MC06g_new0442 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g_new0442
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Poly(A)-specific ribonuclease
Location	MC06: 19862490 .. 19863613 (+)
RNA-Seq Expression	MC06g_new0442
Synteny	MC06g_new0442

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGACATTCGGAAAGTCTCCGACAGCTCCAAGATCCGTCCACCAGAATCGAGAGGTTTGGTCTTACAATCTACACAAAGAGTTAGAGATTCTAAATGAGTATCTTCCAGAGTTTCCAATAGTCGCCATTGATACAGAGTTTCCAGGTATCGGGTTGCTTAAGGATAATCGAATCTATTTTCTAAGTTTAGCTGGAAAATATTTTTATGCTCGCAAGCTCGATGATGAACCAGGATTTCTCCGGTACACTCCGAAAGGTGTTTCGGAGGAAGAATTCTACGATTGTCTCCGATTTAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCAAATCAAAATGGAAATGTAGCGATGACTTGGGAGTTTAATTTCAGAGATTTTGATCACATTAGTGACATGGAAGGCGTTTCTTCACTGGACTCGTTTCTTAAGAACAAGGGGTTCGATTTCTACAAATTGAAGAGAGACGGAATAGCTCTAGAAGATTTCACTACCCTATTTCTACCGATTTGTCGTAGTGGTAGAATCGAGAGATGGATTACATTTCATGGCTTCTACGACATCGCTTATTTACTGAAGCTGCTCAAAATCAAATACATACCAATTTCTATGGCAACGTTTGCGGCTACTGCTCAACATCTATTAGGTACTAATGAATCGTCAATCAACTTAAAAGCTTAATTTGATGTGTTACGATAAATTTAATATTTAATATTTACTTGATAGGTACTGTCAGCGATTTGAAGCATATGTGTCGCTATTGTGACGGATTGCTCGACGGCGACCTAGGACTAAAAAAATTGGCTAAGCTGCTCGACGTAAAACGAATAGGCATAGCACATTTTGCCGGTTCCGATAGTTTGCTCACTGCTGCAGTATACACAAAGATGAAGCAAATATTCAAGGTTCCGGTAGAGCAACGTGAAGGGTTTCTCTACGGCTTGCCTCACCGGATTCAAGCTTTTCGGCCAATCGTGAAGATAGTTGATCCGTCTGCCATGACTCCTCCTCCTCCATGTCTGCCAACATTGTGTTTTGGTAGCTTTCCGCCGTTTTTCATTGATATTCACTCTAGTAAGTTAAAGGGTCATTCAGTTACAGTGAGTTGA

mRNA sequence

ATGGAAAAGACATTCGGAAAGTCTCCGACAGCTCCAAGATCCGTCCACCAGAATCGAGAGGTTTGGTCTTACAATCTACACAAAGAGTTAGAGATTCTAAATGAGTATCTTCCAGAGTTTCCAATAGTCGCCATTGATACAGAGTTTCCAGGTATCGGGTTGCTTAAGGATAATCGAATCTATTTTCTAAGTTTAGCTGGAAAATATTTTTATGCTCGCAAGCTCGATGATGAACCAGGATTTCTCCGGTACACTCCGAAAGGTGTTTCGGAGGAAGAATTCTACGATTGTCTCCGATTTAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCAAATCAAAATGGAAATGTAGCGATGACTTGGGAGTTTAATTTCAGAGATTTTGATCACATTAGTGACATGGAAGGCGTTTCTTCACTGGACTCGTTTCTTAAGAACAAGGGGTTCGATTTCTACAAATTGAAGAGAGACGGAATAGCTCTAGAAGATTTCACTACCCTATTTCTACCGATTTGTCGTAGTGGTAGAATCGAGAGATGGATTACATTTCATGGCTTCTACGACATCGCTTATTTACTGAAGCTGCTCAAAATCAAATACATACCAATTTCTATGGCAACGTTTGCGGCTACTGCTCAACATCTATTAGGTACTGTCAGCGATTTGAAGCATATGTGTCGCTATTGTGACGGATTGCTCGACGGCGACCTAGGACTAAAAAAATTGGCTAAGCTGCTCGACGTAAAACGAATAGGCATAGCACATTTTGCCGGTTCCGATAGTTTGCTCACTGCTGCAGTATACACAAAGATGAAGCAAATATTCAAGGTTCCGGTAGAGCAACGTGAAGGGTTTCTCTACGGCTTGCCTCACCGGATTCAAGCTTTTCGGCCAATCGTGAAGATAGTTGATCCGTCTGCCATGACTCCTCCTCCTCCATGTCTGCCAACATTGTGTTTTGGTAGCTTTCCGCCGTTTTTCATTGATATTCACTCTAGTAAGTTAAAGGGTCATTCAGTTACAGTGAGTTGA

Coding sequence (CDS)

ATGGAAAAGACATTCGGAAAGTCTCCGACAGCTCCAAGATCCGTCCACCAGAATCGAGAGGTTTGGTCTTACAATCTACACAAAGAGTTAGAGATTCTAAATGAGTATCTTCCAGAGTTTCCAATAGTCGCCATTGATACAGAGTTTCCAGGTATCGGGTTGCTTAAGGATAATCGAATCTATTTTCTAAGTTTAGCTGGAAAATATTTTTATGCTCGCAAGCTCGATGATGAACCAGGATTTCTCCGGTACACTCCGAAAGGTGTTTCGGAGGAAGAATTCTACGATTGTCTCCGATTTAATGTGAACCGATTGAACATACTCCAACTCGGTCTCACACTCGCAAATCAAAATGGAAATGTAGCGATGACTTGGGAGTTTAATTTCAGAGATTTTGATCACATTAGTGACATGGAAGGCGTTTCTTCACTGGACTCGTTTCTTAAGAACAAGGGGTTCGATTTCTACAAATTGAAGAGAGACGGAATAGCTCTAGAAGATTTCACTACCCTATTTCTACCGATTTGTCGTAGTGGTAGAATCGAGAGATGGATTACATTTCATGGCTTCTACGACATCGCTTATTTACTGAAGCTGCTCAAAATCAAATACATACCAATTTCTATGGCAACGTTTGCGGCTACTGCTCAACATCTATTAGGTACTGTCAGCGATTTGAAGCATATGTGTCGCTATTGTGACGGATTGCTCGACGGCGACCTAGGACTAAAAAAATTGGCTAAGCTGCTCGACGTAAAACGAATAGGCATAGCACATTTTGCCGGTTCCGATAGTTTGCTCACTGCTGCAGTATACACAAAGATGAAGCAAATATTCAAGGTTCCGGTAGAGCAACGTGAAGGGTTTCTCTACGGCTTGCCTCACCGGATTCAAGCTTTTCGGCCAATCGTGAAGATAGTTGATCCGTCTGCCATGACTCCTCCTCCTCCATGTCTGCCAACATTGTGTTTTGGTAGCTTTCCGCCGTTTTTCATTGATATTCACTCTAGTAAGTTAAAGGGTCATTCAGTTACAGTGAGTTGA

Protein sequence

MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRIERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAFRPIVKIVDPSAMTPPPPCLPTLCFGSFPPFFIDIHSSKLKGHSVTVS

Homology

[Close]

BLAST of MC06g_new0442 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.5e-43
Identity = 111/294 (37.76%), Postives = 155/294 (52.72%), Query Frame = 0


Query: 17  QNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGI-----GLLKDNRIYFLSLAGKYFY 76
           Q REVW+ NL +E++++ + + +FP VA+DTEFPGI     G  K N  Y          
Sbjct: 11  QIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY---------- 70

Query: 77  ARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVA-------MT 136
                                  Y+ L+ NVN L ++QLGLT +N+ GN+          
Sbjct: 71  ----------------------HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCI 130

Query: 137 WEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRI--- 196
           W+FNFR+FD  SD+  + S++  LK  G D  K   DGI  + F  L +    SG +   
Sbjct: 131 WQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKNTLDGIDSKRFAELLM---SSGIVLNE 190

Query: 197 -ERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 256
              W+TFH  YD  YLLKLL  + +P S   F         TV D+KH+ ++C+ L    
Sbjct: 191 NVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLINVYFPTVYDIKHLMKFCNSLHG-- 250

Query: 257 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKV-PVEQREGFLYGL 294
            GL KLA+LL+V+R+GI H AGSDSLLT+  + K+K+ F V P+ +  G LYGL
Sbjct: 251 -GLNKLAELLEVERVGICHQAGSDSLLTSCTFRKLKENFFVGPLHKYSGVLYGL 265

[Close]

BLAST of MC06g_new0442 vs. ExPASy Swiss-Prot
Match: Q9S9P2 (Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=CAF1-2 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.3e-41
Identity = 103/285 (36.14%), Postives = 155/285 (54.39%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           REVW++NL +E+ ++ + + +FP VA+DTEFPGI                    + +   
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGI------------------VCKTVTAN 76

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNV-------AMTWEFNFRD 138
           P      P  +  E  YD L+ NVN L ++QLGLTL+++ GN+          W+FNFR+
Sbjct: 77  P---NPNPYSIHYEYNYDTLKANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFRE 136

Query: 139 FDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFL--PICRSGRIERWITFHG 198
           F+ ISDM  + S++  L+    D  K    G+  + F  L +   +  + +I  W+TFH 
Sbjct: 137 FNVISDMFALDSIE-LLRKSAIDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHC 196

Query: 199 FYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKL 258
            YD  YLLKLL  K +P  ++ F    +     V D+K++  +C  L     GL+K+A+L
Sbjct: 197 GYDFGYLLKLLSGKELPEEISDFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAEL 256

Query: 259 LDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKV-PVEQREGFLYGL 294
           L VKR+GI+H AGSDSLLT   + KMK+ F    + +  GFL+GL
Sbjct: 257 LGVKRVGISHQAGSDSLLTLRTFIKMKEFFFTGSLLKYSGFLFGL 275

[Close]

BLAST of MC06g_new0442 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 168.7 bits (426), Expect = 1.2e-40
Identity = 107/295 (36.27%), Postives = 151/295 (51.19%), Query Frame = 0


Query: 17  QNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGI-----GLLKDNRIYFLSLAGKYFY 76
           Q REVW+ NL  E+ ++ E + +FP VA+DTEFPGI     G  K N  Y          
Sbjct: 11  QIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRPVGTFKTNTEY---------- 70

Query: 77  ARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVA--------M 136
                                  Y+ L+ NVN L ++QLGLT +++ GN+          
Sbjct: 71  ----------------------HYETLKTNVNILKMIQLGLTFSDEKGNLPTCGTDNKYC 130

Query: 137 TWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRI-- 196
            W+FNFR+FD  SD+    S++  L+  G DF K    GI  + F  L +    SG +  
Sbjct: 131 IWQFNFREFDLESDIYATDSIE-LLRQSGIDFVKNNEFGIDSKRFAELLM---SSGIVLN 190

Query: 197 --ERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDG 256
               W+TFH  YD  YLLKLL  + +P +   F          V D+KH+ ++C+ L   
Sbjct: 191 ENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGFFEMISVYFPRVYDIKHLMKFCNSLHG- 250

Query: 257 DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKV-PVEQREGFLYGL 294
             GL KLA+LLDV+R+GI H AGSDSLLT+  + K+++ F +  +E+  G LYGL
Sbjct: 251 --GLNKLAELLDVERVGICHQAGSDSLLTSCTFRKLQENFFIGSMEKYSGVLYGL 266

[Close]

BLAST of MC06g_new0442 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 5.8e-40
Identity = 101/291 (34.71%), Postives = 156/291 (53.61%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           REVW+ NL  E E+++E + ++P +++DTEFPG+    D                     
Sbjct: 20  REVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSD--------------------- 79

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNV---------AMTWEFNF 138
              LR+T    + ++ Y  L+ NV+ L+++Q+GLTL++ NGN+            WEFNF
Sbjct: 80  ---LRFT----NPDDLYTLLKANVDALSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNF 139

Query: 139 RDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLP---ICRSGRIERWIT 198
           RDFD   D     S++  L+ +G DF +  RDG+  E F  L +    +C       W+T
Sbjct: 140 RDFDVARDAHAPDSIE-LLRRQGIDFERNCRDGVESERFAELMMSSGLVCNEE--VSWVT 199

Query: 199 FHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLG-TVSDLKHMCRYCDGLLDGDLGLKK 258
           FH  YD  YL+K+L  + +P ++  F    + L G  V D+KHM ++C+  L G  GL +
Sbjct: 200 FHSAYDFGYLMKILTRRELPGALGEFKRVMRVLFGERVYDVKHMMKFCERRLFG--GLDR 259

Query: 259 LAKLLDVKR-IGIAHFAGSDSLLTAAVYTKMKQIFKVP--VEQREGFLYGL 294
           +A+ L+V R +G  H AGSDSLLT   + +M+ ++ V    E+  G LYGL
Sbjct: 260 VARTLEVNRAVGKCHQAGSDSLLTWHAFQRMRDLYFVQDGPEKHAGVLYGL 277

[Close]

BLAST of MC06g_new0442 vs. ExPASy Swiss-Prot
Match: Q9LEU4 (Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=CAF1-10 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.4e-38
Identity = 102/285 (35.79%), Postives = 148/285 (51.93%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           REVW YNL +E  ++ E + +F  +A+DTEFPG+          L     + Y   L+  
Sbjct: 13  REVWDYNLVEEFALIREIVDKFSYIAMDTEFPGV---------VLKPVATFKYNNDLN-- 72

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVA-------MTWEFNFRD 138
                           Y  L+ NV+ L ++Q+GLT +++NGN+          W+FNFR+
Sbjct: 73  ----------------YRTLKENVDLLKLIQVGLTFSDENGNLPTCGTDKFCIWQFNFRE 132

Query: 139 FDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLP--ICRSGRIERWITFHG 198
           F+   D+    S++  L+  G DF K    GI +  F  L +   I  +  I  W+TFHG
Sbjct: 133 FNIGEDIYASESIE-LLRQCGIDFKKNIEKGIDVVRFGELMMSSGIVLNDAIS-WVTFHG 192

Query: 199 FYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKL 258
            YD  YL+KLL  K +P+  A F         TV D+KH+  +C+GL     GL +LA+L
Sbjct: 193 GYDFGYLVKLLTCKELPLKQADFFKLLYVYFPTVYDIKHLMTFCNGLFG---GLNRLAEL 252

Query: 259 LDVKRIGIAHFAGSDSLLTAAVYTKMKQ-IFKVPVEQREGFLYGL 294
           + V+R+GI H AGSDSLLT   + K+K+  F    E+  G LYGL
Sbjct: 253 MGVERVGICHQAGSDSLLTLGSFRKLKERYFPGSTEKYTGVLYGL 265

[Close]

BLAST of MC06g_new0442 vs. NCBI nr
Match: XP_022154798.1 (probable CCR4-associated factor 1 homolog 6 [Momordica charantia])

HSP 1 Score: 707 bits (1824), Expect = 4.99e-257
Identity = 344/347 (99.14%), Postives = 346/347 (99.71%), Query Frame = 0


Query: 1   MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI 60
           MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI
Sbjct: 1   MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI 60

Query: 61  YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN 120
           YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN
Sbjct: 61  YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN 120

Query: 121 VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR 180
           VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR
Sbjct: 121 VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR 180

Query: 181 IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 240
           IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD
Sbjct: 181 IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 240

Query: 241 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAF 300
           LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQ+FKVPVEQREGFLYGLPHRIQAF
Sbjct: 241 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQAF 300

Query: 301 RPIVKIVDPSAMTPPPPCLPTLCFGSFPPFFIDIHSSKLKGHSVTVS 347
           +PIVKIVDPSAMTPPPP LPTLCFGSFPPFFIDIHSSKLKGHSVTVS
Sbjct: 301 QPIVKIVDPSAMTPPPPRLPTLCFGSFPPFFIDIHSSKLKGHSVTVS 347

[Close]

BLAST of MC06g_new0442 vs. NCBI nr
Match: XP_022146384.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 477 bits (1228), Expect = 2.33e-166
Identity = 249/351 (70.94%), Postives = 271/351 (77.21%), Query Frame = 0


Query: 1   MEKTF-GKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNR 60
           MEK    KS  APRS+  NREVWS NL +ELE+LNEYLPEFP+V ID EFPG        
Sbjct: 19  MEKAIVRKSTAAPRSICHNREVWSLNLREELELLNEYLPEFPVVVIDMEFPG-------- 78

Query: 61  IYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNG 120
                                FL  TPKG+SEEEFY CLRFNVNRLNILQLGLTLA+Q G
Sbjct: 79  ---------------------FLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKG 138

Query: 121 NVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSG 180
           N+AMTWEFNFRDFDH+SDME VSSLDSFLKNKGFDFYKLK+ GIA EDFTTLFLPICRSG
Sbjct: 139 NIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSG 198

Query: 181 RIERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDG 240
           +I+RWITFHGFYDI YLLKLLKIK IPISMATFA TAQHLLGTVSDLKHM RYCDGLLDG
Sbjct: 199 QIKRWITFHGFYDITYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDG 258

Query: 241 DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQA 300
           +LGLKKLAKLLDVKRI  AHFA SD+L T +VYTKMKQIFK+P+EQ EGFLYGLP+ I  
Sbjct: 259 NLGLKKLAKLLDVKRIDNAHFACSDNLFTTSVYTKMKQIFKLPLEQCEGFLYGLPYPING 318

Query: 301 FRPIVKIVDPSAMTPPPPCLPT----LCFGSFPPFFIDIHSSKLKGHSVTV 346
           F+ + KI+DPS MTPPPP LP+    L F S  P  + I SSKL G SV V
Sbjct: 319 FQSVAKIIDPSTMTPPPP-LPSKFSILSFCSSTPLLVGIKSSKLNGPSVIV 339

[Close]

BLAST of MC06g_new0442 vs. NCBI nr
Match: XP_022153955.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 393 bits (1009), Expect = 8.96e-134
Identity = 206/315 (65.40%), Postives = 238/315 (75.56%), Query Frame = 0


Query: 1   MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI 60
           MEKT   S  APRSV + +EVWS+NLH+ELE+LNEY+PEF +V ID     IG       
Sbjct: 1   MEKTIRNSMPAPRSVRKKQEVWSHNLHEELELLNEYIPEFLVVTID-----IG------- 60

Query: 61  YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN 120
                   +++ RK+      LR +P               + RLNILQLGLTL +Q  N
Sbjct: 61  --------WYFGRKI------LRLSP---------------IQRLNILQLGLTLVDQKEN 120

Query: 121 VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR 180
           VAMT EFNFRDFDHISDM+G+S LD FLKN GFDFYKLK+DGI  E+FTTLFLPIC SGR
Sbjct: 121 VAMTLEFNFRDFDHISDMKGISLLDLFLKNNGFDFYKLKKDGIVPENFTTLFLPICHSGR 180

Query: 181 IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 240
           IER ITFHGFY+IAYLLK LKIK+IPI MATFAATAQHLLG +S+LKHM RYCDGLLDG+
Sbjct: 181 IERLITFHGFYEIAYLLKRLKIKFIPIFMATFAATAQHLLGAISNLKHMARYCDGLLDGN 240

Query: 241 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAF 300
           L LKK AKLLDVKRIG AHFAG DSLLTA++YTKMKQ+FK+P+EQ EGFLY LPHRI+AF
Sbjct: 241 LALKKFAKLLDVKRIGTAHFAGFDSLLTASIYTKMKQMFKLPLEQCEGFLYSLPHRIKAF 274

Query: 301 RPIVKIVDPSAMTPP 315
             I+KI++PSA TPP
Sbjct: 301 WSILKIINPSATTPP 274

[Close]

BLAST of MC06g_new0442 vs. NCBI nr
Match: XP_022928830.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 362 bits (929), Expect = 1.06e-121
Identity = 183/295 (62.03%), Postives = 217/295 (73.56%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           REVWS+NL +E+E++  YL EFPIVAIDTEFPG                           
Sbjct: 17  REVWSHNLLEEIELMKHYLSEFPIVAIDTEFPG--------------------------- 76

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVAMTWEFNFRDFDHISDM 138
             FLR+TPKGVS+EEFY CL+FNVNRL+ILQLGLTL + NG + MTWEFNF+DFD IS+M
Sbjct: 77  --FLRWTPKGVSDEEFYQCLQFNVNRLSILQLGLTLTDDNGKIGMTWEFNFQDFDQISNM 136

Query: 139 EGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRIERWITFHGFYDIAYLLK 198
           EGV SLDSFLKNKGF+F KL+  GIA ++F+  F P+ RSGRI++WITFHG YDIAYL+K
Sbjct: 137 EGVYSLDSFLKNKGFNFNKLRSCGIAPQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMK 196

Query: 199 LLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKLLDVKRIGIA 258
              I  +P SM+ FA  A HLLGTV+DLKHM RY D L +G+LGLKKLAKLL + R+G A
Sbjct: 197 FNNIISMPTSMSMFAMIAAHLLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTA 256

Query: 259 HFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAFRPIVKIVDPSAMT 313
           HFAGSDSLLTA+VY KMK I KV  +  EGFLYGLP+ IQ +RP V IVDPS +T
Sbjct: 257 HFAGSDSLLTASVYAKMKVIMKVEPKIHEGFLYGLPYPIQGYRPPVVIVDPSKLT 282

[Close]

BLAST of MC06g_new0442 vs. NCBI nr
Match: XP_023550519.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 359 bits (922), Expect = 1.14e-120
Identity = 184/295 (62.37%), Postives = 216/295 (73.22%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           REVWSYNL +E+E++  YL EFPIVA+DTEFPG                           
Sbjct: 15  REVWSYNLLEEIELMKHYLSEFPIVAMDTEFPG--------------------------- 74

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVAMTWEFNFRDFDHISDM 138
             FLR+TPKGVS+EEFY CL+FNVNRL+ILQLGLTL ++NG + MTWEFNF+DFD IS+M
Sbjct: 75  --FLRWTPKGVSDEEFYQCLQFNVNRLSILQLGLTLIDENGKIGMTWEFNFKDFDKISNM 134

Query: 139 EGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRIERWITFHGFYDIAYLLK 198
           EGV SLDSFLKNKGF+F KL+  GIA E+F+  F P+ RS RI+RWITFHG YDIAYL+K
Sbjct: 135 EGVYSLDSFLKNKGFNFNKLRSCGIAPEEFSRQFNPVIRSRRIQRWITFHGLYDIAYLMK 194

Query: 199 LLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKLLDVKRIGIA 258
              I  +P SM+ FA  A HLLGTV+DLKHM RY D L +G+LGLKKLAKLL + RIG A
Sbjct: 195 FNNIISMPTSMSMFAIIAAHLLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRIGTA 254

Query: 259 HFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAFRPIVKIVDPSAMT 313
           HFAGSDSLLTA+VY KMK I KV  +  EGFLYGLP+ IQ +R  V IVDPS +T
Sbjct: 255 HFAGSDSLLTASVYAKMKAIMKVEPKIHEGFLYGLPYPIQGYRRPVVIVDPSKLT 280

[Close]

BLAST of MC06g_new0442 vs. ExPASy TrEMBL
Match: A0A6J1DLA5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=3 SV=1)

HSP 1 Score: 707 bits (1824), Expect = 2.41e-257
Identity = 344/347 (99.14%), Postives = 346/347 (99.71%), Query Frame = 0


Query: 1   MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI 60
           MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI
Sbjct: 1   MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI 60

Query: 61  YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN 120
           YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN
Sbjct: 61  YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN 120

Query: 121 VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR 180
           VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR
Sbjct: 121 VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR 180

Query: 181 IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 240
           IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD
Sbjct: 181 IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 240

Query: 241 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAF 300
           LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQ+FKVPVEQREGFLYGLPHRIQAF
Sbjct: 241 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQMFKVPVEQREGFLYGLPHRIQAF 300

Query: 301 RPIVKIVDPSAMTPPPPCLPTLCFGSFPPFFIDIHSSKLKGHSVTVS 347
           +PIVKIVDPSAMTPPPP LPTLCFGSFPPFFIDIHSSKLKGHSVTVS
Sbjct: 301 QPIVKIVDPSAMTPPPPRLPTLCFGSFPPFFIDIHSSKLKGHSVTVS 347

[Close]

BLAST of MC06g_new0442 vs. ExPASy TrEMBL
Match: A0A6J1CXZ5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=3 SV=1)

HSP 1 Score: 477 bits (1228), Expect = 1.13e-166
Identity = 249/351 (70.94%), Postives = 271/351 (77.21%), Query Frame = 0


Query: 1   MEKTF-GKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNR 60
           MEK    KS  APRS+  NREVWS NL +ELE+LNEYLPEFP+V ID EFPG        
Sbjct: 19  MEKAIVRKSTAAPRSICHNREVWSLNLREELELLNEYLPEFPVVVIDMEFPG-------- 78

Query: 61  IYFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNG 120
                                FL  TPKG+SEEEFY CLRFNVNRLNILQLGLTLA+Q G
Sbjct: 79  ---------------------FLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKG 138

Query: 121 NVAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSG 180
           N+AMTWEFNFRDFDH+SDME VSSLDSFLKNKGFDFYKLK+ GIA EDFTTLFLPICRSG
Sbjct: 139 NIAMTWEFNFRDFDHVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSG 198

Query: 181 RIERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDG 240
           +I+RWITFHGFYDI YLLKLLKIK IPISMATFA TAQHLLGTVSDLKHM RYCDGLLDG
Sbjct: 199 QIKRWITFHGFYDITYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMARYCDGLLDG 258

Query: 241 DLGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQA 300
           +LGLKKLAKLLDVKRI  AHFA SD+L T +VYTKMKQIFK+P+EQ EGFLYGLP+ I  
Sbjct: 259 NLGLKKLAKLLDVKRIDNAHFACSDNLFTTSVYTKMKQIFKLPLEQCEGFLYGLPYPING 318

Query: 301 FRPIVKIVDPSAMTPPPPCLPT----LCFGSFPPFFIDIHSSKLKGHSVTV 346
           F+ + KI+DPS MTPPPP LP+    L F S  P  + I SSKL G SV V
Sbjct: 319 FQSVAKIIDPSTMTPPPP-LPSKFSILSFCSSTPLLVGIKSSKLNGPSVIV 339

[Close]

BLAST of MC06g_new0442 vs. ExPASy TrEMBL
Match: A0A6J1DIB1 (putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LOC111021342 PE=4 SV=1)

HSP 1 Score: 393 bits (1009), Expect = 4.34e-134
Identity = 206/315 (65.40%), Postives = 238/315 (75.56%), Query Frame = 0


Query: 1   MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI 60
           MEKT   S  APRSV + +EVWS+NLH+ELE+LNEY+PEF +V ID     IG       
Sbjct: 1   MEKTIRNSMPAPRSVRKKQEVWSHNLHEELELLNEYIPEFLVVTID-----IG------- 60

Query: 61  YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN 120
                   +++ RK+      LR +P               + RLNILQLGLTL +Q  N
Sbjct: 61  --------WYFGRKI------LRLSP---------------IQRLNILQLGLTLVDQKEN 120

Query: 121 VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR 180
           VAMT EFNFRDFDHISDM+G+S LD FLKN GFDFYKLK+DGI  E+FTTLFLPIC SGR
Sbjct: 121 VAMTLEFNFRDFDHISDMKGISLLDLFLKNNGFDFYKLKKDGIVPENFTTLFLPICHSGR 180

Query: 181 IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 240
           IER ITFHGFY+IAYLLK LKIK+IPI MATFAATAQHLLG +S+LKHM RYCDGLLDG+
Sbjct: 181 IERLITFHGFYEIAYLLKRLKIKFIPIFMATFAATAQHLLGAISNLKHMARYCDGLLDGN 240

Query: 241 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAF 300
           L LKK AKLLDVKRIG AHFAG DSLLTA++YTKMKQ+FK+P+EQ EGFLY LPHRI+AF
Sbjct: 241 LALKKFAKLLDVKRIGTAHFAGFDSLLTASIYTKMKQMFKLPLEQCEGFLYSLPHRIKAF 274

Query: 301 RPIVKIVDPSAMTPP 315
             I+KI++PSA TPP
Sbjct: 301 WSILKIINPSATTPP 274

[Close]

BLAST of MC06g_new0442 vs. ExPASy TrEMBL
Match: A0A6J1ESM7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3 SV=1)

HSP 1 Score: 362 bits (929), Expect = 5.13e-122
Identity = 183/295 (62.03%), Postives = 217/295 (73.56%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           REVWS+NL +E+E++  YL EFPIVAIDTEFPG                           
Sbjct: 17  REVWSHNLLEEIELMKHYLSEFPIVAIDTEFPG--------------------------- 76

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVAMTWEFNFRDFDHISDM 138
             FLR+TPKGVS+EEFY CL+FNVNRL+ILQLGLTL + NG + MTWEFNF+DFD IS+M
Sbjct: 77  --FLRWTPKGVSDEEFYQCLQFNVNRLSILQLGLTLTDDNGKIGMTWEFNFQDFDQISNM 136

Query: 139 EGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRIERWITFHGFYDIAYLLK 198
           EGV SLDSFLKNKGF+F KL+  GIA ++F+  F P+ RSGRI++WITFHG YDIAYL+K
Sbjct: 137 EGVYSLDSFLKNKGFNFNKLRSCGIAPQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMK 196

Query: 199 LLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKLLDVKRIGIA 258
              I  +P SM+ FA  A HLLGTV+DLKHM RY D L +G+LGLKKLAKLL + R+G A
Sbjct: 197 FNNIISMPTSMSMFAMIAAHLLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTA 256

Query: 259 HFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAFRPIVKIVDPSAMT 313
           HFAGSDSLLTA+VY KMK I KV  +  EGFLYGLP+ IQ +RP V IVDPS +T
Sbjct: 257 HFAGSDSLLTASVYAKMKVIMKVEPKIHEGFLYGLPYPIQGYRPPVVIVDPSKLT 282

[Close]

BLAST of MC06g_new0442 vs. ExPASy TrEMBL
Match: A0A6J1HL76 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3 SV=1)

HSP 1 Score: 356 bits (914), Expect = 9.06e-120
Identity = 186/295 (63.05%), Postives = 214/295 (72.54%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           R+VWS+NL +E+E++ + L EFPIVAIDTEFPG                           
Sbjct: 17  RDVWSHNLLEEIELIKQCLSEFPIVAIDTEFPG--------------------------- 76

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVAMTWEFNFRDFDHISDM 138
             FLR+TPKGVS+EEFY+CL+FNVNRL+ILQLGLTL N NG + MTWEFNFRDFD IS+M
Sbjct: 77  --FLRWTPKGVSDEEFYECLQFNVNRLSILQLGLTLTNDNGKIGMTWEFNFRDFDKISNM 136

Query: 139 EGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRIERWITFHGFYDIAYLLK 198
           EGV SLDSFLKNKGF+F  L+  GIA E+F T F PI RSGRI RWITFHG YDIAYL+K
Sbjct: 137 EGVYSLDSFLKNKGFNFNTLRSYGIAPEEFGTQFSPILRSGRIHRWITFHGLYDIAYLMK 196

Query: 199 LLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKLLDVKRIGIA 258
              I+ +P SM+ FA TA H+LGTV+DLKHM  Y   LLDG+LGLKKLAKLL V R G A
Sbjct: 197 FTNIRCMPTSMSIFAITAAHILGTVTDLKHMAWY--SLLDGNLGLKKLAKLLQVNRTGTA 256

Query: 259 HFAGSDSLLTAAVYTKMKQIFKVPVEQREGFLYGLPHRIQAFRPIVKIVDPSAMT 313
           HFAGSDSLLTA+VY KMK I KV     EGFLYGLP+ IQ +R  V IVDPS +T
Sbjct: 257 HFAGSDSLLTASVYAKMKIIMKVEPNIHEGFLYGLPYPIQGYRRPVVIVDPSKLT 280

[Close]

BLAST of MC06g_new0442 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.8e-44
Identity = 111/294 (37.76%), Postives = 155/294 (52.72%), Query Frame = 0


Query: 17  QNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGI-----GLLKDNRIYFLSLAGKYFY 76
           Q REVW+ NL +E++++ + + +FP VA+DTEFPGI     G  K N  Y          
Sbjct: 11  QIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY---------- 70

Query: 77  ARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVA-------MT 136
                                  Y+ L+ NVN L ++QLGLT +N+ GN+          
Sbjct: 71  ----------------------HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCI 130

Query: 137 WEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRI--- 196
           W+FNFR+FD  SD+  + S++  LK  G D  K   DGI  + F  L +    SG +   
Sbjct: 131 WQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKNTLDGIDSKRFAELLM---SSGIVLNE 190

Query: 197 -ERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 256
              W+TFH  YD  YLLKLL  + +P S   F         TV D+KH+ ++C+ L    
Sbjct: 191 NVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLINVYFPTVYDIKHLMKFCNSLHG-- 250

Query: 257 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKV-PVEQREGFLYGL 294
            GL KLA+LL+V+R+GI H AGSDSLLT+  + K+K+ F V P+ +  G LYGL
Sbjct: 251 -GLNKLAELLEVERVGICHQAGSDSLLTSCTFRKLKENFFVGPLHKYSGVLYGL 265

[Close]

BLAST of MC06g_new0442 vs. TAIR 10
Match: AT1G80780.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.8e-44
Identity = 111/294 (37.76%), Postives = 155/294 (52.72%), Query Frame = 0


Query: 17  QNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGI-----GLLKDNRIYFLSLAGKYFY 76
           Q REVW+ NL +E++++ + + +FP VA+DTEFPGI     G  K N  Y          
Sbjct: 11  QIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY---------- 70

Query: 77  ARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVA-------MT 136
                                  Y+ L+ NVN L ++QLGLT +N+ GN+          
Sbjct: 71  ----------------------HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCI 130

Query: 137 WEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRI--- 196
           W+FNFR+FD  SD+  + S++  LK  G D  K   DGI  + F  L +    SG +   
Sbjct: 131 WQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKNTLDGIDSKRFAELLM---SSGIVLNE 190

Query: 197 -ERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 256
              W+TFH  YD  YLLKLL  + +P S   F         TV D+KH+ ++C+ L    
Sbjct: 191 NVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLINVYFPTVYDIKHLMKFCNSLHG-- 250

Query: 257 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKV-PVEQREGFLYGL 294
            GL KLA+LL+V+R+GI H AGSDSLLT+  + K+K+ F V P+ +  G LYGL
Sbjct: 251 -GLNKLAELLEVERVGICHQAGSDSLLTSCTFRKLKENFFVGPLHKYSGVLYGL 265

[Close]

BLAST of MC06g_new0442 vs. TAIR 10
Match: AT1G80780.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.8e-44
Identity = 111/294 (37.76%), Postives = 155/294 (52.72%), Query Frame = 0


Query: 17  QNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGI-----GLLKDNRIYFLSLAGKYFY 76
           Q REVW+ NL +E++++ + + +FP VA+DTEFPGI     G  K N  Y          
Sbjct: 11  QIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRPVGTFKSNADY---------- 70

Query: 77  ARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNVA-------MT 136
                                  Y+ L+ NVN L ++QLGLT +N+ GN+          
Sbjct: 71  ----------------------HYETLKTNVNILKMIQLGLTFSNEQGNLPTCGTDKYCI 130

Query: 137 WEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGRI--- 196
           W+FNFR+FD  SD+  + S++  LK  G D  K   DGI  + F  L +    SG +   
Sbjct: 131 WQFNFREFDLDSDIFALDSIE-LLKQSGIDLAKNTLDGIDSKRFAELLM---SSGIVLNE 190

Query: 197 -ERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 256
              W+TFH  YD  YLLKLL  + +P S   F         TV D+KH+ ++C+ L    
Sbjct: 191 NVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFKLINVYFPTVYDIKHLMKFCNSLHG-- 250

Query: 257 LGLKKLAKLLDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKV-PVEQREGFLYGL 294
            GL KLA+LL+V+R+GI H AGSDSLLT+  + K+K+ F V P+ +  G LYGL
Sbjct: 251 -GLNKLAELLEVERVGICHQAGSDSLLTSCTFRKLKENFFVGPLHKYSGVLYGL 265

[Close]

BLAST of MC06g_new0442 vs. TAIR 10
Match: AT1G15920.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 103/285 (36.14%), Postives = 155/285 (54.39%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           REVW++NL +E+ ++ + + +FP VA+DTEFPGI                    + +   
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGI------------------VCKTVTAN 76

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNV-------AMTWEFNFRD 138
           P      P  +  E  YD L+ NVN L ++QLGLTL+++ GN+          W+FNFR+
Sbjct: 77  P---NPNPYSIHYEYNYDTLKANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFRE 136

Query: 139 FDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFL--PICRSGRIERWITFHG 198
           F+ ISDM  + S++  L+    D  K    G+  + F  L +   +  + +I  W+TFH 
Sbjct: 137 FNVISDMFALDSIE-LLRKSAIDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHC 196

Query: 199 FYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKL 258
            YD  YLLKLL  K +P  ++ F    +     V D+K++  +C  L     GL+K+A+L
Sbjct: 197 GYDFGYLLKLLSGKELPEEISDFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAEL 256

Query: 259 LDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKV-PVEQREGFLYGL 294
           L VKR+GI+H AGSDSLLT   + KMK+ F    + +  GFL+GL
Sbjct: 257 LGVKRVGISHQAGSDSLLTLRTFIKMKEFFFTGSLLKYSGFLFGL 275

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BLAST of MC06g_new0442 vs. TAIR 10
Match: AT1G15920.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 103/285 (36.14%), Postives = 155/285 (54.39%), Query Frame = 0


Query: 19  REVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRIYFLSLAGKYFYARKLDDE 78
           REVW++NL +E+ ++ + + +FP VA+DTEFPGI                    + +   
Sbjct: 17  REVWNHNLEQEMALIEQSIDDFPYVAMDTEFPGI------------------VCKTVTAN 76

Query: 79  PGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGNV-------AMTWEFNFRD 138
           P      P  +  E  YD L+ NVN L ++QLGLTL+++ GN+          W+FNFR+
Sbjct: 77  P---NPNPYSIHYEYNYDTLKANVNMLKLIQLGLTLSDEKGNLPTCGTNKQCIWQFNFRE 136

Query: 139 FDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFL--PICRSGRIERWITFHG 198
           F+ ISDM  + S++  L+    D  K    G+  + F  L +   +  + +I  W+TFH 
Sbjct: 137 FNVISDMFALDSIE-LLRKSAIDLEKNNECGVDAKRFAELLMGSGVVLNDKI-HWVTFHC 196

Query: 199 FYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGDLGLKKLAKL 258
            YD  YLLKLL  K +P  ++ F    +     V D+K++  +C  L     GL+K+A+L
Sbjct: 197 GYDFGYLLKLLSGKELPEEISDFFDQMEKFFPVVYDIKYLMGFCTNLYG---GLEKIAEL 256

Query: 259 LDVKRIGIAHFAGSDSLLTAAVYTKMKQIFKV-PVEQREGFLYGL 294
           L VKR+GI+H AGSDSLLT   + KMK+ F    + +  GFL+GL
Sbjct: 257 LGVKRVGISHQAGSDSLLTLRTFIKMKEFFFTGSLLKYSGFLFGL 275

The following BLAST results are available for this feature:

BLAST of MC06g_new0442 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAI2	2.5e-43	37.76	Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9S9P2	2.3e-41	36.14	Probable CCR4-associated factor 1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SKZ2	1.2e-40	36.27	Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LXM2	5.8e-40	34.71	Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9LEU4	2.4e-38	35.79	Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of MC06g_new0442 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154798.1	4.99e-257	99.14	probable CCR4-associated factor 1 homolog 6 [Momordica charantia]	[more]
XP_022146384.1	2.33e-166	70.94	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022153955.1	8.96e-134	65.40	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]
XP_022928830.1	1.06e-121	62.03	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
XP_023550519.1	1.14e-120	62.37	putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC06g_new0442 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLA5	2.41e-257	99.14	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=...	[more]
A0A6J1CXZ5	1.13e-166	70.94	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=...	[more]
A0A6J1DIB1	4.34e-134	65.40	putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1ESM7	5.13e-122	62.03	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3...	[more]
A0A6J1HL76	9.06e-120	63.05	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111463977 PE=3...	[more]

BLAST of MC06g_new0442 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80780.1	1.8e-44	37.76	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.2	1.8e-44	37.76	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G80780.3	1.8e-44	37.76	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G15920.2	1.7e-42	36.14	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G15920.1	1.7e-42	36.14	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 21..163 e-value: 7.4E-6 score: 25.5
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 7..296 e-value: 1.8E-70 score: 239.1
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 77..301 coord: 18..52
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 77..301 coord: 18..52
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 12..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g_new0442.1	MC06g_new0442.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006402	mRNA catabolic process
biological_process	GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component	GO:0030014	CCR4-NOT complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity