MC04g0060 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC04g0060
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	taxadiene 5-alpha hydroxylase
Location	MC04: 460172 .. 462110 (-)
RNA-Seq Expression	MC04g0060
Synteny	MC04g0060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGTTGCCACTGTTCTCCATTTTAGCTGCAATACTAATTATGTTCATTTTTCTCTTTTCAAAACCGAAAAGAAGCAGTAACGGAAGCAAGAAGCTACCACCTGGGCAAATGGGTGTTCCCTGGATTGGAGAAACAATGGATTTTTACAGAGCCCAACGGAACAACAGATTGTTCGAGGATTTTGTTGGACCTCGAGTGGCTAAATATGGCAAAATGTTCAAAACAAGCTTGATGGGGTCGCCAACAGTGATCGTAAATGGAGCTGAAGCCAACAGATTCTTCTTGTCCAATGAGTTCAAGTTGGTGGTAAGCTCGTGGCCGTCGGCCTCAGTTCAGTTGATGGGCAAAGAATGCATCATGCAGAAGCAAGGTGAAACACACCGCTGCCTACGTGGGCTTATTGGCGCAAGCCTTGCAAGTTCTTGTCTCGAGGCTATGATGCCTAAACTGTGCACTACGATCCAGTTTCATCTCGCCACCACATGGCTTGGCCATGACACAATTGCTCTGTTCCACTCCGCAAAAGTCTTGACATTTACAATAGTGTTCGAGTGTTTGTTGGGTATCAAAGTAGAGCCAGATGTGTTGACGACGTTCGAGAGAGTTTTGGAAGGAGTTTTTGCACCACCAATCAGGTTGATAAACTATCGTTGAACCCAAAAATTGTAAATTTAGAATACGGGATCTTCTACTTTCATACCAAATTAAATTATCGTTGAGTTCAAAAGTTAAAACAGATAGATTAGGATAGATTTAATCATTTATTCATATTCTTAAGACAGATTTCCAGGCTCAAGATTTTTGAGAGCCAAGAAAGCAAGGTTAGAGATAGAGAAAATGCTGAGGAAAGTGGTGAGAGAGAAGAGGAGGAAAATTGAAGGAAAGCTAGAAGAAGAAGAAGAGGGTGGATCATTGCTTTCAAGGATGGTGATGGCGATGATAAGGGGGGAGATTAGTGAAGTGGAAGTCATAGACAACGTGGTTTTGCTTGTTTTTGCAGCCCACGATACAACCTCGTTTGCCATTGCCATGGCTTTCAAAATGTTGGCTCACCATACAGACTGCTACACACTTCTTCTTCGAGGTAACCTTGCCTGCTTGCTGTATTTTTTGTGCCGTAGAAAAAGAAAAAGTTATGTTTCGTGATTCATCAACACTTCATAATAATATGAAATATTTTAACTATGTGATTAGTTTTTTAATATAGTGAGCTGCAAAAACAATTTTGATGATTCGAGTGGGAGTTAAGATGTATAGAATTGTAAAGAAAAGTGCCTTTACTTCTTTATAGAACACGATGACATACTGAGAAGTAAGGGAGAAGACCAGAATTTGACGATGGAAGATATAAAGAAGATGAAGTATACTTGGCAGGTAGCTCGTGAGACCATGCGGCTGTTTCCTCCTATATTTGGTTCTTTCAGGAAAGCGATTTCAGACATTGAATACGAAGGATTCTTCATTCCAAAAGGATGGAAGGTGAAGATGCCATTAATCCTTCTGCTTCTTCTTTTAATGAACTGAAAATCCTGCAGTCAAATTGTAGTCCAGAAACGACTGTCCTTTTTTCCTGAAATCATCTTGGTTTCGTGCAGGTCTTGTGGACGACGTATGGAACGCATTACGAGGAAGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTCGAGGAACCTGTTTCCCAGTATGTATATGTCCCATTTGGAGGAGGGCCAAGAGCTTGTGCGGGCTACCAGCTAGCCAAATTGAACATCCTCATCTTTATACACTTTGTTGTGACTCGTTATAATTGGAGTTTAATCCATCCGGACGAACCAATCATAATGGATCCTCTCCCTTTCCCTTCTCATGGAATGCCAATCAAGATATCTCCAAAATCAGAAGTTTGTACCAATATACAGCAAATGTAGTCTCATTTCTCTCTCTATGACAA

mRNA sequence

CTGTTGCCACTGTTCTCCATTTTAGCTGCAATACTAATTATGTTCATTTTTCTCTTTTCAAAACCGAAAAGAAGCAGTAACGGAAGCAAGAAGCTACCACCTGGGCAAATGGGTGTTCCCTGGATTGGAGAAACAATGGATTTTTACAGAGCCCAACGGAACAACAGATTGTTCGAGGATTTTGTTGGACCTCGAGTGGCTAAATATGGCAAAATGTTCAAAACAAGCTTGATGGGGTCGCCAACAGTGATCGTAAATGGAGCTGAAGCCAACAGATTCTTCTTGTCCAATGAGTTCAAGTTGGTGGTAAGCTCGTGGCCGTCGGCCTCAGTTCAGTTGATGGGCAAAGAATGCATCATGCAGAAGCAAGGTGAAACACACCGCTGCCTACGTGGGCTTATTGGCGCAAGCCTTGCAAGTTCTTGTCTCGAGGCTATGATGCCTAAACTGTGCACTACGATCCAGTTTCATCTCGCCACCACATGGCTTGGCCATGACACAATTGCTCTGTTCCACTCCGCAAAAGTCTTGACATTTACAATAGTGTTCGAGTGTTTGTTGGGTATCAAAGTAGAGCCAGATGTGTTGACGACGTTCGAGAGAGTTTTGGAAGGAGTTTTTGCACCACCAATCAGATTTCCAGGCTCAAGATTTTTGAGAGCCAAGAAAGCAAGGTTAGAGATAGAGAAAATGCTGAGGAAAGTGGTGAGAGAGAAGAGGAGGAAAATTGAAGGAAAGCTAGAAGAAGAAGAAGAGGGTGGATCATTGCTTTCAAGGATGGTGATGGCGATGATAAGGGGGGAGATTAGTGAAGTGGAAGTCATAGACAACGTGGTTTTGCTTGTTTTTGCAGCCCACGATACAACCTCGTTTGCCATTGCCATGGCTTTCAAAATGTTGGCTCACCATACAGACTGCTACACACTTCTTCTTCGAGAACACGATGACATACTGAGAAGTAAGGGAGAAGACCAGAATTTGACGATGGAAGATATAAAGAAGATGAAGTATACTTGGCAGGTAGCTCGTGAGACCATGCGGCTGTTTCCTCCTATATTTGGTTCTTTCAGGAAAGCGATTTCAGACATTGAATACGAAGGATTCTTCATTCCAAAAGGATGGAAGGTCTTGTGGACGACGTATGGAACGCATTACGAGGAAGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTCGAGGAACCTGTTTCCCAGTATGTATATGTCCCATTTGGAGGAGGGCCAAGAGCTTGTGCGGGCTACCAGCTAGCCAAATTGAACATCCTCATCTTTATACACTTTGTTGTGACTCGTTATAATTGGAGTTTAATCCATCCGGACGAACCAATCATAATGGATCCTCTCCCTTTCCCTTCTCATGGAATGCCAATCAAGATATCTCCAAAATCAGAAGTTTGTACCAATATACAGCAAATGTAGTCTCATTTCTCTCTCTATGACAA

Coding sequence (CDS)

CTGTTGCCACTGTTCTCCATTTTAGCTGCAATACTAATTATGTTCATTTTTCTCTTTTCAAAACCGAAAAGAAGCAGTAACGGAAGCAAGAAGCTACCACCTGGGCAAATGGGTGTTCCCTGGATTGGAGAAACAATGGATTTTTACAGAGCCCAACGGAACAACAGATTGTTCGAGGATTTTGTTGGACCTCGAGTGGCTAAATATGGCAAAATGTTCAAAACAAGCTTGATGGGGTCGCCAACAGTGATCGTAAATGGAGCTGAAGCCAACAGATTCTTCTTGTCCAATGAGTTCAAGTTGGTGGTAAGCTCGTGGCCGTCGGCCTCAGTTCAGTTGATGGGCAAAGAATGCATCATGCAGAAGCAAGGTGAAACACACCGCTGCCTACGTGGGCTTATTGGCGCAAGCCTTGCAAGTTCTTGTCTCGAGGCTATGATGCCTAAACTGTGCACTACGATCCAGTTTCATCTCGCCACCACATGGCTTGGCCATGACACAATTGCTCTGTTCCACTCCGCAAAAGTCTTGACATTTACAATAGTGTTCGAGTGTTTGTTGGGTATCAAAGTAGAGCCAGATGTGTTGACGACGTTCGAGAGAGTTTTGGAAGGAGTTTTTGCACCACCAATCAGATTTCCAGGCTCAAGATTTTTGAGAGCCAAGAAAGCAAGGTTAGAGATAGAGAAAATGCTGAGGAAAGTGGTGAGAGAGAAGAGGAGGAAAATTGAAGGAAAGCTAGAAGAAGAAGAAGAGGGTGGATCATTGCTTTCAAGGATGGTGATGGCGATGATAAGGGGGGAGATTAGTGAAGTGGAAGTCATAGACAACGTGGTTTTGCTTGTTTTTGCAGCCCACGATACAACCTCGTTTGCCATTGCCATGGCTTTCAAAATGTTGGCTCACCATACAGACTGCTACACACTTCTTCTTCGAGAACACGATGACATACTGAGAAGTAAGGGAGAAGACCAGAATTTGACGATGGAAGATATAAAGAAGATGAAGTATACTTGGCAGGTAGCTCGTGAGACCATGCGGCTGTTTCCTCCTATATTTGGTTCTTTCAGGAAAGCGATTTCAGACATTGAATACGAAGGATTCTTCATTCCAAAAGGATGGAAGGTCTTGTGGACGACGTATGGAACGCATTACGAGGAAGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTCGAGGAACCTGTTTCCCAGTATGTATATGTCCCATTTGGAGGAGGGCCAAGAGCTTGTGCGGGCTACCAGCTAGCCAAATTGAACATCCTCATCTTTATACACTTTGTTGTGACTCGTTATAATTGGAGTTTAATCCATCCGGACGAACCAATCATAATGGATCCTCTCCCTTTCCCTTCTCATGGAATGCCAATCAAGATATCTCCAAAATCAGAAGTTTGTACCAATATACAGCAAATGTAG

Protein sequence

LLPLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM

Homology

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BLAST of MC04g0060 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 349.0 bits (894), Expect = 8.7e-95
Identity = 194/478 (40.59%), Postives = 298/478 (62.34%), Query Frame = 0


Query: 6   SILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPR 65
           S +A IL++ +   SK     + S KLPPG++G+P+IGE+  F RA R+N L E F   R
Sbjct: 30  SAIAGILLLLLLFRSK----RHSSLKLPPGKLGIPFIGESFIFLRALRSNSL-EQFFDER 89

Query: 66  VAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQGE 125
           V K+G +FKTSL+G PTV++ G   NR  LSNE KLV  SWP+  ++LMG+  +  ++GE
Sbjct: 90  VKKFGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRGE 149

Query: 126 THRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFEC 185
            H  +R  +        L++ + K+ T IQ H+   W G D + +    + L F I    
Sbjct: 150 DHIVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAIL 209

Query: 186 LLGI--KVEPDVL-TTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRK 245
              I  K E D L    E +L G FA PI  PG  F RA + R ++ K++  ++++++  
Sbjct: 210 FFNIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKED 269

Query: 246 IEGKLEEEEEGGSLLSRMVMAMIRGE----ISEVEVIDNVVLLVFAAHDTTSFAIAMAFK 305
           ++       +   LLS  V+   R +    ++  E++DN   L+ A++DTT+  +A+ FK
Sbjct: 270 LQSGSATATQ--DLLS--VLLTFRDDKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFK 329

Query: 306 MLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRK 365
           +L+ + +CY  +++E  +IL +K E + +T +D+K MKYTWQVA+ET+R+FPP+FG+FRK
Sbjct: 330 LLSSNPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRK 389

Query: 366 AISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEP---VSQYVYVPFGG 425
           AI+DI+Y+G+ IPKGWK+LWTTY TH ++ YF +P  F PSRF++    V+ Y ++PFGG
Sbjct: 390 AITDIQYDGYTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGG 449

Query: 426 GPRACAGYQLAKLNILIFI-HFVVTRYNWSLIHPDEPIIMDPL-PFPSHGMPIKISPK 472
           G R+C G++ +K+ IL+F+ HFV T  +++ + PDE I  DPL P PS G  IK+ P+
Sbjct: 450 GQRSCVGWEFSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

[Close]

BLAST of MC04g0060 vs. ExPASy Swiss-Prot
Match: Q8W4T9 (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.9e-89
Identity = 181/467 (38.76%), Postives = 284/467 (60.81%), Query Frame = 0


Query: 16  IFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKT 75
           I LF + KR S  S KLPPG +G P +GET+ F R+  ++   + F+  R++K+G +FKT
Sbjct: 24  IILFFRSKRHS--SVKLPPGNLGFPLVGETLQFVRSLGSSTP-QQFIEERMSKFGDVFKT 83

Query: 76  SLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIG 135
           S++G PTV++ G   NR  LSNE KLV  SWPS+ ++L+G++C+  K GE HR +R  + 
Sbjct: 84  SIIGHPTVVLCGPAGNRLVLSNENKLVQMSWPSSMMKLIGEDCLGGKTGEQHRIVRAALT 143

Query: 136 ASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFECLLGI---KVE 195
             L    L+    K+ + IQ H+   W G D   +    K L F++      GI    ++
Sbjct: 144 RFLGPQALQNHFAKMSSGIQRHINEKWKGKDEATVLPLVKDLVFSVASRLFFGITEEHLQ 203

Query: 196 PDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKIE-GKLEEEE 255
             +    E +L G F+ P+  PG  + +A +AR  +  ++  ++ ++R ++  G   E +
Sbjct: 204 EQLHNLLEVILVGSFSVPLNIPGFSYHKAIQARATLADIMTHLIEKRRNELRAGTASENQ 263

Query: 256 EGGSLLSRMVMAMIRG-EISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLL 315
           +  S+L  +     RG  +++ E++DN  +L+  ++D+T+  + M  K+LA H + Y  +
Sbjct: 264 DLLSVL--LTFTDERGNSLADKEILDNFSMLLHGSYDSTNSPLTMLIKVLASHPESYEKV 323

Query: 316 LREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDIEYEGFFI 375
            +E   IL +K E + +  +D+K+MKY+WQV +ET+R++PPIFG+FRKAI+DI Y G+ I
Sbjct: 324 AQEQFGILSTKMEGEEIAWKDLKEMKYSWQVVQETLRMYPPIFGTFRKAITDIHYNGYTI 383

Query: 376 PKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAK 435
           PKGWK+LWTTY T  +EEYF+D   FKPSRFEE    V+ Y Y+PFGGG R C G++ AK
Sbjct: 384 PKGWKLLWTTYSTQTKEEYFKDADQFKPSRFEEEGKHVTPYTYLPFGGGMRVCPGWEFAK 443

Query: 436 LNILIFIHFVVTRYN-WSLIHPDEPIIMDPL-PFPSHGMPIKISPKS 473
           +  L+F+H  V  ++    I P+E +   PL P P +G+PIK+  +S
Sbjct: 444 METLLFLHHFVKAFSGLKAIDPNEKLSGKPLPPLPVNGLPIKLYSRS 485

[Close]

BLAST of MC04g0060 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 6.0e-88
Identity = 184/469 (39.23%), Postives = 274/469 (58.42%), Query Frame = 0


Query: 8   LAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPRVA 67
           LA+ + +  F +      +   + +PPG  G P IGET++F   QR      DF   R  
Sbjct: 27  LASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRTQ 86

Query: 68  KYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQGETH 127
           KYG +F TSL+G PTV+    E NRF  SNE KLVV+SWPS SV  + +  ++   G+  
Sbjct: 87  KYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGDDA 146

Query: 128 RCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFECLL 187
           + LR ++   L    L   + ++ +  + HLA  W+G D +      K  TF++  +   
Sbjct: 147 KRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLFA 206

Query: 188 GIKVEPD---VLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKIE 247
            I  + D   +   F   ++GV   PI  PG+R+ + K A   I + L  ++ E++  +E
Sbjct: 207 SINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIALE 266

Query: 248 -GKLEEEEEGGSLLSRMVMAMIRGE-ISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAH 307
            G    E++  S L   V    +GE +++ E+ DN++LL++A HDT+S  + +  K LA 
Sbjct: 267 AGNASPEQDLLSFLLSNVDE--QGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAE 326

Query: 308 HTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISD 367
           +  CY  +LRE  +I  SK E Q L  ED+++MKY+W+VA+E +RLFP + GSFRKAI +
Sbjct: 327 NPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIKE 386

Query: 368 IEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACA 427
             Y+GF IPKGWK+ WT   TH + EYF +P  F PSRFE E    Y +VPFGGGPR C 
Sbjct: 387 FIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGPPPYTFVPFGGGPRMCP 446

Query: 428 GYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISP 471
           G + A++ ILIF+H +V  +NW+L++P E +I+DP+P P +G+PIK+ P
Sbjct: 447 GNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

[Close]

BLAST of MC04g0060 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.3e-87
Identity = 185/473 (39.11%), Postives = 273/473 (57.72%), Query Frame = 0


Query: 8   LAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPRVA 67
           LA+ + +  F +      +   + +PPG  G P IGET++F   QR      DF   R  
Sbjct: 27  LASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRTQ 86

Query: 68  KYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQGETH 127
           KYG +F TSL+G PTV+    E NRF  SNE KLVV+SWPS SV  + +  ++   G+  
Sbjct: 87  KYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGDDA 146

Query: 128 RCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFECLL 187
           + LR ++   L    L   + ++ +  + HLA  W+G D +      K  TF++  +   
Sbjct: 147 KRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLFA 206

Query: 188 GIKVEPD---VLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKIE 247
            I  + D   +   F   ++GV   PI  PG+R+ +AK A   I + L  ++ E++  +E
Sbjct: 207 SINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIGLE 266

Query: 248 -GKLEEEEEGGSLLSRMVMAMIRGE-ISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAH 307
            G    E++  S L   V    +GE +++ E+ DN++LL++A HDT+S  + +  K LA 
Sbjct: 267 AGNASPEQDLLSFLLSNVDE--QGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAE 326

Query: 308 HTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISD 367
           +  CY  +LRE  DI  SK   Q L  ED+++MKY+W+VA+E +RLFP   GSFRKAI +
Sbjct: 327 NPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIKE 386

Query: 368 IEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACA 427
             Y+GF IPKGWK+ WT   TH + EYF +P +F PSRFE E    Y +VPFGGGPR C 
Sbjct: 387 FIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGPPPYTFVPFGGGPRMCP 446

Query: 428 GYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEV 475
           G + A+L IL+F+H +V    W+L++P E +I+DP+P P +G+PIK+ P   V
Sbjct: 447 GNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDSV 495

[Close]

BLAST of MC04g0060 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 4.3e-86
Identity = 185/481 (38.46%), Postives = 291/481 (60.50%), Query Frame = 0


Query: 4   LFSILAAI--LIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDF 63
           LF +L+A+  +++ + LF + KR S  S  LPPG++G P+IGE++ F +A R+N + E F
Sbjct: 22  LFIVLSAVAGIVLPLLLFLRSKRRS--SVGLPPGKLGYPFIGESLLFLKALRSNTV-EQF 81

Query: 64  VGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQ 123
           +  RV  +G +FKTSL+G PTV++ G   NR  L+NE KLV  SWP +S++LMG++ I  
Sbjct: 82  LDERVKNFGNVFKTSLIGHPTVVLCGPAGNRLILANEEKLVQMSWPKSSMKLMGEKSITA 141

Query: 124 KQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTI 183
           K+GE H  +R  +    +   L+  + ++  TI+ H+   W G+D +++      L F I
Sbjct: 142 KRGEGHMIIRSALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDI 201

Query: 184 VFECLLGIKVEPD---VLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVRE 243
                  I  + +   +    E +  GV A P+  PG  + RA +AR ++  +L  ++ +
Sbjct: 202 SACLFFNINEKHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEK 261

Query: 244 KRRKIEGKLEEEEEGGSLLS-RMVMAMIRGE-ISEVEVIDNVVLLVFAAHDTTSFAIAMA 303
           ++  +   L    +   LLS  +     RG   S+ E++DN   L+  ++DTT  A+A  
Sbjct: 262 RKMDLSSGLATSNQ--DLLSVFLTFKDDRGNPCSDEEILDNFSGLLHGSYDTTVSAMACV 321

Query: 304 FKMLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSF 363
           FK+L+ + +CY  +++E   IL +K E   +T +D+K MKYTWQV +ET+RL+P IFGSF
Sbjct: 322 FKLLSSNPECYEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFGSF 381

Query: 364 RKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEP---VSQYVYVPF 423
           R+AI+DI Y G+ IPKGWK+LWT Y TH +E YF +P  F PSRF++    V+ Y ++PF
Sbjct: 382 RQAITDIHYNGYIIPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQEGKLVAPYTFLPF 441

Query: 424 GGGPRACAGYQLAKLNILIFI-HFVVTRYNWSLIHPDEPIIMDPL-PFPSHGMPIKISPK 473
           GGG R+C G++ +K+ IL+ + HFV T   ++ + P E I  D L P PS+G  +K+ P+
Sbjct: 442 GGGQRSCPGWEFSKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSNGFSVKLFPR 497

[Close]

BLAST of MC04g0060 vs. NCBI nr
Match: XP_022147913.1 (taxadiene 5-alpha hydroxylase isoform X1 [Momordica charantia])

HSP 1 Score: 955 bits (2469), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0


Query: 1   LLPLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFED 60
           LLPLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFED
Sbjct: 9   LLPLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFED 68

Query: 61  FVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIM 120
           FVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIM
Sbjct: 69  FVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIM 128

Query: 121 QKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFT 180
           QKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFT
Sbjct: 129 QKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFT 188

Query: 181 IVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKR 240
           IVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKR
Sbjct: 189 IVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKR 248

Query: 241 RKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKML 300
           RKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKML
Sbjct: 249 RKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKML 308

Query: 301 AHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAI 360
           AHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAI
Sbjct: 309 AHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAI 368

Query: 361 SDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRAC 420
           SDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRAC
Sbjct: 369 SDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRAC 428

Query: 421 AGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQ 480
           AGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQ
Sbjct: 429 AGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQ 488

[Close]

BLAST of MC04g0060 vs. NCBI nr
Match: XP_022147914.1 (taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia] >XP_022147915.1 taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia] >XP_022147916.1 taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia] >XP_022147917.1 taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia])

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0


Query: 37  MGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLS 96
           MGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLS
Sbjct: 1   MGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLS 60

Query: 97  NEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQF 156
           NEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQF
Sbjct: 61  NEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQF 120

Query: 157 HLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGS 216
           HLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGS
Sbjct: 121 HLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGS 180

Query: 217 RFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVID 276
           RFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVID
Sbjct: 181 RFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVID 240

Query: 277 NVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMK 336
           NVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMK
Sbjct: 241 NVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMK 300

Query: 337 YTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSF 396
           YTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSF
Sbjct: 301 YTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSF 360

Query: 397 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDP 456
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDP
Sbjct: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDP 420

Query: 457 LPFPSHGMPIKISPKSEVCTNIQQM 481
           LPFPSHGMPIKISPKSEVCTNIQQM
Sbjct: 421 LPFPSHGMPIKISPKSEVCTNIQQM 445

[Close]

BLAST of MC04g0060 vs. NCBI nr
Match: XP_023554746.1 (taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 786 bits (2029), Expect = 1.14e-283
Identity = 400/478 (83.68%), Postives = 434/478 (90.79%), Query Frame = 0


Query: 5   FSILAAILIM-FIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVG 64
           F  L+AILI+  IF+F    RS  GSKK+PPGQMG+PWIGETM FYRAQR NRLFE+FVG
Sbjct: 14  FLFLSAILIISIIFVF----RSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVG 73

Query: 65  PRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQ 124
           PRVAKYGK+FKTSLMGSPTV+VNGAEANRFFLSNEFKLVVSSWPSASVQLMG+ECIMQKQ
Sbjct: 74  PRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQ 133

Query: 125 GETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVF 184
           GE+HRC+RGLI ASLASS LEAMMPKLCTTIQ +L T WLGHD I+L+HSAKVLTFTIVF
Sbjct: 134 GESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVF 193

Query: 185 ECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKI 244
           ECL+GIKVEP +LTTFERVLEGVFAPP  FPG+RF RAKKAR+EI KMLR+VVREKRR+I
Sbjct: 194 ECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRI 253

Query: 245 EGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAHH 304
           E +  EEEE   LLSRMV AMIRGEISEVEV+DNVVLLVFAAHDTTSFAIAM FKMLA  
Sbjct: 254 EEE-PEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQ 313

Query: 305 TDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDI 364
            +CY+LLL+EH DI+RSKG  QN+TMEDIKKMKYTWQVARE++RLFPPIFGSFRKAI+DI
Sbjct: 314 PNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDI 373

Query: 365 EYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY 424
           EYEGFFIPKGWKVLWTTYGTH E+EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY
Sbjct: 374 EYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY 433

Query: 425 QLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM 481
           QLAKLNILIFIH VVTRY+WSL+HPDE IIMDPLP PSHGMPIKISPKSE   N+QQM
Sbjct: 434 QLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 486

[Close]

BLAST of MC04g0060 vs. NCBI nr
Match: XP_023554747.1 (taxadiene 5-alpha hydroxylase isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 782 bits (2020), Expect = 1.71e-282
Identity = 391/458 (85.37%), Postives = 422/458 (92.14%), Query Frame = 0


Query: 24  RSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTV 83
           RS  GSKK+PPGQMG+PWIGETM FYRAQR NRLFE+FVGPRVAKYGK+FKTSLMGSPTV
Sbjct: 18  RSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTV 77

Query: 84  IVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCL 143
           +VNGAEANRFFLSNEFKLVVSSWPSASVQLMG+ECIMQKQGE+HRC+RGLI ASLASS L
Sbjct: 78  VVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSL 137

Query: 144 EAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVL 203
           EAMMPKLCTTIQ +L T WLGHD I+L+HSAKVLTFTIVFECL+GIKVEP +LTTFERVL
Sbjct: 138 EAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVL 197

Query: 204 EGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMA 263
           EGVFAPP  FPG+RF RAKKAR+EI KMLR+VVREKRR+IE +  EEEE   LLSRMV A
Sbjct: 198 EGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE-PEEEECELLLSRMVRA 257

Query: 264 MIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLREHDDILRSKGE 323
           MIRGEISEVEV+DNVVLLVFAAHDTTSFAIAM FKMLA   +CY+LLL+EH DI+RSKG 
Sbjct: 258 MIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGA 317

Query: 324 DQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGT 383
            QN+TMEDIKKMKYTWQVARE++RLFPPIFGSFRKAI+DIEYEGFFIPKGWKVLWTTYGT
Sbjct: 318 GQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGT 377

Query: 384 HYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNW 443
           H E+EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIH VVTRY+W
Sbjct: 378 HLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDW 437

Query: 444 SLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM 481
           SL+HPDE IIMDPLP PSHGMPIKISPKSE   N+QQM
Sbjct: 438 SLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 474

[Close]

BLAST of MC04g0060 vs. NCBI nr
Match: XP_038888983.1 (taxadiene 5-alpha hydroxylase [Benincasa hispida])

HSP 1 Score: 781 bits (2017), Expect = 5.08e-282
Identity = 397/477 (83.23%), Postives = 431/477 (90.36%), Query Frame = 0


Query: 6   SILAAILIMFI-FLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGP 65
           S  AA+LI+ I F+    KRS N    +PPGQMG+PWIGETM FYRAQ  NRLFEDFV P
Sbjct: 3   SFFAAMLIISIAFVIRSKKRSKN----VPPGQMGLPWIGETMAFYRAQSKNRLFEDFVEP 62

Query: 66  RVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQG 125
           RVAKYGK+FKTSLMGSPTV+VNGAEANRFFLSNEFKLVVSSWPSASVQLMG+ECIMQKQG
Sbjct: 63  RVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQG 122

Query: 126 ETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFE 185
           E+HR +R LI ASL++S LE MMPKL TTI+ +L T W+G DTI LFHSAKVLTFT+VFE
Sbjct: 123 ESHRFVRSLIAASLSTSSLEVMMPKLFTTIRVYLDTKWVGRDTIGLFHSAKVLTFTVVFE 182

Query: 186 CLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKIE 245
           CL+GIKVEP VLTTFERVLEGVFAPPIRFPG+RF RAKKARLEIEKMLR+VVREKRR+IE
Sbjct: 183 CLMGIKVEPRVLTTFERVLEGVFAPPIRFPGTRFSRAKKARLEIEKMLREVVREKRRRIE 242

Query: 246 GKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAHHT 305
           GKLEEEEE GSLLSRMV AMIRGEISE+EVIDNVVL VFAAHDTTSFAIAM FKMLAHHT
Sbjct: 243 GKLEEEEECGSLLSRMVRAMIRGEISEMEVIDNVVLFVFAAHDTTSFAIAMTFKMLAHHT 302

Query: 306 DCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDIE 365
           +CY+LLL+E  DI RSKG +QNLTMEDIKKMKYTWQVARE++RLFPPIFGSFRKAISDIE
Sbjct: 303 NCYSLLLQEQTDIARSKGSNQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 362

Query: 366 YEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQ 425
           YE FFIP+GWKVLWTTYGTHY+EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQ
Sbjct: 363 YERFFIPEGWKVLWTTYGTHYKEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQ 422

Query: 426 LAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM 481
           LAKLNILIFIH VVTRY+W+L+HPDEPIIMDPLP PS GMP+KIS KS+VC N  QM
Sbjct: 423 LAKLNILIFIHLVVTRYDWNLVHPDEPIIMDPLPVPSQGMPMKISLKSDVCPNDHQM 475

[Close]

BLAST of MC04g0060 vs. ExPASy TrEMBL
Match: A0A6J1D2L6 (taxadiene 5-alpha hydroxylase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016729 PE=3 SV=1)

HSP 1 Score: 955 bits (2469), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0


Query: 1   LLPLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFED 60
           LLPLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFED
Sbjct: 9   LLPLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFED 68

Query: 61  FVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIM 120
           FVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIM
Sbjct: 69  FVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIM 128

Query: 121 QKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFT 180
           QKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFT
Sbjct: 129 QKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFT 188

Query: 181 IVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKR 240
           IVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKR
Sbjct: 189 IVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKR 248

Query: 241 RKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKML 300
           RKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKML
Sbjct: 249 RKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKML 308

Query: 301 AHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAI 360
           AHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAI
Sbjct: 309 AHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAI 368

Query: 361 SDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRAC 420
           SDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRAC
Sbjct: 369 SDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRAC 428

Query: 421 AGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQ 480
           AGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQ
Sbjct: 429 AGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQ 488

[Close]

BLAST of MC04g0060 vs. ExPASy TrEMBL
Match: A0A6J1D1G7 (taxadiene 5-alpha hydroxylase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016729 PE=3 SV=1)

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0


Query: 37  MGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLS 96
           MGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLS
Sbjct: 1   MGVPWIGETMDFYRAQRNNRLFEDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLS 60

Query: 97  NEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQF 156
           NEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQF
Sbjct: 61  NEFKLVVSSWPSASVQLMGKECIMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQF 120

Query: 157 HLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGS 216
           HLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGS
Sbjct: 121 HLATTWLGHDTIALFHSAKVLTFTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGS 180

Query: 217 RFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVID 276
           RFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVID
Sbjct: 181 RFLRAKKARLEIEKMLRKVVREKRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVID 240

Query: 277 NVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMK 336
           NVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMK
Sbjct: 241 NVVLLVFAAHDTTSFAIAMAFKMLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMK 300

Query: 337 YTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSF 396
           YTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSF
Sbjct: 301 YTWQVARETMRLFPPIFGSFRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSF 360

Query: 397 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDP 456
           KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDP
Sbjct: 361 KPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDP 420

Query: 457 LPFPSHGMPIKISPKSEVCTNIQQM 481
           LPFPSHGMPIKISPKSEVCTNIQQM
Sbjct: 421 LPFPSHGMPIKISPKSEVCTNIQQM 445

[Close]

BLAST of MC04g0060 vs. ExPASy TrEMBL
Match: A0A6J1HZM9 (taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468378 PE=3 SV=1)

HSP 1 Score: 781 bits (2016), Expect = 3.36e-282
Identity = 398/478 (83.26%), Postives = 432/478 (90.38%), Query Frame = 0


Query: 5   FSILAAILIM-FIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVG 64
           F  L+AILI+  IF+F    RS  GSKK+PPGQMG+PWIG+TM FYRAQR NRLFEDFVG
Sbjct: 2   FLFLSAILIISIIFVF----RSIKGSKKVPPGQMGLPWIGQTMAFYRAQRKNRLFEDFVG 61

Query: 65  PRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQ 124
           PRVAKYGK+FKTSLMGSPTV++NGAEANRFFLSNEFKLVVSSWP+ASVQLMG+ECIMQKQ
Sbjct: 62  PRVAKYGKIFKTSLMGSPTVVINGAEANRFFLSNEFKLVVSSWPAASVQLMGEECIMQKQ 121

Query: 125 GETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVF 184
           GE+HRC+RGLI ASLASS LEAMMPKLCTTIQ HL T W+GHD I+L+HSAKVLTF+IVF
Sbjct: 122 GESHRCVRGLIAASLASSSLEAMMPKLCTTIQLHLDTKWVGHDIISLYHSAKVLTFSIVF 181

Query: 185 ECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKI 244
           ECL+GIKVEP +LTTFERVLEGVFA P  FPG+RF RAKKAR+EI KMLR+VVREKRR+I
Sbjct: 182 ECLMGIKVEPRMLTTFERVLEGVFALPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRI 241

Query: 245 EGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAHH 304
           E +LEEEE    LLSRMV AMIRGEISEVEV+DNVVLLVFAAHDTTSFAIAM FKMLA H
Sbjct: 242 EEELEEEE-CELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQH 301

Query: 305 TDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDI 364
             CY+LLL+EH DI+RSKG  QNLTMEDIKKMKYTWQVARE++RLFPPIFGSFRKAI+DI
Sbjct: 302 PSCYSLLLQEHADIVRSKGAGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDI 361

Query: 365 EYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY 424
           EYEGFFIPKGWKVLWT YGTH E+EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY
Sbjct: 362 EYEGFFIPKGWKVLWTMYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY 421

Query: 425 QLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM 481
           QLAKLNILIFIH VVTRY+WSLIHPDE IIMDPLP PSHGM  KISPKSEV  N+QQM
Sbjct: 422 QLAKLNILIFIHLVVTRYDWSLIHPDETIIMDPLPIPSHGMQTKISPKSEVRPNVQQM 474

[Close]

BLAST of MC04g0060 vs. ExPASy TrEMBL
Match: A0A6J1GJY9 (taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 781 bits (2017), Expect = 3.71e-282
Identity = 398/478 (83.26%), Postives = 433/478 (90.59%), Query Frame = 0


Query: 5   FSILAAILIM-FIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVG 64
           F  L+AILI+  IF+F    RS  GSKK+PPGQMG+PWIGETM FYRAQR NRLFE+FVG
Sbjct: 14  FLFLSAILIISIIFVF----RSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVG 73

Query: 65  PRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQ 124
           PRVAKYGK+FKTSLMGSPTV+VNGAEANRFFLSNEFKLVVSSWPSASVQLMG+ECIMQKQ
Sbjct: 74  PRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQ 133

Query: 125 GETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVF 184
           GE+HRC+RGLI ASLASS LEAMMPKLCTTIQ +L T WLGHD I+L+HSAKVLTFTIVF
Sbjct: 134 GESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVF 193

Query: 185 ECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKI 244
           ECL+GIKVEP +LTTFERVLEGVFAPP  FPG+RF RAKKAR+EI KMLR+VVREKRR+I
Sbjct: 194 ECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRI 253

Query: 245 EGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAHH 304
           E +   EEE   LLSRMV AMIRGEISEVEV+DNVVLLVFAAHDTTSFAIAM FKMLA  
Sbjct: 254 EEE-PVEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQ 313

Query: 305 TDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDI 364
            +CY+LLL+EH DI+RSKG  QN+TMEDIKKMKYTWQVARE++RLFPPIFGSFRKAI+DI
Sbjct: 314 PNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDI 373

Query: 365 EYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY 424
           EYEGFFIPKGWKVLWTTYGTH E++YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY
Sbjct: 374 EYEGFFIPKGWKVLWTTYGTHLEDKYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY 433

Query: 425 QLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM 481
           QLAKLNILIFIH VVTRY+WSL+HPDE IIMDPLP PSHGMPIKISPKSE   N+QQM
Sbjct: 434 QLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 486

[Close]

BLAST of MC04g0060 vs. ExPASy TrEMBL
Match: A0A1S3BLK9 (taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1)

HSP 1 Score: 779 bits (2012), Expect = 1.47e-281
Identity = 390/476 (81.93%), Postives = 427/476 (89.71%), Query Frame = 0


Query: 6   SILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPR 65
           S LAAIL++    F    RS+  SK +PPG+MG+PWIGETM FYRAQ  NRLFEDFV PR
Sbjct: 3   SFLAAILLIITIAFVL--RSNKRSKNVPPGEMGLPWIGETMAFYRAQSKNRLFEDFVEPR 62

Query: 66  VAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQGE 125
           VAKYGK+FKT+LMGSPTV+VNGAEANRFFLSNEFKLVVSSWPS+SVQLMG+ECIMQK+GE
Sbjct: 63  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 122

Query: 126 THRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFEC 185
            HRC+RGLI ++L++S LE MMPKLC TIQ +L T W+  DT+ LF SAKVLTFTIVFEC
Sbjct: 123 KHRCVRGLIASALSTSSLEVMMPKLCNTIQVYLDTKWVDCDTVGLFRSAKVLTFTIVFEC 182

Query: 186 LLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRKIEG 245
           LLGIKVEP VLTTFERVLEGVFAPP RFPG+RF RAKKARLEIE MLR+VVREKRRKIEG
Sbjct: 183 LLGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIETMLREVVREKRRKIEG 242

Query: 246 KLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKMLAHHTD 305
           KLEEEE+ GSLLSRMV AMI GEISE+EV+DN+VLLVFAAHDTTSFAIAM FKML+ HT+
Sbjct: 243 KLEEEEQCGSLLSRMVRAMIGGEISEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTN 302

Query: 306 CYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKAISDIEY 365
           CY+LLL+EH DI+RSKG  QNLTMEDIKKMKYTWQVARE++RLFPPIFGSFRKAISDIEY
Sbjct: 303 CYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY 362

Query: 366 EGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 425
           E F IPKGWKVLWTTYGTHY EEYF DPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL
Sbjct: 363 ESFLIPKGWKVLWTTYGTHYGEEYFGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 422

Query: 426 AKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPKSEVCTNIQQM 481
           AKLNILIFIH VVTRY+WSL+HPDEPIIMDPLP P HGMPIKISPKSE+C N+ QM
Sbjct: 423 AKLNILIFIHSVVTRYDWSLVHPDEPIIMDPLPVPCHGMPIKISPKSEICPNVHQM 476

[Close]

BLAST of MC04g0060 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 609.0 bits (1569), Expect = 3.3e-174
Identity = 299/473 (63.21%), Postives = 372/473 (78.65%), Query Frame = 0


Query: 4   LFSILAAILIMFIFLFSKP-----KRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLF 63
           +F  +AA +   +F F K       +     KKL PG+MG+PWIGETMDFY+AQ++NR+F
Sbjct: 12  VFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVF 71

Query: 64  EDFVGPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKEC 123
           EDFV PR+ K+G +FKT +MGSPT++VNGAEANR  LSNEF LVVSSWPS+SVQLMG  C
Sbjct: 72  EDFVNPRIIKHGNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNC 131

Query: 124 IMQKQGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLT 183
           IM KQGE HR LRG++  SL+   LE+++PKLC T++FH  T W G + I+L+ SAKVLT
Sbjct: 132 IMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLT 191

Query: 184 FTIVFECLLGIKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVRE 243
           FT+VFECL GIKVE  +L  FERVLEGVFA P+ FP S+F RAKKARLEIE  L   VRE
Sbjct: 192 FTVVFECLYGIKVEIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVRE 251

Query: 244 KRRKIEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFK 303
           KRR++E K   E+   +L SR+V  +I+G I+E EV+DN+VLLVFAAHDTTS+A++M FK
Sbjct: 252 KRREME-KEGAEKPNTTLFSRLVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFK 311

Query: 304 MLAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRK 363
           MLA H  C   LL+EH  I  +KGE + LT+ED+KKMKY+WQV RETMRL PPIFGSFRK
Sbjct: 312 MLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRK 371

Query: 364 AISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPR 423
           A++DI+Y G+ IPKGWK+LWTTYGTHY  E F+DP+SF P+RF++P+  Y Y+PFGGGPR
Sbjct: 372 AVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKPIQAYTYLPFGGGPR 431

Query: 424 ACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPK 472
            CAG+QLAK++IL+F+HFVVT ++WSL++PDE I MDPLPFPS GMPIKISPK
Sbjct: 432 LCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483

[Close]

BLAST of MC04g0060 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 305.1 bits (780), Expect = 1.0e-82
Identity = 181/475 (38.11%), Postives = 260/475 (54.74%), Query Frame = 0


Query: 8   LAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPRVA 67
           L++IL+  + L  K   S      LPPG  G+P IGE+  F  A R     E F+  RV 
Sbjct: 11  LSSILLSLLLLLRK-HLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHP-EKFITDRVR 70

Query: 68  KYGK----MFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQ 127
           ++      +FKT L GSPT +V GA  N+F  +NE KLVVS WP  SV  +    +    
Sbjct: 71  RFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPD-SVNKIFPSSMQTSS 130

Query: 128 GETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVF 187
            E  R LR L+   +    L   +  +    Q H  T W   D + +F   K  TF+I  
Sbjct: 131 KEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFTFSIAC 190

Query: 188 ECLLGIKVEPDVL---TTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKR 247
              L ++    V      F  V  G+F+ PI  PG+RF RA KA   + K +  +VR+++
Sbjct: 191 RSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAIVRQRK 250

Query: 248 RKIE-GKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKM 307
            +++ GK  EE +   +LS M+M +  GE  + ++ D ++ L+   HDT S         
Sbjct: 251 EELKAGKALEEHD---ILSHMLMNI--GETKDEDLADKIIGLLIGGHDTASIVCTFVVNY 310

Query: 308 LAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKA 367
           LA     Y  +L+E  +IL+ K E + L  EDI+KM+Y+W VA E MR+ PP+ G+FR+A
Sbjct: 311 LAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFREA 370

Query: 368 ISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPR 427
           I    ++GF+IPKGWK+ W+   TH   +YF +P  F+P+RFE      Y YVPFGGGPR
Sbjct: 371 IDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPFGGGPR 430

Query: 428 ACAGYQLAKLNILIFIHFVVTRYNWSLIHPDE-PIIMDPLPFPSHGMPIKISPKS 473
            C G + A+L ILIF+H +V R+ W  + P+E  I++DPLP P  G+PI+I P+S
Sbjct: 431 MCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQS 477

[Close]

BLAST of MC04g0060 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-69
Identity = 156/475 (32.84%), Postives = 249/475 (52.42%), Query Frame = 0


Query: 3   PLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFV 62
           P    L  ++++ + LF           +LPPG MG+P+IGET+  Y    N+     F 
Sbjct: 20  PALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FF 79

Query: 63  GPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQK 122
             R  KYG +FKT ++G P V+++  EA R  L ++  L   ++P +  +++G E +   
Sbjct: 80  ATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFH 139

Query: 123 QGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIV 182
           QG  H  L+ L+ +S   S L   +  +   +     ++W    +I      K   F + 
Sbjct: 140 QGPYHSTLKRLVQSSFMPSALRPTVSHI-ELLVLQTLSSWTSQKSINTLEYMKRYAFDVA 199

Query: 183 FECLLGIKVEPDVLTT----FERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVRE 242
                G K EP  +      ++R+  G  + P+  PG+ F ++ KAR+E+ + LRKV+ E
Sbjct: 200 IMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI-E 259

Query: 243 KRRKIEGKLEEEEEGGSLLSRMVMA--MIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMA 302
           KRR      E   E G LL  ++ A    R  +S+ ++ DN++ ++FAA DTT+  +   
Sbjct: 260 KRR------ENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWL 319

Query: 303 FKMLAHHTDCYTLLLREHDDILRS-KGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGS 362
            K L  H +    + RE   I +  K E++ ++ ED +KM  T +V +ET+R    +  +
Sbjct: 320 LKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFT 379

Query: 363 FRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGG 422
           FR+A+ D+EY+G+ IPKGWKVL      H+  E+F DP  F PSRFE     Y Y+PFG 
Sbjct: 380 FREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGN 439

Query: 423 GPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISP 471
           G  +C G +LAKL +LI +H + T + W +I  +E I   P P P  G+PI+++P
Sbjct: 440 GVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of MC04g0060 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-69
Identity = 156/475 (32.84%), Postives = 249/475 (52.42%), Query Frame = 0


Query: 3   PLFSILAAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFV 62
           P    L  ++++ + LF           +LPPG MG+P+IGET+  Y    N+     F 
Sbjct: 20  PALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FF 79

Query: 63  GPRVAKYGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQK 122
             R  KYG +FKT ++G P V+++  EA R  L ++  L   ++P +  +++G E +   
Sbjct: 80  ATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFH 139

Query: 123 QGETHRCLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIV 182
           QG  H  L+ L+ +S   S L   +  +   +     ++W    +I      K   F + 
Sbjct: 140 QGPYHSTLKRLVQSSFMPSALRPTVSHI-ELLVLQTLSSWTSQKSINTLEYMKRYAFDVA 199

Query: 183 FECLLGIKVEPDVLTT----FERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVRE 242
                G K EP  +      ++R+  G  + P+  PG+ F ++ KAR+E+ + LRKV+ E
Sbjct: 200 IMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI-E 259

Query: 243 KRRKIEGKLEEEEEGGSLLSRMVMA--MIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMA 302
           KRR      E   E G LL  ++ A    R  +S+ ++ DN++ ++FAA DTT+  +   
Sbjct: 260 KRR------ENGREEGGLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWL 319

Query: 303 FKMLAHHTDCYTLLLREHDDILRS-KGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGS 362
            K L  H +    + RE   I +  K E++ ++ ED +KM  T +V +ET+R    +  +
Sbjct: 320 LKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFT 379

Query: 363 FRKAISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGG 422
           FR+A+ D+EY+G+ IPKGWKVL      H+  E+F DP  F PSRFE     Y Y+PFG 
Sbjct: 380 FREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGN 439

Query: 423 GPRACAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISP 471
           G  +C G +LAKL +LI +H + T + W +I  +E I   P P P  G+PI+++P
Sbjct: 440 GVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of MC04g0060 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 248.8 bits (634), Expect = 8.7e-66
Identity = 147/472 (31.14%), Postives = 245/472 (51.91%), Query Frame = 0


Query: 9   AAILIMFIFLFSKPKRSSNGSKKLPPGQMGVPWIGETMDFYRAQRNNRLFEDFVGPRVAK 68
           AA+ +  +   +  +RSS+    LPPG MG P++GET   Y    N      F   +  +
Sbjct: 13  AALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPN-----VFFAAKQRR 72

Query: 69  YGKMFKTSLMGSPTVIVNGAEANRFFLSNEFKLVVSSWPSASVQLMGKECIMQKQGETHR 128
           YG +FKT ++G P V+++  EA +F L  +  L   ++P++  +++GK+ I   QG+ H 
Sbjct: 73  YGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHS 132

Query: 129 CLRGLIGASLASSCLEAMMPKLCTTIQFHLATTWLGHDTIALFHSAKVLTFTIVFECLLG 188
            LR L+  +     +  M+P + +  Q  L  +W G   +  +   K  TF +    +LG
Sbjct: 133 KLRKLVLRAFMPDAIRNMVPHIESIAQESL-NSWDG-TQLNTYQEMKTYTFNVALISILG 192

Query: 189 ---IKVEPDVLTTFERVLEGVFAPPIRFPGSRFLRAKKARLEIEKMLRKVVREKRRK--- 248
              +    D+   +  + +G  + PI  PG+ F +A KAR E+ ++L  ++ ++R+    
Sbjct: 193 KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSS 252

Query: 249 ---IEGKLEEEEEGGSLLSRMVMAMIRGEISEVEVIDNVVLLVFAAHDTTSFAIAMAFKM 308
              + G   E++ G               +++ ++ DN++ ++FAA DTT+  +    K 
Sbjct: 253 HTDLLGSFMEDKAG---------------LTDEQIADNIIGVIFAARDTTASVLTWILKY 312

Query: 309 LAHHTDCYTLLLREHDDILRSKGEDQNLTMEDIKKMKYTWQVARETMRLFPPIFGSFRKA 368
           LA +      +  E   I + K E ++LT ED KKM  T++V +ET+R    +  +FR+A
Sbjct: 313 LADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREA 372

Query: 369 ISDIEYEGFFIPKGWKVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRA 428
           + D+EYEG+ IPKGWKVL      H+  + F DP  F PSRFE       ++PFG G  +
Sbjct: 373 VEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHS 432

Query: 429 CAGYQLAKLNILIFIHFVVTRYNWSLIHPDEPIIMDPLPFPSHGMPIKISPK 472
           C G +LAKL I + IH + T+Y WS++ P + I   P   P +G+PI +  K
Sbjct: 433 CPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALERK 462

The following BLAST results are available for this feature:

BLAST of MC04g0060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WG30	8.7e-95	40.59	Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2	[more]
Q8W4T9	1.9e-89	38.76	Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1	[more]
Q50EK1	6.0e-88	39.23	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q50EK0	2.3e-87	39.11	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]
Q84KI1	4.3e-86	38.46	Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1	[more]

BLAST of MC04g0060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147913.1	0.0	100.00	taxadiene 5-alpha hydroxylase isoform X1 [Momordica charantia]	[more]
XP_022147914.1	0.0	100.00	taxadiene 5-alpha hydroxylase isoform X2 [Momordica charantia] >XP_022147915.1 t...	[more]
XP_023554746.1	1.14e-283	83.68	taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023554747.1	1.71e-282	85.37	taxadiene 5-alpha hydroxylase isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_038888983.1	5.08e-282	83.23	taxadiene 5-alpha hydroxylase [Benincasa hispida]	[more]

BLAST of MC04g0060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2L6	0.0	100.00	taxadiene 5-alpha hydroxylase isoform X1 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1D1G7	0.0	100.00	taxadiene 5-alpha hydroxylase isoform X2 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1HZM9	3.36e-282	83.26	taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1GJY9	3.71e-282	83.26	taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A1S3BLK9	1.47e-281	81.93	taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1	[more]

BLAST of MC04g0060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42850.1	3.3e-174	63.21	cytochrome P450, family 718	[more]
AT5G36110.1	1.0e-82	38.11	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G29090.1	2.2e-69	32.84	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	2.2e-69	32.84	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT5G45340.1	8.7e-66	31.14	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 221..241
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 4..452 e-value: 1.7E-13 score: 48.1
None	No IPR available	PANTHER	PTHR24286:SF221	TAXADIENE 5-ALPHA HYDROXYLASE	coord: 13..470
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 13..470
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 66..85 score: 26.16 coord: 379..403 score: 26.98 coord: 273..290 score: 28.88 coord: 420..443 score: 22.3 coord: 410..420 score: 50.41
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 420..431 score: 33.52 coord: 411..420 score: 58.89 coord: 340..351 score: 35.03 coord: 284..301 score: 31.11
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 33..457 e-value: 9.3E-58 score: 196.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 22..471 e-value: 1.0E-110 score: 372.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 29..470
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 413..422

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC04g0060.1	MC04g0060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen