTTTGACGATTTAAGAGATCCAAATGCATGTTTACCCAACGGCCAAGCTCTACCGCCATTGTTCAATTTCACAGCACAAGAACTTGCTGGTGCATCGACTGAGTTGCGCCATCGCCTCATCCCCGAACACGCACAAAAT

Protein sequence

FDLFLITVSFKGGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKSGYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCFFSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQN
Homology
BLAST of MC07g0386 vs. ExPASy Swiss-Prot
Match: Q96287 (Shaggy-related protein kinase theta OS=Arabidopsis thaliana OX=3702 GN=ASK8 PE=2 SV=3)

HSP 1 Score: 711.8 bits (1836), Expect = 4.9e-204
Identity = 355/450 (78.89%), Postives = 390/450 (86.67%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERK-VDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVK 71
           GGD+ +KRAK+DQE +   VD +   V + +S  E      S ++ A TSN+   A + K
Sbjct: 22  GGDYALKRAKLDQENDNLCVDPM--QVDQNSSCFEMKADVLSQESVAGTSNV--PAVSEK 81

Query: 72  SGYDHLPREMREMRIRDDK-TNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTI 131
              D LP  M EM+IRD++  N ++KD+ E  ++NGSGTE GQ+I TTVGGR+GKPKQTI
Sbjct: 82  PVDDQLPDVMIEMKIRDERNANREDKDM-ETTVVNGSGTETGQVITTTVGGRDGKPKQTI 141

Query: 132 SYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKH 191
           SYMA+RVVGTGSFGVVFQAKCLET E VAIKKVLQDKRYKNRELQIMR+ DHPNVV+L+H
Sbjct: 142 SYMAQRVVGTGSFGVVFQAKCLETGEQVAIKKVLQDKRYKNRELQIMRLQDHPNVVRLRH 201

Query: 192 CFFSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHV 251
            FFSTTDKDELYLNLVLE++ ETVYR SKHY +MN+ MPII+VQLYTYQICRALNYLH V
Sbjct: 202 SFFSTTDKDELYLNLVLEYVPETVYRASKHYTKMNQHMPIIFVQLYTYQICRALNYLHRV 261

Query: 252 VGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEY 311
           VGVCHRDIKPQNLLVNP THQLKICDFGSAKML+PGEPNISYICSRYYRAPELIFGATEY
Sbjct: 262 VGVCHRDIKPQNLLVNPQTHQLKICDFGSAKMLVPGEPNISYICSRYYRAPELIFGATEY 321

Query: 312 TNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFP 371
           TNAIDMWS GCVMAELLLGQPLFPGESG+DQLVEIIKILGTPTREEI+CMNPNYTEFKFP
Sbjct: 322 TNAIDMWSGGCVMAELLLGQPLFPGESGIDQLVEIIKILGTPTREEIRCMNPNYTEFKFP 381

Query: 372 QIKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQ 431
           QIKAHPWHKIFH RMP EA+DLVSRLLQYSPNLRCTALEACAHPFFDDLRDPN  LPNG+
Sbjct: 382 QIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNVSLPNGR 441

Query: 432 ALPPLFNFTAQELAGASTELRHRLIPEHAQ 460
           ALPPLFNFTAQELAGASTELR RLIP H Q
Sbjct: 442 ALPPLFNFTAQELAGASTELRQRLIPAHCQ 466

BLAST of MC07g0386 vs. ExPASy Swiss-Prot
Match: O04160 (Shaggy-related protein kinase theta OS=Brassica napus OX=3708 PE=2 SV=2)

HSP 1 Score: 695.3 bits (1793), Expect = 4.7e-199
Identity = 347/451 (76.94%), Postives = 378/451 (83.81%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           G D  +KR K+DQ+ +  +    DD  ++    +  +   S D  A TSN+         
Sbjct: 22  GVDSSLKRPKLDQDND-NLSSRGDDPMQVDQSTD--MVVVSQDTVAGTSNVPPPP----- 81

Query: 72  GYDHLPREMREMRIRDDK--TNHDEKDL-VEPAIINGSGTEAGQIIATTVGGRNGKPKQT 131
             D LP  M +MR+R+D+   N  E+D  +EP I+NG GTE GQ+I TTVGGR+GKPKQT
Sbjct: 82  --DQLPEVMNDMRLREDEPHANRGEEDKDMEPPIVNGCGTETGQVITTTVGGRDGKPKQT 141

Query: 132 ISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLK 191
           ISYMA+RVVGTGSFGVVFQAKCLET E VAIKKVLQDKRYKNRELQIMR+ DHPNVV+L+
Sbjct: 142 ISYMAQRVVGTGSFGVVFQAKCLETGEQVAIKKVLQDKRYKNRELQIMRLQDHPNVVRLR 201

Query: 192 HCFFSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHH 251
           H FFSTTDKDELYLNLVLEF+ ETVYR  KHY +MN+ MPII VQLYTYQICRALNYLH 
Sbjct: 202 HSFFSTTDKDELYLNLVLEFVPETVYRALKHYTKMNQHMPIILVQLYTYQICRALNYLHR 261

Query: 252 VVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATE 311
           VVGVCHRDIKPQNLLVN HTHQLKICDFGSAKML+PGEPNISYICSRYYRAPELIFGATE
Sbjct: 262 VVGVCHRDIKPQNLLVNTHTHQLKICDFGSAKMLVPGEPNISYICSRYYRAPELIFGATE 321

Query: 312 YTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKF 371
           YTNAIDMWS GCVMAELLLGQPLFPGESG+DQLVEIIKILGTPTREEI+CMNPNYTEFKF
Sbjct: 322 YTNAIDMWSGGCVMAELLLGQPLFPGESGIDQLVEIIKILGTPTREEIRCMNPNYTEFKF 381

Query: 372 PQIKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNG 431
           PQIKAHPWHKIFH RMP EA+DLVSRLLQYSPNLRCTALEACAHPFFDDLR PN  LPNG
Sbjct: 382 PQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEACAHPFFDDLRAPNVSLPNG 441

Query: 432 QALPPLFNFTAQELAGASTELRHRLIPEHAQ 460
           +ALPPLFNFTAQELAGASTELR RLIP H Q
Sbjct: 442 RALPPLFNFTAQELAGASTELRQRLIPAHCQ 462

BLAST of MC07g0386 vs. ExPASy Swiss-Prot
Match: Q8VZD5 (Shaggy-related protein kinase epsilon OS=Arabidopsis thaliana OX=3702 GN=ASK5 PE=1 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.6e-183
Identity = 305/386 (79.02%), Postives = 340/386 (88.08%), Query Frame = 0

Query: 74  DHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISYMA 133
           + LP  + EM+I+DDK        +E A+++G+GTE G II TT+GG+NG+PKQTISYMA
Sbjct: 24  EKLPEGINEMKIKDDKE-------MEAAVVDGNGTETGHIIVTTIGGKNGQPKQTISYMA 83

Query: 134 ERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCFFS 193
           ER+VG GSFG+VFQAKCLET E VAIKKVLQDKRYKNRELQ MR+LDHPNVV LKHCFFS
Sbjct: 84  ERIVGQGSFGIVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVSLKHCFFS 143

Query: 194 TTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVGVC 253
           TT+KDELYLNLVLE++ ETVYRVSKHY R N+ MPIIYV+LYTYQICRAL Y+H  VGVC
Sbjct: 144 TTEKDELYLNLVLEYVPETVYRVSKHYSRANQRMPIIYVKLYTYQICRALAYIHGGVGVC 203

Query: 254 HRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTNAI 313
           HRDIKPQNLLVNPHTHQ+K+CDFGSAK+L+ GEPNISYICSRYYRAPELIFGATEYT  I
Sbjct: 204 HRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTTI 263

Query: 314 DMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQIKA 373
           D+WS GCV+AELLLGQPLFPGESGVDQLVEIIK+LGTPTREEIKCMNPNYTEFKFPQIKA
Sbjct: 264 DIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEFKFPQIKA 323

Query: 374 HPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQALPP 433
           HPWHKIFH R P EA+DLVSRLLQYSPNLR TA+EA  HPFFD+LRDPN  LPNG+ALPP
Sbjct: 324 HPWHKIFHKRTPPEAVDLVSRLLQYSPNLRSTAMEAIVHPFFDELRDPNTRLPNGRALPP 383

Query: 434 LFNFTAQELAGASTELRHRLIPEHAQ 460
           LFNF  QEL GAS EL  +LIP+HA+
Sbjct: 384 LFNFKPQELKGASLELLSKLIPDHAR 402

BLAST of MC07g0386 vs. ExPASy Swiss-Prot
Match: Q40518 (Shaggy-related protein kinase NtK-1 OS=Nicotiana tabacum OX=4097 GN=NTK-1 PE=2 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 2.8e-183
Identity = 304/388 (78.35%), Postives = 340/388 (87.63%), Query Frame = 0

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           G D LP EM +MRIRDDK        +E AI++G+GTE G II TT+GGR+G+PKQTISY
Sbjct: 21  GVDRLPEEMNDMRIRDDKE-------IEAAIVDGNGTETGHIIVTTIGGRHGQPKQTISY 80

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAER+VG GSFGVVFQAKCLET E VAIKKVLQDKRYKNRELQ MR+LDHPNVV LKHCF
Sbjct: 81  MAERIVGQGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVCLKHCF 140

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTT+KDE+YLNLVLE++ ETV+RV KHY ++N+ MP+I V+LYTYQI RAL+Y+HH +G
Sbjct: 141 FSTTEKDEVYLNLVLEYVPETVHRVIKHYNKLNQRMPLILVKLYTYQIFRALSYIHHTIG 200

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQ+K+CDFGSAK+L+ GEPNISYICSRYYRAPELIFGATEYT 
Sbjct: 201 VCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTT 260

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AID+WS GCV+AELLLGQPLFPGESGVDQLVEIIK+LGTPTREEIKCMNPNY EFKFPQI
Sbjct: 261 AIDIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYNEFKFPQI 320

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFH RMP EA+DLVSRLLQYSPNLRCTALEA  H FFD+LRDPN  LPNG+ L
Sbjct: 321 KAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEAVTHAFFDELRDPNTRLPNGRVL 380

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQ 460
           PPLFNF A EL G S E   +L+PEHA+
Sbjct: 381 PPLFNFKAHELKGVSAENLLKLVPEHAR 401

BLAST of MC07g0386 vs. ExPASy Swiss-Prot
Match: P43288 (Shaggy-related protein kinase alpha OS=Arabidopsis thaliana OX=3702 GN=ASK1 PE=1 SV=3)

HSP 1 Score: 636.3 bits (1640), Expect = 2.6e-181
Identity = 300/389 (77.12%), Postives = 339/389 (87.15%), Query Frame = 0

Query: 71  SGYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTIS 130
           +G D LP EM +M+IRDDK        +E  +++G+GTE G II TT+GGRNG+PKQTIS
Sbjct: 16  TGVDKLPEEMNDMKIRDDKE-------MEATVVDGNGTETGHIIVTTIGGRNGQPKQTIS 75

Query: 131 YMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHC 190
           YMAERVVG GSFGVVFQAKCLET E VAIKKVLQD+RYKNRELQ MR+LDHPNVV LKHC
Sbjct: 76  YMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRLLDHPNVVSLKHC 135

Query: 191 FFSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVV 250
           FFSTT+KDELYLNLVLE++ ETV+RV KHY ++N+ MP+IYV+LYTYQI RAL+Y+H  +
Sbjct: 136 FFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYTYQIFRALSYIHRCI 195

Query: 251 GVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYT 310
           GVCHRDIKPQNLLVNPHTHQ+K+CDFGSAK+L+ GEPNISYICSRYYRAPELIFGATEYT
Sbjct: 196 GVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYT 255

Query: 311 NAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQ 370
            AID+WS GCV+AELLLGQPLFPGESGVDQLVEIIK+LGTPTREEIKCMNPNYTEFKFPQ
Sbjct: 256 TAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEFKFPQ 315

Query: 371 IKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQA 430
           IKAHPWHKIFH RMP EA+DLVSRLLQYSPNLR  AL+   HPFFD+LRDPNA LPNG+ 
Sbjct: 316 IKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSAALDTLVHPFFDELRDPNARLPNGRF 375

Query: 431 LPPLFNFTAQELAGASTELRHRLIPEHAQ 460
           LPPLFNF   EL G   E+  +L+PEHA+
Sbjct: 376 LPPLFNFKPHELKGVPLEMVAKLVPEHAR 397

BLAST of MC07g0386 vs. NCBI nr
Match: XP_022156124.1 (shaggy-related protein kinase theta [Momordica charantia])

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS
Sbjct: 22  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 81

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY
Sbjct: 82  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 141

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF
Sbjct: 142 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 201

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG
Sbjct: 202 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 261

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN
Sbjct: 262 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 321

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI
Sbjct: 322 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 381

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL
Sbjct: 382 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 441

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PPLFNFTAQELAGASTELRHRLIPEHAQN
Sbjct: 442 PPLFNFTAQELAGASTELRHRLIPEHAQN 470

BLAST of MC07g0386 vs. NCBI nr
Match: XP_038877594.1 (shaggy-related protein kinase theta isoform X1 [Benincasa hispida])

HSP 1 Score: 873 bits (2256), Expect = 0.0
Identity = 426/449 (94.88%), Postives = 442/449 (98.44%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           GGDFGIKRAKVDQETE+KVDGVSDD+GR+ASGLEP+LASTS+D+AASTSNISSTAG  K+
Sbjct: 22  GGDFGIKRAKVDQETEQKVDGVSDDIGRVASGLEPDLASTSLDDAASTSNISSTAGTEKT 81

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           G+DHLPREMREMRIRD+K NHDEKDL EPA++NG+GTEAGQ+IATTVGGRNGKPKQTISY
Sbjct: 82  GFDHLPREMREMRIRDEKFNHDEKDL-EPAVVNGNGTEAGQMIATTVGGRNGKPKQTISY 141

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPN+VQLKHCF
Sbjct: 142 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNIVQLKHCF 201

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTTDKDELYLNLVLE+ISETVY+VSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG
Sbjct: 202 FSTTDKDELYLNLVLEYISETVYKVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 261

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN
Sbjct: 262 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 321

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI
Sbjct: 322 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 381

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFH RMPAEAIDLVSR LQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL
Sbjct: 382 KAHPWHKIFHMRMPAEAIDLVSRFLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 441

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PPLFNFTAQELA ASTELRHRLIPEHAQN
Sbjct: 442 PPLFNFTAQELADASTELRHRLIPEHAQN 469

BLAST of MC07g0386 vs. NCBI nr
Match: XP_008460173.1 (PREDICTED: shaggy-related protein kinase theta [Cucumis melo])

HSP 1 Score: 862 bits (2226), Expect = 2.62e-314
Identity = 424/449 (94.43%), Postives = 438/449 (97.55%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           GGDFGIKRAKVD ETERKV GVS+DVGR ASGLE + ASTS+DNAASTSNISS AGA KS
Sbjct: 22  GGDFGIKRAKVDLETERKVGGVSNDVGRGASGLELDSASTSLDNAASTSNISSIAGAEKS 81

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           G+DHLPREMREMRIRD+K+NHDEKDL EPA++NG+GTEAGQIIATTVGGRNGKPKQTISY
Sbjct: 82  GFDHLPREMREMRIRDEKSNHDEKDL-EPAVVNGNGTEAGQIIATTVGGRNGKPKQTISY 141

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMR+LDH N+VQLKHCF
Sbjct: 142 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRMLDHSNIVQLKHCF 201

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTTDKDELYLNLVLE+ISETVY+VSKHYIRMNRSMPI+YVQLYTYQICRALNYLHHVVG
Sbjct: 202 FSTTDKDELYLNLVLEYISETVYKVSKHYIRMNRSMPIVYVQLYTYQICRALNYLHHVVG 261

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN
Sbjct: 262 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 321

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI
Sbjct: 322 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 381

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFH RMP EAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL
Sbjct: 382 KAHPWHKIFHMRMPVEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 441

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PPLFNFTAQELAGASTELRHRLIPEHAQN
Sbjct: 442 PPLFNFTAQELAGASTELRHRLIPEHAQN 469

BLAST of MC07g0386 vs. NCBI nr
Match: TYK24466.1 (shaggy-related protein kinase theta [Cucumis melo var. makuwa])

HSP 1 Score: 862 bits (2228), Expect = 2.67e-314
Identity = 428/460 (93.04%), Postives = 443/460 (96.30%), Query Frame = 0

Query: 1   FDLFLITVSFKGGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTS 60
           F+L  I V   GGDFGIKRAKVD ETERKV GVS+DVGR ASGLE + ASTS+DNAASTS
Sbjct: 32  FELMDIVV-LPGGDFGIKRAKVDLETERKVGGVSNDVGRGASGLELDSASTSLDNAASTS 91

Query: 61  NISSTAGAVKSGYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGG 120
           NISS AGA KSG+DHLPREMREMRIRD+K+NHDEKDL EPA++NG+GTEAGQIIATTVGG
Sbjct: 92  NISSIAGAEKSGFDHLPREMREMRIRDEKSNHDEKDL-EPAVVNGNGTEAGQIIATTVGG 151

Query: 121 RNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILD 180
           RNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMR+LD
Sbjct: 152 RNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRMLD 211

Query: 181 HPNVVQLKHCFFSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQIC 240
           H N+VQLKHCFFSTTDKDELYLNLVLE+ISETVY+VSKHYIRMNRSMPI+YVQLYTYQIC
Sbjct: 212 HSNIVQLKHCFFSTTDKDELYLNLVLEYISETVYKVSKHYIRMNRSMPIVYVQLYTYQIC 271

Query: 241 RALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAP 300
           RALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAP
Sbjct: 272 RALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAP 331

Query: 301 ELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMN 360
           ELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMN
Sbjct: 332 ELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMN 391

Query: 361 PNYTEFKFPQIKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRD 420
           PNYTEFKFPQIKAHPWHKIFH RMP EAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRD
Sbjct: 392 PNYTEFKFPQIKAHPWHKIFHMRMPVEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRD 451

Query: 421 PNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQN
Sbjct: 452 PNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQN 489

BLAST of MC07g0386 vs. NCBI nr
Match: XP_004144981.1 (shaggy-related protein kinase theta isoform X1 [Cucumis sativus] >KGN46198.1 hypothetical protein Csa_005607 [Cucumis sativus])

HSP 1 Score: 855 bits (2209), Expect = 9.99e-312
Identity = 420/449 (93.54%), Postives = 436/449 (97.10%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           GGDFGIKRAKVDQETE+KV GVSDDVGR ASGL  + +S+S+DN ASTSNISS AGA KS
Sbjct: 22  GGDFGIKRAKVDQETEQKVGGVSDDVGRAASGLGLDSSSSSLDNVASTSNISSIAGAEKS 81

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           G+DHLPREMREMRI+D+K+NHDEKDL EPA++NG+GTEAGQIIATTVGGRNGKPKQTISY
Sbjct: 82  GFDHLPREMREMRIKDEKSNHDEKDL-EPAVVNGNGTEAGQIIATTVGGRNGKPKQTISY 141

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMR+LDH N+VQLKHCF
Sbjct: 142 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRMLDHSNIVQLKHCF 201

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTTDKDELYLNLVLE+ISETVY+VSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG
Sbjct: 202 FSTTDKDELYLNLVLEYISETVYKVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 261

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN
Sbjct: 262 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 321

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI
Sbjct: 322 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 381

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFH RMP EAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL
Sbjct: 382 KAHPWHKIFHMRMPVEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 441

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PPLFNFTAQELAGASTELR RLIPEHAQN
Sbjct: 442 PPLFNFTAQELAGASTELRQRLIPEHAQN 469

BLAST of MC07g0386 vs. ExPASy TrEMBL
Match: A0A6J1DSE6 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111023089 PE=3 SV=1)

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 449/449 (100.00%), Postives = 449/449 (100.00%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS
Sbjct: 22  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 81

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY
Sbjct: 82  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 141

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF
Sbjct: 142 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 201

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG
Sbjct: 202 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 261

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN
Sbjct: 262 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 321

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI
Sbjct: 322 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 381

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL
Sbjct: 382 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 441

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PPLFNFTAQELAGASTELRHRLIPEHAQN
Sbjct: 442 PPLFNFTAQELAGASTELRHRLIPEHAQN 470

BLAST of MC07g0386 vs. ExPASy TrEMBL
Match: A0A1S3CC12 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103499062 PE=3 SV=1)

HSP 1 Score: 862 bits (2226), Expect = 1.27e-314
Identity = 424/449 (94.43%), Postives = 438/449 (97.55%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           GGDFGIKRAKVD ETERKV GVS+DVGR ASGLE + ASTS+DNAASTSNISS AGA KS
Sbjct: 22  GGDFGIKRAKVDLETERKVGGVSNDVGRGASGLELDSASTSLDNAASTSNISSIAGAEKS 81

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           G+DHLPREMREMRIRD+K+NHDEKDL EPA++NG+GTEAGQIIATTVGGRNGKPKQTISY
Sbjct: 82  GFDHLPREMREMRIRDEKSNHDEKDL-EPAVVNGNGTEAGQIIATTVGGRNGKPKQTISY 141

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMR+LDH N+VQLKHCF
Sbjct: 142 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRMLDHSNIVQLKHCF 201

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTTDKDELYLNLVLE+ISETVY+VSKHYIRMNRSMPI+YVQLYTYQICRALNYLHHVVG
Sbjct: 202 FSTTDKDELYLNLVLEYISETVYKVSKHYIRMNRSMPIVYVQLYTYQICRALNYLHHVVG 261

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN
Sbjct: 262 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 321

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI
Sbjct: 322 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 381

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFH RMP EAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL
Sbjct: 382 KAHPWHKIFHMRMPVEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 441

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PPLFNFTAQELAGASTELRHRLIPEHAQN
Sbjct: 442 PPLFNFTAQELAGASTELRHRLIPEHAQN 469

BLAST of MC07g0386 vs. ExPASy TrEMBL
Match: A0A5D3DLF3 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00070 PE=3 SV=1)

HSP 1 Score: 862 bits (2228), Expect = 1.29e-314
Identity = 428/460 (93.04%), Postives = 443/460 (96.30%), Query Frame = 0

Query: 1   FDLFLITVSFKGGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTS 60
           F+L  I V   GGDFGIKRAKVD ETERKV GVS+DVGR ASGLE + ASTS+DNAASTS
Sbjct: 32  FELMDIVV-LPGGDFGIKRAKVDLETERKVGGVSNDVGRGASGLELDSASTSLDNAASTS 91

Query: 61  NISSTAGAVKSGYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGG 120
           NISS AGA KSG+DHLPREMREMRIRD+K+NHDEKDL EPA++NG+GTEAGQIIATTVGG
Sbjct: 92  NISSIAGAEKSGFDHLPREMREMRIRDEKSNHDEKDL-EPAVVNGNGTEAGQIIATTVGG 151

Query: 121 RNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILD 180
           RNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMR+LD
Sbjct: 152 RNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRMLD 211

Query: 181 HPNVVQLKHCFFSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQIC 240
           H N+VQLKHCFFSTTDKDELYLNLVLE+ISETVY+VSKHYIRMNRSMPI+YVQLYTYQIC
Sbjct: 212 HSNIVQLKHCFFSTTDKDELYLNLVLEYISETVYKVSKHYIRMNRSMPIVYVQLYTYQIC 271

Query: 241 RALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAP 300
           RALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAP
Sbjct: 272 RALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAP 331

Query: 301 ELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMN 360
           ELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMN
Sbjct: 332 ELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMN 391

Query: 361 PNYTEFKFPQIKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRD 420
           PNYTEFKFPQIKAHPWHKIFH RMP EAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRD
Sbjct: 392 PNYTEFKFPQIKAHPWHKIFHMRMPVEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRD 451

Query: 421 PNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQN
Sbjct: 452 PNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQN 489

BLAST of MC07g0386 vs. ExPASy TrEMBL
Match: A0A0A0K9N3 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6G074020 PE=3 SV=1)

HSP 1 Score: 855 bits (2209), Expect = 4.84e-312
Identity = 420/449 (93.54%), Postives = 436/449 (97.10%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           GGDFGIKRAKVDQETE+KV GVSDDVGR ASGL  + +S+S+DN ASTSNISS AGA KS
Sbjct: 22  GGDFGIKRAKVDQETEQKVGGVSDDVGRAASGLGLDSSSSSLDNVASTSNISSIAGAEKS 81

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           G+DHLPREMREMRI+D+K+NHDEKDL EPA++NG+GTEAGQIIATTVGGRNGKPKQTISY
Sbjct: 82  GFDHLPREMREMRIKDEKSNHDEKDL-EPAVVNGNGTEAGQIIATTVGGRNGKPKQTISY 141

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMR+LDH N+VQLKHCF
Sbjct: 142 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRMLDHSNIVQLKHCF 201

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTTDKDELYLNLVLE+ISETVY+VSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG
Sbjct: 202 FSTTDKDELYLNLVLEYISETVYKVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 261

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN
Sbjct: 262 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 321

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI
Sbjct: 322 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 381

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFH RMP EAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL
Sbjct: 382 KAHPWHKIFHMRMPVEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 441

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PPLFNFTAQELAGASTELR RLIPEHAQN
Sbjct: 442 PPLFNFTAQELAGASTELRQRLIPEHAQN 469

BLAST of MC07g0386 vs. ExPASy TrEMBL
Match: A0A6J1L4E3 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111499041 PE=3 SV=1)

HSP 1 Score: 852 bits (2202), Expect = 5.64e-311
Identity = 417/449 (92.87%), Postives = 437/449 (97.33%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERKVDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVKS 71
           GGDFGIKRAKVDQETE+KVDGVSDDV R+ASGL+P+LA TS+DNAASTSNISSTAGA KS
Sbjct: 22  GGDFGIKRAKVDQETEQKVDGVSDDVERVASGLDPDLACTSLDNAASTSNISSTAGAEKS 81

Query: 72  GYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISY 131
           G+DHLPREMREMRIRD+K+NHDEKD  EP ++NG+GTEAGQIIATTVGGRNG PKQTISY
Sbjct: 82  GFDHLPREMREMRIRDEKSNHDEKDF-EPDVVNGNGTEAGQIIATTVGGRNGIPKQTISY 141

Query: 132 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCF 191
           MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQ MRIL+HPN+VQLKHCF
Sbjct: 142 MAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQTMRILNHPNIVQLKHCF 201

Query: 192 FSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVG 251
           FSTTDKDELYLNLVLE+IS+TVY+VSKHYIR +RSMPIIYVQLYTYQICRALNYLH VVG
Sbjct: 202 FSTTDKDELYLNLVLEYISKTVYKVSKHYIRTHRSMPIIYVQLYTYQICRALNYLHQVVG 261

Query: 252 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 311
           VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN
Sbjct: 262 VCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTN 321

Query: 312 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 371
           AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI
Sbjct: 322 AIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQI 381

Query: 372 KAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 431
           KAHPWHKIFH RMPA+AIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL
Sbjct: 382 KAHPWHKIFHMRMPADAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQAL 441

Query: 432 PPLFNFTAQELAGASTELRHRLIPEHAQN 460
           PPLFNFTAQELAGASTELRH LIPEHA++
Sbjct: 442 PPLFNFTAQELAGASTELRHHLIPEHARS 469

BLAST of MC07g0386 vs. TAIR 10
Match: AT4G00720.1 (shaggy-like protein kinase 32 )

HSP 1 Score: 711.8 bits (1836), Expect = 3.5e-205
Identity = 355/450 (78.89%), Postives = 390/450 (86.67%), Query Frame = 0

Query: 12  GGDFGIKRAKVDQETERK-VDGVSDDVGRIASGLEPNLASTSMDNAASTSNISSTAGAVK 71
           GGD+ +KRAK+DQE +   VD +   V + +S  E      S ++ A TSN+   A + K
Sbjct: 22  GGDYALKRAKLDQENDNLCVDPM--QVDQNSSCFEMKADVLSQESVAGTSNV--PAVSEK 81

Query: 72  SGYDHLPREMREMRIRDDK-TNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTI 131
              D LP  M EM+IRD++  N ++KD+ E  ++NGSGTE GQ+I TTVGGR+GKPKQTI
Sbjct: 82  PVDDQLPDVMIEMKIRDERNANREDKDM-ETTVVNGSGTETGQVITTTVGGRDGKPKQTI 141

Query: 132 SYMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKH 191
           SYMA+RVVGTGSFGVVFQAKCLET E VAIKKVLQDKRYKNRELQIMR+ DHPNVV+L+H
Sbjct: 142 SYMAQRVVGTGSFGVVFQAKCLETGEQVAIKKVLQDKRYKNRELQIMRLQDHPNVVRLRH 201

Query: 192 CFFSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHV 251
            FFSTTDKDELYLNLVLE++ ETVYR SKHY +MN+ MPII+VQLYTYQICRALNYLH V
Sbjct: 202 SFFSTTDKDELYLNLVLEYVPETVYRASKHYTKMNQHMPIIFVQLYTYQICRALNYLHRV 261

Query: 252 VGVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEY 311
           VGVCHRDIKPQNLLVNP THQLKICDFGSAKML+PGEPNISYICSRYYRAPELIFGATEY
Sbjct: 262 VGVCHRDIKPQNLLVNPQTHQLKICDFGSAKMLVPGEPNISYICSRYYRAPELIFGATEY 321

Query: 312 TNAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFP 371
           TNAIDMWS GCVMAELLLGQPLFPGESG+DQLVEIIKILGTPTREEI+CMNPNYTEFKFP
Sbjct: 322 TNAIDMWSGGCVMAELLLGQPLFPGESGIDQLVEIIKILGTPTREEIRCMNPNYTEFKFP 381

Query: 372 QIKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQ 431
           QIKAHPWHKIFH RMP EA+DLVSRLLQYSPNLRCTALEACAHPFFDDLRDPN  LPNG+
Sbjct: 382 QIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNVSLPNGR 441

Query: 432 ALPPLFNFTAQELAGASTELRHRLIPEHAQ 460
           ALPPLFNFTAQELAGASTELR RLIP H Q
Sbjct: 442 ALPPLFNFTAQELAGASTELRQRLIPAHCQ 466

BLAST of MC07g0386 vs. TAIR 10
Match: AT5G14640.1 (shaggy-like kinase 13 )

HSP 1 Score: 643.7 bits (1659), Expect = 1.2e-184
Identity = 305/386 (79.02%), Postives = 340/386 (88.08%), Query Frame = 0

Query: 74  DHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTISYMA 133
           + LP  + EM+I+DDK        +E A+++G+GTE G II TT+GG+NG+PKQTISYMA
Sbjct: 24  EKLPEGINEMKIKDDKE-------MEAAVVDGNGTETGHIIVTTIGGKNGQPKQTISYMA 83

Query: 134 ERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHCFFS 193
           ER+VG GSFG+VFQAKCLET E VAIKKVLQDKRYKNRELQ MR+LDHPNVV LKHCFFS
Sbjct: 84  ERIVGQGSFGIVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLLDHPNVVSLKHCFFS 143

Query: 194 TTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVVGVC 253
           TT+KDELYLNLVLE++ ETVYRVSKHY R N+ MPIIYV+LYTYQICRAL Y+H  VGVC
Sbjct: 144 TTEKDELYLNLVLEYVPETVYRVSKHYSRANQRMPIIYVKLYTYQICRALAYIHGGVGVC 203

Query: 254 HRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYTNAI 313
           HRDIKPQNLLVNPHTHQ+K+CDFGSAK+L+ GEPNISYICSRYYRAPELIFGATEYT  I
Sbjct: 204 HRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTTI 263

Query: 314 DMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQIKA 373
           D+WS GCV+AELLLGQPLFPGESGVDQLVEIIK+LGTPTREEIKCMNPNYTEFKFPQIKA
Sbjct: 264 DIWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEFKFPQIKA 323

Query: 374 HPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQALPP 433
           HPWHKIFH R P EA+DLVSRLLQYSPNLR TA+EA  HPFFD+LRDPN  LPNG+ALPP
Sbjct: 324 HPWHKIFHKRTPPEAVDLVSRLLQYSPNLRSTAMEAIVHPFFDELRDPNTRLPNGRALPP 383

Query: 434 LFNFTAQELAGASTELRHRLIPEHAQ 460
           LFNF  QEL GAS EL  +LIP+HA+
Sbjct: 384 LFNFKPQELKGASLELLSKLIPDHAR 402

BLAST of MC07g0386 vs. TAIR 10
Match: AT5G26751.1 (shaggy-related kinase 11 )

HSP 1 Score: 636.3 bits (1640), Expect = 1.9e-182
Identity = 300/389 (77.12%), Postives = 339/389 (87.15%), Query Frame = 0

Query: 71  SGYDHLPREMREMRIRDDKTNHDEKDLVEPAIINGSGTEAGQIIATTVGGRNGKPKQTIS 130
           +G D LP EM +M+IRDDK        +E  +++G+GTE G II TT+GGRNG+PKQTIS
Sbjct: 16  TGVDKLPEEMNDMKIRDDKE-------MEATVVDGNGTETGHIIVTTIGGRNGQPKQTIS 75

Query: 131 YMAERVVGTGSFGVVFQAKCLETNEAVAIKKVLQDKRYKNRELQIMRILDHPNVVQLKHC 190
           YMAERVVG GSFGVVFQAKCLET E VAIKKVLQD+RYKNRELQ MR+LDHPNVV LKHC
Sbjct: 76  YMAERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRLLDHPNVVSLKHC 135

Query: 191 FFSTTDKDELYLNLVLEFISETVYRVSKHYIRMNRSMPIIYVQLYTYQICRALNYLHHVV 250
           FFSTT+KDELYLNLVLE++ ETV+RV KHY ++N+ MP+IYV+LYTYQI RAL+Y+H  +
Sbjct: 136 FFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYTYQIFRALSYIHRCI 195

Query: 251 GVCHRDIKPQNLLVNPHTHQLKICDFGSAKMLMPGEPNISYICSRYYRAPELIFGATEYT 310
           GVCHRDIKPQNLLVNPHTHQ+K+CDFGSAK+L+ GEPNISYICSRYYRAPELIFGATEYT
Sbjct: 196 GVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYT 255

Query: 311 NAIDMWSVGCVMAELLLGQPLFPGESGVDQLVEIIKILGTPTREEIKCMNPNYTEFKFPQ 370
            AID+WS GCV+AELLLGQPLFPGESGVDQLVEIIK+LGTPTREEIKCMNPNYTEFKFPQ
Sbjct: 256 TAIDVWSAGCVLAELLLGQPLFPGESGVDQLVEIIKVLGTPTREEIKCMNPNYTEFKFPQ 315

Query: 371 IKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNLRCTALEACAHPFFDDLRDPNACLPNGQA 430
           IKAHPWHKIFH RMP EA+DLVSRLLQYSPNLR  AL+   HPFFD+LRDPNA LPNG+ 
Sbjct: 316 IKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSAALDTLVHPFFDELRDPNARLPNGRF 375

Query: 431 LPPLFNFTAQELAGASTELRHRLIPEHAQ 460
           LPPLFNF   EL G   E+  +L+PEHA+
Sbjct: 376 LPPLFNFKPHELKGVPLEMVAKLVPEHAR 397

BLAST of MC07g0386 vs. TAIR 10
Match: AT3G05840.2 (Protein kinase superfamily protein )

HSP 1 Score: 635.6 bits (1638), Expect = 3.2e-182
Identity = 305/417 (73.14%), Postives = 350/417 (83.93%), Query Frame = 0

Query: 43  GLEPNLASTSMDNAASTSNISSTAGAVKSGYDHLPREMREMRIRDDKTNHDEKDLVEPAI 102
           G+EP+ A        ST N++          D LP EM++M+I+DDK        +E  I
Sbjct: 5   GIEPSAAVRE-----STGNVTDA--------DRLPEEMKDMKIQDDKE-------MEATI 64

Query: 103 INGSGTEAGQIIATTVGGRNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKV 162
           +NG+ TE G II TT+GGRNG+PKQTISYMAERVVG GSFGVVFQAKCLET E VAIKKV
Sbjct: 65  VNGNVTETGHIIVTTIGGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKV 124

Query: 163 LQDKRYKNRELQIMRILDHPNVVQLKHCFFSTTDKDELYLNLVLEFISETVYRVSKHYIR 222
           LQD+RYKNRELQ MR+LDHPNVV LKHCFFSTT+KDELYLNLVLE++ ETV+RV KHY +
Sbjct: 125 LQDRRYKNRELQTMRLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK 184

Query: 223 MNRSMPIIYVQLYTYQICRALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKML 282
           +N+ MP++YV+LYTYQI R+L+Y+H  +GVCHRDIKPQNLLVNPHTHQ+K+CDFGSAK+L
Sbjct: 185 LNQRMPLVYVKLYTYQIFRSLSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVL 244

Query: 283 MPGEPNISYICSRYYRAPELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLV 342
           + GEPNISYICSRYYRAPELIFGATEYT AID+WS GCV+AELLLGQPLFPGESGVDQLV
Sbjct: 245 VKGEPNISYICSRYYRAPELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLV 304

Query: 343 EIIKILGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNL 402
           EIIK+LGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFH RMP EA+DLVSRLLQYSPNL
Sbjct: 305 EIIKVLGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNL 364

Query: 403 RCTALEACAHPFFDDLRDPNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQ 460
           RC AL++  HPFFD+LRDPNA LPNG+ LPPLFNF   EL G   E+  +L+PEHA+
Sbjct: 365 RCAALDSLVHPFFDELRDPNARLPNGRFLPPLFNFKPHELKGVPVEMVAKLVPEHAR 401

BLAST of MC07g0386 vs. TAIR 10
Match: AT3G05840.1 (Protein kinase superfamily protein )

HSP 1 Score: 635.6 bits (1638), Expect = 3.2e-182
Identity = 305/417 (73.14%), Postives = 350/417 (83.93%), Query Frame = 0

Query: 43  GLEPNLASTSMDNAASTSNISSTAGAVKSGYDHLPREMREMRIRDDKTNHDEKDLVEPAI 102
           G+EP+ A        ST N++          D LP EM++M+I+DDK        +E  I
Sbjct: 5   GIEPSAAVRE-----STGNVTDA--------DRLPEEMKDMKIQDDKE-------MEATI 64

Query: 103 INGSGTEAGQIIATTVGGRNGKPKQTISYMAERVVGTGSFGVVFQAKCLETNEAVAIKKV 162
           +NG+ TE G II TT+GGRNG+PKQTISYMAERVVG GSFGVVFQAKCLET E VAIKKV
Sbjct: 65  VNGNVTETGHIIVTTIGGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGETVAIKKV 124

Query: 163 LQDKRYKNRELQIMRILDHPNVVQLKHCFFSTTDKDELYLNLVLEFISETVYRVSKHYIR 222
           LQD+RYKNRELQ MR+LDHPNVV LKHCFFSTT+KDELYLNLVLE++ ETV+RV KHY +
Sbjct: 125 LQDRRYKNRELQTMRLLDHPNVVSLKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNK 184

Query: 223 MNRSMPIIYVQLYTYQICRALNYLHHVVGVCHRDIKPQNLLVNPHTHQLKICDFGSAKML 282
           +N+ MP++YV+LYTYQI R+L+Y+H  +GVCHRDIKPQNLLVNPHTHQ+K+CDFGSAK+L
Sbjct: 185 LNQRMPLVYVKLYTYQIFRSLSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVL 244

Query: 283 MPGEPNISYICSRYYRAPELIFGATEYTNAIDMWSVGCVMAELLLGQPLFPGESGVDQLV 342
           + GEPNISYICSRYYRAPELIFGATEYT AID+WS GCV+AELLLGQPLFPGESGVDQLV
Sbjct: 245 VKGEPNISYICSRYYRAPELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLV 304

Query: 343 EIIKILGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFHSRMPAEAIDLVSRLLQYSPNL 402
           EIIK+LGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFH RMP EA+DLVSRLLQYSPNL
Sbjct: 305 EIIKVLGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNL 364

Query: 403 RCTALEACAHPFFDDLRDPNACLPNGQALPPLFNFTAQELAGASTELRHRLIPEHAQ 460
           RC AL++  HPFFD+LRDPNA LPNG+ LPPLFNF   EL G   E+  +L+PEHA+
Sbjct: 365 RCAALDSLVHPFFDELRDPNARLPNGRFLPPLFNFKPHELKGVPVEMVAKLVPEHAR 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q962874.9e-20478.89Shaggy-related protein kinase theta OS=Arabidopsis thaliana OX=3702 GN=ASK8 PE=2... [more]
O041604.7e-19976.94Shaggy-related protein kinase theta OS=Brassica napus OX=3708 PE=2 SV=2[more]
Q8VZD51.6e-18379.02Shaggy-related protein kinase epsilon OS=Arabidopsis thaliana OX=3702 GN=ASK5 PE... [more]
Q405182.8e-18378.35Shaggy-related protein kinase NtK-1 OS=Nicotiana tabacum OX=4097 GN=NTK-1 PE=2 S... [more]
P432882.6e-18177.12Shaggy-related protein kinase alpha OS=Arabidopsis thaliana OX=3702 GN=ASK1 PE=1... [more]
Match NameE-valueIdentityDescription
XP_022156124.10.0100.00shaggy-related protein kinase theta [Momordica charantia][more]
XP_038877594.10.094.88shaggy-related protein kinase theta isoform X1 [Benincasa hispida][more]
XP_008460173.12.62e-31494.43PREDICTED: shaggy-related protein kinase theta [Cucumis melo][more]
TYK24466.12.67e-31493.04shaggy-related protein kinase theta [Cucumis melo var. makuwa][more]
XP_004144981.19.99e-31293.54shaggy-related protein kinase theta isoform X1 [Cucumis sativus] >KGN46198.1 hyp... [more]
Match NameE-valueIdentityDescription
A0A6J1DSE60.0100.00Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A1S3CC121.27e-31494.43Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5D3DLF31.29e-31493.04Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0K9N34.84e-31293.54Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6... [more]
A0A6J1L4E35.64e-31192.87Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G00720.13.5e-20578.89shaggy-like protein kinase 32 [more]
AT5G14640.11.2e-18479.02shaggy-like kinase 13 [more]
AT5G26751.11.9e-18277.12shaggy-related kinase 11 [more]
AT3G05840.23.2e-18273.14Protein kinase superfamily protein [more]
AT3G05840.13.2e-18273.14Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 131..415
e-value: 8.8E-88
score: 307.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 131..415
e-value: 1.4E-64
score: 218.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 131..415
score: 43.509411
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 211..459
e-value: 2.3E-82
score: 278.1
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 116..210
e-value: 2.9E-34
score: 118.5
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 235..379
e-value: 9.1E-12
score: 42.3
NoneNo IPR availablePANTHERPTHR24057:SF43SHAGGY-LIKE KINASEcoord: 44..459
NoneNo IPR availablePANTHERPTHR24057GLYCOGEN SYNTHASE KINASE-3 ALPHAcoord: 44..459
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 252..264
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 137..161
IPR039192Glycogen synthase kinase 3, catalytic domainCDDcd14137STKc_GSK3coord: 126..418
e-value: 0.0
score: 597.562
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 130..441

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0386.1MC07g0386.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity