MC03g_new0293 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0293 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 6.1e-153
Identity = 267/534 (50.00%), Postives = 369/534 (69.10%), Query Frame = 0

Query: 10  AFAFACSIVLSSSLF--WAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFSYSSILNF 69
           +F F C I+     F    + A+  E FL+C ++H P+N +  K +YT  +  Y SILN 
Sbjct: 7   SFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNS 66

Query: 70  SIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVSHLP 129
           +IQNLRF +  TPKPL+++ PS+ SHIQA ++CSK  GLQIRTRSGGHD EG+SY+S +P
Sbjct: 67  TIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVP 126

Query: 130 FIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVGVGGHLS 189
           F+V+DL ++ SI ID  + TAWV++GATLGE+YY I +K++ L+FP G CPTVGVGGH S
Sbjct: 127 FVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFS 186

Query: 190 GGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGIVVAWKL 249
           GGGYG L+R YGLAAD++IDAHLV+ +G++L+R+SM +DLFW IRGGGG +FGI+ AWK+
Sbjct: 187 GGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKI 246

Query: 250 RLVPVPTTLTTCTVNRTLDRDG-VKLIHRWQYVAHKLEDNLLLGISLTGGHILTQQG-GK 309
           +LV VP+  T  +V + ++  G VKL ++WQ +A+K + +L+L       +I    G  K
Sbjct: 247 KLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNK 306

Query: 310 TNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGF----SAGES 369
           T     F + F G VD LV ++N +FPELG+ K DC E SWI++T+  +G     +A   
Sbjct: 307 TTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFK 366

Query: 370 LETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEF 429
            E LL R      +  IK DYVK+PI E     I E+L  +D+     +  PYGG M E 
Sbjct: 367 KEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEI 426

Query: 430 SESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNY 489
           SES  P+PHRA  MY++ Y   WE+Q  +  ++H+NWVR +Y++  PYV+++PR AY+NY
Sbjct: 427 SESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNY 486

Query: 490 RDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 536
           RDLD+G+ N     NY +A++WG KYFGKNFNRLV+VKT+VDP++FFR+EQSIP
Sbjct: 487 RDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIP 538

BLAST of MC03g_new0293 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 3.9e-152
Identity = 265/537 (49.35%), Postives = 365/537 (67.97%), Query Frame = 0

Query: 2   KSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFSY 61
           +SF  + L F    +++LS     +A+      FLQCL+     +N +SK I+TP + S+
Sbjct: 4   QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSF 63

Query: 62  SSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLS 121
           SS+L  SIQN RFS  + PKP+L++ P   S +Q+AV C++  G+ IRTRSGGHD+EGLS
Sbjct: 64  SSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLS 123

Query: 122 YVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVG 181
           YV+H PF++LDL +LRSIT+D  N + WVQ+GAT+GELYY I +K++TLAFPAG CPTVG
Sbjct: 124 YVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVG 183

Query: 182 VGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGI 241
           VGGH SGGGYG L+RK+GLAADHVIDA +VDA GRIL R  M +D FW IRGGGG SF +
Sbjct: 184 VGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCV 243

Query: 242 VVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQ 301
           V++WK+ L+ VP+T+T   V +  ++  +K+IHRWQ+VA K+ D+L + + L        
Sbjct: 244 VLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------- 303

Query: 302 QGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGES 361
           Q  K    ASFP  +LG V  L+ +VN  FPELGL ++DC E SWIES +  A    GE 
Sbjct: 304 QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA--ELGEE 363

Query: 362 LETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEF 421
              +L +R   +++ K KSD+V+EP+ +     +W RL   + E AQ++F P+GG+MSE 
Sbjct: 364 PINVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 423

Query: 422 SESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNY 481
           ++ ETP+PHR  N+Y++ Y+  W       +++++ WV  +YD M+ +V KSPR AY+N 
Sbjct: 424 ADYETPFPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINL 483

Query: 482 RDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 539
           RDLD+G     +++ YE  K WG+KYF  NF RLV+VKT VDPSDFF  EQSIP F+
Sbjct: 484 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of MC03g_new0293 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.9e-151
Identity = 267/534 (50.00%), Postives = 373/534 (69.85%), Query Frame = 0

Query: 10  AFAFACSIVLSSSLF--WAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFSYSSILNF 69
           +F F C I+     F    + A+  E FL+C +++ P+N +  K +YT  N  Y S+LN 
Sbjct: 7   SFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNS 66

Query: 70  SIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVSHLP 129
           +I NLRF++  TPKPL+++ PSH+SHIQ  ++CSK  GLQIRTRSGGHD EG+SY+S +P
Sbjct: 67  TIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVP 126

Query: 130 FIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVGVGGHLS 189
           F+++DL ++RSI ID  + TAWV++GATLGE+YY + +K++ L+  AG CPTV  GGH  
Sbjct: 127 FVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFG 186

Query: 190 GGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGIVVAWKL 249
           GGGYG L+R YGLAAD++IDAHLV+ +G++L+R+SM +DLFW +RGGG  SFGI+VAWK+
Sbjct: 187 GGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKI 246

Query: 250 RLVPVPTTLTTCTVNRTLD-RDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQQG-GK 309
           RLV VP + T  +V + ++  + VKL+++WQ +A+K + +LLL       +I   QG  K
Sbjct: 247 RLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNK 306

Query: 310 TNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGF----SAGES 369
           T     F + FLG VD LV ++N +FPELG+ K DC + SWI++ +  +G     +   +
Sbjct: 307 TAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFN 366

Query: 370 LETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEF 429
            E LL R    N + KIK DYVK+PI E++F  I E+L  +DI        PYGG M E 
Sbjct: 367 KEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEI 426

Query: 430 SESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNY 489
           SES  P+PHRA  +Y++ YI  WE+Q  +  ++HLNW+R IY++M PYV+K+PR AY+NY
Sbjct: 427 SESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNY 486

Query: 490 RDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 536
           RDLDIG N+     NY +A++WG KYFGKNF+RLV+VKT VDP++FFR+EQSIP
Sbjct: 487 RDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 537

BLAST of MC03g_new0293 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 7.4e-151
Identity = 260/534 (48.69%), Postives = 369/534 (69.10%), Query Frame = 0

Query: 10  AFAFACSIVLSSSLF--WAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFSYSSILNF 69
           +F F C I+     F    + A+  E FL+C + + P+N +  KFIYT  +  Y S+LN 
Sbjct: 7   SFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNS 66

Query: 70  SIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVSHLP 129
           +IQNLRF++  TPKPL+++ PS++SHIQA+++CSK  GLQIRTRSGGHD EGLSY+S +P
Sbjct: 67  TIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVP 126

Query: 130 FIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVGVGGHLS 189
           F ++DL ++ ++ +D  + TAWV++GATLGE+YY I + ++  +FP G CPTVGVGGH S
Sbjct: 127 FAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFS 186

Query: 190 GGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGIVVAWKL 249
           GGGYG L+R YGLAAD++IDAHLV+ +G++L+R+SM +DLFW IRGGGG +FGI+ AWK+
Sbjct: 187 GGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKI 246

Query: 250 RLVPVPTTLTTCTVNRTLDRDG-VKLIHRWQYVAHKLEDNLLLGISLTGGHILTQQG-GK 309
           +LV VP+  T  +V + ++  G VKL ++WQ +A+K + +L+L       +I    G  K
Sbjct: 247 KLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNK 306

Query: 310 TNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGF----SAGES 369
           T     F + FLG VD LV ++N +FPELG+ K DC E SWI++T+  +G     +A   
Sbjct: 307 TTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFK 366

Query: 370 LETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEF 429
            E LL R      +  IK DYVK+ I E     I E+L  +++ +   +  PYGG M E 
Sbjct: 367 KEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEI 426

Query: 430 SESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNY 489
           SES  P+PHRA  MY++ Y   WE+Q  +  ++H+NWVR +Y++  PYV+++PR AY+NY
Sbjct: 427 SESAIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNY 486

Query: 490 RDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 536
           RDLD+G+ N     NY +A++WG KYFGKNFNRLV+VKT+ DP++FFR+EQSIP
Sbjct: 487 RDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIP 538

BLAST of MC03g_new0293 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.3e-150
Identity = 261/533 (48.97%), Postives = 369/533 (69.23%), Query Frame = 0

Query: 11  FAFACSIVLSSSLF--WAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFSYSSILNFS 70
           F + C I+     F    + A+  E FL+C +++ P+N + +K +YT  +  Y SILN +
Sbjct: 8   FWYVCKIIFFFLSFNIQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNST 67

Query: 71  IQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVSHLPF 130
           IQNLRF++  TPKPL++I P ++SHIQ  ++CSK  GLQIRTRSGGHD EG+SY+S +PF
Sbjct: 68  IQNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPF 127

Query: 131 IVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVGVGGHLSG 190
           +++DL ++ S+ ID  + TAWV++GATLGE+YY I + ++ L+FPAG CPTVG GGH SG
Sbjct: 128 VIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSG 187

Query: 191 GGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGIVVAWKLR 250
           GGYG L+R YGLAAD++IDAHLV+ +G++L+R+SM +DLFW IRGGGG +FGI+ AWK+R
Sbjct: 188 GGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIR 247

Query: 251 LVPVPTTLTTCTVNRTLD-RDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQQG-GKT 310
           LV VP+  T  +V + ++  + VKL+++WQ +A+  E  LLL       +I   QG  KT
Sbjct: 248 LVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKT 307

Query: 311 NPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGF----SAGESL 370
              + F + F G VD LV ++N +FPELG+ K DC + SWI++ +  +G     +     
Sbjct: 308 TIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKK 367

Query: 371 ETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEFS 430
           E LL R      +  IK DYVK+PI E     I E+L  +D+ +   +F PYGG M E S
Sbjct: 368 EILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEIS 427

Query: 431 ESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNYR 490
           ES  P+PHRA  MY++ YI  WE+Q  +  ++H+NW+R +Y++  PYV+++PR AY+NYR
Sbjct: 428 ESAIPFPHRAGIMYEIWYIASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYR 487

Query: 491 DLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 536
           DLD+G+ N     NY +A++WG KYFGKNFNRLV+VKT+VDP +FFR+EQSIP
Sbjct: 488 DLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of MC03g_new0293 vs. NCBI nr
Match: XP_022152433.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 1108 bits (2867), Expect = 0.0
Identity = 544/544 (100.00%), Postives = 544/544 (100.00%), Query Frame = 0

Query: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60
           MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60

Query: 61  YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL 120
           YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV 180
           SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV
Sbjct: 121 SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV 180

Query: 181 GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG 240
           GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG
Sbjct: 181 GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG 240

Query: 241 IVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILT 300
           IVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILT
Sbjct: 241 IVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILT 300

Query: 301 QQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGE 360
           QQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGE
Sbjct: 301 QQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGE 360

Query: 361 SLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSE 420
           SLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSE
Sbjct: 361 SLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSE 420

Query: 421 FSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVN 480
           FSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVN
Sbjct: 421 FSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVN 480

Query: 481 YRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFSSE 540
           YRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFSSE
Sbjct: 481 YRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFSSE 540

Query: 541 YSVW 544
           YSVW
Sbjct: 541 YSVW 544

BLAST of MC03g_new0293 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 794 bits (2050), Expect = 6.82e-285
Identity = 378/537 (70.39%), Postives = 449/537 (83.61%), Query Frame = 0

Query: 3   SFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSP-SNNSISKFIYTPANFSY 62
           S PL PL+F+     + SSSL+ AAS  KHE FL CL  HSP SN SISK +YTP N SY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  SSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLS 122
           SS+L+FSI+NLRFSTA+TPKPL +I P H+SH+QAAV+CS+THGLQIRTRSGGHDFEGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVG 182
            VSHLPFI++DLI+LRSITIDA N+TAWVQSGATLGELYY IA+KS+TLAFPAG+CPTVG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGI 242
           +GGH SGGGYG ++RK+GLAAD+VIDA+LVDANG+  +RE M +DLFW IRGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQ 302
           VVAWK++LVPVP T+T+C  NRTL+   +KLIHRWQYVA+KL++NL LGI L GG+   Q
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 QGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGES 362
            G KTNP ASF + FLGK DE  AI+NTTFPELGL KE+C+E+SWIES L++AGF  G+S
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDS 363

Query: 363 LETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEF 422
           LE LL R PL N S KIKSDYVKEPISEA  +GIWERL ++D+   Q++FVPYGGRMS+ 
Sbjct: 364 LEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQI 423

Query: 423 SESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNY 482
           SESE P+ HRA N+YK+GYIV W++Q+   EKRHL W+RE+Y YM P+V+KSPR+AYVNY
Sbjct: 424 SESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNY 483

Query: 483 RDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 538
           RDLDIG NN+Y KT+++RA VWG KYFG NFNRLV VKT+VDP DFFRHEQSIPT S
Sbjct: 484 RDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTIS 540

BLAST of MC03g_new0293 vs. NCBI nr
Match: XP_038895208.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 779 bits (2011), Expect = 6.05e-279
Identity = 384/546 (70.33%), Postives = 453/546 (82.97%), Query Frame = 0

Query: 1   MKSFPLNPLAFAFACSIVLSSSLFWAA-SAHKHEGFLQCLTRHSPSNNSISKFIYTPANF 60
           MKSF L P A  F   IVLS S  W   S  KHE F+QCL  +S S   IS  IYTP N 
Sbjct: 1   MKSFLLTPFALTF---IVLSISPSWVVFSTKKHENFVQCLLNNSLSTYPISNLIYTPINS 60

Query: 61  SYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEG 120
           SYSSILNFSIQNLRFS+ ETPKPL +I PSH+SHIQAA+ICSK+H LQIRTRSGGHDFEG
Sbjct: 61  SYSSILNFSIQNLRFSSKETPKPLAIITPSHVSHIQAAIICSKSHALQIRTRSGGHDFEG 120

Query: 121 LSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPT 180
           LSY+S +PF+V+DLI+L+SI ID +N TAWVQSGATLGELYYRIA+KS+TLAFPAGSCPT
Sbjct: 121 LSYISDVPFVVVDLINLKSIAIDVENETAWVQSGATLGELYYRIAEKSRTLAFPAGSCPT 180

Query: 181 VGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSF 240
           VGVGGHLSGGG+G+L+RKYGLAAD+VIDA  +DANG++ +RESM +DLFW IRGGGGGSF
Sbjct: 181 VGVGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKLRLVPVPTTLTTCTVNRTLDR-DGVKLIHRWQYVAHKLEDNLLLGISLTGGHI 300
           GIVVAWK++LV VP T+T C +NR L+  D +KLIHRWQYV +KL++NLLLGISL GG+ 
Sbjct: 241 GIVVAWKVKLVRVPATVTICGINRNLEEEDTIKLIHRWQYVTNKLDENLLLGISLIGGNS 300

Query: 301 LTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSA 360
            TQ+GGKTNP+A F ++FLGKV+ELV I+NT F ELGL KEDC E SWI+S LI AGF  
Sbjct: 301 -TQEGGKTNPMALFSSFFLGKVNELVTILNTNFSELGLAKEDCSEMSWIKSVLIMAGFPK 360

Query: 361 GESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRM 420
            E+LE LL R P   +STKIKSDYVKEPIS+A FK +++RL  +DIE+AQ+MF+PYGGRM
Sbjct: 361 QEALEVLLNRAPPSGLSTKIKSDYVKEPISKASFKTMFKRLKAEDIEVAQIMFIPYGGRM 420

Query: 421 SEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAY 480
           SE SES TP+PHRA N+YK+GY VKW+EQS  AEKRHLNWVR IYDYM P+V+KSPRAAY
Sbjct: 421 SEISESLTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWVRVIYDYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 540
            NYRDLDIG NN+  KT+Y RA+VWGLKYFGKNF+RLV VKT++DP++FFR+EQSIPT +
Sbjct: 481 NNYRDLDIGTNNKCGKTSYNRARVWGLKYFGKNFDRLVHVKTKIDPTNFFRNEQSIPTLT 540

Query: 541 S-EYSV 543
             +YS 
Sbjct: 541 DIKYST 542

BLAST of MC03g_new0293 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 778 bits (2009), Expect = 1.05e-278
Identity = 380/535 (71.03%), Postives = 449/535 (83.93%), Query Frame = 0

Query: 5   PLNPLAFAFACSIVLSSSLFWAAS-AHKHEGFLQCLTRHSP--SNNSISKFIYTPANFSY 64
           PL  L+ AF   I LSSS  WA++  +KHE FLQCL  HS   SN SISK I+TP N SY
Sbjct: 6   PLISLSLAF---IFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSY 65

Query: 65  SSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLS 124
           SS+L+FSI+NLRFST ETPKPLL++ PSH+SHIQAAVICSK++G QIRTRSGGHDFEGLS
Sbjct: 66  SSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLS 125

Query: 125 YVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVG 184
           YV+HLPFI++DLI+LRSI+ID K++TAWVQSGATLGELYY IAQKS+TLAFPAG CPTVG
Sbjct: 126 YVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVG 185

Query: 185 VGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGI 244
           VGGH SGGGYG L+RKYGLAAD+VIDA+LVDA+G+  NRESM +DLFW IRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGI 245

Query: 245 VVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQ 304
           VVAWK++LVPVP T+T C+ NRTL+   VK+ HRWQYVA+KL++NL LGI   GG I +Q
Sbjct: 246 VVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQ 305

Query: 305 QGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIEST-LITAGFSAGE 364
           +GGKTNPVA+F + FLGK DEL++I+NT FPEL L K+DCIETSWIEST L+  GF    
Sbjct: 306 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTEL 365

Query: 365 SLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSE 424
           +LE LL R PL N S+KIKSDYVKEP+SEA+ +GIW+RL++QDIE   ++FVPYGGRMS+
Sbjct: 366 TLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQ 425

Query: 425 FSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVN 484
            SESETP+ HRA  +YK+GYI  WEEQ   AEKRHL W+RE+YDYM P+V+KSPRAAY N
Sbjct: 426 ISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYAN 485

Query: 485 YRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 535
           YRDLDIG NN++ KT+Y++A VWGLKYFG NFNRLV VKT+VDP DFFRHEQSIP
Sbjct: 486 YRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 537

BLAST of MC03g_new0293 vs. NCBI nr
Match: XP_022152518.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 768 bits (1984), Expect = 6.21e-275
Identity = 372/538 (69.14%), Postives = 450/538 (83.64%), Query Frame = 0

Query: 1   MKSFPLNPLAFAFACSIVLSSSLFWAA-SAHKHEGFLQCLTRHSPSNNSISKFIYTPANF 60
           MK  PL  L+ AF   I+LSSS  WAA SA KHE FLQCL+ HS  ++SISK IYTP N 
Sbjct: 1   MKYSPLTILSLAF---ILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINP 60

Query: 61  SYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEG 120
           SYSS+LNFS++NLRFS+AETPKPLL+I PS++SH+QAAVICSKTHGLQIR RSGGHD EG
Sbjct: 61  SYSSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEG 120

Query: 121 LSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPT 180
           LSYV+HLPFI++DLI+L+SIT++ +NSTAW+QSGATLGELYY IAQKS+TLAFPAG CPT
Sbjct: 121 LSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPT 180

Query: 181 VGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSF 240
           VGVGGH SGGGYG ++RKYGLAAD+VIDA+L+DANG+  +RESM +DLFW IRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHIL 300
           GIVVAWK++LVPVP  +TTC+ +R LD D + LIHRWQ VA+KL++NL  GI L GG+  
Sbjct: 241 GIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNAS 300

Query: 301 TQQG-GKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSA 360
           T+ G  K NP A+F + FLGK DEL+AI+NT FPEL L K+DC+ETSWIESTL+ A  + 
Sbjct: 301 TEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQ 360

Query: 361 G-ESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGR 420
             ESLE+LL R PL   +TK+KSDYVKEPI +A  +GIWERL  QDIE+ Q++FVPYGGR
Sbjct: 361 TIESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGR 420

Query: 421 MSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAA 480
           MS  S+SETP+ HRA N+YK+GYIV W+EQS  AE+RHL+W+REIYDYM P+V+KSPRA 
Sbjct: 421 MSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRAT 480

Query: 481 YVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 535
           YVNYRDLDIG NN+Y KT+Y++A VWG KYFG NFNRLV +K++VDPS+FF++EQSIP
Sbjct: 481 YVNYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIP 535

BLAST of MC03g_new0293 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 1108 bits (2867), Expect = 0.0
Identity = 544/544 (100.00%), Postives = 544/544 (100.00%), Query Frame = 0

Query: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60
           MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60

Query: 61  YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL 120
           YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL
Sbjct: 61  YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV 180
           SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV
Sbjct: 121 SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV 180

Query: 181 GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG 240
           GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG
Sbjct: 181 GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG 240

Query: 241 IVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILT 300
           IVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILT
Sbjct: 241 IVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILT 300

Query: 301 QQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGE 360
           QQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGE
Sbjct: 301 QQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGE 360

Query: 361 SLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSE 420
           SLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSE
Sbjct: 361 SLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSE 420

Query: 421 FSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVN 480
           FSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVN
Sbjct: 421 FSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVN 480

Query: 481 YRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFSSE 540
           YRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFSSE
Sbjct: 481 YRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFSSE 540

Query: 541 YSVW 544
           YSVW
Sbjct: 541 YSVW 544

BLAST of MC03g_new0293 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 794 bits (2050), Expect = 3.30e-285
Identity = 378/537 (70.39%), Postives = 449/537 (83.61%), Query Frame = 0

Query: 3   SFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSP-SNNSISKFIYTPANFSY 62
           S PL PL+F+     + SSSL+ AAS  KHE FL CL  HSP SN SISK +YTP N SY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  SSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLS 122
           SS+L+FSI+NLRFSTA+TPKPL +I P H+SH+QAAV+CS+THGLQIRTRSGGHDFEGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVG 182
            VSHLPFI++DLI+LRSITIDA N+TAWVQSGATLGELYY IA+KS+TLAFPAG+CPTVG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGI 242
           +GGH SGGGYG ++RK+GLAAD+VIDA+LVDANG+  +RE M +DLFW IRGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQ 302
           VVAWK++LVPVP T+T+C  NRTL+   +KLIHRWQYVA+KL++NL LGI L GG+   Q
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 QGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGES 362
            G KTNP ASF + FLGK DE  AI+NTTFPELGL KE+C+E+SWIES L++AGF  G+S
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSAGFKIGDS 363

Query: 363 LETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEF 422
           LE LL R PL N S KIKSDYVKEPISEA  +GIWERL ++D+   Q++FVPYGGRMS+ 
Sbjct: 364 LEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQI 423

Query: 423 SESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNY 482
           SESE P+ HRA N+YK+GYIV W++Q+   EKRHL W+RE+Y YM P+V+KSPR+AYVNY
Sbjct: 424 SESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNY 483

Query: 483 RDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 538
           RDLDIG NN+Y KT+++RA VWG KYFG NFNRLV VKT+VDP DFFRHEQSIPT S
Sbjct: 484 RDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTIS 540

BLAST of MC03g_new0293 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 778 bits (2009), Expect = 5.10e-279
Identity = 380/535 (71.03%), Postives = 449/535 (83.93%), Query Frame = 0

Query: 5   PLNPLAFAFACSIVLSSSLFWAAS-AHKHEGFLQCLTRHSP--SNNSISKFIYTPANFSY 64
           PL  L+ AF   I LSSS  WA++  +KHE FLQCL  HS   SN SISK I+TP N SY
Sbjct: 6   PLISLSLAF---IFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSY 65

Query: 65  SSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLS 124
           SS+L+FSI+NLRFST ETPKPLL++ PSH+SHIQAAVICSK++G QIRTRSGGHDFEGLS
Sbjct: 66  SSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLS 125

Query: 125 YVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVG 184
           YV+HLPFI++DLI+LRSI+ID K++TAWVQSGATLGELYY IAQKS+TLAFPAG CPTVG
Sbjct: 126 YVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVG 185

Query: 185 VGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGI 244
           VGGH SGGGYG L+RKYGLAAD+VIDA+LVDA+G+  NRESM +DLFW IRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGI 245

Query: 245 VVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQ 304
           VVAWK++LVPVP T+T C+ NRTL+   VK+ HRWQYVA+KL++NL LGI   GG I +Q
Sbjct: 246 VVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQ 305

Query: 305 QGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIEST-LITAGFSAGE 364
           +GGKTNPVA+F + FLGK DEL++I+NT FPEL L K+DCIETSWIEST L+  GF    
Sbjct: 306 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTEL 365

Query: 365 SLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSE 424
           +LE LL R PL N S+KIKSDYVKEP+SEA+ +GIW+RL++QDIE   ++FVPYGGRMS+
Sbjct: 366 TLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQ 425

Query: 425 FSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVN 484
            SESETP+ HRA  +YK+GYI  WEEQ   AEKRHL W+RE+YDYM P+V+KSPRAAY N
Sbjct: 426 ISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYAN 485

Query: 485 YRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 535
           YRDLDIG NN++ KT+Y++A VWGLKYFG NFNRLV VKT+VDP DFFRHEQSIP
Sbjct: 486 YRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 537

BLAST of MC03g_new0293 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 768 bits (1984), Expect = 3.01e-275
Identity = 372/538 (69.14%), Postives = 450/538 (83.64%), Query Frame = 0

Query: 1   MKSFPLNPLAFAFACSIVLSSSLFWAA-SAHKHEGFLQCLTRHSPSNNSISKFIYTPANF 60
           MK  PL  L+ AF   I+LSSS  WAA SA KHE FLQCL+ HS  ++SISK IYTP N 
Sbjct: 1   MKYSPLTILSLAF---ILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINP 60

Query: 61  SYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEG 120
           SYSS+LNFS++NLRFS+AETPKPLL+I PS++SH+QAAVICSKTHGLQIR RSGGHD EG
Sbjct: 61  SYSSVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEG 120

Query: 121 LSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPT 180
           LSYV+HLPFI++DLI+L+SIT++ +NSTAW+QSGATLGELYY IAQKS+TLAFPAG CPT
Sbjct: 121 LSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPT 180

Query: 181 VGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSF 240
           VGVGGH SGGGYG ++RKYGLAAD+VIDA+L+DANG+  +RESM +DLFW IRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHIL 300
           GIVVAWK++LVPVP  +TTC+ +R LD D + LIHRWQ VA+KL++NL  GI L GG+  
Sbjct: 241 GIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNAS 300

Query: 301 TQQG-GKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSA 360
           T+ G  K NP A+F + FLGK DEL+AI+NT FPEL L K+DC+ETSWIESTL+ A  + 
Sbjct: 301 TEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQ 360

Query: 361 G-ESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGR 420
             ESLE+LL R PL   +TK+KSDYVKEPI +A  +GIWERL  QDIE+ Q++FVPYGGR
Sbjct: 361 TIESLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGR 420

Query: 421 MSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAA 480
           MS  S+SETP+ HRA N+YK+GYIV W+EQS  AE+RHL+W+REIYDYM P+V+KSPRA 
Sbjct: 421 MSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRAT 480

Query: 481 YVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 535
           YVNYRDLDIG NN+Y KT+Y++A VWG KYFG NFNRLV +K++VDPS+FF++EQSIP
Sbjct: 481 YVNYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIP 535

BLAST of MC03g_new0293 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 767 bits (1981), Expect = 2.40e-274
Identity = 378/537 (70.39%), Postives = 436/537 (81.19%), Query Frame = 0

Query: 3   SFPLNPLAFAFACSIVLSSSLFWA-ASAHKHEGFLQCLTRHSPSNN-SISKFIYTPANFS 62
           S PL PL  AF   I LSSS FW  A A KHE FLQCL  HSP  N SISK I+TP N S
Sbjct: 6   SSPLIPLFLAF---IFLSSSRFWTLAVADKHESFLQCLLNHSPDGNYSISKVIHTPINSS 65

Query: 63  YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL 122
           YSS+L+FSI+NLRFSTAETPKPLL+I PSH+SHIQAAV+CSK +G QIRTRSGGHDFE L
Sbjct: 66  YSSVLDFSIRNLRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERL 125

Query: 123 SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV 182
           SYV+HL FI++DLI+LRSI++D KN+ AWV SGATLGELYY IAQKS+T AFPAG CPTV
Sbjct: 126 SYVAHLSFIIVDLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTV 185

Query: 183 GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG 242
           GVGGH SGGGYG L+RKYGLAAD+VIDA+LVDANG+  +RESM +DLFW IRGGGGGSFG
Sbjct: 186 GVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFG 245

Query: 243 IVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILT 302
           IVVAWK++LVPVP T+T C  NRTL+   VKL HRWQYVA+KL++NL LGI   GG++ +
Sbjct: 246 IVVAWKVKLVPVPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLGGNVTS 305

Query: 303 QQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITA-GFSAG 362
           Q GGKTNPVA+F + FLG+ DEL+AI+NT FPEL L K+DCIETSWIEST++   GF   
Sbjct: 306 QGGGKTNPVATFFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMGIGFQTK 365

Query: 363 ESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMS 422
            +LE LL R PL NISTKIKSDYVKEPISEA  +GI ERLN QDIE   ++FVPYGGRMS
Sbjct: 366 VTLEALLSRTPLTNISTKIKSDYVKEPISEATIQGIGERLNAQDIESGNLIFVPYGGRMS 425

Query: 423 EFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYV 482
           +  ESETP+ HRA  +YK+GYI  W +QS   EKRHL+W+RE+Y YMAP+V+KSPRA Y 
Sbjct: 426 QIFESETPFSHRAGYLYKIGYITSWLDQSIDTEKRHLSWIRELYSYMAPFVSKSPRAVYA 485

Query: 483 NYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPT 536
           NYRDLDIG N  Y KT+Y++A  WG KYFG NFNRLV VKT+VDP DFFRHEQSIPT
Sbjct: 486 NYRDLDIGSNKRYGKTSYKQASTWGFKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 539

BLAST of MC03g_new0293 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 539.7 bits (1389), Expect = 2.8e-153
Identity = 265/537 (49.35%), Postives = 365/537 (67.97%), Query Frame = 0

Query: 2   KSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFSY 61
           +SF  + L F    +++LS     +A+      FLQCL+     +N +SK I+TP + S+
Sbjct: 4   QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSF 63

Query: 62  SSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLS 121
           SS+L  SIQN RFS  + PKP+L++ P   S +Q+AV C++  G+ IRTRSGGHD+EGLS
Sbjct: 64  SSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLS 123

Query: 122 YVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVG 181
           YV+H PF++LDL +LRSIT+D  N + WVQ+GAT+GELYY I +K++TLAFPAG CPTVG
Sbjct: 124 YVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVG 183

Query: 182 VGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGI 241
           VGGH SGGGYG L+RK+GLAADHVIDA +VDA GRIL R  M +D FW IRGGGG SF +
Sbjct: 184 VGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCV 243

Query: 242 VVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQ 301
           V++WK+ L+ VP+T+T   V +  ++  +K+IHRWQ+VA K+ D+L + + L        
Sbjct: 244 VLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------- 303

Query: 302 QGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGES 361
           Q  K    ASFP  +LG V  L+ +VN  FPELGL ++DC E SWIES +  A    GE 
Sbjct: 304 QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA--ELGEE 363

Query: 362 LETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEF 421
              +L +R   +++ K KSD+V+EP+ +     +W RL   + E AQ++F P+GG+MSE 
Sbjct: 364 PINVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 423

Query: 422 SESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNY 481
           ++ ETP+PHR  N+Y++ Y+  W       +++++ WV  +YD M+ +V KSPR AY+N 
Sbjct: 424 ADYETPFPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINL 483

Query: 482 RDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 539
           RDLD+G     +++ YE  K WG+KYF  NF RLV+VKT VDPSDFF  EQSIP F+
Sbjct: 484 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of MC03g_new0293 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 522.7 bits (1345), Expect = 3.5e-148
Identity = 257/521 (49.33%), Postives = 356/521 (68.33%), Query Frame = 0

Query: 19  LSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFSYSSILNFSIQNLRFSTAE 78
           L+ S   +A    HE FL+CL+     N    K I+T  + S+ SIL+ SIQN RFS +E
Sbjct: 15  LNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSE 74

Query: 79  TPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVSH-LPFIVLDLIHLR 138
           TPKP+ +I P   S +Q  + C++ HG+ +RTRS GH +EGLSY+++  PF V+DL +LR
Sbjct: 75  TPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLR 134

Query: 139 SITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVGVGGHLSGGGYGYLIRK 198
           SI++D  N T WVQ+GAT GELYY I + +++LAFPAG  PTVGVGG  SGGGYG L+RK
Sbjct: 135 SISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRK 194

Query: 199 YGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGIVVAWKLRLVPVPTTLT 258
           YGLAAD++IDA +VDA+GRIL+R++M +D FW IRGGGG SFG++++WK++LV VP+T+T
Sbjct: 195 YGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTIT 254

Query: 259 TCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQQGGKTNPVASFPTYFL 318
              V +T  ++ V++I +WQY A K+ D+L +  +L       ++  K    A F   ++
Sbjct: 255 VFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTL-------ERSNKNAVHALFTGLYI 314

Query: 319 GKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGESLETLLYRRPLVNISTK 378
           G V+ L+A++   FPELGL KE C E SWIES L  A F  GESL  +L  R   ++S K
Sbjct: 315 GPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESL-GVLTNRERTSLSFK 374

Query: 379 IKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEFSESETPYPHRAENMYK 438
            K D+V+EPI EA  + IW RL   +  L +++  P+GG+MSE +E ETP+PHR  N+Y+
Sbjct: 375 GKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYE 434

Query: 439 MGYIVKWEEQSAKAE---KRHLNWVREIYDYMAPYVTKSPRAAYVNYRDLDIGRNNEYEK 498
           + Y+  W E+  K +    ++L WV  +Y++M PYV+KSPR AYVN++D+D+G     +K
Sbjct: 435 IQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKK 494

Query: 499 TNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 536
           T YE  K WG+KYF  NF RLV+VKTRVDP+DFF  EQSIP
Sbjct: 495 TKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of MC03g_new0293 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 513.8 bits (1322), Expect = 1.6e-145
Identity = 259/532 (48.68%), Postives = 361/532 (67.86%), Query Frame = 0

Query: 11  FAFACSIVLSSSLFWAASAHK--HEGFLQCLTRHSPSNNSISKFIYTPANFSYSSILNFS 70
           F +   + LS S F + SA +  HE FL+CL+     N   S+ I+T  + SY SILN S
Sbjct: 11  FIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHR--INEDDSRIIHTSKDPSYFSILNSS 70

Query: 71  IQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVS-HLP 130
           IQN RF   ETPKP+ +I P   + +Q+ + C++ HG+ IRTRSGGHD+EGLSY++   P
Sbjct: 71  IQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRP 130

Query: 131 FIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVGVGGHLS 190
           F+V+DL +LRSIT+D  N T WVQSGAT+GELYY I + S++LAFPAG  PTVG+GG   
Sbjct: 131 FVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFG 190

Query: 191 GGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGIVVAWKL 250
           GGGYG L+RKYGL+AD+VIDAH+VDANG  L+R+ M +D FW IRGGGG SF +V++WK+
Sbjct: 191 GGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKI 250

Query: 251 RLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQQGGKTN 310
           RL+ VP+ +T   V +T +++ V +I++WQY+A K+ ++L +   L           +T 
Sbjct: 251 RLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ---------KETE 310

Query: 311 PVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGESLETLLY 370
             ASFP  +LG V +L+A++   FPELGL   +C E SWIES L    F  GES+E +L 
Sbjct: 311 VYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESME-ILA 370

Query: 371 RRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEFSESETP 430
           +R   + S K K D+++EPI +   + +W R    +  LA+++  P+GG+MSE +++E P
Sbjct: 371 KRKRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIP 430

Query: 431 YPHRAENMYKMGYIVKWEEQSAKAE---KRHLNWVREIYDYMAPYVTKSPRAAYVNYRDL 490
           +PHR  N+Y++ Y+  W E+  K +   +++L WV  +Y++M PYV+KSPR AYVN+RD+
Sbjct: 431 FPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDI 490

Query: 491 DIGRNNEYE-KTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 536
           D+G       KT YE AKVWG+KYF  NF+RLV+VKT VDP DFF  EQSIP
Sbjct: 491 DLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of MC03g_new0293 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 504.6 bits (1298), Expect = 9.9e-143
Identity = 255/531 (48.02%), Postives = 360/531 (67.80%), Query Frame = 0

Query: 13  FACSIVLSSSLF-W--AASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFSYSSILNFSI 72
           +A  +VL   +F W  ++S+   E F QCLT +S   + IS  I+   N SYSS+L  +I
Sbjct: 3   YALILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANI 62

Query: 73  QNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVSH--LP 132
           +NLRF+T  TPKP L+IA +H SH+QAA+ C K H LQ++ RSGGHD++GLSYV++   P
Sbjct: 63  RNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKP 122

Query: 133 FIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVGVGGHLS 192
           F VLD+ +LRS+ +D  + TAWVQ+GA LGE+YY I +KS+TLA+PAG CPTVGVGGH+S
Sbjct: 123 FFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHIS 182

Query: 193 GGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGIVVAWKL 252
           GGGYG ++RKYGL  D+ IDA +VD NG+IL+R+ M +DL+W I GGGGGS+G+V+A+K+
Sbjct: 183 GGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKI 242

Query: 253 RLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLG--ISLTGGHILTQQGGK 312
            LV VP  +T   ++RTL+++   +IHRWQ VA KL D L +   I +  G + +Q+  +
Sbjct: 243 NLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVR 302

Query: 313 TNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGESLETL 372
           T    +F   FLG    L++I+N  FPELGL + DC ETSWI+S L       G S   L
Sbjct: 303 T----TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLL 362

Query: 373 LYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEFSESE 432
           L R   VN   K KSDYV+EPIS    + IW+++   ++E+  + F PYGG M   S + 
Sbjct: 363 LQRNQPVNY-LKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPYGGEMGRISSTV 422

Query: 433 TPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNYRDLD 492
           TP+P+RA N++K+ Y   W +++     R++   R++Y +M P+V+K+PR ++ NYRD+D
Sbjct: 423 TPFPYRAGNLWKIQYGANWRDET--LTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVD 482

Query: 493 IGRNNEYEK-TNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 536
           +G N+   K ++Y   K +G KYF  NF RLV++KTRVD  +FFR+EQSIP
Sbjct: 483 LGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of MC03g_new0293 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 502.7 bits (1293), Expect = 3.8e-142
Identity = 246/513 (47.95%), Postives = 349/513 (68.03%), Query Frame = 0

Query: 29  AHKHEGFLQCLTRHSPSNNSISKFIYTPA-NFS-YSSILNFSIQNLRFSTAETPKPLLVI 88
           A   + F+ C+ R++     + K  + P  N S +S +L  + QNLRF     PKP  + 
Sbjct: 29  ASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLRFLKKSMPKPGFIF 88

Query: 89  APSHISHIQAAVICSKTHGLQIRTRSGGHDFEGLSYVSHL--PFIVLDLIHLRSITIDAK 148
           +P H SH+QA++ICSK   + +R RSGGHD+EGLSYVS +  PFI++DL  +R + I+ +
Sbjct: 89  SPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQ 148

Query: 149 NSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTVGVGGHLSGGGYGYLIRKYGLAADH 208
           +++AWVQSGAT+GELYYRIA+KS+   FPAG C ++G+GGH++GG YG ++RKYGL AD+
Sbjct: 149 DNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADN 208

Query: 209 VIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFGIVVAWKLRLVPVPTTLTTCTVNRT 268
           V+DA +VDANG++L+R +M +D FW IRGG GGSFGI++AWK++LVPVP T+T  TV +T
Sbjct: 209 VLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKTVTVFTVTKT 268

Query: 269 LDRD-GVKLIHRWQYVAHKLEDNLLLGISLTGGHILTQQGGKTNPVASFPTYFLGKVDEL 328
           L +D G K+I +WQ VA KL + L + +       +   GG      S+   FLG    L
Sbjct: 269 LQQDVGNKIISKWQRVADKLVEELFIRVLFN----VAGTGGNKTVTTSYNALFLGGKGTL 328

Query: 329 VAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAGESLETLLY-RRPLVNISTKIKSDY 388
           + ++  +FPELGLT +DCIE SW+ES    +GF        LL  + P   +S K KSD+
Sbjct: 329 MNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPKVSFKAKSDF 388

Query: 389 VKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMSEFSESETPYPHRAENMYKMGYIV 448
           VK PI E+  +GI+++L  +DI L  +++ PYGG M++  ES+ P+PHR   ++K+ Y+ 
Sbjct: 389 VKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKGVLFKVQYVT 448

Query: 449 KWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYVNYRDLDIGRNNEYEKTNYERAKV 508
            W + S K   RH+NW+R++Y YM PYV+ +PR AYVNYRDLD+GRN +  KT  ++A+V
Sbjct: 449 SWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQV 508

Query: 509 WGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 536
           WG  YF  NFNRL+ +K +VDP +FFRHEQSIP
Sbjct: 509 WGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534