MC04g0412 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g0412 vs. ExPASy Swiss-Prot
Match: P45434 (Translocon-associated protein subunit alpha OS=Arabidopsis thaliana OX=3702 GN=At2g21160 PE=2 SV=3)

HSP 1 Score: 271.6 bits (693), Expect = 9.5e-72
Identity = 149/261 (57.09%), Postives = 199/261 (76.25%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETS---EVVEEASDLGIVGEEDSQDFGEVN 60
           M    V FLALLLLASP+ +VA CQSD++  +S   +VV E +D  +  EED  D  ++N
Sbjct: 1   MMNLRVLFLALLLLASPLLQVARCQSDAEDHSSLVDDVVGENTDDAV--EEDDHDL-DMN 60

Query: 61  FAPAPGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLI 120
            +  PGV+TVCVFPKNSA++VPAGEETELL G+KN+G++ + ++ I+ASVH  +DH +L+
Sbjct: 61  LSSFPGVETVCVFPKNSAKLVPAGEETELLVGLKNEGKTRVGVMGIRASVHLPYDHKLLV 120

Query: 121 QNLTGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTI 180
           QNLT    +NA++  S QATFPYIFAVS++LQPG FDLVG I Y+V    YQSVF+NGTI
Sbjct: 121 QNLTMLRLNNASIPTSLQATFPYIFAVSQYLQPGAFDLVGYIIYDVEGKPYQSVFYNGTI 180

Query: 181 EVVEASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTS 240
           EVVE+ G++S ESVFL+TLGI LL+LLGLW + Q+ Q+L+KKTK+  SKVEVGT++ + S
Sbjct: 181 EVVESGGLLSGESVFLLTLGIGLLLLLGLWAYSQV-QRLTKKTKK-VSKVEVGTRSTEAS 240

Query: 241 MDEWLQGTAYNQSQSNKSKKK 259
           +DEWL+GT   ++ S K+K K
Sbjct: 241 LDEWLEGTTLAKTSSGKTKNK 256

BLAST of MC04g0412 vs. ExPASy Swiss-Prot
Match: Q54R45 (Translocon-associated protein subunit alpha OS=Dictyostelium discoideum OX=44689 GN=ssr1 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.3e-16
Identity = 60/202 (29.70%), Postives = 98/202 (48.51%), Query Frame = 0

Query: 56  VNFAPAPGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHM 115
           V   P   V    +FP N  +   AG   E+L G  N+ + +LNI  I AS+++  D  +
Sbjct: 31  VQATPTSDVSFSYIFPDNQDKNFAAGSVVEVLVGFTNNADKALNITHIFASLNHPQDFSV 90

Query: 116 LIQNLTGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNG 175
            IQN T   F    V     AT  Y F  S++L+P  F L+ ++ Y+     +   FFN 
Sbjct: 91  FIQNYTRGDF-GIAVEKGHHATLAYRFVPSEYLEPREFGLLISLEYQDGAQNFTQTFFNS 150

Query: 176 TIEVVEASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVD 235
           TI + E      ++S FL+ LG+  +  +G  ++G++ ++   +T    +K  V  +T D
Sbjct: 151 TINITEKETSFDMDSFFLILLGLGFVGGIGYIVYGKMPKQKKVRTVSKVNKNAVRVETED 210

Query: 236 TSMDEWLQGTAYNQSQSNKSKK 258
            +  EWL GT+   S+    +K
Sbjct: 211 ETA-EWLSGTSAASSKVKSVQK 230

BLAST of MC04g0412 vs. ExPASy Swiss-Prot
Match: A6QLP7 (Translocon-associated protein subunit alpha OS=Bos taurus OX=9913 GN=SSR1 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.8e-15
Identity = 80/258 (31.01%), Postives = 118/258 (45.74%), Query Frame = 0

Query: 6   VFFLALLLLASPVFRVATCQS-DSDSETSE--VVEEASDLGIVGEEDSQDFGE------V 65
           VF   LLL   P   +A  Q    D ET E  ++E+  D   V E++  D  E      V
Sbjct: 14  VFPATLLLRVGPGGSLAVAQDLTEDEETVEDSIIEDEDDEAEVEEDEPTDLAEDKEEDDV 73

Query: 66  NFAP--APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHH 125
           +  P  +P  DT  +F K      PA    + L G  N G     + ++ AS  Y  D+ 
Sbjct: 74  SGEPEASPSADTTILFVKGED--FPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQDYQ 133

Query: 126 MLIQNLTGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFY-EVNQNAYQSVFF 185
             IQN T     N  V P  QATF Y F  ++ +    F LV  + Y ++N N +Q   F
Sbjct: 134 FYIQNFTALPL-NTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVF 193

Query: 186 NGTIEVVEASGVISIESVFLVTL--GIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGT 245
           N T+ ++E    +  E++F+     G+ LLV++GL  H  ++   S+K KR   KVE+GT
Sbjct: 194 NQTVTIIEREDGLDGETIFMYMFLAGLGLLVVVGL--HQLLE---SRKRKRPIQKVEMGT 253

Query: 246 KTVDTSMDEWLQGTAYNQ 250
            + +     W+     NQ
Sbjct: 254 SSQNDVDMSWIPQETLNQ 263

BLAST of MC04g0412 vs. ExPASy Swiss-Prot
Match: Q9CY50 (Translocon-associated protein subunit alpha OS=Mus musculus OX=10090 GN=Ssr1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 8.2e-15
Identity = 77/257 (29.96%), Postives = 117/257 (45.53%), Query Frame = 0

Query: 7   FFLALLLLASPVFRVATCQSDSDSE---TSEVVEEASDLGIVGEEDSQDFGE------VN 66
           F  A+LL   P   +A  Q  ++ E      ++E+  D   V E++  D  E      V+
Sbjct: 15  FPAAVLLRGGPGGSLALAQDPTEDEEIVEDSIIEDEDDEAEVEEDEPTDLAEDKEEEDVS 74

Query: 67  FAP--APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHM 126
             P  +P  DT  +F K      PA    + L G  N G     + ++ AS  Y  D+  
Sbjct: 75  SEPEASPSADTTILFVKGED--FPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQDYQF 134

Query: 127 LIQNLTGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFY-EVNQNAYQSVFFN 186
            IQN T     N  V P  QATF Y F  ++ +    F LV  + Y ++N N +Q   FN
Sbjct: 135 YIQNFTALPL-NTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFN 194

Query: 187 GTIEVVEASGVISIESVFLVTL--GIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTK 246
            T+ V+E    +  E++F+     G+ LLV++GL  H  ++   S+K KR   KVE+GT 
Sbjct: 195 QTVTVIEREDGLDGETIFMYMFLAGLGLLVVVGL--HQLLE---SRKRKRPIQKVEMGTS 254

Query: 247 TVDTSMDEWLQGTAYNQ 250
           + +     W+     NQ
Sbjct: 255 SQNDVDMSWIPQETLNQ 263

BLAST of MC04g0412 vs. ExPASy Swiss-Prot
Match: P16967 (Translocon-associated protein subunit alpha OS=Canis lupus familiaris OX=9615 GN=SSR1 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.8e-14
Identity = 81/264 (30.68%), Postives = 118/264 (44.70%), Query Frame = 0

Query: 9   LALLLLASPV----------FRVATCQSDSDSETSE--VVEEASDLGIVGEEDSQDFGE- 68
           L LLLLA P             VA      D ET E  ++E+  D   V E++  D  E 
Sbjct: 8   LLLLLLAFPAAVLLRGGPGGSLVAAQDLTEDEETVEDSIIEDEDDEAEVEEDEPTDLAED 67

Query: 69  -----VNFAP--APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVH 128
                V+  P  +P  DT  +F K      PA    + L G  N G     + ++ AS  
Sbjct: 68  KEEEDVSGEPEASPSADTTILFVKGED--FPANNIVKFLVGFTNKGTEDFIVESLDASFR 127

Query: 129 YTFDHHMLIQNLTGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFY-EVNQNA 188
           Y  D+   IQN T     N  V P  QATF Y F  ++ +    F LV  + Y ++N N 
Sbjct: 128 YPQDYQFYIQNFTALPL-NTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNV 187

Query: 189 YQSVFFNGTIEVVEASGVISIESVFLVTL--GIALLVLLGLWIHGQIQQKLSKKTKRAAS 248
           +Q   FN T+ ++E    +  E++F+     G+ LLV++GL  H  ++   S+K KR   
Sbjct: 188 FQDAVFNQTVTIIEREDGLDGETIFMYMFLAGLGLLVVVGL--HQLLE---SRKRKRPIQ 247

Query: 249 KVEVGTKTVDTSMDEWLQGTAYNQ 250
           KVE+GT + +     W+     NQ
Sbjct: 248 KVEMGTSSQNDVDMSWIPQETLNQ 263

BLAST of MC04g0412 vs. NCBI nr
Match: XP_022135777.1 (translocon-associated protein subunit alpha-like [Momordica charantia])

HSP 1 Score: 491 bits (1264), Expect = 1.26e-174
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP
Sbjct: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV
Sbjct: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE
Sbjct: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTAYNQSQSNKSKKKN
Sbjct: 241 WLQGTAYNQSQSNKSKKKN 259

BLAST of MC04g0412 vs. NCBI nr
Match: XP_022952473.1 (translocon-associated protein subunit alpha-like [Cucurbita moschata])

HSP 1 Score: 437 bits (1124), Expect = 2.62e-153
Identity = 230/259 (88.80%), Postives = 245/259 (94.59%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           MK F VFFLALLLLASP  + ATCQSDSD E+SEVVEEASDLG+VGEEDSQDFGEVNF P
Sbjct: 1   MKTFRVFFLALLLLASPFLQAATCQSDSDVESSEVVEEASDLGVVGEEDSQDFGEVNFGP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLN+IAIKASVHYTFDH MLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNVIAIKASVHYTFDHRMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATV PSTQA++PYIFAVSKFLQPGNFDLVGTIFYE++Q AYQSVFFNGTIEVV
Sbjct: 121 TGQVFHNATVPPSTQASYPYIFAVSKFLQPGNFDLVGTIFYEIDQTAYQSVFFNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASG+IS+E+VFLVTLGIALLVLLGLWIHGQIQQKLSKK KR ASKVEVGTKT+DTSMDE
Sbjct: 181 EASGLISMETVFLVTLGIALLVLLGLWIHGQIQQKLSKKPKRVASKVEVGTKTIDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTA+ +S S+KSKKKN
Sbjct: 241 WLQGTAFTRS-SSKSKKKN 258

BLAST of MC04g0412 vs. NCBI nr
Match: KAG6572098.1 (Translocon-associated protein subunit alpha, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011750.1 Translocon-associated protein subunit alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 436 bits (1122), Expect = 5.29e-153
Identity = 229/259 (88.42%), Postives = 244/259 (94.21%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           MK F VFFLALLLLASP  + ATCQSDSD E+SE VEEASDLG+VGEEDSQDFGEVNF P
Sbjct: 1   MKTFRVFFLALLLLASPFLQAATCQSDSDGESSEAVEEASDLGVVGEEDSQDFGEVNFGP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLN+IAIKASVHYTFDH MLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNVIAIKASVHYTFDHRMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATV PSTQA++PYIFAVSKFLQPGNFDLVGTIFYE++Q AYQSVFFNGTIEVV
Sbjct: 121 TGQVFHNATVPPSTQASYPYIFAVSKFLQPGNFDLVGTIFYEIDQTAYQSVFFNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASG+IS+E+VFLVTLGIALLVLLGLWIHGQIQQKLSKK KR ASKVEVGTKT+DTSMDE
Sbjct: 181 EASGLISMETVFLVTLGIALLVLLGLWIHGQIQQKLSKKPKRVASKVEVGTKTIDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTA+ +S S+KSKKKN
Sbjct: 241 WLQGTAFTRS-SSKSKKKN 258

BLAST of MC04g0412 vs. NCBI nr
Match: XP_038886848.1 (translocon-associated protein subunit alpha-like [Benincasa hispida])

HSP 1 Score: 436 bits (1121), Expect = 7.79e-153
Identity = 228/259 (88.03%), Postives = 241/259 (93.05%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           M  F VFFLA LLLASP  + ATCQSDSD E+SE VEE SDLGIVGEEDSQDFGEVNF P
Sbjct: 1   MNAFRVFFLAFLLLASPFLQAATCQSDSDVESSEAVEEVSDLGIVGEEDSQDFGEVNFGP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKAS+HYTFDH +LIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASIHYTFDHRLLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATV PSTQAT+PY+FAVSKFLQPGNFDLVGTIFYE++QNAYQS+F+NGTIEVV
Sbjct: 121 TGQVFHNATVPPSTQATYPYMFAVSKFLQPGNFDLVGTIFYEIDQNAYQSIFYNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASG+ISIESVFLVTLGIALLVLL LWIHGQIQQKLSKK KR ASKVEVGTKTVDTSMDE
Sbjct: 181 EASGIISIESVFLVTLGIALLVLLVLWIHGQIQQKLSKKPKRTASKVEVGTKTVDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTAY +S S+KSKKKN
Sbjct: 241 WLQGTAYTKSGSSKSKKKN 259

BLAST of MC04g0412 vs. NCBI nr
Match: XP_023553718.1 (translocon-associated protein subunit alpha-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 434 bits (1115), Expect = 6.17e-152
Identity = 227/259 (87.64%), Postives = 243/259 (93.82%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           MK F VFFLALLLLASP  + ATCQSDSD E+SE VEEASD+GIVGEEDSQDFGEVNF P
Sbjct: 1   MKTFRVFFLALLLLASPFLQAATCQSDSDGESSEAVEEASDIGIVGEEDSQDFGEVNFGP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLN+IAIKAS+HYTFDH MLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNVIAIKASIHYTFDHRMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATV PS QA++PYIFAVSKFLQPGNFDLVGTIFYE++Q AYQSVFFNGTIEVV
Sbjct: 121 TGQVFHNATVPPSAQASYPYIFAVSKFLQPGNFDLVGTIFYEIDQTAYQSVFFNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASG+IS+E+VFLVTLGIALLVLLGLWIHGQIQQKLSKK KR ASKVEVGTKT+DTSMDE
Sbjct: 181 EASGLISMETVFLVTLGIALLVLLGLWIHGQIQQKLSKKPKRVASKVEVGTKTIDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTA+ +S S+KSKKKN
Sbjct: 241 WLQGTAFTRS-SSKSKKKN 258

BLAST of MC04g0412 vs. ExPASy TrEMBL
Match: A0A6J1C3Q7 (Signal sequence receptor subunit alpha OS=Momordica charantia OX=3673 GN=LOC111007656 PE=3 SV=1)

HSP 1 Score: 491 bits (1264), Expect = 6.09e-175
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP
Sbjct: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV
Sbjct: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE
Sbjct: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTAYNQSQSNKSKKKN
Sbjct: 241 WLQGTAYNQSQSNKSKKKN 259

BLAST of MC04g0412 vs. ExPASy TrEMBL
Match: A0A6J1GKP1 (Signal sequence receptor subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111455149 PE=3 SV=1)

HSP 1 Score: 437 bits (1124), Expect = 1.27e-153
Identity = 230/259 (88.80%), Postives = 245/259 (94.59%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           MK F VFFLALLLLASP  + ATCQSDSD E+SEVVEEASDLG+VGEEDSQDFGEVNF P
Sbjct: 1   MKTFRVFFLALLLLASPFLQAATCQSDSDVESSEVVEEASDLGVVGEEDSQDFGEVNFGP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLN+IAIKASVHYTFDH MLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNVIAIKASVHYTFDHRMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATV PSTQA++PYIFAVSKFLQPGNFDLVGTIFYE++Q AYQSVFFNGTIEVV
Sbjct: 121 TGQVFHNATVPPSTQASYPYIFAVSKFLQPGNFDLVGTIFYEIDQTAYQSVFFNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASG+IS+E+VFLVTLGIALLVLLGLWIHGQIQQKLSKK KR ASKVEVGTKT+DTSMDE
Sbjct: 181 EASGLISMETVFLVTLGIALLVLLGLWIHGQIQQKLSKKPKRVASKVEVGTKTIDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTA+ +S S+KSKKKN
Sbjct: 241 WLQGTAFTRS-SSKSKKKN 258

BLAST of MC04g0412 vs. ExPASy TrEMBL
Match: A0A6J1I2T7 (Signal sequence receptor subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111468485 PE=3 SV=1)

HSP 1 Score: 433 bits (1113), Expect = 6.02e-152
Identity = 229/259 (88.42%), Postives = 243/259 (93.82%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           MK F VFFLALLLLASP  + AT QSDSD E+SE VEEASDLG+VGEEDSQDFGEVNF P
Sbjct: 1   MKTFRVFFLALLLLASPFLQAATSQSDSDGESSEAVEEASDLGVVGEEDSQDFGEVNFGP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDH MLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHRMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATV PSTQA++PYIFAVSKFLQPGNFDLVGTIFYE++Q AYQSVFFNGTIEVV
Sbjct: 121 TGQVFHNATVPPSTQASYPYIFAVSKFLQPGNFDLVGTIFYEIDQTAYQSVFFNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASG+IS+E+VFLVTLGIALLVLLGLWIHGQIQQKLSKK KR ASKVEVGTKT+DTSMDE
Sbjct: 181 EASGLISMETVFLVTLGIALLVLLGLWIHGQIQQKLSKKPKRVASKVEVGTKTIDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTA+ +S S+KSKKKN
Sbjct: 241 WLQGTAFTRS-SSKSKKKN 258

BLAST of MC04g0412 vs. ExPASy TrEMBL
Match: A0A5D3CLZ0 (Signal sequence receptor subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00850 PE=3 SV=1)

HSP 1 Score: 430 bits (1105), Expect = 1.03e-150
Identity = 223/259 (86.10%), Postives = 240/259 (92.66%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           M  F VFFLALLLLASP  + ATCQ+DSD E SE VEE SDLGIVG+EDSQDFGEVNF P
Sbjct: 1   MNTFRVFFLALLLLASPFLKAATCQADSDVEASEAVEEVSDLGIVGDEDSQDFGEVNFGP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEET+LLAGVKNDGESSLNIIAIKAS+HYTFDH MLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETQLLAGVKNDGESSLNIIAIKASIHYTFDHRMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATV PS QA++PYIFAVSK+LQPGNFDLVGTIFYE++QNAYQS+F+NGTIEVV
Sbjct: 121 TGQVFHNATVPPSAQASYPYIFAVSKYLQPGNFDLVGTIFYEIDQNAYQSIFYNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASG+IS+ESVFLV+LGIALLVLL LWIHGQIQQKLSKK KRAASKVEVGTKT+DTSMDE
Sbjct: 181 EASGIISMESVFLVSLGIALLVLLVLWIHGQIQQKLSKKPKRAASKVEVGTKTIDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTAY +S SNK KKKN
Sbjct: 241 WLQGTAYTKSGSNKLKKKN 259

BLAST of MC04g0412 vs. ExPASy TrEMBL
Match: A0A0A0K182 (Signal sequence receptor subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_7G009720 PE=3 SV=1)

HSP 1 Score: 429 bits (1104), Expect = 1.47e-150
Identity = 223/259 (86.10%), Postives = 242/259 (93.44%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAP 60
           M  F +FFLALLLLASP  + ATCQSDSD E+SE VE+ SDLGIVG+EDSQDFGEVNF P
Sbjct: 1   MNSFRLFFLALLLLASPFLKAATCQSDSDVESSEAVEDVSDLGIVGDEDSQDFGEVNFGP 60

Query: 61  APGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNL 120
           APGVDTVCVFPKNSARIVPAGEET+LLAGVKN+GESSLNIIAIKAS+HYTFDH MLIQNL
Sbjct: 61  APGVDTVCVFPKNSARIVPAGEETQLLAGVKNNGESSLNIIAIKASIHYTFDHRMLIQNL 120

Query: 121 TGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVV 180
           TGQVFHNATV PSTQA++PYIFAVSK+LQPGNFDLVGTIFYE++QNAYQS+F+NGTIEVV
Sbjct: 121 TGQVFHNATVTPSTQASYPYIFAVSKYLQPGNFDLVGTIFYEIDQNAYQSIFYNGTIEVV 180

Query: 181 EASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDE 240
           EASG+IS+ESVFLV+LGIALLVLL LWIHGQIQQKLSKK KRAASKVEVGTKTVDTSMDE
Sbjct: 181 EASGIISMESVFLVSLGIALLVLLVLWIHGQIQQKLSKKPKRAASKVEVGTKTVDTSMDE 240

Query: 241 WLQGTAYNQSQSNKSKKKN 259
           WLQGTAY +S SNK KKKN
Sbjct: 241 WLQGTAYTKSGSNKLKKKN 259

BLAST of MC04g0412 vs. TAIR 10
Match: AT2G21160.1 (Translocon-associated protein (TRAP), alpha subunit )

HSP 1 Score: 271.6 bits (693), Expect = 6.7e-73
Identity = 149/261 (57.09%), Postives = 199/261 (76.25%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETS---EVVEEASDLGIVGEEDSQDFGEVN 60
           M    V FLALLLLASP+ +VA CQSD++  +S   +VV E +D  +  EED  D  ++N
Sbjct: 1   MMNLRVLFLALLLLASPLLQVARCQSDAEDHSSLVDDVVGENTDDAV--EEDDHDL-DMN 60

Query: 61  FAPAPGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLI 120
            +  PGV+TVCVFPKNSA++VPAGEETELL G+KN+G++ + ++ I+ASVH  +DH +L+
Sbjct: 61  LSSFPGVETVCVFPKNSAKLVPAGEETELLVGLKNEGKTRVGVMGIRASVHLPYDHKLLV 120

Query: 121 QNLTGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTI 180
           QNLT    +NA++  S QATFPYIFAVS++LQPG FDLVG I Y+V    YQSVF+NGTI
Sbjct: 121 QNLTMLRLNNASIPTSLQATFPYIFAVSQYLQPGAFDLVGYIIYDVEGKPYQSVFYNGTI 180

Query: 181 EVVEASGVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTS 240
           EVVE+ G++S ESVFL+TLGI LL+LLGLW + Q+ Q+L+KKTK+  SKVEVGT++ + S
Sbjct: 181 EVVESGGLLSGESVFLLTLGIGLLLLLGLWAYSQV-QRLTKKTKK-VSKVEVGTRSTEAS 240

Query: 241 MDEWLQGTAYNQSQSNKSKKK 259
           +DEWL+GT   ++ S K+K K
Sbjct: 241 LDEWLEGTTLAKTSSGKTKNK 256

BLAST of MC04g0412 vs. TAIR 10
Match: AT2G16595.1 (Translocon-associated protein (TRAP), alpha subunit )

HSP 1 Score: 234.2 bits (596), Expect = 1.2e-61
Identity = 135/255 (52.94%), Postives = 175/255 (68.63%), Query Frame = 0

Query: 4   FSVFFLALLLLASPVFRVATCQSDSDSETSEVVEEASDLGIVGEEDSQDFGEVNFAPAPG 63
           F + F A LLL SP  +VA CQSDS+    EV    SD+G   E D+++   +    APG
Sbjct: 5   FRLLFFAFLLLVSPFLKVALCQSDSEDSGFEV----SDVG-EEESDAEEVFGLELTSAPG 64

Query: 64  VDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLIQNLTGQ 123
           ++T+CVFPKNSA+I+ AG ETELL G+KNDG+S+++I+A+K S+H  FD +  +QNLT  
Sbjct: 65  IETLCVFPKNSAKIIKAGVETELLVGMKNDGQSNVDIVAVKGSLHLPFDQN-AVQNLTAL 124

Query: 124 VFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTIEVVEAS 183
            F NA+V  S QAT PY FAVSKFLQ G FD VGTI YE++   YQS+F+NGTIEVVE  
Sbjct: 125 SFSNASVPTSAQATLPYAFAVSKFLQAGAFDFVGTIIYEIDGKPYQSIFYNGTIEVVEDG 184

Query: 184 GVISIESVFLVTLGIALLVLLGLWIHGQIQQKLSKKTKRAASKVEVGTKTVDTSMDEWLQ 243
            +  +ESVFL  L I ++  L ++I   + + ++KKTKR A KVEVGT T D S DEWL+
Sbjct: 185 PLFRMESVFLSGLLIFVIAFLAIYIQ-NVLKHMTKKTKR-APKVEVGTATKDASHDEWLE 244

Query: 244 GTAYNQSQSNKSKKK 259
           GT+Y QS S   KKK
Sbjct: 245 GTSYTQSSSKSKKKK 251

BLAST of MC04g0412 vs. TAIR 10
Match: AT2G21160.2 (Translocon-associated protein (TRAP), alpha subunit )

HSP 1 Score: 201.1 bits (510), Expect = 1.1e-51
Identity = 107/187 (57.22%), Postives = 140/187 (74.87%), Query Frame = 0

Query: 1   MKPFSVFFLALLLLASPVFRVATCQSDSDSETS---EVVEEASDLGIVGEEDSQDFGEVN 60
           M    V FLALLLLASP+ +VA CQSD++  +S   +VV E +D  +  EED  D  ++N
Sbjct: 1   MMNLRVLFLALLLLASPLLQVARCQSDAEDHSSLVDDVVGENTDDAV--EEDDHDL-DMN 60

Query: 61  FAPAPGVDTVCVFPKNSARIVPAGEETELLAGVKNDGESSLNIIAIKASVHYTFDHHMLI 120
            +  PGV+TVCVFPKNSA++VPAGEETELL G+KN+G++ + ++ I+ASVH  +DH +L+
Sbjct: 61  LSSFPGVETVCVFPKNSAKLVPAGEETELLVGLKNEGKTRVGVMGIRASVHLPYDHKLLV 120

Query: 121 QNLTGQVFHNATVHPSTQATFPYIFAVSKFLQPGNFDLVGTIFYEVNQNAYQSVFFNGTI 180
           QNLT    +NA++  S QATFPYIFAVS++LQPG FDLVG I Y+V    YQSVF+NGTI
Sbjct: 121 QNLTMLRLNNASIPTSLQATFPYIFAVSQYLQPGAFDLVGYIIYDVEGKPYQSVFYNGTI 180

Query: 181 EVVEASG 185
           EVVE+ G
Sbjct: 181 EVVESGG 184