MC07g0159 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0159
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	rRNA N-glycosidase
Location	MC07: 3144651 .. 3145421 (-)
RNA-Seq Expression	MC07g0159
Synteny	MC07g0159

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAATCCATAGCACTTTCTCTTTTCATTCTCGCAATCTTTCTTGGCCCTCTTACTGCCAAGGGTGATGTTAGCTTTGATTTGTCCACAACTACCAAAAACTCGTATACATCTTTTATCAGAGACTTGAGGAATGCTCTTCCAACCAGATGGACGGTGTGCAATATATGTGTACCACCTTCTACCGTGTCGAGGTTAGAATGGTTCAGACTCTTCAATATCACCAATTATAACTACGAAACCATCACGGTGGCGGTAAATGTAACAAATGTTTATATCGCGGCGTATAGCACTGGTGCTATATCCTACTTTTTTTTAAGACACTTCTGCTGAAGCTCTCAGGCTCCTATTCAAAGATACTAAAAAGGTAACACTTCCATATTCGTGTAATTATGATAGGCTTCAGAATGTTGTAGGCAAAAATCGAGATTTTATCGAGCTTGGAGTCCCTAATTTAAGCAATGCCATTACAAACTTGTTGTACAACACCAACTACAAACTTACTGCAGCCGCACTACTCGTACTCATTCAGTCGACTGCGGAAGCTGCAAGATTTAAATATATTGAGCAAGAAGTTTCTCGACATGTTAACAACAATTTTTAAGTTTGGAAAACAATTTGAGTGCTCTGTCCAAACAAATCCAGATAGCAATTAGTAAAGGAAATGGACAATTTGACAAAGTTGTTGAGCTTGTAAACATGGATGGCAAACGAGTTAGTGTAACCAATACTTCTGCTGCAGTTGTAAAAAGCAATATTAGAAAACTG

mRNA sequence

ATGATGAAATCCATAGCACTTTCTCTTTTCATTCTCGCAATCTTTCTTGGCCCTCTTACTGCCAAGGGTGATGTTAGCTTTGATTTGTCCACAACTACCAAAAACTCGTATACATCTTTTATCAGAGACTTGAGGAATGCTCTTCCAACCAGATGGACGGTGTGCAATATATGTGTACCACCTTCTACCGTGTCGAGGTTAGAATGGTTCAGACTCTTCAATATCACCAATTATAACTACGAAACCATCACGGTGGCGGTAAATGTAACAAATGTTTATATCGCGGCGTATAGCACTGGTGCTATATCCTACTTTTTTGACACTTCTGCTGAAGCTCTCAGGCTCCTATTCAAAGATACTAAAAAGGTAACACTTCCATATTCGTGTAATTATGATAGGCTTCAGAATGTTGTAGGCAAAAATCGAGATTTTATCGAGCTTGGAGTCCCTAATTTAAGCAATGCCATTACAAACTTGTTGTACAACACCAACTACAAACTTACTGCAGCCGCACTACTCGTACTCATTCAGTCGACTGCGGAAGCTGCAAGATTTAAATATATTGAGCAAGAAGTTTCTCGACATGTTAACAACAATTTTAGTTTGGAAAACAATTTGAGTGCTCTGTCCAAACAAATCCAGATAGCAATTAGTAAAGGAAATGGACAATTTGACAAAGTTGTTGAGCTTGTAAACATGGATGGCAAACGAGTTAGTGTAACCAATACTTCTGCTGCAGTTGTAAAAAGCAATATTAGAAAACTG

Coding sequence (CDS)

ATGATGAAATCCATAGCACTTTCTCTTTTCATTCTCGCAATCTTTCTTGGCCCTCTTACTGCCAAGGGTGATGTTAGCTTTGATTTGTCCACAACTACCAAAAACTCGTATACATCTTTTATCAGAGACTTGAGGAATGCTCTTCCAACCAGATGGACGGTGTGCAATATATGTGTACCACCTTCTACCGTGTCGAGGTTAGAATGGTTCAGACTCTTCAATATCACCAATTATAACTACGAAACCATCACGGTGGCGGTAAATGTAACAAATGTTTATATCGCGGCGTATAGCACTGGTGCTATATCCTACTTTTTTGACACTTCTGCTGAAGCTCTCAGGCTCCTATTCAAAGATACTAAAAAGGTAACACTTCCATATTCGTGTAATTATGATAGGCTTCAGAATGTTGTAGGCAAAAATCGAGATTTTATCGAGCTTGGAGTCCCTAATTTAAGCAATGCCATTACAAACTTGTTGTACAACACCAACTACAAACTTACTGCAGCCGCACTACTCGTACTCATTCAGTCGACTGCGGAAGCTGCAAGATTTAAATATATTGAGCAAGAAGTTTCTCGACATGTTAACAACAATTTTAGTTTGGAAAACAATTTGAGTGCTCTGTCCAAACAAATCCAGATAGCAATTAGTAAAGGAAATGGACAATTTGACAAAGTTGTTGAGCTTGTAAACATGGATGGCAAACGAGTTAGTGTAACCAATACTTCTGCTGCAGTTGTAAAAAGCAATATTAGAAAACTG

Protein sequence

MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVPPSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFFDTSAEALRLLFKDTKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQSTAEAARFKYIEQEVSRHVNNNFSLENNLSALSKQIQIAISKGNGQFDKVVELVNMDGKRVSVTNTSAAVVKSNIRKL

Homology

[Close]

BLAST of MC07g0159 vs. ExPASy Swiss-Prot
Match: P56626 (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 302.8 bits (774), Expect = 3.8e-81
Identity = 171/259 (66.02%), Postives = 198/259 (76.45%), Query Frame = 0


Query: 5   IALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVPPSTV 64
           +ALS F LAI LG  TA GDVSFDLST TK SY+SFI  LR+ALPT+ TVC I + PST 
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  SRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKDTKKV 124
           S  +WFR FN+TNYN ET+TVAVNVTNVYI AY   A+SYFF DT AEA +L+F  TK V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 TLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQSTAEAA 184
            LPYS NYD+LQ+VVGK RD IELG+P LS+AITN++Y  +Y+ TAAALLVLIQ TAEAA
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVY-YDYQSTAAALLVLIQCTAEAA 180

Query: 185 RFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVNMDGK 244
           R+KYIEQ+VS H+++NF       SLEN   ALSKQIQIA   G+GQF+  VEL N DG 
Sbjct: 181 RYKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGT 240

Query: 245 RVSVTNTSAAVVKSNIRKL 256
           R SVTNTSA VVK NI+ L
Sbjct: 241 RFSVTNTSAGVVKGNIKLL 258

[Close]

BLAST of MC07g0159 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 225.7 bits (574), Expect = 5.9e-58
Identity = 136/263 (51.71%), Postives = 179/263 (68.06%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M+K + LS  I+AIF+G  TAKGDV+FDLST T  +YT FI D R  LP    V +I + 
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
            ST+S    F L N+T+Y YETI+VA++VTNVY+ AY T  +SYFF ++  EA  +LFK 
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+K+TLPY+ NY+ LQ    K R+ I+LG+P LS+AIT L Y  N +   +ALLVLIQ+T
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFY-YNAQSAPSALLVLIQTT 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIE+ V+++V  NF       SLEN  SALSKQI +A ++G G+F   V+L+ 
Sbjct: 181 AEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIK 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 256
             G+R  VTN  + VVK NI+ L
Sbjct: 241 PTGERFQVTNVDSDVVKGNIKLL 261

[Close]

BLAST of MC07g0159 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.2e-57
Identity = 135/263 (51.33%), Postives = 179/263 (68.06%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M+K + LS  I+AIF+G  TAKGDV+FDLST T  +YT FI D R  LP    V +I + 
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
            ST+S    F L ++T+Y YETI+VA++VTNVY+ AY T  +SYFF ++  EA  +LFK 
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+K+TLPY+ NY+ LQ    K R+ I+LG+P LS+AIT L Y  N +   +ALLVLIQ+T
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFY-YNAQSAPSALLVLIQTT 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIE+ V+++V  NF       SLEN  SALSKQI +A ++G G+F   V+L+ 
Sbjct: 181 AEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIK 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 256
             G+R  VTN  + VVK NI+ L
Sbjct: 241 PTGERFQVTNVDSDVVKGNIKLL 261

[Close]

BLAST of MC07g0159 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 219.5 bits (558), Expect = 4.2e-56
Identity = 141/265 (53.21%), Postives = 178/265 (67.17%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M+K + L L IL IFL   T +GDVSF LS  T  SY  FI++LR ALP    V NI + 
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYERKVYNIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFFD--TSAEALRLLFK 120
            S++S    + L ++TNY  ETI+VAV+VTNVYI  Y  G +SYFF+  ++ EA + +FK
Sbjct: 61  RSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAGDVSYFFNEASATEAAKFVFK 120

Query: 121 DT-KKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQ 180
           D  KKVTLPYS NY+RLQ   GK R+ I LG+P L +AIT L Y T     A+ALLVLIQ
Sbjct: 121 DAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYT-ASSAASALLVLIQ 180

Query: 181 STAEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVEL 240
           STAE+AR+K+IEQ++ + V+  F       SLENN SALSKQIQIA S  NGQF+  V L
Sbjct: 181 STAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIA-STNNGQFESPVVL 240

Query: 241 VNMDGKRVSVTNTSAAVVKSNIRKL 256
           ++ + +RVS+TN SA VV SNI  L
Sbjct: 241 IDGNNQRVSITNASARVVTSNIALL 263

[Close]

BLAST of MC07g0159 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 4.4e-53
Identity = 131/265 (49.43%), Postives = 175/265 (66.04%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M++ + LSL IL +FL     +GDVSF LS  T +SY  FI +LR ALP    + +I + 
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFFD--TSAEALRLLFK 120
            S++   + + L ++TNY  ETI+VA++VTNVYI  Y  G  SYFF+  ++ EA + +FK
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 DT-KKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQ 180
           D  +KVTLPYS NY+RLQ   GK R+ I LG+P L +AIT L Y  N    A+AL+VLIQ
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFY-YNANSAASALMVLIQ 180

Query: 181 STAEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVEL 240
           ST+EAAR+K+IEQ++ + V+  F       SLEN+ SALSKQIQIA S  NGQF+  V L
Sbjct: 181 STSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIA-STNNGQFESPVVL 240

Query: 241 VNMDGKRVSVTNTSAAVVKSNIRKL 256
           +N   +RV++TN  A VV SNI  L
Sbjct: 241 INAQNQRVTITNVDAGVVTSNIALL 263

[Close]

BLAST of MC07g0159 vs. NCBI nr
Match: XP_022156709.1 (type I ribosome-inactivating protein trichoanguina-like [Momordica charantia])

HSP 1 Score: 389 bits (998), Expect = 9.01e-134
Identity = 210/263 (79.85%), Postives = 227/263 (86.31%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           MMKSIALS FIL IFLGP TAKGDVSFDLST TKNSYTSFIRDLR+ALPTR TVCNI V 
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
           PST S L+WFR F++TNYNY+TITVAVNVTNVYIAAYSTGA+SYFF DTSAEA RLLF  
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+KVTLPYS NYDRLQ++VGKNRD IELG+P LSNAITNL YN NYKLTA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIEQEVS+HV++NF       SLEN+  ALSKQIQIA SKG GQFDK VEL+ 
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
           +DGKRVSVTNTS  VVKSNI+ L
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLL 263

[Close]

BLAST of MC07g0159 vs. NCBI nr
Match: P56626.2 (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Trichosanthes anguina] >AAD02686.1 trichoanguin [Trichosanthes cucumerina])

HSP 1 Score: 303 bits (775), Expect = 9.68e-100
Identity = 171/258 (66.28%), Postives = 197/258 (76.36%), Query Frame = 0


Query: 6   ALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVPPSTVS 65
           ALS F LAI LG  TA GDVSFDLST TK SY+SFI  LR+ALPT+ TVC I + PST S
Sbjct: 2   ALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTAS 61

Query: 66  RLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKDTKKVT 125
             +WFR FN+TNYN ET+TVAVNVTNVYI AY   A+SYFF DT AEA +L+F  TK V 
Sbjct: 62  GSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTVK 121

Query: 126 LPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQSTAEAAR 185
           LPYS NYD+LQ+VVGK RD IELG+P LS+AITN++Y  +Y+ TAAALLVLIQ TAEAAR
Sbjct: 122 LPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYY-DYQSTAAALLVLIQCTAEAAR 181

Query: 186 FKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVNMDGKR 245
           +KYIEQ+VS H+++NF       SLEN   ALSKQIQIA   G+GQF+  VEL N DG R
Sbjct: 182 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 241

Query: 246 VSVTNTSAAVVKSNIRKL 255
            SVTNTSA VVK NI+ L
Sbjct: 242 FSVTNTSAGVVKGNIKLL 258

[Close]

BLAST of MC07g0159 vs. NCBI nr
Match: XP_023005055.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima])

HSP 1 Score: 293 bits (749), Expect = 7.98e-96
Identity = 166/263 (63.12%), Postives = 202/263 (76.81%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M K IA++ F++AI L   T K D++FDLST TK SY++FI  LRNALPT  TVCNI + 
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
           PST S L WF  F ITNYNY+TITVAVNVTNVYI AY   A SYFF DT AEA +LLF +
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+KVTLP++ NYD+LQ++VGK RD IELG+P LS A+TN++Y  N +LTAAALLVLIQS+
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYY-NKQLTAAALLVLIQSS 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIEQ+V++H+++ F       SLENN  ALSKQIQIA S G GQF   VEL++
Sbjct: 181 AEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIS 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
            +G R SVT+TSA VV+SNI+ L
Sbjct: 241 PNGTRFSVTSTSAGVVQSNIKLL 262

[Close]

BLAST of MC07g0159 vs. NCBI nr
Match: XP_023513430.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 284 bits (727), Expect = 1.73e-92
Identity = 162/263 (61.60%), Postives = 197/263 (74.90%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M K IA+S F+LAI L   T K D++ DLST TK SY++FI  +RNALPT+ TVCNI + 
Sbjct: 1   MNKLIAISSFLLAISLISPTLKADIALDLSTATKASYSAFITHIRNALPTKATVCNIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
           PST + L  F  F ITNYNY+T+ VAVNVTNVYI AY  GA SYFF DT A+A +LLF  
Sbjct: 61  PSTPTGLASFTFFTITNYNYQTVEVAVNVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAG 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+KVTLP++ NYD+LQ++VGK RD +ELG+P LS A+TN++Y  N +LTAAALLVLIQST
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYY-NKQLTAAALLVLIQST 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIEQ+V++H+++ F       SLENN  ALSKQIQIA S G GQF   VEL+ 
Sbjct: 181 AEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIG 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
             G R SVTNTSA VV+ NI+ L
Sbjct: 241 PTGTRFSVTNTSAGVVQGNIKLL 262

[Close]

BLAST of MC07g0159 vs. NCBI nr
Match: KAG6592904.1 (Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025309.1 Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 279 bits (713), Expect = 2.30e-90
Identity = 157/263 (59.70%), Postives = 198/263 (75.29%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M K IA++ F+LAI L   T K D++FDLST TK SY++FI  LRNALPT+ TVCNI + 
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
           PST + L WF  F ITNYNY+T+ VAVNV+NVYI AY   A SYFF DT A+A +LLF  
Sbjct: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+KVTLP++ NYD+LQ++VGK RD +ELG+P LS A+TN++Y  N + TAAALLVLIQST
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYY-NKQPTAAALLVLIQST 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIEQ+V+++++  F       SLENN  ALSKQIQIA S G+GQF   VEL++
Sbjct: 181 AEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELIS 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
            +G R  VT+TS+ VV+ NI+ L
Sbjct: 241 PNGTRFRVTSTSSGVVQGNIKLL 262

[Close]

BLAST of MC07g0159 vs. ExPASy TrEMBL
Match: A0A6J1DUC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1)

HSP 1 Score: 389 bits (998), Expect = 4.36e-134
Identity = 210/263 (79.85%), Postives = 227/263 (86.31%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           MMKSIALS FIL IFLGP TAKGDVSFDLST TKNSYTSFIRDLR+ALPTR TVCNI V 
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
           PST S L+WFR F++TNYNY+TITVAVNVTNVYIAAYSTGA+SYFF DTSAEA RLLF  
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+KVTLPYS NYDRLQ++VGKNRD IELG+P LSNAITNL YN NYKLTA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIEQEVS+HV++NF       SLEN+  ALSKQIQIA SKG GQFDK VEL+ 
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
           +DGKRVSVTNTS  VVKSNI+ L
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLL 263

[Close]

BLAST of MC07g0159 vs. ExPASy TrEMBL
Match: A0A6J1KWC4 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1)

HSP 1 Score: 293 bits (749), Expect = 3.87e-96
Identity = 166/263 (63.12%), Postives = 202/263 (76.81%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M K IA++ F++AI L   T K D++FDLST TK SY++FI  LRNALPT  TVCNI + 
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
           PST S L WF  F ITNYNY+TITVAVNVTNVYI AY   A SYFF DT AEA +LLF +
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+KVTLP++ NYD+LQ++VGK RD IELG+P LS A+TN++Y  N +LTAAALLVLIQS+
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYY-NKQLTAAALLVLIQSS 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIEQ+V++H+++ F       SLENN  ALSKQIQIA S G GQF   VEL++
Sbjct: 181 AEAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIS 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
            +G R SVT+TSA VV+SNI+ L
Sbjct: 241 PNGTRFSVTSTSAGVVQSNIKLL 262

[Close]

BLAST of MC07g0159 vs. ExPASy TrEMBL
Match: A0A6J1H951 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1)

HSP 1 Score: 278 bits (711), Expect = 2.24e-90
Identity = 159/263 (60.46%), Postives = 199/263 (75.67%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M K IA++ F+LAI L   T K D++ DLST TK +Y++FI  LRNALPT+ TVCNI + 
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFF-DTSAEALRLLFKD 120
           PST + L  F  F ITNYNYET+ VAVNV+NVYI AY   A SYFF DTSA+A +LLF++
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+K+TLP++ NYDRLQ VVGK RD IELG+P LS A+TN++Y  N +LTAAALLVLIQST
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYY-NKQLTAAALLVLIQST 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIEQ+V+++++  F       SLENN  ALSKQIQIA S G+GQF   VEL++
Sbjct: 181 AEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELIS 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
            +G R  VT+TS+ VV+ NI+ L
Sbjct: 241 PNGTRFRVTSTSSGVVQGNIKLL 262

[Close]

BLAST of MC07g0159 vs. ExPASy TrEMBL
Match: A0A6J1DR40 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1)

HSP 1 Score: 270 bits (689), Expect = 4.68e-87
Identity = 158/263 (60.08%), Postives = 193/263 (73.38%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           M K   LSL +LAI LGPL+A+GDVSF LS   K SYT+FI+ LR+A+PT  TVC I V 
Sbjct: 1   MRKLTTLSL-LLAILLGPLSAEGDVSFQLSGANKKSYTNFIKQLRDAVPTTTTVCKIPVL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFFD-TSAEALRLLFKD 120
           PST S  + F   ++TNY  +T+ VA+N+TNVYI AY    +SYFFD  S EA ++LF+ 
Sbjct: 61  PSTASGPKSFTFVHLTNYADQTVVVAINITNVYIVAYHIDHVSYFFDDASPEAYKVLFEG 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T + TLPYS NYD+LQ++VGK RD IELG+P LS+AITNL+Y   YK TAAALLVLIQS 
Sbjct: 121 TDRKTLPYSSNYDKLQSIVGKKRDLIELGLPALSSAITNLVYY-EYKSTAAALLVLIQSV 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAAR+KYIEQE+S HV++ F       SLENN  ALSKQIQIA +KGNGQF+K VEL+N
Sbjct: 181 AEAARYKYIEQEISTHVDDKFKPDQAIISLENNWGALSKQIQIA-AKGNGQFEKPVELIN 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
            +    SVTN S+ VV SNI+ L
Sbjct: 241 PNNTPFSVTNVSSLVVTSNIKLL 260

[Close]

BLAST of MC07g0159 vs. ExPASy TrEMBL
Match: A0A6J1DRC7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023553 PE=3 SV=1)

HSP 1 Score: 227 bits (578), Expect = 2.49e-70
Identity = 138/263 (52.47%), Postives = 178/263 (67.68%), Query Frame = 0


Query: 1   MMKSIALSLFILAIFLGPLTAKGDVSFDLSTTTKNSYTSFIRDLRNALPTRWTVCNICVP 60
           MMK + LS  I+AIF+G  TAKGDV+FDLST T  +YT FI D R  LP    V +I + 
Sbjct: 1   MMKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTVSRLEWFRLFNITNYNYETITVAVNVTNVYIAAYSTGAISYFFDTSA-EALRLLFKD 120
            ST+S    F L N+T+Y YETI+VA++VTNVY+ AY T  +SYFF  S  EA  +LFK 
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TKKVTLPYSCNYDRLQNVVGKNRDFIELGVPNLSNAITNLLYNTNYKLTAAALLVLIQST 180
           T+K+TLPY+ NY+ LQ    K R+ I+LG+P LS+AIT L Y  N +   +ALLVLIQ+T
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYY-NAQSAPSALLVLIQTT 180

Query: 181 AEAARFKYIEQEVSRHVNNNF-------SLENNLSALSKQIQIAISKGNGQFDKVVELVN 240
           AEAARFKYIE+ V+++V  NF       SLEN  SALSKQI +A ++G G+F   V+L+ 
Sbjct: 181 AEAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIK 240

Query: 241 MDGKRVSVTNTSAAVVKSNIRKL 255
             G+R  VTN  + VVK NI+ L
Sbjct: 241 PTGERFQVTNVDSDVVKGNIKLL 261

The following BLAST results are available for this feature:

BLAST of MC07g0159 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P56626	3.8e-81	66.02	Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5...	[more]
P24817	5.9e-58	51.71	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P29339	2.2e-57	51.33	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P33185	4.2e-56	53.21	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
P09989	4.4e-53	49.43	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]

BLAST of MC07g0159 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156709.1	9.01e-134	79.85	type I ribosome-inactivating protein trichoanguina-like [Momordica charantia]	[more]
P56626.2	9.68e-100	66.28	RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt...	[more]
XP_023005055.1	7.98e-96	63.12	type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima]	[more]
XP_023513430.1	1.73e-92	61.60	type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. p...	[more]
KAG6592904.1	2.30e-90	59.70	Type I ribosome-inactivating protein trichoanguina, partial [Cucurbita argyrospe...	[more]

BLAST of MC07g0159 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUC7	4.36e-134	79.85	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023554 PE=3 SV=1	[more]
A0A6J1KWC4	3.87e-96	63.12	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111498162 PE=3 SV=1	[more]
A0A6J1H951	2.24e-90	60.46	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111461188 PE=3 SV=1	[more]
A0A6J1DR40	4.68e-87	60.08	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023561 PE=3 SV=1	[more]
A0A6J1DRC7	2.49e-70	52.47	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023553 PE=3 SV=1	[more]

BLAST of MC07g0159 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 82..97 score: 48.68 coord: 35..48 score: 44.36 coord: 172..192 score: 65.54 coord: 142..156 score: 44.56
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 184..255 e-value: 1.2E-13 score: 52.9
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 24..183 e-value: 2.2E-37 score: 130.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..199 e-value: 2.0E-23 score: 83.1
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 24..249
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 24..249
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 176..192
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 24..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0159.1	MC07g0159.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity