MC05g0412 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0412
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Auxin-responsive GH3 family protein
Location	MC05: 3089904 .. 3093956 (+)
RNA-Seq Expression	MC05g0412
Synteny	MC05g0412

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGAAATAACCCGACCCGAGAAGAGTACAGAATCCGGCAGCTCCACCCACCGGCCAGAAAGGGGGCGGTAATTGGAGTGGAGAGGCAGGGGTAGAATGGGGATTTGAGCGCCGTATTTGGACGGCATCGGCCACCGGGGGAAGGCCTATAAATATATTTGACTGCCCGTTAGAATCTTCCATCGCATCCACTTCCGTGTCTCTCTCATTTGCAGCCAAGTAAAGCTCTGTAATCTCTCTCTTTCTCTCTCTAGAACCTTGAGAGAGGGACGGTCGTCTTCCATTGGTGCGTTCAGTAACAAGCTTCGTACCTGTTTCTGCAGATTTTTTTGATACTTTGTCCACCAAAAGTCTCCGTCGACTGTTCCGAAGCCTTGTTCTGCGTCACTCTCCAGTAGTTTCCATTTCCAGTACGGTTTCAGGTAATTTGTTTTTCGATTTCTCGAGTGAACTTGCAAGGGCACGAGTGTTTTCCGGTCGATTTTTTTTCTTGATTTACTGTGCCCTGGATGCGAATACCGCGGCGTTTGTTGGAATTTGGAATGTTTAATTTAGTTTGATTCTATGTATCAAATGAATTCGGATTGTTTCTTCTTCTTCTAGTTCCTTGGTTTGCTGCGTTCTGTTCTGCGTGCTCTGTTCTTTAATCCGTTTTTGCCTTTCTTCCTCTGAGTCTTTTTTTTCTCTGAAAAGTCTATGTTATTTTAATCCTCGTTGACGACTTCCTACCTGTAACCTGCCTTTTTTGTCCACAGGAGTTAATTTTTCTAAGTTACTTGTTTGTTTAAATTTTACATTTGAATTGTGGGTTTTTTTAATGTCATTTGGTCGGTCCTGAAAACGGTCTGGGTTGCAGTTGCAGTCTAGTTCTGTTCGTGGGTTTCTGCTGTATTTTCCTGGAAAATTTTTCGAAGGTTGACTTGGTTGAATTCCACCAGAAAACTTTAGATCTTCGAGCAACGTTTGTGCTTCTGCTTTGAGAAGTCTTCAATCCAATCAATCAGTTCCTCTCGCTGTGATTGCTTTTTCTCAAGGTTTCTGTTCAAATGTTGACTTAGATGCATATCCACGAGTTTGCTACATATTCTCTCTTACTTTTCTTTCCTCTTGGGTTTTGCAATAAATTTCAATTCCCAAATAAAATTTTCCGGATGCTTGTAGTTTCCTTTATCTTATCTCCTAGACCCCTTCTGTCTTTTTTGTCCATCTCTCAATTTTCTTACGTAAACATTTCGTTATTCACGTTTTGTGTCTAATCTTTATCTTTAATGCCTTATGGAGCAGTTTGGAGCCCTATGCTGTCTTACAAATATGCTTGGCTTGATATAAATCTGACCTCCGTTCACTCCGTTGTTGTTTTTATGCAACAGTTCGCCGTCCGGCAAGAATAATGTTGGATAAAATGGAAGCATTTGATGGGGAGAAAGTGATAGAGCAATTCGAGGAGATGACCAAGGATGCAGAAAGAGTTCAGAGGGAGACTCTTGAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAGCTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCCCTTGTTACTCACAATGATTTGGAGCCATATATTCAGAGAATCGCGGATGGCGCCACTTCCCCCATTCTCACCGGCAAACCGATCAAAACCATTTCATTGAGGTACTTTTTGCAAATTTGTGCTGAGGAAGTTTTGCTTTTGATTCCAAGTATTGGAGATTTTGTTGCTGTTCCTTTTCTGAGGAGATTCTTGTTTTGTTGGCTGTATGATGTAGCTCTGGTACTACTCAGGGGAGGCCCAAGTTGATTCCCTTCAACGATGAACTCTTGGAGACGACAATGCAAATATATCGCACTTCTTTTGCCTTCAGAAACAAGTAATGGAACTTCTTTTCTGTTCTTGTGTCTCCTGTTTGGACGTTGTTTGTAGTCTGGCCTCTGAATCATTTCTTGTCTGAAATGAACATGGTTAATGCAGTTTGTTGTCTTTTTGACTGTCGGTTGTCATCTTAATAGTATAAAAATGGGCTTGTCATGGCTGATGTGGGGAGAGTTTTTTTGTTCTACAGAGAATTTCCCATTAGGAATGGAAAGGCCTTGCAGTTCATCTACAGCAGCAAGCAGTTCAAAACCAAAGGGGGTCTGGCAGCTGGAACTGCAACAACAAATGTTTACCGCAGTGCGAAGTTTAAGTGCACGATGAAAGCGATCCAGTCGCTGTGCTGTAGCCCGGACGAAGTCATCTTTGGCCCCGACTTCCACCAGTCACTGTATTGCCATCTCTTGTGTGGGCTGATCTTCCGCGATGAAGTTGAGTTTGTGTTCTCCACATTTGCACACAGCATTGTCCATGCTTTCAGGACTTTTGAGCTAGTCTGGGAAGAACTCTGCAGCAACATTAGAGATGGTGTTCTATCTAGTTGGGTCACCGCCCCCACCATTCGGGCAGCTATGTCGAAATTGCTTAGGCCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGCGCAGGGTTGAGTAACTGGTATGGACTGATACCGGAGCTCTTTCCTAACGCAAAGTACATTTACGGGATCATGACCGGGTCGATGGAGCCTTATCTGAAGAAACTGAGGCACTACGCAGGTGATCTGCTGCTGATGAGTGCAGATTATGGTTCTTCAGAAGGATGGGTTGGAGCAAATGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTGTGGTTCTTCCGAACATCGGATACTTCGAGTTCATCCCATTAAAGGAGAGTGCTGAAGAACAGAAGCAGAACGAAGATGGAGCTTTGGTTGCAGCAGAGAACAAGCCAGTGGGTCTGACTGAAGTCAAGGTTGGTGAAGAGTACGAAATTATTGTCACCAATGTCGCAGGTAATTCCTTTCAATTCATGACATTATGCGCTATAACCCTGTTTTGTTTCCTCCAAACCAAACATGAATGAATAGGATGACTGGGTGACAGTTGTGCTCCCAAAATATATTATTGACTCACCATCCAATTCATATGTTATGTTGGATTATTAAAATCTCTTGTTTGTTTCAATGCTCCCCATGTGACCTTGGGGGGGTCTGCAGGATCAATCTCTGCCTCTTGCTTTGTCTTCTTCTGTCCTACGTGTCGTCGAGACGTTGAATTGTCCACTTTAAACCTAAACCCGCTTGATAAAACCGAATTTATTTTCATTCGAACTGTACTCTAGAAGTCTCCACTGGCATTAATGTAGATATTAACTATGGACATTGCATTATAGGGCTGTACCGTTACAGACTGGGGGATGTGGTGAAGGTGATGGGGTTCCATAACGCGACCCCAGAGGTGAAATTCGTGTGCAGGAGGAACCTTCTGCTTAGCATCAACATCGACAAGAACACGGAGAAGGACCTGCAGGTGGCGGTCGAGGCAGCCGGGAGCCTGCTGGCGGAGGAGAAGCTGGAGGTGGTGGACTTCACGAGCCATGTCGACATGTCGAGAGAGCCAGGGCACTACGTGATATTCTGGGAGACGAGCGGAGAGGCGAAGGGGGAGGTCCTGGGGGAGTGCTGCAACTGCCTGGACCGGGCGTTCCTGGACGCAGGGTACGTGAGCTCGAGGAAGGTGAATGCCATTGGAGCACTGGAGCTGAGGGTGGTCCGGAAGGGGACCTTTCACAAGATCATGGACCACTATTTGTCGCTAGGGGCCGCTGTGAGCCAATACAAAACACCCCGTTGTGTTATCCCCACCAACACAACTGTGTTGCAGATCTTGTGCGCCAATGTCGTTAACTCCTATTTCAGTACTGCCTATTAGTTTTTCTTTTACTTCTCCCCACACCATGTGCTCTCTCTCTCTTGTATAATGTAATATTCAAATTCTAGTTATAAGCTTTCCTTTTTCTACCTTTTAAAAATCAACCCAAATATAGAATCTTCACCGTCTAATCTAAATAGGGTTTAAATATCATCAACATGAACTTAGTTCAGCTGTAATTGGCATCTACCTTTGACCATGAGCTCACGTGTTCGAATCCTTCA

mRNA sequence

GTGAAATAACCCGACCCGAGAAGAGTACAGAATCCGGCAGCTCCACCCACCGGCCAGAAAGGGGGCGGTAATTGGAGTGGAGAGGCAGGGGTAGAATGGGGATTTGAGCGCCGTATTTGGACGGCATCGGCCACCGGGGGAAGGCCTATAAATATATTTGACTGCCCGTTAGAATCTTCCATCGCATCCACTTCCGTGTCTCTCTCATTTGCAGCCAAGTAAAGCTCTGTAATCTCTCTCTTTCTCTCTCTAGAACCTTGAGAGAGGGACGGTCGTCTTCCATTGGTGCGTTCAGTAACAAGCTTCGTACCTGTTTCTGCAGATTTTTTTGATACTTTGTCCACCAAAAGTCTCCGTCGACTGTTCCGAAGCCTTGTTCTGCGTCACTCTCCAGTAGTTTCCATTTCCATTCGCCGTCCGGCAAGAATAATGTTGGATAAAATGGAAGCATTTGATGGGGAGAAAGTGATAGAGCAATTCGAGGAGATGACCAAGGATGCAGAAAGAGTTCAGAGGGAGACTCTTGAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAGCTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCCCTTGTTACTCACAATGATTTGGAGCCATATATTCAGAGAATCGCGGATGGCGCCACTTCCCCCATTCTCACCGGCAAACCGATCAAAACCATTTCATTGAGCTCTGGTACTACTCAGGGGAGGCCCAAGTTGATTCCCTTCAACGATGAACTCTTGGAGACGACAATGCAAATATATCGCACTTCTTTTGCCTTCAGAAACAAAGAATTTCCCATTAGGAATGGAAAGGCCTTGCAGTTCATCTACAGCAGCAAGCAGTTCAAAACCAAAGGGGGTCTGGCAGCTGGAACTGCAACAACAAATGTTTACCGCAGTGCGAAGTTTAAGTGCACGATGAAAGCGATCCAGTCGCTGTGCTGTAGCCCGGACGAAGTCATCTTTGGCCCCGACTTCCACCAGTCACTGTATTGCCATCTCTTGTGTGGGCTGATCTTCCGCGATGAAGTTGAGTTTGTGTTCTCCACATTTGCACACAGCATTGTCCATGCTTTCAGGACTTTTGAGCTAGTCTGGGAAGAACTCTGCAGCAACATTAGAGATGGTGTTCTATCTAGTTGGGTCACCGCCCCCACCATTCGGGCAGCTATGTCGAAATTGCTTAGGCCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGCGCAGGGTTGAGTAACTGGTATGGACTGATACCGGAGCTCTTTCCTAACGCAAAGTACATTTACGGGATCATGACCGGGTCGATGGAGCCTTATCTGAAGAAACTGAGGCACTACGCAGGTGATCTGCTGCTGATGAGTGCAGATTATGGTTCTTCAGAAGGATGGGTTGGAGCAAATGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTGTGGTTCTTCCGAACATCGGATACTTCGAGTTCATCCCATTAAAGGAGAGTGCTGAAGAACAGAAGCAGAACGAAGATGGAGCTTTGGTTGCAGCAGAGAACAAGCCAGTGGGTCTGACTGAAGTCAAGGTTGGTGAAGAGTACGAAATTATTGTCACCAATGTCGCAGGGCTGTACCGTTACAGACTGGGGGATGTGGTGAAGGTGATGGGGTTCCATAACGCGACCCCAGAGGTGAAATTCGTGTGCAGGAGGAACCTTCTGCTTAGCATCAACATCGACAAGAACACGGAGAAGGACCTGCAGGTGGCGGTCGAGGCAGCCGGGAGCCTGCTGGCGGAGGAGAAGCTGGAGGTGGTGGACTTCACGAGCCATGTCGACATGTCGAGAGAGCCAGGGCACTACGTGATATTCTGGGAGACGAGCGGAGAGGCGAAGGGGGAGGTCCTGGGGGAGTGCTGCAACTGCCTGGACCGGGCGTTCCTGGACGCAGGGTACGTGAGCTCGAGGAAGGTGAATGCCATTGGAGCACTGGAGCTGAGGGTGGTCCGGAAGGGGACCTTTCACAAGATCATGGACCACTATTTGTCGCTAGGGGCCGCTGTGAGCCAATACAAAACACCCCGTTGTGTTATCCCCACCAACACAACTGTGTTGCAGATCTTGTGCGCCAATGTCGTTAACTCCTATTTCAGTACTGCCTATTAGTTTTTCTTTTACTTCTCCCCACACCATGTGCTCTCTCTCTCTTGTATAATGTAATATTCAAATTCTAGTTATAAGCTTTCCTTTTTCTACCTTTTAAAAATCAACCCAAATATAGAATCTTCACCGTCTAATCTAAATAGGGTTTAAATATCATCAACATGAACTTAGTTCAGCTGTAATTGGCATCTACCTTTGACCATGAGCTCACGTGTTCGAATCCTTCA

Coding sequence (CDS)

ATGTTGGATAAAATGGAAGCATTTGATGGGGAGAAAGTGATAGAGCAATTCGAGGAGATGACCAAGGATGCAGAAAGAGTTCAGAGGGAGACTCTTGAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAGCTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCCCTTGTTACTCACAATGATTTGGAGCCATATATTCAGAGAATCGCGGATGGCGCCACTTCCCCCATTCTCACCGGCAAACCGATCAAAACCATTTCATTGAGCTCTGGTACTACTCAGGGGAGGCCCAAGTTGATTCCCTTCAACGATGAACTCTTGGAGACGACAATGCAAATATATCGCACTTCTTTTGCCTTCAGAAACAAAGAATTTCCCATTAGGAATGGAAAGGCCTTGCAGTTCATCTACAGCAGCAAGCAGTTCAAAACCAAAGGGGGTCTGGCAGCTGGAACTGCAACAACAAATGTTTACCGCAGTGCGAAGTTTAAGTGCACGATGAAAGCGATCCAGTCGCTGTGCTGTAGCCCGGACGAAGTCATCTTTGGCCCCGACTTCCACCAGTCACTGTATTGCCATCTCTTGTGTGGGCTGATCTTCCGCGATGAAGTTGAGTTTGTGTTCTCCACATTTGCACACAGCATTGTCCATGCTTTCAGGACTTTTGAGCTAGTCTGGGAAGAACTCTGCAGCAACATTAGAGATGGTGTTCTATCTAGTTGGGTCACCGCCCCCACCATTCGGGCAGCTATGTCGAAATTGCTTAGGCCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGCGCAGGGTTGAGTAACTGGTATGGACTGATACCGGAGCTCTTTCCTAACGCAAAGTACATTTACGGGATCATGACCGGGTCGATGGAGCCTTATCTGAAGAAACTGAGGCACTACGCAGGTGATCTGCTGCTGATGAGTGCAGATTATGGTTCTTCAGAAGGATGGGTTGGAGCAAATGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTGTGGTTCTTCCGAACATCGGATACTTCGAGTTCATCCCATTAAAGGAGAGTGCTGAAGAACAGAAGCAGAACGAAGATGGAGCTTTGGTTGCAGCAGAGAACAAGCCAGTGGGTCTGACTGAAGTCAAGGTTGGTGAAGAGTACGAAATTATTGTCACCAATGTCGCAGGGCTGTACCGTTACAGACTGGGGGATGTGGTGAAGGTGATGGGGTTCCATAACGCGACCCCAGAGGTGAAATTCGTGTGCAGGAGGAACCTTCTGCTTAGCATCAACATCGACAAGAACACGGAGAAGGACCTGCAGGTGGCGGTCGAGGCAGCCGGGAGCCTGCTGGCGGAGGAGAAGCTGGAGGTGGTGGACTTCACGAGCCATGTCGACATGTCGAGAGAGCCAGGGCACTACGTGATATTCTGGGAGACGAGCGGAGAGGCGAAGGGGGAGGTCCTGGGGGAGTGCTGCAACTGCCTGGACCGGGCGTTCCTGGACGCAGGGTACGTGAGCTCGAGGAAGGTGAATGCCATTGGAGCACTGGAGCTGAGGGTGGTCCGGAAGGGGACCTTTCACAAGATCATGGACCACTATTTGTCGCTAGGGGCCGCTGTGAGCCAATACAAAACACCCCGTTGTGTTATCCCCACCAACACAACTGTGTTGCAGATCTTGTGCGCCAATGTCGTTAACTCCTATTTCAGTACTGCCTATTAG

Protein sequence

MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTMQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY

Homology

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BLAST of MC05g0412 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 2.6e-266
Identity = 441/584 (75.51%), Postives = 512/584 (87.67%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           +++K E FD E+VIE+FE +TKDA ++Q ETL+KILEENG  EYLQ  GLNG+TD  SFK
Sbjct: 4   VVEKTEKFDPEEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQWGLNGKTDSLSFK 63

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM 120
           +C+P+VTH DLEPYI RIADG  SPILTGKPI TISLSSGTTQG+PK +PFN+EL+E+TM
Sbjct: 64  NCIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTM 123

Query: 121 QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ 180
           QI++TSF FRN+EFP+ NGKALQFIY SKQFKTKGGLAAGTATTNVYR+A+FK TMKA+Q
Sbjct: 124 QIFKTSFVFRNREFPVVNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMQ 183

Query: 181 SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS 240
           + CCSPDEVIFGPDF QSLYCHLLCGLIFRDEV+ V STFAHSIVHAFR FE +W+EL +
Sbjct: 184 TPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELVT 243

Query: 241 NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIR+GVLSS V  P++RAAMSKLL+P+PELAD I+ KC+ LSNWYGLIPELFPN +YIYG
Sbjct: 244 NIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRLSNWYGLIPELFPNTRYIYG 303

Query: 301 IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF 360
           IMTGSMEPYLKKLRHYAGDL L+SADYGSSEGW+GANVNP LPPE+ T+ VLPNIGYFEF
Sbjct: 304 IMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYFEF 363

Query: 361 IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG 420
           IPL E       N DG     E  PVGLTEVK+GEEYEI+VTN AGLYRYRLGDVVK+ G
Sbjct: 364 IPLME-------NLDG----LEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGDVVKIKG 423

Query: 421 FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE 480
           FHN TPE++F+CRRNLLLSINIDKNTEKDLQ+AVEAA  +L++EKLEVVDFTSHV++S +
Sbjct: 424 FHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEKLEVVDFTSHVNVSAD 483

Query: 481 PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI 540
           PGHYVIFWE +GEA  E+L ECCNCLD++F+DAGYV SRKV+AIGALELR+V++GTFHKI
Sbjct: 484 PGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVKRGTFHKI 543

Query: 541 MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
           +DH++ LGAAVSQ+KTPRCV PTN +VLQIL +NVV SYFSTA+
Sbjct: 544 LDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

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BLAST of MC05g0412 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 917.5 bits (2370), Expect = 7.4e-266
Identity = 444/585 (75.90%), Postives = 512/585 (87.52%), Query Frame = 0


Query: 1   MLDKME-AFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           +++K+E  FD EKVIE+FE++TKDA ++Q ETL+KILE+NG  EYLQ  GLNGRTDPQ+F
Sbjct: 4   LVEKIEKKFDQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLWGLNGRTDPQTF 63

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120
           K+CVP+VTHNDLEPYIQRIADG  SPILTGKPI+TISLSSGTTQG+PK +PFNDEL+E+T
Sbjct: 64  KNCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMEST 123

Query: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAI 180
           MQI++TSFAFRN+EFPI NGKALQFIYSSKQFKTKGGLAAGTATTNVYR+A+FK TMKA+
Sbjct: 124 MQIFKTSFAFRNREFPIGNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAM 183

Query: 181 QSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELC 240
            + CCSPDEVIFGPDFHQSLYCHLLCGLIF DEV+ V STFAHSIVHAFRTFE VWE L 
Sbjct: 184 CTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALV 243

Query: 241 SNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IR+GVLSS VT P+IR AMSKLL+P+PELAD IY KC+ LSNWYGLIP+LFPN +YIY
Sbjct: 244 VDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRLSNWYGLIPDLFPNTRYIY 303

Query: 301 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360
           GIMTGSMEPYLKKLRHYAG+L L+SADYGSSEGWVG NVNP LPPE+ T+ VLPNIGYFE
Sbjct: 304 GIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGYFE 363

Query: 361 FIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVM 420
           FIPL  +    +Q          N PVGLTEVK+GEEYE++ TN AGLYRYRLGDVVKV 
Sbjct: 364 FIPLGGNLNGIEQ---------ANSPVGLTEVKLGEEYEVVFTNFAGLYRYRLGDVVKVK 423

Query: 421 GFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSR 480
           GFHN TPE++FVCR NLLLSINIDKNTEKDLQ+AVEAA   L +EKLEVVDFTSHV++S 
Sbjct: 424 GFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEKLEVVDFTSHVNVSA 483

Query: 481 EPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHK 540
           +PGHYVIFWE SGEA  E+L +CCNCLDR+F+DAGYVSSRKVNAIGALELR+V++GTFHK
Sbjct: 484 DPGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRIVKRGTFHK 543

Query: 541 IMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
           I+DH++ LG AVSQ+KTPRCV P N+++LQIL +NVV +Y STA+
Sbjct: 544 ILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

[Close]

BLAST of MC05g0412 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 9.8e-242
Identity = 396/574 (68.99%), Postives = 480/574 (83.62%), Query Frame = 0


Query: 11  EKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFKDCVPLVTHND 70
           E+ I +FE +T+DA RVQ++TL+KILE N SAEYLQ+ GL GRTD +S+K C+PL  HND
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAESYKSCIPLCVHND 66

Query: 71  LEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTMQIYRTSFAFR 130
           +EPYIQRI DG TSP++TG+PI  +SLSSGTT G+PK IPFNDELLETT+QIYRTS+AFR
Sbjct: 67  IEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQIYRTSYAFR 126

Query: 131 NKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQSLCCSPDEVI 190
           N+E+PI  GKALQF+Y SKQ  TKGG+ A TATTN+YR  ++K  MK IQS CCSPDEVI
Sbjct: 127 NREYPIGQGKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQSQCCSPDEVI 186

Query: 191 FGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNIRDGVLSSW 250
           FGPDFHQSLYCHLLCGLI+ +EV  VFSTFAHS+VHAF+TFE VWE+LC++IRDGVLS  
Sbjct: 187 FGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTDIRDGVLSKK 246

Query: 251 VTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYL 310
           VTAP+IR A+SK+L+PNPELAD IY+KC GLSNWYG+IP L+PNAKY+YGIMTGSMEPYL
Sbjct: 247 VTAPSIREAVSKILKPNPELADSIYKKCIGLSNWYGVIPALWPNAKYVYGIMTGSMEPYL 306

Query: 311 KKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEFIPLKESAEEQ 370
           KKLRHYAG+L L+SADYG+SEGWVG+N++P +PPE  T+ VLP +GYFEFIPL++   E+
Sbjct: 307 KKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEE 366

Query: 371 KQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMGFHNATPEVKF 430
            +N   ++   E+ PVGLTEV+VG+ YE+++TN AGLYRYRLGDVVK+  FHN+TPE++F
Sbjct: 367 TEN-SASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDVVKIARFHNSTPELQF 426

Query: 431 VCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSREPGHYVIFWET 490
           +CRR+L+LSINIDKNTEKDLQ+AVE A   L  EKLEV+DFTS V+ S +PG YVIFWE 
Sbjct: 427 ICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWEL 486

Query: 491 SGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAA 550
           SG+A  EVL  C N LD AF+DAGY  SRK+  IG LELR++RKGTF +I+DH+LSLG A
Sbjct: 487 SGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGA 546

Query: 551 VSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
           VSQ+KTPR V P+N+ VLQIL  NV  SYFSTAY
Sbjct: 547 VSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAY 579

[Close]

BLAST of MC05g0412 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 815.1 bits (2104), Expect = 5.2e-235
Identity = 388/587 (66.10%), Postives = 483/587 (82.28%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGR-TDP-QS 60
           ML+K+E FD  +VI++F+EMT++A +VQ++TL++IL +N SA YLQ+ GLNG  TDP ++
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLET 120
           FK  VPLVT  +LEPYI+R+ DG TSPILTG P+  ISLSSGT+QGRPK IPF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TMQIYRTSFAFRNKEFPI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180
           T+Q++RT+FAFRN++FPI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK  MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIQSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240
           +I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE VWEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           + ++I+DGVLS+ +T P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360
           +YGIMTGSMEPY+ KLRHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420
           FEF+P+ E+ E             E KPVGLT+VK+GEEYE+++TN AGLYRYRLGDVVK
Sbjct: 361 FEFLPVSETGE------------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVK 420

Query: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480
           V+GF+N TP++KF+CRRNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S++D+
Sbjct: 421 VIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDV 480

Query: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540
           S +PGHY IFWE SGE   +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV KGTF
Sbjct: 481 STDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
            KI +H+L LG++  Q+K PRCV P+N  VLQILC NVV+SYFSTA+
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of MC05g0412 vs. ExPASy Swiss-Prot
Match: Q53P49 (Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.12 PE=2 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 6.9e-179
Identity = 315/598 (52.68%), Postives = 417/598 (69.73%), Query Frame = 0


Query: 9   DGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFKDCVPLVTH 68
           + E+++  FE  T+DA  VQRETL +IL EN   EYL+ LGL G TD  SF+  VP+VTH
Sbjct: 13  EDEEIMAWFERTTRDAADVQRETLRRILAENAGVEYLRELGLAGLTDAGSFRARVPVVTH 72

Query: 69  NDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTMQIYRTSFA 128
            DL+PYIQR+ADG  SP+LT KP+  ISLSSGTTQG+ K + FND+LL ++++ +  S+A
Sbjct: 73  ADLDPYIQRVADGDASPVLTAKPVTAISLSSGTTQGKRKRLLFNDDLLRSSIRFFHASYA 132

Query: 129 FRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKA---IQSLCCS 188
           F N+ FP+ +G+ LQF+Y S+   TKGGL A T  TN+ RS +F  +M A    +   CS
Sbjct: 133 FTNRAFPVEDGRVLQFMYGSRHETTKGGLTATTVMTNLLRSEEFTASMAARSRPRLPSCS 192

Query: 189 PDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNIRDG 248
           P EV+F PDF +SLYCHLLCGL+   EV  V ++FAHSIV A +  E VW ELC++IR G
Sbjct: 193 PSEVVFSPDFDESLYCHLLCGLLLAGEVRAVSASFAHSIVVALQALERVWRELCADIRRG 252

Query: 249 VLS-SWVTAPTIRAAMSKLL-RPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMT 308
             S + VT P +R A++ +L  PNP LAD + R+CA L +W G+IP L+PNA+Y+   MT
Sbjct: 253 AASPARVTTPAVRRAVAPILAAPNPALADALERRCAALGDWSGVIPALWPNARYVQATMT 312

Query: 309 GSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEFIPL 368
           GSME Y+KKLRHYAG + L+S +Y SSEG +G N     PPE   F VLP+  YFEFIPL
Sbjct: 313 GSMEHYVKKLRHYAGGVPLVSGNYASSEGVIGINAEQHAPPESVVFTVLPDAAYFEFIPL 372

Query: 369 KESAEEQKQNEDGALVAAEN--------KPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDV 428
           K    +   ++D    A  +         PVGLT+V VGE YE+++T   GLYRYRLGDV
Sbjct: 373 KPPCTDAAADDDNPAAAGSSCYVDADDANPVGLTDVVVGEHYEVVMTTFTGLYRYRLGDV 432

Query: 429 VKVMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLA-----EEKLEVVD 488
           VKV GFH+ATP+++FVCRR+L+LSIN+DKN+E DLQ+AV++A  +LA      ++LE+ D
Sbjct: 433 VKVAGFHHATPKLRFVCRRSLILSINVDKNSEHDLQLAVDSAAKILAGDGENHKQLEIAD 492

Query: 489 FTSHVDMSREPGHYVIFWETSG---EAKGEVLGECCNCLDRAF-LDAGYVSSRKVNAIGA 548
           +TSH D S +PGHYV+FWE +G   E  G VL  CC+ +DRAF  DAGY  SRK  AIGA
Sbjct: 493 YTSHADTSSDPGHYVVFWELNGGGEEDGGGVLQRCCDEMDRAFGADAGYAQSRKTCAIGA 552

Query: 549 LELRVVRKGTFHKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
           LELRV+R+G F +++ HY++ G++  Q+K PRCV P+N  VL++L  N +N +FSTAY
Sbjct: 553 LELRVLRRGAFQEVLRHYVAGGSSAGQFKMPRCVAPSNAGVLRVLKDNTINIFFSTAY 610

[Close]

BLAST of MC05g0412 vs. NCBI nr
Match: XP_022147427.1 (jasmonic acid-amido synthetase JAR1-like [Momordica charantia] >XP_022147428.1 jasmonic acid-amido synthetase JAR1-like [Momordica charantia])

HSP 1 Score: 1172 bits (3033), Expect = 0.0
Identity = 584/584 (100.00%), Postives = 584/584 (100.00%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK
Sbjct: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM 120
           DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ 180
           QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ
Sbjct: 121 QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ 180

Query: 181 SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS 240
           SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS
Sbjct: 181 SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS 240

Query: 241 NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF 360
           IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF 360

Query: 361 IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG 420
           IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG
Sbjct: 361 IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG 420

Query: 421 FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE 480
           FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE
Sbjct: 421 FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE 480

Query: 481 PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI 540
           PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI
Sbjct: 481 PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI 540

Query: 541 MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY
Sbjct: 541 MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584

[Close]

BLAST of MC05g0412 vs. NCBI nr
Match: XP_023539820.1 (jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539821.1 jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539822.1 jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1068 bits (2761), Expect = 0.0
Identity = 532/586 (90.78%), Postives = 563/586 (96.08%), Query Frame = 0


Query: 1   MLDKM-EAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+KM EAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120
           KDCVPLV H+DLE YIQRIADGA+SPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT
Sbjct: 61  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120

Query: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAI 180
           MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TMK I
Sbjct: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTMKTI 180

Query: 181 QSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELC 240
           QS CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWEELC
Sbjct: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWEELC 240

Query: 241 SNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IRDGVLSS VTA +IR AMSK+L+PNP+LADLIY+KCAGLSNWYGLIPELFPNA+YIY
Sbjct: 241 GDIRDGVLSSRVTALSIRTAMSKILKPNPDLADLIYQKCAGLSNWYGLIPELFPNAQYIY 300

Query: 301 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360
           GIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE
Sbjct: 301 GIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360

Query: 361 FIPLKESAEEQKQNEDG-ALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKV 420
           FIPLKE++++Q+Q  DG A+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGDVVKV
Sbjct: 361 FIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDVVKV 420

Query: 421 MGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMS 480
           MGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHVD+S
Sbjct: 421 MGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHVDLS 480

Query: 481 REPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFH 540
           REPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KGTFH
Sbjct: 481 REPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKGTFH 540

Query: 541 KIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           KIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVVNSYFSTA+
Sbjct: 541 KIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVNSYFSTAF 586

[Close]

BLAST of MC05g0412 vs. NCBI nr
Match: XP_022974649.1 (jasmonic acid-amido synthetase JAR1 [Cucurbita maxima] >XP_022974650.1 jasmonic acid-amido synthetase JAR1 [Cucurbita maxima] >XP_022974651.1 jasmonic acid-amido synthetase JAR1 [Cucurbita maxima])

HSP 1 Score: 1066 bits (2757), Expect = 0.0
Identity = 532/586 (90.78%), Postives = 561/586 (95.73%), Query Frame = 0


Query: 1   MLDKM-EAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+KM EAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMVEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120
           KDCVPLV H DLE YIQRIADGA+SPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT
Sbjct: 61  KDCVPLVYHEDLESYIQRIADGASSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120

Query: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAI 180
           MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TMK I
Sbjct: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTMKTI 180

Query: 181 QSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELC 240
           QS CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWEELC
Sbjct: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWEELC 240

Query: 241 SNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+YIY
Sbjct: 241 GDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQYIY 300

Query: 301 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360
           GIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE
Sbjct: 301 GIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360

Query: 361 FIPLKESAEEQKQNEDG-ALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKV 420
           FIPLKE++++Q+Q  DG A+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGDVVKV
Sbjct: 361 FIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDVVKV 420

Query: 421 MGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMS 480
           MGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHVD+S
Sbjct: 421 MGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHVDLS 480

Query: 481 REPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFH 540
           REPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KGTFH
Sbjct: 481 REPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKGTFH 540

Query: 541 KIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           KIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFSTA+
Sbjct: 541 KIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFSTAF 586

[Close]

BLAST of MC05g0412 vs. NCBI nr
Match: KAG6597096.1 (Jasmonoyl--L-amino acid synthetase JAR6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1065 bits (2755), Expect = 0.0
Identity = 531/586 (90.61%), Postives = 562/586 (95.90%), Query Frame = 0


Query: 1   MLDKM-EAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+KM EAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 29  MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 88

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120
           KDCVPLV H+DLE YIQRIADGA+SPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT
Sbjct: 89  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 148

Query: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAI 180
           MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TMK I
Sbjct: 149 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTMKTI 208

Query: 181 QSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELC 240
           QS CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWEELC
Sbjct: 209 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWEELC 268

Query: 241 SNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+YIY
Sbjct: 269 GDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQYIY 328

Query: 301 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360
           GIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE
Sbjct: 329 GIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 388

Query: 361 FIPLKESAEEQKQNEDG-ALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKV 420
           FIPLKE++++Q+Q  DG A+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGD+VKV
Sbjct: 389 FIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDLVKV 448

Query: 421 MGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMS 480
           MGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHVD+S
Sbjct: 449 MGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHVDLS 508

Query: 481 REPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFH 540
           REPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KGTFH
Sbjct: 509 REPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKGTFH 568

Query: 541 KIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           KIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFSTA+
Sbjct: 569 KIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFSTAF 614

[Close]

BLAST of MC05g0412 vs. NCBI nr
Match: XP_022941396.1 (jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941403.1 jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941412.1 jasmonic acid-amido synthetase JAR1 [Cucurbita moschata])

HSP 1 Score: 1065 bits (2755), Expect = 0.0
Identity = 531/586 (90.61%), Postives = 562/586 (95.90%), Query Frame = 0


Query: 1   MLDKM-EAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+KM EAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120
           KDCVPLV H+DLE YIQRIADGA+SPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT
Sbjct: 61  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120

Query: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAI 180
           MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TMK I
Sbjct: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTMKTI 180

Query: 181 QSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELC 240
           QS CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWEELC
Sbjct: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWEELC 240

Query: 241 SNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+YIY
Sbjct: 241 GDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQYIY 300

Query: 301 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360
           GIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE
Sbjct: 301 GIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360

Query: 361 FIPLKESAEEQKQNEDG-ALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKV 420
           FIPLKE++++Q+Q  DG A+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGD+VKV
Sbjct: 361 FIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDLVKV 420

Query: 421 MGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMS 480
           MGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHVD+S
Sbjct: 421 MGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHVDLS 480

Query: 481 REPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFH 540
           REPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KGTFH
Sbjct: 481 REPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKGTFH 540

Query: 541 KIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           KIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFSTA+
Sbjct: 541 KIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFSTAF 586

[Close]

BLAST of MC05g0412 vs. ExPASy TrEMBL
Match: A0A6J1D2B9 (jasmonic acid-amido synthetase JAR1-like OS=Momordica charantia OX=3673 GN=LOC111016355 PE=3 SV=1)

HSP 1 Score: 1172 bits (3033), Expect = 0.0
Identity = 584/584 (100.00%), Postives = 584/584 (100.00%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK
Sbjct: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM 120
           DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ 180
           QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ
Sbjct: 121 QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ 180

Query: 181 SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS 240
           SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS
Sbjct: 181 SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS 240

Query: 241 NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF 360
           IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF 360

Query: 361 IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG 420
           IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG
Sbjct: 361 IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG 420

Query: 421 FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE 480
           FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE
Sbjct: 421 FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE 480

Query: 481 PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI 540
           PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI
Sbjct: 481 PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI 540

Query: 541 MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY
Sbjct: 541 MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584

[Close]

BLAST of MC05g0412 vs. ExPASy TrEMBL
Match: A0A6J1II84 (jasmonic acid-amido synthetase JAR1 OS=Cucurbita maxima OX=3661 GN=LOC111473333 PE=3 SV=1)

HSP 1 Score: 1066 bits (2757), Expect = 0.0
Identity = 532/586 (90.78%), Postives = 561/586 (95.73%), Query Frame = 0


Query: 1   MLDKM-EAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+KM EAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMVEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120
           KDCVPLV H DLE YIQRIADGA+SPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT
Sbjct: 61  KDCVPLVYHEDLESYIQRIADGASSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120

Query: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAI 180
           MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TMK I
Sbjct: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTMKTI 180

Query: 181 QSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELC 240
           QS CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWEELC
Sbjct: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWEELC 240

Query: 241 SNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+YIY
Sbjct: 241 GDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQYIY 300

Query: 301 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360
           GIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE
Sbjct: 301 GIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360

Query: 361 FIPLKESAEEQKQNEDG-ALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKV 420
           FIPLKE++++Q+Q  DG A+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGDVVKV
Sbjct: 361 FIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDVVKV 420

Query: 421 MGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMS 480
           MGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHVD+S
Sbjct: 421 MGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHVDLS 480

Query: 481 REPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFH 540
           REPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KGTFH
Sbjct: 481 REPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKGTFH 540

Query: 541 KIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           KIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFSTA+
Sbjct: 541 KIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFSTAF 586

[Close]

BLAST of MC05g0412 vs. ExPASy TrEMBL
Match: A0A6J1FL00 (jasmonic acid-amido synthetase JAR1 OS=Cucurbita moschata OX=3662 GN=LOC111446696 PE=3 SV=1)

HSP 1 Score: 1065 bits (2755), Expect = 0.0
Identity = 531/586 (90.61%), Postives = 562/586 (95.90%), Query Frame = 0


Query: 1   MLDKM-EAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSF 60
           ML+KM EAFDGEKVIEQFEEMT+DAERVQ+ETL+KILEENGSAEYLQ+LGLNGRTDPQSF
Sbjct: 1   MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120
           KDCVPLV H+DLE YIQRIADGA+SPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT
Sbjct: 61  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120

Query: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAI 180
           MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFK TMK I
Sbjct: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTMKTI 180

Query: 181 QSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELC 240
           QS CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVHAFRTFELVWEELC
Sbjct: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWEELC 240

Query: 241 SNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IRDGVLSS VTA +IR AMSK+L+PNPELADLIY+KCAGLSNWYGLIPELFPNA+YIY
Sbjct: 241 GDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQYIY 300

Query: 301 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360
           GIMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE
Sbjct: 301 GIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360

Query: 361 FIPLKESAEEQKQNEDG-ALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKV 420
           FIPLKE++++Q+Q  DG A+VA ENKPVGLTEVKVGE YEIIVTNVAGLYRYRLGD+VKV
Sbjct: 361 FIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDLVKV 420

Query: 421 MGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMS 480
           MGFHNATPE+KF+CR NLLLSINIDKNTEKDLQ+AVEAAG++LA EKLEVV+FTSHVD+S
Sbjct: 421 MGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHVDLS 480

Query: 481 REPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFH 540
           REPGHYVIFWE SGEAKGEVLGECCNCLDRAFLDAGY+SSRKVNAIGALELRVV KGTFH
Sbjct: 481 REPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKGTFH 540

Query: 541 KIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           KIM+HYLSLGAAVSQYKTPRCV P NTTVLQILCANVV SYFSTA+
Sbjct: 541 KIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFSTAF 586

[Close]

BLAST of MC05g0412 vs. ExPASy TrEMBL
Match: A0A1S4DTP6 (jasmonic acid-amido synthetase JAR1 OS=Cucumis melo OX=3656 GN=LOC103483208 PE=3 SV=1)

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 524/584 (89.73%), Postives = 553/584 (94.69%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           ML+KMEAFDGEKVIEQFEEMT+DAERVQRETL+KILEENGSAEYLQ+LGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM 120
           DCVPLV+H+DLE YIQRIADG +SPILTGKPIKTISLSSGTT+GRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVSHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ 180
           QIYRTSFAFRNKE P+  GKALQFIYSSKQFKT GGLAAGTATTNVYRSA+FK TM+AIQ
Sbjct: 121 QIYRTSFAFRNKEVPLGKGKALQFIYSSKQFKTNGGLAAGTATTNVYRSAQFKSTMRAIQ 180

Query: 181 SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS 240
           S CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVH+FRTFE VWEELCS
Sbjct: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240

Query: 241 NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVTAP+IRAAMSKLL+PNPELADLIYRKC GLSNWYGLIPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTAPSIRAAMSKLLKPNPELADLIYRKCEGLSNWYGLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF 360
           IMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFAVLPNIGYFEF 360

Query: 361 IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG 420
           IPLKE+A+ Q Q          NKP+GLTEVK+GEEYEIIVTNVAGLYRYRLGD VKVMG
Sbjct: 361 IPLKENAQGQHQR---------NKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDAVKVMG 420

Query: 421 FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE 480
           FHN+TPE+KF+CRRNLLLSINIDK TEKDLQ+AVEAAG++LA EKLEVVDFTS+VD+SRE
Sbjct: 421 FHNSTPELKFICRRNLLLSINIDKITEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDVSRE 480

Query: 481 PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI 540
           PGHYVIFWE SGEAKGEVLGEC NCLDRAFLDAGY+SSRKVNAIGALELRVVRKGTFHKI
Sbjct: 481 PGHYVIFWEISGEAKGEVLGECSNCLDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKI 540

Query: 541 MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           MDH+LSLGAAVSQYKTPRCVIPTNT VLQILC+NVVNSYFSTAY
Sbjct: 541 MDHHLSLGAAVSQYKTPRCVIPTNTAVLQILCSNVVNSYFSTAY 575

[Close]

BLAST of MC05g0412 vs. ExPASy TrEMBL
Match: E5GCH6 (Auxin-regulated protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 524/584 (89.73%), Postives = 553/584 (94.69%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60
           ML+KMEAFDGEKVIEQFEEMT+DAERVQRETL+KILEENGSAEYLQ+LGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM 120
           DCVPLV+H+DLE YIQRIADG +SPILTGKPIKTISLSSGTT+GRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVSHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ 180
           QIYRTSFAFRNKE P+  GKALQFIYSSKQFKT GGLAAGTATTNVYRSA+FK TM+AIQ
Sbjct: 121 QIYRTSFAFRNKEVPLGKGKALQFIYSSKQFKTNGGLAAGTATTNVYRSAQFKSTMRAIQ 180

Query: 181 SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS 240
           S CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVH+FRTFE VWEELCS
Sbjct: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240

Query: 241 NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVTAP+IRAAMSKLL+PNPELADLIYRKC GLSNWYGLIPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTAPSIRAAMSKLLKPNPELADLIYRKCEGLSNWYGLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF 360
           IMTGSMEPYLKKLRHYAG L LMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFAVLPNIGYFEF 360

Query: 361 IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG 420
           IPLKE+A+ Q Q          NKP+GLTEVK+GEEYEIIVTNVAGLYRYRLGD VKVMG
Sbjct: 361 IPLKENAQGQHQR---------NKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDAVKVMG 420

Query: 421 FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE 480
           FHN+TPE+KF+CRRNLLLSINIDK TEKDLQ+AVEAAG++LA EKLEVVDFTS+VD+SRE
Sbjct: 421 FHNSTPELKFICRRNLLLSINIDKITEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDVSRE 480

Query: 481 PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI 540
           PGHYVIFWE SGEAKGEVLGEC NCLDRAFLDAGY+SSRKVNAIGALELRVVRKGTFHKI
Sbjct: 481 PGHYVIFWEISGEAKGEVLGECSNCLDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKI 540

Query: 541 MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584
           MDH+LSLGAAVSQYKTPRCVIPTNT VLQILC+NVVNSYFSTAY
Sbjct: 541 MDHHLSLGAAVSQYKTPRCVIPTNTAVLQILCSNVVNSYFSTAY 575

[Close]

BLAST of MC05g0412 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 815.1 bits (2104), Expect = 3.7e-236
Identity = 388/587 (66.10%), Postives = 483/587 (82.28%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGR-TDP-QS 60
           ML+K+E FD  +VI++F+EMT++A +VQ++TL++IL +N SA YLQ+ GLNG  TDP ++
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLET 120
           FK  VPLVT  +LEPYI+R+ DG TSPILTG P+  ISLSSGT+QGRPK IPF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TMQIYRTSFAFRNKEFPI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180
           T+Q++RT+FAFRN++FPI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK  MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIQSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240
           +I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE VWEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           + ++I+DGVLS+ +T P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360
           +YGIMTGSMEPY+ KLRHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420
           FEF+P+ E+ E             E KPVGLT+VK+GEEYE+++TN AGLYRYRLGDVVK
Sbjct: 361 FEFLPVSETGE------------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVK 420

Query: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480
           V+GF+N TP++KF+CRRNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S++D+
Sbjct: 421 VIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDV 480

Query: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540
           S +PGHY IFWE SGE   +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV KGTF
Sbjct: 481 STDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
            KI +H+L LG++  Q+K PRCV P+N  VLQILC NVV+SYFSTA+
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of MC05g0412 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 815.1 bits (2104), Expect = 3.7e-236
Identity = 388/587 (66.10%), Postives = 483/587 (82.28%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGR-TDP-QS 60
           ML+K+E FD  +VI++F+EMT++A +VQ++TL++IL +N SA YLQ+ GLNG  TDP ++
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLET 120
           FK  VPLVT  +LEPYI+R+ DG TSPILTG P+  ISLSSGT+QGRPK IPF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TMQIYRTSFAFRNKEFPI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180
           T+Q++RT+FAFRN++FPI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK  MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIQSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240
           +I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE VWEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           + ++I+DGVLS+ +T P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360
           +YGIMTGSMEPY+ KLRHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420
           FEF+P+ E+ E             E KPVGLT+VK+GEEYE+++TN AGLYRYRLGDVVK
Sbjct: 361 FEFLPVSETGE------------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVK 420

Query: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480
           V+GF+N TP++KF+CRRNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S++D+
Sbjct: 421 VIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDV 480

Query: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540
           S +PGHY IFWE SGE   +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV KGTF
Sbjct: 481 STDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 540

Query: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
            KI +H+L LG++  Q+K PRCV P+N  VLQILC NVV+SYFSTA+
Sbjct: 541 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of MC05g0412 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 815.1 bits (2104), Expect = 3.7e-236
Identity = 388/587 (66.10%), Postives = 483/587 (82.28%), Query Frame = 0


Query: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGR-TDP-QS 60
           ML+K+E FD  +VI++F+EMT++A +VQ++TL++IL +N SA YLQ+ GLNG  TDP ++
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 71

Query: 61  FKDCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLET 120
           FK  VPLVT  +LEPYI+R+ DG TSPILTG P+  ISLSSGT+QGRPK IPF DEL+E 
Sbjct: 72  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 131

Query: 121 TMQIYRTSFAFRNKEFPI-RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMK 180
           T+Q++RT+FAFRN++FPI  NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK  MK
Sbjct: 132 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 191

Query: 181 AIQSLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEE 240
           +I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE VWEE
Sbjct: 192 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 251

Query: 241 LCSNIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           + ++I+DGVLS+ +T P++R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPNAKY
Sbjct: 252 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 311

Query: 301 IYGIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGY 360
           +YGIMTGSMEPY+ KLRHYAGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN+GY
Sbjct: 312 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 371

Query: 361 FEFIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVK 420
           FEF+P+ E+ E             E KPVGLT+VK+GEEYE+++TN AGLYRYRLGDVVK
Sbjct: 372 FEFLPVSETGE------------GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVK 431

Query: 421 VMGFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDM 480
           V+GF+N TP++KF+CRRNL+LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S++D+
Sbjct: 432 VIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDV 491

Query: 481 SREPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTF 540
           S +PGHY IFWE SGE   +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV KGTF
Sbjct: 492 STDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTF 551

Query: 541 HKIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
            KI +H+L LG++  Q+K PRCV P+N  VLQILC NVV+SYFSTA+
Sbjct: 552 RKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

[Close]

BLAST of MC05g0412 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 735.3 bits (1897), Expect = 3.7e-212
Identity = 343/508 (67.52%), Postives = 419/508 (82.48%), Query Frame = 0


Query: 78  IADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTMQIYRTSFAFRNKEFPI- 137
           + DG TSPILTG P+  ISLSSGT+QGRPK IPF DEL+E T+Q++RT+FAFRN++FPI 
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 138 RNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQSLCCSPDEVIFGPDFH 197
            NGKALQFI+SSKQ+ + GG+  GTATTNVYR+  FK  MK+I S  CSPDEVIF PD H
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 198 QSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNIRDGVLSSWVTAPTI 257
           Q+LYCHLL G++FRD+V++VF+ FAH +VHAFRTFE VWEE+ ++I+DGVLS+ +T P++
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 258 RAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYLKKLRHY 317
           R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPNAKY+YGIMTGSMEPY+ KLRHY
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHY 240

Query: 318 AGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEFIPLKESAEEQKQNEDG 377
           AGDL L+S DYGSSEGW+ ANV P L PE ATF V+PN+GYFEF+P+ E+ E        
Sbjct: 241 AGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPVSETGE-------- 300

Query: 378 ALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMGFHNATPEVKFVCRRNL 437
                E KPVGLT+VK+GEEYE+++TN AGLYRYRLGDVVKV+GF+N TP++KF+CRRNL
Sbjct: 301 ----GEEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNL 360

Query: 438 LLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSREPGHYVIFWETSGEAKG 497
           +LSINIDKNTE+DLQ++VE+A   L+EEK+EV+DF+S++D+S +PGHY IFWE SGE   
Sbjct: 361 ILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYAIFWEISGETNE 420

Query: 498 EVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAAVSQYKT 557
           +VL +CCNCLDRAF+DAGYVSSRK   IGALELRVV KGTF KI +H+L LG++  Q+K 
Sbjct: 421 DVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKM 480

Query: 558 PRCVIPTNTTVLQILCANVVNSYFSTAY 585
           PRCV P+N  VLQILC NVV+SYFSTA+
Sbjct: 481 PRCVKPSNAKVLQILCENVVSSYFSTAF 496

[Close]

BLAST of MC05g0412 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 589.7 bits (1519), Expect = 2.5e-168
Identity = 305/586 (52.05%), Postives = 402/586 (68.60%), Query Frame = 0


Query: 11  EKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQS-LG------LNGRTDPQSFKDCV 70
           + VI  FE ++++A +VQ ETL +ILE N   EYL+  LG      ++  T    F   V
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKMDDYTLETLFTSLV 68

Query: 71  PLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTMQIY 130
           P+V+H DL+PYIQRIADG TSP+LT +PI  +SLSSGTT+GR K +PF     +TT+QI+
Sbjct: 69  PIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQIF 128

Query: 131 RTSFAFRNKEFPIR-NGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQSL 190
           R S A+R++ +PIR  G+ L+FIY+ K+FKT GGL  GTATT+ Y S +FK   +  +S 
Sbjct: 129 RLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKSF 188

Query: 191 CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCSNI 250
            CSP EVI G DF Q  YCHLL GL +  +VEFV S F+++IV AF  FE +W E+C++I
Sbjct: 189 TCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICADI 248

Query: 251 RDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLS---NWYGLIPELFPNAKYIY 310
           ++G LSS +T P +R A+  L+RPNP LA  I   C  L     W+GLI +L+PNAK+I 
Sbjct: 249 KEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFIS 308

Query: 311 GIMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 370
            IMTGSM PYL KLRHYAG L L+SADYGS+E W+G NV+P LPPE  +F V+P   YFE
Sbjct: 309 SIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYFE 368

Query: 371 FIPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVM 430
           FIPL     +     DG  V  E+KPV L++VK+G+EYE+++T   GLYRYRLGDVV+V 
Sbjct: 369 FIPLYRRQNQSDICIDGDFV--EDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGDVVEVT 428

Query: 431 GFHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEE-KLEVVDFTSHVDMS 490
            FH  TP++ F+ RR L+L+INIDKNTEKDLQ  V+ A  LL+   + EVVDFTSH D+ 
Sbjct: 429 SFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTSHADVI 488

Query: 491 REPGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFH 550
             PGHYVI+WE  GEA  + L ECC  +D AF+D GYV SR++N+IG LELRVV +GTF 
Sbjct: 489 ARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVVERGTFG 548

Query: 551 KIMDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 585
           K+ +  +     ++Q+KTPRC   TN+ +L IL  + +  + S+AY
Sbjct: 549 KVAERCVGKCGGLNQFKTPRCT--TNSVMLDILNDSTIKRFRSSAY 590

The following BLAST results are available for this feature:

BLAST of MC05g0412 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1J6KGJ9	2.6e-266	75.51	Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ...	[more]
A0A314KSQ4	7.4e-266	75.90	Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ...	[more]
Q6I581	9.8e-242	68.99	Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9SKE2	5.2e-235	66.10	Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ...	[more]
Q53P49	6.9e-179	52.68	Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. jap...	[more]

BLAST of MC05g0412 vs. NCBI nr


Match Name	Identity	Description
XP_022147427.1	100.00	jasmonic acid-amido synthetase JAR1-like [Momordica charantia] >XP_022147428.1 j...	[more]
XP_023539820.1	90.78	jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539821.1...	[more]
XP_022974649.1	90.78	jasmonic acid-amido synthetase JAR1 [Cucurbita maxima] >XP_022974650.1 jasmonic ...	[more]
KAG6597096.1	90.61	Jasmonoyl--L-amino acid synthetase JAR6, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022941396.1	90.61	jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941403.1 jasmoni...	[more]

BLAST of MC05g0412 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1D2B9	100.00	jasmonic acid-amido synthetase JAR1-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1II84	90.78	jasmonic acid-amido synthetase JAR1 OS=Cucurbita maxima OX=3661 GN=LOC111473333 ...	[more]
A0A6J1FL00	90.61	jasmonic acid-amido synthetase JAR1 OS=Cucurbita moschata OX=3662 GN=LOC11144669...	[more]
A0A1S4DTP6	89.73	jasmonic acid-amido synthetase JAR1 OS=Cucumis melo OX=3656 GN=LOC103483208 PE=3...	[more]
E5GCH6	89.73	Auxin-regulated protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]

BLAST of MC05g0412 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46370.1	3.7e-236	66.10	Auxin-responsive GH3 family protein	[more]
AT2G46370.2	3.7e-236	66.10	Auxin-responsive GH3 family protein	[more]
AT2G46370.4	3.7e-236	66.10	Auxin-responsive GH3 family protein	[more]
AT2G46370.3	3.7e-212	67.52	Auxin-responsive GH3 family protein	[more]
AT4G03400.1	2.5e-168	52.05	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 364..384
None	No IPR available	PANTHER	PTHR31901:SF40	JASMONIC ACID-AMIDO SYNTHETASE JAR1-LIKE ISOFORM X1	coord: 7..570
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 16..560 e-value: 2.5E-192 score: 640.4
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 7..570

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0412.1	MC05g0412.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016881	acid-amino acid ligase activity
molecular_function	GO:0102053	(-)-jasmonoyl-isoleucine synthetase activity
molecular_function	GO:0102058	jasmonoyl-leucine synthetase activity
molecular_function	GO:0102057	jasmonoyl-valine synthetase activity