MC06g0540 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g0540
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Hydroxymethylbilane hydrolyase [cyclizing]
Location	MC06: 4446284 .. 4447204 (+)
RNA-Seq Expression	MC06g0540
Synteny	MC06g0540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCACCAGCGCCGGCCGTCCCGGCCCAATCCGCCAAAATCCACTCGGTTCATCTCCTTCTTCCGCCATTCCCGGCCACTCCATTCGGCGTACGGCGGCTTTCACGACGCCGCAGAACTACGCCGGCAGCCTCTCCCGCCTCCTCACTCTCAAAGGCATGGATCCCCTGTGGTGCCCCACCCTCACCGTCCAGCCCACTCCCCACGCCATCAAATCCCACATCCTTCCCCCAAATCTCGAATCCTTCTCCGCCGTCGCTTTCACTTCCCGCGCCGGAATTTCAGCTCTCCTCGACGCTGCAACTGAAATCGGCGAACCCTTGCTACCGTCGCAGGGCGAGACTTTTCTAATCGCTGCCCTAGGTAAGGATTCGGAGCTTCTCGACCATGGATTTATTTCCCAAATTTGCCCTAACGCGAGTAGAATTCAAGTCGTGCTCCCTAAAATAGCAACTCCGAGTGGTTTAGTGGAGGCTCTTGGAGTTGGAAACCGCCGGCGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGGAGGAGCCTCCGGTAGTTCCGAACTTCCTCCACGACCTGGAGGCGAACGGATGGGTTCCGGTTCGGGTCGATGCGTACGAGACCCGATGGGCCGGACCCGGGTGCGCGAGGCAGCTCGTGGAGAGAGATGAGAAATTGGATGCCATTGTGTTCACAAGTACTGGGGAAGTGGAGGGCCTGCTGAAAAGCTTGAGGGATTTAGGGTTCGAGTGGGAGACGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCCGGAGCCGAGAGGCTTGGCGTTAAGGTTGATTTGGTGAGTTGTAGATTCGATAGCTTTAACGGTGTGGTTGATGCTCTTCATTCCAGATGGCAGAGCTTAGAACAGAACCCT

mRNA sequence

ATGAGCACCAGCGCCGGCCGTCCCGGCCCAATCCGCCAAAATCCACTCGGTTCATCTCCTTCTTCCGCCATTCCCGGCCACTCCATTCGGCGTACGGCGGCTTTCACGACGCCGCAGAACTACGCCGGCAGCCTCTCCCGCCTCCTCACTCTCAAAGGCATGGATCCCCTGTGGTGCCCCACCCTCACCGTCCAGCCCACTCCCCACGCCATCAAATCCCACATCCTTCCCCCAAATCTCGAATCCTTCTCCGCCGTCGCTTTCACTTCCCGCGCCGGAATTTCAGCTCTCCTCGACGCTGCAACTGAAATCGGCGAACCCTTGCTACCGTCGCAGGGCGAGACTTTTCTAATCGCTGCCCTAGGTAAGGATTCGGAGCTTCTCGACCATGGATTTATTTCCCAAATTTGCCCTAACGCGAGTAGAATTCAAGTCGTGCTCCCTAAAATAGCAACTCCGAGTGGTTTAGTGGAGGCTCTTGGAGTTGGAAACCGCCGGCGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGGAGGAGCCTCCGGTAGTTCCGAACTTCCTCCACGACCTGGAGGCGAACGGATGGGTTCCGGTTCGGGTCGATGCGTACGAGACCCGATGGGCCGGACCCGGGTGCGCGAGGCAGCTCGTGGAGAGAGATGAGAAATTGGATGCCATTGTGTTCACAAGTACTGGGGAAGTGGAGGGCCTGCTGAAAAGCTTGAGGGATTTAGGGTTCGAGTGGGAGACGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCCGGAGCCGAGAGGCTTGGCGTTAAGGTTGATTTGGTGAGTTGTAGATTCGATAGCTTTAACGGTGTGGTTGATGCTCTTCATTCCAGATGGCAGAGCTTAGAACAGAACCCT

Coding sequence (CDS)

ATGAGCACCAGCGCCGGCCGTCCCGGCCCAATCCGCCAAAATCCACTCGGTTCATCTCCTTCTTCCGCCATTCCCGGCCACTCCATTCGGCGTACGGCGGCTTTCACGACGCCGCAGAACTACGCCGGCAGCCTCTCCCGCCTCCTCACTCTCAAAGGCATGGATCCCCTGTGGTGCCCCACCCTCACCGTCCAGCCCACTCCCCACGCCATCAAATCCCACATCCTTCCCCCAAATCTCGAATCCTTCTCCGCCGTCGCTTTCACTTCCCGCGCCGGAATTTCAGCTCTCCTCGACGCTGCAACTGAAATCGGCGAACCCTTGCTACCGTCGCAGGGCGAGACTTTTCTAATCGCTGCCCTAGGTAAGGATTCGGAGCTTCTCGACCATGGATTTATTTCCCAAATTTGCCCTAACGCGAGTAGAATTCAAGTCGTGCTCCCTAAAATAGCAACTCCGAGTGGTTTAGTGGAGGCTCTTGGAGTTGGAAACCGCCGGCGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGGAGGAGCCTCCGGTAGTTCCGAACTTCCTCCACGACCTGGAGGCGAACGGATGGGTTCCGGTTCGGGTCGATGCGTACGAGACCCGATGGGCCGGACCCGGGTGCGCGAGGCAGCTCGTGGAGAGAGATGAGAAATTGGATGCCATTGTGTTCACAAGTACTGGGGAAGTGGAGGGCCTGCTGAAAAGCTTGAGGGATTTAGGGTTCGAGTGGGAGACGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCCGGAGCCGAGAGGCTTGGCGTTAAGGTTGATTTGGTGAGTTGTAGATTCGATAGCTTTAACGGTGTGGTTGATGCTCTTCATTCCAGATGGCAGAGCTTAGAACAGAACCCT

Protein sequence

MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCPTLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEEPPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRWQSLEQNP

Homology

[Close]

BLAST of MC06g0540 vs. NCBI nr
Match: XP_022134809.1 (uncharacterized protein LOC111006990 [Momordica charantia])

HSP 1 Score: 617 bits (1592), Expect = 5.34e-223
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60
           MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP
Sbjct: 1   MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60

Query: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA 120
           TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA
Sbjct: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA 120

Query: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE 180
           LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE
Sbjct: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE 180

Query: 181 PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK 240
           PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK
Sbjct: 181 PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK 240

Query: 241 SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW 300
           SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW
Sbjct: 241 SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW 300

Query: 301 QSLEQNP 307
           QSLEQNP
Sbjct: 301 QSLEQNP 307

[Close]

BLAST of MC06g0540 vs. NCBI nr
Match: XP_023003818.1 (uncharacterized protein LOC111497286 [Cucurbita maxima])

HSP 1 Score: 503 bits (1294), Expect = 1.48e-177
Identity = 251/309 (81.23%), Postives = 272/309 (88.03%), Query Frame = 0


Query: 1   MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60
           MST A  P  I  NPL SSPS AIP +S  RT AFTTPQNYAGSLS LL+LKG DPLWCP
Sbjct: 1   MSTGAAHPSLIHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWCP 60

Query: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA 120
           T+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI +PLL  QG+TFLIAA
Sbjct: 61  TVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDDPLLSPQGDTFLIAA 120

Query: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE 180
           LGKDSELLDHG +S+ CPN SRI+VV+PKIATPSGLVEALG+GN RRVLCPVPRVVGL E
Sbjct: 121 LGKDSELLDHGVLSKFCPNTSRIRVVVPKIATPSGLVEALGIGNHRRVLCPVPRVVGLNE 180

Query: 181 PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEGL 240
           PPVVPNFL DL A+GWVPVRVDAYETRWAGP CAR LV+R  DEKLDAIVFTSTGEVEGL
Sbjct: 181 PPVVPNFLRDLAASGWVPVRVDAYETRWAGPECARMLVKRGEDEKLDAIVFTSTGEVEGL 240

Query: 241 LKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHS 300
           LKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALHS
Sbjct: 241 LKSLRTLGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALHS 300

Query: 301 RWQSLEQNP 307
           RWQSLEQNP
Sbjct: 301 RWQSLEQNP 309

[Close]

BLAST of MC06g0540 vs. NCBI nr
Match: KAG6587847.1 (hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035661.1 hypothetical protein SDJN02_02459, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 502 bits (1293), Expect = 2.18e-177
Identity = 252/310 (81.29%), Postives = 273/310 (88.06%), Query Frame = 0


Query: 1   MSTSAGRPGPIRQ-NPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MST A  P PI   NPL SSPS AIP +S  RT AFTTPQNYAGSLS LL+LKG DPLWC
Sbjct: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIA 120
           PT+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI EPLL  QG+TFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLE 180
           ALGKDSELLDHG +S+ CPNASRI+VV+PKIA+PSGLVEALG+GN RRVLCPVPRVVGL 
Sbjct: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFL DL A+GWVPVRVDAYETRW GP CAR+L ER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 307
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

[Close]

BLAST of MC06g0540 vs. NCBI nr
Match: XP_023530963.1 (uncharacterized protein LOC111793350 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500 bits (1288), Expect = 1.26e-176
Identity = 250/310 (80.65%), Postives = 273/310 (88.06%), Query Frame = 0


Query: 1   MSTSAGRPGPIRQ-NPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MST A  P PI   NPL SSPS AIP +S  RT AFTTPQNYAGSLS LL+LKG DPLWC
Sbjct: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIA 120
           PT+TV PTP AIKSH++PPNL  +SAVAFTSR+GI+ALLDAATEI EPLL  QG+TFLIA
Sbjct: 61  PTVTVHPTPIAIKSHLVPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLE 180
           ALGKDSELLDHG +S+ CPNA+RI+VV+PKIA+PSGLVEALG+GN RRVLCPVPRVVGL 
Sbjct: 121 ALGKDSELLDHGVLSKFCPNANRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFL DL A+GWVPVRVDAYETRW GP CAR+L ER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALH
Sbjct: 241 LLKSLRALGLQWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 307
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

[Close]

BLAST of MC06g0540 vs. NCBI nr
Match: XP_022926996.1 (uncharacterized protein LOC111433956 [Cucurbita moschata])

HSP 1 Score: 500 bits (1288), Expect = 1.26e-176
Identity = 252/310 (81.29%), Postives = 272/310 (87.74%), Query Frame = 0


Query: 1   MSTSAGRPGPIRQ-NPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MST A  P PIR  NPL SSPS AI  +S  RT AFTTPQNYAGSLS LL+LKG DPLWC
Sbjct: 1   MSTGAAHPSPIRHLNPLASSPSPAISTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIA 120
           PT+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI EPLL  QG+TFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLE 180
           ALGKDSELLDHG  S+ CPNASRI+VV+PKIA+PSGLVEALG+GN RRVLCPVPRVVGL 
Sbjct: 121 ALGKDSELLDHGVFSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFL DL A+GWVPVRVDAYETRW GP CAR+L ER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 307
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

[Close]

BLAST of MC06g0540 vs. ExPASy TrEMBL
Match: A0A6J1BZU3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Momordica charantia OX=3673 GN=LOC111006990 PE=3 SV=1)

HSP 1 Score: 617 bits (1592), Expect = 2.59e-223
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 0


Query: 1   MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60
           MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP
Sbjct: 1   MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60

Query: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA 120
           TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA
Sbjct: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA 120

Query: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE 180
           LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE
Sbjct: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE 180

Query: 181 PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK 240
           PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK
Sbjct: 181 PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK 240

Query: 241 SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW 300
           SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW
Sbjct: 241 SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW 300

Query: 301 QSLEQNP 307
           QSLEQNP
Sbjct: 301 QSLEQNP 307

[Close]

BLAST of MC06g0540 vs. ExPASy TrEMBL
Match: A0A6J1KXQ2 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita maxima OX=3661 GN=LOC111497286 PE=3 SV=1)

HSP 1 Score: 503 bits (1294), Expect = 7.18e-178
Identity = 251/309 (81.23%), Postives = 272/309 (88.03%), Query Frame = 0


Query: 1   MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60
           MST A  P  I  NPL SSPS AIP +S  RT AFTTPQNYAGSLS LL+LKG DPLWCP
Sbjct: 1   MSTGAAHPSLIHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWCP 60

Query: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA 120
           T+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI +PLL  QG+TFLIAA
Sbjct: 61  TVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDDPLLSPQGDTFLIAA 120

Query: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE 180
           LGKDSELLDHG +S+ CPN SRI+VV+PKIATPSGLVEALG+GN RRVLCPVPRVVGL E
Sbjct: 121 LGKDSELLDHGVLSKFCPNTSRIRVVVPKIATPSGLVEALGIGNHRRVLCPVPRVVGLNE 180

Query: 181 PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEGL 240
           PPVVPNFL DL A+GWVPVRVDAYETRWAGP CAR LV+R  DEKLDAIVFTSTGEVEGL
Sbjct: 181 PPVVPNFLRDLAASGWVPVRVDAYETRWAGPECARMLVKRGEDEKLDAIVFTSTGEVEGL 240

Query: 241 LKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHS 300
           LKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALHS
Sbjct: 241 LKSLRTLGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALHS 300

Query: 301 RWQSLEQNP 307
           RWQSLEQNP
Sbjct: 301 RWQSLEQNP 309

[Close]

BLAST of MC06g0540 vs. ExPASy TrEMBL
Match: A0A6J1EGG3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC111433956 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 6.10e-177
Identity = 252/310 (81.29%), Postives = 272/310 (87.74%), Query Frame = 0


Query: 1   MSTSAGRPGPIRQ-NPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MST A  P PIR  NPL SSPS AI  +S  RT AFTTPQNYAGSLS LL+LKG DPLWC
Sbjct: 1   MSTGAAHPSPIRHLNPLASSPSPAISTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIA 120
           PT+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI EPLL  QG+TFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLE 180
           ALGKDSELLDHG  S+ CPNASRI+VV+PKIA+PSGLVEALG+GN RRVLCPVPRVVGL 
Sbjct: 121 ALGKDSELLDHGVFSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFL DL A+GWVPVRVDAYETRW GP CAR+L ER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 307
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

[Close]

BLAST of MC06g0540 vs. ExPASy TrEMBL
Match: A0A5D3BX13 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00630 PE=3 SV=1)

HSP 1 Score: 499 bits (1285), Expect = 1.51e-176
Identity = 253/307 (82.41%), Postives = 272/307 (88.60%), Query Frame = 0


Query: 1   MSTSAGRP-GPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MST A  P GP   +PL SSPS AIP HS  RT AFTTPQNYAGSLS LL+LKG +PLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIA 120
           PTLTVQPTP AIKSH+LPP L SFSAVAFTSR+GI+ALLDAATEIGEPLLPS G+TFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLE 180
           ALGKDSELLDH F++ ICPN SRI+VV+P+IATP+GLVEALGVGN RRVLCPVPRVVGL 
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFL DLEA GWVPVRVDAYETRWAGP CAR+LVER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSL  LG EW+ M+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS +FDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 304
            RWQSL+
Sbjct: 301 WRWQSLD 307

[Close]

BLAST of MC06g0540 vs. ExPASy TrEMBL
Match: A0A5A7U8V3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00050 PE=3 SV=1)

HSP 1 Score: 499 bits (1285), Expect = 1.51e-176
Identity = 253/307 (82.41%), Postives = 272/307 (88.60%), Query Frame = 0


Query: 1   MSTSAGRP-GPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MST A  P GP   +PL SSPS AIP HS  RT AFTTPQNYAGSLS LL+LKG +PLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIA 120
           PTLTVQPTP AIKSH+LPP L SFSAVAFTSR+GI+ALLDAATEIGEPLLPS G+TFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLE 180
           ALGKDSELLDH F++ ICPN SRI+VV+P+IATP+GLVEALGVGN RRVLCPVPRVVGL 
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFL DLEA GWVPVRVDAYETRWAGP CAR+LVER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSL  LG EW+ M+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS +FDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 304
            RWQSL+
Sbjct: 301 WRWQSLD 307

The following BLAST results are available for this feature:

BLAST of MC06g0540 vs.


Match Name	E-value	Identity	Description

BLAST of MC06g0540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134809.1	5.34e-223	100.00	uncharacterized protein LOC111006990 [Momordica charantia]	[more]
XP_023003818.1	1.48e-177	81.23	uncharacterized protein LOC111497286 [Cucurbita maxima]	[more]
KAG6587847.1	2.18e-177	81.29	hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023530963.1	1.26e-176	80.65	uncharacterized protein LOC111793350 [Cucurbita pepo subsp. pepo]	[more]
XP_022926996.1	1.26e-176	81.29	uncharacterized protein LOC111433956 [Cucurbita moschata]	[more]

BLAST of MC06g0540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZU3	2.59e-223	100.00	Hydroxymethylbilane hydrolyase [cyclizing] OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1KXQ2	7.18e-178	81.23	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1EGG3	6.10e-177	81.29	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A5D3BX13	1.51e-176	82.41	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A5A7U8V3	1.51e-176	82.41	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of MC06g0540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	PFAM	PF02602	HEM4	coord: 45..289 e-value: 7.3E-24 score: 84.4
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	CDD	cd06578	HemD	coord: 36..296 e-value: 2.87537E-26 score: 102.0
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	GENE3D	3.40.50.10090		coord: 185..302 e-value: 3.0E-13 score: 51.6
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	SUPERFAMILY	69618	HemD-like	coord: 33..299
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR38020	UROPORPHYRINOGEN-III SYNTHASE	coord: 27..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g0540.1	MC06g0540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0033014	tetrapyrrole biosynthetic process
molecular_function	GO:0004852	uroporphyrinogen-III synthase activity