MC05g1040 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g1040
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein NRT1/ PTR FAMILY 3.1-like
LocationMC05: 14090993 .. 14095788 (-)
RNA-Seq ExpressionMC05g1040
SyntenyMC05g1040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTCAATTTTTGGAGGGCCCCACTTTCTGATTATGAGGATTATAAAACGGCACCGTATTGTGTGTCATTGGCCATATAAAGAGGGGTTATTGTGGAGGGAAGAAGTGGAGAGACAATGGAGATAGAATCCATTAGAGTGGGAGTTGATGAGAAAGAGAAGAAGAAATTAGAAGAAGAGATGGAATTAGAGGAAGGGGAGAAGAAGAAGGACAGGAAGCTTGGTGGAGTCAAGACAATGCCTTTCATTCTTGGTAACATCTCTCTCTCTCCCTTTGCATTTGGCTTCTCTTTCTCTCTCTAAAGACATGGGCAATACAGTCATTTCGGTTGCATTATGATTCTTTCGAGAGATATTTCGTATTCCGTTTTTGTTTTTGGTAAGTTGTAATATATATTGTGCATGAAGTTATTACGAGTGTAGGTGCGAGTGTTTGAATCTTCGACCGTAGAAAAGATTAGATTTTCAATACTCGACTATAACTTTATTTATTCTAAACATGTGAAGTCACTCACATGAAACACAATAGTATATATCCTTTTTAATTTCTAGAAAATAAAATATTATTTTGAATAGAATCTTTAGATGGAAGTTTTTGGATTGAGTGGTGGAATTAAATTTACTTAACTCATCAGCTTAAGCTTTTGAGTTGAGTGATGATTTATTCATCCTTTACAGGTAGATCGACCATGTTGAAAATAATAGATGAGATTTGTTCTCTTTTAACTTTTTAATATTTTATAAAGGATGAAAGTTAGATGATTACTATAGACACACACCATTAAAAAATTGACAATTAAAAGAAATAACAAAAGAGAATTAGAACAAATAACCATCCACTTTTTTTAATATAAATATGAAGAGAAAATTTACAAATAAGCATCTAAATTTTATCTTTTAAATATTAATCCTTCTATTTTTGTTCTCGTCTCTCTCTCTTAAACTAAAATCATTCAAGATAAAAGCATAAAAAAATGGAAAGTTTTTATTTTTCTCCTTTGGGCATTATATTATAAATATGAAATATTGTGCATGAGAGTCAAAGTTATTAATTAATAAATCAGCTAAGAAATATTATATTCTTTTTTTGCAAAGGGAAATATTATATTCTATGATATAATATTTTATCTCAAAAAATAAGTAAACAATATAATATATATATTTGGACAGCTACATGAATCTTGAACCTTACTTTTTTGTCCCATTCTTGGGAACACAATACTTTCCCCTCGTGATTACTGAGTAAGGTAATAATTTATTTTAAAAAATGTCAAAGGCAAAAATATTTCAACTAATTAATATAATAAATTTTATCTCAAACAAGAAATAATAATAATAATAATATAATAACTTTTTTTTTAATTTTTATTTTTACAATTAATACAATTAAAAACTAAAACACACACTCACGTGCATAACACTCAACAAATAGGAACTCATGACTTCTTGATCAGATACAAATATCAATTACCTCTGGTTAAGTTCATATTTGATTTTATCAAAATTCTAATGAAAAATTTTAATTTAAAAGTTCAAAACTAAAATAAAACAGTCACTAATCTTGACTTTTAGTTTGGTCTTATATGATAGTATAAATTTTTAATTTATGTCATTTTTGTAAAATTTTGAATCTAATGCTATCTATTTTTGTAGTTTCCTTTGTATATATTGTGAAATATATTATGACATATTTTAGAGTAATTTTGACATGTTTTAACTCATACTTTACTTTTCCACCATCAAAATTAGTTTCATTTAAAATAGTACCCACATGCCAACATGGATATAATTCAACGATTAAGACACTTTACTCCTTCCTTTAAAGTCGGATAAAGTCGGAGGTTCGATATCCCATCCCTACATCTGTTGTACTAAAAAAATGATAAAAAAAAAGTGATTTTAAAAAATAGCAAAAATACCAAACATGGTCTTAATCGTTGAATTAAATTTTTTTCTTCTTCTGATACATCCCTGAAACTATTAATTTGCTGGTTTGATTAATGTTTGTGGTGGAAAATTGAATTGGTTATTGCAGGAAATGAAGTGTGTGATAGATTTGCAAGCACAGGGTTCCACGCCAACATAATTACATACTTGACCCAAGATCTTAACATGCCTCTTGTTCCTGCCTCCAACATTCTCACCAACTTCGCCGCCACTTCCAGCTTCACTTCCCTCATCGGAGCTCTCATCGCCGACTCCTTCGCCGGCCGCTTCTGGACCATCACCGTCGCCTCCATCATCTACCACCTCGTATCTCTCTTTCTCTCCACTCTTCTAATTGTCGATTGGTTTTGAAATAGAACCTATATTGATATCGTTTCAGAACTCAACAACCTAAAACGAATATCCATTTTTGAAAATGGCAAAAAAGAACCACTATCTTGAGAAGGGACGACTCGGAAAGTTAAGAATCCATTTGGTAACGTTCCCGTTTCTTGTTCTCATTTCCAAATTTTATTAAAAAACTTAAGCACTTTGCAATTAAATATATTTCTTGCTTCTCATTGTTTTGAAAACATTATCAAAATTGTATAGAAATTTTAGAAACAATCCAAACAAGTTTTTTGTTTCTTGTTTCCATTTCCAATTTGTTACCATACATGAATATAAAATTATTTGATCATTCTTGAATTAATGGGTGTGAGGGCTATAAAATTTTATTTTTCTTTATTAAAAATAAGAAACAGAAACAAGAACGTTATTAAACGAGTCCTAATCGATTAATAATTATACGTAATTAGTTAAAACTTATGGATTTAGTAATATTTTGTATTCTACAGGGAATGGCTACGATCACGGTCTCCGCCATTCTCCCGCAGCTCCACCCGACGCCTTGCCCGACCCAACTCAACTGCACGCAAGCCTCCGGCACGCAGCTGATGGTCTTCTACCTCGCCCTTCTCCTTACCTCTCTCGGCGCTGGCGGCATTCGGCCGTGCGTCGTCGCATTCGCAGCCGATCAGTTCGACATGACGAAGGTGGGCGTGGCCGGGAGAAGCTGGAACTTCTTCAATTGGTACTATTTCTGCATGGGGCTAGCCACCCTCACGGCCCTAACCGTGGTGGTCTATATCCAGGACAACGTGGGGTGGGGGTGGGGATTCGGGCTTCCGACCATTGCCATGGGCTTGTCAGTGGTGGCGTTCGTGGTCGGGTCGCCGCTATATAAGAAAATCAAACCCAGCGGCAGCCCGTTGGTTAGATTGGCTCAGGTTGTGGTGGCGGCTGTGAAGAATAGGAAGGCTGTGCTACCGGATGACCCGAAGCTGCTCTATAGGAACCACAAGCTTGATGCTGCCATTGCCTCTCAAGGAACGCTTGTCCATACAAATCAATTCAAGTAAGCTTAAATTTGATTATCTCGTATTCTATGGTTTATGAATTTCGAAAACTTTGAATTGCAACTTACGAATTTGGTTCTTTTTGGGTTGCATTACTTTTAAATTAGTTACTACTGATTTCTAATTTGGTCTTGAAGTTACATTTCGTTTGGTTCCAATAACTTTAAAAGTTTTAATTTGGTTATTATACAGTTTTAAGGATGTTATAGTTTCAATTTGTTATACTTGATTTCTTTAAAATAGAGAAAAATAAATATGTTAAAAGGTGTCGAGGAGCCAAGTTTTTTCCCTTGGCTCCATCACTCTTATTTTATCCAATTAATATATCTTTTAGTCAAATCATCATTCCAAAGTATTTTCCTATATTTTAATGCGTTAATTTCAGGTGGCTAGACAAGGCCGCTGTGGTAACCAACGGCGAAAGAGAGAGCCCACCAAACCTGTGGCGCGTGGCCACCGTCCATCGCGTAGAAGAGCTGAAGTCCATCATCCGCATGCTCCCAATATGGGCAGCCGGAATCCTGCTGGTGACTGCCTCCTCCCACCAGCACAGCTTCACAATCCAGCAAGCCCGCACCATGGATCGTCGCCTCTCAAACTCCTTCCAAATCCCTCCAGCCACCCTCTCCATCTTCGGCAACCTCTCGATGCTCACCGGCCTTGTCCTCTACGAGCGCCTCCTCATCCCCTTCGCCCGCCGCTTCACCAAAAACCCCTCCGGCATCACCTGCCTCCAGCGCATGGGTGTCGGCTTCGCCGTCAACATCCTCGCCACCCTCGTCTCCTCCCTGGTGGAGATCAGGCGGAAGAGGGTCGCCATGGAACATGGCTTGCTCGACCGCCCGACCGCCACCATCCCCTTCAGCGTCTTCTGGCTGGTGCCGCAGTTCTGGCTGCATGGGATCGCGGAGGTGTTCATGTCGGTGGGGCATTTGGAGTTCATGTACGACCAGTCGCCGGAGAGCTTGAGGAGCAGCGCCACGGCACTGTACTGGTTGGCCATCTCGGCTGGGAACTACATTGGGACTTTGATGGTGTATGTTGTGCATAAGTACAGTGGGGAGAAGCACAATTGGTTACCTGATAGGAATCTGAACAGAGGGAGGCTTGAGTATTATTATTGGGTTGTGAGTGGGATTCAAGTGCTGAATCTTGTGTATTATGTGGTTTGTGCTTGGTTTTATACTTACAAGCCTTTGGAGGAGGAGAGACAGAACAGTGATGGAGAGGGAGATCAGTGTGAAGATATCACGAAGACAGCTTAAGAGAGTTGTGTTTTGTGTTCTATGTAAGGGAAAATGTGGTAGCTAGGTTAGAGTTTTATAATTTGACTAGATTGCTTTACAAAGAAATTGTAACTTCTTCCCATCAAGCAAACTTCAGCTCTCTTAGTTATAATTACTTTTCATAAAGGTTGCATCACATGATTAAGATTTAGAAGTTTTATTAATAAC

mRNA sequence

TGTCAATTTTTGGAGGGCCCCACTTTCTGATTATGAGGATTATAAAACGGCACCGTATTGTGTGTCATTGGCCATATAAAGAGGGGTTATTGTGGAGGGAAGAAGTGGAGAGACAATGGAGATAGAATCCATTAGAGTGGGAGTTGATGAGAAAGAGAAGAAGAAATTAGAAGAAGAGATGGAATTAGAGGAAGGGGAGAAGAAGAAGGACAGGAAGCTTGGTGGAGTCAAGACAATGCCTTTCATTCTTGGAAATGAAGTGTGTGATAGATTTGCAAGCACAGGGTTCCACGCCAACATAATTACATACTTGACCCAAGATCTTAACATGCCTCTTGTTCCTGCCTCCAACATTCTCACCAACTTCGCCGCCACTTCCAGCTTCACTTCCCTCATCGGAGCTCTCATCGCCGACTCCTTCGCCGGCCGCTTCTGGACCATCACCGTCGCCTCCATCATCTACCACCTCGGAATGGCTACGATCACGGTCTCCGCCATTCTCCCGCAGCTCCACCCGACGCCTTGCCCGACCCAACTCAACTGCACGCAAGCCTCCGGCACGCAGCTGATGGTCTTCTACCTCGCCCTTCTCCTTACCTCTCTCGGCGCTGGCGGCATTCGGCCGTGCGTCGTCGCATTCGCAGCCGATCAGTTCGACATGACGAAGGTGGGCGTGGCCGGGAGAAGCTGGAACTTCTTCAATTGGTACTATTTCTGCATGGGGCTAGCCACCCTCACGGCCCTAACCGTGGTGGTCTATATCCAGGACAACGTGGGGTGGGGGTGGGGATTCGGGCTTCCGACCATTGCCATGGGCTTGTCAGTGGTGGCGTTCGTGGTCGGGTCGCCGCTATATAAGAAAATCAAACCCAGCGGCAGCCCGTTGGTTAGATTGGCTCAGGTTGTGGTGGCGGCTGTGAAGAATAGGAAGGCTGTGCTACCGGATGACCCGAAGCTGCTCTATAGGAACCACAAGCTTGATGCTGCCATTGCCTCTCAAGGAACGCTTGTCCATACAAATCAATTCAAGTGGCTAGACAAGGCCGCTGTGGTAACCAACGGCGAAAGAGAGAGCCCACCAAACCTGTGGCGCGTGGCCACCGTCCATCGCGTAGAAGAGCTGAAGTCCATCATCCGCATGCTCCCAATATGGGCAGCCGGAATCCTGCTGGTGACTGCCTCCTCCCACCAGCACAGCTTCACAATCCAGCAAGCCCGCACCATGGATCGTCGCCTCTCAAACTCCTTCCAAATCCCTCCAGCCACCCTCTCCATCTTCGGCAACCTCTCGATGCTCACCGGCCTTGTCCTCTACGAGCGCCTCCTCATCCCCTTCGCCCGCCGCTTCACCAAAAACCCCTCCGGCATCACCTGCCTCCAGCGCATGGGTGTCGGCTTCGCCGTCAACATCCTCGCCACCCTCGTCTCCTCCCTGGTGGAGATCAGGCGGAAGAGGGTCGCCATGGAACATGGCTTGCTCGACCGCCCGACCGCCACCATCCCCTTCAGCGTCTTCTGGCTGGTGCCGCAGTTCTGGCTGCATGGGATCGCGGAGGTGTTCATGTCGGTGGGGCATTTGGAGTTCATGTACGACCAGTCGCCGGAGAGCTTGAGGAGCAGCGCCACGGCACTGTACTGGTTGGCCATCTCGGCTGGGAACTACATTGGGACTTTGATGGTGTATGTTGTGCATAAGTACAGTGGGGAGAAGCACAATTGGTTACCTGATAGGAATCTGAACAGAGGGAGGCTTGAGTATTATTATTGGGTTGTGAGTGGGATTCAAGTGCTGAATCTTGTGTATTATGTGGTTTGTGCTTGGTTTTATACTTACAAGCCTTTGGAGGAGGAGAGACAGAACAGTGATGGAGAGGGAGATCAGTGTGAAGATATCACGAAGACAGCTTAAGAGAGTTGTGTTTTGTGTTCTATGTAAGGGAAAATGTGGTAGCTAGGTTAGAGTTTTATAATTTGACTAGATTGCTTTACAAAGAAATTGTAACTTCTTCCCATCAAGCAAACTTCAGCTCTCTTAGTTATAATTACTTTTCATAAAGGTTGCATCACATGATTAAGATTTAGAAGTTTTATTAATAAC

Coding sequence (CDS)

ATGGAGATAGAATCCATTAGAGTGGGAGTTGATGAGAAAGAGAAGAAGAAATTAGAAGAAGAGATGGAATTAGAGGAAGGGGAGAAGAAGAAGGACAGGAAGCTTGGTGGAGTCAAGACAATGCCTTTCATTCTTGGAAATGAAGTGTGTGATAGATTTGCAAGCACAGGGTTCCACGCCAACATAATTACATACTTGACCCAAGATCTTAACATGCCTCTTGTTCCTGCCTCCAACATTCTCACCAACTTCGCCGCCACTTCCAGCTTCACTTCCCTCATCGGAGCTCTCATCGCCGACTCCTTCGCCGGCCGCTTCTGGACCATCACCGTCGCCTCCATCATCTACCACCTCGGAATGGCTACGATCACGGTCTCCGCCATTCTCCCGCAGCTCCACCCGACGCCTTGCCCGACCCAACTCAACTGCACGCAAGCCTCCGGCACGCAGCTGATGGTCTTCTACCTCGCCCTTCTCCTTACCTCTCTCGGCGCTGGCGGCATTCGGCCGTGCGTCGTCGCATTCGCAGCCGATCAGTTCGACATGACGAAGGTGGGCGTGGCCGGGAGAAGCTGGAACTTCTTCAATTGGTACTATTTCTGCATGGGGCTAGCCACCCTCACGGCCCTAACCGTGGTGGTCTATATCCAGGACAACGTGGGGTGGGGGTGGGGATTCGGGCTTCCGACCATTGCCATGGGCTTGTCAGTGGTGGCGTTCGTGGTCGGGTCGCCGCTATATAAGAAAATCAAACCCAGCGGCAGCCCGTTGGTTAGATTGGCTCAGGTTGTGGTGGCGGCTGTGAAGAATAGGAAGGCTGTGCTACCGGATGACCCGAAGCTGCTCTATAGGAACCACAAGCTTGATGCTGCCATTGCCTCTCAAGGAACGCTTGTCCATACAAATCAATTCAAGTGGCTAGACAAGGCCGCTGTGGTAACCAACGGCGAAAGAGAGAGCCCACCAAACCTGTGGCGCGTGGCCACCGTCCATCGCGTAGAAGAGCTGAAGTCCATCATCCGCATGCTCCCAATATGGGCAGCCGGAATCCTGCTGGTGACTGCCTCCTCCCACCAGCACAGCTTCACAATCCAGCAAGCCCGCACCATGGATCGTCGCCTCTCAAACTCCTTCCAAATCCCTCCAGCCACCCTCTCCATCTTCGGCAACCTCTCGATGCTCACCGGCCTTGTCCTCTACGAGCGCCTCCTCATCCCCTTCGCCCGCCGCTTCACCAAAAACCCCTCCGGCATCACCTGCCTCCAGCGCATGGGTGTCGGCTTCGCCGTCAACATCCTCGCCACCCTCGTCTCCTCCCTGGTGGAGATCAGGCGGAAGAGGGTCGCCATGGAACATGGCTTGCTCGACCGCCCGACCGCCACCATCCCCTTCAGCGTCTTCTGGCTGGTGCCGCAGTTCTGGCTGCATGGGATCGCGGAGGTGTTCATGTCGGTGGGGCATTTGGAGTTCATGTACGACCAGTCGCCGGAGAGCTTGAGGAGCAGCGCCACGGCACTGTACTGGTTGGCCATCTCGGCTGGGAACTACATTGGGACTTTGATGGTGTATGTTGTGCATAAGTACAGTGGGGAGAAGCACAATTGGTTACCTGATAGGAATCTGAACAGAGGGAGGCTTGAGTATTATTATTGGGTTGTGAGTGGGATTCAAGTGCTGAATCTTGTGTATTATGTGGTTTGTGCTTGGTTTTATACTTACAAGCCTTTGGAGGAGGAGAGACAGAACAGTGATGGAGAGGGAGATCAGTGTGAAGATATCACGAAGACAGCTTAA

Protein sequence

MEIESIRVGVDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDKAAVVTNGERESPPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNWLPDRNLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEERQNSDGEGDQCEDITKTA
Homology
BLAST of MC05g1040 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 3.6e-191
Identity = 327/580 (56.38%), Postives = 430/580 (74.14%), Query Frame = 0

Query: 10  VDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQD 69
           ++E+ K K+ EE +   G  + +R  GG+ TMPFI  NE+C++ A  GFHAN+I+YLT  
Sbjct: 1   MEEQSKNKISEEEKQLHG--RPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQ 60

Query: 70  LNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAIL 129
           L++PL  A+N LTNFA TSS T L+GA IADSFAGRFWTIT ASIIY +GM  +T+SAI+
Sbjct: 61  LHLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAII 120

Query: 130 PQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAG 189
           P L P PC  +  C  A   QL + Y+ALLL +LG+GGIRPCVVAF ADQFD +      
Sbjct: 121 PTLRPPPCKGEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTT 180

Query: 190 RSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKK 249
           ++WN+FNWYYFCMG A L A+TV+V+IQDNVGWG G G+PT+AM LSV+AFV G  LY+ 
Sbjct: 181 KTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRH 240

Query: 250 IKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDK 309
           + P+GSP  RL QV VAA + RK  +  DP LLY N ++DA I+  G L HT    +LDK
Sbjct: 241 LVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDK 300

Query: 310 AAVVTNGERESP---PNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQ 369
           AA+VT  +   P   PN WR++TVHRVEELKS+IRM PI A+GILL+TA + Q +F++QQ
Sbjct: 301 AAIVTEEDNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQ 360

Query: 370 ARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGV 429
           A+TM+R L+NSFQIP  ++S+F  ++MLT ++ Y+R+ +  AR+FT    GIT L RMG+
Sbjct: 361 AKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGI 420

Query: 430 GFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVG 489
           GF ++I+ATLV+  VE++RK VA+EHGLLD+P   +P S  WL+PQ+ LHG+AE FMS+G
Sbjct: 421 GFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIG 480

Query: 490 HLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSG--EKHNWLPDRNLNRG 549
           HLEF YDQ+PES+RS+ATAL+W+AIS GNY+ TL+V +VHK+S   +  NWLPD NLNRG
Sbjct: 481 HLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRG 540

Query: 550 RLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEERQNSD 585
           RLEY+YW+++ +Q +NLVYY+ CA  YTYKP++      D
Sbjct: 541 RLEYFYWLITVLQAVNLVYYLWCAKIYTYKPVQVHHSKED 578

BLAST of MC05g1040 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 2.3e-121
Identity = 229/548 (41.79%), Postives = 351/548 (64.05%), Query Frame = 0

Query: 27  GEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAA 86
           G     +K G  K  PFILGNE C+R A  G   N+ITY T +L+   V A++ +  +  
Sbjct: 45  GNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQG 104

Query: 87  TSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAILPQLHPTPCP--TQLNCT 146
           T   T LIGA+IADS+ GR+WTI   S IY +GMA +T+SA LP L P  C       C+
Sbjct: 105 TCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCS 164

Query: 147 QASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRSWNFFNWYYFCMGL 206
            A+  Q  VF+  L L +LG GGI+PCV +F ADQFD T      R  +FFNW+YF + +
Sbjct: 165 PATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINI 224

Query: 207 ATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKKIKPSGSPLVRLAQVV 266
            +  + T++V++Q+NVGWG GF +PT+ MG+S+ +F +G+PLY+  KP GSP+ R+ QV+
Sbjct: 225 GSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVL 284

Query: 267 VAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDKAAVVTNGERESP--P 326
           VAA +  K  LP+D   LY   + ++ IA    + HT+ +K+LDKAAV++  E +S    
Sbjct: 285 VAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYESKSGAFS 344

Query: 327 NLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMDRRLSNSFQIPP 386
           N W++ TV +VEE+K++IRM PIWA+GI+     S   +  +QQ R+M+ R+  SF+IPP
Sbjct: 345 NPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMN-RIIRSFEIPP 404

Query: 387 ATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGVGFAVNILATLVSSLVE 446
           A+  +F  L +L  + +Y+R L+PF RRFT  P G+T LQRMG+G  +++L+   +++VE
Sbjct: 405 ASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVE 464

Query: 447 IRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSS 506
             R ++A +          +  S+FW +PQ+ L GIAEVF  +G +EF YD+SP+++RS 
Sbjct: 465 TVRLQLAQDF---------VAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSV 524

Query: 507 ATALYWLAISAGNYIGTLMVYVVHKYS--GEKHNWLPDRNLNRGRLEYYYWVVSGIQVLN 566
            +AL  L  + G+Y+ +L++ +V  ++  G K  W+PD +LN+G L+Y++W++  + ++N
Sbjct: 525 CSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPD-DLNKGHLDYFFWLLVSLGLVN 581

Query: 567 L-VYYVVC 568
           + VY ++C
Sbjct: 585 IPVYALIC 581

BLAST of MC05g1040 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 3.0e-121
Identity = 233/568 (41.02%), Postives = 355/568 (62.50%), Query Frame = 0

Query: 11  DEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQDL 70
           D+K+    +  +++ +    K+ K G  K   FILG E C+R A  G   N+I YL + +
Sbjct: 3   DDKDIYTKDGTLDIHKKPANKN-KTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQM 62

Query: 71  NMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAILP 130
           NM  V AS  ++N++ T   T LIGA IAD++ GR+WTI    +IY  GM  +T+SA +P
Sbjct: 63  NMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVP 122

Query: 131 QLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGR 190
            L PT C  +  C   +G Q  + ++AL L +LG GGI+PCV +F ADQFD T       
Sbjct: 123 GLTPT-CSGE-TCHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKES 182

Query: 191 SWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKKI 250
             +FFNW+YF + +  + A +V+V+IQ NVGWGWG G+PT+AM ++VV F  GS  Y+  
Sbjct: 183 KSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQ 242

Query: 251 KPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDKA 310
           KP GSPL R+ QV+VA+ +  K  +P+D  LLY N   +++I     L HT    + DKA
Sbjct: 243 KPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKA 302

Query: 311 AVVTNGERE--SPPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQAR 370
           AV T  + +  +  + W++ TV +VEELK++IR+LPIWA GI+  +  S   +  + Q  
Sbjct: 303 AVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGN 362

Query: 371 TMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGVGF 430
           T+D+ +  +F+IP A+LS+F  LS+L    +Y++L++PFAR++T +  G T LQR+G+G 
Sbjct: 363 TLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGL 422

Query: 431 AVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVGHL 490
            ++I + + + ++E+ R      H L +    TIP ++FW VPQ++L G AEVF  +G L
Sbjct: 423 VISIFSMVSAGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQL 482

Query: 491 EFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKY--SGEKHNWLPDRNLNRGRL 550
           EF YDQ+P+++RS  +AL   AI+ GNY+ T +V +V K   SG +  W+  +NLN G L
Sbjct: 483 EFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIA-KNLNNGHL 542

Query: 551 EYYYWVVSGIQVLNLVYYVVCAWFYTYK 575
           +Y++W+++G+  LN + Y+  A +YTYK
Sbjct: 543 DYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

BLAST of MC05g1040 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 3.3e-120
Identity = 235/582 (40.38%), Postives = 357/582 (61.34%), Query Frame = 0

Query: 10  VDEKEKKKLEEEMELEE-------------GEKKKDRKLGGVKTMPFILGNEVCDRFAST 69
           ++E+ +  +EE + L+E             G      K G  K  PFILGNE C+R A  
Sbjct: 4   IEEEARPLIEEGLILQEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYY 63

Query: 70  GFHANIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIY 129
           G   N+ITYLT  L+   V A+  +T +  T   T LIGA++AD++ GR+WTI   S IY
Sbjct: 64  GIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIY 123

Query: 130 HLGMATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFA 189
            +GM+ +T+SA +P L P  C     C  A+  Q  +F+  L L +LG GGI+PCV +F 
Sbjct: 124 FIGMSALTLSASVPALKPAECIGDF-CPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFG 183

Query: 190 ADQFDMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLS 249
           ADQFD T      R  +FFNW+YF + +  L + +++V+IQ+N GWG GFG+PT+ MGL+
Sbjct: 184 ADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLA 243

Query: 250 VVAFVVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQG 309
           + +F  G+PLY+  KP GSP+ R++QVVVA+ +     +P+D  LLY     ++AIA   
Sbjct: 244 IASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSR 303

Query: 310 TLVHTNQFKWLDKAAVVTNGERESP--PNLWRVATVHRVEELKSIIRMLPIWAAGILLVT 369
            + HT+  ++LDKAAV++  E +S    N WR+ TV +VEELK +IRM PIWA+GI+   
Sbjct: 304 KIEHTDDCQYLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSA 363

Query: 370 ASSHQHSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKN 429
             +   +  +QQ R M+ ++  SFQ+PPA L  F   S++  + LY+R ++P AR+FT  
Sbjct: 364 VYAQMSTMFVQQGRAMNCKI-GSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGV 423

Query: 430 PSGITCLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFW 489
             G T +QRMG+G  V++L    +++VEI R  +A + GL++   A +P SV W +PQ++
Sbjct: 424 DKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVE-SGAPVPISVLWQIPQYF 483

Query: 490 LHGIAEVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYS--GEK 549
           + G AEVF  +G LEF YDQSP+++RS  +AL  L  + GNY+ +L++ +V  ++    +
Sbjct: 484 ILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQ 543

Query: 550 HNWLPDRNLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYK 575
             W+ D NLN G L+Y++W+++G+ ++N+  Y   A  Y  K
Sbjct: 544 EGWISD-NLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of MC05g1040 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.8e-119
Identity = 231/569 (40.60%), Postives = 347/569 (60.98%), Query Frame = 0

Query: 10  VDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQD 69
           ++EK+    +  +++ +    K+ K G  K   FILGNE C+R A  G   N++ YL   
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKE-KTGNWKACRFILGNECCERLAYYGMGTNLVNYLESR 60

Query: 70  LNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAIL 129
           LN     A+N +TN++ T   T LIGA IAD++ GR+WTI     IY  GM  +T+SA +
Sbjct: 61  LNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASV 120

Query: 130 PQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAG 189
           P L P  C    +    + +Q  VF++AL + +LG GGI+PCV +F ADQFD        
Sbjct: 121 PGLKPGNC--NADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKI 180

Query: 190 RSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKK 249
           +  +FFNW+YF + +  L A TV+V+IQ NVGWGWGFG+PT+AM ++V  F  GS  Y+ 
Sbjct: 181 KKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRL 240

Query: 250 IKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDK 309
            +P GSPL R+ QV+VAA +     +P+D  LL+     ++ I     LVHT+  K+ DK
Sbjct: 241 QRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDK 300

Query: 310 AAVVTNGE--RESPPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQA 369
           AAV +  +  ++   N WR+ +V +VEELKSII +LP+WA GI+  T  S   +  + Q 
Sbjct: 301 AAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQG 360

Query: 370 RTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGVG 429
            TMD+ +  +F+IP A+LS+F  +S+L    +Y++ +IP AR+FT+N  G T LQRMG+G
Sbjct: 361 NTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIG 420

Query: 430 FAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVGH 489
             V+I A + + ++E+ R      H   D+    I  S+FW +PQ+ L G AEVF  +G 
Sbjct: 421 LVVSIFAMITAGVLEVVRLDYVKTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQ 480

Query: 490 LEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGE--KHNWLPDRNLNRGR 549
           LEF YDQ+P+++RS  +AL    ++ GNY+ T++V VV K + +  K  W+PD NLNRG 
Sbjct: 481 LEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGH 540

Query: 550 LEYYYWVVSGIQVLNLVYYVVCAWFYTYK 575
           L+Y++++++ +  LN + Y+  +  Y YK
Sbjct: 541 LDYFFYLLATLSFLNFLVYLWISKRYKYK 563

BLAST of MC05g1040 vs. NCBI nr
Match: NP_001267552.1 (probable nitrite transporter At1g68570-like [Cucumis sativus] >KGN59416.1 hypothetical protein Csa_000876 [Cucumis sativus] >CAA93316.2 nitrite transporter [Cucumis sativus])

HSP 1 Score: 1028 bits (2659), Expect = 0.0
Identity = 515/607 (84.84%), Postives = 560/607 (92.26%), Query Frame = 0

Query: 1   MEIESIRVGVDEK----EKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFAST 60
           MEIES+RV VDEK    E+K  +EEM+++    K  RKLGGVKTMPFILGNEVCDRFAS+
Sbjct: 1   MEIESLRVSVDEKVLKDEEKNEKEEMKMK---MKMKRKLGGVKTMPFILGNEVCDRFASS 60

Query: 61  GFHANIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIY 120
           GFH+NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTIT+ASIIY
Sbjct: 61  GFHSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIY 120

Query: 121 HLGMATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFA 180
            LGM TIT+SAILP LHP PCPTQ+NCTQASGTQLM+ YLALLLTSLGAGGIRPCVVAFA
Sbjct: 121 ELGMVTITISAILPSLHPPPCPTQINCTQASGTQLMILYLALLLTSLGAGGIRPCVVAFA 180

Query: 181 ADQFDMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLS 240
           ADQFDMTKVG+AGR+WNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFGLPTIAM LS
Sbjct: 181 ADQFDMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALS 240

Query: 241 VVAFVVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQG 300
           VVAFVVGSPLY K+KPSGSPLVRLAQVVVAA KNRKAVLPDD KLLYRNH+LDAAIA QG
Sbjct: 241 VVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPDDSKLLYRNHELDAAIAIQG 300

Query: 301 TLVHTNQFKWLDKAAVVTNGERES-PPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTA 360
            LVHT+QFKWLDKAAV+T+ +  + PPNLWR+ATVHRVEELKSIIRMLPIWAAGILLVTA
Sbjct: 301 RLVHTDQFKWLDKAAVITSPDSTANPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTA 360

Query: 361 SSHQHSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNP 420
           SSHQHSFTIQQARTM+R L+ +FQIPPATLSIFG LSMLTGLVLY+RLL+PFA++ T NP
Sbjct: 361 SSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTHNP 420

Query: 421 SGITCLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWL 480
           SGITCLQRMGVGFA+NILATLVSS+VEI+RK+VA  HGLLD PTATIPFSVFWLVPQFWL
Sbjct: 421 SGITCLQRMGVGFAINILATLVSSIVEIKRKKVAANHGLLDNPTATIPFSVFWLVPQFWL 480

Query: 481 HGIAEVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNW 540
           HGIAEVFMSVGHLEFMYDQSPESLRS+ATALYWLAIS GNYIGTLMVY+VHKY+GE+HNW
Sbjct: 481 HGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYIGTLMVYLVHKYTGEEHNW 540

Query: 541 LPDRNLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEER---QNSDG--EGDQC 597
           LPDRNLNRGRLEYYYW+VSGIQV+NLVYYV+CAWFYTYKPLEEE+   +N DG  E ++C
Sbjct: 541 LPDRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKINIENKDGDQEWERC 600

BLAST of MC05g1040 vs. NCBI nr
Match: XP_038903971.1 (protein NRT1/ PTR FAMILY 3.1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1020 bits (2637), Expect = 0.0
Identity = 511/603 (84.74%), Postives = 555/603 (92.04%), Query Frame = 0

Query: 1   MEIESIRVGVDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHA 60
           MEIES+RV VDEK    L +E + E+ E KK ++LGGVKTMPFILGNEVCDRFAS+GFHA
Sbjct: 1   MEIESLRVSVDEK---ILRDEEKDEKEEIKKKKQLGGVKTMPFILGNEVCDRFASSGFHA 60

Query: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGM 120
           NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSF GRFWTITVASIIY LGM
Sbjct: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFTGRFWTITVASIIYELGM 120

Query: 121 ATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQF 180
           ATIT+SAILP LHP PCPTQLNCTQASG QLMV YL+LLLTSLGAGGIRPCVVAFAADQF
Sbjct: 121 ATITISAILPSLHPPPCPTQLNCTQASGMQLMVLYLSLLLTSLGAGGIRPCVVAFAADQF 180

Query: 181 DMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAF 240
           DMTKVG+AGR+WNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFGLPTIAM LSVVAF
Sbjct: 181 DMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 240

Query: 241 VVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVH 300
           VVGSPLY K+KP GSPLVRLAQVVVAAVKNRKA+LP+D KLLYRNHKLDAAIA QG LVH
Sbjct: 241 VVGSPLYNKLKPGGSPLVRLAQVVVAAVKNRKAILPNDSKLLYRNHKLDAAIAVQGRLVH 300

Query: 301 TNQFKWLDKAAVVTNGERESP-PNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
           T+QFKWLDKAAV+T  +  +  PNLWR+ATVHRVEELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 301 TDQFKWLDKAAVITTPDSTTDQPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360

Query: 361 HSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGIT 420
           HSFTIQQARTM+R L+ +FQIPPATLSIFG +SMLTGL+LY+RLL+PFA++ T NPSGIT
Sbjct: 361 HSFTIQQARTMNRHLTPTFQIPPATLSIFGIISMLTGLILYDRLLVPFAKKLTNNPSGIT 420

Query: 421 CLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFA+NILATLVSS+VEI+RK+VA  HGLLD PTATIPFSVFWLVPQFWLHGIA
Sbjct: 421 CLQRMGVGFAINILATLVSSIVEIKRKKVAALHGLLDNPTATIPFSVFWLVPQFWLHGIA 480

Query: 481 EVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRS+ATALYWLAIS GNYIGTLMVY+VHKY+GE+HNWLPDR
Sbjct: 481 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYIGTLMVYLVHKYTGEEHNWLPDR 540

Query: 541 NLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEER----QNSDGEGDQCED-IT 597
           NLNRGRLEYYYW+VSGIQV+NLVYYV+CAWFYTYKPLEEE+     N D E ++CE+ I 
Sbjct: 541 NLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKIIENNNGDQEEERCEETII 600

BLAST of MC05g1040 vs. NCBI nr
Match: KAG6596175.1 (Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1018 bits (2632), Expect = 0.0
Identity = 516/607 (85.01%), Postives = 553/607 (91.10%), Query Frame = 0

Query: 1   MEIESIRVGVDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHA 60
           ME+ES+ VG D+ EK   E++    E E KK+RKLGGVKTMPFILGNE+CDRFASTGFHA
Sbjct: 5   MEMESLSVGADDAEKGMAEKQ----EMETKKERKLGGVKTMPFILGNEICDRFASTGFHA 64

Query: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGM 120
           NIITYLTQDLNMPLVPASNILTNFAATSSFT+LIGALIADSFAGRFWTIT ASIIY LGM
Sbjct: 65  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 124

Query: 121 ATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQF 180
           AT+T+SAI+P+LHP PCPTQLNC QAS TQL V YLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 125 ATVTISAIIPRLHPPPCPTQLNCIQASTTQLTVLYLALLLTSLGAGGIRPCVVAFAADQF 184

Query: 181 DMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAF 240
           DMTKVGVAGR+WNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAM LSVVAF
Sbjct: 185 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 244

Query: 241 VVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVH 300
           VVGSPLY K+KPSGSPLVRLAQVVVAAVK RKA LP+DPKLLYRNHKLDAAIA QG LVH
Sbjct: 245 VVGSPLYNKLKPSGSPLVRLAQVVVAAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 304

Query: 301 TNQFKWLDKAAVVTNGERES-PPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
           T+QFKWLDKAAV+T  E  S PPNLWR+ATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 305 TDQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 364

Query: 361 HSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGIT 420
           HSFT+QQARTM+R L+ +F+IPPA+LSIFG LSMLTGLVLYERLL+PFARRFTKNPSGIT
Sbjct: 365 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 424

Query: 421 CLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFA+NILATLVSSL+EIRRK+VA EH LLD PTATIPFSVFWLVPQFWLHGIA
Sbjct: 425 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 484

Query: 481 EVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRS+ATALYWLAIS GNY+GTLMV +VHKYSG++HNWLPDR
Sbjct: 485 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 544

Query: 541 NLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEERQNSDGEG---------DQC 597
           NLNRGRLE YYW+VSGIQVLNLVYYVVCAWFYTYKPLEEE+   D EG         D C
Sbjct: 545 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEK---DDEGELSSSTLSKDGC 603

BLAST of MC05g1040 vs. NCBI nr
Match: KAG7027713.1 (Protein NRT1/ PTR FAMILY 3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1018 bits (2632), Expect = 0.0
Identity = 516/607 (85.01%), Postives = 553/607 (91.10%), Query Frame = 0

Query: 1   MEIESIRVGVDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHA 60
           ME+ES+ VG D+ EK   E++    E E KK+RKLGGVKTMPFILGNE+CDRFASTGFHA
Sbjct: 1   MEMESLSVGADDAEKGMAEKQ----EMETKKERKLGGVKTMPFILGNEICDRFASTGFHA 60

Query: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGM 120
           NIITYLTQDLNMPLVPASNILTNFAATSSFT+LIGALIADSFAGRFWTIT ASIIY LGM
Sbjct: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 120

Query: 121 ATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQF 180
           AT+T+SAI+P+LHP PCPTQLNC QAS TQL V YLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 121 ATVTISAIIPRLHPPPCPTQLNCIQASTTQLTVLYLALLLTSLGAGGIRPCVVAFAADQF 180

Query: 181 DMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAF 240
           DMTKVGVAGR+WNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAM LSVVAF
Sbjct: 181 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 240

Query: 241 VVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVH 300
           VVGSPLY K+KPSGSPLVRLAQVVVAAVK RKA LP+DPKLLYRNHKLDAAIA QG LVH
Sbjct: 241 VVGSPLYNKLKPSGSPLVRLAQVVVAAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 300

Query: 301 TNQFKWLDKAAVVTNGERES-PPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
           T+QFKWLDKAAV+T  E  S PPNLWR+ATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 301 TDQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 360

Query: 361 HSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGIT 420
           HSFT+QQARTM+R L+ +F+IPPA+LSIFG LSMLTGLVLYERLL+PFARRFTKNPSGIT
Sbjct: 361 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 420

Query: 421 CLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFA+NILATLVSSL+EIRRK+VA EH LLD PTATIPFSVFWLVPQFWLHGIA
Sbjct: 421 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 480

Query: 481 EVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRS+ATALYWLAIS GNY+GTLMV +VHKYSG++HNWLPDR
Sbjct: 481 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 540

Query: 541 NLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEERQNSDGEG---------DQC 597
           NLNRGRLE YYW+VSGIQVLNLVYYVVCAWFYTYKPLEEE+   D EG         D C
Sbjct: 541 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEK---DDEGELSSSTLSKDGC 599

BLAST of MC05g1040 vs. NCBI nr
Match: XP_022971207.1 (protein NRT1/ PTR FAMILY 3.1-like [Cucurbita maxima])

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 515/605 (85.12%), Postives = 554/605 (91.57%), Query Frame = 0

Query: 1   MEIESIRVGVDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHA 60
           ME+ES+ V  D +  K + E+ E+E    +K+RKLGGVKTMPFILGNE+CDRFASTGFHA
Sbjct: 5   MEMESLSVSADAE--KGMAEKQEMET---RKERKLGGVKTMPFILGNEICDRFASTGFHA 64

Query: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGM 120
           NIITYLTQDLNMPLVPASNILTNFAATSSFT+LIGALIADSFAGRFWTIT ASIIY LGM
Sbjct: 65  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 124

Query: 121 ATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQF 180
           ATIT+SAI+P+LHP PCPTQLNC QAS TQL VFYLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 125 ATITISAIIPRLHPPPCPTQLNCIQASSTQLTVFYLALLLTSLGAGGIRPCVVAFAADQF 184

Query: 181 DMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAF 240
           DMTKVGVAGR+WNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAM LSVVAF
Sbjct: 185 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 244

Query: 241 VVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVH 300
           VVGSPLY K+KPSGSPLVRLAQVVVAAVK RKA LP+DPKLLYRNHKLDAAIA QG LVH
Sbjct: 245 VVGSPLYNKLKPSGSPLVRLAQVVVAAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 304

Query: 301 TNQFKWLDKAAVVTNGERES-PPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
           T+QFKWLDKAAV+T  E  S PPNLWR+ATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 305 TDQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 364

Query: 361 HSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGIT 420
           HSFT+QQARTM+R L+ +F+IPPA+LSIFG LSMLTGLVLYERLL+PFARRFTKNPSGIT
Sbjct: 365 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 424

Query: 421 CLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFA+NILATLVSSL+EIRRK+VA EH LLD PTATIPFSVFWLVPQFWLHGIA
Sbjct: 425 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 484

Query: 481 EVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRS+ATALYWLAIS GNY+GTLMV +VHKYSG++HNWLPDR
Sbjct: 485 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 544

Query: 541 NLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEERQNSDGEG-------DQCED 597
           NLNRGRLE YYW+VSGIQVLNLVYYVVCAWFYTYKPLEEE+ + +GE        D CED
Sbjct: 545 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEKDD-EGEPSSSTLSKDGCED 602

BLAST of MC05g1040 vs. ExPASy TrEMBL
Match: Q96400 (Nitrite transporter OS=Cucumis sativus OX=3659 GN=NiTR1 PE=2 SV=2)

HSP 1 Score: 1028 bits (2659), Expect = 0.0
Identity = 515/607 (84.84%), Postives = 560/607 (92.26%), Query Frame = 0

Query: 1   MEIESIRVGVDEK----EKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFAST 60
           MEIES+RV VDEK    E+K  +EEM+++    K  RKLGGVKTMPFILGNEVCDRFAS+
Sbjct: 1   MEIESLRVSVDEKVLKDEEKNEKEEMKMK---MKMKRKLGGVKTMPFILGNEVCDRFASS 60

Query: 61  GFHANIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIY 120
           GFH+NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTIT+ASIIY
Sbjct: 61  GFHSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIY 120

Query: 121 HLGMATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFA 180
            LGM TIT+SAILP LHP PCPTQ+NCTQASGTQLM+ YLALLLTSLGAGGIRPCVVAFA
Sbjct: 121 ELGMVTITISAILPSLHPPPCPTQINCTQASGTQLMILYLALLLTSLGAGGIRPCVVAFA 180

Query: 181 ADQFDMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLS 240
           ADQFDMTKVG+AGR+WNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFGLPTIAM LS
Sbjct: 181 ADQFDMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALS 240

Query: 241 VVAFVVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQG 300
           VVAFVVGSPLY K+KPSGSPLVRLAQVVVAA KNRKAVLPDD KLLYRNH+LDAAIA QG
Sbjct: 241 VVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPDDSKLLYRNHELDAAIAIQG 300

Query: 301 TLVHTNQFKWLDKAAVVTNGERES-PPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTA 360
            LVHT+QFKWLDKAAV+T+ +  + PPNLWR+ATVHRVEELKSIIRMLPIWAAGILLVTA
Sbjct: 301 RLVHTDQFKWLDKAAVITSPDSTANPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTA 360

Query: 361 SSHQHSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNP 420
           SSHQHSFTIQQARTM+R L+ +FQIPPATLSIFG LSMLTGLVLY+RLL+PFA++ T NP
Sbjct: 361 SSHQHSFTIQQARTMNRHLTPTFQIPPATLSIFGILSMLTGLVLYDRLLVPFAKKLTHNP 420

Query: 421 SGITCLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWL 480
           SGITCLQRMGVGFA+NILATLVSS+VEI+RK+VA  HGLLD PTATIPFSVFWLVPQFWL
Sbjct: 421 SGITCLQRMGVGFAINILATLVSSIVEIKRKKVAANHGLLDNPTATIPFSVFWLVPQFWL 480

Query: 481 HGIAEVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNW 540
           HGIAEVFMSVGHLEFMYDQSPESLRS+ATALYWLAIS GNYIGTLMVY+VHKY+GE+HNW
Sbjct: 481 HGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISVGNYIGTLMVYLVHKYTGEEHNW 540

Query: 541 LPDRNLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEER---QNSDG--EGDQC 597
           LPDRNLNRGRLEYYYW+VSGIQV+NLVYYV+CAWFYTYKPLEEE+   +N DG  E ++C
Sbjct: 541 LPDRNLNRGRLEYYYWLVSGIQVMNLVYYVICAWFYTYKPLEEEKINIENKDGDQEWERC 600

BLAST of MC05g1040 vs. ExPASy TrEMBL
Match: A0A6J1I1C5 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita maxima OX=3661 GN=LOC111469999 PE=3 SV=1)

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 515/605 (85.12%), Postives = 554/605 (91.57%), Query Frame = 0

Query: 1   MEIESIRVGVDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHA 60
           ME+ES+ V  D +  K + E+ E+E    +K+RKLGGVKTMPFILGNE+CDRFASTGFHA
Sbjct: 5   MEMESLSVSADAE--KGMAEKQEMET---RKERKLGGVKTMPFILGNEICDRFASTGFHA 64

Query: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGM 120
           NIITYLTQDLNMPLVPASNILTNFAATSSFT+LIGALIADSFAGRFWTIT ASIIY LGM
Sbjct: 65  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 124

Query: 121 ATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQF 180
           ATIT+SAI+P+LHP PCPTQLNC QAS TQL VFYLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 125 ATITISAIIPRLHPPPCPTQLNCIQASSTQLTVFYLALLLTSLGAGGIRPCVVAFAADQF 184

Query: 181 DMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAF 240
           DMTKVGVAGR+WNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAM LSVVAF
Sbjct: 185 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 244

Query: 241 VVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVH 300
           VVGSPLY K+KPSGSPLVRLAQVVVAAVK RKA LP+DPKLLYRNHKLDAAIA QG LVH
Sbjct: 245 VVGSPLYNKLKPSGSPLVRLAQVVVAAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 304

Query: 301 TNQFKWLDKAAVVTNGERES-PPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
           T+QFKWLDKAAV+T  E  S PPNLWR+ATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 305 TDQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 364

Query: 361 HSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGIT 420
           HSFT+QQARTM+R L+ +F+IPPA+LSIFG LSMLTGLVLYERLL+PFARRFTKNPSGIT
Sbjct: 365 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 424

Query: 421 CLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFA+NILATLVSSL+EIRRK+VA EH LLD PTATIPFSVFWLVPQFWLHGIA
Sbjct: 425 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 484

Query: 481 EVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRS+ATALYWLAIS GNY+GTLMV +VHKYSG++HNWLPDR
Sbjct: 485 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 544

Query: 541 NLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEERQNSDGEG-------DQCED 597
           NLNRGRLE YYW+VSGIQVLNLVYYVVCAWFYTYKPLEEE+ + +GE        D CED
Sbjct: 545 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEKDD-EGEPSSSTLSKDGCED 602

BLAST of MC05g1040 vs. ExPASy TrEMBL
Match: A0A6J1FLW3 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita moschata OX=3662 GN=LOC111446603 PE=3 SV=1)

HSP 1 Score: 1012 bits (2617), Expect = 0.0
Identity = 509/589 (86.42%), Postives = 543/589 (92.19%), Query Frame = 0

Query: 1   MEIESIRVGVDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHA 60
           ME+ES  V  D+ EK   E++    E E KK+RKLGGVKTMPFILGNE+CDRFASTGFHA
Sbjct: 5   MEMESSSVRADDAEKGMAEKQ----EMETKKERKLGGVKTMPFILGNEICDRFASTGFHA 64

Query: 61  NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGM 120
           NIITYLTQDLNMPLVPASNILTNFAATSSFT+LIGALIADSFAGRFWTIT ASIIY LGM
Sbjct: 65  NIITYLTQDLNMPLVPASNILTNFAATSSFTALIGALIADSFAGRFWTITFASIIYELGM 124

Query: 121 ATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQF 180
           ATIT+SAI+P+LHP PCPTQLNC QAS TQL V YLALLLTSLGAGGIRPCVVAFAADQF
Sbjct: 125 ATITISAIIPRLHPPPCPTQLNCIQASTTQLTVLYLALLLTSLGAGGIRPCVVAFAADQF 184

Query: 181 DMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAF 240
           DMTKVGVAGR+WNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAM LSVVAF
Sbjct: 185 DMTKVGVAGRTWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAF 244

Query: 241 VVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVH 300
           VVGSPLY K+KPSGSPLVRLAQVVV AVK RKA LP+DPKLLYRNHKLDAAIA QG LVH
Sbjct: 245 VVGSPLYNKLKPSGSPLVRLAQVVVTAVKKRKASLPEDPKLLYRNHKLDAAIAIQGRLVH 304

Query: 301 TNQFKWLDKAAVVTNGERES-PPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQ 360
           TNQFKWLDKAAV+T  E  S PPNLWR+ATVHR+EELKSIIRMLPIWAAGILLVTASSHQ
Sbjct: 305 TNQFKWLDKAAVITTDESTSDPPNLWRLATVHRIEELKSIIRMLPIWAAGILLVTASSHQ 364

Query: 361 HSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGIT 420
           HSFT+QQARTM+R L+ +F+IPPA+LSIFG LSMLTGLVLYERLL+PFARRFTKNPSGIT
Sbjct: 365 HSFTVQQARTMNRHLTPTFEIPPASLSIFGILSMLTGLVLYERLLVPFARRFTKNPSGIT 424

Query: 421 CLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIA 480
           CLQRMGVGFA+NILATLVSSL+EIRRK+VA EH LLD PTATIPFSVFWLVPQFWLHGIA
Sbjct: 425 CLQRMGVGFAINILATLVSSLIEIRRKKVAAEHNLLDDPTATIPFSVFWLVPQFWLHGIA 484

Query: 481 EVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNWLPDR 540
           EVFMSVGHLEFMYDQSPESLRS+ATALYWLAIS GNY+GTLMV +VHKYSG++HNWLPDR
Sbjct: 485 EVFMSVGHLEFMYDQSPESLRSTATALYWLAISMGNYVGTLMVSLVHKYSGKEHNWLPDR 544

Query: 541 NLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEERQNSDGEGD 588
           NLNRGRLE YYW+VSGIQVLNLVYYVVCAWFYTYKPLEEE+   D EG+
Sbjct: 545 NLNRGRLERYYWLVSGIQVLNLVYYVVCAWFYTYKPLEEEK---DDEGE 586

BLAST of MC05g1040 vs. ExPASy TrEMBL
Match: A0A1S4DUU1 (protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo OX=3656 GN=LOC103487163 PE=3 SV=1)

HSP 1 Score: 986 bits (2548), Expect = 0.0
Identity = 483/560 (86.25%), Postives = 524/560 (93.57%), Query Frame = 0

Query: 44  ILGNEVCDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFA 103
           I+GNEVCDRFAS+GFH+NIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFA
Sbjct: 12  IVGNEVCDRFASSGFHSNIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFA 71

Query: 104 GRFWTITVASIIYHLGMATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSL 163
           GRFWTIT+ASIIY LGM TIT+SAILP LHP PCPTQ+NC QASGTQLM  YLALLLTSL
Sbjct: 72  GRFWTITIASIIYELGMVTITISAILPSLHPPPCPTQINCIQASGTQLMFLYLALLLTSL 131

Query: 164 GAGGIRPCVVAFAADQFDMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWG 223
           GAGGIRPCVVAFAADQFDMTKVG+AGR+WNFFNWYYFCMG+ATLTALTVVVYIQDNVGWG
Sbjct: 132 GAGGIRPCVVAFAADQFDMTKVGIAGRTWNFFNWYYFCMGMATLTALTVVVYIQDNVGWG 191

Query: 224 WGFGLPTIAMGLSVVAFVVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLY 283
           WGFGLPTIAM LSVVAFVVGSPLY K+KPSGSPLVRLAQVVVAA KNRKAVLPDD +LLY
Sbjct: 192 WGFGLPTIAMALSVVAFVVGSPLYNKLKPSGSPLVRLAQVVVAAFKNRKAVLPDDSELLY 251

Query: 284 RNHKLDAAIASQGTLVHTNQFKWLDKAAVVTNGERES-PPNLWRVATVHRVEELKSIIRM 343
           RNH+LDAAIA QG LVHT+QFKWLDKAAV+T  +  + PPNLWR+ATVHRVEELKSIIRM
Sbjct: 252 RNHELDAAIAVQGKLVHTDQFKWLDKAAVITTPDSTANPPNLWRLATVHRVEELKSIIRM 311

Query: 344 LPIWAAGILLVTASSHQHSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYER 403
           LPIWAAGILLVTASSHQHSFTIQQARTM+R L+ +FQIPPA+LSIFG LSMLTGLVLY+R
Sbjct: 312 LPIWAAGILLVTASSHQHSFTIQQARTMNRHLTPTFQIPPASLSIFGILSMLTGLVLYDR 371

Query: 404 LLIPFARRFTKNPSGITCLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATI 463
           LL+PFA++ T NP GITCLQRMGVGFA+NILATLVSS VEI+RK+VA  HGLLD PTATI
Sbjct: 372 LLVPFAKKLTHNPLGITCLQRMGVGFAINILATLVSSAVEIKRKKVAANHGLLDNPTATI 431

Query: 464 PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMV 523
           PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRS+ATALYWLAISAGNYIGTLMV
Sbjct: 432 PFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSTATALYWLAISAGNYIGTLMV 491

Query: 524 YVVHKYSGEKHNWLPDRNLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEER-- 583
           Y+VHKY+GE+HNWLPDRNLNRGRLEYYYW+VSGIQV+NLVYYV+CAWFYTYKPLEEE+  
Sbjct: 492 YLVHKYTGEEHNWLPDRNLNRGRLEYYYWLVSGIQVINLVYYVICAWFYTYKPLEEEKTI 551

Query: 584 ---QNSDGEGDQCEDITKTA 597
              ++SD EG++CED +K A
Sbjct: 552 IENKDSDQEGERCEDTSKIA 571

BLAST of MC05g1040 vs. ExPASy TrEMBL
Match: A0A5A7UI29 (Protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006470 PE=3 SV=1)

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 458/532 (86.09%), Postives = 496/532 (93.23%), Query Frame = 0

Query: 72  MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAILPQ 131
           MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTIT+ASIIY LGM TIT+SAILP 
Sbjct: 1   MPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITIASIIYELGMVTITISAILPS 60

Query: 132 LHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRS 191
           LHP PCPTQ+NC QASGTQLM  YLALLLTSLGAGGIRPCVVAFAADQFDMTKVG+AGR+
Sbjct: 61  LHPPPCPTQINCIQASGTQLMFLYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGIAGRT 120

Query: 192 WNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKKIK 251
           WNFFNWYYFCMG+ATLTALTVVVYIQDNVGWGWGFGLPTIAM LSVVAFVVGSPLY K+K
Sbjct: 121 WNFFNWYYFCMGMATLTALTVVVYIQDNVGWGWGFGLPTIAMALSVVAFVVGSPLYNKLK 180

Query: 252 PSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDKAA 311
           PSGSPLVRLAQVVVAA KNRKAVLPDD +LLYRNH+LDAAIA QG LVHT+QFKWLDKAA
Sbjct: 181 PSGSPLVRLAQVVVAAFKNRKAVLPDDSELLYRNHELDAAIAVQGKLVHTDQFKWLDKAA 240

Query: 312 VVTNGERES-PPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTM 371
           V+T  +  + PPNLWR+ATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTM
Sbjct: 241 VITTPDSTANPPNLWRLATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTM 300

Query: 372 DRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGVGFAV 431
           +R L+ +FQIPPA+LSIFG LSMLTGLVLY+RLL+PFA++ T NP GITCLQRMGVGFA+
Sbjct: 301 NRHLTPTFQIPPASLSIFGILSMLTGLVLYDRLLVPFAKKLTHNPLGITCLQRMGVGFAI 360

Query: 432 NILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEF 491
           NILATLVSS VEI+RK+VA  HGLLD PTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEF
Sbjct: 361 NILATLVSSAVEIKRKKVAANHGLLDNPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEF 420

Query: 492 MYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGEKHNWLPDRNLNRGRLEYYY 551
           MYDQSPESLRS+ATALYWLAISAGNYIGTLMVY+VHKY+GE+HNWLPDRNLNRGRLEYYY
Sbjct: 421 MYDQSPESLRSTATALYWLAISAGNYIGTLMVYLVHKYTGEEHNWLPDRNLNRGRLEYYY 480

Query: 552 WVVSGIQVLNLVYYVVCAWFYTYKPLEEER-----QNSDGEGDQCEDITKTA 597
           W+VSGIQV+NLVYYV+CAWFYTYKPLEEE+     ++SD EG++CED +K A
Sbjct: 481 WLVSGIQVINLVYYVICAWFYTYKPLEEEKTIIENKDSDQEGERCEDTSKIA 532

BLAST of MC05g1040 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 669.5 bits (1726), Expect = 2.6e-192
Identity = 327/580 (56.38%), Postives = 430/580 (74.14%), Query Frame = 0

Query: 10  VDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQD 69
           ++E+ K K+ EE +   G  + +R  GG+ TMPFI  NE+C++ A  GFHAN+I+YLT  
Sbjct: 1   MEEQSKNKISEEEKQLHG--RPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQ 60

Query: 70  LNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAIL 129
           L++PL  A+N LTNFA TSS T L+GA IADSFAGRFWTIT ASIIY +GM  +T+SAI+
Sbjct: 61  LHLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAII 120

Query: 130 PQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAG 189
           P L P PC  +  C  A   QL + Y+ALLL +LG+GGIRPCVVAF ADQFD +      
Sbjct: 121 PTLRPPPCKGEEVCVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTT 180

Query: 190 RSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKK 249
           ++WN+FNWYYFCMG A L A+TV+V+IQDNVGWG G G+PT+AM LSV+AFV G  LY+ 
Sbjct: 181 KTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRH 240

Query: 250 IKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDK 309
           + P+GSP  RL QV VAA + RK  +  DP LLY N ++DA I+  G L HT    +LDK
Sbjct: 241 LVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDK 300

Query: 310 AAVVTNGERESP---PNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQ 369
           AA+VT  +   P   PN WR++TVHRVEELKS+IRM PI A+GILL+TA + Q +F++QQ
Sbjct: 301 AAIVTEEDNLKPGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQ 360

Query: 370 ARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGV 429
           A+TM+R L+NSFQIP  ++S+F  ++MLT ++ Y+R+ +  AR+FT    GIT L RMG+
Sbjct: 361 AKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGI 420

Query: 430 GFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVG 489
           GF ++I+ATLV+  VE++RK VA+EHGLLD+P   +P S  WL+PQ+ LHG+AE FMS+G
Sbjct: 421 GFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIG 480

Query: 490 HLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSG--EKHNWLPDRNLNRG 549
           HLEF YDQ+PES+RS+ATAL+W+AIS GNY+ TL+V +VHK+S   +  NWLPD NLNRG
Sbjct: 481 HLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRG 540

Query: 550 RLEYYYWVVSGIQVLNLVYYVVCAWFYTYKPLEEERQNSD 585
           RLEY+YW+++ +Q +NLVYY+ CA  YTYKP++      D
Sbjct: 541 RLEYFYWLITVLQAVNLVYYLWCAKIYTYKPVQVHHSKED 578

BLAST of MC05g1040 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 437.6 bits (1124), Expect = 1.6e-122
Identity = 229/548 (41.79%), Postives = 351/548 (64.05%), Query Frame = 0

Query: 27  GEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQDLNMPLVPASNILTNFAA 86
           G     +K G  K  PFILGNE C+R A  G   N+ITY T +L+   V A++ +  +  
Sbjct: 45  GNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAASDVMIWQG 104

Query: 87  TSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAILPQLHPTPCP--TQLNCT 146
           T   T LIGA+IADS+ GR+WTI   S IY +GMA +T+SA LP L P  C       C+
Sbjct: 105 TCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAGVAAALCS 164

Query: 147 QASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGRSWNFFNWYYFCMGL 206
            A+  Q  VF+  L L +LG GGI+PCV +F ADQFD T      R  +FFNW+YF + +
Sbjct: 165 PATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNWFYFSINI 224

Query: 207 ATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKKIKPSGSPLVRLAQVV 266
            +  + T++V++Q+NVGWG GF +PT+ MG+S+ +F +G+PLY+  KP GSP+ R+ QV+
Sbjct: 225 GSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPITRVCQVL 284

Query: 267 VAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDKAAVVTNGERESP--P 326
           VAA +  K  LP+D   LY   + ++ IA    + HT+ +K+LDKAAV++  E +S    
Sbjct: 285 VAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVISEYESKSGAFS 344

Query: 327 NLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQARTMDRRLSNSFQIPP 386
           N W++ TV +VEE+K++IRM PIWA+GI+     S   +  +QQ R+M+ R+  SF+IPP
Sbjct: 345 NPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMN-RIIRSFEIPP 404

Query: 387 ATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGVGFAVNILATLVSSLVE 446
           A+  +F  L +L  + +Y+R L+PF RRFT  P G+T LQRMG+G  +++L+   +++VE
Sbjct: 405 ASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQRMGIGLFLSVLSIAAAAIVE 464

Query: 447 IRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVGHLEFMYDQSPESLRSS 506
             R ++A +          +  S+FW +PQ+ L GIAEVF  +G +EF YD+SP+++RS 
Sbjct: 465 TVRLQLAQDF---------VAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDESPDAMRSV 524

Query: 507 ATALYWLAISAGNYIGTLMVYVVHKYS--GEKHNWLPDRNLNRGRLEYYYWVVSGIQVLN 566
            +AL  L  + G+Y+ +L++ +V  ++  G K  W+PD +LN+G L+Y++W++  + ++N
Sbjct: 525 CSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPD-DLNKGHLDYFFWLLVSLGLVN 581

Query: 567 L-VYYVVC 568
           + VY ++C
Sbjct: 585 IPVYALIC 581

BLAST of MC05g1040 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 437.2 bits (1123), Expect = 2.1e-122
Identity = 233/568 (41.02%), Postives = 355/568 (62.50%), Query Frame = 0

Query: 11  DEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQDL 70
           D+K+    +  +++ +    K+ K G  K   FILG E C+R A  G   N+I YL + +
Sbjct: 3   DDKDIYTKDGTLDIHKKPANKN-KTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQM 62

Query: 71  NMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAILP 130
           NM  V AS  ++N++ T   T LIGA IAD++ GR+WTI    +IY  GM  +T+SA +P
Sbjct: 63  NMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVP 122

Query: 131 QLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAGR 190
            L PT C  +  C   +G Q  + ++AL L +LG GGI+PCV +F ADQFD T       
Sbjct: 123 GLTPT-CSGE-TCHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKES 182

Query: 191 SWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKKI 250
             +FFNW+YF + +  + A +V+V+IQ NVGWGWG G+PT+AM ++VV F  GS  Y+  
Sbjct: 183 KSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQ 242

Query: 251 KPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDKA 310
           KP GSPL R+ QV+VA+ +  K  +P+D  LLY N   +++I     L HT    + DKA
Sbjct: 243 KPGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKA 302

Query: 311 AVVTNGERE--SPPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQAR 370
           AV T  + +  +  + W++ TV +VEELK++IR+LPIWA GI+  +  S   +  + Q  
Sbjct: 303 AVETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGN 362

Query: 371 TMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGVGF 430
           T+D+ +  +F+IP A+LS+F  LS+L    +Y++L++PFAR++T +  G T LQR+G+G 
Sbjct: 363 TLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGL 422

Query: 431 AVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVGHL 490
            ++I + + + ++E+ R      H L +    TIP ++FW VPQ++L G AEVF  +G L
Sbjct: 423 VISIFSMVSAGILEVARLNYVQTHNLYNE--ETIPMTIFWQVPQYFLVGCAEVFTFIGQL 482

Query: 491 EFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKY--SGEKHNWLPDRNLNRGRL 550
           EF YDQ+P+++RS  +AL   AI+ GNY+ T +V +V K   SG +  W+  +NLN G L
Sbjct: 483 EFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIA-KNLNNGHL 542

Query: 551 EYYYWVVSGIQVLNLVYYVVCAWFYTYK 575
           +Y++W+++G+  LN + Y+  A +YTYK
Sbjct: 543 DYFFWLLAGLSFLNFLVYLWIAKWYTYK 563

BLAST of MC05g1040 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 433.7 bits (1114), Expect = 2.4e-121
Identity = 235/582 (40.38%), Postives = 357/582 (61.34%), Query Frame = 0

Query: 10  VDEKEKKKLEEEMELEE-------------GEKKKDRKLGGVKTMPFILGNEVCDRFAST 69
           ++E+ +  +EE + L+E             G      K G  K  PFILGNE C+R A  
Sbjct: 4   IEEEARPLIEEGLILQEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYY 63

Query: 70  GFHANIITYLTQDLNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIY 129
           G   N+ITYLT  L+   V A+  +T +  T   T LIGA++AD++ GR+WTI   S IY
Sbjct: 64  GIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIY 123

Query: 130 HLGMATITVSAILPQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFA 189
            +GM+ +T+SA +P L P  C     C  A+  Q  +F+  L L +LG GGI+PCV +F 
Sbjct: 124 FIGMSALTLSASVPALKPAECIGDF-CPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFG 183

Query: 190 ADQFDMTKVGVAGRSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLS 249
           ADQFD T      R  +FFNW+YF + +  L + +++V+IQ+N GWG GFG+PT+ MGL+
Sbjct: 184 ADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLA 243

Query: 250 VVAFVVGSPLYKKIKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQG 309
           + +F  G+PLY+  KP GSP+ R++QVVVA+ +     +P+D  LLY     ++AIA   
Sbjct: 244 IASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSR 303

Query: 310 TLVHTNQFKWLDKAAVVTNGERESP--PNLWRVATVHRVEELKSIIRMLPIWAAGILLVT 369
            + HT+  ++LDKAAV++  E +S    N WR+ TV +VEELK +IRM PIWA+GI+   
Sbjct: 304 KIEHTDDCQYLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSA 363

Query: 370 ASSHQHSFTIQQARTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKN 429
             +   +  +QQ R M+ ++  SFQ+PPA L  F   S++  + LY+R ++P AR+FT  
Sbjct: 364 VYAQMSTMFVQQGRAMNCKI-GSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGV 423

Query: 430 PSGITCLQRMGVGFAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFW 489
             G T +QRMG+G  V++L    +++VEI R  +A + GL++   A +P SV W +PQ++
Sbjct: 424 DKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVE-SGAPVPISVLWQIPQYF 483

Query: 490 LHGIAEVFMSVGHLEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYS--GEK 549
           + G AEVF  +G LEF YDQSP+++RS  +AL  L  + GNY+ +L++ +V  ++    +
Sbjct: 484 ILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQ 543

Query: 550 HNWLPDRNLNRGRLEYYYWVVSGIQVLNLVYYVVCAWFYTYK 575
             W+ D NLN G L+Y++W+++G+ ++N+  Y   A  Y  K
Sbjct: 544 EGWISD-NLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of MC05g1040 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 430.6 bits (1106), Expect = 2.0e-120
Identity = 231/569 (40.60%), Postives = 347/569 (60.98%), Query Frame = 0

Query: 10  VDEKEKKKLEEEMELEEGEKKKDRKLGGVKTMPFILGNEVCDRFASTGFHANIITYLTQD 69
           ++EK+    +  +++ +    K+ K G  K   FILGNE C+R A  G   N++ YL   
Sbjct: 1   MEEKDVYTQDGTVDIHKNPANKE-KTGNWKACRFILGNECCERLAYYGMGTNLVNYLESR 60

Query: 70  LNMPLVPASNILTNFAATSSFTSLIGALIADSFAGRFWTITVASIIYHLGMATITVSAIL 129
           LN     A+N +TN++ T   T LIGA IAD++ GR+WTI     IY  GM  +T+SA +
Sbjct: 61  LNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASV 120

Query: 130 PQLHPTPCPTQLNCTQASGTQLMVFYLALLLTSLGAGGIRPCVVAFAADQFDMTKVGVAG 189
           P L P  C    +    + +Q  VF++AL + +LG GGI+PCV +F ADQFD        
Sbjct: 121 PGLKPGNC--NADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKI 180

Query: 190 RSWNFFNWYYFCMGLATLTALTVVVYIQDNVGWGWGFGLPTIAMGLSVVAFVVGSPLYKK 249
           +  +FFNW+YF + +  L A TV+V+IQ NVGWGWGFG+PT+AM ++V  F  GS  Y+ 
Sbjct: 181 KKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRL 240

Query: 250 IKPSGSPLVRLAQVVVAAVKNRKAVLPDDPKLLYRNHKLDAAIASQGTLVHTNQFKWLDK 309
            +P GSPL R+ QV+VAA +     +P+D  LL+     ++ I     LVHT+  K+ DK
Sbjct: 241 QRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDK 300

Query: 310 AAVVTNGE--RESPPNLWRVATVHRVEELKSIIRMLPIWAAGILLVTASSHQHSFTIQQA 369
           AAV +  +  ++   N WR+ +V +VEELKSII +LP+WA GI+  T  S   +  + Q 
Sbjct: 301 AAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQG 360

Query: 370 RTMDRRLSNSFQIPPATLSIFGNLSMLTGLVLYERLLIPFARRFTKNPSGITCLQRMGVG 429
            TMD+ +  +F+IP A+LS+F  +S+L    +Y++ +IP AR+FT+N  G T LQRMG+G
Sbjct: 361 NTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIG 420

Query: 430 FAVNILATLVSSLVEIRRKRVAMEHGLLDRPTATIPFSVFWLVPQFWLHGIAEVFMSVGH 489
             V+I A + + ++E+ R      H   D+    I  S+FW +PQ+ L G AEVF  +G 
Sbjct: 421 LVVSIFAMITAGVLEVVRLDYVKTHNAYDQ--KQIHMSIFWQIPQYLLIGCAEVFTFIGQ 480

Query: 490 LEFMYDQSPESLRSSATALYWLAISAGNYIGTLMVYVVHKYSGE--KHNWLPDRNLNRGR 549
           LEF YDQ+P+++RS  +AL    ++ GNY+ T++V VV K + +  K  W+PD NLNRG 
Sbjct: 481 LEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGH 540

Query: 550 LEYYYWVVSGIQVLNLVYYVVCAWFYTYK 575
           L+Y++++++ +  LN + Y+  +  Y YK
Sbjct: 541 LDYFFYLLATLSFLNFLVYLWISKRYKYK 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX203.6e-19156.38Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Q93Z202.3e-12141.79Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Q9LFB83.0e-12141.02Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
P460323.3e-12040.38Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q9M3902.8e-11940.60Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
NP_001267552.10.084.84probable nitrite transporter At1g68570-like [Cucumis sativus] >KGN59416.1 hypoth... [more]
XP_038903971.10.084.74protein NRT1/ PTR FAMILY 3.1-like isoform X1 [Benincasa hispida][more]
KAG6596175.10.085.01Protein NRT1/ PTR FAMILY 3.1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7027713.10.085.01Protein NRT1/ PTR FAMILY 3.1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022971207.10.085.12protein NRT1/ PTR FAMILY 3.1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q964000.084.84Nitrite transporter OS=Cucumis sativus OX=3659 GN=NiTR1 PE=2 SV=2[more]
A0A6J1I1C50.085.12protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita maxima OX=3661 GN=LOC111469999 PE... [more]
A0A6J1FLW30.086.42protein NRT1/ PTR FAMILY 3.1-like OS=Cucurbita moschata OX=3662 GN=LOC111446603 ... [more]
A0A1S4DUU10.086.25protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo OX=3656 GN=LOC103487163 PE=3 S... [more]
A0A5A7UI290.086.09Protein NRT1/ PTR FAMILY 3.1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G68570.12.6e-19256.38Major facilitator superfamily protein [more]
AT1G62200.11.6e-12241.79Major facilitator superfamily protein [more]
AT5G01180.12.1e-12241.02peptide transporter 5 [more]
AT2G02040.12.4e-12140.38peptide transporter 2 [more]
AT3G54140.12.0e-12040.60peptide transporter 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 8..35
NoneNo IPR availablePANTHERPTHR11654:SF326NITRATE TRANSPORTER 1coord: 26..582
NoneNo IPR availableCDDcd17415MFS_NPF3coord: 38..570
e-value: 0.0
score: 751.202
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 9..579
e-value: 2.4E-182
score: 608.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 53..579
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 106..536
e-value: 2.9E-92
score: 309.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 26..582

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g1040.1MC05g1040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity