MC05g0681 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0681
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	GDSL esterase/lipase At4g16230-like
Location	MC05: 5269760 .. 5271422 (+)
RNA-Seq Expression	MC05g0681
Synteny	MC05g0681

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAACATTGTTTTTGGTATTCCATTTTTTATACTATTTGCCTTGTTTGGGACTTGCTTCTCCAAAGCTTTTACAGGTTTTTTTGTGTTTGGGGATTCATTGGTCGAGGTTGGAAACAACAATTACATTCCAACACTCTCCCGAGCGAATTATGTGCCCAATGGCATCGATTTCGGAAGGCCAACTGGTCGATTCACCAATGGGCGAACCATTCTTGACATTATAGGCAAGTTATGAACTTCATTGCAATACCAAAACCAGCAAAAAAACAGTAAAAAGAAGTTCAATGTCTGTTCTGTTTCCCATCTTACCAGGACAGGAGCTTGGCTTCAAGATGTTCACTCCCCCGTACTTGGCCCCTTCCACGACGGGACAGGTGATTCTCCAAGGCGTCAACTATGCTTCTGGTGCAGCTGGAATCTTCAACAAAACAGGAAAAATGTTTGTAAGTTTCTGCTGCAATCCATGGAGTGTGTTTTTTCTATGGAAAATGATGGATGGAATGCACATTTTTATGCTTTGTTTTTGTATATGTATTGTTCTGGTTATGTTTTGTAATGACATTGTGTTGAATGTGAGTTGAAAAAGGGCGGTCGAATCAACATGGATGCACAAATTGATAAGTTTGCAAATACCAGACAAGATATCATTGCCATGATTGGCCTTCCTGCAGCTCTAGATTGGCTAAGAACTTCCATCTTCACGATAGCCATTGGCTCGAACGATTTCATAAACAACTACTTCACACCAGTCCTCTCAGATTCTAGCCACCGGTTAATTCCTCGAGAGTTGTTTGTCGATTCCATGATTTCGAGATTCAGACTCCAGCTCACTGTAATAACACTCTTCCTTGTTGCTTCACAAACATCAAAGATCAAAGTAGATTATTCCTGTCAATTGCTTCATTCGAAAACGGTTCTGAAAAAAATTATCTAAATCTCTTGCAGAGACTTTACCATCTAGGAGCGAGAATGATCCTAGTCGCAAACGTCGGACCAATTGGATGCATTCCAAATCAGAGAGATGCGAATCCTTCATCAGGAAACAGCTGTGCAAATTCCCCTAACCTAGTGGCTCAGTCATTCAACACCAAATTGAGAGGTCTAATCACAGAGCTCAGAACACAATTTGAGGATTCAAATTTCCTTTACGCAGATACATTTCGCATCGTTCAAGATATAACTCAGAATAACGCATCATACGGTGAGAATATTGCGTTAAATTTTTTTCATTGAGGAAATTAGTCGAATACCTGCGATTGTGCTTCACAATTGACAGCTTCGCTCAATCGCTCGCTGTGTTTGCATAGGATTTGAGAACGCCGACTCGGCGTGCTGCCACATTGCCGGCCGCCACGGCGGTCTTTTTCCCTGTGGACCGCCGTCGAAAGTGTGCGGAGACAGATCGAAGTACGTGTTTTGGGATCCATATCATCCTTCTGAAGCTGCAAATTCCATAATCGCGCGGAGGCTCTTGGAAGGCGACGCCATTGATATTTGGCCGATCAATATTCGAGAACTTGAACGTCTTATTAATTCGTAGTTCTTTTCTTTTCTTCGTATTTTATTTTCTTTTGAATAAATGTTAATTAGTAATTAATCTGAAACTAGACGCATGGTTTTTGTCCGTTTGTTGAACATTTTATCTTGGTTAGATCTACCCG

mRNA sequence

AAGAACATTGTTTTTGGTATTCCATTTTTTATACTATTTGCCTTGTTTGGGACTTGCTTCTCCAAAGCTTTTACAGGTTTTTTTGTGTTTGGGGATTCATTGGTCGAGGTTGGAAACAACAATTACATTCCAACACTCTCCCGAGCGAATTATGTGCCCAATGGCATCGATTTCGGAAGGCCAACTGGTCGATTCACCAATGGGCGAACCATTCTTGACATTATAGGCAAGCTTGGCTTCAAGATGTTCACTCCCCCGTACTTGGCCCCTTCCACGACGGGACAGGTGATTCTCCAAGGCGTCAACTATGCTTCTGGTGCAGCTGGAATCTTCAACAAAACAGGAAAAATGTTTGGCGGTCGAATCAACATGGATGCACAAATTGATAAGTTTGCAAATACCAGACAAGATATCATTGCCATGATTGGCCTTCCTGCAGCTCTAGATTGGCTAAGAACTTCCATCTTCACGATAGCCATTGGCTCGAACGATTTCATAAACAACTACTTCACACCAGTCCTCTCAGATTCTAGCCACCGGTTAATTCCTCGAGAGTTGTTTGTCGATTCCATGATTTCGAGATTCAGACTCCAGCTCACTAGACTTTACCATCTAGGAGCGAGAATGATCCTAGTCGCAAACGTCGGACCAATTGGATGCATTCCAAATCAGAGAGATGCGAATCCTTCATCAGGAAACAGCTGTGCAAATTCCCCTAACCTAGTGGCTCAGTCATTCAACACCAAATTGAGAGGTCTAATCACAGAGCTCAGAACACAATTTGAGGATTCAAATTTCCTTTACGCAGATACATTTCGCATCGTTCAAGATATAACTCAGAATAACGCATCATACGGATTTGAGAACGCCGACTCGGCGTGCTGCCACATTGCCGGCCGCCACGGCGGTCTTTTTCCCTGTGGACCGCCGTCGAAAGTGTGCGGAGACAGATCGAAGTACGTGTTTTGGGATCCATATCATCCTTCTGAAGCTGCAAATTCCATAATCGCGCGGAGGCTCTTGGAAGGCGACGCCATTGATATTTGGCCGATCAATATTCGAGAACTTGAACGTCTTATTAATTCGTAGTTCTTTTCTTTTCTTCGTATTTTATTTTCTTTTGAATAAATGTTAATTAGTAATTAATCTGAAACTAGACGCATGGTTTTTGTCCGTTTGTTGAACATTTTATCTTGGTTAGATCTACCCG

Coding sequence (CDS)

AAGAACATTGTTTTTGGTATTCCATTTTTTATACTATTTGCCTTGTTTGGGACTTGCTTCTCCAAAGCTTTTACAGGTTTTTTTGTGTTTGGGGATTCATTGGTCGAGGTTGGAAACAACAATTACATTCCAACACTCTCCCGAGCGAATTATGTGCCCAATGGCATCGATTTCGGAAGGCCAACTGGTCGATTCACCAATGGGCGAACCATTCTTGACATTATAGGCAAGCTTGGCTTCAAGATGTTCACTCCCCCGTACTTGGCCCCTTCCACGACGGGACAGGTGATTCTCCAAGGCGTCAACTATGCTTCTGGTGCAGCTGGAATCTTCAACAAAACAGGAAAAATGTTTGGCGGTCGAATCAACATGGATGCACAAATTGATAAGTTTGCAAATACCAGACAAGATATCATTGCCATGATTGGCCTTCCTGCAGCTCTAGATTGGCTAAGAACTTCCATCTTCACGATAGCCATTGGCTCGAACGATTTCATAAACAACTACTTCACACCAGTCCTCTCAGATTCTAGCCACCGGTTAATTCCTCGAGAGTTGTTTGTCGATTCCATGATTTCGAGATTCAGACTCCAGCTCACTAGACTTTACCATCTAGGAGCGAGAATGATCCTAGTCGCAAACGTCGGACCAATTGGATGCATTCCAAATCAGAGAGATGCGAATCCTTCATCAGGAAACAGCTGTGCAAATTCCCCTAACCTAGTGGCTCAGTCATTCAACACCAAATTGAGAGGTCTAATCACAGAGCTCAGAACACAATTTGAGGATTCAAATTTCCTTTACGCAGATACATTTCGCATCGTTCAAGATATAACTCAGAATAACGCATCATACGGATTTGAGAACGCCGACTCGGCGTGCTGCCACATTGCCGGCCGCCACGGCGGTCTTTTTCCCTGTGGACCGCCGTCGAAAGTGTGCGGAGACAGATCGAAGTACGTGTTTTGGGATCCATATCATCCTTCTGAAGCTGCAAATTCCATAATCGCGCGGAGGCTCTTGGAAGGCGACGCCATTGATATTTGGCCGATCAATATTCGAGAACTTGAACGTCTTATTAATTCGTAG

Protein sequence

KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGRPTGRFTNGRTILDIIGKLGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERLINS

Homology

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BLAST of MC05g0681 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 449.9 bits (1156), Expect = 2.7e-125
Identity = 222/358 (62.01%), Postives = 269/358 (75.14%), Query Frame = 0


Query: 3   IVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGRPT 62
           IV  + FF    L G    K     FVFGDSLV+ GNNNY+ TLS+ANYVPNGIDFG PT
Sbjct: 11  IVLSVLFFSEVCLAG----KKIPANFVFGDSLVDAGNNNYLATLSKANYVPNGIDFGSPT 70

Query: 63  GRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGR 122
           GRFTNGRTI+DI+ + LG    TPPYLAP+T+G +IL GVNYASG +GI N TGK+FG R
Sbjct: 71  GRFTNGRTIVDIVYQALGSDELTPPYLAPTTSGSLILNGVNYASGGSGILNSTGKLFGER 130

Query: 123 INMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHRL 182
           IN+DAQ+D FA TRQDII+ IG   A    R++IF++  GSND INNYFTPV+S    ++
Sbjct: 131 INVDAQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVISTLQRKV 190

Query: 183 IPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNL 242
           +  E+FVD+MIS+FRLQLTRLY LGAR I+V N+GPIGCIP +R+++P++GN+C   PN 
Sbjct: 191 VAPEVFVDTMISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAAGNNCLAEPNE 250

Query: 243 VAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAGRH 302
           VAQ +N KL+ L+ EL    + S F+Y D FRIV DI QN +SYGFE+    CC + G+ 
Sbjct: 251 VAQMYNLKLKTLVEELNKNLQGSRFVYGDVFRIVDDIIQNYSSYGFESEKIPCCSLVGKV 310

Query: 303 GGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 360
           GGL PCGPPSKVC DRSKYVFWDPYHP+EAAN IIARRLL GD  DI+PINIR+L  L
Sbjct: 311 GGLIPCGPPSKVCMDRSKYVFWDPYHPTEAANIIIARRLLSGDTSDIYPINIRQLANL 364

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BLAST of MC05g0681 vs. ExPASy Swiss-Prot
Match: Q9M2R9 (GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 1.9e-94
Identity = 180/374 (48.13%), Postives = 237/374 (63.37%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFS--------KAFTGFFVFGDSLVEVGNNNYIPTLSRANYV 60
           K  +F +P  +LF  FG+ FS        +A    FVFGDSLV+ GNNNY+ TLSRAN  
Sbjct: 2   KKSIFFVPVLVLF-FFGSRFSRVASAGDQRALAASFVFGDSLVDAGNNNYLQTLSRANSP 61

Query: 61  PNGIDF----GRPTGRFTNGRTILDIIG-KLGFKMFTPPYLAPSTTGQVILQGVNYASGA 120
           PNGIDF    G PTGRFTNGRTI DI+G KLG + +  PYLAP+ +G+ +L GVNYASG 
Sbjct: 62  PNGIDFKPSRGNPTGRFTNGRTIADIVGEKLGQQSYAVPYLAPNASGEALLNGVNYASGG 121

Query: 121 AGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMIGLPAALDWLR-TSIFTIAIGSNDFI 180
            GI N TG +F  R+ MD Q+D F NTR+    ++G   A D++R  S+F++ IGSNDF+
Sbjct: 122 GGILNATGSVFVNRLGMDIQVDYFTNTRKQFDKLLGQDKARDYIRKRSLFSVVIGSNDFL 181

Query: 181 NNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRD 240
           NNY  P ++  +      E FVD MIS  R QL RLY + AR  +V NV PIGCIP Q+ 
Sbjct: 182 NNYLVPFVAAQARLTQTPETFVDDMISHLRNQLKRLYDMDARKFVVGNVAPIGCIPYQKS 241

Query: 241 ANPSSGNSCANSPNLVAQSFNTKLRGLIT-ELRTQFEDSNFLYADTFRIVQDITQNNASY 300
            N  +   C +  N +A  +N +L+ L+T EL+   +D++F+YA+ + +  D+  N   Y
Sbjct: 242 INQLNDKQCVDLANKLAIQYNARLKDLLTVELKDSLKDAHFVYANVYDLFMDLIVNFKDY 301

Query: 301 GFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDA 360
           GF  A  ACC   GR  G+ PCGP S +C DRSK+VFWD YHP+EAAN +IA +LL GD+
Sbjct: 302 GFRTASEACCETRGRLAGILPCGPTSSLCTDRSKHVFWDAYHPTEAANLLIADKLLYGDS 361

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BLAST of MC05g0681 vs. ExPASy Swiss-Prot
Match: O80470 (GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.7e-90
Identity = 164/335 (48.96%), Postives = 221/335 (65.97%), Query Frame = 0


Query: 28  FVFGDSLVEVGNNNYIPTLSRANYVPNGIDF----GRPTGRFTNGRTILDIIG-KLGFKM 87
           F+FGDSLV+ GNNNY+ TLSRAN  PNGIDF    G PTGRFTNGRTI DI+G +LG   
Sbjct: 50  FIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTNGRTIGDIVGEELGSAN 109

Query: 88  FTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMI 147
           +  P+LAP   G+ +L GVNYASG  GI N TG++F  R+ MD Q+D F  TR+    ++
Sbjct: 110 YAIPFLAPDAKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDVQVDFFNTTRKQFDDLL 169

Query: 148 GLPAALDWL-RTSIFTIAIGSNDFINNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTR 207
           G   A D++ + SIF+I IG+NDF+NNY  P+LS  +      + F+  M+   R QLTR
Sbjct: 170 GKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPDDFIGDMLEHLRDQLTR 229

Query: 208 LYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNLVAQSFNTKLRGLITELRTQF 267
           LY L AR  ++ NVGPIGCIP Q+  N    N C +  N +A  +N +L+ L+ EL  + 
Sbjct: 230 LYQLDARKFVIGNVGPIGCIPYQKTINQLDENECVDLANKLANQYNVRLKSLLEELNKKL 289

Query: 268 EDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYV 327
             + F++A+ + +V ++  N   YGF++A  ACC   G++ G+ PCGP S +C +R KYV
Sbjct: 290 PGAMFVHANVYDLVMELITNYDKYGFKSATKACCGNGGQYAGIIPCGPTSSLCEERDKYV 349

Query: 328 FWDPYHPSEAANSIIARRLLEGDAIDIWPINIREL 357
           FWDPYHPSEAAN IIA++LL GD   I P+N+ +L
Sbjct: 350 FWDPYHPSEAANVIIAKQLLYGDVKVISPVNLSKL 384

[Close]

BLAST of MC05g0681 vs. ExPASy Swiss-Prot
Match: Q9C7N5 (GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.8e-75
Identity = 143/336 (42.56%), Postives = 209/336 (62.20%), Query Frame = 0


Query: 27  FFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGRPTGRFTNGRTILDIIGK-LGFKMFTP 86
           +F+FGDSLV+ GNNN + +++RA+Y P GIDFG PTGRF+NGRT +D++ + LGF  + P
Sbjct: 33  YFIFGDSLVDNGNNNRLRSIARADYFPYGIDFGGPTGRFSNGRTTVDVLTELLGFDNYIP 92

Query: 87  PYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMIGLP 146
            Y   + +GQ ILQGVNYAS AAGI  +TG   G RI    Q++ + NT   ++ ++G  
Sbjct: 93  AY--STVSGQEILQGVNYASAAAGIREETGAQLGQRITFSGQVENYKNTVAQVVEILGDE 152

Query: 147 -AALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTRLYH 206
             A D+L+  I+++ +GSND++NNYF P    +S +  P E + D +ISR+R QL  LY+
Sbjct: 153 YTAADYLKRCIYSVGMGSNDYLNNYFMPQFYSTSRQYTP-EQYADDLISRYRDQLNALYN 212

Query: 207 LGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNLVAQSFNTKLRGLITELRTQFEDS 266
            GAR   +  +G IGC PN        G +C    N   + FN +L  ++ +L     D+
Sbjct: 213 YGARKFALVGIGAIGCSPNALAQGSQDGTTCVERINSANRIFNNRLISMVQQLNNAHSDA 272

Query: 267 NFLYADTFRIVQDITQNNASYGFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYVFWD 326
           +F Y + +   QDI  N ++YGF N ++ACC I GR+GG   C P    C +R +YVFWD
Sbjct: 273 SFTYINAYGAFQDIIANPSAYGFTNTNTACCGI-GRNGGQLTCLPGEPPCLNRDEYVFWD 332

Query: 327 PYHPSEAANSIIARRLLEGD-AIDIWPINIRELERL 360
            +HPS AAN+ IA+R      + D++PI+I +L +L
Sbjct: 333 AFHPSAAANTAIAKRSYNAQRSSDVYPIDISQLAQL 364

[Close]

BLAST of MC05g0681 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.8e-75
Identity = 145/336 (43.15%), Postives = 209/336 (62.20%), Query Frame = 0


Query: 27  FFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGRPTGRFTNGRTILDIIGK-LGFKMFTP 86
           FFVFGDSLV+ GNNN + +++R+NY P GIDFG PTGRF+NG+T +D+I + LGF  + P
Sbjct: 33  FFVFGDSLVDNGNNNGLISIARSNYFPYGIDFGGPTGRFSNGKTTVDVIAELLGFNGYIP 92

Query: 87  PYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMIG-L 146
            Y   + +G+ IL GVNYAS AAGI  +TG+  G RI+   Q+  +  T   ++ ++G  
Sbjct: 93  AY--NTVSGRQILSGVNYASAAAGIREETGRQLGQRISFSGQVRNYQTTVSQVVQLLGDE 152

Query: 147 PAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTRLYH 206
             A D+L+  I+++ +GSND++NNYF P    SS +  P E + + +ISR+  QL  LY+
Sbjct: 153 TRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQFTP-EQYANDLISRYSTQLNALYN 212

Query: 207 LGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNLVAQSFNTKLRGLITELRTQFEDS 266
            GAR   ++ +G +GC PN    +P  G +C +  N   Q FN KLR L+ +L     D+
Sbjct: 213 YGARKFALSGIGAVGCSPNALAGSP-DGRTCVDRINSANQIFNNKLRSLVDQLNNNHPDA 272

Query: 267 NFLYADTFRIVQDITQNNASYGFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYVFWD 326
            F+Y + + I QD+  N A +GF   ++ CC I GR+ G   C P  + C DR+ YVFWD
Sbjct: 273 KFIYINAYGIFQDMITNPARFGFRVTNAGCCGI-GRNAGQITCLPGQRPCRDRNAYVFWD 332

Query: 327 PYHPSEAANSIIARRLLEG-DAIDIWPINIRELERL 360
            +HP+EAAN IIARR      A D +P++I  L +L
Sbjct: 333 AFHPTEAANVIIARRSYNAQSASDAYPMDISRLAQL 363

[Close]

BLAST of MC05g0681 vs. NCBI nr
Match: XP_022138052.1 (GDSL esterase/lipase At4g16230-like [Momordica charantia])

HSP 1 Score: 726 bits (1875), Expect = 3.66e-264
Identity = 361/363 (99.45%), Postives = 362/363 (99.72%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 60
           KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR
Sbjct: 8   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 67

Query: 61  PTGRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 120
           PTGRFTNGRTILDIIG+ LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG
Sbjct: 68  PTGRFTNGRTILDIIGQELGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 127

Query: 121 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 180
           GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH
Sbjct: 128 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 187

Query: 181 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 240
           RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP
Sbjct: 188 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 247

Query: 241 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 300
           NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG
Sbjct: 248 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 307

Query: 301 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 360
           RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL
Sbjct: 308 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 367

Query: 361 INS 362
           INS
Sbjct: 368 INS 370

[Close]

BLAST of MC05g0681 vs. NCBI nr
Match: XP_008438962.1 (PREDICTED: GDSL esterase/lipase At4g16230-like [Cucumis melo])

HSP 1 Score: 597 bits (1539), Expect = 5.34e-213
Identity = 296/360 (82.22%), Postives = 322/360 (89.44%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 60
           KN V GI  F LF LFGTCFS+AFT  FVFGDSLVEVGNNNYIP+LSRANYVPNGIDFGR
Sbjct: 8   KNTVLGILCFTLFTLFGTCFSRAFTANFVFGDSLVEVGNNNYIPSLSRANYVPNGIDFGR 67

Query: 61  PTGRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 120
           PTGRFTNGRTI+DIIG+ LGFK FTPPYLAPSTTG VI++G+NYASG+AGI N TGK+F 
Sbjct: 68  PTGRFTNGRTIVDIIGQELGFKTFTPPYLAPSTTGPVIIRGINYASGSAGILNNTGKIFI 127

Query: 121 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 180
            RINMDAQID FANTRQDII MIGLP+A+D LRTSIF+I IGSNDFINNYFTPVLSDS H
Sbjct: 128 ARINMDAQIDNFANTRQDIITMIGLPSAIDLLRTSIFSITIGSNDFINNYFTPVLSDSGH 187

Query: 181 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 240
           RLIP ELFV SMISR+RLQLTRLY+LGAR I+V NVGPIGCIP QRD+NPS GN+CANSP
Sbjct: 188 RLIPPELFVGSMISRYRLQLTRLYNLGARRIVVVNVGPIGCIPYQRDSNPSLGNNCANSP 247

Query: 241 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 300
           NL+AQ FN++LRGL+TEL  +F+DSNFLYAD F IVQDI QN+ASYGFENADSACCHIAG
Sbjct: 248 NLMAQLFNSQLRGLLTELGARFQDSNFLYADAFHIVQDIVQNHASYGFENADSACCHIAG 307

Query: 301 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 359
           R+GGLFPCGPPS VC DRSKYVFWD +HPSEAANSIIA RLL GDA DIWPINIRELERL
Sbjct: 308 RYGGLFPCGPPSSVCVDRSKYVFWDSFHPSEAANSIIAGRLLNGDADDIWPINIRELERL 367

[Close]

BLAST of MC05g0681 vs. NCBI nr
Match: XP_004134507.1 (GDSL esterase/lipase At4g16230 [Cucumis sativus] >KAE8650406.1 hypothetical protein Csa_010475 [Cucumis sativus])

HSP 1 Score: 595 bits (1535), Expect = 2.17e-212
Identity = 294/360 (81.67%), Postives = 323/360 (89.72%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 60
           KN V GI  F LF LFGTCFS+AFT  FVFGDSLVEVGNNNYIP+LSRANYVPNGIDFGR
Sbjct: 8   KNTVLGILCFTLFTLFGTCFSRAFTANFVFGDSLVEVGNNNYIPSLSRANYVPNGIDFGR 67

Query: 61  PTGRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 120
           PTGRFTNGRTI+DIIG+ LGFK FTPPY+APSTTG+VIL+G+NYASG+AGI N TGK+F 
Sbjct: 68  PTGRFTNGRTIVDIIGQELGFKTFTPPYMAPSTTGRVILRGINYASGSAGILNNTGKIFI 127

Query: 121 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 180
            RINMDAQID FANTRQDII MIGL +A+D LRTSIF+I IGSNDFINNYFTPVLSDS H
Sbjct: 128 ARINMDAQIDNFANTRQDIITMIGLHSAIDLLRTSIFSITIGSNDFINNYFTPVLSDSGH 187

Query: 181 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 240
           RLIP ELFV SMISR+RLQLTRLY+LGAR I+V NVGPIGCIP QRD+NPS GN+CANSP
Sbjct: 188 RLIPPELFVGSMISRYRLQLTRLYNLGARRIVVVNVGPIGCIPYQRDSNPSLGNNCANSP 247

Query: 241 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 300
           NL+AQ FN++LRGL+TEL ++F+D NFLYAD F IVQDI QN+ASYGFENADSACCHIAG
Sbjct: 248 NLMAQLFNSQLRGLLTELGSRFQDGNFLYADAFHIVQDIVQNHASYGFENADSACCHIAG 307

Query: 301 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 359
           R+GGLFPCGPPS VC DRSKYVFWD +HPSEAANSIIA RLL GDA+DIWPINIRELERL
Sbjct: 308 RYGGLFPCGPPSSVCVDRSKYVFWDSFHPSEAANSIIAGRLLNGDAVDIWPINIRELERL 367

[Close]

BLAST of MC05g0681 vs. NCBI nr
Match: XP_038881806.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At4g16230-like [Benincasa hispida])

HSP 1 Score: 576 bits (1484), Expect = 1.27e-204
Identity = 289/360 (80.28%), Postives = 311/360 (86.39%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 60
           KN V GI  F LF LFGTCFS+ FT  FVFGDSLVEVGNNNYIP+LSRANYVPNGIDFGR
Sbjct: 8   KNTVEGILCFTLFTLFGTCFSRDFTANFVFGDSLVEVGNNNYIPSLSRANYVPNGIDFGR 67

Query: 61  PTGRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 120
           PTGRFTNGRTI+DIIG+ LGF  FT PYLAPSTTG VI  GVNYASG+AGI N  GK+F 
Sbjct: 68  PTGRFTNGRTIVDIIGQELGFMTFTTPYLAPSTTGAVIFXGVNYASGSAGILNNAGKIFI 127

Query: 121 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 180
            RINMDAQID  ANTRQDII+MIGLPAA+D LRTSIF+I IGSNDFINNYFTPVLSDS H
Sbjct: 128 ARINMDAQIDNLANTRQDIISMIGLPAAIDLLRTSIFSITIGSNDFINNYFTPVLSDSGH 187

Query: 181 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 240
           RLIP EL V SMISR+RLQLTRLY+LGAR I+  NVGPIGCIP QRD+ PS GN+CANSP
Sbjct: 188 RLIPPELSVGSMISRYRLQLTRLYNLGARRIVAVNVGPIGCIPYQRDSKPSLGNNCANSP 247

Query: 241 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 300
           NL+AQ FN +LRGL+ EL  +F+DSNFLYAD FRIVQDI QN+ASYGFENADSACCHIAG
Sbjct: 248 NLMAQLFNNQLRGLLIELGARFQDSNFLYADAFRIVQDIVQNHASYGFENADSACCHIAG 307

Query: 301 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 359
           R+GGLFPCGPPS VC DRSKYVFWD +HPSE ANS IA RLL GDAIDIWPINIRELERL
Sbjct: 308 RYGGLFPCGPPSSVCVDRSKYVFWDSFHPSETANSXIAGRLLNGDAIDIWPINIRELERL 367

[Close]

BLAST of MC05g0681 vs. NCBI nr
Match: XP_023528521.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At4g16230-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 574 bits (1479), Expect = 7.31e-204
Identity = 285/359 (79.39%), Postives = 315/359 (87.74%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 60
            N V GI  F LF LFGTCFS+AFT  FVFGDSLVEVGNNNYIP+LSRANYVPNGIDFG 
Sbjct: 8   NNTVLGILCFTLFTLFGTCFSRAFTANFVFGDSLVEVGNNNYIPSLSRANYVPNGIDFGW 67

Query: 61  PTGRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 120
           PTGRFTNGRTI+DIIG+ LGF+ FTPPYLAPS+TG VIL GVNYASG+AGIFN TGK+F 
Sbjct: 68  PTGRFTNGRTIVDIIGQELGFETFTPPYLAPSSTGPVILHGVNYASGSAGIFNNTGKIFL 127

Query: 121 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 180
            RIN+ AQ+D FAN RQDII MIGLPAA+D LRTSIF+I IGSNDFINNYFTPVLSD  H
Sbjct: 128 ARINLVAQVDNFANNRQDIIDMIGLPAAMDLLRTSIFSITIGSNDFINNYFTPVLSDXGH 187

Query: 181 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 240
           RLIP ELFV SMIS +RLQLTRLY+LGAR I+V NVGPIGCIP QRD+NPS G++CANSP
Sbjct: 188 RLIPPELFVGSMISSYRLQLTRLYNLGARNIVVVNVGPIGCIPYQRDSNPSLGSNCANSP 247

Query: 241 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 300
           NL+AQ  N +LRGLITEL  QF++SNFLYA+ + IVQDI QN+ASYGFENADSACCHIAG
Sbjct: 248 NLMAQLLNNQLRGLITELGAQFQNSNFLYANAYHIVQDIVQNHASYGFENADSACCHIAG 307

Query: 301 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELER 358
           R+GGLFPCGPPS+VC DRSKYVFWD +HPSEA NSIIARRL++GDA DIWPINIRELER
Sbjct: 308 RYGGLFPCGPPSRVCVDRSKYVFWDSFHPSEATNSIIARRLMDGDANDIWPINIRELER 366

[Close]

BLAST of MC05g0681 vs. ExPASy TrEMBL
Match: A0A6J1C9Z0 (GDSL esterase/lipase At4g16230-like OS=Momordica charantia OX=3673 GN=LOC111009310 PE=3 SV=1)

HSP 1 Score: 726 bits (1875), Expect = 1.77e-264
Identity = 361/363 (99.45%), Postives = 362/363 (99.72%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 60
           KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR
Sbjct: 8   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 67

Query: 61  PTGRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 120
           PTGRFTNGRTILDIIG+ LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG
Sbjct: 68  PTGRFTNGRTILDIIGQELGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 127

Query: 121 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 180
           GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH
Sbjct: 128 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 187

Query: 181 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 240
           RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP
Sbjct: 188 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 247

Query: 241 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 300
           NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG
Sbjct: 248 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 307

Query: 301 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 360
           RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL
Sbjct: 308 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 367

Query: 361 INS 362
           INS
Sbjct: 368 INS 370

[Close]

BLAST of MC05g0681 vs. ExPASy TrEMBL
Match: A0A1S3AYA3 (GDSL esterase/lipase At4g16230-like OS=Cucumis melo OX=3656 GN=LOC103483897 PE=3 SV=1)

HSP 1 Score: 597 bits (1539), Expect = 2.59e-213
Identity = 296/360 (82.22%), Postives = 322/360 (89.44%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 60
           KN V GI  F LF LFGTCFS+AFT  FVFGDSLVEVGNNNYIP+LSRANYVPNGIDFGR
Sbjct: 8   KNTVLGILCFTLFTLFGTCFSRAFTANFVFGDSLVEVGNNNYIPSLSRANYVPNGIDFGR 67

Query: 61  PTGRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFG 120
           PTGRFTNGRTI+DIIG+ LGFK FTPPYLAPSTTG VI++G+NYASG+AGI N TGK+F 
Sbjct: 68  PTGRFTNGRTIVDIIGQELGFKTFTPPYLAPSTTGPVIIRGINYASGSAGILNNTGKIFI 127

Query: 121 GRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSH 180
            RINMDAQID FANTRQDII MIGLP+A+D LRTSIF+I IGSNDFINNYFTPVLSDS H
Sbjct: 128 ARINMDAQIDNFANTRQDIITMIGLPSAIDLLRTSIFSITIGSNDFINNYFTPVLSDSGH 187

Query: 181 RLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSP 240
           RLIP ELFV SMISR+RLQLTRLY+LGAR I+V NVGPIGCIP QRD+NPS GN+CANSP
Sbjct: 188 RLIPPELFVGSMISRYRLQLTRLYNLGARRIVVVNVGPIGCIPYQRDSNPSLGNNCANSP 247

Query: 241 NLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAG 300
           NL+AQ FN++LRGL+TEL  +F+DSNFLYAD F IVQDI QN+ASYGFENADSACCHIAG
Sbjct: 248 NLMAQLFNSQLRGLLTELGARFQDSNFLYADAFHIVQDIVQNHASYGFENADSACCHIAG 307

Query: 301 RHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 359
           R+GGLFPCGPPS VC DRSKYVFWD +HPSEAANSIIA RLL GDA DIWPINIRELERL
Sbjct: 308 RYGGLFPCGPPSSVCVDRSKYVFWDSFHPSEAANSIIAGRLLNGDADDIWPINIRELERL 367

[Close]

BLAST of MC05g0681 vs. ExPASy TrEMBL
Match: A0A6J1GYI0 (LOW QUALITY PROTEIN: GDSL esterase/lipase At4g16230-like OS=Cucurbita moschata OX=3662 GN=LOC111458001 PE=3 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 3.32e-198
Identity = 283/360 (78.61%), Postives = 315/360 (87.50%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPN-GIDFG 60
            N V GI  F LF LFGT FS+AFT  FVFGDSLVEVGNNNYIP+LSRAN VPN GIDFG
Sbjct: 87  NNTVLGILCFTLFTLFGTRFSRAFTANFVFGDSLVEVGNNNYIPSLSRANNVPNLGIDFG 146

Query: 61  RPTGRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMF 120
            PTGRFTN RTI+DIIG+ LGF+ FTPP+LAPS+TG +IL+GVNYASG+AGIFN TGK+F
Sbjct: 147 WPTGRFTNRRTIVDIIGQELGFETFTPPHLAPSSTGPMILRGVNYASGSAGIFNNTGKIF 206

Query: 121 GGRINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSS 180
             RIN+ AQ+D FAN RQDII MIGLPAA+D LRTSIF+I IGSNDFINNYFTPVLSDS 
Sbjct: 207 LARINLVAQVDNFANNRQDIIDMIGLPAAMDLLRTSIFSITIGSNDFINNYFTPVLSDSG 266

Query: 181 HRLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANS 240
           HRLIP ELFV SMIS +RLQLTRLY+LGAR I+V NVGPIGCIP QRD+NPS G++CANS
Sbjct: 267 HRLIPPELFVGSMISSYRLQLTRLYNLGARNIVVVNVGPIGCIPYQRDSNPSLGSNCANS 326

Query: 241 PNLVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIA 300
           PNL+AQ FN +LRGLITEL  QF++SNFLYA+ + IVQDI QN+ASYGFENADSACCHIA
Sbjct: 327 PNLMAQLFNNQLRGLITELGAQFQNSNFLYANAYHIVQDIVQNHASYGFENADSACCHIA 386

Query: 301 GRHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELER 358
           GRHGGLFPCGPPS+VC DRSKYVFWD +HPSEA NSIIARRL++GDA DIWPINIRELER
Sbjct: 387 GRHGGLFPCGPPSRVCVDRSKYVFWDSFHPSEATNSIIARRLMDGDANDIWPINIRELER 446

[Close]

BLAST of MC05g0681 vs. ExPASy TrEMBL
Match: A0A0A0L961 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G167360 PE=3 SV=1)

HSP 1 Score: 538 bits (1387), Expect = 1.29e-190
Identity = 271/359 (75.49%), Postives = 299/359 (83.29%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGR 60
           KN V GI  F LF LFGTCFS+AFT  FVFGDSLVEVGNNNYIP+LSRANYVPNGIDFGR
Sbjct: 8   KNTVLGILCFTLFTLFGTCFSRAFTANFVFGDSLVEVGNNNYIPSLSRANYVPNGIDFGR 67

Query: 61  PTGRFTNGRTILDIIGKLGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGG 120
           PTG+            +LGFK FTPPY+APSTTG+VIL+G+NY +               
Sbjct: 68  PTGQ------------ELGFKTFTPPYMAPSTTGRVILRGINYIA--------------- 127

Query: 121 RINMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHR 180
           RINMDAQID FANTRQDII MIGL +A+D LRTSIF+I IGSNDFINNYFTPVLSDS HR
Sbjct: 128 RINMDAQIDNFANTRQDIITMIGLHSAIDLLRTSIFSITIGSNDFINNYFTPVLSDSGHR 187

Query: 181 LIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSPN 240
           LIP ELFV SMISR+RLQLTRLY+LGAR I+V NVGPIGCIP QRD+NPS GN+CANSPN
Sbjct: 188 LIPPELFVGSMISRYRLQLTRLYNLGARRIVVVNVGPIGCIPYQRDSNPSLGNNCANSPN 247

Query: 241 LVAQSFNTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAGR 300
           L+AQ FN++LRGL+TEL ++F+D NFLYAD F IVQDI QN+ASYGFENADSACCHIAGR
Sbjct: 248 LMAQLFNSQLRGLLTELGSRFQDGNFLYADAFHIVQDIVQNHASYGFENADSACCHIAGR 307

Query: 301 HGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 359
           +GGLFPCGPPS VC DRSKYVFWD +HPSEAANSIIA RLL GDA+DIWPINIRELERL
Sbjct: 308 YGGLFPCGPPSSVCVDRSKYVFWDSFHPSEAANSIIAGRLLNGDAVDIWPINIRELERL 339

[Close]

BLAST of MC05g0681 vs. ExPASy TrEMBL
Match: A0A5N6RCN4 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_015306 PE=3 SV=1)

HSP 1 Score: 498 bits (1282), Expect = 3.36e-174
Identity = 241/353 (68.27%), Postives = 285/353 (80.74%), Query Frame = 0


Query: 15  LFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGRPTGRFTNGRTILDI 74
           L G CF+      FVFGDSLV+ GNNNYI +LS+ANYVPNG+DFG PTGR+TNGRTI+DI
Sbjct: 16  LVGICFANNIPASFVFGDSLVDTGNNNYIVSLSKANYVPNGVDFGMPTGRYTNGRTIVDI 75

Query: 75  IG--------KLGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGRINMDA 134
           IG        +LG K FTPPYLAP+T G V+LQGVNYASG  GI N TGK+FGGRIN+DA
Sbjct: 76  IGNIFFLAGQELGLKDFTPPYLAPTTAGPVLLQGVNYASGGGGILNGTGKIFGGRINLDA 135

Query: 135 QIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHRLIPREL 194
           QID FANTRQDII+ +G PAAL    TS+F++ IGSNDFINNY TPVLS    +LI  E+
Sbjct: 136 QIDNFANTRQDIISSVGAPAALKLFGTSLFSVTIGSNDFINNYLTPVLSAPERKLISPEM 195

Query: 195 FVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNLVAQSF 254
           FVD++ISR+RLQLTRLY+LGAR I+VANVGPIGCIP QRD+NP++G++C + PN +AQ F
Sbjct: 196 FVDAVISRYRLQLTRLYNLGARKIVVANVGPIGCIPYQRDSNPAAGDNCVSLPNQLAQQF 255

Query: 255 NTKLRGLITELRTQFEDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAGRHGGLFP 314
           NT+L+ L+TEL T  + S F YAD + IV DI QN  SYGFENA+SACC++AGRHGGL P
Sbjct: 256 NTQLKSLVTELNTDLQGSQFAYADVYHIVDDILQNFMSYGFENANSACCYVAGRHGGLIP 315

Query: 315 CGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDAIDIWPINIRELERL 359
           CGPPSKVCGDRSKYVFWDPYHPS+A+N IIA RLL+GD+ DI PINIR+L  L
Sbjct: 316 CGPPSKVCGDRSKYVFWDPYHPSDASNVIIAGRLLDGDSNDITPINIRQLAGL 368

[Close]

BLAST of MC05g0681 vs. TAIR 10
Match: AT3G50400.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 347.4 bits (890), Expect = 1.4e-95
Identity = 180/374 (48.13%), Postives = 237/374 (63.37%), Query Frame = 0


Query: 1   KNIVFGIPFFILFALFGTCFS--------KAFTGFFVFGDSLVEVGNNNYIPTLSRANYV 60
           K  +F +P  +LF  FG+ FS        +A    FVFGDSLV+ GNNNY+ TLSRAN  
Sbjct: 2   KKSIFFVPVLVLF-FFGSRFSRVASAGDQRALAASFVFGDSLVDAGNNNYLQTLSRANSP 61

Query: 61  PNGIDF----GRPTGRFTNGRTILDIIG-KLGFKMFTPPYLAPSTTGQVILQGVNYASGA 120
           PNGIDF    G PTGRFTNGRTI DI+G KLG + +  PYLAP+ +G+ +L GVNYASG 
Sbjct: 62  PNGIDFKPSRGNPTGRFTNGRTIADIVGEKLGQQSYAVPYLAPNASGEALLNGVNYASGG 121

Query: 121 AGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMIGLPAALDWLR-TSIFTIAIGSNDFI 180
            GI N TG +F  R+ MD Q+D F NTR+    ++G   A D++R  S+F++ IGSNDF+
Sbjct: 122 GGILNATGSVFVNRLGMDIQVDYFTNTRKQFDKLLGQDKARDYIRKRSLFSVVIGSNDFL 181

Query: 181 NNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRD 240
           NNY  P ++  +      E FVD MIS  R QL RLY + AR  +V NV PIGCIP Q+ 
Sbjct: 182 NNYLVPFVAAQARLTQTPETFVDDMISHLRNQLKRLYDMDARKFVVGNVAPIGCIPYQKS 241

Query: 241 ANPSSGNSCANSPNLVAQSFNTKLRGLIT-ELRTQFEDSNFLYADTFRIVQDITQNNASY 300
            N  +   C +  N +A  +N +L+ L+T EL+   +D++F+YA+ + +  D+  N   Y
Sbjct: 242 INQLNDKQCVDLANKLAIQYNARLKDLLTVELKDSLKDAHFVYANVYDLFMDLIVNFKDY 301

Query: 301 GFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYVFWDPYHPSEAANSIIARRLLEGDA 360
           GF  A  ACC   GR  G+ PCGP S +C DRSK+VFWD YHP+EAAN +IA +LL GD+
Sbjct: 302 GFRTASEACCETRGRLAGILPCGPTSSLCTDRSKHVFWDAYHPTEAANLLIADKLLYGDS 361

[Close]

BLAST of MC05g0681 vs. TAIR 10
Match: AT2G23540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 2.6e-91
Identity = 164/335 (48.96%), Postives = 221/335 (65.97%), Query Frame = 0


Query: 28  FVFGDSLVEVGNNNYIPTLSRANYVPNGIDF----GRPTGRFTNGRTILDIIG-KLGFKM 87
           F+FGDSLV+ GNNNY+ TLSRAN  PNGIDF    G PTGRFTNGRTI DI+G +LG   
Sbjct: 50  FIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTNGRTIGDIVGEELGSAN 109

Query: 88  FTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMI 147
           +  P+LAP   G+ +L GVNYASG  GI N TG++F  R+ MD Q+D F  TR+    ++
Sbjct: 110 YAIPFLAPDAKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDVQVDFFNTTRKQFDDLL 169

Query: 148 GLPAALDWL-RTSIFTIAIGSNDFINNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTR 207
           G   A D++ + SIF+I IG+NDF+NNY  P+LS  +      + F+  M+   R QLTR
Sbjct: 170 GKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPDDFIGDMLEHLRDQLTR 229

Query: 208 LYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNLVAQSFNTKLRGLITELRTQF 267
           LY L AR  ++ NVGPIGCIP Q+  N    N C +  N +A  +N +L+ L+ EL  + 
Sbjct: 230 LYQLDARKFVIGNVGPIGCIPYQKTINQLDENECVDLANKLANQYNVRLKSLLEELNKKL 289

Query: 268 EDSNFLYADTFRIVQDITQNNASYGFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYV 327
             + F++A+ + +V ++  N   YGF++A  ACC   G++ G+ PCGP S +C +R KYV
Sbjct: 290 PGAMFVHANVYDLVMELITNYDKYGFKSATKACCGNGGQYAGIIPCGPTSSLCEERDKYV 349

Query: 328 FWDPYHPSEAANSIIARRLLEGDAIDIWPINIREL 357
           FWDPYHPSEAAN IIA++LL GD   I P+N+ +L
Sbjct: 350 FWDPYHPSEAANVIIAKQLLYGDVKVISPVNLSKL 384

[Close]

BLAST of MC05g0681 vs. TAIR 10
Match: AT4G16230.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 291.6 bits (745), Expect = 8.8e-79
Identity = 147/241 (61.00%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 3   IVFGIPFFILFALFGTCFSKAFTGFFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGRPT 62
           IV  + FF    L G    K     FVFGDSLV+ GNNNY+ TLS+ANYVPNGIDFG PT
Sbjct: 11  IVLSVLFFSEVCLAG----KKIPANFVFGDSLVDAGNNNYLATLSKANYVPNGIDFGSPT 70

Query: 63  GRFTNGRTILDIIGK-LGFKMFTPPYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGR 122
           GRFTNGRTI+DI+ + LG    TPPYLAP+T+G +IL GVNYASG +GI N TGK+FG R
Sbjct: 71  GRFTNGRTIVDIVYQALGSDELTPPYLAPTTSGSLILNGVNYASGGSGILNSTGKLFGER 130

Query: 123 INMDAQIDKFANTRQDIIAMIGLPAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHRL 182
           IN+DAQ+D FA TRQDII+ IG   A    R++IF++  GSND INNYFTPV+S    ++
Sbjct: 131 INVDAQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVISTLQRKV 190

Query: 183 IPRELFVDSMISRFRLQLTRLYHLGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNL 242
           +  E+FVD+MIS+FRLQLTRLY LGAR I+V N+GPIGCIP +R+++P++GN+C   PN 
Sbjct: 191 VAPEVFVDTMISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAAGNNCLAEPNE 247

[Close]

BLAST of MC05g0681 vs. TAIR 10
Match: AT1G29670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 7.0e-76
Identity = 145/336 (43.15%), Postives = 209/336 (62.20%), Query Frame = 0


Query: 27  FFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGRPTGRFTNGRTILDIIGK-LGFKMFTP 86
           FFVFGDSLV+ GNNN + +++R+NY P GIDFG PTGRF+NG+T +D+I + LGF  + P
Sbjct: 33  FFVFGDSLVDNGNNNGLISIARSNYFPYGIDFGGPTGRFSNGKTTVDVIAELLGFNGYIP 92

Query: 87  PYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMIG-L 146
            Y   + +G+ IL GVNYAS AAGI  +TG+  G RI+   Q+  +  T   ++ ++G  
Sbjct: 93  AY--NTVSGRQILSGVNYASAAAGIREETGRQLGQRISFSGQVRNYQTTVSQVVQLLGDE 152

Query: 147 PAALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTRLYH 206
             A D+L+  I+++ +GSND++NNYF P    SS +  P E + + +ISR+  QL  LY+
Sbjct: 153 TRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQFTP-EQYANDLISRYSTQLNALYN 212

Query: 207 LGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNLVAQSFNTKLRGLITELRTQFEDS 266
            GAR   ++ +G +GC PN    +P  G +C +  N   Q FN KLR L+ +L     D+
Sbjct: 213 YGARKFALSGIGAVGCSPNALAGSP-DGRTCVDRINSANQIFNNKLRSLVDQLNNNHPDA 272

Query: 267 NFLYADTFRIVQDITQNNASYGFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYVFWD 326
            F+Y + + I QD+  N A +GF   ++ CC I GR+ G   C P  + C DR+ YVFWD
Sbjct: 273 KFIYINAYGIFQDMITNPARFGFRVTNAGCCGI-GRNAGQITCLPGQRPCRDRNAYVFWD 332

Query: 327 PYHPSEAANSIIARRLLEG-DAIDIWPINIRELERL 360
            +HP+EAAN IIARR      A D +P++I  L +L
Sbjct: 333 AFHPTEAANVIIARRSYNAQSASDAYPMDISRLAQL 363

[Close]

BLAST of MC05g0681 vs. TAIR 10
Match: AT1G29660.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 7.0e-76
Identity = 143/336 (42.56%), Postives = 209/336 (62.20%), Query Frame = 0


Query: 27  FFVFGDSLVEVGNNNYIPTLSRANYVPNGIDFGRPTGRFTNGRTILDIIGK-LGFKMFTP 86
           +F+FGDSLV+ GNNN + +++RA+Y P GIDFG PTGRF+NGRT +D++ + LGF  + P
Sbjct: 33  YFIFGDSLVDNGNNNRLRSIARADYFPYGIDFGGPTGRFSNGRTTVDVLTELLGFDNYIP 92

Query: 87  PYLAPSTTGQVILQGVNYASGAAGIFNKTGKMFGGRINMDAQIDKFANTRQDIIAMIGLP 146
            Y   + +GQ ILQGVNYAS AAGI  +TG   G RI    Q++ + NT   ++ ++G  
Sbjct: 93  AY--STVSGQEILQGVNYASAAAGIREETGAQLGQRITFSGQVENYKNTVAQVVEILGDE 152

Query: 147 -AALDWLRTSIFTIAIGSNDFINNYFTPVLSDSSHRLIPRELFVDSMISRFRLQLTRLYH 206
             A D+L+  I+++ +GSND++NNYF P    +S +  P E + D +ISR+R QL  LY+
Sbjct: 153 YTAADYLKRCIYSVGMGSNDYLNNYFMPQFYSTSRQYTP-EQYADDLISRYRDQLNALYN 212

Query: 207 LGARMILVANVGPIGCIPNQRDANPSSGNSCANSPNLVAQSFNTKLRGLITELRTQFEDS 266
            GAR   +  +G IGC PN        G +C    N   + FN +L  ++ +L     D+
Sbjct: 213 YGARKFALVGIGAIGCSPNALAQGSQDGTTCVERINSANRIFNNRLISMVQQLNNAHSDA 272

Query: 267 NFLYADTFRIVQDITQNNASYGFENADSACCHIAGRHGGLFPCGPPSKVCGDRSKYVFWD 326
           +F Y + +   QDI  N ++YGF N ++ACC I GR+GG   C P    C +R +YVFWD
Sbjct: 273 SFTYINAYGAFQDIIANPSAYGFTNTNTACCGI-GRNGGQLTCLPGEPPCLNRDEYVFWD 332

Query: 327 PYHPSEAANSIIARRLLEGD-AIDIWPINIRELERL 360
            +HPS AAN+ IA+R      + D++PI+I +L +L
Sbjct: 333 AFHPSAAANTAIAKRSYNAQRSSDVYPIDISQLAQL 364

The following BLAST results are available for this feature:

BLAST of MC05g0681 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23470	2.7e-125	62.01	GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3...	[more]
Q9M2R9	1.9e-94	48.13	GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2...	[more]
O80470	3.7e-90	48.96	GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2...	[more]
Q9C7N5	9.8e-75	42.56	GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1...	[more]
Q9C7N4	9.8e-75	43.15	GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2...	[more]

BLAST of MC05g0681 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138052.1	3.66e-264	99.45	GDSL esterase/lipase At4g16230-like [Momordica charantia]	[more]
XP_008438962.1	5.34e-213	82.22	PREDICTED: GDSL esterase/lipase At4g16230-like [Cucumis melo]	[more]
XP_004134507.1	2.17e-212	81.67	GDSL esterase/lipase At4g16230 [Cucumis sativus] >KAE8650406.1 hypothetical prot...	[more]
XP_038881806.1	1.27e-204	80.28	LOW QUALITY PROTEIN: GDSL esterase/lipase At4g16230-like [Benincasa hispida]	[more]
XP_023528521.1	7.31e-204	79.39	LOW QUALITY PROTEIN: GDSL esterase/lipase At4g16230-like [Cucurbita pepo subsp. ...	[more]

BLAST of MC05g0681 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C9Z0	1.77e-264	99.45	GDSL esterase/lipase At4g16230-like OS=Momordica charantia OX=3673 GN=LOC1110093...	[more]
A0A1S3AYA3	2.59e-213	82.22	GDSL esterase/lipase At4g16230-like OS=Cucumis melo OX=3656 GN=LOC103483897 PE=3...	[more]
A0A6J1GYI0	3.32e-198	78.61	LOW QUALITY PROTEIN: GDSL esterase/lipase At4g16230-like OS=Cucurbita moschata O...	[more]
A0A0A0L961	1.29e-190	75.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G167360 PE=3 SV=1	[more]
A0A5N6RCN4	3.36e-174	68.27	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_015306 PE=3 SV=1	[more]

BLAST of MC05g0681 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50400.1	1.4e-95	48.13	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT2G23540.1	2.6e-91	48.96	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT4G16230.1	8.8e-79	61.00	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G29670.1	7.0e-76	43.15	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G29660.1	7.0e-76	42.56	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 20..343 e-value: 4.0E-76 score: 258.5
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 28..339 e-value: 1.9E-28 score: 100.5
None	No IPR available	PANTHER	PTHR45650:SF4	GDSL-LIKE LIPASE/ACYLHYDROLASE SUPERFAMILY PROTEIN	coord: 13..357
None	No IPR available	PANTHER	PTHR45650	GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED	coord: 13..357
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 144..341
IPR035669	GDSL lipase/esterase-like, plant	CDD	cd01837	SGNH_plant_lipase_like	coord: 27..344 e-value: 5.07516E-134 score: 383.119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0681.1	MC05g0681.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds