MC04g0462 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0462
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptiontranscription factor bHLH63-like
LocationMC04: 3731823 .. 3733677 (-)
RNA-Seq ExpressionMC04g0462
SyntenyMC04g0462
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAGAGCATTGCCGGAGATTTTGCACTGTTTTAACACTGCCGGGAACTGTCCTGATACTCTCACTGTTCTCGAGAGGCAGAGGGCTCGTTTGAAATGGCAGCAAGATCAGTTCTTGCAGCAACAGAGTCAGGGCTGTTTCAATAGGGCCGATTACGGCGGATTTCCGCCGCCGGTGGCTGTTCCGCCGGATCACCACCTTCCGGGGTTTATGGGGTTCATGAGCGGCGGCGGCGAGGGTGGCAGTGGTGGTGGGGCGTCCCTTGTTGAGATGGTAATGGGGGCGGTGAAGCCTGACCCTGGCTTGGAAGATGGGTGGTCTGAAATGGGCAAGTTTGATCCTTCTCTGTTGCTGAATGCCACTGCTTATGAACTCAATTCTTCTCTCTCTAGAACGTCCAGCTGCCCGCCGGCGGTGCCGGAGAAGGTGGGGTCGGCGGCCGGAAGGGAGAGCTTCAAGAAAAGGAAAGCTGAGAAAGTTCAGAACAACAACAGCAGCAGCAACAACAACAAGGCAAGTTTCTATAATATGATCTCTCTGTTTGTTTCTTTTTCAAGACCCAAAAGAGGATTCTAATTCTTTAGACCCAGATGGGAAAAGAATGGCCAAAAGTTTTCAAGGAGTTGTAACAGATTAATGGTGGATTTTGCAGGTGACTGTGGAGGAAGATAACAACAACAACAGCAACAAAGAGATCAAGAGGATCAAAGCAAGTGCAGAAGAAGGAGAATCCAAAGCCACAGATCAAAACAGCACCAAAAACAACAACTCAACCACCACCACGACAACAAACAACAACAGAGAAACTTCAGCTGATACCTCAAAAGAAAACTCCAAAGCTTCAGAGGTTCAAAAGCCTGATTACATTCATGTCAGAGCTCGCCGTGGCCAAGCCACTGATAGCCATAGCTTAGCAGAAAGAGTAAGCCAAAAAAAACATCCAATTTCTTCTTCTGGGTTGCTTTCTTTTTCTTTGATTTGTTTGTTTGTTATGAACTTGGGCATTTTCTCTTTTGATAATCAAGTTAATTTTTGGTTTCATGTAGGCTAGAAGGGAAAAAATCAGTGAGAGAATGAAGTATCTGCAAGATTTAGTACCAGGGTGTAACAAAATCACTGGAAAAGCTGGAATGCTGGACGAGATCATCAATTATGTTCAATCTCTTCAAAGACAAGTGGAGGTGAAAACTCAAATTTTCTCAATCACCTTAAAAATGGTGCTCAACTTGCTCTGAAATCTAAACTTCTCTCTGTGGCATTTTATCAAACAGTTCTTGTCCATGAAGTTAGCCGCCGTTAATCCAAGGTTCGACTTCAATGTCGATGATCTCTTCGGCAAAGAGGTAACAAAACTAAACGATTTCAAAAGTTCAAACTCATTTAATCAGAAAAATCAACAACCAGAAGCTAAAATTTTCCCGGGAAACAAACAGGTTTTCCCCCCTCCTTGCACCACCGCCAACTTCCCGGCGATCGGCGGTATCTCCTCGGAGATGACAGATCATTCTTCCTACTTACAATTTAATCCAAACAATCAACAAATCGTCCCGGGTTGTGGATTAGAAATGGGAATGAATACCTCCGACGTCGGTCTCCGAAGATCCATTAGCGCCCCTGTTTCTTTCCCGGAAAATTTCCTCGATTCCTCCTGCCTCACCGTAAGTTCTCTTCAAACTCCGCCGAATTCGATTCCGTTTCGCTCATTTTCTGTTCCTAATTACATCAAATCAATCAATCGCTGCAGCAATTCCAACCCTCCTCAGCGTGGGACGTCGATTTGCAGAATCTGTACAATGTGGGTTTCGATCAAGCAAGATCATCAAACGCCTTTTCTTCTCAACCATATACAGGT

mRNA sequence

ATGAACAGAGCATTGCCGGAGATTTTGCACTGTTTTAACACTGCCGGGAACTGTCCTGATACTCTCACTGTTCTCGAGAGGCAGAGGGCTCGTTTGAAATGGCAGCAAGATCAGTTCTTGCAGCAACAGAGTCAGGGCTGTTTCAATAGGGCCGATTACGGCGGATTTCCGCCGCCGGTGGCTGTTCCGCCGGATCACCACCTTCCGGGGTTTATGGGGTTCATGAGCGGCGGCGGCGAGGGTGGCAGTGGTGGTGGGGCGTCCCTTGTTGAGATGGTAATGGGGGCGGTGAAGCCTGACCCTGGCTTGGAAGATGGGTGGTCTGAAATGGGCAAGTTTGATCCTTCTCTGTTGCTGAATGCCACTGCTTATGAACTCAATTCTTCTCTCTCTAGAACGTCCAGCTGCCCGCCGGCGGTGCCGGAGAAGGTGGGGTCGGCGGCCGGAAGGGAGAGCTTCAAGAAAAGGAAAGCTGAGAAAGTTCAGAACAACAACAGCAGCAGCAACAACAACAAGGCAAGTTTCTATAATATGATCTCTCTGTTTGTGACTGTGGAGGAAGATAACAACAACAACAGCAACAAAGAGATCAAGAGGATCAAAGCAAGTGCAGAAGAAGGAGAATCCAAAGCCACAGATCAAAACAGCACCAAAAACAACAACTCAACCACCACCACGACAACAAACAACAACAGAGAAACTTCAGCTGATACCTCAAAAGAAAACTCCAAAGCTTCAGAGGTTCAAAAGCCTGATTACATTCATGTCAGAGCTCGCCGTGGCCAAGCCACTGATAGCCATAGCTTAGCAGAAAGAGCTAGAAGGGAAAAAATCAGTGAGAGAATGAAGTATCTGCAAGATTTAGTACCAGGGTGTAACAAAATCACTGGAAAAGCTGGAATGCTGGACGAGATCATCAATTATGTTCAATCTCTTCAAAGACAAGTGGAGTTCTTGTCCATGAAGTTAGCCGCCGTTAATCCAAGGTTCGACTTCAATGTCGATGATCTCTTCGGCAAAGAGGTTTTCCCCCCTCCTTGCACCACCGCCAACTTCCCGGCGATCGGCGGTATCTCCTCGGAGATGACAGATCATTCTTCCTACTTACAATTTAATCCAAACAATCAACAAATCGTCCCGGGTTGTGGATTAGAAATGGGAATGAATACCTCCGACGTCGGTCTCCGAAGATCCATTAGCGCCCCTGTTTCTTTCCCGGAAAATTTCCTCGATTCCTCCTGCCTCACCCAATTCCAACCCTCCTCAGCGTGGGACGTCGATTTGCAGAATCTGTACAATGTGGGTTTCGATCAAGCAAGATCATCAAACGCCTTTTCTTCTCAACCATATACAGGT

Coding sequence (CDS)

ATGAACAGAGCATTGCCGGAGATTTTGCACTGTTTTAACACTGCCGGGAACTGTCCTGATACTCTCACTGTTCTCGAGAGGCAGAGGGCTCGTTTGAAATGGCAGCAAGATCAGTTCTTGCAGCAACAGAGTCAGGGCTGTTTCAATAGGGCCGATTACGGCGGATTTCCGCCGCCGGTGGCTGTTCCGCCGGATCACCACCTTCCGGGGTTTATGGGGTTCATGAGCGGCGGCGGCGAGGGTGGCAGTGGTGGTGGGGCGTCCCTTGTTGAGATGGTAATGGGGGCGGTGAAGCCTGACCCTGGCTTGGAAGATGGGTGGTCTGAAATGGGCAAGTTTGATCCTTCTCTGTTGCTGAATGCCACTGCTTATGAACTCAATTCTTCTCTCTCTAGAACGTCCAGCTGCCCGCCGGCGGTGCCGGAGAAGGTGGGGTCGGCGGCCGGAAGGGAGAGCTTCAAGAAAAGGAAAGCTGAGAAAGTTCAGAACAACAACAGCAGCAGCAACAACAACAAGGCAAGTTTCTATAATATGATCTCTCTGTTTGTGACTGTGGAGGAAGATAACAACAACAACAGCAACAAAGAGATCAAGAGGATCAAAGCAAGTGCAGAAGAAGGAGAATCCAAAGCCACAGATCAAAACAGCACCAAAAACAACAACTCAACCACCACCACGACAACAAACAACAACAGAGAAACTTCAGCTGATACCTCAAAAGAAAACTCCAAAGCTTCAGAGGTTCAAAAGCCTGATTACATTCATGTCAGAGCTCGCCGTGGCCAAGCCACTGATAGCCATAGCTTAGCAGAAAGAGCTAGAAGGGAAAAAATCAGTGAGAGAATGAAGTATCTGCAAGATTTAGTACCAGGGTGTAACAAAATCACTGGAAAAGCTGGAATGCTGGACGAGATCATCAATTATGTTCAATCTCTTCAAAGACAAGTGGAGTTCTTGTCCATGAAGTTAGCCGCCGTTAATCCAAGGTTCGACTTCAATGTCGATGATCTCTTCGGCAAAGAGGTTTTCCCCCCTCCTTGCACCACCGCCAACTTCCCGGCGATCGGCGGTATCTCCTCGGAGATGACAGATCATTCTTCCTACTTACAATTTAATCCAAACAATCAACAAATCGTCCCGGGTTGTGGATTAGAAATGGGAATGAATACCTCCGACGTCGGTCTCCGAAGATCCATTAGCGCCCCTGTTTCTTTCCCGGAAAATTTCCTCGATTCCTCCTGCCTCACCCAATTCCAACCCTCCTCAGCGTGGGACGTCGATTTGCAGAATCTGTACAATGTGGGTTTCGATCAAGCAAGATCATCAAACGCCTTTTCTTCTCAACCATATACAGGT

Protein sequence

MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFLQQQSQGCFNRADYGGFPPPVAVPPDHHLPGFMGFMSGGGEGGSGGGASLVEMVMGAVKPDPGLEDGWSEMGKFDPSLLLNATAYELNSSLSRTSSCPPAVPEKVGSAAGRESFKKRKAEKVQNNNSSSNNNKASFYNMISLFVTVEEDNNNNSNKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFPENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG
Homology
BLAST of MC04g0462 vs. ExPASy Swiss-Prot
Match: Q8GY61 (Transcription factor bHLH63 OS=Arabidopsis thaliana OX=3702 GN=BHLH63 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.7e-44
Identity = 133/271 (49.08%), Postives = 153/271 (56.46%), Query Frame = 0

Query: 182 FVTVEEDNNNNSNKEIKRIKASAEEGE---SKATDQN--STKNNNSTTTTTTNNNRETSA 241
           F T  +D N    K         EEGE   SK T+QN  STK+               S 
Sbjct: 96  FDTETKDCNEKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSN 155

Query: 242 DTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKIT 301
           D+SK      E++K DYIHVRARRGQATDSHS+AER RREKISERMK+LQDLVPGC+KIT
Sbjct: 156 DSSK---VTKELEKTDYIHVRARRGQATDSHSIAERVRREKISERMKFLQDLVPGCDKIT 215

Query: 302 GKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTANFPAI- 361
           GKAGMLDEIINYVQSLQRQ+EFLSMKLA VNPR DF++DD+F KEV   P T    P + 
Sbjct: 216 GKAGMLDEIINYVQSLQRQIEFLSMKLAIVNPRPDFDMDDIFAKEVASTPMTVVPSPEMV 275

Query: 362 ----------GGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSF 421
                      G SSEM  +S YL  NP  Q           +NTS        S P+  
Sbjct: 276 LSGYSHEMVHSGYSSEMV-NSGYLHVNPMQQ-----------VNTS--------SDPL-- 334

Query: 422 PENFLDSSCLTQFQPSSAWDVDLQNLY-NVG 436
                  SC    +  S WD  +QNLY N+G
Sbjct: 336 -------SCFNNGEAPSMWDSHVQNLYGNLG 334

BLAST of MC04g0462 vs. ExPASy Swiss-Prot
Match: Q9ZPW3 (Transcription factor HBI1 OS=Arabidopsis thaliana OX=3702 GN=HBI1 PE=1 SV=3)

HSP 1 Score: 154.8 bits (390), Expect = 2.3e-36
Identity = 93/149 (62.42%), Postives = 112/149 (75.17%), Query Frame = 0

Query: 194 NKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRETSADTSKENSK-ASEVQKPD 253
           NK    +K + EE +++   +   +  +S    +   N E S+DTSKE SK ASE QK D
Sbjct: 123 NKRKPEVK-TREEQKTEKKIKVEAETESSMKGKSNMGNTEASSDTSKETSKGASENQKLD 182

Query: 254 YIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSL 313
           YIHVRARRGQATD HSLAERARREKIS++MKYLQD+VPGCNK+TGKAGMLDEIINYVQ L
Sbjct: 183 YIHVRARRGQATDRHSLAERARREKISKKMKYLQDIVPGCNKVTGKAGMLDEIINYVQCL 242

Query: 314 QRQVEFLSMKLAAVNPRFDFNVDDLFGKE 342
           QRQVEFLSMKLA +NP  +  V+D+  K+
Sbjct: 243 QRQVEFLSMKLAVLNPELELAVEDVSVKQ 270

BLAST of MC04g0462 vs. ExPASy Swiss-Prot
Match: Q93VJ4 (Transcription factor BEE 2 OS=Arabidopsis thaliana OX=3702 GN=BEE2 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.6e-35
Identity = 85/157 (54.14%), Postives = 112/157 (71.34%), Query Frame = 0

Query: 185 VEEDNNNNSNKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRETSADTSKENSK 244
           +E   NN  ++ I        EG+++  ++   K  + T  +    +  ++ +TS     
Sbjct: 76  MEPVKNNGHSRAITLQNKRKPEGKTEKREKKKIKAEDETEPSMKGKSNMSNTETS----- 135

Query: 245 ASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKITGKAGMLDE 304
            SE+QKPDYIHVRARRG+ATD HSLAERARREKIS++MK LQD+VPGCNK+TGKAGMLDE
Sbjct: 136 -SEIQKPDYIHVRARRGEATDRHSLAERARREKISKKMKCLQDIVPGCNKVTGKAGMLDE 195

Query: 305 IINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKE 342
           IINYVQSLQ+QVEFLSMKL+ +NP  + ++DDL  K+
Sbjct: 196 IINYVQSLQQQVEFLSMKLSVINPELECHIDDLSAKQ 226

BLAST of MC04g0462 vs. ExPASy Swiss-Prot
Match: Q9CAA9 (Transcription factor bHLH49 OS=Arabidopsis thaliana OX=3702 GN=BHLH49 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.6e-35
Identity = 107/256 (41.80%), Postives = 149/256 (58.20%), Query Frame = 0

Query: 188 DNNNNSNKEIKRIKASAEEGESKATDQNSTKNNNS-----TTTTTTNNNRETSADTSKEN 247
           + ++N+ K  +  + ++E  +S  + Q+  + +N+         + N+  + S    ++ 
Sbjct: 230 ETSSNTKKRKRNGQKNSEAAQSHRSQQSEEEPDNNGDEKRNDEQSPNSPGKKSNSGKQQG 289

Query: 248 SKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKITGKAGML 307
            ++S+  K  YIHVRARRGQAT+SHSLAER RREKISERMK+LQDLVPGCNK+TGKA ML
Sbjct: 290 KQSSDPPKDGYIHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPGCNKVTGKAVML 349

Query: 308 DEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTANFPAIGGISSEM 367
           DEIINYVQSLQRQVEFLSMKLA VNP+ DFN++ L  K+                     
Sbjct: 350 DEIINYVQSLQRQVEFLSMKLATVNPQMDFNLEGLLAKDAL----------------QLR 409

Query: 368 TDHSSYLQFNPNNQQIVPGC--GLEMGMNTSDVGLRRSISAPVSFPENFLDSSCLTQFQP 427
              SS   F PN     P    G  M    S +G  R+I++P+S P N        + Q 
Sbjct: 410 AGSSSTTPFPPNMSMAYPPLPHGF-MQQTLSSIG--RTITSPLS-PMN-----GGFKRQE 460

Query: 428 SSAWDVDLQNLYNVGF 437
           ++ W+ DLQN+ ++ +
Sbjct: 470 TNGWEGDLQNVIHINY 460

BLAST of MC04g0462 vs. ExPASy Swiss-Prot
Match: Q9SRT2 (Transcription factor bHLH62 OS=Arabidopsis thaliana OX=3702 GN=BHLH62 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.1e-34
Identity = 128/344 (37.21%), Postives = 174/344 (50.58%), Query Frame = 0

Query: 122 TAYELNSSLSRTSSCPP-------AVPEKVGSAAGRESFKKRKAEKVQNNNSSSNNNKAS 181
           TA   NS  +   S PP            +   +G   F +R A      + S N+   S
Sbjct: 103 TASNGNSCYATPMSSPPPGSMMETKTTTPMAELSGDPGFAERAARFSCFGSRSFNSRTNS 162

Query: 182 FY--NMISLFVTVEEDNNNNSNKEIKRIKASAEEGESK---ATDQNSTKNNNSTTTTTTN 241
            +  N      T E+    +S+   K + +    GES    +  + +    NS +  +++
Sbjct: 163 PFPINNEPPITTNEKMPRVSSSPVFKPLASHVPAGESSGELSRKRKTKSKQNSPSAVSSS 222

Query: 242 NNRETSADTSKENSKASEVQ---------KPDYIHVRARRGQATDSHSLAERARREKISE 301
              E   D+  +  K SE             DYIHVRARRGQATDSHSLAER RREKISE
Sbjct: 223 KEIEEKEDSDPKRCKKSEENGDKTKSIDPYKDYIHVRARRGQATDSHSLAERVRREKISE 282

Query: 302 RMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGK 361
           RMK LQDLVPGCNK+TGKA MLDEIINYVQSLQRQVEFLSMKL++VN R DFN+D L  K
Sbjct: 283 RMKLLQDLVPGCNKVTGKALMLDEIINYVQSLQRQVEFLSMKLSSVNTRLDFNMDALLSK 342

Query: 362 EVFPPPCTTANFPAIGGISSE----MTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLR 421
           ++FP      +   +  + S     + DH +       N Q+ P       +N  +    
Sbjct: 343 DIFPSSNNLMHHQQVLQLDSSAETLLGDHHN------KNLQLNPDISSNNVINPLETSET 402

Query: 422 RSISAPVSFPENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQAR 441
           RS  + +    +F DS  ++Q+   S  + DL ++ ++GF Q R
Sbjct: 403 RSFISHLPTLAHFTDS--ISQYSTFS--EDDLHSIIHMGFAQNR 436

BLAST of MC04g0462 vs. NCBI nr
Match: XP_038888827.1 (transcription factor bHLH63-like [Benincasa hispida])

HSP 1 Score: 689 bits (1778), Expect = 1.94e-246
Identity = 383/463 (82.72%), Postives = 404/463 (87.26%), Query Frame = 0

Query: 1   MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFLQQQ----SQGCFNRADYGG- 60
           MNRALPE+LHC NTAGNC DTL+VLERQRARLKWQQDQ LQQQ    +Q CFNR DYGG 
Sbjct: 1   MNRALPEMLHCLNTAGNCSDTLSVLERQRARLKWQQDQLLQQQQQPLNQSCFNRVDYGGG 60

Query: 61  FPPPVAVPPDHHLPGFMGFMSGGGEGGSGG-GASLVEMVMGAVKPDPGLEDGWSEMGKFD 120
           FP PV+VP D+ L GF GFMSG   GGSGG G SLVEMVMGAVKPDPGLEDGWS M KFD
Sbjct: 61  FPSPVSVPADY-LTGFPGFMSG--SGGSGGAGLSLVEMVMGAVKPDPGLEDGWSGMSKFD 120

Query: 121 PSLLLNATAYELNSSLSRTSSCPPAVP----EKVGSAAGRESFKKRKAEKVQNNNSSSNN 180
           PSLLLN TAYE+NSSLSRTSSCPPAV     EK+GS AGRE+FKKRKAEKV N  +++N+
Sbjct: 121 PSLLLNGTAYEINSSLSRTSSCPPAVAPTVVEKMGSVAGRETFKKRKAEKVHNTTNNNNS 180

Query: 181 NKASFYNMISLFVTVEEDNNNNSNKEIKRIKASAEEGE-SKATDQNSTKNNNSTTTTTTN 240
           +K          VTVEEDNN  S+KE KRIKAS+EEGE SK TDQNSTKNN  +TTTTTN
Sbjct: 181 SK----------VTVEEDNN--SSKE-KRIKASSEEGELSKTTDQNSTKNN--STTTTTN 240

Query: 241 NNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLV 300
           NNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLV
Sbjct: 241 NNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLV 300

Query: 301 PGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTT 360
           PGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPR DFNVDDLF KEVFPP CTT
Sbjct: 301 PGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNVDDLFNKEVFPPSCTT 360

Query: 361 ANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFPENF 420
           ANFPAIGG+SSEMTD SS+LQFNPNNQ +V  CGLEMG+NTSDV LRR+ISAPVSFPENF
Sbjct: 361 ANFPAIGGMSSEMTDPSSFLQFNPNNQPMVSCCGLEMGINTSDVALRRTISAPVSFPENF 420

Query: 421 LDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           LDSSCLTQFQPSS WDVDLQNLYNVGFDQ RSSNAFSSQPYTG
Sbjct: 421 LDSSCLTQFQPSSGWDVDLQNLYNVGFDQGRSSNAFSSQPYTG 445

BLAST of MC04g0462 vs. NCBI nr
Match: XP_011658545.1 (transcription factor bHLH63 [Cucumis sativus] >KGN43145.1 hypothetical protein Csa_020359 [Cucumis sativus])

HSP 1 Score: 671 bits (1730), Expect = 3.91e-239
Identity = 368/458 (80.35%), Postives = 388/458 (84.72%), Query Frame = 0

Query: 1   MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFLQQQ-SQGCFNRADYGG-FPP 60
           MNRALPE+LHC N+ GNC DTL+VLERQRARLKWQQD   QQ  +Q CF+RADYGG FPP
Sbjct: 2   MNRALPEMLHCLNSTGNCSDTLSVLERQRARLKWQQDMLFQQPLNQSCFSRADYGGGFPP 61

Query: 61  PVAVPPDHHLPGFMGFMSGGGEGGSGGGASLVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 120
           PV+VP DH L GF G+MSGGG  G GGG S VEMVMGAVKPDPGLEDGWSEMGKFDPSLL
Sbjct: 62  PVSVPADH-LTGFPGYMSGGG--GVGGGLSHVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 121

Query: 121 LNATAYELNSSLSRTSSC----PPAVPEKVGSAAGRESFKKRKAEKVQNNNSSSNNNKAS 180
           LN TA ELNSSLSRTSSC     P V EK+GS AGRESFKKRKAEK  N  +++N N   
Sbjct: 122 LNPTACELNSSLSRTSSCLPVVAPTVAEKMGSMAGRESFKKRKAEKAHNTTTTANTNNNK 181

Query: 181 FYNMISLFVTVEEDNNNNSNKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRET 240
                   VTVEED NNNS +  KRIK S+E   SK TDQN TKNN ST TTTTNNNRET
Sbjct: 182 --------VTVEEDENNNSKE--KRIKTSSEGELSKTTDQNGTKNN-STITTTTNNNRET 241

Query: 241 SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK 300
           SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK
Sbjct: 242 SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK 301

Query: 301 ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTANFPA 360
           ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPR DFNVDDLF KEVFPP CT ANFP+
Sbjct: 302 ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNVDDLFNKEVFPPSCTAANFPS 361

Query: 361 IGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFPENFLDSSC 420
           +GG+SSEMTD SSYLQFNPNNQQ+   CGLEMG+NTS V LRR+ISAPVSFPENFLDSSC
Sbjct: 362 VGGMSSEMTDPSSYLQFNPNNQQMDSCCGLEMGINTSHVALRRTISAPVSFPENFLDSSC 421

Query: 421 LTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           LTQFQPSS WDVDLQN+YNVGFDQ RSSNAFSS PYTG
Sbjct: 422 LTQFQPSSGWDVDLQNMYNVGFDQGRSSNAFSSHPYTG 445

BLAST of MC04g0462 vs. NCBI nr
Match: XP_008448085.1 (PREDICTED: transcription factor bHLH49-like [Cucumis melo])

HSP 1 Score: 676 bits (1745), Expect = 9.15e-239
Identity = 372/458 (81.22%), Postives = 390/458 (85.15%), Query Frame = 0

Query: 1   MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFLQQQ-SQGCFNRADYGG-FPP 60
           MNRALPE+LHC NT GNC DTL+VLERQRARLKWQQDQ  QQ  +Q CFNRADYGG FPP
Sbjct: 174 MNRALPEMLHCLNTNGNCSDTLSVLERQRARLKWQQDQLFQQPLNQSCFNRADYGGGFPP 233

Query: 61  PVAVPPDHHLPGFMGFMSGGGEGGSGGGASLVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 120
           PV+VP DH L GF G+MSGGG GG  GG + VEMVMGAVKPDPGLEDGWSEMGKFDPSLL
Sbjct: 234 PVSVPVDH-LTGFPGYMSGGGGGG--GGLAHVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 293

Query: 121 LNATAYELNSSLSRTSSCPPAVP----EKVGSAAGRESFKKRKAEKVQNNNSSSNNNKAS 180
           LNATA ELNSSLSRTSSC P V     EK+GS AGRESFKKRKAEK  N  +++N+N   
Sbjct: 294 LNATACELNSSLSRTSSCLPVVAPTAAEKMGSVAGRESFKKRKAEKAHNTTTTNNSNNK- 353

Query: 181 FYNMISLFVTVEEDNNNNSNKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRET 240
                   VTVEED NN+  K   RIK S+E   SK TDQN TKNN ST TTTTNNNRET
Sbjct: 354 --------VTVEEDENNSKEK---RIKTSSEGELSKTTDQNGTKNN-STITTTTNNNRET 413

Query: 241 SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK 300
           SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK
Sbjct: 414 SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK 473

Query: 301 ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTANFPA 360
           ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPR DFNVDDLF KEVFPP CT ANFPA
Sbjct: 474 ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNVDDLFNKEVFPPSCTAANFPA 533

Query: 361 IGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFPENFLDSSC 420
           IGG+SSEMTD SSYLQFNPNNQQ+   CGLEMG+NTS V LRR+ISAPVSFPENFLDSSC
Sbjct: 534 IGGMSSEMTDPSSYLQFNPNNQQMDSCCGLEMGINTSHVALRRTISAPVSFPENFLDSSC 593

Query: 421 LTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           LTQFQPSS WDVDLQN+YNVGFDQ RSSNAFSSQPYTG
Sbjct: 594 LTQFQPSSGWDVDLQNMYNVGFDQGRSSNAFSSQPYTG 615

BLAST of MC04g0462 vs. NCBI nr
Match: XP_022135684.1 (transcription factor bHLH63-like [Momordica charantia])

HSP 1 Score: 662 bits (1708), Expect = 2.49e-237
Identity = 349/359 (97.21%), Postives = 349/359 (97.21%), Query Frame = 0

Query: 94  MGAVKPDPGLEDGWSEMGKFDPSLLLNATAYELNSSLSRTSSCPPAVPEKVGSAAGRESF 153
           MGAVKPDPGLEDGWSEMGKFDPSLLLNATAYELNSSLSRTSSCPPAVPEKVGSAAGRESF
Sbjct: 1   MGAVKPDPGLEDGWSEMGKFDPSLLLNATAYELNSSLSRTSSCPPAVPEKVGSAAGRESF 60

Query: 154 KKRKAEKVQNNNSSSNNNKASFYNMISLFVTVEEDNNNNSNKEIKRIKASAEEGESKATD 213
           KKRKAEKVQNNNSSSNNNK          VTVEEDNNNNSNKEIKRIKASAEEGESKATD
Sbjct: 61  KKRKAEKVQNNNSSSNNNK----------VTVEEDNNNNSNKEIKRIKASAEEGESKATD 120

Query: 214 QNSTKNNNSTTTTTTNNNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERA 273
           QNSTKNNNSTTTTTTNNNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERA
Sbjct: 121 QNSTKNNNSTTTTTTNNNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERA 180

Query: 274 RREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFN 333
           RREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFN
Sbjct: 181 RREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFN 240

Query: 334 VDDLFGKEVFPPPCTTANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDV 393
           VDDLFGKEVFPPPCTTANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDV
Sbjct: 241 VDDLFGKEVFPPPCTTANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDV 300

Query: 394 GLRRSISAPVSFPENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           GLRRSISAPVSFPENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG
Sbjct: 301 GLRRSISAPVSFPENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 349

BLAST of MC04g0462 vs. NCBI nr
Match: XP_023529229.1 (transcription factor bHLH63-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 605 bits (1561), Expect = 7.71e-214
Identity = 356/466 (76.39%), Postives = 366/466 (78.54%), Query Frame = 0

Query: 1   MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFL---QQQSQGCFN-RADYGGF 60
           MNRALPE+L   NTA NC DTLTVLERQRAR KWQQDQ L   QQQ Q  FN   DYG  
Sbjct: 1   MNRALPEML---NTAENCADTLTVLERQRARFKWQQDQLLYQQQQQQQQPFNGMMDYG-- 60

Query: 61  PPPVAVPPDHHLPGFMGFMSGGGEGGSGGGASLVEMVMGAVKPDPGLEDGWSEMGKFDPS 120
                 PP  HL GF GFMS  G+GG GGG SLVEMVMGAVKPDPGLEDGWS     DPS
Sbjct: 61  ---AGFPPSDHLTGFPGFMSAAGDGGGGGGLSLVEMVMGAVKPDPGLEDGWS-----DPS 120

Query: 121 LLLNA-TAYELNSSLSRTSSCPPAVPEKVGSAAGRESFKKRKAEKVQNNNSSSNNNKASF 180
           LLLN  TAYELNSSLSRTSSCPPAV     +AAGRESFKKRKAEKVQ             
Sbjct: 121 LLLNPPTAYELNSSLSRTSSCPPAV--NPAAAAGRESFKKRKAEKVQKPI---------- 180

Query: 181 YNMISLFVTVEEDNNNNSNKEIKRIKASAEEGE--------SKATDQNSTKNNNSTTTTT 240
                            +NKE K+IKAS+EE E        SK TDQNSTKNNNSTTTTT
Sbjct: 181 -----------------NNKE-KKIKASSEEEEEEEEEEELSKTTDQNSTKNNNSTTTTT 240

Query: 241 T-NNNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQ 300
           T NNNRETSADTSK    ASEV+KPDYIHVRARRGQATDSHSLAERARREKISERMKYLQ
Sbjct: 241 TTNNNRETSADTSK----ASEVKKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQ 300

Query: 301 DLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPP 360
           DLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPR DFNVDDLF KEVFPPP
Sbjct: 301 DLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNVDDLFLKEVFPPP 360

Query: 361 CTTANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFP 420
           CTTA F AIGGISSE+TD SSYLQFNPNNQQIVP CGLEMG+NTSDV LRR+ISAPVSFP
Sbjct: 361 CTTATFAAIGGISSEITDPSSYLQFNPNNQQIVPCCGLEMGINTSDVALRRTISAPVSFP 419

Query: 421 ENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           ENFLDSSCLTQFQ SS WDVDLQNLYNVGFDQ RSSNAFSSQPYTG
Sbjct: 421 ENFLDSSCLTQFQGSSGWDVDLQNLYNVGFDQGRSSNAFSSQPYTG 419

BLAST of MC04g0462 vs. ExPASy TrEMBL
Match: A0A0A0K0C8 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G000520 PE=4 SV=1)

HSP 1 Score: 671 bits (1730), Expect = 1.89e-239
Identity = 368/458 (80.35%), Postives = 388/458 (84.72%), Query Frame = 0

Query: 1   MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFLQQQ-SQGCFNRADYGG-FPP 60
           MNRALPE+LHC N+ GNC DTL+VLERQRARLKWQQD   QQ  +Q CF+RADYGG FPP
Sbjct: 2   MNRALPEMLHCLNSTGNCSDTLSVLERQRARLKWQQDMLFQQPLNQSCFSRADYGGGFPP 61

Query: 61  PVAVPPDHHLPGFMGFMSGGGEGGSGGGASLVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 120
           PV+VP DH L GF G+MSGGG  G GGG S VEMVMGAVKPDPGLEDGWSEMGKFDPSLL
Sbjct: 62  PVSVPADH-LTGFPGYMSGGG--GVGGGLSHVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 121

Query: 121 LNATAYELNSSLSRTSSC----PPAVPEKVGSAAGRESFKKRKAEKVQNNNSSSNNNKAS 180
           LN TA ELNSSLSRTSSC     P V EK+GS AGRESFKKRKAEK  N  +++N N   
Sbjct: 122 LNPTACELNSSLSRTSSCLPVVAPTVAEKMGSMAGRESFKKRKAEKAHNTTTTANTNNNK 181

Query: 181 FYNMISLFVTVEEDNNNNSNKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRET 240
                   VTVEED NNNS +  KRIK S+E   SK TDQN TKNN ST TTTTNNNRET
Sbjct: 182 --------VTVEEDENNNSKE--KRIKTSSEGELSKTTDQNGTKNN-STITTTTNNNRET 241

Query: 241 SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK 300
           SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK
Sbjct: 242 SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK 301

Query: 301 ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTANFPA 360
           ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPR DFNVDDLF KEVFPP CT ANFP+
Sbjct: 302 ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNVDDLFNKEVFPPSCTAANFPS 361

Query: 361 IGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFPENFLDSSC 420
           +GG+SSEMTD SSYLQFNPNNQQ+   CGLEMG+NTS V LRR+ISAPVSFPENFLDSSC
Sbjct: 362 VGGMSSEMTDPSSYLQFNPNNQQMDSCCGLEMGINTSHVALRRTISAPVSFPENFLDSSC 421

Query: 421 LTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           LTQFQPSS WDVDLQN+YNVGFDQ RSSNAFSS PYTG
Sbjct: 422 LTQFQPSSGWDVDLQNMYNVGFDQGRSSNAFSSHPYTG 445

BLAST of MC04g0462 vs. ExPASy TrEMBL
Match: A0A1S3BJH6 (transcription factor bHLH49-like OS=Cucumis melo OX=3656 GN=LOC103490373 PE=4 SV=1)

HSP 1 Score: 676 bits (1745), Expect = 4.43e-239
Identity = 372/458 (81.22%), Postives = 390/458 (85.15%), Query Frame = 0

Query: 1   MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFLQQQ-SQGCFNRADYGG-FPP 60
           MNRALPE+LHC NT GNC DTL+VLERQRARLKWQQDQ  QQ  +Q CFNRADYGG FPP
Sbjct: 174 MNRALPEMLHCLNTNGNCSDTLSVLERQRARLKWQQDQLFQQPLNQSCFNRADYGGGFPP 233

Query: 61  PVAVPPDHHLPGFMGFMSGGGEGGSGGGASLVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 120
           PV+VP DH L GF G+MSGGG GG  GG + VEMVMGAVKPDPGLEDGWSEMGKFDPSLL
Sbjct: 234 PVSVPVDH-LTGFPGYMSGGGGGG--GGLAHVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 293

Query: 121 LNATAYELNSSLSRTSSCPPAVP----EKVGSAAGRESFKKRKAEKVQNNNSSSNNNKAS 180
           LNATA ELNSSLSRTSSC P V     EK+GS AGRESFKKRKAEK  N  +++N+N   
Sbjct: 294 LNATACELNSSLSRTSSCLPVVAPTAAEKMGSVAGRESFKKRKAEKAHNTTTTNNSNNK- 353

Query: 181 FYNMISLFVTVEEDNNNNSNKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRET 240
                   VTVEED NN+  K   RIK S+E   SK TDQN TKNN ST TTTTNNNRET
Sbjct: 354 --------VTVEEDENNSKEK---RIKTSSEGELSKTTDQNGTKNN-STITTTTNNNRET 413

Query: 241 SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK 300
           SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK
Sbjct: 414 SADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNK 473

Query: 301 ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTANFPA 360
           ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPR DFNVDDLF KEVFPP CT ANFPA
Sbjct: 474 ITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNVDDLFNKEVFPPSCTAANFPA 533

Query: 361 IGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFPENFLDSSC 420
           IGG+SSEMTD SSYLQFNPNNQQ+   CGLEMG+NTS V LRR+ISAPVSFPENFLDSSC
Sbjct: 534 IGGMSSEMTDPSSYLQFNPNNQQMDSCCGLEMGINTSHVALRRTISAPVSFPENFLDSSC 593

Query: 421 LTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           LTQFQPSS WDVDLQN+YNVGFDQ RSSNAFSSQPYTG
Sbjct: 594 LTQFQPSSGWDVDLQNMYNVGFDQGRSSNAFSSQPYTG 615

BLAST of MC04g0462 vs. ExPASy TrEMBL
Match: A0A6J1C260 (transcription factor bHLH63-like OS=Momordica charantia OX=3673 GN=LOC111007582 PE=4 SV=1)

HSP 1 Score: 662 bits (1708), Expect = 1.21e-237
Identity = 349/359 (97.21%), Postives = 349/359 (97.21%), Query Frame = 0

Query: 94  MGAVKPDPGLEDGWSEMGKFDPSLLLNATAYELNSSLSRTSSCPPAVPEKVGSAAGRESF 153
           MGAVKPDPGLEDGWSEMGKFDPSLLLNATAYELNSSLSRTSSCPPAVPEKVGSAAGRESF
Sbjct: 1   MGAVKPDPGLEDGWSEMGKFDPSLLLNATAYELNSSLSRTSSCPPAVPEKVGSAAGRESF 60

Query: 154 KKRKAEKVQNNNSSSNNNKASFYNMISLFVTVEEDNNNNSNKEIKRIKASAEEGESKATD 213
           KKRKAEKVQNNNSSSNNNK          VTVEEDNNNNSNKEIKRIKASAEEGESKATD
Sbjct: 61  KKRKAEKVQNNNSSSNNNK----------VTVEEDNNNNSNKEIKRIKASAEEGESKATD 120

Query: 214 QNSTKNNNSTTTTTTNNNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERA 273
           QNSTKNNNSTTTTTTNNNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERA
Sbjct: 121 QNSTKNNNSTTTTTTNNNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERA 180

Query: 274 RREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFN 333
           RREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFN
Sbjct: 181 RREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFN 240

Query: 334 VDDLFGKEVFPPPCTTANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDV 393
           VDDLFGKEVFPPPCTTANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDV
Sbjct: 241 VDDLFGKEVFPPPCTTANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDV 300

Query: 394 GLRRSISAPVSFPENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           GLRRSISAPVSFPENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG
Sbjct: 301 GLRRSISAPVSFPENFLDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 349

BLAST of MC04g0462 vs. ExPASy TrEMBL
Match: A0A6J1F684 (transcription factor bHLH63-like OS=Cucurbita moschata OX=3662 GN=LOC111441198 PE=4 SV=1)

HSP 1 Score: 600 bits (1548), Expect = 2.84e-212
Identity = 351/461 (76.14%), Postives = 362/461 (78.52%), Query Frame = 0

Query: 1   MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFLQQQSQGCFNRADYGGFPPPV 60
           MNRALPE+L   NTA NC DTLTVLERQRAR KWQQDQ L QQ Q      DYG      
Sbjct: 1   MNRALPEML---NTAENCADTLTVLERQRARFKWQQDQLLYQQQQPFNGMMDYG-----A 60

Query: 61  AVPPDHHLPGFMGFMSGGGEGGSGGGASLVEMVMGAVKPDPGLEDGWSEMGKFDPSLLLN 120
             PP  HL GF GFMS  G+GG GGG SLVEMVMGAVKPDPGLEDGWS     DPSLLLN
Sbjct: 61  GFPPSDHLTGFPGFMSAAGDGG-GGGLSLVEMVMGAVKPDPGLEDGWS-----DPSLLLN 120

Query: 121 A-TAYELNSSLSRTSSCPPAVPEKVGSAAGRESFKKRKAEKVQNNNSSSNNNKASFYNMI 180
             TAYELNSSLSRTSSCPPAV     +A GRESFKKRKAEKVQ                 
Sbjct: 121 PPTAYELNSSLSRTSSCPPAV--NPAAAPGRESFKKRKAEKVQKPI-------------- 180

Query: 181 SLFVTVEEDNNNNSNKEIKRIKASAEEGE-------SKATDQNSTKNNNST-TTTTTNNN 240
                        +NKE K+IKAS+EE E       SK TDQNSTKNNNST TTTTTNNN
Sbjct: 181 -------------NNKE-KKIKASSEEEEEEEEEELSKTTDQNSTKNNNSTSTTTTTNNN 240

Query: 241 RETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPG 300
           RETSADTSK    ASEV+KPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPG
Sbjct: 241 RETSADTSK----ASEVKKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPG 300

Query: 301 CNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTAN 360
           CNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPR DFNVDDLF KEVFPPPCTTA 
Sbjct: 301 CNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNVDDLFLKEVFPPPCTTAT 360

Query: 361 FPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFPENFLD 420
           F AIGGISSE+TD SSYLQFNPNNQQIVP CGLE+G+NTSDV LRR+ISAPVSFPENFLD
Sbjct: 361 FAAIGGISSEITDPSSYLQFNPNNQQIVPCCGLELGINTSDVALRRTISAPVSFPENFLD 413

Query: 421 SSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           SSCLTQFQ SS WDVDLQNLYNVGFDQ RSSNAFSSQPYTG
Sbjct: 421 SSCLTQFQGSSGWDVDLQNLYNVGFDQGRSSNAFSSQPYTG 413

BLAST of MC04g0462 vs. ExPASy TrEMBL
Match: A0A6J1I0A0 (transcription factor bHLH63-like OS=Cucurbita maxima OX=3661 GN=LOC111469692 PE=4 SV=1)

HSP 1 Score: 597 bits (1538), Expect = 1.01e-210
Identity = 351/463 (75.81%), Postives = 360/463 (77.75%), Query Frame = 0

Query: 1   MNRALPEILHCFNTAGNCPDTLTVLERQRARLKWQQDQFL--QQQSQGCFNRADYGGFPP 60
           MNRALPE+    NTA NC DTLTVLERQRAR KWQQDQ L  QQQ Q      DYG    
Sbjct: 1   MNRALPEMS---NTAENCVDTLTVLERQRARFKWQQDQLLYQQQQLQSFNGMMDYG---- 60

Query: 61  PVAVPPDHHLPGFMGFMSGGGEGGSGGGASLVEMVMGAVKPDPGLEDGWSEMGKFDPSLL 120
               PP  HL GF GFMS  G GG GG  SLVEMVMGAVKPDPGLEDGWS     DPSLL
Sbjct: 61  -AGFPPSDHLTGFPGFMSAAGAGGDGG-LSLVEMVMGAVKPDPGLEDGWS-----DPSLL 120

Query: 121 LNA-TAYELNSSLSRTSSCPPAVPEKVGSAAGRESFKKRKAEKVQNNNSSSNNNKASFYN 180
           LN  TAYELNSSLSRTSSCPPAV     +AAGRESFKKRKAEKVQN              
Sbjct: 121 LNPPTAYELNSSLSRTSSCPPAV--NPAAAAGRESFKKRKAEKVQNPI------------ 180

Query: 181 MISLFVTVEEDNNNNSNKEIKRIKASAEEGE--------SKATDQNSTKNNNSTTTTTTN 240
                          +NKE KRIKAS+EE +        SK TDQNSTKNNNST TTTTN
Sbjct: 181 ---------------NNKE-KRIKASSEEEKEEEEEEELSKTTDQNSTKNNNSTITTTTN 240

Query: 241 NNRETSADTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLV 300
           NNRETSADTSK    ASEV+KPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLV
Sbjct: 241 NNRETSADTSK----ASEVKKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLV 300

Query: 301 PGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTT 360
           PGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPR DFNVDDLF KEVFPPPCTT
Sbjct: 301 PGCNKITGKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRLDFNVDDLFLKEVFPPPCTT 360

Query: 361 ANFPAIGGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSFPENF 420
           ANF AIGGISSE+TD SSY QFN NNQQIVP CGLEMG+NTSDV LRR+ISAPVSFPENF
Sbjct: 361 ANFAAIGGISSEITDPSSYFQFNRNNQQIVPCCGLEMGINTSDVALRRTISAPVSFPENF 415

Query: 421 LDSSCLTQFQPSSAWDVDLQNLYNVGFDQARSSNAFSSQPYTG 452
           LDSSCLTQFQ SS WDVDLQNLYNVGFDQ RSSNAFSSQPYTG
Sbjct: 421 LDSSCLTQFQGSSGWDVDLQNLYNVGFDQGRSSNAFSSQPYTG 415

BLAST of MC04g0462 vs. TAIR 10
Match: AT4G34530.1 (cryptochrome-interacting basic-helix-loop-helix 1 )

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-45
Identity = 133/271 (49.08%), Postives = 153/271 (56.46%), Query Frame = 0

Query: 182 FVTVEEDNNNNSNKEIKRIKASAEEGE---SKATDQN--STKNNNSTTTTTTNNNRETSA 241
           F T  +D N    K         EEGE   SK T+QN  STK+               S 
Sbjct: 96  FDTETKDCNEKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSN 155

Query: 242 DTSKENSKASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKIT 301
           D+SK      E++K DYIHVRARRGQATDSHS+AER RREKISERMK+LQDLVPGC+KIT
Sbjct: 156 DSSK---VTKELEKTDYIHVRARRGQATDSHSIAERVRREKISERMKFLQDLVPGCDKIT 215

Query: 302 GKAGMLDEIINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVFPPPCTTANFPAI- 361
           GKAGMLDEIINYVQSLQRQ+EFLSMKLA VNPR DF++DD+F KEV   P T    P + 
Sbjct: 216 GKAGMLDEIINYVQSLQRQIEFLSMKLAIVNPRPDFDMDDIFAKEVASTPMTVVPSPEMV 275

Query: 362 ----------GGISSEMTDHSSYLQFNPNNQQIVPGCGLEMGMNTSDVGLRRSISAPVSF 421
                      G SSEM  +S YL  NP  Q           +NTS        S P+  
Sbjct: 276 LSGYSHEMVHSGYSSEMV-NSGYLHVNPMQQ-----------VNTS--------SDPL-- 334

Query: 422 PENFLDSSCLTQFQPSSAWDVDLQNLY-NVG 436
                  SC    +  S WD  +QNLY N+G
Sbjct: 336 -------SCFNNGEAPSMWDSHVQNLYGNLG 334

BLAST of MC04g0462 vs. TAIR 10
Match: AT2G18300.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 7.2e-38
Identity = 93/151 (61.59%), Postives = 113/151 (74.83%), Query Frame = 0

Query: 194 NKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRETSADTSKENSK-ASEVQKPD 253
           NK    +K + EE +++   +   +  +S    +   N E S+DTSKE SK ASE QK D
Sbjct: 123 NKRKPEVK-TREEQKTEKKIKVEAETESSMKGKSNMGNTEASSDTSKETSKGASENQKLD 182

Query: 254 YIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSL 313
           YIHVRARRGQATD HSLAERARREKIS++MKYLQD+VPGCNK+TGKAGMLDEIINYVQ L
Sbjct: 183 YIHVRARRGQATDRHSLAERARREKISKKMKYLQDIVPGCNKVTGKAGMLDEIINYVQCL 242

Query: 314 QRQVEFLSMKLAAVNPRFDFNVDDLFGKEVF 344
           QRQVEFLSMKLA +NP  +  V+D+  K+ +
Sbjct: 243 QRQVEFLSMKLAVLNPELELAVEDVSVKQAY 272

BLAST of MC04g0462 vs. TAIR 10
Match: AT2G18300.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 1.6e-37
Identity = 93/149 (62.42%), Postives = 112/149 (75.17%), Query Frame = 0

Query: 194 NKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRETSADTSKENSK-ASEVQKPD 253
           NK    +K + EE +++   +   +  +S    +   N E S+DTSKE SK ASE QK D
Sbjct: 123 NKRKPEVK-TREEQKTEKKIKVEAETESSMKGKSNMGNTEASSDTSKETSKGASENQKLD 182

Query: 254 YIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSL 313
           YIHVRARRGQATD HSLAERARREKIS++MKYLQD+VPGCNK+TGKAGMLDEIINYVQ L
Sbjct: 183 YIHVRARRGQATDRHSLAERARREKISKKMKYLQDIVPGCNKVTGKAGMLDEIINYVQCL 242

Query: 314 QRQVEFLSMKLAAVNPRFDFNVDDLFGKE 342
           QRQVEFLSMKLA +NP  +  V+D+  K+
Sbjct: 243 QRQVEFLSMKLAVLNPELELAVEDVSVKQ 270

BLAST of MC04g0462 vs. TAIR 10
Match: AT2G18300.3 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 1.6e-37
Identity = 93/149 (62.42%), Postives = 112/149 (75.17%), Query Frame = 0

Query: 194 NKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRETSADTSKENSK-ASEVQKPD 253
           NK    +K + EE +++   +   +  +S    +   N E S+DTSKE SK ASE QK D
Sbjct: 123 NKRKPEVK-TREEQKTEKKIKVEAETESSMKGKSNMGNTEASSDTSKETSKGASENQKLD 182

Query: 254 YIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSL 313
           YIHVRARRGQATD HSLAERARREKIS++MKYLQD+VPGCNK+TGKAGMLDEIINYVQ L
Sbjct: 183 YIHVRARRGQATDRHSLAERARREKISKKMKYLQDIVPGCNKVTGKAGMLDEIINYVQCL 242

Query: 314 QRQVEFLSMKLAAVNPRFDFNVDDLFGKE 342
           QRQVEFLSMKLA +NP  +  V+D+  K+
Sbjct: 243 QRQVEFLSMKLAVLNPELELAVEDVSVKQ 270

BLAST of MC04g0462 vs. TAIR 10
Match: AT4G36540.2 (BR enhanced expression 2 )

HSP 1 Score: 151.4 bits (381), Expect = 1.8e-36
Identity = 85/159 (53.46%), Postives = 113/159 (71.07%), Query Frame = 0

Query: 185 VEEDNNNNSNKEIKRIKASAEEGESKATDQNSTKNNNSTTTTTTNNNRETSADTSKENSK 244
           +E   NN  ++ I        EG+++  ++   K  + T  +    +  ++ +TS     
Sbjct: 76  MEPVKNNGHSRAITLQNKRKPEGKTEKREKKKIKAEDETEPSMKGKSNMSNTETS----- 135

Query: 245 ASEVQKPDYIHVRARRGQATDSHSLAERARREKISERMKYLQDLVPGCNKITGKAGMLDE 304
            SE+QKPDYIHVRARRG+ATD HSLAERARREKIS++MK LQD+VPGCNK+TGKAGMLDE
Sbjct: 136 -SEIQKPDYIHVRARRGEATDRHSLAERARREKISKKMKCLQDIVPGCNKVTGKAGMLDE 195

Query: 305 IINYVQSLQRQVEFLSMKLAAVNPRFDFNVDDLFGKEVF 344
           IINYVQSLQ+QVEFLSMKL+ +NP  + ++DDL  K+ +
Sbjct: 196 IINYVQSLQQQVEFLSMKLSVINPELECHIDDLSAKQAY 228

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GY611.7e-4449.08Transcription factor bHLH63 OS=Arabidopsis thaliana OX=3702 GN=BHLH63 PE=1 SV=1[more]
Q9ZPW32.3e-3662.42Transcription factor HBI1 OS=Arabidopsis thaliana OX=3702 GN=HBI1 PE=1 SV=3[more]
Q93VJ45.6e-3554.14Transcription factor BEE 2 OS=Arabidopsis thaliana OX=3702 GN=BEE2 PE=1 SV=1[more]
Q9CAA95.6e-3541.80Transcription factor bHLH49 OS=Arabidopsis thaliana OX=3702 GN=BHLH49 PE=1 SV=1[more]
Q9SRT22.1e-3437.21Transcription factor bHLH62 OS=Arabidopsis thaliana OX=3702 GN=BHLH62 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038888827.11.94e-24682.72transcription factor bHLH63-like [Benincasa hispida][more]
XP_011658545.13.91e-23980.35transcription factor bHLH63 [Cucumis sativus] >KGN43145.1 hypothetical protein C... [more]
XP_008448085.19.15e-23981.22PREDICTED: transcription factor bHLH49-like [Cucumis melo][more]
XP_022135684.12.49e-23797.21transcription factor bHLH63-like [Momordica charantia][more]
XP_023529229.17.71e-21476.39transcription factor bHLH63-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0K0C81.89e-23980.35BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G000520 PE=4 S... [more]
A0A1S3BJH64.43e-23981.22transcription factor bHLH49-like OS=Cucumis melo OX=3656 GN=LOC103490373 PE=4 SV... [more]
A0A6J1C2601.21e-23797.21transcription factor bHLH63-like OS=Momordica charantia OX=3673 GN=LOC111007582 ... [more]
A0A6J1F6842.84e-21276.14transcription factor bHLH63-like OS=Cucurbita moschata OX=3662 GN=LOC111441198 P... [more]
A0A6J1I0A01.01e-21075.81transcription factor bHLH63-like OS=Cucurbita maxima OX=3661 GN=LOC111469692 PE=... [more]
Match NameE-valueIdentityDescription
AT4G34530.11.2e-4549.08cryptochrome-interacting basic-helix-loop-helix 1 [more]
AT2G18300.17.2e-3861.59basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G18300.21.6e-3762.42basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G18300.31.6e-3762.42basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT4G36540.21.8e-3653.46BR enhanced expression 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 268..318
e-value: 2.3E-9
score: 47.1
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 266..313
e-value: 1.2E-6
score: 28.4
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 262..312
score: 15.579952
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 259..331
e-value: 7.8E-15
score: 56.8
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 260..330
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 203..251
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 237..251
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 130..172
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..236
NoneNo IPR availablePANTHERPTHR12565:SF395TRANSCRIPTION FACTOR BHLH63-LIKEcoord: 20..439
NoneNo IPR availableCDDcd18919bHLH_AtBPE_likecoord: 252..337
e-value: 2.6456E-66
score: 204.983
IPR024097Basic helix-loop-helix leucine zipper transcription factorPANTHERPTHR12565STEROL REGULATORY ELEMENT-BINDING PROTEINcoord: 20..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0462.1MC04g0462.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0046983 protein dimerization activity