MC05g0147 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0147
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	4-methyl-5-beta-hydroxyethylthiazole kinase
Location	MC05: 1021838 .. 1024781 (-)
RNA-Seq Expression	MC05g0147
Synteny	MC05g0147

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATGAATTTTTCATTTATTGAAAAAAAATATATTGAAATTGAAACGAAGGCGACAAAATCCAATTCAAGACGAGAGAGAGGAGAATCTACGGGCGATGAATTCGAAGGAAAACGAAGACGAAGCGCAGCGACGGCGGTGGGGAGCAAGAGCGTGGGCGACACTCGCCGCCGTGCGAAGCCGTTCGCCGCTCATCCAATGCATCACGAACTTCGTTTCAATGGATTTGATGGCCAATACTCTCCTCTCCGCCGGTGCATCGCCGGCCATGCTTCATTCAGTCGAGGAGTTGCCGGACTTCACTCCCAACGCCGATGCCCTCTGTATTAATGTCGGCACGCTTTCCGCTGCCTGGTTGCCAGCCATGAAGGTCGCCGCTGAACTGGCGTCCAAGGCCGGGAAGCCCTGGGTTCTCGATCCGGTGGCGGCCGGAGCTTCTAAGTTCCGGTTGATGGCTTGTCTGGAGCTTTTGAATCTGAAGCCCACCGTTGTTAGAGGGAATGGGTCTGAAATAATCGCGTTGTCTAAAGCTTCTACTGAGCCAAGTATGGTAAGATGCAATTTTATACTGCAAATGATATCATTATGAACGATTGCTTCCTCTTCTTTTCTTTCGAACTTCTTCATGCAAAGTTGGAAGCTAGAAGTTCAATCTTAGGGTTTGTTTGTTCTATTGGTGTAAGATATAGTTTTCGTTTTAATATGCCTAAGAGTGTCTGAACTTTATCTTACGAAAAGTTGAGCTTGGGAATCCCAATGGATGAATTCAAGATATATGCTCGAGTTACAATGGAAGTAAAAAACAGGGTCCCCTTTCATAATTGGGGTATCGTAGCCCTAATCCTTCATGAAATCGAGAATCTAAGTGACAAATCAGTTCAATAATGTAGAACCTTAGCTGTAGAAGTTCATCGTTACTATTACTTAGCCTAAACTTTATTACAATTTTAAGGGCGTAGACAGCTGCCATCAGTCAATGGATGCTGTAGAAGCTGCTAAATCATTAGCTCAAACCAGTGGAGCCATAGTAGCAGTATCGGGAGCCGTGGACATAGTTACAGACGGGGAACAAGTTATTGGCGCTCATAATGGAGTGGCCATGATGCAAAAGATTACGGCAACAGGATGTTCTGTAACAGCCCTTATTGCTGCCTTCCTTGCCGTTGATCAACTTCATGCTTTGGAAGCAACAGCTTCAGCATTGTCAATATTTGGAATTGCAGGCGAGATGGGAATGGATTTGGCAAAGGGCCCTGCCTCTCTACGAACTCATTTGATTGATTCACTACATGAGCTCGACGAAGCAGCTATAATCTCGCAAGTTAAGATCACCAGCTTGTGATTAAATTTTGGAGGCGTTTTTATGTATGGTATGTTCAATAACTACTTTTAAACCTTCTTGAGTTTGTACAGTGCTTTACTGTCTAATAATGTTGAAAAGGTTGGCTAGCCTAAACTTCATTTGTTTGTTCATTCGTATTTTATACTTTTCGATGAAGTTTATAGCTCTTGTTAACCTTGTTATTTTGTTTCCTTCTCTTGATATCTTCTTTTTACTGTGTTTATGACCTTGTATGTTTTATGTATATCATGAGGCCGTCTCATTAATCACATTGGACGAGTGGAGTGAATTCCCTTTTAAATTTTATCATCAAATCCAACATAGTTCACTCTTGGTATGAGCACAACAGATTTACAACAGAGAAATATATAAACTATAAAACTCGGTTACATTTCATAGACGTTACAAACCATGATTGCATTTATCACCACACATATATTGCATTGTTCTTTGTTCACCATACTGGTTTTCAGTAGCTTAGATGTACAGCGATCCTCAGCAATGTCTCTGCTCCATCTGTGACGTGCTGGCCCGGAGGAATGAGTGACCGCACCTCTGCAATGCCACGTGCGTGCTTGAACTTGCCAGTTCCTCCAGTCACTGACAAATGTGATGAGGGGCTACCGATCCTGTATACTCCGTAGAAGTTGAGGCTGTCACCATATTCTCCTCCTTCAACCATGGCTGTAAATGCCATCATCTGCATAGTCCCATCAGCTGAACTAGCTACATACACCCCTTGTGCCTTGCCAATCGATTGTGAGCCCAACTCCGGGGCGGTGGTCAAGATGTCATCAATGACAGTAATTGTTCCAAAGCCTAGTCCCAACCCATCAGGTCCCAGTTGGGTCTGAGGGGCATTTTGTAAGTTGGGATTTCCAGCAAAAGCGGTGCCAGACAAGCCAGTCCCTAGAGGGATCCCATTGATGCCATTAACAGTAGGGAGAGCACCATTGGCATTTGGAATTGCTACACCACCGTCTGGGGGGAGGAAACCTATAGGTGTTGCAAAGGGTACTTGACCAGTGTATATGTTCCCCAGCAGGCCAGTAATCGGCCTGGCGGTTGGGCTGCCACCGCCGAGAATGTCGTGCATGTAAAATTCAAGGATCGGCTCATGCCCCCCAGCATTGGCAGTGACAGCTGCAGACTTAGACGCCATAAAATGCACCACGAAGATTGCCATTCCTGCAAGGAAGACGGGAAAAGACGGTTGCCTGAACATGTTGCAAAGTAGGATGAAGAACTTCTCTTGCCTGTAGAACTCTAGAGGATTGTAATTCTTGTGGTGATATGCAAATGGGTATGAACTCTACTCTTTATACAGTGCTGAAAATATTAGATCACTTTGGATTTTGGAGTGGGTATGAACCACCAAAAAAATGGTGAAGTTGTTGATTCTGTTCTTGATGAAATGCATGCTTTCTTCCATCAGATCAAATTGACTAGATTTTCTTGTCACTAATTGGAGCCATAATAGCAGGTCCACTCAAGGAATTAGCTCTGGCCATTATTTTCAGAAGTTTCATAGTTGAGTAGGTAGACTACACTGTGTGAAAATAGTTGTTTTGTTGTGCAGTGTGGAGGGTATAACCAAACCAAA

mRNA sequence

CCATGAATTTTTCATTTATTGAAAAAAAATATATTGAAATTGAAACGAAGGCGACAAAATCCAATTCAAGACGAGAGAGAGGAGAATCTACGGGCGATGAATTCGAAGGAAAACGAAGACGAAGCGCAGCGACGGCGGTGGGGAGCAAGAGCGTGGGCGACACTCGCCGCCGTGCGAAGCCGTTCGCCGCTCATCCAATGCATCACGAACTTCGTTTCAATGGATTTGATGGCCAATACTCTCCTCTCCGCCGGTGCATCGCCGGCCATGCTTCATTCAGTCGAGGAGTTGCCGGACTTCACTCCCAACGCCGATGCCCTCTGTATTAATGTCGGCACGCTTTCCGCTGCCTGGTTGCCAGCCATGAAGGTCGCCGCTGAACTGGCGTCCAAGGCCGGGAAGCCCTGGGTTCTCGATCCGGTGGCGGCCGGAGCTTCTAAGTTCCGGTTGATGGCTTGTCTGGAGCTTTTGAATCTGAAGCCCACCGTTGTTAGAGGGAATGGGTCTGAAATAATCGCGTTGTCTAAAGCTTCTACTGAGCCAAGTATGGGCGTAGACAGCTGCCATCAGTCAATGGATGCTGTAGAAGCTGCTAAATCATTAGCTCAAACCAGTGGAGCCATAGTAGCAGTATCGGGAGCCGTGGACATAGTTACAGACGGGGAACAAGTTATTGGCGCTCATAATGGAGTGGCCATGATGCAAAAGATTACGGCAACAGGATGTTCTGTAACAGCCCTTATTGCTGCCTTCCTTGCCGTTGATCAACTTCATGCTTTGGAAGCAACAGCTTCAGCATTGTCAATATTTGGAATTGCAGGCGAGATGGGAATGGATTTGGCAAAGGGCCCTGCCTCTCTACGAACTCATTTGATTGATTCACTACATGAGCTCGACGAAGCAGCTATAATCTCGCAAGTTAAGATCACCAGCTTGTGATTAAATTTTGGAGGCGTTTTTATGTATGCAATGTCTCTGCTCCATCTGTGACGTGCTGGCCCGGAGGAATGAGTGACCGCACCTCTGCAATGCCACGTGCGTGCTTGAACTTGCCAGTTCCTCCAGTCACTGACAAATGTGATGAGGGGCTACCGATCCTGTATACTCCGTAGAAGTTGAGGCTGTCACCATATTCTCCTCCTTCAACCATGGCTGTAAATGCCATCATCTGCATAGTCCCATCAGCTGAACTAGCTACATACACCCCTTGTGCCTTGCCAATCGATTGTGAGCCCAACTCCGGGGCGGTGGTCAAGATGTCATCAATGACAGTAATTGTTCCAAAGCCTAGTCCCAACCCATCAGGTCCCAGTTGGGTCTGAGGGGCATTTTGTAAGTTGGGATTTCCAGCAAAAGCGGTGCCAGACAAGCCAGTCCCTAGAGGGATCCCATTGATGCCATTAACAGTAGGGAGAGCACCATTGGCATTTGGAATTGCTACACCACCGTCTGGGGGGAGGAAACCTATAGGTGTTGCAAAGGGTACTTGACCAGTGTATATGTTCCCCAGCAGGCCAGTAATCGGCCTGGCGGTTGGGCTGCCACCGCCGAGAATGTCGTGCATGTAAAATTCAAGGATCGGCTCATGCCCCCCAGCATTGGCAGTGACAGCTGCAGACTTAGACGCCATAAAATGCACCACGAAGATTGCCATTCCTGCAAGGAAGACGGGAAAAGACGGTTGCCTGAACATGTTGCAAAGTAGGATGAAGAACTTCTCTTGCCTGTAGAACTCTAGAGGATTGTAATTCTTGTGGTGATATGCAAATGGGTATGAACTCTACTCTTTATACAGTGCTGAAAATATTAGATCACTTTGGATTTTGGAGTGGGTATGAACCACCAAAAAAATGGTGAAGTTGTTGATTCTGTTCTTGATGAAATGCATGCTTTCTTCCATCAGATCAAATTGACTAGATTTTCTTGTCACTAATTGGAGCCATAATAGCAGGTCCACTCAAGGAATTAGCTCTGGCCATTATTTTCAGAAGTTTCATAGTTGAGTAGGTAGACTACACTGTGTGAAAATAGTTGTTTTGTTGTGCAGTGTGGAGGGTATAACCAAACCAAA

Coding sequence (CDS)

ATGAATTCGAAGGAAAACGAAGACGAAGCGCAGCGACGGCGGTGGGGAGCAAGAGCGTGGGCGACACTCGCCGCCGTGCGAAGCCGTTCGCCGCTCATCCAATGCATCACGAACTTCGTTTCAATGGATTTGATGGCCAATACTCTCCTCTCCGCCGGTGCATCGCCGGCCATGCTTCATTCAGTCGAGGAGTTGCCGGACTTCACTCCCAACGCCGATGCCCTCTGTATTAATGTCGGCACGCTTTCCGCTGCCTGGTTGCCAGCCATGAAGGTCGCCGCTGAACTGGCGTCCAAGGCCGGGAAGCCCTGGGTTCTCGATCCGGTGGCGGCCGGAGCTTCTAAGTTCCGGTTGATGGCTTGTCTGGAGCTTTTGAATCTGAAGCCCACCGTTGTTAGAGGGAATGGGTCTGAAATAATCGCGTTGTCTAAAGCTTCTACTGAGCCAAGTATGGGCGTAGACAGCTGCCATCAGTCAATGGATGCTGTAGAAGCTGCTAAATCATTAGCTCAAACCAGTGGAGCCATAGTAGCAGTATCGGGAGCCGTGGACATAGTTACAGACGGGGAACAAGTTATTGGCGCTCATAATGGAGTGGCCATGATGCAAAAGATTACGGCAACAGGATGTTCTGTAACAGCCCTTATTGCTGCCTTCCTTGCCGTTGATCAACTTCATGCTTTGGAAGCAACAGCTTCAGCATTGTCAATATTTGGAATTGCAGGCGAGATGGGAATGGATTTGGCAAAGGGCCCTGCCTCTCTACGAACTCATTTGATTGATTCACTACATGAGCTCGACGAAGCAGCTATAATCTCGCAAGTTAAGATCACCAGCTTGTGA

Protein sequence

MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMACLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVSGAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL

Homology

[Close]

BLAST of MC05g0147 vs. ExPASy Swiss-Prot
Match: Q9LIQ4 (Hydroxyethylthiazole kinase OS=Arabidopsis thaliana OX=3702 GN=THIM PE=1 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.4e-97
Identity = 182/274 (66.42%), Postives = 219/274 (79.93%), Query Frame = 0


Query: 7   EDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELP 66
           E ++++  W +  WA L AVR +SPL+QCITNFVSMDL+ANTLLSAGASPAM+HSV E+P
Sbjct: 2   ESKSEQNEWSSGVWAHLTAVRQQSPLVQCITNFVSMDLVANTLLSAGASPAMVHSVVEIP 61

Query: 67  DFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMACLELLN 126
           DFTP+  ALC+NVGTL+  WLP+MK AAELAS+  KPWVLDP A   S FRL ACLEL+ 
Sbjct: 62  DFTPHIHALCVNVGTLTPDWLPSMKAAAELASQLRKPWVLDPAAVSCSGFRLKACLELIE 121

Query: 127 LKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVSGAVDIV 186
           LKPTV++GNGSEIIALS AS   + G DS H+S DA+EAAKSLA +SGA+VAVSGAVDIV
Sbjct: 122 LKPTVIKGNGSEIIALSSASRGQTKGADSSHESTDAIEAAKSLAMSSGAVVAVSGAVDIV 181

Query: 187 TDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGM 246
           TDG+QVIG HNG  MMQ+ITATGCS+  LI AFLA+D    LEAT SA+++FGIAGE+G 
Sbjct: 182 TDGKQVIGVHNGTKMMQQITATGCSLAGLIVAFLAIDSSRVLEATVSAMAVFGIAGELGE 241

Query: 247 DLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 281
            +A GPASLR HLID L+ LDE  ++ +V +T L
Sbjct: 242 AMANGPASLRMHLIDCLYGLDETTVLKRVNVTRL 275

[Close]

BLAST of MC05g0147 vs. ExPASy Swiss-Prot
Match: K7VCB9 (Hydroxyethylthiazole kinase OS=Zea mays OX=4577 GN=THIM PE=1 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.0e-87
Identity = 176/281 (62.63%), Postives = 220/281 (78.29%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           M+    E+E  +  WG RAW+ L+AVR+R+PL+QCITN VSMD+ AN LL+AGASPAM+H
Sbjct: 1   MDVGAKEEEMGQVWWGHRAWSLLSAVRARAPLVQCITNLVSMDIAANVLLAAGASPAMVH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           S+ E+PDFTP  DA+ +NVGTLS  WLP+M+ AA     AG+PWVLDPVAA AS FR+ A
Sbjct: 61  SLREVPDFTPRCDAVYVNVGTLSEDWLPSMRAAA----SAGRPWVLDPVAAAASGFRMKA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPS---MGVDSCHQSMDAVEAAKSLAQTSGAIV 180
           CLELL+L P VVRGN SEI+AL+  ST  S    GVDS H S+DA+EAAK+LA +S A+V
Sbjct: 121 CLELLSLCPAVVRGNASEILALASRSTAASSNFKGVDSSHCSVDAIEAAKALALSSSAVV 180

Query: 181 AVSGAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSI 240
           AVSGAVD VTDG+QVI   NGV MMQKITATGC+VTALIAAF+A++   A+ A A AL+I
Sbjct: 181 AVSGAVDFVTDGKQVISVSNGVPMMQKITATGCAVTALIAAFVAMEPSDAIVAAACALAI 240

Query: 241 FGIAGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKIT 279
           FG+ GE+GM+ AKGPASLR HLID+L+ L+E  + S+V+I+
Sbjct: 241 FGLVGEIGMESAKGPASLRMHLIDALYCLNEETVTSRVRIS 277

[Close]

BLAST of MC05g0147 vs. ExPASy Swiss-Prot
Match: B9JK65 (Hydroxyethylthiazole kinase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) OX=311403 GN=thiM PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 4.2e-57
Identity = 128/258 (49.61%), Postives = 166/258 (64.34%), Query Frame = 0


Query: 23  LAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELPDFTPNADALCINVGTL 82
           L A+R+  PL+QCITNFV+M++ AN +L+AGASPAM+H+ EE  +F   + AL IN+GTL
Sbjct: 12  LTAMRANPPLVQCITNFVAMNIAANVMLAAGASPAMVHAEEEAGEFAAISGALTINIGTL 71

Query: 83  SAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMACLELLNLKPTVVRGNGSEIIAL 142
           S  WL  M  AA+ A+KAGKPWVLDPVA  A+ FR  +  +LL L+PT++RGN SEIIAL
Sbjct: 72  STGWLAGMTSAAQAANKAGKPWVLDPVAHYATSFRRRSVAQLLELQPTIIRGNASEIIAL 131

Query: 143 SKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVSGAVDIVTDGEQVIGAHNGVAMM 202
           +   T    GVDS      A EAA  LAQ    IVAV+GA D VTDG +      G  +M
Sbjct: 132 A-GGTSRGQGVDSRDPVELAEEAAVQLAQKHRTIVAVTGATDFVTDGNKASRIEGGSPLM 191

Query: 203 QKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGMDLAKGPASLRTHLIDS 262
            ++TA GCS+T L+ AF A    H L+AT +AL+ F +AGE       GP S     +D+
Sbjct: 192 PQVTALGCSLTCLVGAFAATRPDHPLDATVAALAAFAVAGERAGRQVDGPGSFSWRFLDA 251

Query: 263 LHELDEAAIISQVKITSL 281
           L  LDE AI S+ ++ SL
Sbjct: 252 LAALDEKAIDSEARVISL 268

[Close]

BLAST of MC05g0147 vs. ExPASy Swiss-Prot
Match: Q1M5U3 (Hydroxyethylthiazole kinase OS=Rhizobium leguminosarum bv. viciae (strain 3841) OX=216596 GN=thiM PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 7.9e-56
Identity = 126/259 (48.65%), Postives = 168/259 (64.86%), Query Frame = 0


Query: 21  ATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELPDFTPNADALCINVG 80
           A L A+R + PL+QCITN+V+M++ AN LL+AGASPAM+H+ EE  +F   A AL IN+G
Sbjct: 9   AMLKAMREKPPLVQCITNYVAMNIAANVLLAAGASPAMVHAAEEAGEFAAIASALTINIG 68

Query: 81  TLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMACLELLNLKPTVVRGNGSEII 140
           TLS  W+  M+ AA+ A+ AGKPWVLDPVA  A+ FR  A  ELL LKPT++RGN SEII
Sbjct: 69  TLSTQWIDGMQAAAKAATSAGKPWVLDPVAHYATAFRRNAVAELLALKPTIIRGNASEII 128

Query: 141 ALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVSGAVDIVTDGEQVIGAHNGVA 200
           AL+   +    GVDS      A  +A+ LA+   A+VAV+GAVD VTDGE+ +    G A
Sbjct: 129 ALAGGESR-GQGVDSRDPVEQAEGSARWLAERQRAVVAVTGAVDFVTDGERAVRIEGGSA 188

Query: 201 MMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGMDLAKGPASLRTHLI 260
           +M ++TA GCS+T L+ AF A        AT +ALS F IAGE     A GP S     +
Sbjct: 189 LMPQVTALGCSLTCLVGAFAATAPEDIFGATVAALSTFAIAGEEAALGAAGPGSFSWRFL 248

Query: 261 DSLHELDEAAIISQVKITS 280
           D+L  LD   + ++ +I++
Sbjct: 249 DALAALDAETLDARARISA 266

[Close]

BLAST of MC05g0147 vs. ExPASy Swiss-Prot
Match: A3PM38 (Hydroxyethylthiazole kinase OS=Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) OX=349101 GN=thiM PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.3e-55
Identity = 125/249 (50.20%), Postives = 164/249 (65.86%), Query Frame = 0


Query: 23  LAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELPDFTPNADALCINVGTL 82
           L  +R  +PL+QCITNFV+M+++AN LL+AGASPAM+H  EE  +F   A AL IN+GT 
Sbjct: 8   LDTMRREAPLVQCITNFVAMNVVANVLLAAGASPAMVHDAEESGEFAAIAQALTINMGTP 67

Query: 83  SAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMACLELLNLKPTVVRGNGSEIIAL 142
           S  W+  M+ AA  A+ AG+PWVLDPVA GA+ FR      LL LKPTV+RGN SEI+AL
Sbjct: 68  SPRWVEGMEAAARGAAAAGRPWVLDPVAVGATAFRRGLGARLLALKPTVIRGNASEILAL 127

Query: 143 SKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVSGAVDIVTDGEQVIGAHNGVAMM 202
           + A T    G DS      A  AA+ LA++SGA+VAV+G VD VTDG + I   NG  +M
Sbjct: 128 AGAETR-GKGADSADPVAAAEAAAQRLAESSGAVVAVTGPVDFVTDGRRGIRCANGHPLM 187

Query: 203 QKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGMDLAKGPASLRTHLIDS 262
            ++TA GCS+T ++ AF A+      EATA+AL+ FG+AGE     A GP S +   +D+
Sbjct: 188 PRVTALGCSLTGIVGAFAAIRP--PFEATAAALAFFGLAGEEAAKTATGPGSFQVAFLDA 247

Query: 263 LHELDEAAI 272
           LH L   A+
Sbjct: 248 LHALSPEAL 253

[Close]

BLAST of MC05g0147 vs. NCBI nr
Match: XP_022157987.1 (hydroxyethylthiazole kinase [Momordica charantia] >XP_022157988.1 hydroxyethylthiazole kinase [Momordica charantia])

HSP 1 Score: 525 bits (1353), Expect = 1.72e-187
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA
Sbjct: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS
Sbjct: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL
Sbjct: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280

[Close]

BLAST of MC05g0147 vs. NCBI nr
Match: XP_022974515.1 (hydroxyethylthiazole kinase [Cucurbita maxima] >XP_022974516.1 hydroxyethylthiazole kinase [Cucurbita maxima])

HSP 1 Score: 481 bits (1238), Expect = 5.77e-170
Identity = 255/280 (91.07%), Postives = 266/280 (95.00%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK+ EDE QRRRWGAR W+TLAAVRSRSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQKEDEEQRRRWGARTWSTLAAVRSRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           S+EELPDFTPNADALCINVGTLSAAWLPAMK AAELA KAGKPWVLDPVAAGASKFR+MA
Sbjct: 61  SIEELPDFTPNADALCINVGTLSAAWLPAMKFAAELALKAGKPWVLDPVAAGASKFRMMA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLEL+ LKPTVVRGNGSEIIALSKASTE SMGVDS H+S DAVEAAKSLAQ SGAIVAVS
Sbjct: 121 CLELMQLKPTVVRGNGSEIIALSKASTESSMGVDSSHESTDAVEAAKSLAQNSGAIVAVS 180

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG+QVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALE TASALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALETTASALSIFGI 240

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGMD AKGPASLRTHLIDSL+ LDEAA+IS+VKI SL
Sbjct: 241 AGEMGMDAAKGPASLRTHLIDSLYGLDEAAVISRVKIASL 280

[Close]

BLAST of MC05g0147 vs. NCBI nr
Match: XP_038882134.1 (hydroxyethylthiazole kinase [Benincasa hispida])

HSP 1 Score: 477 bits (1228), Expect = 1.92e-168
Identity = 251/280 (89.64%), Postives = 267/280 (95.36%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK+NEDE QRR+WGARAWATLA VR+RSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQNEDEEQRRQWGARAWATLATVRTRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           SVEELPDFTPNADALCINVGTLS AWLPAMK AAELA KAGKPWVLDPVA GASKFR+MA
Sbjct: 61  SVEELPDFTPNADALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAVGASKFRMMA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLEL+NLKPTVVRGNGSEIIALSKAS E SMGVDSCH+S+DAVE AKSLAQ+SGAIVAVS
Sbjct: 121 CLELMNLKPTVVRGNGSEIIALSKASAESSMGVDSCHESVDAVEVAKSLAQSSGAIVAVS 180

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG+QVIGA NGVAMMQKITATGCSVTALIAAFLAVDQLHALEAT  ALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATTLALSIFGI 240

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGMD+AKGPASLRTHLIDSL+ LDEAA+IS+V+I+SL
Sbjct: 241 AGEMGMDMAKGPASLRTHLIDSLYGLDEAALISRVRISSL 280

[Close]

BLAST of MC05g0147 vs. NCBI nr
Match: KAG6597318.1 (Hydroxyethylthiazole kinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028785.1 Hydroxyethylthiazole kinase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 477 bits (1228), Expect = 1.92e-168
Identity = 253/280 (90.36%), Postives = 266/280 (95.00%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK+ EDE QRR+WGAR W+TLAAVRSRSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQIEDEEQRRQWGARTWSTLAAVRSRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           S+EELPDFTPN DALCINVGTLSAAWLPAMK AAELA KAGKPWVLDPVAAGASKFR+MA
Sbjct: 61  SIEELPDFTPNVDALCINVGTLSAAWLPAMKFAAELALKAGKPWVLDPVAAGASKFRMMA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLEL+ LKPTVVRGNGSEIIALSKASTE SMGVDS H+S DAVEAAKSLAQTSGAIVAVS
Sbjct: 121 CLELMKLKPTVVRGNGSEIIALSKASTESSMGVDSSHESTDAVEAAKSLAQTSGAIVAVS 180

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG+QVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALE TASALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALETTASALSIFGI 240

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGM+ AKGPASLRTHLIDSL+ LDEAA+IS+VKI SL
Sbjct: 241 AGEMGMETAKGPASLRTHLIDSLYGLDEAAVISRVKIASL 280

[Close]

BLAST of MC05g0147 vs. NCBI nr
Match: XP_022946989.1 (hydroxyethylthiazole kinase [Cucurbita moschata] >XP_022946998.1 hydroxyethylthiazole kinase [Cucurbita moschata])

HSP 1 Score: 475 bits (1223), Expect = 1.11e-167
Identity = 253/280 (90.36%), Postives = 266/280 (95.00%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK+ EDE QRR+WGAR W+TLAAVRSRSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQIEDEEQRRQWGARTWSTLAAVRSRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           S+EELPDFTPN DALCINVGTLSAAWLPAMK AAELA KAGKPWVLDPVAAGASKFR+MA
Sbjct: 61  SLEELPDFTPNVDALCINVGTLSAAWLPAMKFAAELALKAGKPWVLDPVAAGASKFRMMA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLEL+ LKPTVVRGNGSEIIALSKASTE SMGVDS H+S DAVEAAKSLAQTSGAIVAVS
Sbjct: 121 CLELMQLKPTVVRGNGSEIIALSKASTEFSMGVDSSHESTDAVEAAKSLAQTSGAIVAVS 180

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG+QVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALE TASALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALETTASALSIFGI 240

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGM+ AKGPASLRTHLIDSL+ LDEAA+IS+VKI SL
Sbjct: 241 AGEMGMETAKGPASLRTHLIDSLYGLDEAAVISRVKIASL 280

[Close]

BLAST of MC05g0147 vs. ExPASy TrEMBL
Match: A0A6J1DUV0 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Momordica charantia OX=3673 GN=LOC111024581 PE=3 SV=1)

HSP 1 Score: 525 bits (1353), Expect = 8.34e-188
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA
Sbjct: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS
Sbjct: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL
Sbjct: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280

[Close]

BLAST of MC05g0147 vs. ExPASy TrEMBL
Match: A0A6J1IBK3 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucurbita maxima OX=3661 GN=LOC111473194 PE=3 SV=1)

HSP 1 Score: 481 bits (1238), Expect = 2.79e-170
Identity = 255/280 (91.07%), Postives = 266/280 (95.00%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK+ EDE QRRRWGAR W+TLAAVRSRSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQKEDEEQRRRWGARTWSTLAAVRSRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           S+EELPDFTPNADALCINVGTLSAAWLPAMK AAELA KAGKPWVLDPVAAGASKFR+MA
Sbjct: 61  SIEELPDFTPNADALCINVGTLSAAWLPAMKFAAELALKAGKPWVLDPVAAGASKFRMMA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLEL+ LKPTVVRGNGSEIIALSKASTE SMGVDS H+S DAVEAAKSLAQ SGAIVAVS
Sbjct: 121 CLELMQLKPTVVRGNGSEIIALSKASTESSMGVDSSHESTDAVEAAKSLAQNSGAIVAVS 180

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG+QVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALE TASALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALETTASALSIFGI 240

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGMD AKGPASLRTHLIDSL+ LDEAA+IS+VKI SL
Sbjct: 241 AGEMGMDAAKGPASLRTHLIDSLYGLDEAAVISRVKIASL 280

[Close]

BLAST of MC05g0147 vs. ExPASy TrEMBL
Match: A0A6J1G5H2 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucurbita moschata OX=3662 GN=LOC111451002 PE=3 SV=1)

HSP 1 Score: 475 bits (1223), Expect = 5.38e-168
Identity = 253/280 (90.36%), Postives = 266/280 (95.00%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK+ EDE QRR+WGAR W+TLAAVRSRSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQIEDEEQRRQWGARTWSTLAAVRSRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           S+EELPDFTPN DALCINVGTLSAAWLPAMK AAELA KAGKPWVLDPVAAGASKFR+MA
Sbjct: 61  SLEELPDFTPNVDALCINVGTLSAAWLPAMKFAAELALKAGKPWVLDPVAAGASKFRMMA 120

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLEL+ LKPTVVRGNGSEIIALSKASTE SMGVDS H+S DAVEAAKSLAQTSGAIVAVS
Sbjct: 121 CLELMQLKPTVVRGNGSEIIALSKASTEFSMGVDSSHESTDAVEAAKSLAQTSGAIVAVS 180

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG+QVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALE TASALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALETTASALSIFGI 240

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGM+ AKGPASLRTHLIDSL+ LDEAA+IS+VKI SL
Sbjct: 241 AGEMGMETAKGPASLRTHLIDSLYGLDEAAVISRVKIASL 280

[Close]

BLAST of MC05g0147 vs. ExPASy TrEMBL
Match: A0A5A7U6A2 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002350 PE=3 SV=1)

HSP 1 Score: 474 bits (1220), Expect = 3.18e-167
Identity = 249/280 (88.93%), Postives = 269/280 (96.07%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK +EDE QRR+WG RAWATL+AVR++SPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 21  MNSKHDEDEQQRRQWGTRAWATLSAVRTQSPLIQCITNFVSMDLMANTLLSAGASPAMLH 80

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           SVEELPDFTPNADALCINVGTLS AWLPAMK A+ELA KAGKPWVLDPVAAGASKFR+MA
Sbjct: 81  SVEELPDFTPNADALCINVGTLSPAWLPAMKFASELAVKAGKPWVLDPVAAGASKFRMMA 140

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLEL+NLKPTVVRGNGSEIIALSKAS + SMGVDSCH+S+DAVEAAKSLA +SGAIVAVS
Sbjct: 141 CLELMNLKPTVVRGNGSEIIALSKASLDSSMGVDSCHESVDAVEAAKSLAHSSGAIVAVS 200

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG++VIGA NGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 201 GAVDIVTDGKRVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 260

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGMD+AKGPASLRTHLIDSL+ LDEAAIIS+++I+SL
Sbjct: 261 AGEMGMDVAKGPASLRTHLIDSLYGLDEAAIISRIRISSL 300

[Close]

BLAST of MC05g0147 vs. ExPASy TrEMBL
Match: A0A0A0L6V8 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis sativus OX=3659 GN=Csa_3G133300 PE=3 SV=1)

HSP 1 Score: 474 bits (1220), Expect = 3.18e-167
Identity = 249/280 (88.93%), Postives = 268/280 (95.71%), Query Frame = 0


Query: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK +EDE QRR+WG RAWATL+A+R+RSPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 21  MNSKHDEDEQQRRQWGTRAWATLSALRARSPLIQCITNFVSMDLMANTLLSAGASPAMLH 80

Query: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120
           SVEELPDFTPNADALCINVGTLS AWLPAMK A ELA KAGKPWVLDPVA GASKFR+MA
Sbjct: 81  SVEELPDFTPNADALCINVGTLSPAWLPAMKFAGELAVKAGKPWVLDPVAVGASKFRMMA 140

Query: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180
           CLEL++LKPTVVRGNGSEIIALSKAS + SMGVDSCH+S+DAVEAAKSLAQ+SGAIVAVS
Sbjct: 141 CLELMSLKPTVVRGNGSEIIALSKASLDSSMGVDSCHESVDAVEAAKSLAQSSGAIVAVS 200

Query: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG+QVIGA NGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 201 GAVDIVTDGKQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 260

Query: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280
           AGEMGMD+AKGPASLRTHLIDSL+ LDEAAIIS+++I+SL
Sbjct: 261 AGEMGMDVAKGPASLRTHLIDSLYGLDEAAIISRIRISSL 300

[Close]

BLAST of MC05g0147 vs. TAIR 10
Match: AT3G24030.1 (hydroxyethylthiazole kinase family protein )

HSP 1 Score: 356.7 bits (914), Expect = 1.7e-98
Identity = 182/274 (66.42%), Postives = 219/274 (79.93%), Query Frame = 0


Query: 7   EDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELP 66
           E ++++  W +  WA L AVR +SPL+QCITNFVSMDL+ANTLLSAGASPAM+HSV E+P
Sbjct: 2   ESKSEQNEWSSGVWAHLTAVRQQSPLVQCITNFVSMDLVANTLLSAGASPAMVHSVVEIP 61

Query: 67  DFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMACLELLN 126
           DFTP+  ALC+NVGTL+  WLP+MK AAELAS+  KPWVLDP A   S FRL ACLEL+ 
Sbjct: 62  DFTPHIHALCVNVGTLTPDWLPSMKAAAELASQLRKPWVLDPAAVSCSGFRLKACLELIE 121

Query: 127 LKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVSGAVDIV 186
           LKPTV++GNGSEIIALS AS   + G DS H+S DA+EAAKSLA +SGA+VAVSGAVDIV
Sbjct: 122 LKPTVIKGNGSEIIALSSASRGQTKGADSSHESTDAIEAAKSLAMSSGAVVAVSGAVDIV 181

Query: 187 TDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGM 246
           TDG+QVIG HNG  MMQ+ITATGCS+  LI AFLA+D    LEAT SA+++FGIAGE+G 
Sbjct: 182 TDGKQVIGVHNGTKMMQQITATGCSLAGLIVAFLAIDSSRVLEATVSAMAVFGIAGELGE 241

Query: 247 DLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 281
            +A GPASLR HLID L+ LDE  ++ +V +T L
Sbjct: 242 AMANGPASLRMHLIDCLYGLDETTVLKRVNVTRL 275

The following BLAST results are available for this feature:

BLAST of MC05g0147 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIQ4	2.4e-97	66.42	Hydroxyethylthiazole kinase OS=Arabidopsis thaliana OX=3702 GN=THIM PE=1 SV=1	[more]
K7VCB9	3.0e-87	62.63	Hydroxyethylthiazole kinase OS=Zea mays OX=4577 GN=THIM PE=1 SV=1	[more]
B9JK65	4.2e-57	49.61	Hydroxyethylthiazole kinase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-...	[more]
Q1M5U3	7.9e-56	48.65	Hydroxyethylthiazole kinase OS=Rhizobium leguminosarum bv. viciae (strain 3841) ...	[more]
A3PM38	1.3e-55	50.20	Hydroxyethylthiazole kinase OS=Rhodobacter sphaeroides (strain ATCC 17029 / ATH ...	[more]

BLAST of MC05g0147 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157987.1	1.72e-187	100.00	hydroxyethylthiazole kinase [Momordica charantia] >XP_022157988.1 hydroxyethylth...	[more]
XP_022974515.1	5.77e-170	91.07	hydroxyethylthiazole kinase [Cucurbita maxima] >XP_022974516.1 hydroxyethylthiaz...	[more]
XP_038882134.1	1.92e-168	89.64	hydroxyethylthiazole kinase [Benincasa hispida]	[more]
KAG6597318.1	1.92e-168	90.36	Hydroxyethylthiazole kinase, partial [Cucurbita argyrosperma subsp. sororia] >KA...	[more]
XP_022946989.1	1.11e-167	90.36	hydroxyethylthiazole kinase [Cucurbita moschata] >XP_022946998.1 hydroxyethylthi...	[more]

BLAST of MC05g0147 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUV0	8.34e-188	100.00	4-methyl-5-beta-hydroxyethylthiazole kinase OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1IBK3	2.79e-170	91.07	4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1G5H2	5.38e-168	90.36	4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A5A7U6A2	3.18e-167	88.93	4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0L6V8	3.18e-167	88.93	4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]

BLAST of MC05g0147 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24030.1	1.7e-98	66.42	hydroxyethylthiazole kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000417	Hydroxyethylthiazole kinase	PRINTS	PR01099	HYETHTZKNASE	coord: 176..193 score: 44.44 coord: 44..65 score: 48.48 coord: 31..38 score: 63.89 coord: 131..145 score: 42.22 coord: 101..118 score: 56.79 coord: 75..83 score: 61.73
IPR000417	Hydroxyethylthiazole kinase	PFAM	PF02110	HK	coord: 23..267 e-value: 1.0E-69 score: 234.7
IPR000417	Hydroxyethylthiazole kinase	PIRSF	PIRSF000513	Thz_kinase	coord: 17..280 e-value: 7.7E-93 score: 308.3
IPR000417	Hydroxyethylthiazole kinase	HAMAP	MF_00228	Thz_kinase	coord: 25..272 score: 29.503048
IPR000417	Hydroxyethylthiazole kinase	CDD	cd01170	THZ_kinase	coord: 23..264 e-value: 8.4877E-107 score: 307.929
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 2..278 e-value: 5.8E-86 score: 290.1
IPR029056	Ribokinase-like	SUPERFAMILY	53613	Ribokinase-like	coord: 16..277

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0147.1	MC05g0147.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0009229	thiamine diphosphate biosynthetic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004417	hydroxyethylthiazole kinase activity
molecular_function	GO:0046872	metal ion binding