Homology
BLAST of MC04g0357 vs. ExPASy Swiss-Prot
Match:
Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)
HSP 1 Score: 364.0 bits (933), Expect = 2.0e-99
Identity = 186/381 (48.82%), Postives = 258/381 (67.72%), Query Frame = 0
Query: 1 MSKYKLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIES 60
MS +L + ++++ K ++ A + +W Y+FGF ++A KCAI+LKI + IE+
Sbjct: 1 MSSDQLSKFLDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIEN 60
Query: 61 HGS--PMTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TGYAQTPLSR--LL 120
H S P+TL++LSSA++ SP L RI+RFL H+GIFKE T L TGY TPLSR ++
Sbjct: 61 HPSSQPVTLAELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMI 120
Query: 121 ASNSNNSMAPFLLLESNPVMLAPWHNLSALIKA----KDGTPFEATHGKDLWSYTAADPT 180
S+APF+L E+ P MLAPW LS+++ + PF+A HGKD+WS+ +P
Sbjct: 121 TRRDGKSLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPF 180
Query: 181 HNKIFNDAMACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGIN 240
+ + N+AMAC AR + VP + C +F+GV ++VDVGGG G ++ M+VK PWI+G N
Sbjct: 181 LSDMINEAMACDARRV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFN 240
Query: 241 FDLPHVVSVAQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPK 300
FDLPHV+ VA+ GV++VEG+MFD IP DA FI WVLHDW D+DCIKILKNC++A+P
Sbjct: 241 FDLPHVIEVAEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPP 300
Query: 301 KIGKVIIVEAVIDGNEENNLWD--------VGLLLDMVMMAHTNKGKERTSEEWAYVLHK 360
IGKV+IVE+VI N++ + D V L+LDMVMMAHT+ GKERT +EW +VL +
Sbjct: 301 NIGKVLIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKE 360
Query: 361 AGFTRYTITPVKAVQSVIQAF 365
AGF RY + + VQS+I A+
Sbjct: 361 AGFARYEVRDIDDVQSLIIAY 380
BLAST of MC04g0357 vs. ExPASy Swiss-Prot
Match:
Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)
HSP 1 Score: 287.0 bits (733), Expect = 3.1e-76
Identity = 159/354 (44.92%), Postives = 218/354 (61.58%), Query Frame = 0
Query: 24 EDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKLSSALNCSPP---- 83
E+ + ++W ++F F + +KCA+EL I D I SHG P+T+S++ +L + P
Sbjct: 5 EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64
Query: 84 ---LLYRILRFLTHRGIFKEETTAQ-NLTGYAQTPLSRLLASNSNNSMAPFLLLESNPVM 143
L RI+R L H+ +F E + N Y T S+ L +S +++P +L E+NP++
Sbjct: 65 NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124
Query: 144 LAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPAILEG 203
L PW L + K +PFE HG ++W ADP N N AM C+ I +LE
Sbjct: 125 LKPWQYLGKCAQEK-SSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE- 184
Query: 204 CGEIFEGV-GSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNM 263
+ F G+ GSLVDVGGG G+ ++ IVKA P IQGINFDLPHVV+ A E GV+HV G+M
Sbjct: 185 YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDM 244
Query: 264 FDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAI-PKKIGKVIIVEAVI--DGNEENNL 323
F IP+ADA + W+LHDW+DEDC ILKNC AI KK GKVIIV+ V+ DGN+ L
Sbjct: 245 FVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGND---L 304
Query: 324 WD-VGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 365
+D +GL+ D++MMAHT GKERT EW +L+ AGF RY + A +I+AF
Sbjct: 305 FDKMGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAF 353
BLAST of MC04g0357 vs. ExPASy Swiss-Prot
Match:
B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)
HSP 1 Score: 279.6 bits (714), Expect = 4.9e-74
Identity = 161/368 (43.75%), Postives = 227/368 (61.68%), Query Frame = 0
Query: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
M+LA + T E +W I G + ++KCA+EL+I D + SH +P+TL+++
Sbjct: 1 MELARNDQT----EAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQI 60
Query: 71 SSALNCSPPL----LYRILRFLTHRGIFKEETTAQNLTG----YAQTPLSRLLASNS--- 130
+S++ SP L L RI+R L R IF + ++L G Y T SRLL S +
Sbjct: 61 ASSVPDSPSLNLSYLSRIMRLLVRRKIFSQH---KSLDGEEVLYGPTHSSRLLLSKTTLP 120
Query: 131 -NNSMAPFLLLESNPVMLAPWHNLSALIKAKDGTPFEATH-GKDLWSYTAADPTHNKIFN 190
++APF+ ++P + APW L+ +K + G FE H G+ LW + +P NK+FN
Sbjct: 121 DQVTLAPFVAFMTHPYLSAPWSCLARCVK-EGGNGFEMVHGGRQLWDLSPGNPEFNKVFN 180
Query: 191 DAMACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHV 250
D MA AR+ T+ AIL ++F G+ SLVDVGG G S+S IVK+ P I+GIN+DLPHV
Sbjct: 181 DGMASTARITTM-AILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHV 240
Query: 251 VSVAQECVG-VQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKV 310
V+ A G V HV GNMF+ IP A A F+ W+LHDW DEDC+KILKNCR A+P+K GK+
Sbjct: 241 VATAPTYTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKI 300
Query: 311 IIVEAVIDGNEENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKA 365
IIV+ V++ D ++LD+ +MA T +GKERT +EW VL + GF RY I + A
Sbjct: 301 IIVDIVLEPEGNGLFDDAAVMLDIALMALT-RGKERTEKEWKRVLEEGGFPRYQILKIPA 358
BLAST of MC04g0357 vs. ExPASy Swiss-Prot
Match:
Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)
HSP 1 Score: 271.9 bits (694), Expect = 1.0e-71
Identity = 141/345 (40.87%), Postives = 218/345 (63.19%), Query Frame = 0
Query: 23 EEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKLSSALNCSP---P 82
++D+ ++ ++WN+I+GF E ++KCA++L +A+ I + G+ MTLS+LSS L P
Sbjct: 4 KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNED 63
Query: 83 LLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLESNPVMLAPW 142
LYR++R+L H +F + + L Y P ++ L + M +L ++ +APW
Sbjct: 64 ALYRVMRYLVHMKLFTKASIDGELR-YGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPW 123
Query: 143 HNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPAILEGCGEI 202
H L + + GT FE G ++W Y A P N++FN+AMA +R+I + A+++ CG I
Sbjct: 124 HYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLI-MSALVKECGNI 183
Query: 203 FEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNMFDCIP 262
F G+ +LVDVGGG GT++ I A P I+ +DLPHV++ + V V G+MF IP
Sbjct: 184 FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 243
Query: 263 KADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENNLWDVGLLLD 322
KADA + +LHDW+D++CI+ILK C++A+P K GKVIIV+ V++ E+ + L LD
Sbjct: 244 KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 303
Query: 323 MVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 365
+ MM +T GKERT EEW ++H AG+ + IT + AVQSVI+A+
Sbjct: 304 LDMMLNTG-GKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345
BLAST of MC04g0357 vs. ExPASy Swiss-Prot
Match:
Q6WUC1 ((RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT PE=1 SV=1)
HSP 1 Score: 267.3 bits (682), Expect = 2.5e-70
Identity = 144/353 (40.79%), Postives = 221/353 (62.61%), Query Frame = 0
Query: 17 ESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKLSSALNC 76
E+ SK ++ ++ ++W I+GF E ++KCA++L+IA+T+ ++ PM+LS+L+S L
Sbjct: 2 ETVSKIDQQNQ---AKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPV 61
Query: 77 SPPL----LYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLESN 136
+ P+ L+RI+R+L H +FK + T Q Y+ P ++ L SM +L ++
Sbjct: 62 AQPVNEDRLFRIMRYLVHMELFKIDATTQK---YSLAPPAKYLLRGWEKSMVDSILCIND 121
Query: 137 PVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPAI 196
LAPWH+L + + FE GK +W Y + +P N++FN AMAC R++T A+
Sbjct: 122 KDFLAPWHHLGDGLTG-NCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVT-SAL 181
Query: 197 LEGCGEIF-EGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVE 256
C IF +G+ +LVDVGGG GT++ I KA P I+ +DLPHV++ + E + +
Sbjct: 182 ANECKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKIS 241
Query: 257 GNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENNL 316
G+MF IP ADA F+ +LHDWND++CI+ILK C++A+PK GKVIIV+ VID + +
Sbjct: 242 GDMFKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPKG-GKVIIVDVVIDMDSTHPY 301
Query: 317 WDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 365
+ L LD+ MM +T GKERT EEW + AGF + +T + AVQSVI+A+
Sbjct: 302 AKIRLTLDLDMMLNTG-GKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAY 344
BLAST of MC04g0357 vs. NCBI nr
Match:
XP_022135713.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])
HSP 1 Score: 742 bits (1916), Expect = 1.92e-270
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0
Query: 1 MSKYKLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIES 60
MSKYKLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIES
Sbjct: 1 MSKYKLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIES 60
Query: 61 HGSPMTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSN 120
HGSPMTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSN
Sbjct: 61 HGSPMTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSN 120
Query: 121 NSMAPFLLLESNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAM 180
NSMAPFLLLESNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAM
Sbjct: 121 NSMAPFLLLESNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAM 180
Query: 181 ACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSV 240
ACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSV
Sbjct: 181 ACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSV 240
Query: 241 AQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVE 300
AQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVE
Sbjct: 241 AQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVE 300
Query: 301 AVIDGNEENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSV 360
AVIDGNEENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSV
Sbjct: 301 AVIDGNEENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSV 360
Query: 361 IQAFI 365
IQAFI
Sbjct: 361 IQAFI 365
BLAST of MC04g0357 vs. NCBI nr
Match:
XP_022136086.1 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica charantia])
HSP 1 Score: 666 bits (1719), Expect = 1.41e-240
Identity = 322/355 (90.70%), Postives = 341/355 (96.06%), Query Frame = 0
Query: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
MDLALKESTSKKEEDHE A VQMWNYIFGFT++AIIKCAIELKIADTIESHGSPMTLS+L
Sbjct: 1 MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
Query: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASN NNSMAP LLLE
Sbjct: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
Query: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
S+P+MLA WHNLSA IKA DGTPFEATHGKDLWSYTAADPTHN+IFNDAMACAARVITVP
Sbjct: 121 SSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITVP 180
Query: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWI+GINFDLPHVVSVAQEC+GVQHV
Sbjct: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQHV 240
Query: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
EGNMFDCIPKADAAF+M VLHDWNDEDCIKILKNC+DA+PKK GKVIIVE VIDGNEE N
Sbjct: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEKN 300
Query: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365
L DVGL+LDMVMMA TNKGKERT EEWA+VLH++GF+RYTITP++A+QSVIQAFI
Sbjct: 301 LSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355
BLAST of MC04g0357 vs. NCBI nr
Match:
XP_023554119.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 575 bits (1483), Expect = 1.21e-204
Identity = 275/354 (77.68%), Postives = 312/354 (88.14%), Query Frame = 0
Query: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
MD ALK+S SKKEE HEHAR+Q+W YIFGF E+AIIKCAIELKI DTI+SHG MTL +L
Sbjct: 1 MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60
Query: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
SS+LNCS LL+RI+RFL HRGIFK+E T +NLT Y+ TPLSRLL S S NSMAPF+LLE
Sbjct: 61 SSSLNCSSSLLHRIMRFLVHRGIFKQEITGENLTRYSHTPLSRLLTSGSENSMAPFVLLE 120
Query: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
SNPVMLAPWH+LSA IK + PFEA HGKD+WSY AADP HN +FNDAMAC+ARV++VP
Sbjct: 121 SNPVMLAPWHSLSARIKGNEAIPFEAAHGKDIWSYAAADPIHNAMFNDAMACSARVMSVP 180
Query: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
AILE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+GINFDLPHV++ +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVIASLPADIGVQHV 240
Query: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
GNMFDCIPKADAAF+MWVLHDW+DE CIKILKNCRDAIPKK GKVIIVEAV++G +ENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQENN 300
Query: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 364
L DVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITP++A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 353
BLAST of MC04g0357 vs. NCBI nr
Match:
XP_038888665.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])
HSP 1 Score: 573 bits (1478), Expect = 7.25e-204
Identity = 275/355 (77.46%), Postives = 309/355 (87.04%), Query Frame = 0
Query: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
MD A KES KKEE+ E ARVQ+WNYIFGF E+AI+KCAIELKIADTIE+HGS MTLS+L
Sbjct: 1 MDFAFKESIPKKEEEDEQARVQIWNYIFGFVEIAIVKCAIELKIADTIETHGSSMTLSQL 60
Query: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
S ALNCS LLYRILRFL +RGIFK+E T +NLT Y+QTPLSRLLAS++NNSM P +LLE
Sbjct: 61 SLALNCSSSLLYRILRFLVNRGIFKQEITEENLTSYSQTPLSRLLASSNNNSMVPLVLLE 120
Query: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
S+PVMLAPWH LS IKA +GTPFEA HGKDLWS+ AADP HN I N+AM+C ARV+TVP
Sbjct: 121 SSPVMLAPWHRLSDRIKANEGTPFEAAHGKDLWSFAAADPIHNTIINEAMSCTARVLTVP 180
Query: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
AILE C EIFEG+GSLVDVGGGNGT LSMIVKACPWI+GIN DLPHV+S +QE GVQHV
Sbjct: 181 AILEDCPEIFEGIGSLVDVGGGNGTCLSMIVKACPWIKGINLDLPHVISSSQEYTGVQHV 240
Query: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
G+M D IPKADAAF MWVLHDW+DE CIKILKNCRDAIP+K GKVIIVEAVI+ EENN
Sbjct: 241 SGSMLDSIPKADAAFFMWVLHDWDDETCIKILKNCRDAIPEKTGKVIIVEAVIEEKEENN 300
Query: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365
L DVGL+LDMVMMAHTN GKERT++EW YVLH+AGFTRYTITP++AVQSVIQ F+
Sbjct: 301 LSDVGLMLDMVMMAHTNNGKERTAKEWGYVLHQAGFTRYTITPIRAVQSVIQGFL 355
BLAST of MC04g0357 vs. NCBI nr
Match:
XP_022952490.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita moschata])
HSP 1 Score: 572 bits (1473), Expect = 4.03e-203
Identity = 277/354 (78.25%), Postives = 312/354 (88.14%), Query Frame = 0
Query: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
MD ALK+S SKKEE HEHAR+Q+W YIFGF E+AIIKCAIELKI DTI+SHG MTL +L
Sbjct: 1 MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60
Query: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
SS+LNCS LLYRI+RFL HRGIFKEE T +NLT Y+ TPLSRLLAS+S++SMAP LLLE
Sbjct: 61 SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120
Query: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
SNPVMLAPWH LSA IK + PFEA HGKD+WSY AA+PTHN +FNDAMAC+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180
Query: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
AILE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+GINFDLPHVV+ + +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHV 240
Query: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
GNMFDCIPKADAAF+MWVLH W+DE CIKILK CR+AIPKK GKVIIVEAV++ EENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENN 300
Query: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 364
L DVGL+LDMVMMAHTN GKERT+EEWAYVLH+AGFTR+TITP++A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353
BLAST of MC04g0357 vs. ExPASy TrEMBL
Match:
A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)
HSP 1 Score: 742 bits (1916), Expect = 9.32e-271
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0
Query: 1 MSKYKLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIES 60
MSKYKLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIES
Sbjct: 1 MSKYKLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIES 60
Query: 61 HGSPMTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSN 120
HGSPMTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSN
Sbjct: 61 HGSPMTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSN 120
Query: 121 NSMAPFLLLESNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAM 180
NSMAPFLLLESNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAM
Sbjct: 121 NSMAPFLLLESNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAM 180
Query: 181 ACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSV 240
ACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSV
Sbjct: 181 ACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSV 240
Query: 241 AQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVE 300
AQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVE
Sbjct: 241 AQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVE 300
Query: 301 AVIDGNEENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSV 360
AVIDGNEENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSV
Sbjct: 301 AVIDGNEENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSV 360
Query: 361 IQAFI 365
IQAFI
Sbjct: 361 IQAFI 365
BLAST of MC04g0357 vs. ExPASy TrEMBL
Match:
A0A6J1C2I8 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111007866 PE=3 SV=1)
HSP 1 Score: 666 bits (1719), Expect = 6.81e-241
Identity = 322/355 (90.70%), Postives = 341/355 (96.06%), Query Frame = 0
Query: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
MDLALKESTSKKEEDHE A VQMWNYIFGFT++AIIKCAIELKIADTIESHGSPMTLS+L
Sbjct: 1 MDLALKESTSKKEEDHEQAHVQMWNYIFGFTKMAIIKCAIELKIADTIESHGSPMTLSQL 60
Query: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASN NNSMAP LLLE
Sbjct: 61 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNGNNSMAPLLLLE 120
Query: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
S+P+MLA WHNLSA IKA DGTPFEATHGKDLWSYTAADPTHN+IFNDAMACAARVITVP
Sbjct: 121 SSPIMLASWHNLSARIKASDGTPFEATHGKDLWSYTAADPTHNRIFNDAMACAARVITVP 180
Query: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWI+GINFDLPHVVSVAQEC+GVQHV
Sbjct: 181 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIRGINFDLPHVVSVAQECMGVQHV 240
Query: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
EGNMFDCIPKADAAF+M VLHDWNDEDCIKILKNC+DA+PKK GKVIIVE VIDGNEE N
Sbjct: 241 EGNMFDCIPKADAAFLMSVLHDWNDEDCIKILKNCKDALPKKTGKVIIVETVIDGNEEKN 300
Query: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAFI 365
L DVGL+LDMVMMA TNKGKERT EEWA+VLH++GF+RYTITP++A+QSVIQAFI
Sbjct: 301 LSDVGLMLDMVMMAQTNKGKERTCEEWAFVLHESGFSRYTITPIRAIQSVIQAFI 355
BLAST of MC04g0357 vs. ExPASy TrEMBL
Match:
A0A6J1GLX2 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455163 PE=3 SV=1)
HSP 1 Score: 572 bits (1473), Expect = 1.95e-203
Identity = 277/354 (78.25%), Postives = 312/354 (88.14%), Query Frame = 0
Query: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
MD ALK+S SKKEE HEHAR+Q+W YIFGF E+AIIKCAIELKI DTI+SHG MTL +L
Sbjct: 1 MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60
Query: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
SS+LNCS LLYRI+RFL HRGIFKEE T +NLT Y+ TPLSRLLAS+S++SMAP LLLE
Sbjct: 61 SSSLNCSSSLLYRIMRFLVHRGIFKEEITDENLTCYSHTPLSRLLASSSDSSMAPLLLLE 120
Query: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
SNPVMLAPWH LSA IK + PFEA HGKD+WSY AA+PTHN +FNDAMAC+ARV+TVP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMTVP 180
Query: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
AILE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+GINFDLPHVV+ + +GVQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKGINFDLPHVVASSPPYIGVQHV 240
Query: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
GNMFDCIPKADAAF+MWVLH W+DE CIKILK CR+AIPKK GKVIIVEAV++ EENN
Sbjct: 241 GGNMFDCIPKADAAFLMWVLHLWDDETCIKILKKCREAIPKKTGKVIIVEAVLEEKEENN 300
Query: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 364
L DVGL+LDMVMMAHTN GKERT+EEWAYVLH+AGFTR+TITP++A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAEEWAYVLHQAGFTRHTITPIRALQSVIQAF 353
BLAST of MC04g0357 vs. ExPASy TrEMBL
Match:
A0A6J1GLX9 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455166 PE=3 SV=1)
HSP 1 Score: 556 bits (1433), Expect = 2.08e-197
Identity = 271/354 (76.55%), Postives = 306/354 (86.44%), Query Frame = 0
Query: 11 MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70
MD ALK+S SKKEE HEHAR+Q+W YIFGF E+AIIKCAIELKI DTI+SHG MTL +L
Sbjct: 1 MDFALKQSISKKEE-HEHARLQVWQYIFGFVEMAIIKCAIELKIGDTIDSHGGLMTLPEL 60
Query: 71 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 130
SS+LNCS LLYRI+RFL HRGIFKEE T GYAQTP+SRLLAS+S +SMAP LL E
Sbjct: 61 SSSLNCSSSLLYRIMRFLVHRGIFKEEPTK----GYAQTPMSRLLASSSGSSMAPLLLFE 120
Query: 131 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 190
SNPVMLAPWH LSA IK + PFEA HGKD+WSY AA+PTHN +FNDAMAC+ARV++VP
Sbjct: 121 SNPVMLAPWHGLSARIKGNEAIPFEAAHGKDMWSYAAANPTHNAMFNDAMACSARVMSVP 180
Query: 191 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 250
AILE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+ INFDLPHV++ + VQHV
Sbjct: 181 AILEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVIASLPADIDVQHV 240
Query: 251 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 310
GNMFD IPKADAAF+MWVLHDW+DE CIKILKNCR+AIPKK GKVIIVEAV++ EENN
Sbjct: 241 GGNMFDSIPKADAAFLMWVLHDWDDETCIKILKNCREAIPKKTGKVIIVEAVLEEKEENN 300
Query: 311 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 364
L DVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITP++A+QSVIQAF
Sbjct: 301 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRALQSVIQAF 349
BLAST of MC04g0357 vs. ExPASy TrEMBL
Match:
A0A6J1GLV5 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455165 PE=3 SV=1)
HSP 1 Score: 547 bits (1410), Expect = 7.34e-194
Identity = 265/354 (74.86%), Postives = 304/354 (85.88%), Query Frame = 0
Query: 12 DLALKESTSK-KEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 71
D+ K+S K +EE++ +VQ+W YIFGF E+A+IKCAIELKI DTIE+HG MTL+ L
Sbjct: 3 DIGSKDSKLKIEEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTLTDL 62
Query: 72 SSALNCSPPLLYRILRFLTHRGIFKEETTAQNLTGYAQTPLSRLLASNSNNSMAPFLLLE 131
SS+LNCSP LL+RILRFL HRGIFKEE T YAQTP+SRLLAS+S +SMAP LL E
Sbjct: 63 SSSLNCSPLLLHRILRFLVHRGIFKEEPTKS----YAQTPMSRLLASSSGSSMAPLLLFE 122
Query: 132 SNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVP 191
SNPVMLAPWH+LSA IK + PFEA HGKDLWSY AADP HN +FNDAMAC+ARV++VP
Sbjct: 123 SNPVMLAPWHSLSARIKGNEAIPFEAAHGKDLWSYAAADPIHNTMFNDAMACSARVMSVP 182
Query: 192 AILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHV 251
+LE CGEIFEGV LVDVGGGNGTSLSMIVKACPWI+ INFDLPHVV+ + +GVQHV
Sbjct: 183 TLLEDCGEIFEGVECLVDVGGGNGTSLSMIVKACPWIKAINFDLPHVVASSPPPIGVQHV 242
Query: 252 EGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENN 311
GNMFDCIPKADAAF+MWVLHDW+DE CIKILKNCRDAIPKK GKVIIVEAV++G +ENN
Sbjct: 243 GGNMFDCIPKADAAFLMWVLHDWDDETCIKILKNCRDAIPKKTGKVIIVEAVLEGKQENN 302
Query: 312 LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 364
L DVGL+LDMVMMAHTN GKERT++EWAYVLH+AGFTR+TITP++AVQSVIQAF
Sbjct: 303 LSDVGLMLDMVMMAHTNDGKERTAKEWAYVLHQAGFTRHTITPIRAVQSVIQAF 352
BLAST of MC04g0357 vs. TAIR 10
Match:
AT4G35160.1 (O-methyltransferase family protein )
HSP 1 Score: 364.0 bits (933), Expect = 1.4e-100
Identity = 186/381 (48.82%), Postives = 258/381 (67.72%), Query Frame = 0
Query: 1 MSKYKLIALIMDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIES 60
MS +L + ++++ K ++ A + +W Y+FGF ++A KCAI+LKI + IE+
Sbjct: 1 MSSDQLSKFLDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIEN 60
Query: 61 HGS--PMTLSKLSSALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TGYAQTPLSR--LL 120
H S P+TL++LSSA++ SP L RI+RFL H+GIFKE T L TGY TPLSR ++
Sbjct: 61 HPSSQPVTLAELSSAVSASPSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMI 120
Query: 121 ASNSNNSMAPFLLLESNPVMLAPWHNLSALIKA----KDGTPFEATHGKDLWSYTAADPT 180
S+APF+L E+ P MLAPW LS+++ + PF+A HGKD+WS+ +P
Sbjct: 121 TRRDGKSLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPF 180
Query: 181 HNKIFNDAMACAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGIN 240
+ + N+AMAC AR + VP + C +F+GV ++VDVGGG G ++ M+VK PWI+G N
Sbjct: 181 LSDMINEAMACDARRV-VPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFN 240
Query: 241 FDLPHVVSVAQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPK 300
FDLPHV+ VA+ GV++VEG+MFD IP DA FI WVLHDW D+DCIKILKNC++A+P
Sbjct: 241 FDLPHVIEVAEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPP 300
Query: 301 KIGKVIIVEAVIDGNEENNLWD--------VGLLLDMVMMAHTNKGKERTSEEWAYVLHK 360
IGKV+IVE+VI N++ + D V L+LDMVMMAHT+ GKERT +EW +VL +
Sbjct: 301 NIGKVLIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKE 360
Query: 361 AGFTRYTITPVKAVQSVIQAF 365
AGF RY + + VQS+I A+
Sbjct: 361 AGFARYEVRDIDDVQSLIIAY 380
BLAST of MC04g0357 vs. TAIR 10
Match:
AT4G35150.1 (O-methyltransferase family protein )
HSP 1 Score: 307.4 bits (786), Expect = 1.6e-83
Identity = 163/361 (45.15%), Postives = 221/361 (61.22%), Query Frame = 0
Query: 15 LKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGS--PMTLSKLSS 74
++ES ++ A + +W Y+FGF ++A KCAI+LKI + IE+H S P+TLS+LSS
Sbjct: 1 MEESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSS 60
Query: 75 ALNCSPPLLYRILRFLTHRGIFKEETTAQNL-TGYAQTPLSRLLASNSNNSMAPFLLLES 134
A++ SP L RI+RFL H+G+FKE T L TGY TPLSR +
Sbjct: 61 AVSASPSHLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMM--------------- 120
Query: 135 NPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPA 194
HGKDLW++ + H+++ N+AMAC AR + VP
Sbjct: 121 ----------------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPR 180
Query: 195 ILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVE 254
+ C +F+GV ++VDVGGG G ++ ++VK PWI+G NFDLPHV+ VAQ GV++VE
Sbjct: 181 VAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVE 240
Query: 255 GNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNE---- 314
G+MFD IP +DA I WVLHDW D+DCIKILKNC++A+ IGKV+IVE VI +
Sbjct: 241 GDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMI 300
Query: 315 ----ENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQSVIQA 365
++ L V L LDMVMM HT+ GKERT +EW +VL +AGF RY + VQS+I A
Sbjct: 301 AEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIA 323
BLAST of MC04g0357 vs. TAIR 10
Match:
AT5G54160.1 (O-methyltransferase 1 )
HSP 1 Score: 201.8 bits (512), Expect = 9.2e-52
Identity = 121/331 (36.56%), Postives = 190/331 (57.40%), Query Frame = 0
Query: 46 IKCAIELKIADTIESHGSPMTLSKLSSALNC----SPPLLYRILRFLTHRGIFKEETTAQ 105
+K A+EL + + + +GSPM+ ++++S L +P +L RILR LT + + +
Sbjct: 36 LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVL--TCSNR 95
Query: 106 NLTG------YAQTPLSRLLASNSNN-SMAPFLLLESNPVMLAPWHNLSALIKAKDGTPF 165
L+G Y P+ + L N + S+A L+ + V++ W++L I G PF
Sbjct: 96 KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIPF 155
Query: 166 EATHGKDLWSYTAADPTHNKIFNDAMACAARVITVPAILEGCGEIFEGVGSLVDVGGGNG 225
+G + Y DP NK+FN+ M+ + IT+ ILE + FEG+ SLVDVGGG G
Sbjct: 156 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHS-TITMKKILE-TYKGFEGLTSLVDVGGGIG 215
Query: 226 TSLSMIVKACPWIQGINFDLPHVVSVAQECVGVQHVEGNMFDCIPKADAAFIMWVLHDWN 285
+L MIV P ++GINFDLPHV+ A G++HV G+MF +PK DA F+ W+ HDW+
Sbjct: 216 ATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWS 275
Query: 286 DEDCIKILKNCRDAIPKKIGKVIIVEAVIDGNEENNLWDVGLL-LDMVMMAHTNKGKERT 345
DE C+K LKNC +++P+ GKVI+ E ++ +++L ++ +D +M+AH GKERT
Sbjct: 276 DEHCVKFLKNCYESLPED-GKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERT 335
Query: 346 SEEWAYVLHKAGFTRYTITPVKAVQSVIQAF 365
+E+ + +GF K ++ V AF
Sbjct: 336 EKEFEALAKASGF--------KGIKVVCDAF 352
BLAST of MC04g0357 vs. TAIR 10
Match:
AT1G77530.1 (O-methyltransferase family protein )
HSP 1 Score: 186.8 bits (473), Expect = 3.1e-47
Identity = 127/363 (34.99%), Postives = 201/363 (55.37%), Query Frame = 0
Query: 21 KKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTI--ESHGSPMTLSKLSSALNC-- 80
++E D + +Q + + ++K A+EL + DTI S+G+ ++ S+++ +L
Sbjct: 21 EQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKP 80
Query: 81 ----SPPLLYRILRFLTHRGIFK---EETTAQNLTG-----YAQTPLSR--LLASNSNNS 140
+P LL R+LR L I K E+ TG YA P+ + L S+ + S
Sbjct: 81 TNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGS 140
Query: 141 MAPFLLLESNPVMLAPWHNL-SALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMA 200
++ LLL + V+L W NL +++ KD F + H L+ Y ++D +K+F+ AM+
Sbjct: 141 LSSLLLLLHSQVILKTWTNLKDVILEGKDA--FSSAHDMRLFEYISSDDQFSKLFHRAMS 200
Query: 201 CAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVA 260
++ ++ + E G FE V +LVDVGGG GT L +I P I+G+NFDL V++ A
Sbjct: 201 ESSTMVMKKVLEEYRG--FEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQA 260
Query: 261 QECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEA 320
GV+HV G+MF +PK DA F+ W+LHDW DEDCIKILKNC ++P+K GKVIIVE
Sbjct: 261 PFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEK-GKVIIVEM 320
Query: 321 VIDGNEENNLWDVGLLL--DMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQS 363
+ + N + +L D++M+ + GKER+ ++ + +GF I + S
Sbjct: 321 ITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYS 378
BLAST of MC04g0357 vs. TAIR 10
Match:
AT1G77520.1 (O-methyltransferase family protein )
HSP 1 Score: 181.8 bits (460), Expect = 9.9e-46
Identity = 123/363 (33.88%), Postives = 186/363 (51.24%), Query Frame = 0
Query: 21 KKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKLSSALNC---- 80
++E D + +Q + + ++K A EL + DTI + G+ LS A +
Sbjct: 21 EQEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKP 80
Query: 81 ----SPPLLYRILRFLTHRGIFK---EETTAQNLTG-----YAQTPLSR--LLASNSNNS 140
+P LL R+L L I K ET TG YA P+ + L S+ S
Sbjct: 81 TNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGS 140
Query: 141 MAPFLLLESNPVMLAPWHNL-SALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMA 200
+ P +L V W NL +++ +D F + HG ++ Y +D ++FN AM+
Sbjct: 141 LVPLFMLLHTQVFFKTWTNLKDVILEGRDA--FNSAHGMKIFEYINSDQPFAELFNRAMS 200
Query: 201 CAARVITVPAILEGCGEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQGINFDLPHVVSVA 260
+ +I + G FE V +LVDVGGGNGT L ++ P I+G+NFDL V++ A
Sbjct: 201 EPSTMIMKKVLDVYRG--FEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQA 260
Query: 261 QECVGVQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEA 320
GV+HV G+MF +PK DA F+ W+LHDW DEDCIKILKNC ++P+K GK+IIVE
Sbjct: 261 PFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEF 320
Query: 321 VIDGNEENN--LWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVKAVQS 363
V + + +D++M+ + GKER+ ++ + +GF R I + S
Sbjct: 321 VTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYS 378
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9T003 | 2.0e-99 | 48.82 | Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1... | [more] |
Q6WUC2 | 3.1e-76 | 44.92 | (R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=... | [more] |
B0ZB56 | 4.9e-74 | 43.75 | Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1 | [more] |
Q9LEL6 | 1.0e-71 | 40.87 | (RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1 | [more] |
Q6WUC1 | 2.5e-70 | 40.79 | (RS)-norcoclaurine 6-O-methyltransferase OS=Papaver somniferum OX=3469 GN=6OMT P... | [more] |
Match Name | E-value | Identity | Description | |
XP_022135713.1 | 1.92e-270 | 100.00 | (R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia] | [more] |
XP_022136086.1 | 1.41e-240 | 90.70 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like [Momordica char... | [more] |
XP_023554119.1 | 1.21e-204 | 77.68 | (RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita pepo subsp. pepo] | [more] |
XP_038888665.1 | 7.25e-204 | 77.46 | acetylserotonin O-methyltransferase-like [Benincasa hispida] | [more] |
XP_022952490.1 | 4.03e-203 | 78.25 | (RS)-norcoclaurine 6-O-methyltransferase-like [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1C5N1 | 9.32e-271 | 100.00 | (R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO... | [more] |
A0A6J1C2I8 | 6.81e-241 | 90.70 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Momordica ch... | [more] |
A0A6J1GLX2 | 1.95e-203 | 78.25 | (RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L... | [more] |
A0A6J1GLX9 | 2.08e-197 | 76.55 | 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2-like OS=Cucurbita mo... | [more] |
A0A6J1GLV5 | 7.34e-194 | 74.86 | (RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L... | [more] |