MC06g1306 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1306
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionvesicle-associated protein 1-2
LocationMC06: 19905238 .. 19909246 (+)
RNA-Seq ExpressionMC06g1306
SyntenyMC06g1306
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATAAAAGCCCAATAAAGGCCCGAAAGGCCCATCGAAAATGACCGATCGGCTAAATAAAGGAGAGAAGAGAGAAAGAGAGAGAGAGAGAGAGAGGAGTTACTTGGTTTGGTGCACGTGTTTTCGTGGAGCCTTCTCTTGTCTTCAGCACGTTCGCCATTGTTGCTCCTCTCTTTTCTCGATCTGTTCGCGAAACTTGCTCTCAACGAAACTCATTTCCAGATTCTCCTCTGCTCTTCCTCTAGTTTCTGTTCTGTATTCCTCAATTCTTCTTCCCTGTGTTCGCGGGCCACTGTTCCAACAATGAGTACGGGAGAACTCCTCAGCATCGAACCTCTCGAGCTCAAGTTTCCCTGTGAGTAGTTGTCGGGACGAAATTACTCGTTTCTAACACGCGGATCTGTAATCTTTTGGGGGTTTTTTTGCGTCTTTCAATTTTGTTCTCTCCTTACTTTCTTTTCTTTTTGCTGTTTCTTTGCGGTTAGTCGAATTGAAGAAGCAGATCTCTTGTTCCCTTCAATTGTCGAACAAGACCGAGAGCTACGTTGCATTTAAGGTACGATTCTTTTTAGTTCAGTTCAATTTTTATGATCTGGGTAATTGATCTTTTTCCAGTGAAATGCTTTAGTGTTCCTAAGAAGGTGTTTTGGATTTGTGATGTAGGCGTATGCAATTTTTGTTATTTCTTTTCCTTTTTCTCTTGATATATCTGTCTGGCGTCAGATCACGTTACGAAGTTTGTTAATTTCGTCAATTTTCAATTTGCTAACGGGGGGTTTTTTTTTTCAGTTTATAATTCTTTATTTACTGGCAAAATGCGCGCAGATTGAACTCGTGTTTGATCATTTTGTTCCATGTTTCTGTCCACTCAATATGAATTTTATCCTTTTCCAGGTTAAAACTACGAACCCTAAAAAGTACTGCGTTCGCCCAAACACTGGAATTGTCTCACCTCGGTCGACATGTGACGTTATAGGTGCCAAATGCCAATATCATATATTTCACTAATGGCTTTTAATTTCGAACAATTTTTGTGGTTACTTATTTTGGGAGCCCTTCTGTTGTGCAGTCACCATGCAAGCCCAGAAAGAGGCTCCTGCTGATATGCAATGCAAAGACAAGTTTCTCCTCCAGAGTGTTAAAACTGTTGATGGCGCATCCGCAAAGGAGATCAACGCAGAAATGGTATTTTATAGTGTGAATGTTTAAGGCTTGTGGTTCTTATTAGCCTTTTACTCTTTTTTATGGGCTTGGGATTTGCAGTTCAATAAGGAGGCAGGGCATGTGGTAGAAGAATGCAAATTGAAAGTCGTTTATGTTTCTCCACCTCAACCACCTTCTCCAGTACCGGAAGGTTCTGAAGAAGGGTCGTCCCCAAGAGGCTCTGTTTCAGAAAATGGAAACTTCAATGATACCGAGTTAAATACTGTGAGTAGTCAGATTCCCTTTTTTCTCTTTTCCCTTATTCATTCATTTTCCCAATTTGATTTACACGCCTTTACCTTCTGTTTGCAGCAGGCTACAAGGGGATATGTTGAACGGTTGGAGCCCCAGGATAAATCTGCAGAGGTAAAAGATAGGATGATTTTTTGCATGTTATAGGCATAAGGTTCTGTGCCTGCTGTTTATGATGATTCTCTATTTTGTGTTCTGTAACTCTGATGCCTGATTTATGTGTCTTTTATGATGTATTCGGTTTTTTCAGCCAAATTTCCACTTCTATGATAAAATTGTTATAAGATAGAACTCAATGCAAATATATGACCTTGTGGTTGGAGTGTTACTTTACTCTGAGCAGTAAACAATATAATTTTCCAAGCATTGTATTTTTAGTAATTGATGATATACTAGTCAAGATTTGGGTGAAAATGTTGCCTGTTGGAATTTCTTTTGTGGATTTCTCAAAATGATTGGCTATATTTCAAGGATCTTTTATTGTATTTCCAGCCTTACCTGAAAGATCATATTATGTGAAAGATGTTCTAGTGTGAACACAGACGTGAATGTAACTTCCTTGTCATAATATTCATAGTTGAGAATTTACTTTTGAGATTTTGAGTAAACTTGCTGGTTACTTGTGTTGTATAGATTTCTTGTAATGGTCTGGTCTCTTATATGATCTTTTATTGTATTTCCTAACTTTAAATACAACTATTATCATCAGAAAACAATGAACTTAGCATAATTCTGCTAGGGTTAATGAATTCATGGATATATGCTTTGGACATAATTTCTTGTCCACAAGTATTGCAATATTGGGATAGAAACACCATGGATTGGTCTAGTGGGGTGGTGAAGAGGTAAACTGTAAACCTCAATATTTTTGAGGTCATGAGTTCAAGAGCATCACCTACCCCAGTCAGTAAATATTTTACATTTTCCCAATGACAAGTAAAGAGTTAGGACAGGATGTCCACTTGGAGAGAACCTTCCCAAAACTGGTTGATTTAAAGGATCCTTTATCCATAAAGAAAGTGATGCTATATCCTTGTGGCTGTTTATTTATACTCTTTCCAAAGTTTCATAGCAACTAACTGATACTCTAAAGCATGTTTTATGTGTTCGGTTGGCTTGCTCGTTCAATGTTCTAATACTCCCACGAATACTCTATTCCTTGACATCACCATCCAACTTAATTTCCTTACACAGCTAATCCTAAGTCTCTTTGCCCAAAGATCCTAAGCTAGGCTTATGACGTGGTGACTATACCCCCCATTCAATTATAGAAAGAATGTGCTTCTATCTTCCAAGACATAATTTTTTCTGAGCCTCAAATCACAAGTGTGACTAGATACTAATTAGGTTAAAGATATTGGAGGTAATATGTGAAACTCCATCCGTTTTGCTTCAGAGCTCCTTGCCAAAATCTGAAAAGTACAATAATGTATAATTCCAACCACCTCTGGATCACTCGTTCTCTTGTTCCTTTAAATAAGAATATTGTTCTTTTCTTATTTGTTTGTGCAATGTTCTCAAACCTTTGTCATGGTTCTCAGATCTTTTTAAATTATTCGGCAGATGGTTTGATTGACTAAATCTTGGGCCACTTCAGCTATTTGTGTACGTGTGTTTTGTGTGTTTTGTGTGTTTTCGTATATGCATATGCCTTCTGCTTCTAGCAGGCCGCTACATACTTTTGTTGCAAGTTTATTGACCACCGATATATGTGCAGGCTAGAGCCCTCATAACTAAACTCACAGAAGAAAAGAATAATGCCATCCAACAGAACAACAAACTTCGTCAGGAATTGGTAAGAGGGATCGGTTTGAAGCTTCAGTAAATGCAATTTTGCACAAGAAGTTTCATGTTGCATTGGATTTTGGAAACATCACCTTTACTTTTGAGATTCTTCAAAAAATTTCTTGGTCTCTTTGCCTGAAACCTCTTGAAAGTTACTCTTAAAATATTTTCCATGTTGAATTTAGTTTGTGATTACTGATTACACTGTTGGGTTTGTCATGCTCAAATCTGATCCTTATTTTCATGGAATATTAGAATTATAATATCAGAGCTACATCAGTTTCTTATGTTGTGGAGGGTTTGCATTGTGCAGGAACTGTTGAAGCGTGAAGGCAGGAAAAACGCCGGTGGCATCTCGTTCCTGTTCGTCATATTAATCGGATTGATCGGAATAATCTTTGGCTATATTATTAAGAAGACTTGAGAATCTCCTTGCAGCAAAGTGGATTTACATCATTATATTCTCTGTGATGAAAGGAAGGGAACTACTAATGTTCAGTGGGTGGATCTGTAGTAAGTTTTTATTTGTTGAAGTAGCAAATTGTTAGGTGGTGTTCAAGAATTTGTAGTTGCTGAATTTGCAAGGTAGCACACTCCACACTCTCTGTAACTTAAAAGAGTTTTTTCTTGTTTTTTTCTTCCTTCTGCTTCTCTTACCATTCAACTTGTTATATTTTTCACTGTGGAACTGTTCAGTTAATAATTTTACATCCTCAATTGTTCAGCATTTTTTATTTTCCTTCCACTGTGCCATTAAATCAAAATACCAG

mRNA sequence

CAATAAAAGCCCAATAAAGGCCCGAAAGGCCCATCGAAAATGACCGATCGGCTAAATAAAGGAGAGAAGAGAGAAAGAGAGAGAGAGAGAGAGAGGAGTTACTTGGTTTGGTGCACGTGTTTTCGTGGAGCCTTCTCTTGTCTTCAGCACGTTCGCCATTGTTGCTCCTCTCTTTTCTCGATCTGTTCGCGAAACTTGCTCTCAACGAAACTCATTTCCAGATTCTCCTCTGCTCTTCCTCTAGTTTCTGTTCTGTATTCCTCAATTCTTCTTCCCTGTGTTCGCGGGCCACTGTTCCAACAATGAGTACGGGAGAACTCCTCAGCATCGAACCTCTCGAGCTCAAGTTTCCCTTCGAATTGAAGAAGCAGATCTCTTGTTCCCTTCAATTGTCGAACAAGACCGAGAGCTACGTTGCATTTAAGGTTAAAACTACGAACCCTAAAAAGTACTGCGTTCGCCCAAACACTGGAATTGTCTCACCTCGGTCGACATGTGACGTTATAGTCACCATGCAAGCCCAGAAAGAGGCTCCTGCTGATATGCAATGCAAAGACAAGTTTCTCCTCCAGAGTGTTAAAACTGTTGATGGCGCATCCGCAAAGGAGATCAACGCAGAAATGTTCAATAAGGAGGCAGGGCATGTGGTAGAAGAATGCAAATTGAAAGTCGTTTATGTTTCTCCACCTCAACCACCTTCTCCAGTACCGGAAGGTTCTGAAGAAGGGTCGTCCCCAAGAGGCTCTGTTTCAGAAAATGGAAACTTCAATGATACCGAGTTAAATACTGCTACAAGGGGATATGTTGAACGGTTGGAGCCCCAGGATAAATCTGCAGAGGCTAGAGCCCTCATAACTAAACTCACAGAAGAAAAGAATAATGCCATCCAACAGAACAACAAACTTCGTCAGGAATTGGAACTGTTGAAGCGTGAAGGCAGGAAAAACGCCGGTGGCATCTCGTTCCTGTTCGTCATATTAATCGGATTGATCGGAATAATCTTTGGCTATATTATTAAGAAGACTTGAGAATCTCCTTGCAGCAAAGTGGATTTACATCATTATATTCTCTGTGATGAAAGGAAGGGAACTACTAATGTTCAGTGGGTGGATCTGTAGTAAGTTTTTATTTGTTGAAGTAGCAAATTGTTAGGTGGTGTTCAAGAATTTGTAGTTGCTGAATTTGCAAGGTAGCACACTCCACACTCTCTGTAACTTAAAAGAGTTTTTTCTTGTTTTTTTCTTCCTTCTGCTTCTCTTACCATTCAACTTGTTATATTTTTCACTGTGGAACTGTTCAGTTAATAATTTTACATCCTCAATTGTTCAGCATTTTTTATTTTCCTTCCACTGTGCCATTAAATCAAAATACCAG

Coding sequence (CDS)

ATGAGTACGGGAGAACTCCTCAGCATCGAACCTCTCGAGCTCAAGTTTCCCTTCGAATTGAAGAAGCAGATCTCTTGTTCCCTTCAATTGTCGAACAAGACCGAGAGCTACGTTGCATTTAAGGTTAAAACTACGAACCCTAAAAAGTACTGCGTTCGCCCAAACACTGGAATTGTCTCACCTCGGTCGACATGTGACGTTATAGTCACCATGCAAGCCCAGAAAGAGGCTCCTGCTGATATGCAATGCAAAGACAAGTTTCTCCTCCAGAGTGTTAAAACTGTTGATGGCGCATCCGCAAAGGAGATCAACGCAGAAATGTTCAATAAGGAGGCAGGGCATGTGGTAGAAGAATGCAAATTGAAAGTCGTTTATGTTTCTCCACCTCAACCACCTTCTCCAGTACCGGAAGGTTCTGAAGAAGGGTCGTCCCCAAGAGGCTCTGTTTCAGAAAATGGAAACTTCAATGATACCGAGTTAAATACTGCTACAAGGGGATATGTTGAACGGTTGGAGCCCCAGGATAAATCTGCAGAGGCTAGAGCCCTCATAACTAAACTCACAGAAGAAAAGAATAATGCCATCCAACAGAACAACAAACTTCGTCAGGAATTGGAACTGTTGAAGCGTGAAGGCAGGAAAAACGCCGGTGGCATCTCGTTCCTGTTCGTCATATTAATCGGATTGATCGGAATAATCTTTGGCTATATTATTAAGAAGACTTGA

Protein sequence

MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVSPRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECKLKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEARALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKKT
Homology
BLAST of MC06g1306 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 7.0e-85
Identity = 162/240 (67.50%), Postives = 203/240 (84.58%), Query Frame = 0

Query: 3   TGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVSPR 62
           + ELL+I+P++L+FPFELKKQISCSL L NKT++YVAFKVKTTNPKKYCVRPNTG+V PR
Sbjct: 2   SNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPR 61

Query: 63  STCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECKLK 122
           S+ +V+VTMQAQKEAPAD+QCKDKFLLQ V    GA+ K++  EMF+KEAGH VEE KL+
Sbjct: 62  SSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKLR 121

Query: 123 VVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEARA 182
           VVYV+PP+PPSPV EGSEEGSSPR SVS+NGN +D     A R   +R++ QD S+EARA
Sbjct: 122 VVYVAPPRPPSPVREGSEEGSSPRASVSDNGNASD--FTAAPRFSADRVDAQDNSSEARA 181

Query: 183 LITKLTEEKNNAIQQNNKLRQELELLKREG-RKNAGGISFLFVILIGLIGIIFGYIIKKT 242
           L+TKLTEEKN+A+Q NN+L+QEL+ L+RE  R  +GGI F++V+L+GLIG+I GYI+K+T
Sbjct: 182 LVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMKRT 239

BLAST of MC06g1306 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 7.2e-82
Identity = 161/243 (66.26%), Postives = 203/243 (83.54%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MS  ELL++EPL+L+FPFELKKQISCSL L+NKT++ VAFKVKTTNPKKYCVRPNTG+V 
Sbjct: 17  MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVL 76

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTC+V+VTMQAQKEAP+DMQCKDKFLLQ V    G +AKE+  EMF+KEAGH VEE K
Sbjct: 77  PRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETK 136

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVE-RLEPQDKSAE 180
           L+V YV+PP+PPSPV EGSEEGSSPR SVS+NG+ ++       R  V+ +   Q+ ++E
Sbjct: 137 LRVTYVAPPRPPSPVHEGSEEGSSPRASVSDNGHGSEFSFE---RFIVDNKAGHQENTSE 196

Query: 181 ARALITKLTEEKNNAIQQNNKLRQELELLKREGRKN-AGGISFLFVILIGLIGIIFGYII 240
           ARALITKLTEEK +AIQ NN+L++EL+ L+RE +K+ +GGI F++V+L+GLIG+I GYI+
Sbjct: 197 ARALITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIM 256

Query: 241 KKT 242
           K+T
Sbjct: 257 KRT 256

BLAST of MC06g1306 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 2.4e-69
Identity = 141/242 (58.26%), Postives = 190/242 (78.51%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESY-VAFKVKTTNPKKYCVRPNTGIV 60
           M+TG+L++I P ELKFPFELKKQ SCS+QL+NKT +  VAFKVKTTNP+KYCVRPNTG+V
Sbjct: 1   MTTGDLVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVV 60

Query: 61  SPRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEEC 120
            P  +C+V VTMQAQKEAP DMQCKDKFL+Q+V   DG ++KE+ AEMFNKEAG V+E+ 
Sbjct: 61  LPGDSCNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDF 120

Query: 121 KLKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAE 180
           KL+VVY+ P  PPSPVPEGSEEG+SP  S+++  + + +  +  +R + E  E   KS+E
Sbjct: 121 KLRVVYI-PANPPSPVPEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSE---KSSE 180

Query: 181 ARALITKLTEEKNNAIQQNNKLRQELELLKRE-GRKNAGGISFLFVILIGLIGIIFGYII 240
           A ++I+KLTEEK +A QQ+ KLR ELE+L++E  +K +GG S L ++L+GL+G + GY++
Sbjct: 181 AWSMISKLTEEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLL 238

BLAST of MC06g1306 vs. ExPASy Swiss-Prot
Match: Q9SYC9 (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 3.9e-51
Identity = 99/153 (64.71%), Postives = 125/153 (81.70%), Query Frame = 0

Query: 5   ELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVSPRST 64
           ELL I+P++++FP EL K++SCSL L+NKTE+YVAFK KTTN KKY VRPN G+V PRS+
Sbjct: 175 ELLIIDPVDVQFPIELNKKVSCSLNLTNKTENYVAFKAKTTNAKKYYVRPNVGVVLPRSS 234

Query: 65  CDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECKLKVV 124
           C+V+V MQA KEAPADMQC+DK L Q        +AK++ +EMF+KEAGH  EE +LKV+
Sbjct: 235 CEVLVIMQALKEAPADMQCRDKLLFQCKVVEPETTAKDVTSEMFSKEAGHPAEETRLKVM 294

Query: 125 YVSPPQPPSPVPEGSEEGSSPRGSVSENGNFND 158
           YV+PPQPPSPV EG+EEGSSPR SVS+NGN ++
Sbjct: 295 YVTPPQPPSPVQEGTEEGSSPRASVSDNGNASE 327

BLAST of MC06g1306 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 3.3e-42
Identity = 104/239 (43.51%), Postives = 147/239 (61.51%), Query Frame = 0

Query: 5   ELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVSPRST 64
           +L+SI+P ELKF FEL+KQ  C L+++NKTE+YVAFKVKTT+PKKY VRPNTG++ P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 65  CDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECKLKVV 124
           C + VT+QAQ+E P DMQCKDKFLLQS          E+  + F K++G  + ECKLKV 
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 125 YVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEARALI 184
           Y++    PS     SE G                    AT G         +S+E  + I
Sbjct: 128 YIT----PSTTQRSSESG--------------------ATNG-------DGQSSETISTI 187

Query: 185 TKLTEEKNNAIQQNNKLRQELELL-KREGRKNAG-GISFLFVILIGLIGIIFGYIIKKT 242
            +L EE++ A++Q  +L+ ELE + +R  ++N+G G+S     ++GLIG+I G+I+K T
Sbjct: 188 QRLKEERDAAVKQTQQLQHELETVRRRRNQRNSGNGLSLKLAAMVGLIGLIIGFILKLT 215

BLAST of MC06g1306 vs. NCBI nr
Match: XP_022154809.1 (vesicle-associated protein 1-2 isoform X2 [Momordica charantia])

HSP 1 Score: 463 bits (1192), Expect = 2.98e-164
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS
Sbjct: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK
Sbjct: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180
           LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA
Sbjct: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180

Query: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240
           RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK
Sbjct: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240

BLAST of MC06g1306 vs. NCBI nr
Match: XP_022154808.1 (vesicle-associated protein 1-2 isoform X1 [Momordica charantia])

HSP 1 Score: 459 bits (1180), Expect = 2.08e-162
Identity = 241/242 (99.59%), Postives = 241/242 (99.59%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS
Sbjct: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK
Sbjct: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNT-ATRGYVERLEPQDKSAE 180
           LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNT ATRGYVERLEPQDKSAE
Sbjct: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTQATRGYVERLEPQDKSAE 180

Query: 181 ARALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIK 240
           ARALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIK
Sbjct: 181 ARALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIK 240

BLAST of MC06g1306 vs. NCBI nr
Match: XP_008437092.1 (PREDICTED: vesicle-associated protein 1-2 [Cucumis melo] >KAA0039712.1 vesicle-associated protein 1-2 [Cucumis melo var. makuwa])

HSP 1 Score: 431 bits (1107), Expect = 2.69e-151
Identity = 221/241 (91.70%), Postives = 232/241 (96.27%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKFPFELKKQISCSLQLSNKT+SYVAFKVKTTNPKKYCVRPNTGIVS
Sbjct: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTDSYVAFKVKTTNPKKYCVRPNTGIVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGA+AK+INAEMFNKEAGHVVEE K
Sbjct: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGATAKDINAEMFNKEAGHVVEEFK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180
           LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFND +LNTATRGYVER EPQDKSAEA
Sbjct: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDADLNTATRGYVERSEPQDKSAEA 180

Query: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240
           RALI KLTEEKNNA+QQ NKLRQELELLK + RKNA G+SF++VIL+GLIG+I GYIIKK
Sbjct: 181 RALIAKLTEEKNNAMQQYNKLRQELELLKHDSRKNASGVSFVYVILVGLIGVILGYIIKK 240

BLAST of MC06g1306 vs. NCBI nr
Match: XP_038874712.1 (vesicle-associated protein 1-2 [Benincasa hispida])

HSP 1 Score: 431 bits (1107), Expect = 2.69e-151
Identity = 223/241 (92.53%), Postives = 230/241 (95.44%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTES VAFKVKTTNPKKYCVRPNTGIVS
Sbjct: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESCVAFKVKTTNPKKYCVRPNTGIVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKT DGA+AK+I AEMFNKEAGHVVEE K
Sbjct: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDITAEMFNKEAGHVVEEFK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180
           LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFND +LNTATRGYVER EP DKSAEA
Sbjct: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDADLNTATRGYVERSEPLDKSAEA 180

Query: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240
           RALI KLTEEKNNA+QQNNKLRQELELLK + RKNAGG+SFLFVIL+GLIGI FGYIIKK
Sbjct: 181 RALIAKLTEEKNNALQQNNKLRQELELLKHDSRKNAGGVSFLFVILVGLIGIFFGYIIKK 240

BLAST of MC06g1306 vs. NCBI nr
Match: XP_022958657.1 (vesicle-associated protein 1-2-like [Cucurbita moschata] >XP_023533309.1 vesicle-associated protein 1-2-like [Cucurbita pepo subsp. pepo] >KAG6606179.1 Vesicle-associated protein 1-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 429 bits (1102), Expect = 1.56e-150
Identity = 220/241 (91.29%), Postives = 230/241 (95.44%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKF FELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTG+VS
Sbjct: 1   MSTGELLSIEPLELKFSFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           P STCDVIVTMQAQKEAPADMQCKDKFLLQSVKT DGA+AK+INAEMFNKEAGHVVEECK
Sbjct: 61  PGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180
           LKVVY  P QPPSPVPEGSEEGSSPRGS+SENGNFNDTELNTATRGYV+R EPQDKSAEA
Sbjct: 121 LKVVYCPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVDRSEPQDKSAEA 180

Query: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240
           RALI KLTEEKN+A+QQNNKLRQELELLKR+GRKN GG+SFL VILIGLIGI+ GYIIKK
Sbjct: 181 RALIAKLTEEKNSALQQNNKLRQELELLKRDGRKNGGGVSFLLVILIGLIGIMLGYIIKK 240

BLAST of MC06g1306 vs. ExPASy TrEMBL
Match: A0A6J1DN99 (vesicle-associated protein 1-2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021973 PE=4 SV=1)

HSP 1 Score: 463 bits (1192), Expect = 1.44e-164
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS
Sbjct: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK
Sbjct: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180
           LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA
Sbjct: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180

Query: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240
           RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK
Sbjct: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240

BLAST of MC06g1306 vs. ExPASy TrEMBL
Match: A0A6J1DLB2 (vesicle-associated protein 1-2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021973 PE=4 SV=1)

HSP 1 Score: 459 bits (1180), Expect = 1.01e-162
Identity = 241/242 (99.59%), Postives = 241/242 (99.59%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS
Sbjct: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK
Sbjct: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNT-ATRGYVERLEPQDKSAE 180
           LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNT ATRGYVERLEPQDKSAE
Sbjct: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTQATRGYVERLEPQDKSAE 180

Query: 181 ARALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIK 240
           ARALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIK
Sbjct: 181 ARALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIK 240

BLAST of MC06g1306 vs. ExPASy TrEMBL
Match: A0A5A7T8A8 (Vesicle-associated protein 1-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00430 PE=4 SV=1)

HSP 1 Score: 431 bits (1107), Expect = 1.30e-151
Identity = 221/241 (91.70%), Postives = 232/241 (96.27%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKFPFELKKQISCSLQLSNKT+SYVAFKVKTTNPKKYCVRPNTGIVS
Sbjct: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTDSYVAFKVKTTNPKKYCVRPNTGIVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGA+AK+INAEMFNKEAGHVVEE K
Sbjct: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGATAKDINAEMFNKEAGHVVEEFK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180
           LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFND +LNTATRGYVER EPQDKSAEA
Sbjct: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDADLNTATRGYVERSEPQDKSAEA 180

Query: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240
           RALI KLTEEKNNA+QQ NKLRQELELLK + RKNA G+SF++VIL+GLIG+I GYIIKK
Sbjct: 181 RALIAKLTEEKNNAMQQYNKLRQELELLKHDSRKNASGVSFVYVILVGLIGVILGYIIKK 240

BLAST of MC06g1306 vs. ExPASy TrEMBL
Match: A0A1S3ATQ2 (vesicle-associated protein 1-2 OS=Cucumis melo OX=3656 GN=LOC103482614 PE=4 SV=1)

HSP 1 Score: 431 bits (1107), Expect = 1.30e-151
Identity = 221/241 (91.70%), Postives = 232/241 (96.27%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKFPFELKKQISCSLQLSNKT+SYVAFKVKTTNPKKYCVRPNTGIVS
Sbjct: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTDSYVAFKVKTTNPKKYCVRPNTGIVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGA+AK+INAEMFNKEAGHVVEE K
Sbjct: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGATAKDINAEMFNKEAGHVVEEFK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180
           LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFND +LNTATRGYVER EPQDKSAEA
Sbjct: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDADLNTATRGYVERSEPQDKSAEA 180

Query: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240
           RALI KLTEEKNNA+QQ NKLRQELELLK + RKNA G+SF++VIL+GLIG+I GYIIKK
Sbjct: 181 RALIAKLTEEKNNAMQQYNKLRQELELLKHDSRKNASGVSFVYVILVGLIGVILGYIIKK 240

BLAST of MC06g1306 vs. ExPASy TrEMBL
Match: A0A6J1H2F2 (vesicle-associated protein 1-2-like OS=Cucurbita moschata OX=3662 GN=LOC111459814 PE=4 SV=1)

HSP 1 Score: 429 bits (1102), Expect = 7.53e-151
Identity = 220/241 (91.29%), Postives = 230/241 (95.44%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MSTGELLSIEPLELKF FELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTG+VS
Sbjct: 1   MSTGELLSIEPLELKFSFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVS 60

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           P STCDVIVTMQAQKEAPADMQCKDKFLLQSVKT DGA+AK+INAEMFNKEAGHVVEECK
Sbjct: 61  PGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECK 120

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEA 180
           LKVVY  P QPPSPVPEGSEEGSSPRGS+SENGNFNDTELNTATRGYV+R EPQDKSAEA
Sbjct: 121 LKVVYCPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVDRSEPQDKSAEA 180

Query: 181 RALITKLTEEKNNAIQQNNKLRQELELLKREGRKNAGGISFLFVILIGLIGIIFGYIIKK 240
           RALI KLTEEKN+A+QQNNKLRQELELLKR+GRKN GG+SFL VILIGLIGI+ GYIIKK
Sbjct: 181 RALIAKLTEEKNSALQQNNKLRQELELLKRDGRKNGGGVSFLLVILIGLIGIMLGYIIKK 240

BLAST of MC06g1306 vs. TAIR 10
Match: AT2G45140.1 (plant VAP homolog 12 )

HSP 1 Score: 315.1 bits (806), Expect = 5.0e-86
Identity = 162/240 (67.50%), Postives = 203/240 (84.58%), Query Frame = 0

Query: 3   TGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVSPR 62
           + ELL+I+P++L+FPFELKKQISCSL L NKT++YVAFKVKTTNPKKYCVRPNTG+V PR
Sbjct: 2   SNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPR 61

Query: 63  STCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECKLK 122
           S+ +V+VTMQAQKEAPAD+QCKDKFLLQ V    GA+ K++  EMF+KEAGH VEE KL+
Sbjct: 62  SSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKLR 121

Query: 123 VVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAEARA 182
           VVYV+PP+PPSPV EGSEEGSSPR SVS+NGN +D     A R   +R++ QD S+EARA
Sbjct: 122 VVYVAPPRPPSPVREGSEEGSSPRASVSDNGNASD--FTAAPRFSADRVDAQDNSSEARA 181

Query: 183 LITKLTEEKNNAIQQNNKLRQELELLKREG-RKNAGGISFLFVILIGLIGIIFGYIIKKT 242
           L+TKLTEEKN+A+Q NN+L+QEL+ L+RE  R  +GGI F++V+L+GLIG+I GYI+K+T
Sbjct: 182 LVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMKRT 239

BLAST of MC06g1306 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 305.1 bits (780), Expect = 5.1e-83
Identity = 161/243 (66.26%), Postives = 203/243 (83.54%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MS  ELL++EPL+L+FPFELKKQISCSL L+NKT++ VAFKVKTTNPKKYCVRPNTG+V 
Sbjct: 17  MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVL 76

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTC+V+VTMQAQKEAP+DMQCKDKFLLQ V    G +AKE+  EMF+KEAGH VEE K
Sbjct: 77  PRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETK 136

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVE-RLEPQDKSAE 180
           L+V YV+PP+PPSPV EGSEEGSSPR SVS+NG+ ++       R  V+ +   Q+ ++E
Sbjct: 137 LRVTYVAPPRPPSPVHEGSEEGSSPRASVSDNGHGSEFSFE---RFIVDNKAGHQENTSE 196

Query: 181 ARALITKLTEEKNNAIQQNNKLRQELELLKREGRKN-AGGISFLFVILIGLIGIIFGYII 240
           ARALITKLTEEK +AIQ NN+L++EL+ L+RE +K+ +GGI F++V+L+GLIG+I GYI+
Sbjct: 197 ARALITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIM 256

Query: 241 KKT 242
           K+T
Sbjct: 257 KRT 256

BLAST of MC06g1306 vs. TAIR 10
Match: AT4G00170.1 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 263.5 bits (672), Expect = 1.7e-70
Identity = 141/242 (58.26%), Postives = 190/242 (78.51%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESY-VAFKVKTTNPKKYCVRPNTGIV 60
           M+TG+L++I P ELKFPFELKKQ SCS+QL+NKT +  VAFKVKTTNP+KYCVRPNTG+V
Sbjct: 1   MTTGDLVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVV 60

Query: 61  SPRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEEC 120
            P  +C+V VTMQAQKEAP DMQCKDKFL+Q+V   DG ++KE+ AEMFNKEAG V+E+ 
Sbjct: 61  LPGDSCNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDF 120

Query: 121 KLKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGNFNDTELNTATRGYVERLEPQDKSAE 180
           KL+VVY+ P  PPSPVPEGSEEG+SP  S+++  + + +  +  +R + E  E   KS+E
Sbjct: 121 KLRVVYI-PANPPSPVPEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSE---KSSE 180

Query: 181 ARALITKLTEEKNNAIQQNNKLRQELELLKRE-GRKNAGGISFLFVILIGLIGIIFGYII 240
           A ++I+KLTEEK +A QQ+ KLR ELE+L++E  +K +GG S L ++L+GL+G + GY++
Sbjct: 181 AWSMISKLTEEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLL 238

BLAST of MC06g1306 vs. TAIR 10
Match: AT3G60600.2 (vesicle associated protein )

HSP 1 Score: 237.3 bits (604), Expect = 1.3e-62
Identity = 117/154 (75.97%), Postives = 136/154 (88.31%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MS  ELL++EPL+L+FPFELKKQISCSL L+NKT++ VAFKVKTTNPKKYCVRPNTG+V 
Sbjct: 17  MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVL 76

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTC+V+VTMQAQKEAP+DMQCKDKFLLQ V    G +AKE+  EMF+KEAGH VEE K
Sbjct: 77  PRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETK 136

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENGN 155
           L+V YV+PP+PPSPV EGSEEGSSPR SVS+NG+
Sbjct: 137 LRVTYVAPPRPPSPVHEGSEEGSSPRASVSDNGH 170

BLAST of MC06g1306 vs. TAIR 10
Match: AT3G60600.3 (vesicle associated protein )

HSP 1 Score: 236.9 bits (603), Expect = 1.7e-62
Identity = 122/175 (69.71%), Postives = 143/175 (81.71%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFPFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGIVS 60
           MS  ELL++EPL+L+FPFELKKQISCSL L+NKT++ VAFKVKTTNPKKYCVRPNTG+V 
Sbjct: 17  MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVL 76

Query: 61  PRSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTVDGASAKEINAEMFNKEAGHVVEECK 120
           PRSTC+V+VTMQAQKEAP+DMQCKDKFLLQ V    G +AKE+  EMF+KEAGH VEE K
Sbjct: 77  PRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETK 136

Query: 121 LKVVYVSPPQPPSPVPEGSEEGSSPRGSVSENG----------NFNDTELNTATR 166
           L+V YV+PP+PPSPV EGSEEGSSPR SVS+NG          NF+  +L+  TR
Sbjct: 137 LRVTYVAPPRPPSPVHEGSEEGSSPRASVSDNGHGSEFSIFMFNFSLRDLSWTTR 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SHC87.0e-8567.50Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q8VZ957.2e-8266.26Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q84WW52.4e-6958.26Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q9SYC93.9e-5164.71Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=... [more]
Q9LVU13.3e-4243.51Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_022154809.12.98e-164100.00vesicle-associated protein 1-2 isoform X2 [Momordica charantia][more]
XP_022154808.12.08e-16299.59vesicle-associated protein 1-2 isoform X1 [Momordica charantia][more]
XP_008437092.12.69e-15191.70PREDICTED: vesicle-associated protein 1-2 [Cucumis melo] >KAA0039712.1 vesicle-a... [more]
XP_038874712.12.69e-15192.53vesicle-associated protein 1-2 [Benincasa hispida][more]
XP_022958657.11.56e-15091.29vesicle-associated protein 1-2-like [Cucurbita moschata] >XP_023533309.1 vesicle... [more]
Match NameE-valueIdentityDescription
A0A6J1DN991.44e-164100.00vesicle-associated protein 1-2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1DLB21.01e-16299.59vesicle-associated protein 1-2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A5A7T8A81.30e-15191.70Vesicle-associated protein 1-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A1S3ATQ21.30e-15191.70vesicle-associated protein 1-2 OS=Cucumis melo OX=3656 GN=LOC103482614 PE=4 SV=1[more]
A0A6J1H2F27.53e-15191.29vesicle-associated protein 1-2-like OS=Cucurbita moschata OX=3662 GN=LOC11145981... [more]
Match NameE-valueIdentityDescription
AT2G45140.15.0e-8667.50plant VAP homolog 12 [more]
AT3G60600.15.1e-8366.26vesicle associated protein [more]
AT4G00170.11.7e-7058.26Plant VAMP (vesicle-associated membrane protein) family protein [more]
AT3G60600.21.3e-6275.97vesicle associated protein [more]
AT3G60600.31.7e-6269.71vesicle associated protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 177..211
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..163
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..174
NoneNo IPR availablePANTHERPTHR10809:SF130VESICLE-ASSOCIATED PROTEIN 1-2-LIKEcoord: 1..239
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 1..240
e-value: 3.1E-67
score: 225.4
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..239
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 7..110
e-value: 2.4E-31
score: 107.8
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 6..126
score: 30.383535
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 2..130
e-value: 2.2E-46
score: 158.9
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 2..141

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1306.1MC06g1306.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane