MC05g_new0400 (gene) Bitter gourd (Dali-11) v1

BLAST of MC05g_new0400 vs. ExPASy Swiss-Prot
Match: Q9ZVA5 (EP1-like glycoprotein 4 OS=Arabidopsis thaliana OX=3702 GN=At1g78860 PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 4.8e-20
Identity = 85/318 (26.73%), Postives = 137/318 (43.08%), Query Frame = 0

Query: 169 VWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALV 228
           VW +   S     E   L    DG+L L    G V W+T T+ +GV  ++IL  GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRVVWQTNTANKGVVGIKILENGNMVIY 149

Query: 229 DALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANANATSFYSFEIEPQRLALYI 288
           D+     WQSF+ PTD +L+GQSL  N       +  + + NA   YS  +E ++L LY 
Sbjct: 150 DSNGKFVWQSFDSPTDTLLVGQSLKLNGQ-NKLVSRLSPSVNANGPYSLVMEAKKLVLYY 209

Query: 289 NSNSAKSKYSYWEFNPPNNI-NLSFIT------------LNTQGLDIFSDQAKKIATIPS 348
            +N       Y+E+     I  L  +T            L+ +G+D  S     ++T  S
Sbjct: 210 TTNKTPKPIGYYEYEFFTKIAQLQSMTFQAVEDADTTWGLHMEGVD--SGSQFNVSTFLS 269

Query: 349 QSEQ--PLRFLALGNTTGNLGLYSYS--PQKGIFEASFRAL-------KTTCDLPLACKP 408
           + +    L FL L  + GN+ ++SYS       ++ ++ A           C +P  C  
Sbjct: 270 RPKHNATLSFLRL-ESDGNIRVWSYSTLATSTAWDVTYTAFTNDNTDGNDECRIPEHCLG 329

Query: 409 YGICTFSNSCSCIRVEMG----NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 459
           +G+C     C+    ++G    +  C   +I S   C        ++EG  S + +    
Sbjct: 330 FGLCK-KGQCNACPSDIGLLGWDETC---KIPSLASCDPKTFHYFKIEGADSFMTKYNGG 389

BLAST of MC05g_new0400 vs. ExPASy Swiss-Prot
Match: Q9ZVA4 (EP1-like glycoprotein 3 OS=Arabidopsis thaliana OX=3702 GN=At1g78850 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 3.1e-19
Identity = 79/316 (25.00%), Postives = 136/316 (43.04%), Query Frame = 0

Query: 169 VWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALV 228
           VW +   S     E   L    DG+L L    G + W+T T+ +G   ++IL  GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRLVWQTNTANKGAVGIKILENGNMVIY 149

Query: 229 DALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANANATSFYSFEIEPQRLALYI 288
           D+     WQSF+ PTD +L+GQSL  N   T   +  + + N    YS  +E ++L LY 
Sbjct: 150 DSSGKFVWQSFDSPTDTLLVGQSLKLNGR-TKLVSRLSPSVNTNGPYSLVMEAKKLVLYY 209

Query: 289 NSNSAKSKYSYWEFNPPNNINLSFITLNTQGLD------------IFSDQAKKIATIPSQ 348
            +N      +Y+E+     I   F ++  Q ++            + S     ++T  S+
Sbjct: 210 TTNKTPKPIAYFEYEFFTKIT-QFQSMTFQAVEDSDTTWGLVMEGVDSGSKFNVSTFLSR 269

Query: 349 SEQ--PLRFLALGNTTGNLGLYSYS--PQKGIFEASFRALKTT-------CDLPLACKPY 408
            +    L F+ L  + GN+ ++SYS       ++ ++ A           C +P  C  +
Sbjct: 270 PKHNATLSFIRL-ESDGNIRVWSYSTLATSTAWDVTYTAFTNADTDGNDECRIPEHCLGF 329

Query: 409 GICTFS--NSCSCIRVEMG-NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN 459
           G+C     N+C   +  +G +  C    + S   C        ++EG  S + +     +
Sbjct: 330 GLCKKGQCNACPSDKGLLGWDETCKSPSLAS---CDPKTFHYFKIEGADSFMTKYNGGSS 389

BLAST of MC05g_new0400 vs. ExPASy Swiss-Prot
Match: Q9ZVA2 (EP1-like glycoprotein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g78830 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 4.0e-19
Identity = 86/321 (26.79%), Postives = 127/321 (39.56%), Query Frame = 0

Query: 182 EKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNF 241
           E   L L  +G+L L    G V W+T T+ +GV   +IL  GN+ L D      WQSF+ 
Sbjct: 105 ENATLSLGRNGNLVLAEADGRVKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDH 164

Query: 242 PTDVMLLGQSLNAN--THLTSFPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSY 301
           PTD +L GQSL  N    L S  +++N +      YS  ++ + L +Y+N       Y  
Sbjct: 165 PTDTLLTGQSLKVNGVNKLVSRTSDSNGSDGP---YSMVLDKKGLTMYVNKTGTPLVYGG 224

Query: 302 WEFNPPNNINLSFITLNTQGL------DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNL 361
           W  +         +T     L      ++  + A + AT P  + + L+   +G+  G L
Sbjct: 225 WPDHDFRGTVTFAVTREFDNLTEPSAYELLLEPAPQPATNPGNNRRLLQVRPIGSGGGTL 284

Query: 362 GL----------------------YSYSPQKGI--FEASFRALKT----TCDLPLACKPY 421
            L                      YSY P      +E SF    T     C LP  C  Y
Sbjct: 285 NLNKINYNGTISYLRLGSDGSLKAYSYFPAATYLKWEESFSFFSTYFVRQCGLPSFCGDY 344

Query: 422 GICT--FSNSCSCIRVEMG-NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN 459
           G C     N+C   +  +G + KC   +     FC G++ + +    I  +       VN
Sbjct: 345 GYCDRGMCNACPTPKGLLGWSDKCAPPKTTQ--FCSGVKGKTVNYYKIVGVEHFTGPYVN 404

BLAST of MC05g_new0400 vs. ExPASy Swiss-Prot
Match: Q9LZR8 (PAN domain-containing protein At5g03700 OS=Arabidopsis thaliana OX=3702 GN=At5g03700 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 8.4e-17
Identity = 88/370 (23.78%), Postives = 158/370 (42.70%), Query Frame = 0

Query: 191 DGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQ 250
           +G L +  P+  + W T T+G   ++L +    NL +V     ++W+SF+FP + ++  Q
Sbjct: 109 NGSLVIIDPSSRLEWSTHTNG---DRLILRNDSNLQVVKTSTFVEWESFDFPGNTLVESQ 168

Query: 251 SLNANTHLTSFPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNP------ 310
           +           T+A A  +    YS  +    + LY    S +S+  YW+ +       
Sbjct: 169 NF----------TSAMALVSPNGLYSMRLGSDFIGLYAKV-SEESQQFYWKHSALQAKAK 228

Query: 311 -PNNINLSFITLNTQG-LDIFSDQAKKIATIPSQS-EQPLRFLALGNTTGNLGLYSYSPQ 370
             +        +N  G L ++   +  I      S ++P+  L +     +  L  Y   
Sbjct: 229 VKDGAGPILARINPNGYLGMYQTGSIPIDVEAFNSFQRPVNGLLILRLESDGNLRGYLWD 288

Query: 371 KGIFEASFRALKTTCDLPLACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCG-GI 430
              +  ++ A++ TCDLP  C PY +CT  + CSCI       +C     +   FC    
Sbjct: 289 GSHWALNYEAIRETCDLPNPCGPYSLCTPGSGCSCIDNRTVIGECTHAASSPADFCDKTT 348

Query: 431 EAEMIELEGISSILRE-APKRVNVSKEECGIWCLEDCKCAAALHYSGGGGGGGECYAYRV 490
           E +++  +G+    +E    +   S  EC   C+++CKC  A++ +G G      Y  R 
Sbjct: 349 EFKVVRRDGVEVPFKELMDHKTTSSLGECEEMCVDNCKCFGAVYNNGSGFCYLVNYPIRT 408

Query: 491 VMGVKQIEREREMGLSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYY 550
           ++GV    +     L Y  KV +  G+G+ KS +   +   +  V A +++     +G  
Sbjct: 409 MLGVADPSK-----LGYF-KVRE--GVGKKKSRVGLTVGMSLLAVIALVLMVAMVYVG-- 454

BLAST of MC05g_new0400 vs. ExPASy Swiss-Prot
Match: Q39688 (Epidermis-specific secreted glycoprotein EP1 OS=Daucus carota OX=4039 GN=EP1 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.9e-16
Identity = 71/252 (28.17%), Postives = 108/252 (42.86%), Query Frame = 0

Query: 182 EKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNF 241
           E   L    DG+L L    G V W+T T+ +GV  L+IL  GN+ L D+     WQSF+ 
Sbjct: 102 ENATLTFGPDGNLVLARSNGQVAWQTSTANKGVVGLKILPNGNMVLYDSKGKFLWQSFDT 161

Query: 242 PTDVMLLGQSLNANTHLTSFPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWE 301
           PTD +L+GQSL     +T   + A+   N    YS  +EP+ L LY    ++     Y+ 
Sbjct: 162 PTDTLLVGQSLKMGA-VTKLVSRASPGENVNGPYSLVMEPKGLHLYYKPTTSPKPIRYYS 221

Query: 302 FN---------PPNNINLSFITLNTQG------LDIFSDQAKKIATIPSQSE--QPLRFL 361
           F+            N+   F   N QG      L   +  +   A+I ++ +    L FL
Sbjct: 222 FSLFTKLNKNESLQNVTFEFENENDQGFAFLLSLKYGTSNSLGGASILNRIKYNTTLSFL 281

Query: 362 ALGNTTGNLGLYSYSPQ-----------------KGIFEASFRALK---TTCDLPLACKP 397
            L    GN+ +Y+Y+ +                   +F+ S  A +   + C LP  C  
Sbjct: 282 RL-EIDGNVKIYTYNDKVDYGAWEVTYTLFLKAPPPLFQVSLAATESESSECQLPKKCGN 341

BLAST of MC05g_new0400 vs. NCBI nr
Match: XP_022157315.1 (EP1-like glycoprotein 4, partial [Momordica charantia])

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 438/439 (99.77%), Postives = 438/439 (99.77%), Query Frame = 0

Query: 30  MGSLSLSLSLSLSLIVRAGKISPENSNAPPPANIQFLLVVDFEKFVIHFRIPPMKVAAQF 89
           MGSLSLSLSLSLSLIVRAGKISPENSNAPPPANIQFLLVVDFEKFVIHFRIPPMKVAAQF
Sbjct: 1   MGSLSLSLSLSLSLIVRAGKISPENSNAPPPANIQFLLVVDFEKFVIHFRIPPMKVAAQF 60

Query: 90  CFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRAALAV 149
           CFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRAALAV
Sbjct: 61  CFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRAALAV 120

Query: 150 EAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGT 209
           EAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGT
Sbjct: 121 EAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGT 180

Query: 210 SGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANA 269
           SGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANA
Sbjct: 181 SGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANA 240

Query: 270 NATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSDQAKK 329
           NATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSDQAKK
Sbjct: 241 NATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSDQAKK 300

Query: 330 IATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPYGICT 389
           IATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPYGICT
Sbjct: 301 IATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPYGICT 360

Query: 390 FSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVNVSKEECG 449
           FSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILR APKRVNVSKEECG
Sbjct: 361 FSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILRGAPKRVNVSKEECG 420

Query: 450 IWCLEDCKCAAALHYSGGG 468
           IWCLEDCKCAAALHYSGGG
Sbjct: 421 IWCLEDCKCAAALHYSGGG 439

BLAST of MC05g_new0400 vs. NCBI nr
Match: XP_022971607.1 (PAN domain-containing protein At5g03700 [Cucurbita maxima])

HSP 1 Score: 706 bits (1822), Expect = 3.73e-251
Identity = 364/481 (75.68%), Postives = 403/481 (83.78%), Query Frame = 0

Query: 83  MKVA--AQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 142
           MKVA  AQ CF++++L NL    CRTDI  G+ VTLAVPAEYGEGFIGRAFLIET+ + P
Sbjct: 1   MKVASAAQICFVLLLL-NLDRTICRTDIVPGHDVTLAVPAEYGEGFIGRAFLIETEHLPP 60

Query: 143 PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 202
           P+FRAAL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP 
Sbjct: 61  PNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPT 120

Query: 203 GHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTS 262
           GHVGWRTGT+GQGVEKLRILR+GNLALVDAL+ +KWQSFNFPTDV+LLGQSLN  THLTS
Sbjct: 121 GHVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTS 180

Query: 263 FPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGL 322
           FP N+      TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GL
Sbjct: 181 FPPNS------TSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGL 240

Query: 323 DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPL 382
           DIF+DQA KIA IPS + QPLRF+ALGN +GNLGLY YSPQKGIFEAS RALKTTCDLPL
Sbjct: 241 DIFNDQAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSPQKGIFEASNRALKTTCDLPL 300

Query: 383 ACKPYGICTFSNSCSCIRVEMGN----SKCGGEEINSGGFCGGIEAEMIELEGISSILRE 442
           ACKPYGICTFSNSCSCI  ++ N    SKC  +EI SG FCGGIE EM+ELEGISSILR+
Sbjct: 301 ACKPYGICTFSNSCSCITFQVENEGDSSKCS-DEI-SGKFCGGIEGEMVELEGISSILRD 360

Query: 443 APKRVNVSKEECGIWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREM 502
           AP RVN+SK ECG WCLEDCKCAAALHYSGGG    GGG ECY YR+VMGVK+IE+   M
Sbjct: 361 APNRVNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRLVMGVKEIEKG--M 420

Query: 503 GLSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTD 553
           G SYMVKVPKG+ L R KS LKKW+L VVGVVD  +I+AV GGLGYYFI RRR NL+  D
Sbjct: 421 GFSYMVKVPKGTALERRKSGLKKWVLAVVGVVDGLVIVAVCGGLGYYFIKRRRKNLILRD 468

BLAST of MC05g_new0400 vs. NCBI nr
Match: KAG6596316.1 (putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027869.1 putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 704 bits (1818), Expect = 1.51e-250
Identity = 360/479 (75.16%), Postives = 402/479 (83.92%), Query Frame = 0

Query: 86  AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRA 145
           AAQ CF++++L NL  A CRTDI  G++VTLAVPAEYGEGFIGRAFLIET+ + PP+FRA
Sbjct: 6   AAQICFVLLLL-NLDRAICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPPPNFRA 65

Query: 146 ALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGW 205
           AL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP GHVGW
Sbjct: 66  ALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPTGHVGW 125

Query: 206 RTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNA 265
           RTGT+GQGVEKLRILR+GNLALVDAL+ +KWQSFNFPTDV+LLGQSLN  THLTSFP N+
Sbjct: 126 RTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTSFPPNS 185

Query: 266 NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSD 325
                 TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDIF+D
Sbjct: 186 ------TSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDIFND 245

Query: 326 QAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPY 385
           +A KIA IPS + QPLRF+ALGN +GNLGLY YS QKGIFEAS RALKTTCDLPLACKPY
Sbjct: 246 EAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSTQKGIFEASNRALKTTCDLPLACKPY 305

Query: 386 GICTFSNSCSCIRVEM-----GNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 445
           GICTFSNSCSCI  ++      NSKC  +EI SG FCGGIE EM+ELEGISSILR+AP R
Sbjct: 306 GICTFSNSCSCITFQVENEGDDNSKCS-DEI-SGKFCGGIEGEMVELEGISSILRDAPNR 365

Query: 446 VNVSKEECGIWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREMGLSY 505
           VN+SK ECG WCLEDCKCAAALHYSGGG    GGG ECY YRVVMGVK+IE+   MG SY
Sbjct: 366 VNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRVVMGVKEIEKG--MGFSY 425

Query: 506 MVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDTHS 555
           MVKVPKG+ L R KS LKKW+L  VGVVD  +I+AV GGLGYYFI RRR NL+  DT++
Sbjct: 426 MVKVPKGTALERRKSGLKKWVLAAVGVVDGLVIVAVCGGLGYYFIKRRRKNLILRDTNN 471

BLAST of MC05g_new0400 vs. NCBI nr
Match: XP_022933279.1 (EP1-like glycoprotein 4 [Cucurbita moschata])

HSP 1 Score: 704 bits (1817), Expect = 2.15e-250
Identity = 360/479 (75.16%), Postives = 402/479 (83.92%), Query Frame = 0

Query: 86  AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRA 145
           AAQ CF++++L NL  A CRTDI  G++VTLAVPAEYGEGFIGRAFLIET+ + PP+FRA
Sbjct: 6   AAQICFVLLLL-NLDRAICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPPPNFRA 65

Query: 146 ALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGW 205
           AL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP GHVGW
Sbjct: 66  ALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPTGHVGW 125

Query: 206 RTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNA 265
           RTGT+GQGVEKLRILR+GNLALVDAL+ +KWQSFNFPTDV+LLGQSLN  THLTSFP N+
Sbjct: 126 RTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTSFPPNS 185

Query: 266 NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSD 325
                 TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDIF+D
Sbjct: 186 ------TSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDIFND 245

Query: 326 QAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPY 385
           +A KIA IPS + QPLRF+ALGN +GNLGLY YS QKGIFEAS RALKTTCDLPLACKPY
Sbjct: 246 EAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSTQKGIFEASNRALKTTCDLPLACKPY 305

Query: 386 GICTFSNSCSCIRVEM-----GNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 445
           GICTFSNSCSCI  ++      NSKC  +EI SG FCGGIE EM+ELEGISSILR+AP R
Sbjct: 306 GICTFSNSCSCITFQVENEGDDNSKCS-DEI-SGKFCGGIEGEMVELEGISSILRDAPNR 365

Query: 446 VNVSKEECGIWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREMGLSY 505
           VN+SK ECG WCLEDCKCAAALHYSGGG    GGG ECY YRVVMGVK+IE+   MG SY
Sbjct: 366 VNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRVVMGVKEIEKG--MGFSY 425

Query: 506 MVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDTHS 555
           MVKVPKG+ L R KS LKKW+L  VGVVD  +I+AV GGLGYYFI RRR NL+  DT++
Sbjct: 426 MVKVPKGTALERRKSGLKKWVLAAVGVVDGLVIVAVCGGLGYYFIKRRRKNLILGDTNN 471

BLAST of MC05g_new0400 vs. NCBI nr
Match: XP_023540139.1 (EP1-like glycoprotein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 692 bits (1786), Expect = 1.15e-245
Identity = 353/480 (73.54%), Postives = 398/480 (82.92%), Query Frame = 0

Query: 86  AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRA 145
           AAQ CF++++L NL    CRTDI  G++VTLAVPAEYGEGFIGRAFLIET+ + PP+FRA
Sbjct: 6   AAQICFVLLLL-NLDRTICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPPPNFRA 65

Query: 146 ALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGW 205
           AL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP GHVGW
Sbjct: 66  ALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPTGHVGW 125

Query: 206 RTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNA 265
           RTGT+GQGVEKLRILR+GNLALVDA + +KWQSFNFPTDV+LLGQSLN  THLTSFP N+
Sbjct: 126 RTGTAGQGVEKLRILRSGNLALVDASDGVKWQSFNFPTDVLLLGQSLNVATHLTSFPPNS 185

Query: 266 NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSD 325
                 TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDIF+D
Sbjct: 186 ------TSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDIFND 245

Query: 326 QAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPY 385
           +A KIA IPS +  PLRF+ALGN +GNLGLY YS QKGIFEAS RALKTTCDLPLACKPY
Sbjct: 246 EAMKIAAIPSGTAHPLRFVALGNKSGNLGLYYYSTQKGIFEASNRALKTTCDLPLACKPY 305

Query: 386 GICTFSNSCSCIRVEM-----GNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 445
           GICTFSNSCSCI  ++      NSKC  ++I SG FCGG E EM+ELEGISSILR+AP R
Sbjct: 306 GICTFSNSCSCITFQVENEGDDNSKCS-DQI-SGKFCGGTEGEMVELEGISSILRDAPNR 365

Query: 446 VNVSKEECGIWCLEDCKCAAALHYSGG-----GGGGGECYAYRVVMGVKQIEREREMGLS 505
           VN+SK ECG WCLEDCKCAAALHYSGG     GGGG ECY YRVVMGVK+IE+   MG +
Sbjct: 366 VNLSKRECGNWCLEDCKCAAALHYSGGDDGGGGGGGEECYLYRVVMGVKEIEKG--MGFN 425

Query: 506 YMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDTHS 555
           YMVKVPKG+ L R KS LKKW+L  VGVVD  +I+AV GGLGYYFI RRR +L+  DT++
Sbjct: 426 YMVKVPKGTALERRKSGLKKWVLAAVGVVDGLVIVAVCGGLGYYFIKRRRKSLILRDTNN 472

BLAST of MC05g_new0400 vs. ExPASy TrEMBL
Match: A0A6J1DSQ6 (EP1-like glycoprotein 4 OS=Momordica charantia OX=3673 GN=LOC111024046 PE=4 SV=1)

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 438/439 (99.77%), Postives = 438/439 (99.77%), Query Frame = 0

Query: 30  MGSLSLSLSLSLSLIVRAGKISPENSNAPPPANIQFLLVVDFEKFVIHFRIPPMKVAAQF 89
           MGSLSLSLSLSLSLIVRAGKISPENSNAPPPANIQFLLVVDFEKFVIHFRIPPMKVAAQF
Sbjct: 1   MGSLSLSLSLSLSLIVRAGKISPENSNAPPPANIQFLLVVDFEKFVIHFRIPPMKVAAQF 60

Query: 90  CFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRAALAV 149
           CFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRAALAV
Sbjct: 61  CFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRAALAV 120

Query: 150 EAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGT 209
           EAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGT
Sbjct: 121 EAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGT 180

Query: 210 SGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANA 269
           SGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANA
Sbjct: 181 SGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANA 240

Query: 270 NATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSDQAKK 329
           NATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSDQAKK
Sbjct: 241 NATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSDQAKK 300

Query: 330 IATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPYGICT 389
           IATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPYGICT
Sbjct: 301 IATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPYGICT 360

Query: 390 FSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVNVSKEECG 449
           FSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILR APKRVNVSKEECG
Sbjct: 361 FSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILRGAPKRVNVSKEECG 420

Query: 450 IWCLEDCKCAAALHYSGGG 468
           IWCLEDCKCAAALHYSGGG
Sbjct: 421 IWCLEDCKCAAALHYSGGG 439

BLAST of MC05g_new0400 vs. ExPASy TrEMBL
Match: A0A6J1I2F5 (PAN domain-containing protein At5g03700 OS=Cucurbita maxima OX=3661 GN=LOC111470282 PE=4 SV=1)

HSP 1 Score: 706 bits (1822), Expect = 1.81e-251
Identity = 364/481 (75.68%), Postives = 403/481 (83.78%), Query Frame = 0

Query: 83  MKVA--AQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITP 142
           MKVA  AQ CF++++L NL    CRTDI  G+ VTLAVPAEYGEGFIGRAFLIET+ + P
Sbjct: 1   MKVASAAQICFVLLLL-NLDRTICRTDIVPGHDVTLAVPAEYGEGFIGRAFLIETEHLPP 60

Query: 143 PSFRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPA 202
           P+FRAAL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP 
Sbjct: 61  PNFRAALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPT 120

Query: 203 GHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTS 262
           GHVGWRTGT+GQGVEKLRILR+GNLALVDAL+ +KWQSFNFPTDV+LLGQSLN  THLTS
Sbjct: 121 GHVGWRTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTS 180

Query: 263 FPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGL 322
           FP N+      TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GL
Sbjct: 181 FPPNS------TSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGL 240

Query: 323 DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPL 382
           DIF+DQA KIA IPS + QPLRF+ALGN +GNLGLY YSPQKGIFEAS RALKTTCDLPL
Sbjct: 241 DIFNDQAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSPQKGIFEASNRALKTTCDLPL 300

Query: 383 ACKPYGICTFSNSCSCIRVEMGN----SKCGGEEINSGGFCGGIEAEMIELEGISSILRE 442
           ACKPYGICTFSNSCSCI  ++ N    SKC  +EI SG FCGGIE EM+ELEGISSILR+
Sbjct: 301 ACKPYGICTFSNSCSCITFQVENEGDSSKCS-DEI-SGKFCGGIEGEMVELEGISSILRD 360

Query: 443 APKRVNVSKEECGIWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREM 502
           AP RVN+SK ECG WCLEDCKCAAALHYSGGG    GGG ECY YR+VMGVK+IE+   M
Sbjct: 361 APNRVNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRLVMGVKEIEKG--M 420

Query: 503 GLSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTD 553
           G SYMVKVPKG+ L R KS LKKW+L VVGVVD  +I+AV GGLGYYFI RRR NL+  D
Sbjct: 421 GFSYMVKVPKGTALERRKSGLKKWVLAVVGVVDGLVIVAVCGGLGYYFIKRRRKNLILRD 468

BLAST of MC05g_new0400 vs. ExPASy TrEMBL
Match: A0A6J1EZB2 (EP1-like glycoprotein 4 OS=Cucurbita moschata OX=3662 GN=LOC111440327 PE=4 SV=1)

HSP 1 Score: 704 bits (1817), Expect = 1.04e-250
Identity = 360/479 (75.16%), Postives = 402/479 (83.92%), Query Frame = 0

Query: 86  AAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPSFRA 145
           AAQ CF++++L NL  A CRTDI  G++VTLAVPAEYGEGFIGRAFLIET+ + PP+FRA
Sbjct: 6   AAQICFVLLLL-NLDRAICRTDIVPGHEVTLAVPAEYGEGFIGRAFLIETEHLPPPNFRA 65

Query: 146 ALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGW 205
           AL VEA +GNFSCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP GHVGW
Sbjct: 66  ALTVEATQGNFSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTDDGDLRLKGPTGHVGW 125

Query: 206 RTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNA 265
           RTGT+GQGVEKLRILR+GNLALVDAL+ +KWQSFNFPTDV+LLGQSLN  THLTSFP N+
Sbjct: 126 RTGTAGQGVEKLRILRSGNLALVDALDGVKWQSFNFPTDVLLLGQSLNVATHLTSFPPNS 185

Query: 266 NANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDIFSD 325
                 TSFYSFEI+ Q+LAL++  NSAKSKYSYWEF PP N+NLSFITLNT GLDIF+D
Sbjct: 186 ------TSFYSFEIQAQKLALFL--NSAKSKYSYWEFKPPKNMNLSFITLNTDGLDIFND 245

Query: 326 QAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLACKPY 385
           +A KIA IPS + QPLRF+ALGN +GNLGLY YS QKGIFEAS RALKTTCDLPLACKPY
Sbjct: 246 EAMKIAAIPSGTAQPLRFVALGNKSGNLGLYYYSTQKGIFEASNRALKTTCDLPLACKPY 305

Query: 386 GICTFSNSCSCIRVEM-----GNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 445
           GICTFSNSCSCI  ++      NSKC  +EI SG FCGGIE EM+ELEGISSILR+AP R
Sbjct: 306 GICTFSNSCSCITFQVENEGDDNSKCS-DEI-SGKFCGGIEGEMVELEGISSILRDAPNR 365

Query: 446 VNVSKEECGIWCLEDCKCAAALHYSGGG----GGGGECYAYRVVMGVKQIEREREMGLSY 505
           VN+SK ECG WCLEDCKCAAALHYSGGG    GGG ECY YRVVMGVK+IE+   MG SY
Sbjct: 366 VNLSKRECGNWCLEDCKCAAALHYSGGGDGGVGGGEECYLYRVVMGVKEIEKG--MGFSY 425

Query: 506 MVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLVDTDTHS 555
           MVKVPKG+ L R KS LKKW+L  VGVVD  +I+AV GGLGYYFI RRR NL+  DT++
Sbjct: 426 MVKVPKGTALERRKSGLKKWVLAAVGVVDGLVIVAVCGGLGYYFIKRRRKNLILGDTNN 471

BLAST of MC05g_new0400 vs. ExPASy TrEMBL
Match: A0A5D3DNN1 (PAN domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00330 PE=4 SV=1)

HSP 1 Score: 663 bits (1711), Expect = 6.47e-235
Identity = 342/474 (72.15%), Postives = 379/474 (79.96%), Query Frame = 0

Query: 83  MKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPS 142
           MKVA  FC L +  FNL  +  RTD+ TGY+V LAVPAEY EGFIGRAFL+E++ + PP+
Sbjct: 1   MKVAPGFCLLFIFCFNLSPSISRTDLVTGYEVHLAVPAEYVEGFIGRAFLMESENLMPPN 60

Query: 143 FRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGH 202
           FRAALA+EA +G +SCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP GH
Sbjct: 61  FRAALAIEATQGQYSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTADGDLRLKGPTGH 120

Query: 203 VGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFP 262
           VGWRTGTS QGVE+LRILR GNLALVDA+E IKWQSFNFPTDVM+LGQSLN  THLTSFP
Sbjct: 121 VGWRTGTSRQGVERLRILRNGNLALVDAIEGIKWQSFNFPTDVMVLGQSLNVKTHLTSFP 180

Query: 263 TNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDI 322
            ++        FYSFEI+ QR+ALY+  NS K KYSYWEF PPNNINLS+ITLN +GLD 
Sbjct: 181 PHS------IFFYSFEIQTQRIALYL--NSPKCKYSYWEFKPPNNINLSYITLNPEGLDF 240

Query: 323 FSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLAC 382
           F D+A KIATIPS +  PLRFLALGN TGNLGLYSYSPQ GIFEASFRAL TTCDLPLAC
Sbjct: 241 FDDRANKIATIPSGTPHPLRFLALGNKTGNLGLYSYSPQNGIFEASFRALTTTCDLPLAC 300

Query: 383 KPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN 442
           KPYGICTFSNSCSCI      SKC   E   G FC   + EM+EL G+SSILR+ PKRVN
Sbjct: 301 KPYGICTFSNSCSCI-----GSKC--REEMGGEFCE-AKGEMMELVGVSSILRDGPKRVN 360

Query: 443 VSKEECGIWCLEDCKCAAALHYSGGGGGGGECYAYRVVMGVKQIEREREMGLSYMVKVPK 502
           VSKEECG WCL+DCKC AALHYS       ECY YRVV+GVKQIE+   MGLSYMVKVPK
Sbjct: 361 VSKEECGEWCLDDCKCVAALHYSVME----ECYLYRVVIGVKQIEKG--MGLSYMVKVPK 420

Query: 503 GSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRN-NLVDTDTHS 555
           G+ LG HKS LK+W+L VVGVVD  +ILAV GGL YYF+ RRR  NL DTD HS
Sbjct: 421 GTALGSHKSGLKRWVLAVVGVVDGVVILAVSGGLAYYFVKRRRKKNLTDTDVHS 452

BLAST of MC05g_new0400 vs. ExPASy TrEMBL
Match: A0A5A7TQ35 (PAN domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005760 PE=4 SV=1)

HSP 1 Score: 663 bits (1710), Expect = 9.19e-235
Identity = 343/474 (72.36%), Postives = 378/474 (79.75%), Query Frame = 0

Query: 83  MKVAAQFCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQITPPS 142
           MKVA  FC L +  FNL  +  RTD+ TGY+V LAVPAEY EGFIGRAFL+E++ + PP+
Sbjct: 1   MKVAPGFCLLFIFCFNLSPSISRTDLVTGYEVHLAVPAEYVEGFIGRAFLMESENLMPPN 60

Query: 143 FRAALAVEAIEGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGH 202
           FRAALA+EA +G +SCSLQVFLG VKVWSSGHFSRF+TAEKCVLELT DGDLRLKGP GH
Sbjct: 61  FRAALAIEATQGQYSCSLQVFLGEVKVWSSGHFSRFFTAEKCVLELTADGDLRLKGPTGH 120

Query: 203 VGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFP 262
           VGWRTGTS QGVE+LRILR GNLALVDA+E IKWQSFNFPTDVM+LGQSLN  THLTSFP
Sbjct: 121 VGWRTGTSRQGVERLRILRNGNLALVDAIEGIKWQSFNFPTDVMVLGQSLNVKTHLTSFP 180

Query: 263 TNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNPPNNINLSFITLNTQGLDI 322
            ++        FYSFEI+ QR+ALY   NS K KYSYWEF PPNNINLS+ITLN +GLD 
Sbjct: 181 PHS------IFFYSFEIQTQRIALYF--NSPKCKYSYWEFKPPNNINLSYITLNPEGLDF 240

Query: 323 FSDQAKKIATIPSQSEQPLRFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLAC 382
           F D+A KIATIPS +  PLRFLALGN TGNLGLYSYSPQ GIFEASFRAL TTCDLPLAC
Sbjct: 241 FDDRANKIATIPSGTPHPLRFLALGNKTGNLGLYSYSPQNGIFEASFRALTTTCDLPLAC 300

Query: 383 KPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN 442
           KPYGICTFSNSCSCI      SKC   E   G FC   + EM+EL G+SSILR+ PKRVN
Sbjct: 301 KPYGICTFSNSCSCI-----GSKC--REEMGGEFCE-AKGEMMELVGVSSILRDGPKRVN 360

Query: 443 VSKEECGIWCLEDCKCAAALHYSGGGGGGGECYAYRVVMGVKQIEREREMGLSYMVKVPK 502
           VSKEECG WCL+DCKC AALHYS       ECY YRVV+GVKQIE+   MGLSYMVKVPK
Sbjct: 361 VSKEECGEWCLDDCKCVAALHYSVME----ECYLYRVVIGVKQIEKG--MGLSYMVKVPK 420

Query: 503 GSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRN-NLVDTDTHS 555
           G+ LG HKS LK+W+L VVGVVD  +ILAV GGL YYFI RRR  NL DTD HS
Sbjct: 421 GTALGSHKSGLKRWVLAVVGVVDGVVILAVSGGLAYYFIKRRRRKNLTDTDVHS 452

BLAST of MC05g_new0400 vs. TAIR 10
Match: AT3G51710.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 459.1 bits (1180), Expect = 4.9e-129
Identity = 244/472 (51.69%), Postives = 319/472 (67.58%), Query Frame = 0

Query: 89  FCFLIVVLFNLGHAHCRTDIFTGYQVTLAVPAEYGEGFIGRAFLIETQQIT--PPSFRAA 148
           F FLI  LF+    HC++DI  G  +TL  P EY  GF+G+A++IET+  +   P F+AA
Sbjct: 8   FTFLI-CLFSKLQGHCKSDISLGNSLTLTSPLEYTPGFMGKAYIIETESSSTREPGFKAA 67

Query: 149 LAVEAI---EGNFSCSLQVFLGGVKVWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHV 208
           L +E+    +G + CSLQ+FLG V+VWSSGH+S+ Y + KC++ELT DGDLRLK    HV
Sbjct: 68  LTMESSDKDDGRYLCSLQIFLGDVRVWSSGHYSKMYVSSKCIIELTKDGDLRLKSSYKHV 127

Query: 209 GWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPT 268
           GWR+GTSGQGVE+L I  TGNL LVDA   IKWQSFNFPTDVML GQ L+  T LTSFP 
Sbjct: 128 GWRSGTSGQGVERLEIQSTGNLVLVDAKNLIKWQSFNFPTDVMLSGQRLDVATQLTSFPN 187

Query: 269 NANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNP-PNNINLSFITLNTQGLDI 328
                 ++T FYSFE+   ++AL++N N  K KYSYWE+ P   N  ++F+ L  +GLD+
Sbjct: 188 ------DSTLFYSFEVLRDKIALFLNLN--KLKYSYWEYKPREKNTTVNFVRLGLKGLDL 247

Query: 329 FSDQAKKIATIPSQSEQPL-RFLALGNTTGNLGLYSYSPQKGIFEASFRALKTTCDLPLA 388
           F D ++ I  I    EQPL RFLALGN TGNLGLYSY P+KG FEA+F+A+  TCDLP+A
Sbjct: 248 FDDNSRIIGRI----EQPLIRFLALGNRTGNLGLYSYKPEKGKFEATFQAVSDTCDLPVA 307

Query: 389 CKPYGICTFSNSCSCIRVEMGNSKC----GGEEINSGGFCGGIEAEMIELEGISSILREA 448
           CKPYGICTFS SCSCI+V + N  C    G E ++    C   + EM+EL G++++LR  
Sbjct: 308 CKPYGICTFSKSCSCIKV-VSNGYCSSINGEEAVSVKRLC---DHEMVELNGVTTVLRNG 367

Query: 449 PKRVNVSKEECGIWCLEDCKCAAALHYSGGGGGGGECYAYRVVMGVKQIEREREMGLSYM 508
            +  N+SKE C   C +DC+C AA +          C  Y +VMGVKQI  ER  GLSYM
Sbjct: 368 TQVRNISKERCEELCKKDCECGAASY----SVSEESCVMYGIVMGVKQI--ERVSGLSYM 427

Query: 509 VKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYYFINRRRNNLV 550
           VK+PKG  L   KS+++KW++ +VG +D F+IL +  G  +YFI +RR +L+
Sbjct: 428 VKIPKGVRLSDEKSNVRKWVVGLVGGIDGFVILLLISGFAFYFIRKRRKSLL 456

BLAST of MC05g_new0400 vs. TAIR 10
Match: AT1G78860.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 100.9 bits (250), Expect = 3.4e-21
Identity = 85/318 (26.73%), Postives = 137/318 (43.08%), Query Frame = 0

Query: 169 VWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALV 228
           VW +   S     E   L    DG+L L    G V W+T T+ +GV  ++IL  GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRVVWQTNTANKGVVGIKILENGNMVIY 149

Query: 229 DALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANANATSFYSFEIEPQRLALYI 288
           D+     WQSF+ PTD +L+GQSL  N       +  + + NA   YS  +E ++L LY 
Sbjct: 150 DSNGKFVWQSFDSPTDTLLVGQSLKLNGQ-NKLVSRLSPSVNANGPYSLVMEAKKLVLYY 209

Query: 289 NSNSAKSKYSYWEFNPPNNI-NLSFIT------------LNTQGLDIFSDQAKKIATIPS 348
            +N       Y+E+     I  L  +T            L+ +G+D  S     ++T  S
Sbjct: 210 TTNKTPKPIGYYEYEFFTKIAQLQSMTFQAVEDADTTWGLHMEGVD--SGSQFNVSTFLS 269

Query: 349 QSEQ--PLRFLALGNTTGNLGLYSYS--PQKGIFEASFRAL-------KTTCDLPLACKP 408
           + +    L FL L  + GN+ ++SYS       ++ ++ A           C +P  C  
Sbjct: 270 RPKHNATLSFLRL-ESDGNIRVWSYSTLATSTAWDVTYTAFTNDNTDGNDECRIPEHCLG 329

Query: 409 YGICTFSNSCSCIRVEMG----NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKR 459
           +G+C     C+    ++G    +  C   +I S   C        ++EG  S + +    
Sbjct: 330 FGLCK-KGQCNACPSDIGLLGWDETC---KIPSLASCDPKTFHYFKIEGADSFMTKYNGG 389

BLAST of MC05g_new0400 vs. TAIR 10
Match: AT1G78850.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 98.2 bits (243), Expect = 2.2e-20
Identity = 79/316 (25.00%), Postives = 136/316 (43.04%), Query Frame = 0

Query: 169 VWSSGHFSRFYTAEKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALV 228
           VW +   S     E   L    DG+L L    G + W+T T+ +G   ++IL  GN+ + 
Sbjct: 90  VWEANRGSP--VKENATLTFGEDGNLVLAEADGRLVWQTNTANKGAVGIKILENGNMVIY 149

Query: 229 DALESIKWQSFNFPTDVMLLGQSLNANTHLTSFPTNANANANATSFYSFEIEPQRLALYI 288
           D+     WQSF+ PTD +L+GQSL  N   T   +  + + N    YS  +E ++L LY 
Sbjct: 150 DSSGKFVWQSFDSPTDTLLVGQSLKLNGR-TKLVSRLSPSVNTNGPYSLVMEAKKLVLYY 209

Query: 289 NSNSAKSKYSYWEFNPPNNINLSFITLNTQGLD------------IFSDQAKKIATIPSQ 348
            +N      +Y+E+     I   F ++  Q ++            + S     ++T  S+
Sbjct: 210 TTNKTPKPIAYFEYEFFTKIT-QFQSMTFQAVEDSDTTWGLVMEGVDSGSKFNVSTFLSR 269

Query: 349 SEQ--PLRFLALGNTTGNLGLYSYS--PQKGIFEASFRALKTT-------CDLPLACKPY 408
            +    L F+ L  + GN+ ++SYS       ++ ++ A           C +P  C  +
Sbjct: 270 PKHNATLSFIRL-ESDGNIRVWSYSTLATSTAWDVTYTAFTNADTDGNDECRIPEHCLGF 329

Query: 409 GICTFS--NSCSCIRVEMG-NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN 459
           G+C     N+C   +  +G +  C    + S   C        ++EG  S + +     +
Sbjct: 330 GLCKKGQCNACPSDKGLLGWDETCKSPSLAS---CDPKTFHYFKIEGADSFMTKYNGGSS 389

BLAST of MC05g_new0400 vs. TAIR 10
Match: AT1G78830.1 (Curculin-like (mannose-binding) lectin family protein )

HSP 1 Score: 97.8 bits (242), Expect = 2.9e-20
Identity = 86/321 (26.79%), Postives = 127/321 (39.56%), Query Frame = 0

Query: 182 EKCVLELTGDGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNF 241
           E   L L  +G+L L    G V W+T T+ +GV   +IL  GN+ L D      WQSF+ 
Sbjct: 105 ENATLSLGRNGNLVLAEADGRVKWQTNTANKGVTGFQILPNGNIVLHDKNGKFVWQSFDH 164

Query: 242 PTDVMLLGQSLNAN--THLTSFPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSY 301
           PTD +L GQSL  N    L S  +++N +      YS  ++ + L +Y+N       Y  
Sbjct: 165 PTDTLLTGQSLKVNGVNKLVSRTSDSNGSDGP---YSMVLDKKGLTMYVNKTGTPLVYGG 224

Query: 302 WEFNPPNNINLSFITLNTQGL------DIFSDQAKKIATIPSQSEQPLRFLALGNTTGNL 361
           W  +         +T     L      ++  + A + AT P  + + L+   +G+  G L
Sbjct: 225 WPDHDFRGTVTFAVTREFDNLTEPSAYELLLEPAPQPATNPGNNRRLLQVRPIGSGGGTL 284

Query: 362 GL----------------------YSYSPQKGI--FEASFRALKT----TCDLPLACKPY 421
            L                      YSY P      +E SF    T     C LP  C  Y
Sbjct: 285 NLNKINYNGTISYLRLGSDGSLKAYSYFPAATYLKWEESFSFFSTYFVRQCGLPSFCGDY 344

Query: 422 GICT--FSNSCSCIRVEMG-NSKCGGEEINSGGFCGGIEAEMIELEGISSILREAPKRVN 459
           G C     N+C   +  +G + KC   +     FC G++ + +    I  +       VN
Sbjct: 345 GYCDRGMCNACPTPKGLLGWSDKCAPPKTTQ--FCSGVKGKTVNYYKIVGVEHFTGPYVN 404

BLAST of MC05g_new0400 vs. TAIR 10
Match: AT5G03700.1 (D-mannose binding lectin protein with Apple-like carbohydrate-binding domain )

HSP 1 Score: 90.1 bits (222), Expect = 6.0e-18
Identity = 88/370 (23.78%), Postives = 158/370 (42.70%), Query Frame = 0

Query: 191 DGDLRLKGPAGHVGWRTGTSGQGVEKLRILRTGNLALVDALESIKWQSFNFPTDVMLLGQ 250
           +G L +  P+  + W T T+G   ++L +    NL +V     ++W+SF+FP + ++  Q
Sbjct: 109 NGSLVIIDPSSRLEWSTHTNG---DRLILRNDSNLQVVKTSTFVEWESFDFPGNTLVESQ 168

Query: 251 SLNANTHLTSFPTNANANANATSFYSFEIEPQRLALYINSNSAKSKYSYWEFNP------ 310
           +           T+A A  +    YS  +    + LY    S +S+  YW+ +       
Sbjct: 169 NF----------TSAMALVSPNGLYSMRLGSDFIGLYAKV-SEESQQFYWKHSALQAKAK 228

Query: 311 -PNNINLSFITLNTQG-LDIFSDQAKKIATIPSQS-EQPLRFLALGNTTGNLGLYSYSPQ 370
             +        +N  G L ++   +  I      S ++P+  L +     +  L  Y   
Sbjct: 229 VKDGAGPILARINPNGYLGMYQTGSIPIDVEAFNSFQRPVNGLLILRLESDGNLRGYLWD 288

Query: 371 KGIFEASFRALKTTCDLPLACKPYGICTFSNSCSCIRVEMGNSKCGGEEINSGGFCG-GI 430
              +  ++ A++ TCDLP  C PY +CT  + CSCI       +C     +   FC    
Sbjct: 289 GSHWALNYEAIRETCDLPNPCGPYSLCTPGSGCSCIDNRTVIGECTHAASSPADFCDKTT 348

Query: 431 EAEMIELEGISSILRE-APKRVNVSKEECGIWCLEDCKCAAALHYSGGGGGGGECYAYRV 490
           E +++  +G+    +E    +   S  EC   C+++CKC  A++ +G G      Y  R 
Sbjct: 349 EFKVVRRDGVEVPFKELMDHKTTSSLGECEEMCVDNCKCFGAVYNNGSGFCYLVNYPIRT 408

Query: 491 VMGVKQIEREREMGLSYMVKVPKGSGLGRHKSSLKKWILPVVGVVDAFIILAVFGGLGYY 550
           ++GV    +     L Y  KV +  G+G+ KS +   +   +  V A +++     +G  
Sbjct: 409 MLGVADPSK-----LGYF-KVRE--GVGKKKSRVGLTVGMSLLAVIALVLMVAMVYVG-- 454