MC05g0735 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC05g0735
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description(1->3)-beta-glucan endohydrolase
LocationMC05: 5855899 .. 5860411 (-)
RNA-Seq ExpressionMC05g0735
SyntenyMC05g0735
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCATGGCAACTTTTGCTCTCTATCTCATTCTTCTCCAATCTCTTCTCATTCTCTCTGGTAAGTGTGTGCCTTCTTTCTTTTGCTTTAATTGTATTGCTCCCCATTCCCCACTTTGATATTTTGATTTAGTCTCTAACACAGATACTCTAATTAAACTTTTCCAAGTTTTGATTGTGATAATAAAACATTGAAAATTTCATCGATTGGAATAGACGGATCCGTTTGGTATCTTATTCTCGTTTTCAAATTTTATTAAGAAACTTAACCATTTTGTAACTAAATATGTTTATTGTATCTTCTTTTATTTTGAAACATTATCAAAATTGGATAGAAATCTTGGAAACAATTAAAACAAGTTTCTTGTTTCTCTCCAATATGTTACAATACCTAGATATAAATATTTATTTGATCATTCTAGAATTCATGGGTGTTAGGGCTATACAATTTTATTTTTCTTTATTAAAAATGAAGAAATAGCAATAAAAAACGAGAACATTACCAAACGAACCTGACGTGTTCCCTTATAAAATCGAACGTTTGATAGGACGATAAATAGTTAGCTTTTACATAGGATAGATTTACTAATATCTTAACAGTTGGCCATCAGCTGTGTTTTCTAAAGTAATTTATGACAATAACGACTTAATTAAAAACACTAAATACATAAAAGACAAAATCAATATTTTGGTGGAATGGATTGGATATCTAGCTTGAGAGAAATAACCGTCTTTTCTCATATAAATATAGACCCACAGGTACTCTATGGGATGATATTGAGCTGACTGCTTCTTCTTAGTCACCAAATCCACATTCTTTTGTAATTATGATATCTCCACCATCTTATTAAACTGAAGCACTTTTACTTAGCTACCCTTTATTTTATGTTCCTCCCATACATTTTGAATGAAATGGAACTGTATTTCCTCTTAAAGTAAAAAAAAAAAGACAAAATCAAAATTTTTAAGAGAGATACATAAACGAAATTTAGCAATAACAGCTTGTGAGTAAAGCAAAAACCTAAAGCAGAACTCGGTAGGTTAAGACATCTCTACTTTTTCTACGAGTTCGTATTCAAAGTTCCATATCTAAGATTCCAAAAAAGAAGCAAATAAGGATAATAAATTTAGTCCTAATTTAGCATATTTTTATACTTTTTTGTGTCTAAAGTTTTAGAGTTATACAGACGCAGAGATTTCAAGCAAGATTGGAATAAACTATGGGCAATTGGGGAGCAACTTGCCATCTCCAAATCAGGCCGTGGAGATGATAAAATCCATGAAAGCCGGCCGAGTGAAGCTGTACGACGCCAACCCACAATTCTTGAAGCTCTTATCAGGAACAAAGCTTCAAGTTTCCATCATGATACCCAACGACCAAATCTCAGACATTGCCGCCAACCAGACTAGGGCCGACGAATGGATCCTCGACAATGTCGTCGCTTTTTACCCCGATACCATGATTCGGTTCATTCTGGTAGGAAATGAAGTTTTGAGCTACGATGACCGTCGAGTTTGGCACCAGCTTGTGCCCGCGATGAGGAGCATTTGGCGTTCGCTTAAGGCCCGCAATTTGCAGATCATTAAAGTTGGGACTCCGGTGGCTATGGACGTCTTGGAGTCTGCATTCCCACCCTCAAGAGGGACGTTTAGATCGGATATACGACGAAGCGTGGTGGCTCCCATGCTCGAATTCTTGAACGAGACCAGGTGGGAGTTAAGTTGATAGGTTAGAGTAAATTTGAAATTTTTGTTTCATTATTTGGAGTTTAAAAGAAAAAAACCATTTTGCTCTCAAATTTTTAAAATATTAACTTTTATCACCTAATTAATATTTATTTGTTTAATATTGGTTAAGGTAGTTGCAATACATACTTTACTAGCTTGACATAAATTTACTCCAAGTTAGCGGAATATAGACGAGAATAACTAAAATGATTAACTAAAAATATACATTTTAAAATTTTAAAAATTTAACAAGAAATTTTTTTTCCTTTTGTAATTTTTAACACTTCATCCCCTTTCTTAACTAAAAAATTTAAGTAATGGGTTAGAATACATTTAATCTTTTATTTATTTTCTTAACAGGTCTTATTTCTTTGTCGATGCGTATCCTTATTTTTCTTGGTCGGCAAATCCCAGGGATATCAACTTAGATTTTGCTCTTTTCGAGGCAAATTTTGAACAGATCGACGGTGGGACTGGATTGGCATATACTAATCTTTTGGATGAAATGTTGGACTCTCTTATCTTTGCCATGGCCAAACTTGGCTATCCGGACATTCGTTTGGTGATCTCAGAGACTGGATGGCCGACTGCTGGCGATATCGAACAACCAGGTCCTAAACCACTCACTTAACATGAAATGTAAATCTAAGTTAATAGGTTAACAATAAATATATATATATATATATCATTTATTCATATTCTTGACACTAAGACATTAAAGAACTTTGATGTGCATATGTCAAATTAAATCGTTATTGAACCTTAGAATGAATTTAAGTGGATAGGTTATAAAATATTTAATCTTTTATTCATATTCACAATCCTAGGCGCCAATATCCTCAACGCGGCAACGTACAACCGAAACCTAGTGAAGAAGATGACAGCAAAACCTACGGTAGGGACGCCGGCGAGACCAGGGGTGGTGATACCGACGTTCATATTCGCGCTCTTTGACGAGAACCAAAAGCTAGGGCCGGGAACGGAGCGGCATTGGGGGTTACTAAGCTCTGATGGGAGCCCGAACTACGAGATCGACTTGACCGGAAAGAAGTCGTCGGTGGAGTACAATCCGATGCCGGTGGTGGAGAACAATGTGCCGTTCAAGGGGACGCTGTGGTGCGTGGCGGCGAGAGGGGTGAACTTGATGGAGCTCGGGGCGGCGATCACGACGGTGTGCGGCGGAGGAAACGGGACGTGTGAGGCGCTGTCGCCGGGAAAAGAGTGCTATGAGCCGGTGTCGGTGTATTGGCATGCTAGCTATGCTTTTAGCTCTTTTTGGTCAAAGTTTAGGAGCCAAGGGGCGAGCTGCTATTTCAATGGACTTGCTGAACAGACAACCATTGATCCTAGTGAGTTAATCTCTCTCTCTATTTATGTGCTCCTTAAACTCTATTTAATTTCTATAATTGTCCATACACTTAAAAATTAGTACCATAAATTTTTGAAGGTTTTTCTTCTACTACTTCAAATTTTGTAAATTAGTTTTAGATTTCGTTCTTTTACCATTAAAATCTTTAAGGCGTGCGAAGTAATGACCTGATTCATCTTTTATCGTTTGATATTTCCGATATTTTTAAAGTCTAATCTTTTGTGTTATTTTCCAATTTTGTTTTATGAGTGAGGCAATAGTGTGCACGATACATGGTTTTTATATCATGAACTACTAATTAAGAGATTCATGTTCATGGTAATTAATAATAAAATAAAATATATCATTTTTTTTGGTTAGTTACTAACTTAAACTATAACAGAAAATGACTAATTTAGTACTTAAAAAGACATTAAATATATAATTTCTAACATTTTTAAGACCAAGTTAAGATACATGCATGGATAAAATTTGAAGACCAATACTATATTTTAGTCTCTTTTTTTCAAAGGAAAATTTAATCTATTTTTAGGCACCTCTCCCTTCCTTTTTGGTTGTTATTGTCAAGTTAAATTATATTTCTAATTCATGTTTTTTTTTTAATCTTAATTATTTCAATTTTAAGATATTTAGTTAGCTAGTGTGTGTATATATATATATATATATTTTGCACGGATAACTAAATATTCCTATCATTCATTGTTAATAACAAAAATCTATTATGAAAAGTTATATGTCGATGCACTAATGTAAAACGAGTTATAAAAATCCAATGAGTATAATTGGTTCGATTAATGGAATTGTTGACATAGTAAGCTCATTAGTTAAATTATGATCGAAGAACTAAATTGGTATGATGTTACATACAATCAAATAGACTAAACCCATCATTAAATGTATTTTACTTATATCTAGTATTAATTGTAGAAATTAAATAGTAGCAAAAATATTCGAAATATGTTATGCCATATAATTTATCATCATACCAATTTCAACGCTTAGAAAGAGACTTTGAAAAATGTAAAACTTTTTAATTAATAGGGACTAAATTGAACCGATAGTATTTTTATTATTATTTTAATACATGTTCATTTTTCCTTTTATTTGCTTTAATATTTTCCCCCATGATTTTCTTGGCAGGTAATGGATCATGCAAGTTCCCAAGTGTGACTTTCTAAAGAAGACTTCATAGAATTCTTTTAACTTTAAAAGAACTTCGTAGGATTTTTCTTTTTCTTTCCTTTTTTTCCCAAAGGGAGAAGAAGTTTATAAGGCCGATTTACGATTAGGAGAAATATGTAATGCAAAATTTTATCTTTTTCAGAAATTTGTATCGTTTTATTTACCTACAATAAATAAAGTGGAGAATAATAATAATAATAAAAAAAATCACGTGGGTGGCACGTGTCTTATAATTTTC

mRNA sequence

CCATGGCAACTTTTGCTCTCTATCTCATTCTTCTCCAATCTCTTCTCATTCTCTCTGACGCAGAGATTTCAAGCAAGATTGGAATAAACTATGGGCAATTGGGGAGCAACTTGCCATCTCCAAATCAGGCCGTGGAGATGATAAAATCCATGAAAGCCGGCCGAGTGAAGCTGTACGACGCCAACCCACAATTCTTGAAGCTCTTATCAGGAACAAAGCTTCAAGTTTCCATCATGATACCCAACGACCAAATCTCAGACATTGCCGCCAACCAGACTAGGGCCGACGAATGGATCCTCGACAATGTCGTCGCTTTTTACCCCGATACCATGATTCGGTTCATTCTGGTAGGAAATGAAGTTTTGAGCTACGATGACCGTCGAGTTTGGCACCAGCTTGTGCCCGCGATGAGGAGCATTTGGCGTTCGCTTAAGGCCCGCAATTTGCAGATCATTAAAGTTGGGACTCCGGTGGCTATGGACGTCTTGGAGTCTGCATTCCCACCCTCAAGAGGGACGTTTAGATCGGATATACGACGAAGCGTGGTGGCTCCCATGCTCGAATTCTTGAACGAGACCAGGTCTTATTTCTTTGTCGATGCGTATCCTTATTTTTCTTGGTCGGCAAATCCCAGGGATATCAACTTAGATTTTGCTCTTTTCGAGGCAAATTTTGAACAGATCGACGGTGGGACTGGATTGGCATATACTAATCTTTTGGATGAAATGTTGGACTCTCTTATCTTTGCCATGGCCAAACTTGGCTATCCGGACATTCGTTTGGTGATCTCAGAGACTGGATGGCCGACTGCTGGCGATATCGAACAACCAGGCGCCAATATCCTCAACGCGGCAACGTACAACCGAAACCTAGTGAAGAAGATGACAGCAAAACCTACGGTAGGGACGCCGGCGAGACCAGGGGTGGTGATACCGACGTTCATATTCGCGCTCTTTGACGAGAACCAAAAGCTAGGGCCGGGAACGGAGCGGCATTGGGGGTTACTAAGCTCTGATGGGAGCCCGAACTACGAGATCGACTTGACCGGAAAGAAGTCGTCGGTGGAGTACAATCCGATGCCGGTGGTGGAGAACAATGTGCCGTTCAAGGGGACGCTGTGGTGCGTGGCGGCGAGAGGGGTGAACTTGATGGAGCTCGGGGCGGCGATCACGACGGTGTGCGGCGGAGGAAACGGGACGTGTGAGGCGCTGTCGCCGGGAAAAGAGTGCTATGAGCCGGTGTCGGTGTATTGGCATGCTAGCTATGCTTTTAGCTCTTTTTGGTCAAAGTTTAGGAGCCAAGGGGCGAGCTGCTATTTCAATGGACTTGCTGAACAGACAACCATTGATCCTAGTAATGGATCATGCAAGTTCCCAAGTGTGACTTTCTAAAGAAGACTTCATAGAATTCTTTTAACTTTAAAAGAACTTCGTAGGATTTTTCTTTTTCTTTCCTTTTTTTCCCAAAGGGAGAAGAAGTTTATAAGGCCGATTTACGATTAGGAGAAATATGTAATGCAAAATTTTATCTTTTTCAGAAATTTGTATCGTTTTATTTACCTACAATAAATAAAGTGGAGAATAATAATAATAATAAAAAAAATCACGTGGGTGGCACGTGTCTTATAATTTTC

Coding sequence (CDS)

ATGGCAACTTTTGCTCTCTATCTCATTCTTCTCCAATCTCTTCTCATTCTCTCTGACGCAGAGATTTCAAGCAAGATTGGAATAAACTATGGGCAATTGGGGAGCAACTTGCCATCTCCAAATCAGGCCGTGGAGATGATAAAATCCATGAAAGCCGGCCGAGTGAAGCTGTACGACGCCAACCCACAATTCTTGAAGCTCTTATCAGGAACAAAGCTTCAAGTTTCCATCATGATACCCAACGACCAAATCTCAGACATTGCCGCCAACCAGACTAGGGCCGACGAATGGATCCTCGACAATGTCGTCGCTTTTTACCCCGATACCATGATTCGGTTCATTCTGGTAGGAAATGAAGTTTTGAGCTACGATGACCGTCGAGTTTGGCACCAGCTTGTGCCCGCGATGAGGAGCATTTGGCGTTCGCTTAAGGCCCGCAATTTGCAGATCATTAAAGTTGGGACTCCGGTGGCTATGGACGTCTTGGAGTCTGCATTCCCACCCTCAAGAGGGACGTTTAGATCGGATATACGACGAAGCGTGGTGGCTCCCATGCTCGAATTCTTGAACGAGACCAGGTCTTATTTCTTTGTCGATGCGTATCCTTATTTTTCTTGGTCGGCAAATCCCAGGGATATCAACTTAGATTTTGCTCTTTTCGAGGCAAATTTTGAACAGATCGACGGTGGGACTGGATTGGCATATACTAATCTTTTGGATGAAATGTTGGACTCTCTTATCTTTGCCATGGCCAAACTTGGCTATCCGGACATTCGTTTGGTGATCTCAGAGACTGGATGGCCGACTGCTGGCGATATCGAACAACCAGGCGCCAATATCCTCAACGCGGCAACGTACAACCGAAACCTAGTGAAGAAGATGACAGCAAAACCTACGGTAGGGACGCCGGCGAGACCAGGGGTGGTGATACCGACGTTCATATTCGCGCTCTTTGACGAGAACCAAAAGCTAGGGCCGGGAACGGAGCGGCATTGGGGGTTACTAAGCTCTGATGGGAGCCCGAACTACGAGATCGACTTGACCGGAAAGAAGTCGTCGGTGGAGTACAATCCGATGCCGGTGGTGGAGAACAATGTGCCGTTCAAGGGGACGCTGTGGTGCGTGGCGGCGAGAGGGGTGAACTTGATGGAGCTCGGGGCGGCGATCACGACGGTGTGCGGCGGAGGAAACGGGACGTGTGAGGCGCTGTCGCCGGGAAAAGAGTGCTATGAGCCGGTGTCGGTGTATTGGCATGCTAGCTATGCTTTTAGCTCTTTTTGGTCAAAGTTTAGGAGCCAAGGGGCGAGCTGCTATTTCAATGGACTTGCTGAACAGACAACCATTGATCCTAGTAATGGATCATGCAAGTTCCCAAGTGTGACTTTCTAA

Protein sequence

MATFALYLILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF
Homology
BLAST of MC05g0735 vs. ExPASy Swiss-Prot
Match: Q06915 (Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=A6 PE=2 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 7.9e-162
Identity = 261/441 (59.18%), Postives = 334/441 (75.74%), Query Frame = 0

Query: 21  EISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIP 80
           +++SKIGINYG+ G+NLPSP Q++  IKS+KAG VKLYDA+P+ L LLS T L V+I +P
Sbjct: 37  DLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVP 96

Query: 81  NDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIW 140
           N QI+ +++NQT ADEW+  N++ +YP T IRF+LVGNE+LSY+   V   LVPAMR I 
Sbjct: 97  NHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIV 156

Query: 141 RSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDA 200
            SL+   +  IKVGTP+AMD L S+FPPS GTFR +I   V+ P+L+FLN T SYFF++ 
Sbjct: 157 NSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNV 216

Query: 201 YPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRL 260
           +PYF WS NP + +LDFALF+ +    D  TGL Y NLLD+MLDS++FAM KLGYP +RL
Sbjct: 217 HPYFRWSRNPMNTSLDFALFQGHSTYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMRL 276

Query: 261 VISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDE 320
            ISETGWP  GDI++ GANILNAATYNRNL+KKM+A P +GTP+RPG+ IPTF+F+LF+E
Sbjct: 277 AISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFNE 336

Query: 321 NQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGV 380
           NQK G GT+RHWG+L  DGSP Y++D TG+     +NP+P   NNVP+KG +WCV   G 
Sbjct: 337 NQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEGA 396

Query: 381 NLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFN 440
           N  EL   +   C   N TC AL+PG+ECYEPVS+YWHASYA +S+W++FR+Q   C+FN
Sbjct: 397 NETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFFN 456

Query: 441 GLAEQTTIDPSNGSCKFPSVT 462
           GLA +TT +P N  CKFPSVT
Sbjct: 457 GLAHETTTNPGNDRCKFPSVT 477

BLAST of MC05g0735 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.5e-83
Identity = 159/446 (35.65%), Postives = 261/446 (58.52%), Query Frame = 0

Query: 14  LLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDANPQFLKLLSGTKL 73
           LL+L D     K+G+ YG+   +LP+P++ V++I+      V++YD N Q LK    T +
Sbjct: 14  LLLLLDCCYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSI 73

Query: 74  QVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEVLSYDDRRVWHQ-- 133
           ++ I +PN  ++  + +Q+  D W+ ++V+ +YP T I +I VG E  S DD  +     
Sbjct: 74  ELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAE--STDDPHINASSF 133

Query: 134 LVPAMRSIWRSLKARNL-QIIKVGTPVAMDVLESAFPPSRGTFRSDIRRSVVAPMLEFLN 193
           +VPAM+++  +L+   L + IKV T +++ +L  +FPPS G F S      + PMLEFL 
Sbjct: 134 VVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSY-AYFLRPMLEFLA 193

Query: 194 ETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTNLLDEMLDSLIFAM 253
           E +S F +D YPY+++  +P +++LD+ LFE++ E ID  TGL Y N+ D  +D+L +A+
Sbjct: 194 ENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYAL 253

Query: 254 AKLGYPDIRLVISETGWPTAGD-IEQPGANILNAATYNRNLVKKMTAKPTVGTPARPGVV 313
             L +  I+++++ETGWPT G   E+  A+  NA TYN N+++ +      GTPA+PG  
Sbjct: 254 TALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQ--GTPAKPGEA 313

Query: 314 IPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNPMPVVENNVPFK 373
           +  +IF+LF+EN+K G  +ER+WGL   D +  Y++D TGK +    N       N    
Sbjct: 314 MNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSS---GTNSSGS 373

Query: 374 GTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYWHASYAFSSFWSK 433
              WC+A+   +  +L  A+   CG GN  C A+ P + C++P ++  HAS+ F+S++ +
Sbjct: 374 SNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQ 433

Query: 434 FRSQGASCYFNGLAEQTTIDPSNGSC 456
            R+   +C F G   +   DPS   C
Sbjct: 434 NRATDVACSFGGAGVKVNKDPSYDKC 451

BLAST of MC05g0735 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 311.2 bits (796), Expect = 1.9e-83
Identity = 165/434 (38.02%), Postives = 262/434 (60.37%), Query Frame = 0

Query: 26  IGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQIS 85
           IG+NYGQ+  NLP P++ V++++S    +V+LY A+P  +K L+GT + + I   N  + 
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVP 85

Query: 86  DIAANQTRADEWILDNVVAFYPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIWRSLKA 145
            +A++   A +WI  NV+ FYP + I  I VGNE+L  +D  + +QL+PAM+++ ++L+A
Sbjct: 86  SLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEA 145

Query: 146 RNL-QIIKVGTPVAMDVLESAFPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYF 205
            +L   IKV T  +M VL S+ PPS G+F +  +  +   +L+FL++T S F ++ YP+F
Sbjct: 146 VSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKG-ILQFLSDTGSPFAINPYPFF 205

Query: 206 SWSANPRDINLDFALFEANFEQIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISE 265
           ++ ++PR   L F LFE N  ++D  TG+ YTN+ D  +D++  A+  +G+  + +V++E
Sbjct: 206 AYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAE 265

Query: 266 TGWPTAGDIEQPGANILNAATYNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKL 325
           TGW + GD  + GA++ NA  YN NL+  +  +  VGTP  PG  + T+IFAL+DEN K 
Sbjct: 266 TGWASRGDANEVGASVDNAKAYNGNLIAHL--RSMVGTPLMPGKPVDTYIFALYDENLKP 325

Query: 326 GPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLME 385
           GP +ER +GL  +D S  Y++ L  K SS    P   V ++       WCV  +G    E
Sbjct: 326 GPSSERAFGLFKTDLSMVYDVGL-AKSSSSSQTPSGKVTSSG------WCVPKKGATNEE 385

Query: 386 LGAAITTVCGGGNGTCEALSPGKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAE 445
           L A++   CG G   C A+ PG  C+EP +V  HA+YA + ++ K   Q   C F+  A 
Sbjct: 386 LQASLDWACGHGI-DCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTAT 445

Query: 446 QTTIDPSNGSCKFP 459
            T+ +PS  +C +P
Sbjct: 446 VTSQNPSYNNCVYP 448

BLAST of MC05g0735 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.8e-82
Identity = 162/466 (34.76%), Postives = 268/466 (57.51%), Query Frame = 0

Query: 13  SLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDANPQFLKLLSGTK 72
           S+L   +  ++SKIGI YG+   NLPSPN+  E+I+ +    V++YDAN   LK  + T 
Sbjct: 14  SILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTG 73

Query: 73  LQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEVLSYDDRRVWHQL 132
           +++ I +PN  +   A  Q+  D W+ +N++ +YP T I  I VG EV    D      +
Sbjct: 74  IELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNAT-GLV 133

Query: 133 VPAMRSIWRSLKARNL-QIIKVGTPVAMDVLESAFPPSRGTFRSDIRRSVVAPMLEFLNE 192
           +PAMR+I  +LK   L + IK+ +  ++ +L  +FPPS  +F S    + + PMLEFL E
Sbjct: 134 LPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASF-SKKHSAFLKPMLEFLVE 193

Query: 193 TRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTNLLDEMLDSLIFAMA 252
             S F +D YPY+++  +   + L++ALFE++ + +D  TGL Y+N+ D  LD++ FA+ 
Sbjct: 194 NESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALT 253

Query: 253 KLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAKPTVGTPARPGVVIP 312
            + +  ++++++E+GWP+ G  ++  A   NA  YN NL++ +   P  GTPA+PG  I 
Sbjct: 254 AMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDP--GTPAKPGEEID 313

Query: 313 TFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKK------------SSVEYNPM 372
            ++F+LF+EN+K G  +ER+WG+  ++G+  Y +D TG+             +S   +  
Sbjct: 314 VYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSS 373

Query: 373 PVVENN--VPFKG------TLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYE 432
           P++  N  V   G        WC+A+   ++ EL  A+   CG GN  C A+ P + C+E
Sbjct: 374 PIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFE 433

Query: 433 PVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKF 458
           P +V  HASYAF++++ +  +    C FNG + +   DPS G+C +
Sbjct: 434 PDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of MC05g0735 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 304.7 bits (779), Expect = 1.8e-81
Identity = 173/467 (37.04%), Postives = 272/467 (58.24%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDAEIS------SKIGINYGQLGSNLPSPNQAVEMIKSMKAGR 60
           MA+    L+L  SLL+L+ A  S      S IG+N G   S++P P Q V ++K+ +   
Sbjct: 1   MASLLHLLLLSLSLLVLASASPSPPADEGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRH 60

Query: 61  VKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFI 120
           ++LY+A+P  L  L+ T ++V I IPNDQ+  I  + + A  W+  NV+A YP TMI  +
Sbjct: 61  IRLYNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAV 120

Query: 121 LVGNEVLSYDDRRVWHQLVPAMRSIWRSLKARNL-QIIKVGTPVAMDVLESAFPPSRGTF 180
            VG+EVL+         LV A++++  +L + NL ++IKV TP++  ++   FPPS+  F
Sbjct: 121 SVGSEVLTSLSNAA-PVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFF 180

Query: 181 RSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFE---ANFEQIDGG 240
              +  +V+ P+L FL  T SY  V+ YPY  +  +   I LD+ALF+    N E +D  
Sbjct: 181 NRSL-NAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKEAVDAN 240

Query: 241 TGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNL 300
           T + Y+N  D M+D+  FAMA L + +I ++++E+GWP+ G+  +P A + NA TYN NL
Sbjct: 241 TLVRYSNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANTYNSNL 300

Query: 301 VKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGK 360
           ++ +  K   GTP RPG+ + T+I+ L++E+ K G  +E++WGL +++G P Y + LT  
Sbjct: 301 IRHVLNK--TGTPKRPGIAVSTYIYELYNEDTKAGL-SEKNWGLFNANGEPVYVLRLTNS 360

Query: 361 KSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECY 420
            S        V+ N+       +C A  G +   L AA+   CG G   C  +  G+ CY
Sbjct: 361 GS--------VLANDT--TNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCY 420

Query: 421 EPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKF 458
           EP +V  HA+YAF +++ +  +   +C FNG+A  TT DPS+G+C F
Sbjct: 421 EPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPSHGTCVF 452

BLAST of MC05g0735 vs. NCBI nr
Match: XP_022138211.1 (probable glucan endo-1,3-beta-glucosidase A6 [Momordica charantia])

HSP 1 Score: 853 bits (2205), Expect = 6.11e-312
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 46  MIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAF 105
           MIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAF
Sbjct: 1   MIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAF 60

Query: 106 YPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESA 165
           YPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESA
Sbjct: 61  YPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESA 120

Query: 166 FPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFE 225
           FPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFE
Sbjct: 121 FPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFE 180

Query: 226 QIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAAT 285
           QIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAAT
Sbjct: 181 QIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAAT 240

Query: 286 YNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEI 345
           YNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEI
Sbjct: 241 YNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEI 300

Query: 346 DLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSP 405
           DLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSP
Sbjct: 301 DLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSP 360

Query: 406 GKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           GKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF
Sbjct: 361 GKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 417

BLAST of MC05g0735 vs. NCBI nr
Match: XP_038905649.1 (probable glucan endo-1,3-beta-glucosidase A6 isoform X2 [Benincasa hispida])

HSP 1 Score: 808 bits (2088), Expect = 2.73e-293
Identity = 396/467 (84.80%), Postives = 433/467 (92.72%), Query Frame = 0

Query: 1   MATFAL-YLILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYD 60
           MATF L YLIL  SLL +SDAEISSKIGINYGQLG+NLP PN A+EM+ SMK GRVKLYD
Sbjct: 1   MATFVLSYLILFFSLLFISDAEISSKIGINYGQLGNNLPPPNLAIEMMNSMKVGRVKLYD 60

Query: 61  ANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNE 120
           AN + L LLS TKLQVSIMIPN++ISDIA NQTRAD+WIL+NVV FYP+TMIRFILVGNE
Sbjct: 61  ANHEILSLLSRTKLQVSIMIPNNEISDIANNQTRADQWILNNVVPFYPNTMIRFILVGNE 120

Query: 121 VLSYD---DRRVWHQLVPAMRSIWRSLKARNLQ-IIKVGTPVAMDVLESAFPPSRGTFRS 180
           VLSYD   DR+VW+ LVPAMR IW SLKA NLQ II+VGTPVAMDVLE+AFPPSRGTFRS
Sbjct: 121 VLSYDSDMDRQVWNDLVPAMRRIWSSLKANNLQQIIRVGTPVAMDVLETAFPPSRGTFRS 180

Query: 181 DIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAY 240
           DIRR+VVAPML+FLNETRS+FF D YPYFSWSANP +I+LDFALF  NFEQIDGG+GL Y
Sbjct: 181 DIRRTVVAPMLDFLNETRSFFFADVYPYFSWSANPMNIDLDFALFNGNFEQIDGGSGLVY 240

Query: 241 TNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMT 300
           TNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPT GDIE+PGAN+LNAATYNRNLVK++T
Sbjct: 241 TNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTTGDIEEPGANLLNAATYNRNLVKRIT 300

Query: 301 AKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVE 360
           AKPT+GTPARPGVVIPTFIFALF+ENQK GPGTERHWGLLSSDGSPNY+IDLTGKKSSVE
Sbjct: 301 AKPTIGTPARPGVVIPTFIFALFNENQKSGPGTERHWGLLSSDGSPNYQIDLTGKKSSVE 360

Query: 361 YNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSV 420
           Y+P+PVVENNVPF+G LWCVAARGV+LMELGAA+  VCGGG+GTCEALSPG+ECYEPVSV
Sbjct: 361 YDPLPVVENNVPFRGRLWCVAARGVDLMELGAAVDDVCGGGDGTCEALSPGRECYEPVSV 420

Query: 421 YWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           YWHASYAFSS+WSKFRSQGA+CYFNGLAEQTTIDPSNGSC+FPSVTF
Sbjct: 421 YWHASYAFSSYWSKFRSQGANCYFNGLAEQTTIDPSNGSCRFPSVTF 467

BLAST of MC05g0735 vs. NCBI nr
Match: XP_022944377.1 (probable glucan endo-1,3-beta-glucosidase A6 isoform X2 [Cucurbita moschata])

HSP 1 Score: 789 bits (2038), Expect = 1.04e-285
Identity = 382/465 (82.15%), Postives = 425/465 (91.40%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDA 60
           M TF+L  +LL SL+ +S AEISSKIGINYGQLGSNLP P+ A+EMIKSMKAGRVKLYDA
Sbjct: 1   MPTFSLNFVLLFSLICISGAEISSKIGINYGQLGSNLPPPSLAIEMIKSMKAGRVKLYDA 60

Query: 61  NPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEV 120
           N + L+LLSGT LQVSIMIPN++ISDIA NQTR+DEWIL+NVV FYPDTMIRFILVGNEV
Sbjct: 61  NHEILRLLSGTNLQVSIMIPNNEISDIANNQTRSDEWILNNVVPFYPDTMIRFILVGNEV 120

Query: 121 LSYD---DRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDI 180
           LSY    DR VW  LVPAMR +W+SLKA+NLQII+VGTPVAMDVLE++FPPS GTFRSDI
Sbjct: 121 LSYASDRDRHVWTDLVPAMRRVWQSLKAQNLQIIRVGTPVAMDVLETSFPPSIGTFRSDI 180

Query: 181 RRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTN 240
           RR+VVAP+LEFLNETRS+FFVDAYPYF WSANP DINLDFALF  NFEQID G+GL YTN
Sbjct: 181 RRTVVAPLLEFLNETRSFFFVDAYPYFPWSANPMDINLDFALFNGNFEQIDRGSGLVYTN 240

Query: 241 LLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAK 300
           LLDEMLDSLIFAMAKLGYPDIRLVISETGWPT GDIEQPGAN+LNAA YNRNLVK++ AK
Sbjct: 241 LLDEMLDSLIFAMAKLGYPDIRLVISETGWPTTGDIEQPGANLLNAAMYNRNLVKRIMAK 300

Query: 301 PTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYN 360
           PT+GTPARPGVVIPTFIFALFDENQK GPGTERHWGLLSSDGSPNY+IDLTG+KSSVEY+
Sbjct: 301 PTIGTPARPGVVIPTFIFALFDENQKSGPGTERHWGLLSSDGSPNYDIDLTGRKSSVEYD 360

Query: 361 PMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYW 420
           P+PVVENNVPF+G LWCVAARGV+LMELGAA+T VC GG+G CE L+PG+ECYEPVSV+W
Sbjct: 361 PIPVVENNVPFRGRLWCVAARGVDLMELGAAVTDVCNGGDGICEELTPGRECYEPVSVHW 420

Query: 421 HASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           HASYAFSS+W++FRSQG SCYFNGLAE+TTIDPS+G C+FPSV F
Sbjct: 421 HASYAFSSYWARFRSQGGSCYFNGLAEETTIDPSSGLCRFPSVIF 465

BLAST of MC05g0735 vs. NCBI nr
Match: XP_022972257.1 (probable glucan endo-1,3-beta-glucosidase A6 isoform X1 [Cucurbita maxima])

HSP 1 Score: 786 bits (2029), Expect = 2.45e-284
Identity = 380/465 (81.72%), Postives = 422/465 (90.75%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDA 60
           M TF+L  +LL SL+ +S AEISSKIGINYGQLGSNLP P+ A+EMIKSMKAGRVKLYDA
Sbjct: 1   MPTFSLNFVLLLSLIFISGAEISSKIGINYGQLGSNLPPPSLAIEMIKSMKAGRVKLYDA 60

Query: 61  NPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEV 120
           N + L+LLSGT LQVSIMIPN++ISDIA NQTR+DEWIL+NVV FYPDTMIRFILVGNEV
Sbjct: 61  NHEILRLLSGTNLQVSIMIPNNEISDIANNQTRSDEWILNNVVPFYPDTMIRFILVGNEV 120

Query: 121 LSYD---DRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDI 180
           LSY    DR VW  LVPAMR +W+SLKA+NLQII+VGTPVAMDVLE++FPPS GTFRSDI
Sbjct: 121 LSYASDRDRHVWTDLVPAMRRVWQSLKAQNLQIIRVGTPVAMDVLETSFPPSTGTFRSDI 180

Query: 181 RRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTN 240
           +R+VVAPMLEFLNETRS+FFVDAYPYF WSANP DINLDFALF  NFEQIDGG+GL YTN
Sbjct: 181 QRTVVAPMLEFLNETRSFFFVDAYPYFPWSANPMDINLDFALFNGNFEQIDGGSGLVYTN 240

Query: 241 LLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAK 300
           LLDEMLDSLIFAM KLGY DIRLVISETGWPT GDIEQPGAN+ NAA YNRNLVK++ AK
Sbjct: 241 LLDEMLDSLIFAMTKLGYSDIRLVISETGWPTTGDIEQPGANLPNAAMYNRNLVKRIVAK 300

Query: 301 PTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYN 360
           PT+GTPARPGVVIPTFIFALFDENQK GPGTERHWGLLSSDGSPNY+IDLTG+KSSVEY+
Sbjct: 301 PTIGTPARPGVVIPTFIFALFDENQKSGPGTERHWGLLSSDGSPNYDIDLTGRKSSVEYD 360

Query: 361 PMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYW 420
           P+PVVENNVPF+G LWCVAARGV+LMELGAA+T VC GG+G CE L PG+ECYEPVSV+W
Sbjct: 361 PIPVVENNVPFRGRLWCVAARGVDLMELGAAVTDVCNGGDGICEELMPGRECYEPVSVHW 420

Query: 421 HASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           HASYAFSS+W++FRSQG SCYFNGLAE+TTIDPS+G C+FPSV F
Sbjct: 421 HASYAFSSYWARFRSQGGSCYFNGLAEETTIDPSSGLCRFPSVIF 465

BLAST of MC05g0735 vs. NCBI nr
Match: XP_023540016.1 (probable glucan endo-1,3-beta-glucosidase A6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 786 bits (2029), Expect = 2.45e-284
Identity = 381/465 (81.94%), Postives = 422/465 (90.75%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDA 60
           M TF+L  +LL SL+ +S AEISSKIGINYGQLGSNLP P+ A+EMIKSMKAGRVKLYDA
Sbjct: 1   MPTFSLNFVLLFSLICISGAEISSKIGINYGQLGSNLPPPSLAIEMIKSMKAGRVKLYDA 60

Query: 61  NPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEV 120
           N + L+LLSGT LQVSIMIPN++ISDIA NQTR+DEWIL+NVV FYPDTMIRFILVGNEV
Sbjct: 61  NHEILRLLSGTNLQVSIMIPNNEISDIANNQTRSDEWILNNVVPFYPDTMIRFILVGNEV 120

Query: 121 LSYD---DRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDI 180
           LSY    DR VW  LVPAMR +W+SLKA+NLQII+VGTPVAMDVLE++FPPS GTFRSDI
Sbjct: 121 LSYASDRDRHVWTDLVPAMRRVWQSLKAQNLQIIRVGTPVAMDVLETSFPPSIGTFRSDI 180

Query: 181 RRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTN 240
           RR+VVAPMLEFLNETRS+FFVDAYPYF WSANP DINLDFALF  NFEQID G+GL YTN
Sbjct: 181 RRTVVAPMLEFLNETRSFFFVDAYPYFPWSANPMDINLDFALFNGNFEQIDRGSGLVYTN 240

Query: 241 LLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAK 300
           LLDEMLDSLIFAM KLGYPDIRLVISETGWPT GDIEQPGAN+LNAA YNRNLVK++ AK
Sbjct: 241 LLDEMLDSLIFAMTKLGYPDIRLVISETGWPTTGDIEQPGANLLNAAMYNRNLVKRIMAK 300

Query: 301 PTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYN 360
           PT+GTPARPGVVIPTFIFALFDENQK GPGTERHWGLLSSDGSPNY+IDLTG KSSVEY+
Sbjct: 301 PTIGTPARPGVVIPTFIFALFDENQKSGPGTERHWGLLSSDGSPNYDIDLTGTKSSVEYD 360

Query: 361 PMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYW 420
           P+PVVENNVPF+G LWCVAARGV+LMELGAA+T VC GG+G CE L+PG+ECYEPVSV+W
Sbjct: 361 PIPVVENNVPFRGRLWCVAARGVDLMELGAAVTDVCNGGDGICEELTPGRECYEPVSVHW 420

Query: 421 HASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           HASYAFSS+W++FRSQG SCYFNGLAE+TT DPS+G C+FPSV F
Sbjct: 421 HASYAFSSYWARFRSQGGSCYFNGLAEETTTDPSSGLCRFPSVIF 465

BLAST of MC05g0735 vs. ExPASy TrEMBL
Match: A0A6J1C925 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111009439 PE=3 SV=1)

HSP 1 Score: 853 bits (2205), Expect = 2.96e-312
Identity = 417/417 (100.00%), Postives = 417/417 (100.00%), Query Frame = 0

Query: 46  MIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAF 105
           MIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAF
Sbjct: 1   MIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAF 60

Query: 106 YPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESA 165
           YPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESA
Sbjct: 61  YPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESA 120

Query: 166 FPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFE 225
           FPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFE
Sbjct: 121 FPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFE 180

Query: 226 QIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAAT 285
           QIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAAT
Sbjct: 181 QIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAAT 240

Query: 286 YNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEI 345
           YNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEI
Sbjct: 241 YNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEI 300

Query: 346 DLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSP 405
           DLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSP
Sbjct: 301 DLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSP 360

Query: 406 GKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           GKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF
Sbjct: 361 GKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 417

BLAST of MC05g0735 vs. ExPASy TrEMBL
Match: A0A6J1FVI9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111448824 PE=3 SV=1)

HSP 1 Score: 789 bits (2038), Expect = 5.06e-286
Identity = 382/465 (82.15%), Postives = 425/465 (91.40%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDA 60
           M TF+L  +LL SL+ +S AEISSKIGINYGQLGSNLP P+ A+EMIKSMKAGRVKLYDA
Sbjct: 1   MPTFSLNFVLLFSLICISGAEISSKIGINYGQLGSNLPPPSLAIEMIKSMKAGRVKLYDA 60

Query: 61  NPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEV 120
           N + L+LLSGT LQVSIMIPN++ISDIA NQTR+DEWIL+NVV FYPDTMIRFILVGNEV
Sbjct: 61  NHEILRLLSGTNLQVSIMIPNNEISDIANNQTRSDEWILNNVVPFYPDTMIRFILVGNEV 120

Query: 121 LSYD---DRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDI 180
           LSY    DR VW  LVPAMR +W+SLKA+NLQII+VGTPVAMDVLE++FPPS GTFRSDI
Sbjct: 121 LSYASDRDRHVWTDLVPAMRRVWQSLKAQNLQIIRVGTPVAMDVLETSFPPSIGTFRSDI 180

Query: 181 RRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTN 240
           RR+VVAP+LEFLNETRS+FFVDAYPYF WSANP DINLDFALF  NFEQID G+GL YTN
Sbjct: 181 RRTVVAPLLEFLNETRSFFFVDAYPYFPWSANPMDINLDFALFNGNFEQIDRGSGLVYTN 240

Query: 241 LLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAK 300
           LLDEMLDSLIFAMAKLGYPDIRLVISETGWPT GDIEQPGAN+LNAA YNRNLVK++ AK
Sbjct: 241 LLDEMLDSLIFAMAKLGYPDIRLVISETGWPTTGDIEQPGANLLNAAMYNRNLVKRIMAK 300

Query: 301 PTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYN 360
           PT+GTPARPGVVIPTFIFALFDENQK GPGTERHWGLLSSDGSPNY+IDLTG+KSSVEY+
Sbjct: 301 PTIGTPARPGVVIPTFIFALFDENQKSGPGTERHWGLLSSDGSPNYDIDLTGRKSSVEYD 360

Query: 361 PMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYW 420
           P+PVVENNVPF+G LWCVAARGV+LMELGAA+T VC GG+G CE L+PG+ECYEPVSV+W
Sbjct: 361 PIPVVENNVPFRGRLWCVAARGVDLMELGAAVTDVCNGGDGICEELTPGRECYEPVSVHW 420

Query: 421 HASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           HASYAFSS+W++FRSQG SCYFNGLAE+TTIDPS+G C+FPSV F
Sbjct: 421 HASYAFSSYWARFRSQGGSCYFNGLAEETTIDPSSGLCRFPSVIF 465

BLAST of MC05g0735 vs. ExPASy TrEMBL
Match: A0A6J1I4C1 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111470842 PE=3 SV=1)

HSP 1 Score: 786 bits (2029), Expect = 1.19e-284
Identity = 380/465 (81.72%), Postives = 422/465 (90.75%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDA 60
           M TF+L  +LL SL+ +S AEISSKIGINYGQLGSNLP P+ A+EMIKSMKAGRVKLYDA
Sbjct: 1   MPTFSLNFVLLLSLIFISGAEISSKIGINYGQLGSNLPPPSLAIEMIKSMKAGRVKLYDA 60

Query: 61  NPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEV 120
           N + L+LLSGT LQVSIMIPN++ISDIA NQTR+DEWIL+NVV FYPDTMIRFILVGNEV
Sbjct: 61  NHEILRLLSGTNLQVSIMIPNNEISDIANNQTRSDEWILNNVVPFYPDTMIRFILVGNEV 120

Query: 121 LSYD---DRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDI 180
           LSY    DR VW  LVPAMR +W+SLKA+NLQII+VGTPVAMDVLE++FPPS GTFRSDI
Sbjct: 121 LSYASDRDRHVWTDLVPAMRRVWQSLKAQNLQIIRVGTPVAMDVLETSFPPSTGTFRSDI 180

Query: 181 RRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTN 240
           +R+VVAPMLEFLNETRS+FFVDAYPYF WSANP DINLDFALF  NFEQIDGG+GL YTN
Sbjct: 181 QRTVVAPMLEFLNETRSFFFVDAYPYFPWSANPMDINLDFALFNGNFEQIDGGSGLVYTN 240

Query: 241 LLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAK 300
           LLDEMLDSLIFAM KLGY DIRLVISETGWPT GDIEQPGAN+ NAA YNRNLVK++ AK
Sbjct: 241 LLDEMLDSLIFAMTKLGYSDIRLVISETGWPTTGDIEQPGANLPNAAMYNRNLVKRIVAK 300

Query: 301 PTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYN 360
           PT+GTPARPGVVIPTFIFALFDENQK GPGTERHWGLLSSDGSPNY+IDLTG+KSSVEY+
Sbjct: 301 PTIGTPARPGVVIPTFIFALFDENQKSGPGTERHWGLLSSDGSPNYDIDLTGRKSSVEYD 360

Query: 361 PMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYW 420
           P+PVVENNVPF+G LWCVAARGV+LMELGAA+T VC GG+G CE L PG+ECYEPVSV+W
Sbjct: 361 PIPVVENNVPFRGRLWCVAARGVDLMELGAAVTDVCNGGDGICEELMPGRECYEPVSVHW 420

Query: 421 HASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           HASYAFSS+W++FRSQG SCYFNGLAE+TTIDPS+G C+FPSV F
Sbjct: 421 HASYAFSSYWARFRSQGGSCYFNGLAEETTIDPSSGLCRFPSVIF 465

BLAST of MC05g0735 vs. ExPASy TrEMBL
Match: A0A1S3AYG2 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103483949 PE=3 SV=1)

HSP 1 Score: 782 bits (2019), Expect = 4.27e-283
Identity = 381/467 (81.58%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 1   MATFALY--LILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLY 60
           MATFAL   L L  SL+++SDAEISSKIG+NYGQLG+NLP PN A+EM+ SMK GRVKLY
Sbjct: 1   MATFALNCSLFLFFSLILISDAEISSKIGVNYGQLGNNLPPPNLAIEMMNSMKVGRVKLY 60

Query: 61  DANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGN 120
           DAN + L  LS TK QVSIMIPN++ISDIA NQTRAD+WI +NV+ FYP TMIRFILVGN
Sbjct: 61  DANHEILGNLSKTKFQVSIMIPNNEISDIANNQTRADQWISNNVLPFYPQTMIRFILVGN 120

Query: 121 EVLSYD---DRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRS 180
           EVLS+D   DR+VW+ LVPAMR IW SLKA NLQ IKVGTPVAMD+LE+ FPPSRGTFRS
Sbjct: 121 EVLSFDSDKDRQVWNDLVPAMRRIWSSLKANNLQTIKVGTPVAMDILETTFPPSRGTFRS 180

Query: 181 DIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAY 240
           DI+++VVAPML FLNETRS+FF + YPYFSW+ANP +INLDFALF  NFEQIDGG+GL Y
Sbjct: 181 DIQQTVVAPMLNFLNETRSFFFANVYPYFSWAANPMNINLDFALFNGNFEQIDGGSGLVY 240

Query: 241 TNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMT 300
           TNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGAN+LNAATYNRNLVK++T
Sbjct: 241 TNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANLLNAATYNRNLVKRIT 300

Query: 301 AKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVE 360
           AKPTVGTPARPGVVIPTFIFALFDENQK G GTERHWGLL SDGSPNY+IDLTGK+SSVE
Sbjct: 301 AKPTVGTPARPGVVIPTFIFALFDENQKPGSGTERHWGLLRSDGSPNYQIDLTGKESSVE 360

Query: 361 YNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSV 420
           Y+P+PVVENN PF+G LWCVAARGV+LMEL AA+  VCG G+GTCEALSPG+ECYEPVSV
Sbjct: 361 YDPLPVVENNAPFRGRLWCVAARGVDLMELEAAVDDVCGRGDGTCEALSPGRECYEPVSV 420

Query: 421 YWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           YWHASYAFSS+WSKFRSQGASCYFNGLAEQTT+DPSNGSC+FPSVTF
Sbjct: 421 YWHASYAFSSYWSKFRSQGASCYFNGLAEQTTVDPSNGSCRFPSVTF 467

BLAST of MC05g0735 vs. ExPASy TrEMBL
Match: A0A5A7VKC4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00070 PE=3 SV=1)

HSP 1 Score: 769 bits (1986), Expect = 2.58e-278
Identity = 370/452 (81.86%), Postives = 410/452 (90.71%), Query Frame = 0

Query: 14  LLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDANPQFLKLLSGTKL 73
           ++I  DAEISSKIG+NYGQLG+NLP PN A+EM+ SMK GRVKLYDAN + L  LS TK 
Sbjct: 1   MMIYIDAEISSKIGVNYGQLGNNLPPPNLAIEMMNSMKVGRVKLYDANHEILGNLSKTKF 60

Query: 74  QVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEVLSYD---DRRVWH 133
           QVSIMIPN++ISDIA NQTRAD+WI +NV+ FYP TMIRFILVGNEVLS+D   DR+VW+
Sbjct: 61  QVSIMIPNNEISDIANNQTRADQWISNNVLPFYPQTMIRFILVGNEVLSFDSDKDRQVWN 120

Query: 134 QLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDIRRSVVAPMLEFLN 193
            LVPAMR IW SLKA NLQ IKVGTPVAMD+LE+ FPPSRGTFRSDI+++VVAPML FLN
Sbjct: 121 DLVPAMRRIWSSLKANNLQTIKVGTPVAMDILETTFPPSRGTFRSDIQQTVVAPMLNFLN 180

Query: 194 ETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTNLLDEMLDSLIFAM 253
           ETRS+FF + YPYFSW+ANP +INLDFALF  NFEQID G+GL YTNLLDEMLDSLIFAM
Sbjct: 181 ETRSFFFANVYPYFSWAANPMNINLDFALFNGNFEQIDEGSGLVYTNLLDEMLDSLIFAM 240

Query: 254 AKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAKPTVGTPARPGVVI 313
           AKLGYPDIRLVISETGWPTAGDIEQPGAN+LNAATYNRNLVK++TAKPTVGTPARPGVVI
Sbjct: 241 AKLGYPDIRLVISETGWPTAGDIEQPGANLLNAATYNRNLVKRITAKPTVGTPARPGVVI 300

Query: 314 PTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNPMPVVENNVPFKG 373
           PTFIFALFDENQK G GTERHWGLL SDGSPNY+IDLTGK+SSVEY+P+PVVENN PF+G
Sbjct: 301 PTFIFALFDENQKPGSGTERHWGLLRSDGSPNYQIDLTGKESSVEYDPLPVVENNAPFRG 360

Query: 374 TLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYWHASYAFSSFWSKF 433
            LWCVAARGV+LMEL AA+  VCG G+GTCEALSPG+ECYEPVSVYWHASYAFSS+WSKF
Sbjct: 361 RLWCVAARGVDLMELEAAVDDVCGRGDGTCEALSPGRECYEPVSVYWHASYAFSSYWSKF 420

Query: 434 RSQGASCYFNGLAEQTTIDPSNGSCKFPSVTF 462
           RSQGASCYFNGLAEQTT+DPSNGSC+FPSVTF
Sbjct: 421 RSQGASCYFNGLAEQTTVDPSNGSCRFPSVTF 452

BLAST of MC05g0735 vs. TAIR 10
Match: AT3G23770.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 581.6 bits (1498), Expect = 5.4e-166
Identity = 282/478 (59.00%), Postives = 354/478 (74.06%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDA--------------EISSKIGINYGQLGSNLPSPNQAVEM 60
           M  FAL+L    +LL LS +               ++S+IGINYG+LG+NLP P Q++ +
Sbjct: 1   MTPFALFLF---TLLALSSSCCSAIGPQNNRTVLALASRIGINYGKLGNNLPFPYQSINL 60

Query: 61  IKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFY 120
           IK++KAG VKLYDA+P+ LKLLS T L V+IM+PN+QI  I A+Q  AD W+  NV+ F+
Sbjct: 61  IKTIKAGHVKLYDADPETLKLLSTTNLYVTIMVPNNQIISIGADQAAADNWVATNVLPFH 120

Query: 121 PDTMIRFILVGNEVLSY---DDRRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLE 180
           P T IRF+LVGNEVLSY    D+++W  LVPAMR +  SL+AR +  IKVGTP+AMD L 
Sbjct: 121 PQTRIRFVLVGNEVLSYSSDQDKQIWANLVPAMRKVVNSLRARGIHNIKVGTPLAMDALR 180

Query: 181 SAFPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEAN 240
           S+FPPS GTFR DI   V+ P+L+FLN T S+FF+D YPYF WS +P + +LDFALFE+N
Sbjct: 181 SSFPPSSGTFREDIAVPVMLPLLKFLNGTNSFFFLDVYPYFPWSTDPVNNHLDFALFESN 240

Query: 241 FEQIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNA 300
               D  TGL YTNLLD+MLDS+IFAM KLGYP+I L ISETGWP  GDI + GANI+NA
Sbjct: 241 STYTDPQTGLVYTNLLDQMLDSVIFAMTKLGYPNISLAISETGWPNDGDIHETGANIVNA 300

Query: 301 ATYNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNY 360
           ATYNRNL+KKMTA P +GTPAR G  IPTF+F+LF+ENQK G GTERHWG+L+ DG+P Y
Sbjct: 301 ATYNRNLIKKMTANPPLGTPARRGAPIPTFLFSLFNENQKPGSGTERHWGILNPDGTPIY 360

Query: 361 EIDLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEAL 420
           +ID +G++S   ++ +P   NNVPFKG +WCVA  G +  ELG A+   CG  N TC AL
Sbjct: 361 DIDFSGRRSFSGFDSLPKPSNNVPFKGNVWCVAVDGADEAELGQALNFACGRSNATCAAL 420

Query: 421 SPGKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVT 462
           +PG ECY PV+V WHASYAFSS+W++FR+Q + CYFNGLA +TT +P N  CKFPSVT
Sbjct: 421 APGGECYAPVTVTWHASYAFSSYWAQFRNQSSQCYFNGLARETTTNPGNERCKFPSVT 475

BLAST of MC05g0735 vs. TAIR 10
Match: AT4G14080.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 571.6 bits (1472), Expect = 5.6e-163
Identity = 261/441 (59.18%), Postives = 334/441 (75.74%), Query Frame = 0

Query: 21  EISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDANPQFLKLLSGTKLQVSIMIP 80
           +++SKIGINYG+ G+NLPSP Q++  IKS+KAG VKLYDA+P+ L LLS T L V+I +P
Sbjct: 37  DLASKIGINYGRRGNNLPSPYQSINFIKSIKAGHVKLYDADPESLTLLSQTNLYVTITVP 96

Query: 81  NDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEVLSYDDRRVWHQLVPAMRSIW 140
           N QI+ +++NQT ADEW+  N++ +YP T IRF+LVGNE+LSY+   V   LVPAMR I 
Sbjct: 97  NHQITALSSNQTIADEWVRTNILPYYPQTQIRFVLVGNEILSYNSGNVSVNLVPAMRKIV 156

Query: 141 RSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDIRRSVVAPMLEFLNETRSYFFVDA 200
            SL+   +  IKVGTP+AMD L S+FPPS GTFR +I   V+ P+L+FLN T SYFF++ 
Sbjct: 157 NSLRLHGIHNIKVGTPLAMDSLRSSFPPSNGTFREEITGPVMLPLLKFLNGTNSYFFLNV 216

Query: 201 YPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTNLLDEMLDSLIFAMAKLGYPDIRL 260
           +PYF WS NP + +LDFALF+ +    D  TGL Y NLLD+MLDS++FAM KLGYP +RL
Sbjct: 217 HPYFRWSRNPMNTSLDFALFQGHSTYTDPQTGLVYRNLLDQMLDSVLFAMTKLGYPHMRL 276

Query: 261 VISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAKPTVGTPARPGVVIPTFIFALFDE 320
            ISETGWP  GDI++ GANILNAATYNRNL+KKM+A P +GTP+RPG+ IPTF+F+LF+E
Sbjct: 277 AISETGWPNFGDIDETGANILNAATYNRNLIKKMSASPPIGTPSRPGLPIPTFVFSLFNE 336

Query: 321 NQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNPMPVVENNVPFKGTLWCVAARGV 380
           NQK G GT+RHWG+L  DGSP Y++D TG+     +NP+P   NNVP+KG +WCV   G 
Sbjct: 337 NQKSGSGTQRHWGILHPDGSPIYDVDFTGQTPLTGFNPLPKPTNNVPYKGQVWCVPVEGA 396

Query: 381 NLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYWHASYAFSSFWSKFRSQGASCYFN 440
           N  EL   +   C   N TC AL+PG+ECYEPVS+YWHASYA +S+W++FR+Q   C+FN
Sbjct: 397 NETELEETLRMACAQSNTTCAALAPGRECYEPVSIYWHASYALNSYWAQFRNQSIQCFFN 456

Query: 441 GLAEQTTIDPSNGSCKFPSVT 462
           GLA +TT +P N  CKFPSVT
Sbjct: 457 GLAHETTTNPGNDRCKFPSVT 477

BLAST of MC05g0735 vs. TAIR 10
Match: AT3G07320.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.4e-145
Identity = 246/457 (53.83%), Postives = 330/457 (72.21%), Query Frame = 0

Query: 6   LYLILLQSLLILSDAEISSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYDANPQFL 65
           L+L  L  L+ +S A+ S + GINYGQLG+NLPSP+ +V +IKS+ A RVKLYDANP+ L
Sbjct: 5   LHLFALSLLISVSGAKFSGRPGINYGQLGNNLPSPSDSVNLIKSLNAKRVKLYDANPKIL 64

Query: 66  KLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNEVLSYDD 125
             L+GT + VS+M+PN+ + +I+ + + +D+WI  N++ FYP T IR++LVGNE+LS  D
Sbjct: 65  AALNGTDITVSVMVPNELLVNISKSASLSDDWIRSNILPFYPTTKIRYLLVGNEILSLPD 124

Query: 126 RRVWHQLVPAMRSIWRSLKARNLQIIKVGTPVAMDVLESAFPPSRGTFRSDIRRSVVAPM 185
             +   LVPAMR I RSLK+  ++ +KVGT +A DVL+S+FPPS G FR DI   ++ PM
Sbjct: 125 SELKSSLVPAMRKIQRSLKSLGVKKVKVGTTLATDVLQSSFPPSSGEFREDISGLIMKPM 184

Query: 186 LEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEA-NFEQIDGGTGLAYTNLLDEMLD 245
           L+FLN T+S+ FVD YPYF+W+ +P  ++LD+A+FE+ N    D  + L Y NL D+M+D
Sbjct: 185 LQFLNRTKSFLFVDVYPYFAWAQDPTHVDLDYAIFESTNVTVTDPVSNLTYHNLFDQMID 244

Query: 246 SLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAKPTVGTPA 305
           + +FAM ++GYPDIR+ ++ETGWP  GD +Q GANI NAATYNRN+VKK+ A P VGTPA
Sbjct: 245 AFVFAMKRVGYPDIRIWVAETGWPNNGDYDQIGANIYNAATYNRNVVKKLAADPPVGTPA 304

Query: 306 RPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNPMPVVEN 365
           RPG V+P F+FAL++ENQK GPGTERH+GLL  +G+  Y IDL+GK    E  P P  EN
Sbjct: 305 RPGKVLPAFVFALYNENQKTGPGTERHFGLLHPNGTQVYGIDLSGKTEYKESLPAP--EN 364

Query: 366 NVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYWHASYAFS 425
           N  +KG +WCV A+G N  +LG A++  C  GN TC+ +  G  C +P     HASYAFS
Sbjct: 365 NDLYKGKIWCVVAKGANWTQLGDALSYACSQGNNTCDPIQRGGPCQKPDLTVLHASYAFS 424

Query: 426 SFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPSVT 462
           S+W++FR  G +C FNGLA QT  DPS G C+FPSVT
Sbjct: 425 SYWAQFRKIGGTCSFNGLATQTIKDPSYGRCEFPSVT 459

BLAST of MC05g0735 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 352.1 bits (902), Expect = 7.0e-97
Identity = 181/461 (39.26%), Postives = 273/461 (59.22%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDAEI-SSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYD 60
           MA F L L++L S   +      S  IG+NYG++  NLP+P + VE++K+    R+KLYD
Sbjct: 1   MAVFVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYD 60

Query: 61  ANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNE 120
                L  L+ + ++V + +PN+ ++  AA+Q+  D W+ DN+  + P T I  I VGNE
Sbjct: 61  TETTVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNE 120

Query: 121 VLSYDDRRVWHQLVPAMRSIWRSLKARNL-QIIKVGTPVAMDVLESAFPPSRGTFRSDIR 180
           V   D R     LVPAM+++  SL   NL + IK+ +P+A+  L S++PPS G+F+ ++ 
Sbjct: 121 VF-VDPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELI 180

Query: 181 RSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTNL 240
             V+ PML+ L +T S+  V+AYP+F+++AN   I+LD+ALF+ N   +D G GL Y +L
Sbjct: 181 EPVIKPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSL 240

Query: 241 LDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAKP 300
           LD  +D++  AM+ +G+ D++LV++ETGWP+AGD  + GA   NAA YN  LVK++    
Sbjct: 241 LDAQIDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGN 300

Query: 301 TVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNP 360
             GTP +P   +  ++FALF+ENQK GP +ER++GL   + +  Y++ L GK + V  N 
Sbjct: 301 --GTPLKPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNK 360

Query: 361 MPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYWH 420
             VV       G  WCVA       +L   +   CG G   C  + PG  CY P S+  H
Sbjct: 361 EKVVPVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESLEAH 420

Query: 421 ASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPSNGSCKFPS 460
           ASYAF+S++ K      +C F G A   +  P  G C+FP+
Sbjct: 421 ASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPKYGKCEFPT 458

BLAST of MC05g0735 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 343.2 bits (879), Expect = 3.3e-94
Identity = 178/453 (39.29%), Postives = 268/453 (59.16%), Query Frame = 0

Query: 1   MATFALYLILLQSLLILSDAEI-SSKIGINYGQLGSNLPSPNQAVEMIKSMKAGRVKLYD 60
           MA F L L++L S   +      S  IG+NYG++  NLP+P + VE++K+    R+KLYD
Sbjct: 1   MAVFVLSLLILSSFSAIPFTYADSGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYD 60

Query: 61  ANPQFLKLLSGTKLQVSIMIPNDQISDIAANQTRADEWILDNVVAFYPDTMIRFILVGNE 120
                L  L+ + ++V + +PN+ ++  AA+Q+  D W+ DN+  + P T I  I VGNE
Sbjct: 61  TETTVLTALANSGIKVVVSLPNENLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNE 120

Query: 121 VLSYDDRRVWHQLVPAMRSIWRSLKARNL-QIIKVGTPVAMDVLESAFPPSRGTFRSDIR 180
           V   D R     LVPAM+++  SL   NL + IK+ +P+A+  L S++PPS G+F+ ++ 
Sbjct: 121 VF-VDPRNTTTYLVPAMKNVQSSLVKFNLDKSIKISSPIALSALASSYPPSAGSFKPELI 180

Query: 181 RSVVAPMLEFLNETRSYFFVDAYPYFSWSANPRDINLDFALFEANFEQIDGGTGLAYTNL 240
             V+ PML+ L +T S+  V+AYP+F+++AN   I+LD+ALF+ N   +D G GL Y +L
Sbjct: 181 EPVIKPMLDLLRKTSSHLMVNAYPFFAYAANADKISLDYALFKENAGNVDSGNGLKYNSL 240

Query: 241 LDEMLDSLIFAMAKLGYPDIRLVISETGWPTAGDIEQPGANILNAATYNRNLVKKMTAKP 300
           LD  +D++  AM+ +G+ D++LV++ETGWP+AGD  + GA   NAA YN  LVK++    
Sbjct: 241 LDAQIDAVFAAMSAVGFNDVKLVVTETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGN 300

Query: 301 TVGTPARPGVVIPTFIFALFDENQKLGPGTERHWGLLSSDGSPNYEIDLTGKKSSVEYNP 360
             GTP +P   +  ++FALF+ENQK GP +ER++GL   + +  Y++ L GK + V  N 
Sbjct: 301 --GTPLKPKEPLNVYLFALFNENQKTGPTSERNYGLFYPNENKVYDVSLNGKSTPVNDNK 360

Query: 361 MPVVENNVPFKGTLWCVAARGVNLMELGAAITTVCGGGNGTCEALSPGKECYEPVSVYWH 420
             VV       G  WCVA       +L   +   CG G   C  + PG  CY P S+  H
Sbjct: 361 EKVVPVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESLEAH 420

Query: 421 ASYAFSSFWSKFRSQGASCYFNGLAEQTTIDPS 452
           ASYAF+S++ K      +C F G A   +  PS
Sbjct: 421 ASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPS 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q069157.9e-16259.18Probable glucan endo-1,3-beta-glucosidase A6 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9FJU91.5e-8335.65Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q9M0691.9e-8338.02Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q8VYE52.8e-8234.76Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9C7U51.8e-8137.04Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ... [more]
Match NameE-valueIdentityDescription
XP_022138211.16.11e-312100.00probable glucan endo-1,3-beta-glucosidase A6 [Momordica charantia][more]
XP_038905649.12.73e-29384.80probable glucan endo-1,3-beta-glucosidase A6 isoform X2 [Benincasa hispida][more]
XP_022944377.11.04e-28582.15probable glucan endo-1,3-beta-glucosidase A6 isoform X2 [Cucurbita moschata][more]
XP_022972257.12.45e-28481.72probable glucan endo-1,3-beta-glucosidase A6 isoform X1 [Cucurbita maxima][more]
XP_023540016.12.45e-28481.94probable glucan endo-1,3-beta-glucosidase A6 isoform X1 [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
A0A6J1C9252.96e-312100.00(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111009439 ... [more]
A0A6J1FVI95.06e-28682.15(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111448824 P... [more]
A0A6J1I4C11.19e-28481.72(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111470842 PE=... [more]
A0A1S3AYG24.27e-28381.58(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103483949 PE=3 SV... [more]
A0A5A7VKC42.58e-27881.86(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT3G23770.15.4e-16659.00O-Glycosyl hydrolases family 17 protein [more]
AT4G14080.15.6e-16359.18O-Glycosyl hydrolases family 17 protein [more]
AT3G07320.11.4e-14553.83O-Glycosyl hydrolases family 17 protein [more]
AT5G55180.17.0e-9739.26O-Glycosyl hydrolases family 17 protein [more]
AT5G55180.23.3e-9439.29O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 372..457
e-value: 3.3E-35
score: 133.0
IPR012946X8 domainPFAMPF07983X8coord: 372..443
e-value: 5.4E-15
score: 55.8
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 26..346
e-value: 1.1E-107
score: 360.3
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 258..271
NoneNo IPR availableGENE3D1.20.58.1040coord: 368..460
e-value: 4.0E-5
score: 25.8
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 24..355
e-value: 2.1E-120
score: 403.5
NoneNo IPR availablePANTHERPTHR32227:SF370GLUCAN ENDO-1,3-BETA-GLUCOSIDASE A6-RELATEDcoord: 7..454
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 7..454
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..346

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC05g0735.1MC05g0735.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds