MC04g0329 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0329
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	F-box domain-containing protein
Location	MC04: 2595486 .. 2595866 (+)
RNA-Seq Expression	MC04g0329
Synteny	MC04g0329

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGAAGATCTGCCAAAGGATGTTATGGAGAAAATTGGTTTGAATCTTAATTCAACTGATTACATTCATGCCTCTGTTGTTTGCAAATCATGGCTATCACTTTTACAATCTGATATTTCAAGACCTCAAATTCCTTGGATTTTGCTTCCTCATCAATCTGATGACCATAGCCTAAAATTCTTCAACTTTTTGGAAGTCAAATATCACATCTTAAGGTTGCCTAAGGGTCTTGAAGGGGGATATATTTTTGGGAGTTCCAAAGGATGGCTTGTTGTTGTGTTTAGGAGAAGAAGAAGATCAGGATGGTACCATGTCTATCTTTTGAATCCAATCTCAGGTGTCAAGTGTAGGCTTCCGCCGCTTCCAAATTATATATCTCTT

mRNA sequence

TGGGAAGATCTGCCAAAGGATGTTATGGAGAAAATTGGTTTGAATCTTAATTCAACTGATTACATTCATGCCTCTGTTGTTTGCAAATCATGGCTATCACTTTTACAATCTGATATTTCAAGACCTCAAATTCCTTGGATTTTGCTTCCTCATCAATCTGATGACCATAGCCTAAAATTCTTCAACTTTTTGGAAGTCAAATATCACATCTTAAGGTTGCCTAAGGGTCTTGAAGGGGGATATATTTTTGGGAGTTCCAAAGGATGGCTTGTTGTTGTGTTTAGGAGAAGAAGAAGATCAGGATGGTACCATGTCTATCTTTTGAATCCAATCTCAGGTGTCAAGTGTAGGCTTCCGCCGCTTCCAAATTATATATCTCTT

Coding sequence (CDS)

TGGGAAGATCTGCCAAAGGATGTTATGGAGAAAATTGGTTTGAATCTTAATTCAACTGATTACATTCATGCCTCTGTTGTTTGCAAATCATGGCTATCACTTTTACAATCTGATATTTCAAGACCTCAAATTCCTTGGATTTTGCTTCCTCATCAATCTGATGACCATAGCCTAAAATTCTTCAACTTTTTGGAAGTCAAATATCACATCTTAAGGTTGCCTAAGGGTCTTGAAGGGGGATATATTTTTGGGAGTTCCAAAGGATGGCTTGTTGTTGTGTTTAGGAGAAGAAGAAGATCAGGATGGTACCATGTCTATCTTTTGAATCCAATCTCAGGTGTCAAGTGTAGGCTTCCGCCGCTTCCAAATTATATATCTCTT

Protein sequence

WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPHQSDDHSLKFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRLPPLPNYISL

Homology

[Close]

BLAST of MC04g0329 vs. ExPASy Swiss-Prot
Match: Q9FVS1 (F-box/kelch-repeat protein At1g57790 OS=Arabidopsis thaliana OX=3702 GN=At1g57790 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 8.1e-08
Identity = 35/119 (29.41%), Postives = 54/119 (45.38%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPHQSDDHSLKF 60
           W+DLP +++  +   L   D + ASVVCKSW     S     + PW L+      ++  F
Sbjct: 13  WKDLPLELLSSVMTFLEIKDNVRASVVCKSWFEAAVSVRVIDKSPW-LMYFPETKNTYDF 72

Query: 61  FNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRLP 120
           ++    K + + LPK L G  +  S  GWL++      +    H  L NP +     LP
Sbjct: 73  YDPSNCKKYTMELPKSLVGFIVRYSKDGWLLM-----SQEDSSHFVLFNPFTMDVVALP 125

[Close]

BLAST of MC04g0329 vs. ExPASy Swiss-Prot
Match: Q9LSA5 (F-box/kelch-repeat protein At3g18720 OS=Arabidopsis thaliana OX=3702 GN=At3g18720 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.5e-06
Identity = 38/126 (30.16%), Postives = 59/126 (46.83%), Query Frame = 0


Query: 3   DLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPHQS----DDHSL 62
           DL ++++ ++GL  N    IHAS+VCK+WL    S       PW+  P        +   
Sbjct: 55  DLLQEILSRLGLKAN----IHASLVCKTWLKEAVSVRKFQSRPWLFYPQSQRGGPKEGDY 114

Query: 63  KFFNFLEVKYHILRLPKGLEG--GYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKC 122
             FN    + H L+ P+ L G    +  +  GWL+VV     +     V+ LNP +G + 
Sbjct: 115 VLFNPSRSQTHHLKFPE-LTGYRNKLACAKDGWLLVV-----KDNPDVVFFLNPFTGERI 170

[Close]

BLAST of MC04g0329 vs. NCBI nr
Match: XP_022155350.1 (uncharacterized protein LOC111022492 [Momordica charantia])

HSP 1 Score: 125 bits (313), Expect = 5.15e-31
Identity = 63/128 (49.22%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDISRPQIPWILLPHQSDDHSLK 60
           W++LP D+MEKI L LNSTDYI  S+VCKSW SLL QSDI RPQ PW+LLPH S  H++K
Sbjct: 28  WKNLPGDLMEKIALKLNSTDYIRNSMVCKSWQSLLLQSDILRPQFPWLLLPHHSSKHTIK 87

Query: 61  FFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRLP 120
            ++  E K+H   +     G  + G SKGW++       ++    + L NPISG KC L 
Sbjct: 88  LYSVSENKFHTFHVD---GGNNVVGCSKGWVIF------KTVGSKISLFNPISGAKCILS 146

Query: 121 PLPNYISL 127
            LP  +SL
Sbjct: 148 SLPTALSL 146

[Close]

BLAST of MC04g0329 vs. NCBI nr
Match: KAE8008804.1 (hypothetical protein FH972_005279 [Carpinus fangiana])

HSP 1 Score: 111 bits (277), Expect = 9.30e-26
Identity = 59/128 (46.09%), Postives = 78/128 (60.94%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPHQSDDHSL 60
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLPH  + +SL
Sbjct: 60  WDEVPYDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNCNSL 119

Query: 61  KFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 120
            FF+  E   H ++LPK  +GG+ FGSSKGWL +    R       V+LLNPISGV+  L
Sbjct: 120 SFFSMSEGIVHSIKLPKAAQGGWCFGSSKGWLTIA---RGTQDDPQVFLLNPISGVQRHL 179

Query: 121 PPLPNYIS 126
           P L    S
Sbjct: 180 PSLTTIPS 184

[Close]

BLAST of MC04g0329 vs. NCBI nr
Match: KAE8055702.1 (hypothetical protein FH972_012527 [Carpinus fangiana])

HSP 1 Score: 110 bits (276), Expect = 1.41e-25
Identity = 54/124 (43.55%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISR--PQIPWILLPHQSDDHSL 60
           W++LP  +ME I   LN  D +H+S VCKSW SL+     R  PQ+PW++LPH+   +SL
Sbjct: 40  WDELPDGIMENIFGRLNMIDLVHSSGVCKSWRSLVMQKQVRAAPQLPWLVLPHRQSCNSL 99

Query: 61  KFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWY-HVYLLNPISGVKCR 120
            F++  E   + ++LPK  +GG+ FG SK WL++     R + W   VYL NP SG K  
Sbjct: 100 SFYSMAEATVYNIKLPKPAQGGWCFGCSKSWLMMA----RGTEWEPEVYLFNPFSGFKLN 159

[Close]

BLAST of MC04g0329 vs. NCBI nr
Match: KAE8008846.1 (hypothetical protein FH972_005319 [Carpinus fangiana])

HSP 1 Score: 108 bits (269), Expect = 1.15e-24
Identity = 56/123 (45.53%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPHQSDDHSL 60
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLPH  + +SL
Sbjct: 52  WDEVPDDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNCNSL 111

Query: 61  KFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 120
            FF+  E   H ++LP+  +GG+ FGSSKGWL +    R       V+LL+PISGV+  L
Sbjct: 112 SFFSMSEGIVHTIKLPEAAQGGWCFGSSKGWLTIA---RGTQDDPQVFLLSPISGVQRHL 171

[Close]

BLAST of MC04g0329 vs. NCBI nr
Match: KAE8008803.1 (hypothetical protein FH972_005278 [Carpinus fangiana])

HSP 1 Score: 106 bits (264), Expect = 2.18e-24
Identity = 58/128 (45.31%), Postives = 75/128 (58.59%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPHQSDDHSL 60
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLPH    +SL
Sbjct: 63  WDEVPDDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAPICNSL 122

Query: 61  KFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 120
            FF+  E   H ++LPK   GG+ FGSSKGWL +    R       V+LLNPISG +  L
Sbjct: 123 SFFSMSEGIVHNIKLPKAAHGGWCFGSSKGWLTIA---RGTHDDPQVFLLNPISGFQRHL 182

Query: 121 PPLPNYIS 126
           P L    S
Sbjct: 183 PSLTTIPS 187

[Close]

BLAST of MC04g0329 vs. ExPASy TrEMBL
Match: A0A6J1DM79 (uncharacterized protein LOC111022492 OS=Momordica charantia OX=3673 GN=LOC111022492 PE=4 SV=1)

HSP 1 Score: 125 bits (313), Expect = 2.49e-31
Identity = 63/128 (49.22%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDISRPQIPWILLPHQSDDHSLK 60
           W++LP D+MEKI L LNSTDYI  S+VCKSW SLL QSDI RPQ PW+LLPH S  H++K
Sbjct: 28  WKNLPGDLMEKIALKLNSTDYIRNSMVCKSWQSLLLQSDILRPQFPWLLLPHHSSKHTIK 87

Query: 61  FFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRLP 120
            ++  E K+H   +     G  + G SKGW++       ++    + L NPISG KC L 
Sbjct: 88  LYSVSENKFHTFHVD---GGNNVVGCSKGWVIF------KTVGSKISLFNPISGAKCILS 146

Query: 121 PLPNYISL 127
            LP  +SL
Sbjct: 148 SLPTALSL 146

[Close]

BLAST of MC04g0329 vs. ExPASy TrEMBL
Match: A0A5N6QP56 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005279 PE=4 SV=1)

HSP 1 Score: 111 bits (277), Expect = 4.50e-26
Identity = 59/128 (46.09%), Postives = 78/128 (60.94%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPHQSDDHSL 60
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLPH  + +SL
Sbjct: 60  WDEVPYDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNCNSL 119

Query: 61  KFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 120
            FF+  E   H ++LPK  +GG+ FGSSKGWL +    R       V+LLNPISGV+  L
Sbjct: 120 SFFSMSEGIVHSIKLPKAAQGGWCFGSSKGWLTIA---RGTQDDPQVFLLNPISGVQRHL 179

Query: 121 PPLPNYIS 126
           P L    S
Sbjct: 180 PSLTTIPS 184

[Close]

BLAST of MC04g0329 vs. ExPASy TrEMBL
Match: A0A5N6R432 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_012527 PE=4 SV=1)

HSP 1 Score: 110 bits (276), Expect = 6.81e-26
Identity = 54/124 (43.55%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISR--PQIPWILLPHQSDDHSL 60
           W++LP  +ME I   LN  D +H+S VCKSW SL+     R  PQ+PW++LPH+   +SL
Sbjct: 40  WDELPDGIMENIFGRLNMIDLVHSSGVCKSWRSLVMQKQVRAAPQLPWLVLPHRQSCNSL 99

Query: 61  KFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWY-HVYLLNPISGVKCR 120
            F++  E   + ++LPK  +GG+ FG SK WL++     R + W   VYL NP SG K  
Sbjct: 100 SFYSMAEATVYNIKLPKPAQGGWCFGCSKSWLMMA----RGTEWEPEVYLFNPFSGFKLN 159

[Close]

BLAST of MC04g0329 vs. ExPASy TrEMBL
Match: A0A5N6QPU6 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005319 PE=4 SV=1)

HSP 1 Score: 108 bits (269), Expect = 5.54e-25
Identity = 56/123 (45.53%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPHQSDDHSL 60
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLPH  + +SL
Sbjct: 52  WDEVPDDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAQNCNSL 111

Query: 61  KFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 120
            FF+  E   H ++LP+  +GG+ FGSSKGWL +    R       V+LL+PISGV+  L
Sbjct: 112 SFFSMSEGIVHTIKLPEAAQGGWCFGSSKGWLTIA---RGTQDDPQVFLLSPISGVQRHL 171

[Close]

BLAST of MC04g0329 vs. ExPASy TrEMBL
Match: A0A5N6QRN4 (F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005278 PE=4 SV=1)

HSP 1 Score: 106 bits (264), Expect = 1.06e-24
Identity = 58/128 (45.31%), Postives = 75/128 (58.59%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLL-QSDI-SRPQIPWILLPHQSDDHSL 60
           W+++P D+M +I   LN  D I    VCKSW S++ Q DI S P +PW+LLPH    +SL
Sbjct: 63  WDEVPDDMMGEILGRLNIIDRIRLGGVCKSWRSIVKQKDIHSAPSVPWLLLPHAPICNSL 122

Query: 61  KFFNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRL 120
            FF+  E   H ++LPK   GG+ FGSSKGWL +    R       V+LLNPISG +  L
Sbjct: 123 SFFSMSEGIVHNIKLPKAAHGGWCFGSSKGWLTIA---RGTHDDPQVFLLNPISGFQRHL 182

Query: 121 PPLPNYIS 126
           P L    S
Sbjct: 183 PSLTTIPS 187

[Close]

BLAST of MC04g0329 vs. TAIR 10
Match: AT1G57790.1 (F-box family protein )

HSP 1 Score: 58.2 bits (139), Expect = 5.7e-09
Identity = 35/119 (29.41%), Postives = 54/119 (45.38%), Query Frame = 0


Query: 1   WEDLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPHQSDDHSLKF 60
           W+DLP +++  +   L   D + ASVVCKSW     S     + PW L+      ++  F
Sbjct: 13  WKDLPLELLSSVMTFLEIKDNVRASVVCKSWFEAAVSVRVIDKSPW-LMYFPETKNTYDF 72

Query: 61  FNFLEVKYHILRLPKGLEGGYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKCRLP 120
           ++    K + + LPK L G  +  S  GWL++      +    H  L NP +     LP
Sbjct: 73  YDPSNCKKYTMELPKSLVGFIVRYSKDGWLLM-----SQEDSSHFVLFNPFTMDVVALP 125

[Close]

BLAST of MC04g0329 vs. TAIR 10
Match: AT3G18720.1 (F-box family protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.1e-07
Identity = 38/126 (30.16%), Postives = 59/126 (46.83%), Query Frame = 0


Query: 3   DLPKDVMEKIGLNLNSTDYIHASVVCKSWLSLLQSDISRPQIPWILLPHQS----DDHSL 62
           DL ++++ ++GL  N    IHAS+VCK+WL    S       PW+  P        +   
Sbjct: 55  DLLQEILSRLGLKAN----IHASLVCKTWLKEAVSVRKFQSRPWLFYPQSQRGGPKEGDY 114

Query: 63  KFFNFLEVKYHILRLPKGLEG--GYIFGSSKGWLVVVFRRRRRSGWYHVYLLNPISGVKC 122
             FN    + H L+ P+ L G    +  +  GWL+VV     +     V+ LNP +G + 
Sbjct: 115 VLFNPSRSQTHHLKFPE-LTGYRNKLACAKDGWLLVV-----KDNPDVVFFLNPFTGERI 170

The following BLAST results are available for this feature:

BLAST of MC04g0329 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FVS1	8.1e-08	29.41	F-box/kelch-repeat protein At1g57790 OS=Arabidopsis thaliana OX=3702 GN=At1g5779...	[more]
Q9LSA5	1.5e-06	30.16	F-box/kelch-repeat protein At3g18720 OS=Arabidopsis thaliana OX=3702 GN=At3g1872...	[more]

BLAST of MC04g0329 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155350.1	5.15e-31	49.22	uncharacterized protein LOC111022492 [Momordica charantia]	[more]
KAE8008804.1	9.30e-26	46.09	hypothetical protein FH972_005279 [Carpinus fangiana]	[more]
KAE8055702.1	1.41e-25	43.55	hypothetical protein FH972_012527 [Carpinus fangiana]	[more]
KAE8008846.1	1.15e-24	45.53	hypothetical protein FH972_005319 [Carpinus fangiana]	[more]
KAE8008803.1	2.18e-24	45.31	hypothetical protein FH972_005278 [Carpinus fangiana]	[more]

BLAST of MC04g0329 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DM79	2.49e-31	49.22	uncharacterized protein LOC111022492 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A5N6QP56	4.50e-26	46.09	F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005279 P...	[more]
A0A5N6R432	6.81e-26	43.55	F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_012527 P...	[more]
A0A5N6QPU6	5.54e-25	45.53	F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005319 P...	[more]
A0A5N6QRN4	1.06e-24	45.31	F-box domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_005278 P...	[more]

BLAST of MC04g0329 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G57790.1	5.7e-09	29.41	F-box family protein	[more]
AT3G18720.1	1.1e-07	30.16	F-box family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001810	F-box domain	SMART	SM00256	fbox_2	coord: 4..44 e-value: 6.2E-4 score: 29.1
IPR001810	F-box domain	PFAM	PF00646	F-box	coord: 1..40 e-value: 8.0E-6 score: 25.6
IPR001810	F-box domain	PROSITE	PS50181	FBOX	coord: 1..44 score: 9.497469
None	No IPR available	GENE3D	1.20.1280.50		coord: 1..38 e-value: 7.9E-7 score: 30.8
None	No IPR available	PANTHER	PTHR44259:SF65	BNAC03G14360D PROTEIN	coord: 1..121
None	No IPR available	PANTHER	PTHR44259	OS07G0183000 PROTEIN-RELATED	coord: 1..121
IPR036047	F-box-like domain superfamily	SUPERFAMILY	81383	F-box domain	coord: 1..39

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0329.1	MC04g0329.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding