MC06g_new0096 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0096
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
LocationMC06: 3660308 .. 3662009 (+)
RNA-Seq ExpressionMC06g_new0096
SyntenyMC06g_new0096
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATTAGTAAGATAAGAGAAATATGTAAATAAACAAGGGAAGGACAAGATACCTAAGCCTTTTAAGCGAAATTAAAAGCGATGGAATTTGGAGTAATAGAGATAAAGTGAATGAGATGGAAAGGGAATTTGGTTAAAAAGAATTGAGAAGAGGCCGAGGGTTGTGTGTGCATAGTGAAGATGGTATGGTGTCACACATTCAAAATCCCATGCACATTATATTGCCATCTGTGTCCACCTCTGCATTCCCAACCGCCTCCCCTCTCTGTTTTCCCCTTATATAATCCCGCCTCTCCCCCAACTCCAACTCCAACTCCAAACATACCAATAATTATTGAGCCCCAACAACCTCAAATATCCAATGCCTCCTCCTGCTTTGTCACTCCTGCTCTTTCTGACTCTCTCTGTTTCCTCTGTTTTTGCTGCCAACTTCAACCAGGATGTTGAGCTAATTTGGGGCAATGAACGTGCCAATATCCTTGACGATGGCCACCTCCTCACTCTCTCCTTGGACCGAGCCTCTGGCTCCGGCTTCCAGTCCAAAAATGTTTACCTTTTTGGCAAAATTGAGATGCAGCTCAAACTTGTCCCTGGAAACTCCGCTGGCACTGTCACCACTTACTATGTATGAATATAAACTGATTTACTTTATCCAACCCAACCCAACCCAATTGCCTTGCACACACATGTTTCTGATTTAATATATATATATATATATATATGTATGTATGTATGTATATTTGTTTTGTATGCAGCTTAAATCCGAAGGGTCGACGTGGGATGAGATAGATTTTGAATTCTTGGGGAATTTGACTGGTGACCCTTACACTCTTCACACCAATGTTTTTACCAATGGCGTGGGCAAGAGAGAGCAGCAATTCCACCTCTGGTTTGATCCCACTACCGATTTCCACACCTACTCAATCCTCTGGAACCCGCAGCGGATCATGTATGTCTTATCAACCAATCATGTTTTTAAATTTGAAAATTTTATTTTGTTAATTTCGGTTAAATTGGGGTTTGTTTCAGCTTCAAGGTGGATGGCACGCCCGTCAGGGAGTTCAAGAACGAGGAATCCTCGGGCGTTATGTTCCCAACGAATCAGCCGATGAGGCTCTACTCCAGCCTCTGGAACGCCGACGACTGGGCCACCAGAGGAGGGCTCGTCAAGACGGATTGGTCCAAAGCTCCCTTCACCGCCGCCTACCGGAACTTCAACGCCGACTCCTGCGTTTGGTCCGCCGGCGGCTCCTCCTGCTCCTCGGGCGCCCCCTCTGGCCACGGATGGCTCTCCGAAAACCTGGACAACACTCAACAACAGAGGCTGAGGTGGGTGCAGAAAAATTTCATGATTTACAATTACTGCACGGACGCCAAGAGGTTCCCGCAAGGCTTCCCTCCCGAGTGCTCTGTCAACGCCGGATCCTAAATTTGCCCCACGTCGCGTTCCCTAATTTGCTTCCGCCCAAAGTACAAATTATACAATATAAAGTATATTGTATGTATAGAAGAAGATCAGATCATACATGCGTAATCTTGCTTTTCATGCCTCATGTACTCTGTTTTTCTGTTGCTCCTTCATCTTCCAAATCTATTTGGAAAAATCATTAATAATAATTGTTAGTTTTTTTTTTTCTTTTACATTTTTGCTACGGGAATTAATAAAAAATTTCCGGAACAGGAACATAGGATATGAAACTGGC

mRNA sequence

ATGCCTCCTCCTGCTTTGTCACTCCTGCTCTTTCTGACTCTCTCTGTTTCCTCTGTTTTTGCTGCCAACTTCAACCAGGATGTTGAGCTAATTTGGGGCAATGAACGTGCCAATATCCTTGACGATGGCCACCTCCTCACTCTCTCCTTGGACCGAGCCTCTGGCTCCGGCTTCCAGTCCAAAAATGTTTACCTTTTTGGCAAAATTGAGATGCAGCTCAAACTTGTCCCTGGAAACTCCGCTGGCACTGTCACCACTTACTATCTTAAATCCGAAGGGTCGACGTGGGATGAGATAGATTTTGAATTCTTGGGGAATTTGACTGGTGACCCTTACACTCTTCACACCAATGTTTTTACCAATGGCGTGGGCAAGAGAGAGCAGCAATTCCACCTCTGGTTTGATCCCACTACCGATTTCCACACCTACTCAATCCTCTGGAACCCGCAGCGGATCATCTTCAAGGTGGATGGCACGCCCGTCAGGGAGTTCAAGAACGAGGAATCCTCGGGCGTTATGTTCCCAACGAATCAGCCGATGAGGCTCTACTCCAGCCTCTGGAACGCCGACGACTGGGCCACCAGAGGAGGGCTCGTCAAGACGGATTGGTCCAAAGCTCCCTTCACCGCCGCCTACCGGAACTTCAACGCCGACTCCTGCGTTTGGTCCGCCGGCGGCTCCTCCTGCTCCTCGGGCGCCCCCTCTGGCCACGGATGGCTCTCCGAAAACCTGGACAACACTCAACAACAGAGGCTGAGGTGGGTGCAGAAAAATTTCATGATTTACAATTACTGCACGGACGCCAAGAGGTTCCCGCAAGGCTTCCCTCCCGAGTGCTCTGTCAACGCCGGATCCTAA

Coding sequence (CDS)

ATGCCTCCTCCTGCTTTGTCACTCCTGCTCTTTCTGACTCTCTCTGTTTCCTCTGTTTTTGCTGCCAACTTCAACCAGGATGTTGAGCTAATTTGGGGCAATGAACGTGCCAATATCCTTGACGATGGCCACCTCCTCACTCTCTCCTTGGACCGAGCCTCTGGCTCCGGCTTCCAGTCCAAAAATGTTTACCTTTTTGGCAAAATTGAGATGCAGCTCAAACTTGTCCCTGGAAACTCCGCTGGCACTGTCACCACTTACTATCTTAAATCCGAAGGGTCGACGTGGGATGAGATAGATTTTGAATTCTTGGGGAATTTGACTGGTGACCCTTACACTCTTCACACCAATGTTTTTACCAATGGCGTGGGCAAGAGAGAGCAGCAATTCCACCTCTGGTTTGATCCCACTACCGATTTCCACACCTACTCAATCCTCTGGAACCCGCAGCGGATCATCTTCAAGGTGGATGGCACGCCCGTCAGGGAGTTCAAGAACGAGGAATCCTCGGGCGTTATGTTCCCAACGAATCAGCCGATGAGGCTCTACTCCAGCCTCTGGAACGCCGACGACTGGGCCACCAGAGGAGGGCTCGTCAAGACGGATTGGTCCAAAGCTCCCTTCACCGCCGCCTACCGGAACTTCAACGCCGACTCCTGCGTTTGGTCCGCCGGCGGCTCCTCCTGCTCCTCGGGCGCCCCCTCTGGCCACGGATGGCTCTCCGAAAACCTGGACAACACTCAACAACAGAGGCTGAGGTGGGTGCAGAAAAATTTCATGATTTACAATTACTGCACGGACGCCAAGAGGTTCCCGCAAGGCTTCCCTCCCGAGTGCTCTGTCAACGCCGGATCCTAA

Protein sequence

MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWLSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS
Homology
BLAST of MC06g_new0096 vs. ExPASy Swiss-Prot
Match: Q38857 (Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=3702 GN=XTH22 PE=1 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 6.0e-120
Identity = 197/275 (71.64%), Postives = 231/275 (84.00%), Query Frame = 0

Query: 8   LLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFG 67
           L LFL+L ++S  +ANF +DVE+ WG+ R  I ++G LLTLSLD++SGSGFQSKN YLFG
Sbjct: 7   LPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSKNEYLFG 66

Query: 68  KIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKRE 127
           K+ MQ+KLVPGNSAGTVTT YLKS G+TWDEIDFEFLGN +G+PYTLHTNV+T G G +E
Sbjct: 67  KVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQGKGDKE 126

Query: 128 QQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLW 187
           QQF LWFDPT +FHTY+ILWNPQRIIF VDGTP+REFKN ES G +FP N+PMR+YSSLW
Sbjct: 127 QQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRMYSSLW 186

Query: 188 NADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSG---HGWLSENL 247
           NADDWATRGGLVKTDWSKAPFTA+YR F  ++CVWS G SSC + +  G     WLS+ L
Sbjct: 187 NADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSCPNASKQGTTTGSWLSQEL 246

Query: 248 DNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 280
           D+T QQR+RWVQ+N+MIYNYCTDAKRFPQG P EC
Sbjct: 247 DSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MC06g_new0096 vs. ExPASy Swiss-Prot
Match: Q38910 (Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis thaliana OX=3702 GN=XTH23 PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 1.9e-118
Identity = 202/274 (73.72%), Postives = 227/274 (82.85%), Query Frame = 0

Query: 8   LLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFG 67
           L  F+  SVS    ANF +DVE+ WG+ R  I ++G LLTLSLD+ASGSGFQSKN YLFG
Sbjct: 14  LASFMICSVS----ANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYLFG 73

Query: 68  KIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKRE 127
           KI+MQ+KLV GNSAGTVT YYLKS GSTWDEIDFEFLGNL+GDPYTLHTNVFT G G RE
Sbjct: 74  KIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDRE 133

Query: 128 QQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLW 187
           QQF LWFDPT+DFHTYSILWNPQRIIF VDGTP+REFKN ES G +FP NQPMR+YSSLW
Sbjct: 134 QQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSLW 193

Query: 188 NADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCS--SGAPSGHGWLSENLD 247
           NA++WATRGGLVKTDWSKAPFTA+YR FN ++CV   G SSC   SG  S   WLS+ LD
Sbjct: 194 NAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQELD 253

Query: 248 NTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 280
           +T Q+++RWVQ N+MIYNYCTDAKRFPQG P EC
Sbjct: 254 STGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MC06g_new0096 vs. ExPASy Swiss-Prot
Match: Q38907 (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana OX=3702 GN=XTH25 PE=2 SV=2)

HSP 1 Score: 416.0 bits (1068), Expect = 3.4e-115
Identity = 194/278 (69.78%), Postives = 223/278 (80.22%), Query Frame = 0

Query: 6   LSLLLFLTLSV----SSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSK 65
           LSLL  LT+S     S VFA  F+ + ++ WG+ R  +L++G LLTLSLDRASGSGFQ+K
Sbjct: 8   LSLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTK 67

Query: 66  NVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTN 125
             YLFGKI+MQLKLVPGNSAGTVT YYLKS+G TWDEIDFEFLGNLTGDPYT+HTNV+T 
Sbjct: 68  KEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQ 127

Query: 126 GVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMR 185
           G G REQQFHLWFDPT DFHTYS+LWNP  I+F VD  PVREFKN +  G+ +P  QPMR
Sbjct: 128 GKGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMR 187

Query: 186 LYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWLS 245
           LYSSLWNAD WATRGGLVKTDWSKAPFTA+YRNF AD+CV S G SSC +G+P    W S
Sbjct: 188 LYSSLWNADQWATRGGLVKTDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPR---WFS 247

Query: 246 ENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 280
           + LD T + ++R VQ+ +MIYNYCTD KRFPQGFP EC
Sbjct: 248 QRLDLTAEDKMRVVQRKYMIYNYCTDTKRFPQGFPKEC 282

BLAST of MC06g_new0096 vs. ExPASy Swiss-Prot
Match: P24806 (Xyloglucan endotransglucosylase/hydrolase protein 24 OS=Arabidopsis thaliana OX=3702 GN=XTH24 PE=1 SV=2)

HSP 1 Score: 406.8 bits (1044), Expect = 2.1e-112
Identity = 188/283 (66.43%), Postives = 221/283 (78.09%), Query Frame = 0

Query: 1   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 60
           M P  +     L ++  SV AA+FN DV + WGN R  IL++G LLTLSLD++SGSGFQS
Sbjct: 1   MSPFKIFFFTTLLVAAFSVSAADFNTDVNVAWGNGRGKILNNGQLLTLSLDKSSGSGFQS 60

Query: 61  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 120
           K  YLFGKI+MQ+KLVPGNSAGTVTT+YLKSEGSTWDEIDFEFLGN++GDPYTLHTNV+T
Sbjct: 61  KTEYLFGKIDMQIKLVPGNSAGTVTTFYLKSEGSTWDEIDFEFLGNMSGDPYTLHTNVYT 120

Query: 121 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 180
            G G +EQQFHLWFDPT +FHTYSILWNPQRII  VD TP+REFKN ES GV+FP N+PM
Sbjct: 121 QGKGDKEQQFHLWFDPTANFHTYSILWNPQRIILTVDDTPIREFKNYESLGVLFPKNKPM 180

Query: 181 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL 240
           R+Y+SLWNADDWATRGGLVKTDWSKAPF A+YRN   DS                   W 
Sbjct: 181 RMYASLWNADDWATRGGLVKTDWSKAPFMASYRNIKIDS--------------KPNSNWY 240

Query: 241 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNA 284
           ++ +D+T Q RL+WVQKN+MIYNYCTD +RFPQG P EC+ ++
Sbjct: 241 TQEMDSTSQARLKWVQKNYMIYNYCTDHRRFPQGAPKECTTSS 269

BLAST of MC06g_new0096 vs. ExPASy Swiss-Prot
Match: Q9FI31 (Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=3702 GN=XTH20 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 3.7e-109
Identity = 176/277 (63.54%), Postives = 220/277 (79.42%), Query Frame = 0

Query: 5   ALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDD-GHLLTLSLDRASGSGFQSKNV 64
           A  ++         V+A +F++DV++ WG+ R  ILD+ G+LL+LSLD+ SGSGFQS   
Sbjct: 10  AFLIIFLFAAQYERVYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQE 69

Query: 65  YLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGV 124
           +L+GK+E+Q+KLVPGNSAGTVTT+YLKS G+TWDEIDFEFLGN++G PYTLHTNV+T G 
Sbjct: 70  FLYGKVEVQMKLVPGNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGT 129

Query: 125 GKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLY 184
           G +EQQFHLWFDPT DFHTY I+WNPQR+IF +DG P+REFKN E+ GV FP +QPMRLY
Sbjct: 130 GDKEQQFHLWFDPTVDFHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLY 189

Query: 185 SSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWLSEN 244
           +SLW A+ WATRGGL KTDWSKAPFTA YRN+N D+CVWS G SSCS+ +     W ++ 
Sbjct: 190 ASLWEAEHWATRGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANS----SWFTQV 249

Query: 245 LDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECS 281
           LD   + R++W Q+ +M+YNYCTD KRFPQG PPECS
Sbjct: 250 LDFKGKNRVKWAQRKYMVYNYCTDKKRFPQGAPPECS 282

BLAST of MC06g_new0096 vs. NCBI nr
Match: XP_022135083.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Momordica charantia])

HSP 1 Score: 599 bits (1544), Expect = 1.93e-216
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 1   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 60
           MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS
Sbjct: 1   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 60

Query: 61  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 120
           KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT
Sbjct: 61  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 120

Query: 121 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 180
           NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM
Sbjct: 121 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 180

Query: 181 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL 240
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL
Sbjct: 181 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL 240

Query: 241 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 285
           SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS
Sbjct: 241 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 285

BLAST of MC06g_new0096 vs. NCBI nr
Match: CCH26406.1 (xyloglucan endotransglycosylase [Cucumis sativus])

HSP 1 Score: 493 bits (1270), Expect = 1.21e-174
Identity = 233/282 (82.62%), Postives = 253/282 (89.72%), Query Frame = 0

Query: 2   PPPALSLLLFLTL-SVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 61
           PPP  SLLLFL+L SVSSVF  NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQS
Sbjct: 4   PPPFSSLLLFLSLLSVSSVFCGNFYNDFEISWGNDRAKILNNGDLLTLSLDRGSGSGFQS 63

Query: 62  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 121
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 64  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 123

Query: 122 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 181
            G G REQQFHLWFDPT DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP NQPM
Sbjct: 124 QGKGDREQQFHLWFDPTYDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKNQPM 183

Query: 182 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAP-SGHGW 241
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNA+ CVWS G SSCSSG    G GW
Sbjct: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNANVCVWSGGASSCSSGTNVGGRGW 243

Query: 242 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 281
           LSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPECS+
Sbjct: 244 LSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECSL 285

BLAST of MC06g_new0096 vs. NCBI nr
Match: XP_004146309.1 (xyloglucan endotransglucosylase/hydrolase protein 22 [Cucumis sativus] >KGN65439.1 hypothetical protein Csa_019769 [Cucumis sativus])

HSP 1 Score: 491 bits (1263), Expect = 1.41e-173
Identity = 232/282 (82.27%), Postives = 252/282 (89.36%), Query Frame = 0

Query: 2   PPPALSLLLFLTL-SVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 61
           P P  SLLLFL+L SVSSVF  NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQS
Sbjct: 4   PSPFSSLLLFLSLLSVSSVFCGNFYNDFEITWGNDRAKILNNGDLLTLSLDRGSGSGFQS 63

Query: 62  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 121
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 64  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 123

Query: 122 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 181
            G G REQQFHLWFDPT DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP NQPM
Sbjct: 124 QGKGDREQQFHLWFDPTYDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKNQPM 183

Query: 182 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAP-SGHGW 241
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNA+ CVWS G SSCSSG    G GW
Sbjct: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNANVCVWSGGASSCSSGTNVGGRGW 243

Query: 242 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 281
           LSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPECS+
Sbjct: 244 LSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECSL 285

BLAST of MC06g_new0096 vs. NCBI nr
Match: XP_038880531.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Benincasa hispida])

HSP 1 Score: 489 bits (1258), Expect = 7.58e-173
Identity = 231/282 (81.91%), Postives = 253/282 (89.72%), Query Frame = 0

Query: 2   PPPALSLLLFLTL-SVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 61
           PPP  SLL FL+L SVSSVF  NF QD E+ WGN RANIL++G LLTLSLDR SGSGFQS
Sbjct: 3   PPPFSSLLFFLSLFSVSSVFCGNFYQDFEITWGNGRANILNNGELLTLSLDRGSGSGFQS 62

Query: 62  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 121
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 63  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 122

Query: 122 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 181
            G G REQQFHLWFDPT+DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP  QPM
Sbjct: 123 QGKGDREQQFHLWFDPTSDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKKQPM 182

Query: 182 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAP-SGHGW 241
           RLYSSLWNADDWATRGGLVKTDWS APFTA+YRNFNAD CVWSAG SSCSS A   G GW
Sbjct: 183 RLYSSLWNADDWATRGGLVKTDWSHAPFTASYRNFNADVCVWSAGVSSCSSDANVGGKGW 242

Query: 242 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 281
           LSENLD T+QQR++WVQ+N+MIYNYC+DAKRFPQG+PPEC++
Sbjct: 243 LSENLDITRQQRMKWVQRNYMIYNYCSDAKRFPQGYPPECAL 284

BLAST of MC06g_new0096 vs. NCBI nr
Match: NP_001284442.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like precursor [Cucumis melo] >ABI94062.1 xyloglucan endotransglucosylase/hydrolase 2 [Cucumis melo])

HSP 1 Score: 488 bits (1256), Expect = 1.64e-172
Identity = 230/283 (81.27%), Postives = 255/283 (90.11%), Query Frame = 0

Query: 2   PPPALS-LLLFLTL-SVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQ 61
           PPP+ S LLLFL+L SVSSVF +NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQ
Sbjct: 3   PPPSFSSLLLFLSLLSVSSVFCSNFYNDFEITWGNDRAKILNNGDLLTLSLDRGSGSGFQ 62

Query: 62  SKNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVF 121
           SKNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+
Sbjct: 63  SKNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVY 122

Query: 122 TNGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQP 181
           T G G REQQFHLWFDPT+DFHTYSILWNPQ IIF VDGTP+R+FKN ESSGV+FP  QP
Sbjct: 123 TQGKGDREQQFHLWFDPTSDFHTYSILWNPQGIIFSVDGTPIRKFKNHESSGVLFPKKQP 182

Query: 182 MRLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAP-SGHG 241
           MRLYSSLWNADDWATRGGLVKTDWSKAPFTA+YRNFNAD CVWS G SSCSSG    G G
Sbjct: 183 MRLYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNADVCVWSGGVSSCSSGGNVGGRG 242

Query: 242 WLSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 281
           WLSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPEC++
Sbjct: 243 WLSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECAI 285

BLAST of MC06g_new0096 vs. ExPASy TrEMBL
Match: A0A6J1C3T7 (Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC111007155 PE=3 SV=1)

HSP 1 Score: 599 bits (1544), Expect = 9.33e-217
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 1   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 60
           MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS
Sbjct: 1   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 60

Query: 61  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 120
           KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT
Sbjct: 61  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 120

Query: 121 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 180
           NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM
Sbjct: 121 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 180

Query: 181 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL 240
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL
Sbjct: 181 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL 240

Query: 241 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 285
           SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS
Sbjct: 241 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 285

BLAST of MC06g_new0096 vs. ExPASy TrEMBL
Match: N0DX33 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth6 PE=3 SV=1)

HSP 1 Score: 493 bits (1270), Expect = 5.86e-175
Identity = 233/282 (82.62%), Postives = 253/282 (89.72%), Query Frame = 0

Query: 2   PPPALSLLLFLTL-SVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 61
           PPP  SLLLFL+L SVSSVF  NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQS
Sbjct: 4   PPPFSSLLLFLSLLSVSSVFCGNFYNDFEISWGNDRAKILNNGDLLTLSLDRGSGSGFQS 63

Query: 62  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 121
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 64  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 123

Query: 122 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 181
            G G REQQFHLWFDPT DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP NQPM
Sbjct: 124 QGKGDREQQFHLWFDPTYDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKNQPM 183

Query: 182 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAP-SGHGW 241
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNA+ CVWS G SSCSSG    G GW
Sbjct: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNANVCVWSGGASSCSSGTNVGGRGW 243

Query: 242 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 281
           LSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPECS+
Sbjct: 244 LSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECSL 285

BLAST of MC06g_new0096 vs. ExPASy TrEMBL
Match: A0A0A0LWT1 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G422480 PE=3 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 6.83e-174
Identity = 232/282 (82.27%), Postives = 252/282 (89.36%), Query Frame = 0

Query: 2   PPPALSLLLFLTL-SVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 61
           P P  SLLLFL+L SVSSVF  NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQS
Sbjct: 4   PSPFSSLLLFLSLLSVSSVFCGNFYNDFEITWGNDRAKILNNGDLLTLSLDRGSGSGFQS 63

Query: 62  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 121
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 64  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 123

Query: 122 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 181
            G G REQQFHLWFDPT DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP NQPM
Sbjct: 124 QGKGDREQQFHLWFDPTYDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKNQPM 183

Query: 182 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAP-SGHGW 241
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNA+ CVWS G SSCSSG    G GW
Sbjct: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNANVCVWSGGASSCSSGTNVGGRGW 243

Query: 242 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 281
           LSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPECS+
Sbjct: 244 LSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECSL 285

BLAST of MC06g_new0096 vs. ExPASy TrEMBL
Match: Q06BI5 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=XTH2 PE=2 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 7.96e-173
Identity = 230/283 (81.27%), Postives = 255/283 (90.11%), Query Frame = 0

Query: 2   PPPALS-LLLFLTL-SVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQ 61
           PPP+ S LLLFL+L SVSSVF +NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQ
Sbjct: 3   PPPSFSSLLLFLSLLSVSSVFCSNFYNDFEITWGNDRAKILNNGDLLTLSLDRGSGSGFQ 62

Query: 62  SKNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVF 121
           SKNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+
Sbjct: 63  SKNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVY 122

Query: 122 TNGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQP 181
           T G G REQQFHLWFDPT+DFHTYSILWNPQ IIF VDGTP+R+FKN ESSGV+FP  QP
Sbjct: 123 TQGKGDREQQFHLWFDPTSDFHTYSILWNPQGIIFSVDGTPIRKFKNHESSGVLFPKKQP 182

Query: 182 MRLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAP-SGHG 241
           MRLYSSLWNADDWATRGGLVKTDWSKAPFTA+YRNFNAD CVWS G SSCSSG    G G
Sbjct: 183 MRLYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNADVCVWSGGVSSCSSGGNVGGRG 242

Query: 242 WLSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 281
           WLSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPEC++
Sbjct: 243 WLSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECAI 285

BLAST of MC06g_new0096 vs. ExPASy TrEMBL
Match: A0A6J1KN18 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497152 PE=3 SV=1)

HSP 1 Score: 485 bits (1248), Expect = 1.47e-171
Identity = 228/280 (81.43%), Postives = 250/280 (89.29%), Query Frame = 0

Query: 7   SLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLF 66
           SLLLF    VSSVF  NF QD E+ WGN+RANILD+G LLTLSLDR SGSGF+S NVYLF
Sbjct: 15  SLLLF----VSSVFGGNFYQDFEITWGNDRANILDNGDLLTLSLDRDSGSGFRSNNVYLF 74

Query: 67  GKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKR 126
            KI+MQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNV+T G G R
Sbjct: 75  AKIDMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVYTQGKGDR 134

Query: 127 EQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSL 186
           EQQFHLWFDPT DFHTYSILWNP+ IIF VDGTP+REFKN+ESSGV+FPT QPMRLYSSL
Sbjct: 135 EQQFHLWFDPTADFHTYSILWNPEGIIFSVDGTPIREFKNQESSGVLFPTKQPMRLYSSL 194

Query: 187 WNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPS-GHGWLSENLD 246
           WNADDWATRGGLVKTDWS+APF ++YRNF AD CVWS G SSCSSGA S G GWLSENLD
Sbjct: 195 WNADDWATRGGLVKTDWSQAPFVSSYRNFKADVCVWSEGVSSCSSGAHSAGGGWLSENLD 254

Query: 247 NTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 285
           NT+QQRL+WVQ+N+MIYNYCTDA+RFPQG+PPEC++N  +
Sbjct: 255 NTRQQRLKWVQRNYMIYNYCTDAERFPQGYPPECTLNTAA 290

BLAST of MC06g_new0096 vs. TAIR 10
Match: AT5G57560.1 (Xyloglucan endotransglucosylase/hydrolase family protein )

HSP 1 Score: 431.8 bits (1109), Expect = 4.3e-121
Identity = 197/275 (71.64%), Postives = 231/275 (84.00%), Query Frame = 0

Query: 8   LLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFG 67
           L LFL+L ++S  +ANF +DVE+ WG+ R  I ++G LLTLSLD++SGSGFQSKN YLFG
Sbjct: 7   LPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSKNEYLFG 66

Query: 68  KIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKRE 127
           K+ MQ+KLVPGNSAGTVTT YLKS G+TWDEIDFEFLGN +G+PYTLHTNV+T G G +E
Sbjct: 67  KVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQGKGDKE 126

Query: 128 QQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLW 187
           QQF LWFDPT +FHTY+ILWNPQRIIF VDGTP+REFKN ES G +FP N+PMR+YSSLW
Sbjct: 127 QQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRMYSSLW 186

Query: 188 NADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSG---HGWLSENL 247
           NADDWATRGGLVKTDWSKAPFTA+YR F  ++CVWS G SSC + +  G     WLS+ L
Sbjct: 187 NADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSCPNASKQGTTTGSWLSQEL 246

Query: 248 DNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 280
           D+T QQR+RWVQ+N+MIYNYCTDAKRFPQG P EC
Sbjct: 247 DSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MC06g_new0096 vs. TAIR 10
Match: AT4G25810.1 (xyloglucan endotransglycosylase 6 )

HSP 1 Score: 426.8 bits (1096), Expect = 1.4e-119
Identity = 202/274 (73.72%), Postives = 227/274 (82.85%), Query Frame = 0

Query: 8   LLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFG 67
           L  F+  SVS    ANF +DVE+ WG+ R  I ++G LLTLSLD+ASGSGFQSKN YLFG
Sbjct: 14  LASFMICSVS----ANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYLFG 73

Query: 68  KIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKRE 127
           KI+MQ+KLV GNSAGTVT YYLKS GSTWDEIDFEFLGNL+GDPYTLHTNVFT G G RE
Sbjct: 74  KIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDRE 133

Query: 128 QQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLW 187
           QQF LWFDPT+DFHTYSILWNPQRIIF VDGTP+REFKN ES G +FP NQPMR+YSSLW
Sbjct: 134 QQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSLW 193

Query: 188 NADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCS--SGAPSGHGWLSENLD 247
           NA++WATRGGLVKTDWSKAPFTA+YR FN ++CV   G SSC   SG  S   WLS+ LD
Sbjct: 194 NAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQELD 253

Query: 248 NTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 280
           +T Q+++RWVQ N+MIYNYCTDAKRFPQG P EC
Sbjct: 254 STGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MC06g_new0096 vs. TAIR 10
Match: AT5G57550.1 (xyloglucan endotransglucosylase/hydrolase 25 )

HSP 1 Score: 416.0 bits (1068), Expect = 2.4e-116
Identity = 194/278 (69.78%), Postives = 223/278 (80.22%), Query Frame = 0

Query: 6   LSLLLFLTLSV----SSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSK 65
           LSLL  LT+S     S VFA  F+ + ++ WG+ R  +L++G LLTLSLDRASGSGFQ+K
Sbjct: 8   LSLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTK 67

Query: 66  NVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTN 125
             YLFGKI+MQLKLVPGNSAGTVT YYLKS+G TWDEIDFEFLGNLTGDPYT+HTNV+T 
Sbjct: 68  KEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQ 127

Query: 126 GVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMR 185
           G G REQQFHLWFDPT DFHTYS+LWNP  I+F VD  PVREFKN +  G+ +P  QPMR
Sbjct: 128 GKGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMR 187

Query: 186 LYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWLS 245
           LYSSLWNAD WATRGGLVKTDWSKAPFTA+YRNF AD+CV S G SSC +G+P    W S
Sbjct: 188 LYSSLWNADQWATRGGLVKTDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPR---WFS 247

Query: 246 ENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 280
           + LD T + ++R VQ+ +MIYNYCTD KRFPQGFP EC
Sbjct: 248 QRLDLTAEDKMRVVQRKYMIYNYCTDTKRFPQGFPKEC 282

BLAST of MC06g_new0096 vs. TAIR 10
Match: AT4G30270.1 (xyloglucan endotransglucosylase/hydrolase 24 )

HSP 1 Score: 406.8 bits (1044), Expect = 1.5e-113
Identity = 188/283 (66.43%), Postives = 221/283 (78.09%), Query Frame = 0

Query: 1   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 60
           M P  +     L ++  SV AA+FN DV + WGN R  IL++G LLTLSLD++SGSGFQS
Sbjct: 1   MSPFKIFFFTTLLVAAFSVSAADFNTDVNVAWGNGRGKILNNGQLLTLSLDKSSGSGFQS 60

Query: 61  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 120
           K  YLFGKI+MQ+KLVPGNSAGTVTT+YLKSEGSTWDEIDFEFLGN++GDPYTLHTNV+T
Sbjct: 61  KTEYLFGKIDMQIKLVPGNSAGTVTTFYLKSEGSTWDEIDFEFLGNMSGDPYTLHTNVYT 120

Query: 121 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 180
            G G +EQQFHLWFDPT +FHTYSILWNPQRII  VD TP+REFKN ES GV+FP N+PM
Sbjct: 121 QGKGDKEQQFHLWFDPTANFHTYSILWNPQRIILTVDDTPIREFKNYESLGVLFPKNKPM 180

Query: 181 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL 240
           R+Y+SLWNADDWATRGGLVKTDWSKAPF A+YRN   DS                   W 
Sbjct: 181 RMYASLWNADDWATRGGLVKTDWSKAPFMASYRNIKIDS--------------KPNSNWY 240

Query: 241 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNA 284
           ++ +D+T Q RL+WVQKN+MIYNYCTD +RFPQG P EC+ ++
Sbjct: 241 TQEMDSTSQARLKWVQKNYMIYNYCTDHRRFPQGAPKECTTSS 269

BLAST of MC06g_new0096 vs. TAIR 10
Match: AT5G48070.1 (xyloglucan endotransglucosylase/hydrolase 20 )

HSP 1 Score: 396.0 bits (1016), Expect = 2.6e-110
Identity = 176/277 (63.54%), Postives = 220/277 (79.42%), Query Frame = 0

Query: 5   ALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDD-GHLLTLSLDRASGSGFQSKNV 64
           A  ++         V+A +F++DV++ WG+ R  ILD+ G+LL+LSLD+ SGSGFQS   
Sbjct: 10  AFLIIFLFAAQYERVYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQE 69

Query: 65  YLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGV 124
           +L+GK+E+Q+KLVPGNSAGTVTT+YLKS G+TWDEIDFEFLGN++G PYTLHTNV+T G 
Sbjct: 70  FLYGKVEVQMKLVPGNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGT 129

Query: 125 GKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLY 184
           G +EQQFHLWFDPT DFHTY I+WNPQR+IF +DG P+REFKN E+ GV FP +QPMRLY
Sbjct: 130 GDKEQQFHLWFDPTVDFHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLY 189

Query: 185 SSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWLSEN 244
           +SLW A+ WATRGGL KTDWSKAPFTA YRN+N D+CVWS G SSCS+ +     W ++ 
Sbjct: 190 ASLWEAEHWATRGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANS----SWFTQV 249

Query: 245 LDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECS 281
           LD   + R++W Q+ +M+YNYCTD KRFPQG PPECS
Sbjct: 250 LDFKGKNRVKWAQRKYMVYNYCTDKKRFPQGAPPECS 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q388576.0e-12071.64Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=... [more]
Q389101.9e-11873.72Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis tha... [more]
Q389073.4e-11569.78Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis tha... [more]
P248062.1e-11266.43Xyloglucan endotransglucosylase/hydrolase protein 24 OS=Arabidopsis thaliana OX=... [more]
Q9FI313.7e-10963.54Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
XP_022135083.11.93e-216100.00xyloglucan endotransglucosylase/hydrolase protein 22-like [Momordica charantia][more]
CCH26406.11.21e-17482.62xyloglucan endotransglycosylase [Cucumis sativus][more]
XP_004146309.11.41e-17382.27xyloglucan endotransglucosylase/hydrolase protein 22 [Cucumis sativus] >KGN65439... [more]
XP_038880531.17.58e-17381.91xyloglucan endotransglucosylase/hydrolase protein 22-like [Benincasa hispida][more]
NP_001284442.11.64e-17281.27xyloglucan endotransglucosylase/hydrolase protein 22-like precursor [Cucumis mel... [more]
Match NameE-valueIdentityDescription
A0A6J1C3T79.33e-217100.00Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC1... [more]
N0DX335.86e-17582.62Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth6 PE=... [more]
A0A0A0LWT16.83e-17482.27Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G42... [more]
Q06BI57.96e-17381.27Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=XTH2 PE=2 S... [more]
A0A6J1KN181.47e-17181.43Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G57560.14.3e-12171.64Xyloglucan endotransglucosylase/hydrolase family protein [more]
AT4G25810.11.4e-11973.72xyloglucan endotransglycosylase 6 [more]
AT5G57550.12.4e-11669.78xyloglucan endotransglucosylase/hydrolase 25 [more]
AT4G30270.11.5e-11366.43xyloglucan endotransglucosylase/hydrolase 24 [more]
AT5G48070.12.6e-11063.54xyloglucan endotransglucosylase/hydrolase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008264Beta-glucanasePRINTSPR00737GLHYDRLASE16coord: 132..149
score: 46.76
coord: 56..74
score: 25.44
coord: 114..127
score: 46.43
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 4..284
e-value: 6.6E-117
score: 387.8
IPR016455Xyloglucan endotransglucosylase/hydrolaseCDDcd02176GH16_XETcoord: 21..279
e-value: 4.47735E-151
score: 421.223
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 237..279
e-value: 6.1E-22
score: 77.2
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 25..205
e-value: 1.6E-59
score: 200.4
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 1..212
score: 31.424942
NoneNo IPR availableGENE3D2.60.120.200coord: 12..284
e-value: 3.6E-109
score: 366.4
NoneNo IPR availablePIRSRPIRSR037299-1PIRSR037299-1coord: 41..217
e-value: 3.0E-29
score: 100.1
NoneNo IPR availablePANTHERPTHR31062:SF178XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASEcoord: 8..280
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 8..280
IPR008263Glycoside hydrolase, family 16, active sitePROSITEPS01034GH16_1coord: 98..108
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 24..282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0096.1MC06g_new0096.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0071555 cell wall organization
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity