MC06g1781 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g1781
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Aspartic proteinase nepenthesin-2-like
Location	MC06: 25090863 .. 25091328 (-)
RNA-Seq Expression	MC06g1781
Synteny	MC06g1781

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTCCTTCCCATTCCGATTCCCTTTCTATTTTCATATTCCATTGCTTCTACTTCCTCTGCTTCCTCTAACACCACCATCACACTCCCCCTCACCGCCTTCTCTTCTCTCGAGTTCCAGATCCATGGAAATGGTCCAAGAACCGAAACAAATCAAGCGCATTCGCCGAGAAATTTGCTCACGTGTAGCTATGGCGCTTACTCAGTTTTTCTCAGCTTCGGAAGTAGTCTCGTCTGGTTCCCCTGCACCGCCCATTTTCTCTGCTTCAGGTGTTCGTTTCCGAACGCGTGGCTATTGCGACGATTACGAAATTTATCCCCAAATTATCTTCTGCTGCGAAGATTATCGGTTACGGAAAGTGGAAATGTGCTTGGATTTTTGGCCCTAATGTGAAATTTAGATGTTGGAATGCCGTCTTAATTCTTGAAATTGTTCCGATACGTGTCCTGGTTATGGAATTAAG

mRNA sequence

ATGGAATTCCTTCCCATTCCGATTCCCTTTCTATTTTCATATTCCATTGCTTCTACTTCCTCTGCTTCCTCTAACACCACCATCACACTCCCCCTCACCGCCTTCTCTTCTCTCGAGTTCCAGATCCATGGAAATGGTCCAAGAACCGAAACAAATCAAGCGCATTCGCCGAGAAATTTGCTCACGTGTAGCTATGGCGCTTACTCAGTTTTTCTCAGCTTCGGAAGTAGTCTCGTCTGGTTCCCCTGCACCGCCCATTTTCTCTGCTTCAGGTGTTCGTTTCCGGTGGCTATTGCGACGATTACGAAATTTATCCCCAAATTATCTTCTGCTGCGAAGATTATCGGTTACGGAAAGTGGAAATGTGCTTGGATTTTTGGCCCTAATGTGAAATTTAGATGTTGGAATGCCGTCTTAATTCTTGAATGTTCCGATACGTGTCCTGGTTATGGAATTAAG

Coding sequence (CDS)

ATGGAATTCCTTCCCATTCCGATTCCCTTTCTATTTTCATATTCCATTGCTTCTACTTCCTCTGCTTCCTCTAACACCACCATCACACTCCCCCTCACCGCCTTCTCTTCTCTCGAGTTCCAGATCCATGGAAATGGTCCAAGAACCGAAACAAATCAAGCGCATTCGCCGAGAAATTTGCTCACGTGTAGCTATGGCGCTTACTCAGTTTTTCTCAGCTTCGGAAGTAGTCTCGTCTGGTTCCCCTGCACCGCCCATTTTCTCTGCTTCAGGTGTTCGTTTCCGGTGGCTATTGCGACGATTACGAAATTTATCCCCAAATTATCTTCTGCTGCGAAGATTATCGGTTACGGAAAGTGGAAATGTGCTTGGATTTTTGGCCCTAATGTGAAATTTAGATGTTGGAATGCCGTCTTAATTCTTGAATGTTCCGATACGTGTCCTGGTTATGGAATTAAG

Protein sequence

MEFLPIPIPFLFSYSIASTSSASSNTTITLPLTAFSSLEFQIHGNGPRTETNQAHSPRNLLTCSYGAYSVFLSFGSSLVWFPCTAHFLCFRCSFPVAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWNAVLILECSDTCPGYGIK

Homology

[Close]

BLAST of MC06g1781 vs. NCBI nr
Match: XP_011657732.1 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN48299.1 hypothetical protein Csa_004059 [Cucumis sativus])

HSP 1 Score: 139 bits (350), Expect = 7.77e-36
Identity = 87/185 (47.03%), Postives = 111/185 (60.00%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIASTSSASSNTTITLPLTAFSSLEFQI-----------------H 60
           MEFLPIP  FLFS  +   +S+SS+TT+ LPLT F S+ F                   H
Sbjct: 1   MEFLPIP--FLFSIFLLLPTSSSSSTTV-LPLTTFPSVSFTDPFKTINLLLSASLNRAQH 60

Query: 61  GNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLCF 120
              P++++N +    +L   SYGAYSV L+FG             SSLVWFPCTA + C 
Sbjct: 61  LKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCS 120

Query: 121 RCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWNA-VLILECSDTCP 153
           RCSFP V  ATI+KF+PKLSS+ K++G    KCAWIFGPN+K RC N      +CSD+CP
Sbjct: 121 RCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCP 180

[Close]

BLAST of MC06g1781 vs. NCBI nr
Match: XP_023543736.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 139 bits (349), Expect = 1.13e-35
Identity = 91/186 (48.92%), Postives = 108/186 (58.06%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIA-STSSASSNTTITLPLTAFSSLEFQI----------------- 60
           MEF PIP  FL S  +  S SS+SS+TT+TLPLT F SL F                   
Sbjct: 1   MEFFPIP--FLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFTHPWKNIKHLVSASLTRAQ 60

Query: 61  HGNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLC 120
           H   PR ++N +     L   SYGAYS+ L+FG             SSLVWFPCTA + C
Sbjct: 61  HLKTPRIKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRC 120

Query: 121 FRCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWN-AVLILECSDTC 153
             CSFP V  ATI KFIPKLSS+AKIIG    KC+WIFGPN+K  C + +    +CSDTC
Sbjct: 121 SNCSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKSLCRSCSPRSRKCSDTC 180

[Close]

BLAST of MC06g1781 vs. NCBI nr
Match: XP_022925946.1 (probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 137 bits (345), Expect = 4.29e-35
Identity = 91/186 (48.92%), Postives = 108/186 (58.06%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIA-STSSASSNTTITLPLTAFSSLEFQI----------------- 60
           MEF  IP  FL S  +  S SS+SS+TT+TLPLT F SL F                   
Sbjct: 1   MEFFLIP--FLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQ 60

Query: 61  HGNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLC 120
           H   PRT++N +     L   SYGAYS+ L+FG             SSLVWFPCTA + C
Sbjct: 61  HLKTPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRC 120

Query: 121 FRCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWN-AVLILECSDTC 153
             CSFP V  ATI KFIPKLSS+AKIIG    KC+WIFGPN+K  C + +    +CSDTC
Sbjct: 121 SNCSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTC 180

[Close]

BLAST of MC06g1781 vs. NCBI nr
Match: KAG7034471.1 (Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 136 bits (343), Expect = 8.35e-35
Identity = 91/186 (48.92%), Postives = 108/186 (58.06%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIA-STSSASSNTTITLPLTAFSSLEFQI----------------- 60
           MEF  IP  FL S  +  S SS+SS+TT+TLPLT F SL F                   
Sbjct: 1   MEFFLIP--FLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQ 60

Query: 61  HGNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLC 120
           H   PRT++N +     L   SYGAYS+ L+FG             SSLVWFPCTA + C
Sbjct: 61  HLKIPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRC 120

Query: 121 FRCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWN-AVLILECSDTC 153
             CSFP V  ATI KFIPKLSS+AKIIG    KC+WIFGPN+K  C + +    +CSDTC
Sbjct: 121 SNCSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTC 180

[Close]

BLAST of MC06g1781 vs. NCBI nr
Match: TYK07446.1 (aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 136 bits (342), Expect = 1.10e-34
Identity = 87/185 (47.03%), Postives = 109/185 (58.92%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIASTSSASSNTTITLPLTAFSSLEFQI-----------------H 60
           MEFLPIP  FLFS  +   +S+SS+  ITLPLT F S+ F                   H
Sbjct: 1   MEFLPIP--FLFSIFLLLPTSSSSS--ITLPLTTFPSIPFTDPLKTINHLLSASLSRAQH 60

Query: 61  GNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLCF 120
              P++++N +    +L   SYGAY+V L+FG             SSLVWFPCTA + C 
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 RCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWNA-VLILECSDTCP 153
            CSFP V  ATI+KF+PKLSS+ KI+G    KCAWIFGPN+K RC N      +CSD+CP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

[Close]

BLAST of MC06g1781 vs. ExPASy TrEMBL
Match: A0A0A0KHK2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454470 PE=3 SV=1)

HSP 1 Score: 139 bits (350), Expect = 3.76e-36
Identity = 87/185 (47.03%), Postives = 111/185 (60.00%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIASTSSASSNTTITLPLTAFSSLEFQI-----------------H 60
           MEFLPIP  FLFS  +   +S+SS+TT+ LPLT F S+ F                   H
Sbjct: 1   MEFLPIP--FLFSIFLLLPTSSSSSTTV-LPLTTFPSVSFTDPFKTINLLLSASLNRAQH 60

Query: 61  GNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLCF 120
              P++++N +    +L   SYGAYSV L+FG             SSLVWFPCTA + C 
Sbjct: 61  LKTPQSKSNTSIQNVSLFPRSYGAYSVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCS 120

Query: 121 RCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWNA-VLILECSDTCP 153
           RCSFP V  ATI+KF+PKLSS+ K++G    KCAWIFGPN+K RC N      +CSD+CP
Sbjct: 121 RCSFPYVDPATISKFVPKLSSSVKVVGCRNPKCAWIFGPNLKSRCRNCNSKSRKCSDSCP 180

[Close]

BLAST of MC06g1781 vs. ExPASy TrEMBL
Match: A0A6J1EDJ0 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111433208 PE=3 SV=1)

HSP 1 Score: 137 bits (345), Expect = 2.08e-35
Identity = 91/186 (48.92%), Postives = 108/186 (58.06%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIA-STSSASSNTTITLPLTAFSSLEFQI----------------- 60
           MEF  IP  FL S  +  S SS+SS+TT+TLPLT F SL F                   
Sbjct: 1   MEFFLIP--FLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQ 60

Query: 61  HGNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLC 120
           H   PRT++N +     L   SYGAYS+ L+FG             SSLVWFPCTA + C
Sbjct: 61  HLKTPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRC 120

Query: 121 FRCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWN-AVLILECSDTC 153
             CSFP V  ATI KFIPKLSS+AKIIG    KC+WIFGPN+K  C + +    +CSDTC
Sbjct: 121 SNCSFPNVDAATIPKFIPKLSSSAKIIGCRNRKCSWIFGPNLKTLCRSCSPRSRKCSDTC 180

[Close]

BLAST of MC06g1781 vs. ExPASy TrEMBL
Match: A0A5D3CAS4 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001760 PE=3 SV=1)

HSP 1 Score: 136 bits (342), Expect = 5.34e-35
Identity = 87/185 (47.03%), Postives = 109/185 (58.92%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIASTSSASSNTTITLPLTAFSSLEFQI-----------------H 60
           MEFLPIP  FLFS  +   +S+SS+  ITLPLT F S+ F                   H
Sbjct: 1   MEFLPIP--FLFSIFLLLPTSSSSS--ITLPLTTFPSIPFTDPLKTINHLLSASLSRAQH 60

Query: 61  GNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLCF 120
              P++++N +    +L   SYGAY+V L+FG             SSLVWFPCTA + C 
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 RCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWNA-VLILECSDTCP 153
            CSFP V  ATI+KF+PKLSS+ KI+G    KCAWIFGPN+K RC N      +CSD+CP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

[Close]

BLAST of MC06g1781 vs. ExPASy TrEMBL
Match: A0A5A7SGF9 (Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00670 PE=3 SV=1)

HSP 1 Score: 134 bits (337), Expect = 2.82e-34
Identity = 86/185 (46.49%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIASTSSASSNTTITLPLTAFSSLEFQI-----------------H 60
           MEFLPIP  FLFS  +   +S+SS+  ITLPL  F S+ F                   H
Sbjct: 1   MEFLPIP--FLFSIFLLLPTSSSSS--ITLPLATFPSIPFTDPLKTINHLLSASLSRAQH 60

Query: 61  GNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLCF 120
              P++++N +    +L   SYGAY+V L+FG             SSLVWFPCTA + C 
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 RCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWNA-VLILECSDTCP 153
            CSFP V  ATI+KF+PKLSS+ KI+G    KCAWIFGPN+K RC N      +CSD+CP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

[Close]

BLAST of MC06g1781 vs. ExPASy TrEMBL
Match: A0A1S3CHV2 (aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 PE=3 SV=1)

HSP 1 Score: 134 bits (337), Expect = 2.82e-34
Identity = 86/185 (46.49%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 1   MEFLPIPIPFLFSYSIASTSSASSNTTITLPLTAFSSLEFQI-----------------H 60
           MEFLPIP  FLFS  +   +S+SS+  ITLPL  F S+ F                   H
Sbjct: 1   MEFLPIP--FLFSIFLLLPTSSSSS--ITLPLATFPSIPFTDPLKTINHLLSASLSRAQH 60

Query: 61  GNGPRTETNQAHSPRNLLTCSYGAYSVFLSFG-------------SSLVWFPCTAHFLCF 120
              P++++N +    +L   SYGAY+V L+FG             SSLVWFPCTA + C 
Sbjct: 61  LKSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCA 120

Query: 121 RCSFP-VAIATITKFIPKLSSAAKIIGYGKWKCAWIFGPNVKFRCWNA-VLILECSDTCP 153
            CSFP V  ATI+KF+PKLSS+ KI+G    KCAWIFGPN+K RC N      +CSD+CP
Sbjct: 121 HCSFPHVDPATISKFVPKLSSSVKIVGCRNPKCAWIFGPNLKSRCRNCNPKSRKCSDSCP 180

[Close]

BLAST of MC06g1781 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.1e-13
Identity = 45/101 (44.55%), Postives = 56/101 (55.45%), Query Frame = 0


Query: 64  SYGAYSVFLSF-------------GSSLVWFPCTAHFLCFRCSFPVAIAT-ITKFIPKLS 123
           SYG YSV LSF             GSSLVW PCT+ +LC  C F     T I +FIPK S
Sbjct: 86  SYGGYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTSRYLCSGCDFSGLDPTLIPRFIPKNS 145

Query: 124 SAAKIIGYGKWKCAWIFGPNVKFRCWNAVLILECSDTCPGY 151
           S++KIIG    KC +++GPNV+ R  +      C+  CP Y
Sbjct: 146 SSSKIIGCQSPKCQFLYGPNVQCRGCDP-NTRNCTVGCPPY 185

The following BLAST results are available for this feature:

BLAST of MC06g1781 vs.


Match Name	E-value	Identity	Description

BLAST of MC06g1781 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011657732.1	7.77e-36	47.03	probable aspartyl protease At4g16563 [Cucumis sativus] >KGN48299.1 hypothetical ...	[more]
XP_023543736.1	1.13e-35	48.92	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_022925946.1	4.29e-35	48.92	probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic...	[more]
KAG7034471.1	8.35e-35	48.92	Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
TYK07446.1	1.10e-34	47.03	aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa]	[more]

BLAST of MC06g1781 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHK2	3.76e-36	47.03	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G45447...	[more]
A0A6J1EDJ0	2.08e-35	48.92	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114332...	[more]
A0A5D3CAS4	5.34e-35	47.03	Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A5A7SGF9	2.82e-34	46.49	Aspartic proteinase nepenthesin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CHV2	2.82e-34	46.49	aspartic proteinase nepenthesin-2-like OS=Cucumis melo OX=3656 GN=LOC103500932 P...	[more]

BLAST of MC06g1781 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52500.1	2.1e-13	44.55	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 67..152
None	No IPR available	PANTHER	PTHR13683:SF710	OS03G0281400 PROTEIN	coord: 67..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g1781.1	MC06g1781.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity