MC06g1710 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1710
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionbifunctional pinoresinol-lariciresinol reductase-like
LocationMC06: 24506162 .. 24509803 (+)
RNA-Seq ExpressionMC06g1710
SyntenyMC06g1710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGGGGAAGAGCAAAGTTTTAATAGTGGGAGGAACAGGGTATTTGGGGAAGAGATTGGTGAGGGCAAGTTTGGGATTAGGGCATGAAACATATGTTTTACAGAGGGCAGAAATGGGAGTTGAGATTGAAAAAGTGGAGCTTCTTCTTTCATTCAAGAAACAAGGAGCCCATTTGGTTTGTGCTTCTTTTAATGACCATAATACCCTTGTGGAGGCTGTCAAGCTTGTGGATGTTGTCATCTCTGCAATTTCTGGTGTTCATATCAGATCCCATCAAATCCTTCTTCAACTCAAACTTGTTGATGCCATTAAACAGGCTGGAAATATCAAGGTTCCTTCTCTCTCTCCCCCTTCTGTATGTAATGTATATATATATTAATACTCATATGGTGTCAGAACTGCAAACGTTCAAACGAGTATGGAATCTGAACAATATCGATGAGTCAAGTGAATCATGATCAGAGCAGGCAAAGAGTAGGGGTGAGCATCGATCGGTTGGAGTCGGTTTTTTGCTGACCCCAACGCTACTTTTCTATTACGACGGCTTCCACCGACCGACCGTCGTTTCGGTCGATCGATTTCCTCGGTTTCTGTGAACATTTTTTTTGTCGTGGCGCCGTTGGTGACAATATTGGAGCCATGACTGATTTCTGCCCAAAACAAAAAAGAAAAAGAAGTAGAGAAAGATCTAGACGGTAGGCGAGCTTCAGTGCGATTCAAGATACTGGAAGAGGAAAAATGAAGGAAAAATGAAGAAGAATATTAAGAAGAAAAATGGAGAGCCTGGGAAGAAGAAGAATGAGGAAGATGAAGTGTGGCGAGGCGAGGGAGGAAAGGAGAAGGAAAAAAGACAAAAGTGAAAGAGGAGAAAGAGGGGAAAGGAAACAAATTTATATAAAGTTAAAAAATTAAAAAATTAAATTTTTTTTTTCTAAAACGTACCTTGAAAACTATTCGTGTGGTTGGTTTTCAATGCGCTGCTGCAGTGCAGTTTCGCTGCAGCATGCATGACGCAGTCTGTTTTTCTTTTAAATCAACACATGTCGGTCGGTTCAGTTGAAAATCATTTTTCTGACCGACTAATAGATAGGTTTTATAATTAAGAAATCGATTCCTACCAGATTTTTCATCAAATTGATCGATCGACGTTGATTCGGTCAGTTTTGATCGGTCGGATAGGTTTTTTAGAGCTTTTTGCTCACTCTTAACAAAGAAAATAGACCTAACAAAAGTTTATAATAGACATGAGTTACTACCTCATCTTATTGTCAATTAGTTTTAAGATGAAACTCTTGAATATTAATAACCTAGGTCCCCCAACCAAATTCTTACCAAAACTTCGTGTGCATTATTGTACACCCAGCTTTCTGAAATAGGCTATTACGATAACCATAGATATTGTTCTCTTGACCGTATCAACATCATTTAAAAATAACCTTGAATTCAAATCCGCATGGAATAATTATTTATAGAGAAAAAGGTATAGTGTAAACAAAGGAGTGCCACTCTCTTCCATTTTTCTCATTGTCTCTCCAAATCCCTATTATCACTCCCCTTGATCTTCTCTCTCCCCTTCCCTATTTCTAATTTAATTTTTTTTTCCTTCTCTCACATGCAAAATTACACAATACCATCTCTCACATGTAAGATCTCTCCCCCTTTCAATGCACAACCACACTCTCATGAGTAAATTTAGGAAACTTTCTAGCTAGCTAGTGGTATGAAAACTTATTTTCACCGACGGTATTGTTTGCCGCATCAACAACATTTAAGAATGATGTATAGAATCCATGAAATAAAATAATAAATTACTTAAAGATACGTCAAATTGTAGGTAGAAGTGGTAGATACACGATAGTACGTACTAGAATTTTTTTCAAAAATTTTCTACTCGGATTAAAATACCAAAAGGAAACACAAGATCATAATGCCTAATACGATTATAAAGTAGTTATTGTTGTATTTTGTACAGAGATTTTTGCCATCCGAGTTCGGGACGGATCCAGCAAGAATGGAGAATGCAACGGAGCCGGGAAGAGCAACATTTGATGATAAAATGGTGGTGAGGAAAGCCATAGAAGAGGCTAAAATTCCCTTCACTTACATCTCTGCAAATTGCTTTGCTGGTTACTTTCTTGGTGGTTTGTGTCAGCCTGGTTCCATCCTTCCCTCCAAAGATCATGTTCTCCTCCTTGGAGATGCCAACCAAAAAGGTAACTAAACCTATACATATACACAAATACATACATATATACACATGTACACATCATATGTTTCTTTTACTAGCTACTAAATGTGTGGAGTGAGGATTCAAATCCAGCCTAAGAACAAATCTAGGGCGGAGCTAGAAATTTTTTGTACCTTCTAACTTTTCATATATATAAATTTTCAATTTCGAAAGTAATTTAGAGAGGTCAAATTAAAGAGAGCAACTTTAAAATCTTAATTTCTTTTTAATTTGAGACAGACTTTTAGAGAGATTATTAAAGCTTTATTCAATTTAATTATGCTTAGGTTGGTTTTGCATATCATATTTATCTGACTTTCACTGTGAAAGTCTCTCTTCATAGAAACTGTCATTTCACACTTAGGATGAGTTTGGTATATCTTTAATTTAAACATTAGAATATCAATCACTTGAAATAACTTTTTAGATAGTCATCCCAACTGTCTCTTAAAAAATAGATCTTAAATTAATTTAATTTTTATTTATTTATTATCGAATGTTTTGTTGTGGTAGAGAAAATATATATTTATTTAAAAAGCACTTTTTTTAAGTCGTTGGGTAAACACATTGTACTAATATAATTGTGATAAATTGTGACAGCAATTTATGTGGATGAAGATGACATTGCAATGTATACCATGAAAACCATTGATGACCCTCGAACTCTGAACAAAACAGTGTACATTAGGCCACCAAAGAACATATTATCACAAAGAGAAGTTGTTGAGATTTGGGAGAAATTAATTGGGAAGCAGCTCACAAAGACCTCCATTTCTGCTCACCAATTTCTTGCAAAAATCAAAGGTCAGCTCTATCATCATTCATTTTCCCTCTCTTTCTATCATATCAATTAGTTTTCTGTTATTATTTAGCATCACTCATTAATGATAAAGATTGATAAATTAAAGTGGTAAGAATTAAATCAACACCACCGTAAAGTGAAAATTTTCTTTTTTCTTTTATTTTTTTGGGAAATAAAAAATAGGTGAAATTAGCTACGCAGAGCAAGTTGGGCTAAGTCACTATTATGATGTATGCTATGAGGGTTGTCTGGCAAACTTTGAAATAGGGAAGGATGGTGAAGAAGCCTGCAATCTGTATCCAGAAATCAACTACACAACTGCTGAGGATTACATGAAACGCTACCTTTAGGCTTTTAACACCTGCCAAATTATAAACCCTCAAAATAAAACCACATTCATTTTATTTCAGTTATGTTTCCTTCCCCGACTGCCCTACCCAATTATGATATCGATGTACCTATCCAATGTCGGATTTGAATAAAGATGTAAGATTGTGTTTCATGAGCGTTTTATAACCATTCTTGTTTCTTGTTTCTCGTCTAAAAGAAATGTTTAATAACTATTTTTGTTTC

mRNA sequence

CAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGGGGAAGAGCAAAGTTTTAATAGTGGGAGGAACAGGGTATTTGGGGAAGAGATTGGTGAGGGCAAGTTTGGGATTAGGGCATGAAACATATGTTTTACAGAGGGCAGAAATGGGAGTTGAGATTGAAAAAGTGGAGCTTCTTCTTTCATTCAAGAAACAAGGAGCCCATTTGGTTTGTGCTTCTTTTAATGACCATAATACCCTTGTGGAGGCTGTCAAGCTTGTGGATGTTGTCATCTCTGCAATTTCTGGTGTTCATATCAGATCCCATCAAATCCTTCTTCAACTCAAACTTGTTGATGCCATTAAACAGGCTGGAAATATCAAGAGATTTTTGCCATCCGAGTTCGGGACGGATCCAGCAAGAATGGAGAATGCAACGGAGCCGGGAAGAGCAACATTTGATGATAAAATGGTGGTGAGGAAAGCCATAGAAGAGGCTAAAATTCCCTTCACTTACATCTCTGCAAATTGCTTTGCTGGTTACTTTCTTGGTGGTTTGTGTCAGCCTGGTTCCATCCTTCCCTCCAAAGATCATGTTCTCCTCCTTGGAGATGCCAACCAAAAAGCAATTTATGTGGATGAAGATGACATTGCAATGTATACCATGAAAACCATTGATGACCCTCGAACTCTGAACAAAACAGTGTACATTAGGCCACCAAAGAACATATTATCACAAAGAGAAGTTGTTGAGATTTGGGAGAAATTAATTGGGAAGCAGCTCACAAAGACCTCCATTTCTGCTCACCAATTTCTTGCAAAAATCAAAGGTGAAATTAGCTACGCAGAGCAAGTTGGGCTAAGTCACTATTATGATGTATGCTATGAGGGTTGTCTGGCAAACTTTGAAATAGGGAAGGATGGTGAAGAAGCCTGCAATCTGTATCCAGAAATCAACTACACAACTGCTGAGGATTACATGAAACGCTACCTTTAGGCTTTTAACACCTGCCAAATTATAAACCCTCAAAATAAAACCACATTCATTTTATTTCAGTTATGTTTCCTTCCCCGACTGCCCTACCCAATTATGATATCGATGTACCTATCCAATGTCGGATTTGAATAAAGATGTAAGATTGTGTTTCATGAGCGTTTTATAACCATTCTTGTTTCTTGTTTCTCGTCTAAAAGAAATGTTTAATAACTATTTTTGTTTC

Coding sequence (CDS)

ATGGGGAAGAGCAAAGTTTTAATAGTGGGAGGAACAGGGTATTTGGGGAAGAGATTGGTGAGGGCAAGTTTGGGATTAGGGCATGAAACATATGTTTTACAGAGGGCAGAAATGGGAGTTGAGATTGAAAAAGTGGAGCTTCTTCTTTCATTCAAGAAACAAGGAGCCCATTTGGTTTGTGCTTCTTTTAATGACCATAATACCCTTGTGGAGGCTGTCAAGCTTGTGGATGTTGTCATCTCTGCAATTTCTGGTGTTCATATCAGATCCCATCAAATCCTTCTTCAACTCAAACTTGTTGATGCCATTAAACAGGCTGGAAATATCAAGAGATTTTTGCCATCCGAGTTCGGGACGGATCCAGCAAGAATGGAGAATGCAACGGAGCCGGGAAGAGCAACATTTGATGATAAAATGGTGGTGAGGAAAGCCATAGAAGAGGCTAAAATTCCCTTCACTTACATCTCTGCAAATTGCTTTGCTGGTTACTTTCTTGGTGGTTTGTGTCAGCCTGGTTCCATCCTTCCCTCCAAAGATCATGTTCTCCTCCTTGGAGATGCCAACCAAAAAGCAATTTATGTGGATGAAGATGACATTGCAATGTATACCATGAAAACCATTGATGACCCTCGAACTCTGAACAAAACAGTGTACATTAGGCCACCAAAGAACATATTATCACAAAGAGAAGTTGTTGAGATTTGGGAGAAATTAATTGGGAAGCAGCTCACAAAGACCTCCATTTCTGCTCACCAATTTCTTGCAAAAATCAAAGGTGAAATTAGCTACGCAGAGCAAGTTGGGCTAAGTCACTATTATGATGTATGCTATGAGGGTTGTCTGGCAAACTTTGAAATAGGGAAGGATGGTGAAGAAGCCTGCAATCTGTATCCAGAAATCAACTACACAACTGCTGAGGATTACATGAAACGCTACCTTTAG

Protein sequence

MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEINYTTAEDYMKRYL
Homology
BLAST of MC06g1710 vs. ExPASy Swiss-Prot
Match: E6Y2X0 (Bifunctional pinoresinol-lariciresinol reductase 2 OS=Linum usitatissimum OX=4006 GN=PLR_Lu2 PE=1 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 2.9e-139
Identity = 244/311 (78.46%), Postives = 272/311 (87.46%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           KSKVL++GGTGYLGKRLV ASL  GHETYVLQR E+GV+IEK++LLLSFKK GA LV  S
Sbjct: 21  KSKVLVIGGTGYLGKRLVTASLAAGHETYVLQRPEIGVDIEKIQLLLSFKKAGASLVSGS 80

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
           FND+ +LV+AVKLVDVVI A+SGVHIRSHQILLQLKLVDAIK+AGN+KRFLPSEFGTDPA
Sbjct: 81  FNDYRSLVDAVKLVDVVICAVSGVHIRSHQILLQLKLVDAIKEAGNVKRFLPSEFGTDPA 140

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVL 182
            MENA EPGR TFDDKMVVRKAIEEA IPFTYISANCFAGYFLGGLCQPG ILPS++ V 
Sbjct: 141 TMENAMEPGRVTFDDKMVVRKAIEEAGIPFTYISANCFAGYFLGGLCQPGFILPSREQVT 200

Query: 183 LLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 242
           LLGD NQKA+YVDEDDIA YT+K IDDPRTLNKTVYI+PPKN+LSQREVV IWEK IGK+
Sbjct: 201 LLGDGNQKAVYVDEDDIARYTIKMIDDPRTLNKTVYIKPPKNVLSQREVVGIWEKYIGKE 260

Query: 243 LTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEINY 302
           L KT++S  +FLA +K E  YAEQVGL+HYY VCYEGCL NFEIG +  EA  LYPE+ Y
Sbjct: 261 LKKTTLSVEEFLAMMK-EQDYAEQVGLTHYYHVCYEGCLTNFEIGDEAGEATKLYPEVGY 320

Query: 303 TTAEDYMKRYL 314
           TT  +YMKRY+
Sbjct: 321 TTVVEYMKRYV 330

BLAST of MC06g1710 vs. ExPASy Swiss-Prot
Match: B5KRH5 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum corymbulosum OX=480358 GN=PLR_Lc1 PE=1 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 3.9e-136
Identity = 238/310 (76.77%), Postives = 272/310 (87.74%), Query Frame = 0

Query: 4   SKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCASF 63
           SKVL++GGTGYLGKRLV+ASL  GH+TYV+ R E+GV+IEKV+LLLSFK QGAHLV ASF
Sbjct: 8   SKVLVIGGTGYLGKRLVKASLDAGHDTYVMHRPEIGVDIEKVQLLLSFKMQGAHLVSASF 67

Query: 64  NDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPAR 123
           +DH +LV+AV LVDVVI AISGVHIRSHQILLQLKLV AIK+AGN+KRFLPSEFGTDPAR
Sbjct: 68  DDHRSLVDAVSLVDVVICAISGVHIRSHQILLQLKLVQAIKEAGNVKRFLPSEFGTDPAR 127

Query: 124 MENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVLL 183
           M +A EPGR TFDDKMVVR+AIEEA IPFTY+SANCFAGYFLGGLCQPGSILPS+DHV L
Sbjct: 128 MGDAMEPGRVTFDDKMVVRRAIEEAAIPFTYVSANCFAGYFLGGLCQPGSILPSRDHVTL 187

Query: 184 LGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQL 243
           LGD NQK +YVDE+DIA YT+K IDDPRTLNKT+YI+PPKNILSQR+VV IWEK IGKQL
Sbjct: 188 LGDGNQKGVYVDENDIAAYTLKAIDDPRTLNKTLYIKPPKNILSQRQVVGIWEKHIGKQL 247

Query: 244 TKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEINYT 303
            KT +S   FLA +K E  YAEQVGL+HYY VCYEGCL NFE+ +D +EA  LYP++ YT
Sbjct: 248 HKTLLSEQDFLAAMK-EQDYAEQVGLTHYYHVCYEGCLTNFEVEQD-QEASKLYPDVRYT 307

Query: 304 TAEDYMKRYL 314
           T E+Y+KRY+
Sbjct: 308 TVEEYLKRYV 315

BLAST of MC06g1710 vs. ExPASy Swiss-Prot
Match: Q4R0I0 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum album OX=191219 GN=PLR1 PE=1 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 3.3e-135
Identity = 233/312 (74.68%), Postives = 275/312 (88.14%), Query Frame = 0

Query: 2   GKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCA 61
           G SKVL++GGTGYLGKRLV+ASL  GH+TYV+ R E+GV+IEKV+LLLSFK QGAHLV A
Sbjct: 17  GGSKVLVIGGTGYLGKRLVKASLDSGHDTYVMHRPEIGVDIEKVQLLLSFKMQGAHLVSA 76

Query: 62  SFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDP 121
           SF+D  +LV+AVKLVDVVI AISGVHIRSHQILLQLKLV+AIK+AGN+KRF+PSEFGTDP
Sbjct: 77  SFDDQRSLVDAVKLVDVVICAISGVHIRSHQILLQLKLVEAIKEAGNVKRFVPSEFGTDP 136

Query: 122 ARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHV 181
           ARMENA EPGR TFDDKMVVR+AIEEA IPFTY+SANCFAGYFLGGLCQPG ILPS+DHV
Sbjct: 137 ARMENAMEPGRITFDDKMVVRRAIEEAGIPFTYVSANCFAGYFLGGLCQPGYILPSRDHV 196

Query: 182 LLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGK 241
            LLGD ++K +YVDEDD A YT++ IDDPRTLNKT+Y++PPKN+LSQREVV IWEK IGK
Sbjct: 197 TLLGDGDKKGVYVDEDDTAAYTLRAIDDPRTLNKTIYVKPPKNVLSQREVVGIWEKYIGK 256

Query: 242 QLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEIN 301
           +L KT +S   FLA ++ E +YAEQVGL+HYY VCYEGCL+NFE+  D +EA  LYP+++
Sbjct: 257 ELQKTILSEQDFLATMR-EQNYAEQVGLTHYYHVCYEGCLSNFEV-DDEQEASKLYPDVH 316

Query: 302 YTTAEDYMKRYL 314
           YTT E+Y+KRY+
Sbjct: 317 YTTVEEYLKRYV 326

BLAST of MC06g1710 vs. ExPASy Swiss-Prot
Match: P93143 (Bifunctional pinoresinol-lariciresinol reductase OS=Forsythia intermedia OX=55183 GN=PLR_Fi1 PE=1 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 5.4e-130
Identity = 230/314 (73.25%), Postives = 272/314 (86.62%), Query Frame = 0

Query: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60
           MGKSKVLI+GGTGYLG+RLV+ASL  GHETY+L R E+GV+I+KVE+L+SFK QGAHLV 
Sbjct: 1   MGKSKVLIIGGTGYLGRRLVKASLAQGHETYILHRPEIGVDIDKVEMLISFKMQGAHLVS 60

Query: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120
            SF D N+LVEAVKLVDVVISAISGVHIRSHQILLQLKLV+AIK+AGN+KRFLPSEFG D
Sbjct: 61  GSFKDFNSLVEAVKLVDVVISAISGVHIRSHQILLQLKLVEAIKEAGNVKRFLPSEFGMD 120

Query: 121 PAR-MENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKD 180
           PA+ M+ A EPG+ T D+KMVVRKAIE+A IPFTY+SANCFAGYFLGGLCQ G ILPS+D
Sbjct: 121 PAKFMDTAMEPGKVTLDEKMVVRKAIEKAGIPFTYVSANCFAGYFLGGLCQFGKILPSRD 180

Query: 181 HVLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLI 240
            V++ GD N+KAIY +EDDIA Y +KTI+DPRTLNKT+YI PPKNILSQREVV+ WEKLI
Sbjct: 181 FVIIHGDGNKKAIYNNEDDIATYAIKTINDPRTLNKTIYISPPKNILSQREVVQTWEKLI 240

Query: 241 GKQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPE 300
           GK+L K ++S   FLA +K E+ YA+QVGLSHY+DV Y+GCL +FEIG D EEA  LYPE
Sbjct: 241 GKELQKITLSKEDFLASVK-ELEYAQQVGLSHYHDVNYQGCLTSFEIG-DEEEASKLYPE 300

Query: 301 INYTTAEDYMKRYL 314
           + YT+ E+Y+KRY+
Sbjct: 301 VKYTSVEEYLKRYV 312

BLAST of MC06g1710 vs. ExPASy Swiss-Prot
Match: B0LL23 (Bifunctional pinoresinol-lariciresinol reductase OS=Sinopodophyllum hexandrum OX=93608 GN=PLR PE=1 SV=2)

HSP 1 Score: 463.4 bits (1191), Expect = 2.1e-129
Identity = 229/313 (73.16%), Postives = 267/313 (85.30%), Query Frame = 0

Query: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60
           M KS+VLIVGGTGYLG+R+V+A L  GH TYVL R E+GV+I+K+++LLSFK+QGAHLV 
Sbjct: 1   MAKSRVLIVGGTGYLGRRMVKACLDQGHTTYVLHRQEVGVDIDKIQMLLSFKEQGAHLVE 60

Query: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120
            SFNDH +LVEAVKLVDVVI  ISGVHIRSHQILLQLKLV+AI++AGN+KRFLPSEFG D
Sbjct: 61  GSFNDHRSLVEAVKLVDVVICTISGVHIRSHQILLQLKLVEAIEEAGNVKRFLPSEFGMD 120

Query: 121 PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180
           PARM +A EPGRATFD+KMVVRKAIE+AKIP TY SANCFAGYFLGGLCQ G I+PSK+ 
Sbjct: 121 PARMAHAMEPGRATFDEKMVVRKAIEDAKIPHTYASANCFAGYFLGGLCQFGKIIPSKES 180

Query: 181 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240
           V+L GD N K IYVDE DIA YT+KT+DDPRTLNKT+YIRPP NILSQREVVEIWEKLIG
Sbjct: 181 VILSGDGNVKGIYVDEYDIATYTIKTMDDPRTLNKTIYIRPPANILSQREVVEIWEKLIG 240

Query: 241 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI 300
           K L K+S+S   FLA +KG +S+  Q GL+HYY V YEGCL NFE+ +DG +A  LYP++
Sbjct: 241 KVLDKSSLSEEDFLALMKG-LSHGHQAGLTHYYHVSYEGCLTNFEV-EDGVDASKLYPQV 300

Query: 301 NYTTAEDYMKRYL 314
           NYTT  +Y+KRYL
Sbjct: 301 NYTTVSEYLKRYL 311

BLAST of MC06g1710 vs. NCBI nr
Match: XP_022156026.1 (bifunctional pinoresinol-lariciresinol reductase 2-like [Momordica charantia])

HSP 1 Score: 622 bits (1603), Expect = 1.73e-224
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60
           MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC
Sbjct: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60

Query: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120
           ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD
Sbjct: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120

Query: 121 PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180
           PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH
Sbjct: 121 PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180

Query: 181 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240
           VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG
Sbjct: 181 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240

Query: 241 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI 300
           KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI
Sbjct: 241 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI 300

Query: 301 NYTTAEDYMKRYL 313
           NYTTAEDYMKRYL
Sbjct: 301 NYTTAEDYMKRYL 313

BLAST of MC06g1710 vs. NCBI nr
Match: XP_038888404.1 (bifunctional pinoresinol-lariciresinol reductase 2-like [Benincasa hispida])

HSP 1 Score: 572 bits (1473), Expect = 1.09e-204
Identity = 281/313 (89.78%), Postives = 301/313 (96.17%), Query Frame = 0

Query: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60
           MGKSKVLI+GGTGYLGKRLV+ASL LGHETYVLQR E+GV+IEK+ELLLSFKK+GAHLVC
Sbjct: 1   MGKSKVLIIGGTGYLGKRLVKASLRLGHETYVLQRQEIGVDIEKIELLLSFKKEGAHLVC 60

Query: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120
           ASFNDHNTLVEA+KLVDVVISA+SGVHIRSH ILLQLKLVDAIK+AGNIKRFLPSEFGTD
Sbjct: 61  ASFNDHNTLVEAIKLVDVVISAVSGVHIRSHHILLQLKLVDAIKEAGNIKRFLPSEFGTD 120

Query: 121 PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180
           PARMENA EPGR TFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH
Sbjct: 121 PARMENAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180

Query: 181 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240
           V+LLGD NQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKN LSQREVV+IWEKLIG
Sbjct: 181 VVLLGDGNQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNSLSQREVVDIWEKLIG 240

Query: 241 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI 300
           KQLTKTSISA +FLA +KG+ +YAEQVGL+HYY VCYEGCLANFEIGKDGEEACNLYPE+
Sbjct: 241 KQLTKTSISAQEFLANMKGQ-NYAEQVGLTHYYHVCYEGCLANFEIGKDGEEACNLYPEV 300

Query: 301 NYTTAEDYMKRYL 313
           NYT+ E+YMKRY+
Sbjct: 301 NYTSVEEYMKRYI 312

BLAST of MC06g1710 vs. NCBI nr
Match: XP_008460436.1 (PREDICTED: bifunctional pinoresinol-lariciresinol reductase 2-like [Cucumis melo] >KAA0068133.1 bifunctional pinoresinol-lariciresinol reductase 2-like [Cucumis melo var. makuwa] >TYK21053.1 bifunctional pinoresinol-lariciresinol reductase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 553 bits (1425), Expect = 2.33e-197
Identity = 273/311 (87.78%), Postives = 292/311 (93.89%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           KSKVLI+GGTGYLGKRLV+ASL LGHETYVLQR E+GV+IEK+ELLLSFKK+GAHL+C S
Sbjct: 4   KSKVLIIGGTGYLGKRLVKASLKLGHETYVLQRQEIGVDIEKIELLLSFKKKGAHLICGS 63

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
            NDHN+LVEA+KLVDVVISAISGVHIRSH ILLQL LV AIK+AGN+KRF+PSEFGTDPA
Sbjct: 64  LNDHNSLVEAIKLVDVVISAISGVHIRSHHILLQLNLVHAIKEAGNVKRFVPSEFGTDPA 123

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVL 182
           RME A EPGR TFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPG ILPSKDHVL
Sbjct: 124 RMEKAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGLILPSKDHVL 183

Query: 183 LLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 242
           LLGD NQKAIYVDEDDIAMYTMK+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ
Sbjct: 184 LLGDGNQKAIYVDEDDIAMYTMKSIDDSRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 243

Query: 243 LTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEINY 302
           LTKTSIS+ +FLA +KG+  YAEQVGLSHYY VCYEGCLANFEIGKDGEEACNLYPE+NY
Sbjct: 244 LTKTSISSQEFLANMKGQ-DYAEQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVNY 303

Query: 303 TTAEDYMKRYL 313
           TT E+YMKRYL
Sbjct: 304 TTVEEYMKRYL 313

BLAST of MC06g1710 vs. NCBI nr
Match: XP_011651690.1 (bifunctional pinoresinol-lariciresinol reductase 2 [Cucumis sativus] >KGN58400.1 hypothetical protein Csa_000743 [Cucumis sativus])

HSP 1 Score: 543 bits (1400), Expect = 1.44e-193
Identity = 272/312 (87.18%), Postives = 292/312 (93.59%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVE-IEKVELLLSFKKQGAHLVCA 62
           KSKVLI+GGTGYLGKRLV+ASL LGHETYVLQR E+GV+ IEKVELLLSFKK+GAHL+C 
Sbjct: 2   KSKVLIIGGTGYLGKRLVKASLKLGHETYVLQRQEIGVDHIEKVELLLSFKKKGAHLICG 61

Query: 63  SFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDP 122
           SFNDHNTLV+A+KLVDVVIS+ISGVHIRSH ILLQL LV AIK+AGN+KRFLPSEFGTDP
Sbjct: 62  SFNDHNTLVKAIKLVDVVISSISGVHIRSHHILLQLNLVRAIKEAGNVKRFLPSEFGTDP 121

Query: 123 ARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHV 182
           ARME+A EPGR TFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHV
Sbjct: 122 ARMEDAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHV 181

Query: 183 LLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGK 242
           LLLGD NQKAIYVDEDDIAMYTMK+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGK
Sbjct: 182 LLLGDGNQKAIYVDEDDIAMYTMKSIDDCRTLNKTVYIRPPKNILSQREVVEIWEKLIGK 241

Query: 243 QLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEIN 302
           QL KTSIS+ +FLA +K +  YA QVGLSHYY VCYEGCLANFEIGKDGEEACNLYPE++
Sbjct: 242 QLIKTSISSQEFLANMKDQ-DYAAQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVD 301

Query: 303 YTTAEDYMKRYL 313
           YTT E+YMKRYL
Sbjct: 302 YTTVEEYMKRYL 312

BLAST of MC06g1710 vs. NCBI nr
Match: XP_022963982.1 (bifunctional pinoresinol-lariciresinol reductase 2-like [Cucurbita moschata])

HSP 1 Score: 539 bits (1389), Expect = 6.85e-192
Identity = 268/313 (85.62%), Postives = 292/313 (93.29%), Query Frame = 0

Query: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60
           MGK+KVLI+GGTGYLGKRLVRASL LG++TYVLQR EMGV+IEKVELLLSFK++GAHLVC
Sbjct: 1   MGKNKVLIIGGTGYLGKRLVRASLRLGYQTYVLQRQEMGVDIEKVELLLSFKREGAHLVC 60

Query: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120
           ASFNDHN+LVEAVKLVDVVISAISGVHIRSH ILLQLKLV AIK+A N+KRFLPSEFGTD
Sbjct: 61  ASFNDHNSLVEAVKLVDVVISAISGVHIRSHHILLQLKLVHAIKEAPNVKRFLPSEFGTD 120

Query: 121 PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180
           PARM+NA EPGR TFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPG ILPSK H
Sbjct: 121 PARMKNAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGFILPSKHH 180

Query: 181 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240
           V LLGD NQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG
Sbjct: 181 VSLLGDGNQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240

Query: 241 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI 300
           K+LTK+SIS  +FLA +KG+  YAEQVGL+HYY VC+EGCLANFEIG   EEAC+LYPE+
Sbjct: 241 KELTKSSISDQEFLANMKGQ-GYAEQVGLTHYYHVCFEGCLANFEIGDGAEEACSLYPEV 300

Query: 301 NYTTAEDYMKRYL 313
           +YT+ E+YMKRY+
Sbjct: 301 SYTSVEEYMKRYV 312

BLAST of MC06g1710 vs. ExPASy TrEMBL
Match: A0A6J1DTL0 (bifunctional pinoresinol-lariciresinol reductase 2-like OS=Momordica charantia OX=3673 GN=LOC111022994 PE=4 SV=1)

HSP 1 Score: 622 bits (1603), Expect = 8.37e-225
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60
           MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC
Sbjct: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60

Query: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120
           ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD
Sbjct: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120

Query: 121 PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180
           PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH
Sbjct: 121 PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180

Query: 181 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240
           VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG
Sbjct: 181 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240

Query: 241 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI 300
           KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI
Sbjct: 241 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI 300

Query: 301 NYTTAEDYMKRYL 313
           NYTTAEDYMKRYL
Sbjct: 301 NYTTAEDYMKRYL 313

BLAST of MC06g1710 vs. ExPASy TrEMBL
Match: A0A5A7VJ49 (Bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00120 PE=4 SV=1)

HSP 1 Score: 553 bits (1425), Expect = 1.13e-197
Identity = 273/311 (87.78%), Postives = 292/311 (93.89%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           KSKVLI+GGTGYLGKRLV+ASL LGHETYVLQR E+GV+IEK+ELLLSFKK+GAHL+C S
Sbjct: 4   KSKVLIIGGTGYLGKRLVKASLKLGHETYVLQRQEIGVDIEKIELLLSFKKKGAHLICGS 63

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
            NDHN+LVEA+KLVDVVISAISGVHIRSH ILLQL LV AIK+AGN+KRF+PSEFGTDPA
Sbjct: 64  LNDHNSLVEAIKLVDVVISAISGVHIRSHHILLQLNLVHAIKEAGNVKRFVPSEFGTDPA 123

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVL 182
           RME A EPGR TFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPG ILPSKDHVL
Sbjct: 124 RMEKAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGLILPSKDHVL 183

Query: 183 LLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 242
           LLGD NQKAIYVDEDDIAMYTMK+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ
Sbjct: 184 LLGDGNQKAIYVDEDDIAMYTMKSIDDSRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 243

Query: 243 LTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEINY 302
           LTKTSIS+ +FLA +KG+  YAEQVGLSHYY VCYEGCLANFEIGKDGEEACNLYPE+NY
Sbjct: 244 LTKTSISSQEFLANMKGQ-DYAEQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVNY 303

Query: 303 TTAEDYMKRYL 313
           TT E+YMKRYL
Sbjct: 304 TTVEEYMKRYL 313

BLAST of MC06g1710 vs. ExPASy TrEMBL
Match: A0A1S3CCK4 (bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucumis melo OX=3656 GN=LOC103499245 PE=4 SV=1)

HSP 1 Score: 553 bits (1425), Expect = 1.13e-197
Identity = 273/311 (87.78%), Postives = 292/311 (93.89%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           KSKVLI+GGTGYLGKRLV+ASL LGHETYVLQR E+GV+IEK+ELLLSFKK+GAHL+C S
Sbjct: 4   KSKVLIIGGTGYLGKRLVKASLKLGHETYVLQRQEIGVDIEKIELLLSFKKKGAHLICGS 63

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
            NDHN+LVEA+KLVDVVISAISGVHIRSH ILLQL LV AIK+AGN+KRF+PSEFGTDPA
Sbjct: 64  LNDHNSLVEAIKLVDVVISAISGVHIRSHHILLQLNLVHAIKEAGNVKRFVPSEFGTDPA 123

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVL 182
           RME A EPGR TFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPG ILPSKDHVL
Sbjct: 124 RMEKAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGLILPSKDHVL 183

Query: 183 LLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 242
           LLGD NQKAIYVDEDDIAMYTMK+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ
Sbjct: 184 LLGDGNQKAIYVDEDDIAMYTMKSIDDSRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 243

Query: 243 LTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEINY 302
           LTKTSIS+ +FLA +KG+  YAEQVGLSHYY VCYEGCLANFEIGKDGEEACNLYPE+NY
Sbjct: 244 LTKTSISSQEFLANMKGQ-DYAEQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVNY 303

Query: 303 TTAEDYMKRYL 313
           TT E+YMKRYL
Sbjct: 304 TTVEEYMKRYL 313

BLAST of MC06g1710 vs. ExPASy TrEMBL
Match: A0A0A0L8W5 (NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G636980 PE=4 SV=1)

HSP 1 Score: 543 bits (1400), Expect = 6.99e-194
Identity = 272/312 (87.18%), Postives = 292/312 (93.59%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVE-IEKVELLLSFKKQGAHLVCA 62
           KSKVLI+GGTGYLGKRLV+ASL LGHETYVLQR E+GV+ IEKVELLLSFKK+GAHL+C 
Sbjct: 2   KSKVLIIGGTGYLGKRLVKASLKLGHETYVLQRQEIGVDHIEKVELLLSFKKKGAHLICG 61

Query: 63  SFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDP 122
           SFNDHNTLV+A+KLVDVVIS+ISGVHIRSH ILLQL LV AIK+AGN+KRFLPSEFGTDP
Sbjct: 62  SFNDHNTLVKAIKLVDVVISSISGVHIRSHHILLQLNLVRAIKEAGNVKRFLPSEFGTDP 121

Query: 123 ARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHV 182
           ARME+A EPGR TFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHV
Sbjct: 122 ARMEDAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHV 181

Query: 183 LLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGK 242
           LLLGD NQKAIYVDEDDIAMYTMK+IDD RTLNKTVYIRPPKNILSQREVVEIWEKLIGK
Sbjct: 182 LLLGDGNQKAIYVDEDDIAMYTMKSIDDCRTLNKTVYIRPPKNILSQREVVEIWEKLIGK 241

Query: 243 QLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEIN 302
           QL KTSIS+ +FLA +K +  YA QVGLSHYY VCYEGCLANFEIGKDGEEACNLYPE++
Sbjct: 242 QLIKTSISSQEFLANMKDQ-DYAAQVGLSHYYHVCYEGCLANFEIGKDGEEACNLYPEVD 301

Query: 303 YTTAEDYMKRYL 313
           YTT E+YMKRYL
Sbjct: 302 YTTVEEYMKRYL 312

BLAST of MC06g1710 vs. ExPASy TrEMBL
Match: A0A6J1HGM2 (bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464117 PE=4 SV=1)

HSP 1 Score: 539 bits (1389), Expect = 3.32e-192
Identity = 268/313 (85.62%), Postives = 292/313 (93.29%), Query Frame = 0

Query: 1   MGKSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVC 60
           MGK+KVLI+GGTGYLGKRLVRASL LG++TYVLQR EMGV+IEKVELLLSFK++GAHLVC
Sbjct: 1   MGKNKVLIIGGTGYLGKRLVRASLRLGYQTYVLQRQEMGVDIEKVELLLSFKREGAHLVC 60

Query: 61  ASFNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTD 120
           ASFNDHN+LVEAVKLVDVVISAISGVHIRSH ILLQLKLV AIK+A N+KRFLPSEFGTD
Sbjct: 61  ASFNDHNSLVEAVKLVDVVISAISGVHIRSHHILLQLKLVHAIKEAPNVKRFLPSEFGTD 120

Query: 121 PARMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDH 180
           PARM+NA EPGR TFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPG ILPSK H
Sbjct: 121 PARMKNAMEPGRVTFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGFILPSKHH 180

Query: 181 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240
           V LLGD NQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG
Sbjct: 181 VSLLGDGNQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 240

Query: 241 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEI 300
           K+LTK+SIS  +FLA +KG+  YAEQVGL+HYY VC+EGCLANFEIG   EEAC+LYPE+
Sbjct: 241 KELTKSSISDQEFLANMKGQ-GYAEQVGLTHYYHVCFEGCLANFEIGDGAEEACSLYPEV 300

Query: 301 NYTTAEDYMKRYL 313
           +YT+ E+YMKRY+
Sbjct: 301 SYTSVEEYMKRYV 312

BLAST of MC06g1710 vs. TAIR 10
Match: AT1G32100.1 (pinoresinol reductase 1 )

HSP 1 Score: 389.4 bits (999), Expect = 2.7e-108
Identity = 190/311 (61.09%), Postives = 242/311 (77.81%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           K++VL+VG TGY+GKR+VRA L  GHETYVLQR E+G+EIEKV+L LSFKK GA +V  S
Sbjct: 9   KTRVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGS 68

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
           F+DH +LV AVKLVDVV+SA+SGVH RSH IL+QLKLV+AIK+AGN+KRFLPSEFG DP 
Sbjct: 69  FSDHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPP 128

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVL 182
           RM +A  PGR TFD KM VR+AIE A IP+TY+   CFA YF G L Q  ++LP K+ V 
Sbjct: 129 RMGHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVN 188

Query: 183 LLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 242
           + GD N K ++ DEDDIA YT KT++DPRTLNKTV IRPP N+L+Q E+V+IWEKL GK+
Sbjct: 189 IYGDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKE 248

Query: 243 LTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEINY 302
           L KT+I+A  FLA I+ ++    Q G+ H+Y + YEGCL + E+G+D EEA +LYP++ Y
Sbjct: 249 LEKTNIAAQDFLANIE-QMEIPHQAGIGHFYHIFYEGCLTDHEVGED-EEASSLYPDVKY 308

Query: 303 TTAEDYMKRYL 314
              +DY++ +L
Sbjct: 309 KRMDDYLRMFL 317

BLAST of MC06g1710 vs. TAIR 10
Match: AT4G13660.1 (pinoresinol reductase 2 )

HSP 1 Score: 366.7 bits (940), Expect = 1.9e-101
Identity = 185/312 (59.29%), Postives = 236/312 (75.64%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           K++VL+VGGTG LG+R+V A L  GHETYVLQR E+GV+IEKV+LLLSFK+ GAHLV  S
Sbjct: 9   KTRVLVVGGTGSLGRRIVSACLAEGHETYVLQRPEIGVDIEKVQLLLSFKRLGAHLVEGS 68

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
           F+DH +LV AVK VDVV+SA+SGVH R+H I +QLKLV AIK+AGN+KRFLPSEFG DP+
Sbjct: 69  FSDHQSLVSAVKQVDVVVSAMSGVHFRTHNIPVQLKLVAAIKEAGNVKRFLPSEFGMDPS 128

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVL 182
           RM +A  PG  TFD KM +R AI+ A I  TY+   CFA YF G L Q G++ P K+ V 
Sbjct: 129 RMGHAMPPGSETFDQKMEIRNAIKAAGISHTYLVGACFAAYFGGNLSQMGTLFPPKNKVD 188

Query: 183 LLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 242
           + GD N K ++VDEDD+A YT KT++DPRTLNKTVY+RP  NIL+Q E+V+IWEKL  K+
Sbjct: 189 IYGDGNVKVVFVDEDDMAKYTAKTLNDPRTLNKTVYVRPTDNILTQMELVQIWEKLTEKE 248

Query: 243 LTKTSISAHQFLAKIKG-EISYAEQVGLSHYYDVCYEGCLANFEIGKDGEEACNLYPEIN 302
           L KT +S + FLA I+  EIS+  Q GL H+Y + YEGCL + E+G D EEA  LYP++ 
Sbjct: 249 LEKTYVSGNDFLADIEDKEISH--QAGLGHFYHIYYEGCLTDHEVG-DDEEATKLYPDVK 308

Query: 303 YTTAEDYMKRYL 314
           Y   ++Y+K ++
Sbjct: 309 YKRMDEYLKIFV 317

BLAST of MC06g1710 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.5e-66
Identity = 143/313 (45.69%), Postives = 189/313 (60.38%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           KSK+L++GGTGY+GK LV AS   GH T+ L R     +  K + + SFK  G  ++   
Sbjct: 5   KSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGD 64

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
            NDH +LV+A+K VDVVIS +      S QIL Q K++ AIK+AGN+KRFLPSEFG D  
Sbjct: 65  LNDHESLVKAIKQVDVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGVDVD 124

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQ--PGSILPSKDH 182
           R  +A EP ++ F  K+ +R+ IE   IP+TY    CF GY+L  L Q  PG   P +D 
Sbjct: 125 R-TSAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDK 184

Query: 183 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 242
           V +LGD N KA+   E+DIA YT+K +DDPRTLNK +YI+P  N LS  E+V +WEK IG
Sbjct: 185 VTILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIG 244

Query: 243 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKD-GEEACNLYPE 302
           K L KT +   Q L  I+ E      V LS  + V   G   N  I    G EA  LYP+
Sbjct: 245 KSLEKTHLPEEQLLKSIQ-ESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPD 304

Query: 303 INYTTAEDYMKRY 313
           + YT+ ++Y+  +
Sbjct: 305 VKYTSVDEYLSYF 309

BLAST of MC06g1710 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.3e-65
Identity = 140/313 (44.73%), Postives = 190/313 (60.70%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           KSK+L++GGTG++GK ++ AS+  GH T  L R     +  K + + +FK  G  L+   
Sbjct: 5   KSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGD 64

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
            NDH +LV+A+K  DVVIS +      S QIL Q K++ AIK+AGN+KRFLPSEFG D  
Sbjct: 65  LNDHESLVKAIKQADVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGMDVD 124

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQ--PGSILPSKDH 182
           +  +A EP ++ F  K+  R+ IE   IP+TY+  N FAGY+L  L Q  PG   P +D 
Sbjct: 125 K-SSAVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDK 184

Query: 183 VLLLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIG 242
           V + GD N KA+   E+DIA YT+K +DDPRTLNKT+YI PP N LS  E+V +WEK IG
Sbjct: 185 VKIFGDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIG 244

Query: 243 KQLTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKD-GEEACNLYPE 302
           K + K  +S  Q    I+ E      V LS  + V  +G   NF I    G EA  LYP+
Sbjct: 245 KSVEKIYMSEEQIFKSIQ-ESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPD 304

Query: 303 INYTTAEDYMKRY 313
           I YT+ ++Y+  +
Sbjct: 305 IKYTSIDEYLSYF 310

BLAST of MC06g1710 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.2e-63
Identity = 133/312 (42.63%), Postives = 185/312 (59.29%), Query Frame = 0

Query: 3   KSKVLIVGGTGYLGKRLVRASLGLGHETYVLQRAEMGVEIEKVELLLSFKKQGAHLVCAS 62
           KSK+L +GGTGY+GK +V AS   GH T VL R        +   + +FK  G   +   
Sbjct: 4   KSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFLLGD 63

Query: 63  FNDHNTLVEAVKLVDVVISAISGVHIRSHQILLQLKLVDAIKQAGNIKRFLPSEFGTDPA 122
            +DH +LV ++K  DVVIS + G  +  H    Q K++ AIK+AGN+KRF PSEFG D  
Sbjct: 64  LDDHTSLVNSIKQADVVISTV-GHSLLGH----QYKIISAIKEAGNVKRFFPSEFGNDVD 123

Query: 123 RMENATEPGRATFDDKMVVRKAIEEAKIPFTYISANCFAGYFLGGLCQPGSILPSKDHVL 182
           R+    EP ++ +  K  +R+ IE   IP+TY+S N FAGYFL  L QPG+    +D V+
Sbjct: 124 RV-FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVI 183

Query: 183 LLGDANQKAIYVDEDDIAMYTMKTIDDPRTLNKTVYIRPPKNILSQREVVEIWEKLIGKQ 242
           +LGD N KA++  E+DI  YT+  +DDPRTLNK +YIRPP N  S  ++V +WE  IGK 
Sbjct: 184 VLGDGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKT 243

Query: 243 LTKTSISAHQFLAKIKGEISYAEQVGLSHYYDVCYEGCLANFEIGKD-GEEACNLYPEIN 302
           L +  +   Q L +I  E S    V LS  + V  +G   +FEI    G EA  LYP++ 
Sbjct: 244 LERIYVPEEQLLKQII-ESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVK 303

Query: 303 YTTAEDYMKRYL 314
           YTT ++ + +Y+
Sbjct: 304 YTTVDEILNQYV 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E6Y2X02.9e-13978.46Bifunctional pinoresinol-lariciresinol reductase 2 OS=Linum usitatissimum OX=400... [more]
B5KRH53.9e-13676.77Bifunctional pinoresinol-lariciresinol reductase OS=Linum corymbulosum OX=480358... [more]
Q4R0I03.3e-13574.68Bifunctional pinoresinol-lariciresinol reductase OS=Linum album OX=191219 GN=PLR... [more]
P931435.4e-13073.25Bifunctional pinoresinol-lariciresinol reductase OS=Forsythia intermedia OX=5518... [more]
B0LL232.1e-12973.16Bifunctional pinoresinol-lariciresinol reductase OS=Sinopodophyllum hexandrum OX... [more]
Match NameE-valueIdentityDescription
XP_022156026.11.73e-224100.00bifunctional pinoresinol-lariciresinol reductase 2-like [Momordica charantia][more]
XP_038888404.11.09e-20489.78bifunctional pinoresinol-lariciresinol reductase 2-like [Benincasa hispida][more]
XP_008460436.12.33e-19787.78PREDICTED: bifunctional pinoresinol-lariciresinol reductase 2-like [Cucumis melo... [more]
XP_011651690.11.44e-19387.18bifunctional pinoresinol-lariciresinol reductase 2 [Cucumis sativus] >KGN58400.1... [more]
XP_022963982.16.85e-19285.62bifunctional pinoresinol-lariciresinol reductase 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1DTL08.37e-225100.00bifunctional pinoresinol-lariciresinol reductase 2-like OS=Momordica charantia O... [more]
A0A5A7VJ491.13e-19787.78Bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucumis melo var. mak... [more]
A0A1S3CCK41.13e-19787.78bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucumis melo OX=3656 ... [more]
A0A0A0L8W56.99e-19487.18NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G636980 PE=4 S... [more]
A0A6J1HGM23.32e-19285.62bifunctional pinoresinol-lariciresinol reductase 2-like OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
AT1G32100.12.7e-10861.09pinoresinol reductase 1 [more]
AT4G13660.11.9e-10159.29pinoresinol reductase 2 [more]
AT1G75280.11.5e-6645.69NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.11.3e-6544.73NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G39230.11.2e-6342.63NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 6..261
e-value: 1.7E-64
score: 217.5
NoneNo IPR availableGENE3D3.90.25.10coord: 158..309
e-value: 5.6E-122
score: 408.5
NoneNo IPR availableGENE3D3.40.50.720coord: 4..298
e-value: 5.6E-122
score: 408.5
NoneNo IPR availablePANTHERPTHR43349:SF47BIFUNCTIONAL PINORESINOL-LARICIRESINOL REDUCTASE-LIKEcoord: 2..312
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 2..312
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 5..308
e-value: 2.43334E-70
score: 217.941
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 3..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1710.1MC06g1710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0016491 oxidoreductase activity