MC07g0190 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0190
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	rRNA N-glycosidase
Location	MC07: 4371352 .. 4372115 (+)
RNA-Seq Expression	MC07g0190
Synteny	MC07g0190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGTAGCCTTTTACTTTGTTCTCGCTCTCTATCTTGGCGCTCGAGCTACAGAGGCTGATGTATCATTTTCTTTACTAGGTGCCACCTCCAAAACCTACAAACAGTTCATAACCAATCTACGTAGTGCCCTCACGGTGAGATCTCCAATGGTGTACGATATCCTTGTGTTGTACCCGCCAACAACGGCCTCGACGCGCTTCATATTACTGCATCTCACAAATTACAATACTGAATCCATCACTGTGGCAATAAACATAGTGAACGTGTACGTTGTAGCATATCGAGCTGGAGATAATGCATACTTTCTTAACGATTCCTCAGCAGAAGCAGACAATGTTCTACTCAAAGGCATCAAACATGAAAGACTTTCCTATAAAGGAAACTAGACTTTCCTATAAAGGAAACTATGACGGCTTTGAGAGGGCTGCAGGGAAGATTTCGAGACAGAACATTAATCTCGGGTTTGTCTGAAATGAGTAGTGCCATTGGCAGCATGTTCCACCACAATGTTGGCACTTCTGTTCCTAGAGCATTTATTGTGATCATCCAAATAGTCTTAGAAGCTGTAAGATTTAAGTATACTGAGCACGGAGTTTCTGAGCATGTCAAGACCAGCTTTAAGCGAGACCCTACGTTCTTCAACTTAGAAAATCGTTGGACTGATTTCTCTGAACATATACAAATTGCACAGATACGAGATGGGGAATTTGGTCGTCCTGTTGAGCTTAGAACTATTTACAACAAACCAATTCTCGTAACTAAT

mRNA sequence

TTGGTAGCCTTTTACTTTGTTCTCGCTCTCTATCTTGGCGCTCGAGCTACAGAGGCTGATGTATCATTTTCTTTACTAGGTGCCACCTCCAAAACCTACAAACAGTTCATAACCAATCTACGTAGTGCCCTCACGGTGAGATCTCCAATGGTGTACGATATCCTTGTGTTGTACCCGCCAACAACGGCCTCGACGCGCTTCATATTACTGCATCTCACAAATTACAATACTGAATCCATCACTGTGGCAATAAACATAGTGAACGTGTACGTTGTAGCATATCGAGCTGGAGATAATGCATACTTTCTTAACGATTCCTCAGCAGAAGCAGACAATGTTCTACTCAAAGGCATCAAACATGAAAGACTTTCCTATAAAGGAAACGACTTTCCTATAAAGGAAACTATGACGGCTTTGAGAGGGCTGCAGGGAAGATTTCGAGACAGAACATTAATCTCGGGTTTGTCTGAAATGAGTAGTGCCATTGGCAGCATGTTCCACCACAATGTTGGCACTTCTGTTCCTAGAGCATTTATTGTGATCATCCAAATAGTCTTAGAAGCTGTAAGATTTAAGTATACTGAGCACGGAGTTTCTGAGCATGTCAAGACCAGCTTTAAGCGAGACCCTACGTTCTTCAACTTAGAAAATCGTTGGACTGATTTCTCTGAACATATACAAATTGCACAGATACGAGATGGGGAATTTGGTCGTCCTGTTGAGCTTAGAACTATTTACAACAAACCAATTCTCGTAACTAAT

Coding sequence (CDS)

TTGGTAGCCTTTTACTTTGTTCTCGCTCTCTATCTTGGCGCTCGAGCTACAGAGGCTGATGTATCATTTTCTTTACTAGGTGCCACCTCCAAAACCTACAAACAGTTCATAACCAATCTACGTAGTGCCCTCACGGTGAGATCTCCAATGGTGTACGATATCCTTGTGTTGTACCCGCCAACAACGGCCTCGACGCGCTTCATATTACTGCATCTCACAAATTACAATACTGAATCCATCACTGTGGCAATAAACATAGTGAACGTGTACGTTGTAGCATATCGAGCTGGAGATAATGCATACTTTCTTAACGATTCCTCAGCAGAAGCAGACAATGTTCTACTCAAAGGCATCAAACATGAAAGACTTTCCTATAAAGGAAACGACTTTCCTATAAAGGAAACTATGACGGCTTTGAGAGGGCTGCAGGGAAGATTTCGAGACAGAACATTAATCTCGGGTTTGTCTGAAATGAGTAGTGCCATTGGCAGCATGTTCCACCACAATGTTGGCACTTCTGTTCCTAGAGCATTTATTGTGATCATCCAAATAGTCTTAGAAGCTGTAAGATTTAAGTATACTGAGCACGGAGTTTCTGAGCATGTCAAGACCAGCTTTAAGCGAGACCCTACGTTCTTCAACTTAGAAAATCGTTGGACTGATTTCTCTGAACATATACAAATTGCACAGATACGAGATGGGGAATTTGGTCGTCCTGTTGAGCTTAGAACTATTTACAACAAACCAATTCTCGTAACTAAT

Protein sequence

LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPPTTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKHERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIVIIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPVELRTIYNKPILVTN

Homology

[Close]

BLAST of MC07g0190 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-71
Identity = 151/254 (59.45%), Postives = 173/254 (68.11%), Query Frame = 0


Query: 2   VAFYFVLALYLGARATE-ADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 61
           + FY VLALY+GA  TE  D++FSL+GAT  TYK FI NLR+ LTV +P VYDI VL   
Sbjct: 4   IGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNA 63

Query: 62  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKH 121
                RF L+ LTNYN ES+TVA+++VNVYVVAYRAG+ AYFL D+S EA+NVL  GI H
Sbjct: 64  AAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINH 123

Query: 122 ERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIV 181
            RL Y GN          L    GR     +  G SE+SSAIG+MF HN GTSVPRAFIV
Sbjct: 124 VRLPYGGN-------YDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIV 183

Query: 182 IIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPV 241
           IIQ V EA RFKY E  VSE+V T FK DP F +L+N W   SE IQIAQ R GEF RPV
Sbjct: 184 IIQTVSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPV 243

Query: 242 ELRTIYNKPILVTN 255
           ELRT+ N P  VTN
Sbjct: 244 ELRTVSNTPTFVTN 250

[Close]

BLAST of MC07g0190 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 168.7 bits (426), Expect = 8.5e-41
Identity = 103/254 (40.55%), Postives = 153/254 (60.24%), Query Frame = 0


Query: 1   LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 60
           L+  + ++A+++G    + DV+F L  AT+KTY +FI + R+ L   S  VYDI +LY  
Sbjct: 5   LLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYST 64

Query: 61  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKH 120
            + S RFILL+LT+Y  E+I+VAI++ NVYVVAYR  D +YF  +S  EA N+L KG + 
Sbjct: 65  ISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRK 124

Query: 121 ERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIV 180
             L Y GN    +   TA   ++       +  GL  +SSAI ++F++N   S P A +V
Sbjct: 125 ITLPYTGN---YENLQTAAHKIR-----ENIDLGLPALSSAITTLFYYN-AQSAPSALLV 184

Query: 181 IIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPV 240
           +IQ   EA RFKY E  V+++V T+FK +    +LEN+W+  S+ I +AQ + G+F  PV
Sbjct: 185 LIQTTAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPV 244

Query: 241 ELRTIYNKPILVTN 255
           +L     +   VTN
Sbjct: 245 DLIKPTGERFQVTN 248

[Close]

BLAST of MC07g0190 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.4e-40
Identity = 103/254 (40.55%), Postives = 152/254 (59.84%), Query Frame = 0


Query: 1   LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 60
           L+  + ++A+++G    + DV+F L  AT+KTY +FI + R+ L   S  VYDI +LY  
Sbjct: 5   LLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYST 64

Query: 61  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKH 120
            + S RFILL LT+Y  E+I+VAI++ NVYVVAYR  D +YF  +S  EA N+L KG + 
Sbjct: 65  ISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRK 124

Query: 121 ERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIV 180
             L Y GN    +   TA   ++       +  GL  +SSAI ++F++N   S P A +V
Sbjct: 125 ITLPYTGN---YENLQTAAHKIR-----ENIDLGLPALSSAITTLFYYN-AQSAPSALLV 184

Query: 181 IIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPV 240
           +IQ   EA RFKY E  V+++V T+FK +    +LEN+W+  S+ I +AQ + G+F  PV
Sbjct: 185 LIQTTAEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQGGKFRNPV 244

Query: 241 ELRTIYNKPILVTN 255
           +L     +   VTN
Sbjct: 245 DLIKPTGERFQVTN 248

[Close]

BLAST of MC07g0190 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 158.7 bits (400), Expect = 8.8e-38
Identity = 103/256 (40.23%), Postives = 145/256 (56.64%), Query Frame = 0


Query: 1   LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 60
           LV    +L L+L A A E DVSF L GATS +Y  FI+NLR AL     + YDI +L   
Sbjct: 5   LVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYERKL-YDIPLLRST 64

Query: 61  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSA-EADNVLLKGIK 120
              S R+ L+HLTNY  E+I+VAI++ NVYV+ YRAGD +YF N++SA EA   + K  K
Sbjct: 65  LPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAGDTSYFFNEASATEAAKYVFKDAK 124

Query: 121 HE-RLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAF 180
            +  L Y GN          L+   G+ R+   + GL  + SAI ++F++N   S   A 
Sbjct: 125 RKVTLPYSGN-------YERLQIAAGKIRENIPL-GLPALDSAITTLFYYN-ANSAASAL 184

Query: 181 IVIIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGR 240
           +V+IQ   EA R+K+ E  + + V  +F       +LEN W+  S+ IQIA   +G+F  
Sbjct: 185 MVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFET 244

Query: 241 PVELRTIYNKPILVTN 255
           PV L    N+ + +TN
Sbjct: 245 PVVLINAQNQRVTITN 250

[Close]

BLAST of MC07g0190 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 156.0 bits (393), Expect = 5.7e-37
Identity = 99/256 (38.67%), Postives = 142/256 (55.47%), Query Frame = 0


Query: 1   LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 60
           LV    +L L+L   A E DVSF L GATS +Y  FI+NLR AL      +YDI +L   
Sbjct: 5   LVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALP-NERKLYDIPLLRSS 64

Query: 61  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSA--EADNVLLKGI 120
              S R+ L+HLTNY  E+I+VAI++ NVY++ YRAGD +YF N++SA   A  V    +
Sbjct: 65  LPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAM 124

Query: 121 KHERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAF 180
           +   L Y GN          L+   G+ R+   + GL  + SAI ++F++N   S   A 
Sbjct: 125 RKVTLPYSGN-------YERLQTAAGKIRENIPL-GLPALDSAITTLFYYN-ANSAASAL 184

Query: 181 IVIIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGR 240
           +V+IQ   EA R+K+ E  + + V  +F       +LEN W+  S+ IQIA   +G+F  
Sbjct: 185 MVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNGQFES 244

Query: 241 PVELRTIYNKPILVTN 255
           PV L    N+ + +TN
Sbjct: 245 PVVLINAQNQRVTITN 250

[Close]

BLAST of MC07g0190 vs. NCBI nr
Match: XP_022158192.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 317 bits (813), Expect = 1.02e-105
Identity = 170/251 (67.73%), Postives = 195/251 (77.69%), Query Frame = 0


Query: 4   FYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPPTTA 63
           FYFVLAL  GA A EADVSFS+LGATS+TY+QFI NLRS LT+RSP+VY I VL      
Sbjct: 6   FYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVG 65

Query: 64  STRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKHERL 123
           S RFIL+HLTNYNTESITVAI++VN+YVVAYRAG+NAYFLND+SAEA+NVL KGIKH RL
Sbjct: 66  SARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRL 125

Query: 124 SYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIVIIQ 183
            YKGN          L    G+     +  G SE+SS+IG+MFHHNVGTS+ RAFIVIIQ
Sbjct: 126 PYKGN-------YDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQ 185

Query: 184 IVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPVELR 243
            VLEA RFKY E  VSE+VKT FK DP F +LENRW+D SE IQIAQ R+GEF R +ELR
Sbjct: 186 SVLEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELR 245

Query: 244 TIYNKPILVTN 254
           T+ NKPI+VTN
Sbjct: 246 TVSNKPIIVTN 249

[Close]

BLAST of MC07g0190 vs. NCBI nr
Match: XP_022158193.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 309 bits (792), Expect = 1.58e-102
Identity = 169/254 (66.54%), Postives = 197/254 (77.56%), Query Frame = 0


Query: 1   LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 60
           L+ FYF LALYLGARA EADVSFS+LG+TS+TYKQFI N+RSALTV S +VYDI VL   
Sbjct: 3   LLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKAT 62

Query: 61  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKH 120
            T S RF+L+HLTNYN+ESI VAI++VNVYVVAYRAG+NAYFLND+SAEAD VL +GI H
Sbjct: 63  ATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINH 122

Query: 121 ERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIV 180
            +LSYKGN   ++      R          +  G SE+SS+IG+MFHHN GTSVPRAFIV
Sbjct: 123 VKLSYKGNYDGLETAAKISR--------EKIDLGFSEISSSIGNMFHHNAGTSVPRAFIV 182

Query: 181 IIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPV 240
           IIQ V EA RFKY E  VSE+VKT+FK DP F +L+NRW+D SE IQIAQ R GEF R V
Sbjct: 183 IIQSVSEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAV 242

Query: 241 ELRTIYNKPILVTN 254
           ELRT+ NKPILVT+
Sbjct: 243 ELRTVSNKPILVTD 248

[Close]

BLAST of MC07g0190 vs. NCBI nr
Match: BAG12408.1 (karasurin-H precursor [Trichosanthes kirilowii var. japonica])

HSP 1 Score: 292 bits (747), Expect = 1.11e-95
Identity = 161/254 (63.39%), Postives = 185/254 (72.83%), Query Frame = 0


Query: 1   LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 60
           LV FY VLALY+GA+ TEADV+FSLLG+TS TYKQFI NLR+ALTV SP V +I VL   
Sbjct: 3   LVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKGT 62

Query: 61  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKH 120
                RF+L+HLTNYN ESITVA+++VNVYVVAYRAG+NAYFLND+SAEA+NVL KGI H
Sbjct: 63  AAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGINH 122

Query: 121 ERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIV 180
            RL Y GN          L    GR     +  G SE+SSAIGSMFH+N GTSVP+AFIV
Sbjct: 123 VRLPYGGN-------YDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIV 182

Query: 181 IIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPV 240
           IIQ V EA RFKY +  VSE+V+T F  DP F +LEN W   SE IQIAQ R GEF RP+
Sbjct: 183 IIQTVSEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPI 242

Query: 241 ELRTIYNKPILVTN 254
           ELRT+ N P  VTN
Sbjct: 243 ELRTVSNTPTFVTN 249

[Close]

BLAST of MC07g0190 vs. NCBI nr
Match: P98184.1 (RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Bryonia dioica])

HSP 1 Score: 266 bits (680), Expect = 1.61e-85
Identity = 151/254 (59.45%), Postives = 173/254 (68.11%), Query Frame = 0


Query: 2   VAFYFVLALYLGARATE-ADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 61
           + FY VLALY+GA  TE  D++FSL+GAT  TYK FI NLR+ LTV +P VYDI VL   
Sbjct: 4   IGFYSVLALYVGAHVTEDVDINFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNA 63

Query: 62  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKH 121
                RF L+ LTNYN ES+TVA+++VNVYVVAYRAG+ AYFL D+S EA+NVL  GI H
Sbjct: 64  AAGLARFQLVTLTNYNGESVTVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINH 123

Query: 122 ERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIV 181
            RL Y GN          L    GR     +  G SE+SSAIG+MF HN GTSVPRAFIV
Sbjct: 124 VRLPYGGN-------YDGLETAAGRISRENIELGFSEISSAIGNMFRHNPGTSVPRAFIV 183

Query: 182 IIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPV 241
           IIQ V EA RFKY E  VSE+V T FK DP F +L+N W   SE IQIAQ R GEF RPV
Sbjct: 184 IIQTVSEAARFKYIEQRVSENVGTKFKPDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPV 243

Query: 242 ELRTIYNKPILVTN 254
           ELRT+ N P  VTN
Sbjct: 244 ELRTVSNTPTFVTN 250

[Close]

BLAST of MC07g0190 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 266 bits (679), Expect = 5.26e-85
Identity = 141/239 (59.00%), Postives = 176/239 (73.64%), Query Frame = 0


Query: 16  ATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPPTTASTRFILLHLTNY 75
           A  ++VSFSLLGAT KTY+QFI NLR+ALT+ S +VY+I VL     +S RFIL+HLTNY
Sbjct: 32  APNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNY 91

Query: 76  NTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKHERLSYKGNDFPIKET 135
             E+ITVAI++VNVY+VAY+AG+ AYFL D+S EA +VL KGIK E L YKGN       
Sbjct: 92  KNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGN------- 151

Query: 136 MTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIVIIQIVLEAVRFKYTE 195
              L    G+     +  G SE+ SAIG+M+H+N GTSVPRAFIV+IQ + EA RFKY E
Sbjct: 152 YDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIE 211

Query: 196 HGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPVELRTIYNKPILVTN 254
             VS++V+T FK DP F +LENRW+D SE +QIAQ R GEF RP+E+R++ NKPILVTN
Sbjct: 212 TKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTN 263

[Close]

BLAST of MC07g0190 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 317 bits (813), Expect = 4.95e-106
Identity = 170/251 (67.73%), Postives = 195/251 (77.69%), Query Frame = 0


Query: 4   FYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPPTTA 63
           FYFVLAL  GA A EADVSFS+LGATS+TY+QFI NLRS LT+RSP+VY I VL      
Sbjct: 6   FYFVLALCFGALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVG 65

Query: 64  STRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKHERL 123
           S RFIL+HLTNYNTESITVAI++VN+YVVAYRAG+NAYFLND+SAEA+NVL KGIKH RL
Sbjct: 66  SARFILVHLTNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRL 125

Query: 124 SYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIVIIQ 183
            YKGN          L    G+     +  G SE+SS+IG+MFHHNVGTS+ RAFIVIIQ
Sbjct: 126 PYKGN-------YDGLETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQ 185

Query: 184 IVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPVELR 243
            VLEA RFKY E  VSE+VKT FK DP F +LENRW+D SE IQIAQ R+GEF R +ELR
Sbjct: 186 SVLEAARFKYIEQRVSENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELR 245

Query: 244 TIYNKPILVTN 254
           T+ NKPI+VTN
Sbjct: 246 TVSNKPIIVTN 249

[Close]

BLAST of MC07g0190 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 309 bits (792), Expect = 7.66e-103
Identity = 169/254 (66.54%), Postives = 197/254 (77.56%), Query Frame = 0


Query: 1   LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 60
           L+ FYF LALYLGARA EADVSFS+LG+TS+TYKQFI N+RSALTV S +VYDI VL   
Sbjct: 3   LLPFYFFLALYLGARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKAT 62

Query: 61  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKH 120
            T S RF+L+HLTNYN+ESI VAI++VNVYVVAYRAG+NAYFLND+SAEAD VL +GI H
Sbjct: 63  ATGSARFLLVHLTNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINH 122

Query: 121 ERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIV 180
            +LSYKGN   ++      R          +  G SE+SS+IG+MFHHN GTSVPRAFIV
Sbjct: 123 VKLSYKGNYDGLETAAKISR--------EKIDLGFSEISSSIGNMFHHNAGTSVPRAFIV 182

Query: 181 IIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPV 240
           IIQ V EA RFKY E  VSE+VKT+FK DP F +L+NRW+D SE IQIAQ R GEF R V
Sbjct: 183 IIQSVSEAARFKYIEQRVSENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAV 242

Query: 241 ELRTIYNKPILVTN 254
           ELRT+ NKPILVT+
Sbjct: 243 ELRTVSNKPILVTD 248

[Close]

BLAST of MC07g0190 vs. ExPASy TrEMBL
Match: B1B626 (rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1)

HSP 1 Score: 292 bits (747), Expect = 5.37e-96
Identity = 161/254 (63.39%), Postives = 185/254 (72.83%), Query Frame = 0


Query: 1   LVAFYFVLALYLGARATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPP 60
           LV FY VLALY+GA+ TEADV+FSLLG+TS TYKQFI NLR+ALTV SP V +I VL   
Sbjct: 3   LVGFYLVLALYVGAQVTEADVNFSLLGSTSATYKQFIQNLRAALTVGSPTVENIPVLKGT 62

Query: 61  TTASTRFILLHLTNYNTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKH 120
                RF+L+HLTNYN ESITVA+++VNVYVVAYRAG+NAYFLND+SAEA+NVL KGI H
Sbjct: 63  AAGLARFLLVHLTNYNGESITVAVDVVNVYVVAYRAGNNAYFLNDASAEANNVLFKGINH 122

Query: 121 ERLSYKGNDFPIKETMTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIV 180
            RL Y GN          L    GR     +  G SE+SSAIGSMFH+N GTSVP+AFIV
Sbjct: 123 VRLPYGGN-------YDGLETAAGRISREKIYLGFSEISSAIGSMFHYNAGTSVPKAFIV 182

Query: 181 IIQIVLEAVRFKYTEHGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPV 240
           IIQ V EA RFKY +  VSE+V+T F  DP F +LEN W   SE IQIAQ R GEF RP+
Sbjct: 183 IIQTVSEAARFKYIQQRVSENVQTKFLPDPAFLSLENAWGGLSEQIQIAQYRGGEFARPI 242

Query: 241 ELRTIYNKPILVTN 254
           ELRT+ N P  VTN
Sbjct: 243 ELRTVSNTPTFVTN 249

[Close]

BLAST of MC07g0190 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 266 bits (679), Expect = 2.55e-85
Identity = 141/239 (59.00%), Postives = 176/239 (73.64%), Query Frame = 0


Query: 16  ATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPPTTASTRFILLHLTNY 75
           A  ++VSFSLLGAT KTY+QFI NLR+ALT+ S +VY+I VL     +S RFIL+HLTNY
Sbjct: 32  APNSNVSFSLLGATGKTYQQFIQNLRNALTINSKIVYNIPVLAATALSSARFILVHLTNY 91

Query: 76  NTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKHERLSYKGNDFPIKET 135
             E+ITVAI++VNVY+VAY+AG+ AYFL D+S EA +VL KGIK E L YKGN       
Sbjct: 92  KNETITVAIDVVNVYIVAYQAGNKAYFLKDASKEARDVLFKGIKQEILPYKGN------- 151

Query: 136 MTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIVIIQIVLEAVRFKYTE 195
              L    G+     +  G SE+ SAIG+M+H+N GTSVPRAFIV+IQ + EA RFKY E
Sbjct: 152 YDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAGTSVPRAFIVMIQTISEAARFKYIE 211

Query: 196 HGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPVELRTIYNKPILVTN 254
             VS++V+T FK DP F +LENRW+D SE +QIAQ R GEF RP+E+R++ NKPILVTN
Sbjct: 212 TKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQNRKGEFARPIEVRSVTNKPILVTN 263

[Close]

BLAST of MC07g0190 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 232 bits (591), Expect = 4.68e-72
Identity = 126/239 (52.72%), Postives = 170/239 (71.13%), Query Frame = 0


Query: 16  ATEADVSFSLLGATSKTYKQFITNLRSALTVRSPMVYDILVLYPPTTASTRFILLHLTNY 75
           +  A++SFSLLGAT++TYKQFI++LR ALTV S  VYDI VL        RFIL++LTNY
Sbjct: 31  SANANLSFSLLGATAQTYKQFISDLRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNY 90

Query: 76  NTESITVAINIVNVYVVAYRAGDNAYFLNDSSAEADNVLLKGIKHERLSYKGNDFPIKET 135
             ESITVAI+ V+VY+VAY+AG+ AYFL D S EA +VL KG KHE LSYKGN   ++  
Sbjct: 91  KGESITVAIDTVDVYIVAYQAGNKAYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERH 150

Query: 136 MTALRGLQGRFRDRTLISGLSEMSSAIGSMFHHNVGTSVPRAFIVIIQIVLEAVRFKYTE 195
                   G+     +  G SE+SS+IG+MFH+N G SVP++FIV+IQ++ EA RFKY E
Sbjct: 151 A-------GKISRENIDLGFSELSSSIGNMFHYNAGYSVPKSFIVMIQMLSEASRFKYIE 210

Query: 196 HGVSEHVKTSFKRDPTFFNLENRWTDFSEHIQIAQIRDGEFGRPVELRTIYNKPILVTN 254
               E+V++ FK DP + +LENRW++ SE +Q AQ   G+F  PV++R++ NKP+LV++
Sbjct: 211 ARNCENVESKFKPDPGYLSLENRWSNLSEQVQKAQKHGGKFEHPVQVRSVSNKPVLVSD 262

The following BLAST results are available for this feature:

BLAST of MC07g0190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.2e-71	59.45	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P24817	8.5e-41	40.55	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	1.4e-40	40.55	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P24478	8.8e-38	40.23	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P09989	5.7e-37	38.67	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]

BLAST of MC07g0190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158192.1	1.02e-105	67.73	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
XP_022158193.1	1.58e-102	66.54	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
BAG12408.1	1.11e-95	63.39	karasurin-H precursor [Trichosanthes kirilowii var. japonica]	[more]
P98184.1	1.61e-85	59.45	RecName: Full=Ribosome-inactivating protein bryodin II; AltName: Full=BD2; AltNa...	[more]
XP_022158191.1	5.26e-85	59.00	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]

BLAST of MC07g0190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWK4	4.95e-106	67.73	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	7.66e-103	66.54	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]
B1B626	5.37e-96	63.39	rRNA N-glycosidase OS=Trichosanthes kirilowii var. japonica OX=61011 PE=2 SV=1	[more]
A0A6J1E093	2.55e-85	59.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1IUB2	4.68e-72	52.72	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]

BLAST of MC07g0190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 210..227 score: 33.33 coord: 178..198 score: 46.62 coord: 79..94 score: 49.34 coord: 31..44 score: 42.86 coord: 148..162 score: 22.63
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 191..254 e-value: 4.5E-14 score: 54.1
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 20..190 e-value: 2.1E-30 score: 107.7
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 29..227 e-value: 1.0E-20 score: 74.3
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 20..250
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 20..250
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 20..254

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0190.1	MC07g0190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity