MC03g1275 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g1275
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDiacylglycerol kinase
LocationMC03: 18642635 .. 18649304 (+)
RNA-Seq ExpressionMC03g1275
SyntenyMC03g1275
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGGAGAAGATTCTTTTATGGAATCTGTATTTCTCAAATTTGGAAACAAAATATTAAAGATTCTAAAATTTATTTGATTTGAAAAATTAAAGAAAAAAAGAAGAGAGCGAGAGAGAGGAAAAAAAAAAAAAAGAAAAGAAAGGAGAGAGAGCGAGACTTCCATCTGAAATTCTCAAATCCGTACCTTTCCCCCGACATCGAAAGCTTCGGATTTGGGTTTTGCTACGATTCCTTTGATTGAAGGAATAGCGCGTTTATACTTTTTGAAGATCGACTTAGGGTTTTCTGTGATCGGGGCGTTTGATCCTCCCTCTTATTCACTTGTTGTTTACAGTAAGTTTTATTTGTCATGAGGTTCTTGTGTGTCCATTTTCAGCGCTCTATGCGTGTAGTTTTTTAAATGAATTGGTGTGTTCTTTCTCTTCTTCCCTCGTTTTGGTATTGAGAGGAAGCTAAGAACACGTTTAGTAAGGTTTCCGGGCAGTTTGGTATGAACTTTAATATTTTTTGCTGCTTCTTTTTTTCTGCTACGATTCTTAGCTGCTAAGCGCCACATTGGGATTGCTGGTCTGGTGGTTTTCGAACTTAGATTCTTCTTTTATCCAAATACTGTTAACCTCTGTTGAGTTCTTCTTTTGCTTATTATTCACGTTTCGATTAATTGACTTAGCCTTTGTAATTACTTTCCTTCTTGACAATAAACATACTGTTGAGACCTTCTTCTCGTTAATCCTAAAATTTGTAATTTTTGGTATATTCCCTTCAATATCTTCTTCTAGAATTTGATATGACCTTGAACTCAAAATATTCTTAGGCTTACATTCGAATTTCATTGTCTGATGATAGCTCTAAACGTGCTTGTTTTTCTATCTTTTTAATTACAGCGTTCACGCCGGCAAGGCTTTTTTAAAACATTTTATTGTTTCACTTTTTTCAGGAAATATGCGCTTGCCTGTCAATATTTTATTTTGACTCCCGGAATTGGTCGGGTTGACATCCGTGATACTGAGATGTGAAAGCATTTTATTTCGGGGTACCACATTTAAGTTAACAATGTCATTACCGAGTCGCTTAAGTTGAGTAAAGTGTTGAAATTAGCTTCATCGTTTAAATTGGATTACTTCGGAGTTTGGGTGGTAACTGGCCGTTTGTCTCTGTCTGGAGCCAAATTAGTTGTTTCAGTCATTAGATGATTCTCTTCTCATATTCGAGAATAAGATATGGACGAGGACAGTGAAATTGGATTGCTGTTCCCTAGTTGGAACAGCAAAAATCCAACTGATCGTCTTTTTATTATTTCTTGCTTTATTGCTGCTATCATTGGAATTTTGACAATAGCCTTTACGGCCTTTCAGTGGCGGAGAAATATCAATCTAGGTTGGATGAAAGCTATAGCCAGGTCGAAGAGAAACCCCAAAAAGACTAATAAAGTTCCTGTAGCTGCTCACGACTGGATTCTAGAATCTGTATCTCGTGGAAAGAATTTGAATTGTTGTGTGTGCTTAAGGTTTATGTCCCCTTCACAAACTCTCGGTCCTATGGTCGCTTCAGACAGTTTTATTCACTGTTGCAGCATTTGTGGTGCTGCAGCTCACCTGAGTTGTTCTTCTGGTGCTCAAAAGGATTGCAAGTGTGTATCTATGATTGGCTTTGAGCATGTGATGCACCAATGGGCTGTTCGGTGGACTGAGATTACTGACCAACCTGATGAAACTTCTTTCTGTAGCTACTGCGAAGAACCATGTAGTGGGTCTTTTCTAGGGGGTTCTCCAATATGGTGTTGCTTATGGTGCCAACGTCTAGTGCATGTGGATTGCCATAGTAGTATGTGTAATGAAACAGGTGATATTTGTGATTTAGGTTCATTTAGAAGGTTGATTCTCTCACCGCTTTATGTGAAGGAGTCAATTAGGACGTCCTCAGGTGGATTTTTGAGCTCCATCACCCATGGAGCAAATGAGATTGCATCATCAGTGCGTGCTAGTATAAGGAGTAAGGGTAAGAAGCACAAACATGGTCGTAAACCATCTATTGATACAGGAACAACTGGTAGTACTTGTGATATGTCTACTGAAAGCACAGCTGATGCTCATCACAGAGTTAATGGTTATCATGGAACTGAGAGAAACTGTAATGGTAGTGCAGCTTCAGAGGTTCAACATCAAAATGGTGATATGGATGGTAAAAATAGTTCAAATCAAAACTTCAAAAGAAGTTCTGCAGTCAATCAGAAGGATGAATCTCATATATTAGGATTGAAACTTAGATATGAGGTAATTGAACTGCCTCCTGATTCACGTCCCTTATTAGTATTTATCAACAAGAAGAGTGGAGCCCGCCGTGGTGATTCTCTCAAACAACGGTTAAACATGCTTTTGAATCCTGCCCAGGTATGATTTTTTCTCCACTCTATTCAATTTTGGCTTTATCTTTAGAAGTAGACGGTTGTATGCTATAGCGCAAATTAATTTTCTCAGCATGCTCTGTACTCTTAGTTGTTGGATACTTCATGATTGATGAGGCAGCCAACAATGTCTTGTGTATAGTTTGTTCACAACTTCCATTTCCTTTTGGAATTTGGTGGAAGTTGGAGCATGTATGTCTGGGTCAATCGAAAAGACTATTGAGGTCAATGAAATCCTCTTAAGTGACTGATATATTTTTTTGTGCTATAAGAAATGTTTTTATGAGAAAGGAAAAGTTCCAAATGTGTAGGGTTAATTCTCCAACCTTTCCAAAATTTTGGAGGATTGTTTTCACTGCATGTTAAATATTTTACAAGGTTAATGGACTGTATGCTAACATTTCAGGTTTTTGAGTTGAGCTCTGCCCAAGGTCCTGAATCTGGTCTTTATTTATTCAGAAAAGTGCCACACTTTAAGGTTCTTGTATGTGGGGGCGATGGAACTGTTGGTTGGGTCCTAAACTGTATAGACAAGCAAAATTTTGTTTCTCCTCCTCCAGTTGCTATACTTCCTGCTGGAACAGGAAATGATCTGGCTAGAGTATTAAACTGGGGTGGTGGTTTGGGCTCAGTGGAGAGACAGGGGGGTCTTTGCACAGTCTTGCATCATGTAGAAAATGCTGCAGTGACTGTTCTTGACCGTTGGAAGGTGGCGATAGTGGATCAACAAGGGAAGCAACTCAAATCCCCACAATTTATGAATAATTATCTCGGTATGTGAATAGGTTTGATAGTGTTTCTGTATTTAAGATATTGGTATTAACTTTGACCGACTTCAGGGCACATTTGAACTGCATGTCTTCTCTTCAGGGTTCTTGTATGGAGCCCTCGTAGCATCTTTCTTCTTCTCTAATTTCTCTCTTTGCTTGTTTTTTACTCATTATTACCAATTTGTCCCCCAAATGCAGGAATTGGATGTGATGCTAAGGTTGCTCTTGATATTCATAATCTGCGGGAAGAAAATCCAGAAAAATTTTATAACCAGGTGATGTAAATTGTAGAATTAAAGGTAACCATAACTTGAAAACAATCCATGACATTAAGTCGTTCTGACCATAACTGCAATCATGCATGGCTGCTACTACAAGTAGAACTTCTCATATCGTATGGTTTTATTGTTTTGAGAAAGGAAAGGCAGCTTGCTATTTTGTGTAGTGATATCTTGCATTATTAAACGTGGGTCATTTTATGACCATTTATTTCCTTTGTTTGATGGCTATTTTTCAAACTCTTTGTTGCCAAAAAACAATGGTATAATGGACGGTGAACATCTTACGGAACCTGAGAGAATTTACTACTGTATGGTAGAGATCTCGATGGGATACTTGCTTCTATTAGTAAGTCGTCGGCATAATATCGTAGTTCAGAAATTTGTCTCCCTGACTGCACTTGCATAAGTATCTAACAAATTTACATGTATGACCTAACACAAACTTGATTTTACTTCTATAGTGTATATGTAACTTATGCATTGAATAAATTGACCACTTCTATTAGAGATAATCTATTCTCAATACTTTTCTTTCTGTTGTACTCTGCTTTGGCTTTTCCTTTTATAAATGATGTCTATTATTTTATTTTACCTTCTTCTAAGTGATGCCCATAAATAGATATCCTGAGAGAGCATGCTTGCAGTTGACTTGCACATGCTTGCATGCTTTCTCAATGAGGACAAGTCAATCTTGTGCATTTTGCAAAACCACAGGCATCTCTTATGCATTGAACTTCTATTTTTTAGGTTTTATATTTATCCAAATTTGCAGAAATTATTGCTAGCTGTTTCTTCTTTGGTGTCTCTGATTTAGGCGACTTGTTGAAATTGTTTATTATTGCTACTACAATGTTTCCTCCTATTCCAGATGACTAAGTTCATTTGGGTAGTTCATATCACTTATCTGACACTATTACGTTACTACAGTTCATGAATAAAGTTCTTTATGCAAGAGAAGGAGCCAAGAGCATAATGGACAGGACATTTGCAGATATTCCATGGCAAGTTCGGGTGGAAGTGGATGGTGTGGAGGTAGAGGTTCCTGAGGTGAGAACTTAACTTTGAATTATTTAATTCTGAAAATATAACTAAATTGTCAATACGTCACTATATTTTTTTTATTATTGAGAAGTTTTAGTATGATGTTCAATATCATGATTATACATTTTAGTTTACAAGTGTGAATTCTTCCAACCGTCCTGAATTATTTACTTGAGGTCGATTAATTAAACCTTTAATTTGAACATTTCAGGATGCAGAAGGTGTACTTGTTGCGAACATTGGAAGCTACATGGGTGGTGTGGATCTATGGCATAATGAGGATGAAACTTTTGATAATTTTGATCCACAGTCCATGCATGATAAAGTACTCGAGGTTGTCAGCATATCGGGAACCTGGCACCTTGGGAAGCTACAGGTATACATTGATTCAAACTAATTTTCCTGTCCCCATGTGCTATCATGCCCATTTGCCGAAGATACCAAATTTGATGGATCTATGTAGGATGAATCTACTTCTAGTCTTCACGTGTAATGATGCGTTTTCATGTCTTTTTCTGCTCTGCTCTCATAATAATGGGTTGACCTTAGGACTTTAGTGGGTCTAAGGGTGGAGTTTGTTGTATGCATATTTCAGTTATTCTATTTTCGAGCATGAAGCTGAAAGTAAGTAGTTATGTAATTTCTTAGCTTCAATCAGGTTTTATAAAGATGTTATTTTCCAGCAAAATGGATGGTAAAGTCATGCAAGGCTGTCTTAGTATCAGGTCATGTATCAATCCTGGATCGTATGTTAGAGGTCATCAGAGCGATAATTACGTGAAGTGGTTGGAATTGGGCAAGGGACGAGGTTCATACGTAGAGTATCGTTGCACCCCCCTGGGTTGGATGTTTCATAGTTTCTACTTTCTTTTTGAAACAGGTGGGGCTTTCTCGAGCTAGAAGACTTGCACAAGGCCAGTCGATTAAGATACAATTGTGTGCTTCGTTGCCTGTTCAAATTGATGGCGAGCCCTGGTTTCAGAATCAGTGTACTCTAGTTATATCCCATCATGGACAGGTCTTACTCTTGTTCTCTCCTTCTTTCATCTCCCTCGTGGGGCTAGTTACTTGTGCAATATCATGCATAGTCTCCCTCTCCTCTTCCTGGGAAAAAAAACAAAATCATGCATGATATTTCTCTACTGTTAATGCAAGTAAGGCATATAAATGACGTTTTATGAGCCTTGGCTAAGTCCATATTATATTTTTGAACAACAAACCACTTTTCATTGATAGACGAAAAGATGCAAATTGTTTCTCTTGAGGGATAAAAGAGAAAAAAATCAACAGCCAACCAAAGAACGTGGCCTAAAACAGCAACACATTAAGAAATGAACCAAAAACAAGCATAAAAAAAAGCCATAACCATAACACCAGCTAGCTAAGGCCATATTATAGAATCTGGTGGTGCAGCCTAATCAGTTTCAAACATTTGATTCCAAAGAATTGGCTTTGGTGAATCTAATAGTTCCTTGGATCTGTTTGTGTTGGTGATCTGTTTATGTTGTGAGCAGAGGCTTGTTCTCAAACATCCACTTTTACGTATAGTTTAGCACTCTGTTTCTACATTCACTTGTTATGATCTGACACTTTTCCGTTAATTTGCTTTAAAGTACCTATCTTCAAGCTCTGTAATGGTTTGAATTTATATTGGAAAAAAACCTTTGTAGGCCTTCATGTTGAAAAGAGCAGTTGAGGAGCCTCTCGGTCATGCAGCTGCAATTGTCACTGATGTTCTGGAGAGTGCTGAATCTAATAATGTAATAAATGCTACACAAAAGCGAGCGCTCCTCCAAGAAATGGCAATCAGATTAACTTGATAGTTTCTTTAGTCATTCCTCCTGTTATGATCGGGGTTTTGAGTACGAATCGTTTTCAGCTTATTATACCACCCCTCGCATCTCTCCATATAGGTAAGATCTTGTGGTGCTTTTTGTTTTCTTTTTCTTCTTGTTTCCCTTGATGGGTAATATCTGAGTCATGATTGTATATCAACGATATCTATTATATAAATATACACATATACATAGAGTTCCTCAACATAGATCAGTTTCAAGGTTTTCTTTGTTTTTTTTTTCTCGAAATACTTTCTGGTGTACGTATATGGTCGAGATTGAACAATATCATGCAGTTGTGATCTTGTTCATACTCAACCACATACTCAGTAAAAAAT

mRNA sequence

CAAGGAGAAGATTCTTTTATGGAATCTGTATTTCTCAAATTTGGAAACAAAATATTAAAGATTCTAAAATTTATTTGATTTGAAAAATTAAAGAAAAAAAGAAGAGAGCGAGAGAGAGGAAAAAAAAAAAAAAGAAAAGAAAGGAGAGAGAGCGAGACTTCCATCTGAAATTCTCAAATCCGTACCTTTCCCCCGACATCGAAAGCTTCGGATTTGGGTTTTGCTACGATTCCTTTGATTGAAGGAATAGCGCGTTTATACTTTTTGAAGATCGACTTAGGGTTTTCTGTGATCGGGGCGTTTGATCCTCCCTCTTATTCACTTGTTGTTTACAGAAATATGCGCTTGCCTGTCAATATTTTATTTTGACTCCCGGAATTGGTCGGGTTGACATCCGTGATACTGAGATGTGAAAGCATTTTATTTCGGGGTACCACATTTAAGTTAACAATGTCATTACCGAGTCGCTTAAGTTGAGTAAAGTGTTGAAATTAGCTTCATCGTTTAAATTGGATTACTTCGGAGTTTGGGTGGTAACTGGCCGTTTGTCTCTGTCTGGAGCCAAATTAGTTGTTTCAGTCATTAGATGATTCTCTTCTCATATTCGAGAATAAGATATGGACGAGGACAGTGAAATTGGATTGCTGTTCCCTAGTTGGAACAGCAAAAATCCAACTGATCGTCTTTTTATTATTTCTTGCTTTATTGCTGCTATCATTGGAATTTTGACAATAGCCTTTACGGCCTTTCAGTGGCGGAGAAATATCAATCTAGGTTGGATGAAAGCTATAGCCAGGTCGAAGAGAAACCCCAAAAAGACTAATAAAGTTCCTGTAGCTGCTCACGACTGGATTCTAGAATCTGTATCTCGTGGAAAGAATTTGAATTGTTGTGTGTGCTTAAGGTTTATGTCCCCTTCACAAACTCTCGGTCCTATGGTCGCTTCAGACAGTTTTATTCACTGTTGCAGCATTTGTGGTGCTGCAGCTCACCTGAGTTGTTCTTCTGGTGCTCAAAAGGATTGCAAGTGTGTATCTATGATTGGCTTTGAGCATGTGATGCACCAATGGGCTGTTCGGTGGACTGAGATTACTGACCAACCTGATGAAACTTCTTTCTGTAGCTACTGCGAAGAACCATGTAGTGGGTCTTTTCTAGGGGGTTCTCCAATATGGTGTTGCTTATGGTGCCAACGTCTAGTGCATGTGGATTGCCATAGTAGTATGTGTAATGAAACAGGTGATATTTGTGATTTAGGTTCATTTAGAAGGTTGATTCTCTCACCGCTTTATGTGAAGGAGTCAATTAGGACGTCCTCAGGTGGATTTTTGAGCTCCATCACCCATGGAGCAAATGAGATTGCATCATCAGTGCGTGCTAGTATAAGGAGTAAGGGTAAGAAGCACAAACATGGTCGTAAACCATCTATTGATACAGGAACAACTGGTAGTACTTGTGATATGTCTACTGAAAGCACAGCTGATGCTCATCACAGAGTTAATGGTTATCATGGAACTGAGAGAAACTGTAATGGTAGTGCAGCTTCAGAGGTTCAACATCAAAATGGTGATATGGATGGTAAAAATAGTTCAAATCAAAACTTCAAAAGAAGTTCTGCAGTCAATCAGAAGGATGAATCTCATATATTAGGATTGAAACTTAGATATGAGGTAATTGAACTGCCTCCTGATTCACGTCCCTTATTAGTATTTATCAACAAGAAGAGTGGAGCCCGCCGTGGTGATTCTCTCAAACAACGGTTAAACATGCTTTTGAATCCTGCCCAGGTTTTTGAGTTGAGCTCTGCCCAAGGTCCTGAATCTGGTCTTTATTTATTCAGAAAAGTGCCACACTTTAAGGTTCTTGTATGTGGGGGCGATGGAACTGTTGGTTGGGTCCTAAACTGTATAGACAAGCAAAATTTTGTTTCTCCTCCTCCAGTTGCTATACTTCCTGCTGGAACAGGAAATGATCTGGCTAGAGTATTAAACTGGGGTGGTGGTTTGGGCTCAGTGGAGAGACAGGGGGGTCTTTGCACAGTCTTGCATCATGTAGAAAATGCTGCAGTGACTGTTCTTGACCGTTGGAAGGTGGCGATAGTGGATCAACAAGGGAAGCAACTCAAATCCCCACAATTTATGAATAATTATCTCGGAATTGGATGTGATGCTAAGGTTGCTCTTGATATTCATAATCTGCGGGAAGAAAATCCAGAAAAATTTTATAACCAGTTCATGAATAAAGTTCTTTATGCAAGAGAAGGAGCCAAGAGCATAATGGACAGGACATTTGCAGATATTCCATGGCAAGTTCGGGTGGAAGTGGATGGTGTGGAGGTAGAGGTTCCTGAGGATGCAGAAGGTGTACTTGTTGCGAACATTGGAAGCTACATGGGTGGTGTGGATCTATGGCATAATGAGGATGAAACTTTTGATAATTTTGATCCACAGTCCATGCATGATAAAGTACTCGAGGTTGTCAGCATATCGGGAACCTGGCACCTTGGGAAGCTACAGGTGGGGCTTTCTCGAGCTAGAAGACTTGCACAAGGCCAGTCGATTAAGATACAATTGTGTGCTTCGTTGCCTGTTCAAATTGATGGCGAGCCCTGGTTTCAGAATCAGTGTACTCTAGTTATATCCCATCATGGACAGGCCTTCATGTTGAAAAGAGCAGTTGAGGAGCCTCTCGGTCATGCAGCTGCAATTGTCACTGATGTTCTGGAGAGTGCTGAATCTAATAATGTAATAAATGCTACACAAAAGCGAGCGCTCCTCCAAGAAATGGCAATCAGATTAACTTGATAGTTTCTTTAGTCATTCCTCCTGTTATGATCGGGGTTTTGAGTACGAATCGTTTTCAGCTTATTATACCACCCCTCGCATCTCTCCATATAGGTAAGATCTTGTGGTGCTTTTTGTTTTCTTTTTCTTCTTGTTTCCCTTGATGGGTAATATCTGAGTCATGATTGTATATCAACGATATCTATTATATAAATATACACATATACATAGAGTTCCTCAACATAGATCAGTTTCAAGGTTTTCTTTGTTTTTTTTTTCTCGAAATACTTTCTGGTGTACGTATATGGTCGAGATTGAACAATATCATGCAGTTGTGATCTTGTTCATACTCAACCACATACTCAGTAAAAAAT

Coding sequence (CDS)

ATGGACGAGGACAGTGAAATTGGATTGCTGTTCCCTAGTTGGAACAGCAAAAATCCAACTGATCGTCTTTTTATTATTTCTTGCTTTATTGCTGCTATCATTGGAATTTTGACAATAGCCTTTACGGCCTTTCAGTGGCGGAGAAATATCAATCTAGGTTGGATGAAAGCTATAGCCAGGTCGAAGAGAAACCCCAAAAAGACTAATAAAGTTCCTGTAGCTGCTCACGACTGGATTCTAGAATCTGTATCTCGTGGAAAGAATTTGAATTGTTGTGTGTGCTTAAGGTTTATGTCCCCTTCACAAACTCTCGGTCCTATGGTCGCTTCAGACAGTTTTATTCACTGTTGCAGCATTTGTGGTGCTGCAGCTCACCTGAGTTGTTCTTCTGGTGCTCAAAAGGATTGCAAGTGTGTATCTATGATTGGCTTTGAGCATGTGATGCACCAATGGGCTGTTCGGTGGACTGAGATTACTGACCAACCTGATGAAACTTCTTTCTGTAGCTACTGCGAAGAACCATGTAGTGGGTCTTTTCTAGGGGGTTCTCCAATATGGTGTTGCTTATGGTGCCAACGTCTAGTGCATGTGGATTGCCATAGTAGTATGTGTAATGAAACAGGTGATATTTGTGATTTAGGTTCATTTAGAAGGTTGATTCTCTCACCGCTTTATGTGAAGGAGTCAATTAGGACGTCCTCAGGTGGATTTTTGAGCTCCATCACCCATGGAGCAAATGAGATTGCATCATCAGTGCGTGCTAGTATAAGGAGTAAGGGTAAGAAGCACAAACATGGTCGTAAACCATCTATTGATACAGGAACAACTGGTAGTACTTGTGATATGTCTACTGAAAGCACAGCTGATGCTCATCACAGAGTTAATGGTTATCATGGAACTGAGAGAAACTGTAATGGTAGTGCAGCTTCAGAGGTTCAACATCAAAATGGTGATATGGATGGTAAAAATAGTTCAAATCAAAACTTCAAAAGAAGTTCTGCAGTCAATCAGAAGGATGAATCTCATATATTAGGATTGAAACTTAGATATGAGGTAATTGAACTGCCTCCTGATTCACGTCCCTTATTAGTATTTATCAACAAGAAGAGTGGAGCCCGCCGTGGTGATTCTCTCAAACAACGGTTAAACATGCTTTTGAATCCTGCCCAGGTTTTTGAGTTGAGCTCTGCCCAAGGTCCTGAATCTGGTCTTTATTTATTCAGAAAAGTGCCACACTTTAAGGTTCTTGTATGTGGGGGCGATGGAACTGTTGGTTGGGTCCTAAACTGTATAGACAAGCAAAATTTTGTTTCTCCTCCTCCAGTTGCTATACTTCCTGCTGGAACAGGAAATGATCTGGCTAGAGTATTAAACTGGGGTGGTGGTTTGGGCTCAGTGGAGAGACAGGGGGGTCTTTGCACAGTCTTGCATCATGTAGAAAATGCTGCAGTGACTGTTCTTGACCGTTGGAAGGTGGCGATAGTGGATCAACAAGGGAAGCAACTCAAATCCCCACAATTTATGAATAATTATCTCGGAATTGGATGTGATGCTAAGGTTGCTCTTGATATTCATAATCTGCGGGAAGAAAATCCAGAAAAATTTTATAACCAGTTCATGAATAAAGTTCTTTATGCAAGAGAAGGAGCCAAGAGCATAATGGACAGGACATTTGCAGATATTCCATGGCAAGTTCGGGTGGAAGTGGATGGTGTGGAGGTAGAGGTTCCTGAGGATGCAGAAGGTGTACTTGTTGCGAACATTGGAAGCTACATGGGTGGTGTGGATCTATGGCATAATGAGGATGAAACTTTTGATAATTTTGATCCACAGTCCATGCATGATAAAGTACTCGAGGTTGTCAGCATATCGGGAACCTGGCACCTTGGGAAGCTACAGGTGGGGCTTTCTCGAGCTAGAAGACTTGCACAAGGCCAGTCGATTAAGATACAATTGTGTGCTTCGTTGCCTGTTCAAATTGATGGCGAGCCCTGGTTTCAGAATCAGTGTACTCTAGTTATATCCCATCATGGACAGGCCTTCATGTTGAAAAGAGCAGTTGAGGAGCCTCTCGGTCATGCAGCTGCAATTGTCACTGATGTTCTGGAGAGTGCTGAATCTAATAATGTAATAAATGCTACACAAAAGCGAGCGCTCCTCCAAGAAATGGCAATCAGATTAACTTGA

Protein sequence

MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALLQEMAIRLT
Homology
BLAST of MC03g1275 vs. ExPASy Swiss-Prot
Match: Q39017 (Diacylglycerol kinase 1 OS=Arabidopsis thaliana OX=3702 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 538/732 (73.50%), Postives = 618/732 (84.43%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTD----RLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMK 60
           MD+D E+G+ FPSW SKNP D    R  + SCF+AA++GILTIA+TAFQWRRNINL W K
Sbjct: 1   MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTK 60

Query: 61  AIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHC 120
           AIARSK+NPK  +KVPVA H W L+ ++R KNLNCCVCL+ MSPSQ +   VAS+SF H 
Sbjct: 61  AIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAI---VASESFFHR 120

Query: 121 CSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCS 180
           C+ICGAAAH +CSS A KDCKCVSM+GFEHV+HQWAVRWTE  DQ D++SFCSYC+E CS
Sbjct: 121 CTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCS 180

Query: 181 GSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGG 240
            SFLGGSPIWCCLWCQRLVHVDCHS+M NETGDICDLG  RRLIL PLYVKE  R  SGG
Sbjct: 181 SSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGG 240

Query: 241 FLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNG 300
           FLSSITHGANE+AS+  ASIR + KK+K   + S DTG +GS CD STESTAD    VNG
Sbjct: 241 FLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNG 300

Query: 301 YHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELP 360
            H    N       +  + + D +GK     + KR+ +  QK E H L  KL+YE+ +LP
Sbjct: 301 AHAVLENSISVMNGDSSNGDSDSNGKLEKKPSVKRTGSFGQK-EYHALRSKLKYELADLP 360

Query: 361 PDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVL 420
            D+RPLLVFINKKSGA+RGDSL+QRL++ LNP QVFELSS QGPE GL+LFRKVPHF+VL
Sbjct: 361 SDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHFRVL 420

Query: 421 VCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL 480
           VCGGDGT GWVL+ I+KQNF+SPP VAILPAGTGNDL+RVLNWGGGLGSVERQGGL TVL
Sbjct: 421 VCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVL 480

Query: 481 HHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFY 540
            ++E+AAVTVLDRWKV+I++QQGKQL+ P++MNNY+G+GCDAKVAL+IHNLREENPE+FY
Sbjct: 481 QNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPERFY 540

Query: 541 NQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVD 600
           +QFMNKVLYAREGA+SIMDRTF D PWQVRVEVDGV++EVPEDAEG+LVANIGSYMGGVD
Sbjct: 541 SQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVD 600

Query: 601 LWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASL 660
           LW NEDET++NFDPQSMHDK++EVVSISGTWHLGKLQVGLSRARRLAQG ++KIQLCA L
Sbjct: 601 LWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPL 660

Query: 661 PVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQK 720
           PVQIDGEPW Q  CTL ISHHGQAFMLKRA EEPLGHAAAI+TDVLE+AE+N VINA+QK
Sbjct: 661 PVQIDGEPWNQQPCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQK 720

Query: 721 RALLQEMAIRLT 729
           R LLQEMA+RLT
Sbjct: 721 RTLLQEMALRLT 728

BLAST of MC03g1275 vs. ExPASy Swiss-Prot
Match: Q9FFN7 (Diacylglycerol kinase 2 OS=Arabidopsis thaliana OX=3702 GN=DGK2 PE=1 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 1.6e-201
Identity = 354/726 (48.76%), Postives = 476/726 (65.56%), Query Frame = 0

Query: 6   EIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIARSKRNP 65
           E+G     W   +  D  FI    +   +G+L + +T  +W++  +L W+KA AR K+  
Sbjct: 3   EVGFSLIQWLISSGADSPFIFGWLVTGSVGLLAVIYTFLKWQKKTSLNWVKAAAREKKKV 62

Query: 66  KKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSICGAAAH 125
            K  +VP++ H W  +     +   CCVCL  + P Q +    +    +H C++CG AAH
Sbjct: 63  WKRLRVPLSHHQWTDDYGYGQQPSTCCVCLYSLVPGQNVSNKASLSIPVHRCAVCGVAAH 122

Query: 126 LSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFLGGSPI 185
             CSS A KDCKCV+  G +HV H W+ RW  + D  D T+FC YC+EPC   F+  SP+
Sbjct: 123 FYCSSSAAKDCKCVAQAGSDHVRHHWSERWVNMDDNADMTAFCFYCDEPCGIPFIEASPM 182

Query: 186 WCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSSITHGA 245
           W CLWCQRL+HV CH  M  E+GD CDLGS RR+ILSP++VK +      G L++I    
Sbjct: 183 WHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRVILSPVHVKLNEANGVDGVLTTI---K 242

Query: 246 NEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGTERNCN 305
           NE+A S+R  +R K  + K+G   S++    G   + S          VNG     +   
Sbjct: 243 NELA-SIRGHVRRKRHRGKNGNGQSLN----GKLLEDSVSDPVKT--VVNGL--VVKKLR 302

Query: 306 GSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSRPLLVF 365
              + +   Q  DM     + +  +     +++++S  L    ++ +++LPPD+RPLLVF
Sbjct: 303 RDRSIDCLKQVSDM----PNAKGLQNGIGGHKRNKSAALNFMKKFSLVDLPPDARPLLVF 362

Query: 366 INKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVG 425
           IN KSG + G  L +RLNMLLNP QVFEL S QGP++GL L  KV +F+VLVCGGDGTV 
Sbjct: 363 INAKSGGQLGPFLHRRLNMLLNPVQVFELGSCQGPDAGLDLCSKVKYFRVLVCGGDGTVA 422

Query: 426 WVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVT 485
           WVL+ I+K+NF SPPPVAILP GTGNDL+RVL WG G+  V+ QG L T L  +++AAVT
Sbjct: 423 WVLDAIEKRNFESPPPVAILPLGTGNDLSRVLQWGRGISVVDGQGSLRTFLQDIDHAAVT 482

Query: 486 VLDRWKVAIVDQQGKQL---KSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNK 545
           +LDRW V IV++  ++    +  +FM NYLGIGCDAKVA + H +R+E PEKF +QF+NK
Sbjct: 483 MLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNK 542

Query: 546 VLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNED 605
           + YA+EGA+ IMDR  AD+PWQV +EVDG ++E+P+D+EG++V NIGSYMGGVDLW N+ 
Sbjct: 543 LRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDY 602

Query: 606 ETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQIDG 665
           E  DNF  Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQG+ I+I + +  PVQIDG
Sbjct: 603 EHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQIDG 662

Query: 666 EPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALLQE 725
           EP+ Q    L I+HHGQ FML+RA +EP GHAAAI+ +VL  AE   VINA+QK+ LLQ+
Sbjct: 663 EPFIQQPGCLEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKVLLQQ 712

Query: 726 MAIRLT 729
           MA+ L+
Sbjct: 723 MALHLS 712

BLAST of MC03g1275 vs. ExPASy Swiss-Prot
Match: P49621 (Diacylglycerol kinase beta OS=Rattus norvegicus OX=10116 GN=Dgkb PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 4.1e-72
Identity = 146/353 (41.36%), Postives = 196/353 (55.52%), Query Frame = 0

Query: 357 PDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVL 416
           P + PLLVF+N KSG ++G+ + ++   LLNP QV+ L S  GP  GL+ FR VP F+VL
Sbjct: 431 PGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSL-SGNGPMPGLHFFRDVPDFRVL 490

Query: 417 VCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL 476
            CGGDGTVGW+L+CI+K N V  PPVAILP GTGNDLAR L WGGG         L  +L
Sbjct: 491 ACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGY----EGENLMKIL 550

Query: 477 HHVENAAVTVLDRWKVAIVDQQGKQLKSP---QFMNNYLGIGCDAKVALDIHNLREENPE 536
             +E++   +LDRWK  +      +   P     +NNY  IG DA +A   H +RE++PE
Sbjct: 551 KDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPE 610

Query: 537 KFYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVE-VPEDAEGVLVANIGSYM 596
           KF ++  NK  Y   G       T   +   V +E DGV+++ +    +G+ + NI S  
Sbjct: 611 KFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLQGIAILNIPSMH 670

Query: 597 GGVDLWHNE---------------------DETFDNFDPQSMHDKVLEVVSISGTWHLGK 656
           GG +LW                        D     F  Q + D++LEVV + G   +G+
Sbjct: 671 GGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQ 730

Query: 657 LQVGL-SRARRLAQGQSIKIQLCASLPVQIDGEPWFQNQCTLVISHHGQAFML 684
           +  GL S  RRLAQ  S+ I+   SLP+QIDGEPW Q  CT+ I+H  QA ML
Sbjct: 731 IYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPML 778

BLAST of MC03g1275 vs. ExPASy Swiss-Prot
Match: Q91WG7 (Diacylglycerol kinase gamma OS=Mus musculus OX=10090 GN=Dgkg PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 5.4e-72
Identity = 157/385 (40.78%), Postives = 215/385 (55.84%), Query Frame = 0

Query: 323 NSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSRPLLVFINKKSGARRGDSLKQRL 382
           +   Q  K   +V  K E     L  +Y++I   P + PLLV +N KSG R+G+ + ++ 
Sbjct: 399 SGDRQGGKSDGSVAAKGE-----LVTQYKIIP-SPGTHPLLVLVNPKSGGRQGERILRKF 458

Query: 383 NMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPV 442
           + LLNP QVF L +  GP  GL  F   P F+VL CGGDGTVGW+L+CIDK NF   PPV
Sbjct: 459 HYLLNPEQVFNLDNG-GPTPGLNFFHDTPDFRVLACGGDGTVGWILDCIDKANFTKHPPV 518

Query: 443 AILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQQ---- 502
           A+LP GTGNDLAR L WGGG       G L  +L  +E + + +LDRW + ++ ++    
Sbjct: 519 AVLPLGTGNDLARCLRWGGGY----EGGSLTKILKEIEQSPLVMLDRWYLEVMPREEVEN 578

Query: 503 GKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTF 562
           G Q+     MNNY  IG DA +A   H +RE++PEKF ++  NK+ Y   G       T 
Sbjct: 579 GDQVPY-NIMNNYFSIGVDASIAHRFHMMREKHPEKFNSRMKNKLWYFEFGTSETFAATC 638

Query: 563 ADIPWQVRVEVDGVEVEVPE-DAEGVLVANIGSYMGGVDLWHNEDETFDN---------- 622
             +   + +E DGVEV++     EG+ + NI S  GG +LW    ET  N          
Sbjct: 639 KKLHDHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLW---GETKKNRAVIRESRKS 698

Query: 623 -FDP-------QSMHDKVLEVVSISGTWHLGKLQVGL-SRARRLAQGQSIKIQLCASLPV 682
             DP       Q + D++LEVV + G   +G++  GL S  RRLAQ  S+ I+    LP+
Sbjct: 699 VTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTNKLLPM 758

Query: 683 QIDGEPWFQNQCTLVISHHGQAFML 684
           Q+DGEPW Q QCT+ I+H  QA M+
Sbjct: 759 QVDGEPWMQPQCTIKITHKNQAPMM 768

BLAST of MC03g1275 vs. ExPASy Swiss-Prot
Match: Q6NS52 (Diacylglycerol kinase beta OS=Mus musculus OX=10090 GN=Dgkb PE=1 SV=2)

HSP 1 Score: 273.5 bits (698), Expect = 7.0e-72
Identity = 154/403 (38.21%), Postives = 217/403 (53.85%), Query Frame = 0

Query: 307 SAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSRPLLVFI 366
           + AS  + +      + SS+Q   +++  N+   ++ + +  +   I   P + PLLVF+
Sbjct: 382 AGASVPEERQSTAKKEKSSSQQPNKATDKNKMQRANSVTMDGQGLQITPVPGTHPLLVFV 441

Query: 367 NKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGW 426
           N KSG ++G+ + ++   LLNP QV+ L S  GP  GL+ FR VP F+VL CGGDGTVGW
Sbjct: 442 NPKSGGKQGERIYRKFQYLLNPRQVYSL-SGNGPMPGLHFFRDVPDFRVLACGGDGTVGW 501

Query: 427 VLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTV 486
           +L+CI+K N V  PPVAILP GTGNDLAR L WGGG         L  +L  +E++   +
Sbjct: 502 ILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGY----EGENLMKILKDIESSTEIM 561

Query: 487 LDRWKVAIVDQQGKQLKSP---QFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKV 546
           LDRWK  +      +   P     +NNY  IG DA +A   H +RE++PEKF ++  NK 
Sbjct: 562 LDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKF 621

Query: 547 LYAREGAKSIMDRTFADIPWQVRVEVDGVEVE-VPEDAEGVLVANIGSYMGGVDLWHNE- 606
            Y   G       T   +   V +E DGV+++ +    EG+ + NI S  GG +LW    
Sbjct: 622 WYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESK 681

Query: 607 --------------------DETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGL-SRAR 666
                               D     F  Q + D++LEVV + G   +G++  GL S  R
Sbjct: 682 KKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGR 741

Query: 667 RLAQGQSIKIQLCASLPVQIDGEPWFQNQCTLVISHHGQAFML 684
           RLAQ  S+ I+   SLP+QIDGEPW Q  CT+ I+H  QA ML
Sbjct: 742 RLAQCSSVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPML 779

BLAST of MC03g1275 vs. NCBI nr
Match: XP_022137252.1 (diacylglycerol kinase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1487 bits (3849), Expect = 0.0
Identity = 728/728 (100.00%), Postives = 728/728 (100.00%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60
           MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR
Sbjct: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120

Query: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300
           ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT
Sbjct: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300

Query: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360
           ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR
Sbjct: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480

Query: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660
           EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI
Sbjct: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660

Query: 661 DGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALL 720
           DGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALL
Sbjct: 661 DGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALL 720

Query: 721 QEMAIRLT 728
           QEMAIRLT
Sbjct: 721 QEMAIRLT 728

BLAST of MC03g1275 vs. NCBI nr
Match: XP_022137253.1 (diacylglycerol kinase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 1377 bits (3563), Expect = 0.0
Identity = 674/674 (100.00%), Postives = 674/674 (100.00%), Query Frame = 0

Query: 55  MKAIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFI 114
           MKAIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFI
Sbjct: 1   MKAIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFI 60

Query: 115 HCCSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEP 174
           HCCSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEP
Sbjct: 61  HCCSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEP 120

Query: 175 CSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSS 234
           CSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSS
Sbjct: 121 CSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSS 180

Query: 235 GGFLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRV 294
           GGFLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRV
Sbjct: 181 GGFLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRV 240

Query: 295 NGYHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIE 354
           NGYHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIE
Sbjct: 241 NGYHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIE 300

Query: 355 LPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFK 414
           LPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFK
Sbjct: 301 LPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFK 360

Query: 415 VLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCT 474
           VLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCT
Sbjct: 361 VLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCT 420

Query: 475 VLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEK 534
           VLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEK
Sbjct: 421 VLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEK 480

Query: 535 FYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGG 594
           FYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGG
Sbjct: 481 FYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGG 540

Query: 595 VDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCA 654
           VDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCA
Sbjct: 541 VDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCA 600

Query: 655 SLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINAT 714
           SLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINAT
Sbjct: 601 SLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINAT 660

Query: 715 QKRALLQEMAIRLT 728
           QKRALLQEMAIRLT
Sbjct: 661 QKRALLQEMAIRLT 674

BLAST of MC03g1275 vs. NCBI nr
Match: XP_022924022.1 (diacylglycerol kinase 1-like [Cucurbita moschata] >XP_022924023.1 diacylglycerol kinase 1-like [Cucurbita moschata] >KAG7020006.1 Diacylglycerol kinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1351 bits (3497), Expect = 0.0
Identity = 661/729 (90.67%), Postives = 695/729 (95.34%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60
           MDE+ EIGLLFPSWNSKNPTDRLF+ISC  AAIIGILTIAFTAFQWRRNINL WMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNL+CCVCL+F+SPSQTLGPMVASDSFIH C+IC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           G AAHLSCSS AQKDCKCVSMIGFE+VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKES RTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300
           ITHGANEIASSVRASIRS+ KK+KH RKPSI    +GS  DMSTESTAD+HH VNGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360
           ERN NGS  SE+QHQNGD+D KN SN +FK++S+ NQKD +H+ G+ LRYEVIELPPDSR
Sbjct: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHV-GMNLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNP QVFELSSAQGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVE 480

Query: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660
           EDETFD+FD QSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SI+IQLCA+LPVQI
Sbjct: 601 EDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQI 660

Query: 661 DGEPWFQN-QCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRAL 720
           DGEPWFQ+  CTLVISHHG+AFMLKRAVEEPLGHAAAI+TDVLESAESNNVINA+QKR L
Sbjct: 661 DGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720

Query: 721 LQEMAIRLT 728
           LQE+A RLT
Sbjct: 721 LQEIAKRLT 728

BLAST of MC03g1275 vs. NCBI nr
Match: XP_023000647.1 (diacylglycerol kinase 1-like [Cucurbita maxima] >XP_023000648.1 diacylglycerol kinase 1-like [Cucurbita maxima])

HSP 1 Score: 1349 bits (3492), Expect = 0.0
Identity = 661/729 (90.67%), Postives = 696/729 (95.47%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60
           MDE+ EIGLLFPSWNSKNPTDRLF+ISC  AAIIGILTIAFTAFQWRRNINL WMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNL+CCVCL+F+SPSQTLGPMVASDSFIH C+IC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           G AAHLSCSS AQKDCKCVSMIGFE+VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLY+KES RTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYIKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300
           ITHGANEIAS+VRASIRS+ KK+KH RKPSI    +GS  DMSTESTAD+HH VNGY+GT
Sbjct: 241 ITHGANEIASTVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360
           ERN NGS  SE+QHQNGD+D KN SN +FK+SS+ NQKDE+H+ G+ LRYEVIELPPDSR
Sbjct: 301 ERNINGSRTSELQHQNGDID-KNISNPSFKKSSSFNQKDETHV-GMNLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNP QVFELSSAQGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVE 480

Query: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660
           EDETFD+FD QSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SI+IQLCA+LPVQI
Sbjct: 601 EDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQI 660

Query: 661 DGEPWFQN-QCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRAL 720
           DGEPWFQ+  CTLVISHHG+AFMLKRAVEEPLGHAAAI+TDVLESAESNNVINA+QKR L
Sbjct: 661 DGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720

Query: 721 LQEMAIRLT 728
           LQE+A RLT
Sbjct: 721 LQEIAKRLT 727

BLAST of MC03g1275 vs. NCBI nr
Match: XP_023519698.1 (diacylglycerol kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1348 bits (3490), Expect = 0.0
Identity = 659/729 (90.40%), Postives = 695/729 (95.34%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60
           MDE+ EIGLLFPSWNSKNPTDRLF+ISC  AAIIGILTIAFTAFQWRRNINL WMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNL+CCVCL+F+SPSQTLGPMVASDSFIH C+IC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           G AAHLSCSS AQKDCKCVSMIGFE+VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKES RTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300
           ITHGANEIASSVRASIRS+ KK+KH R+PSI    +GS  DMSTESTAD+HH VNGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRRPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360
           ERN NGS  SE+QHQNGD++ KN SN +FK++S+ NQKDE+H+ G+ LRYEVIELPPDSR
Sbjct: 301 ERNINGSRTSELQHQNGDINDKNISNPSFKKNSSFNQKDETHV-GMNLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNP QVFELSSAQGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVE 480

Query: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAV VLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVAVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660
           EDETFD+FD QSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SI+IQLCA+LPVQI
Sbjct: 601 EDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQI 660

Query: 661 DGEPWFQN-QCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRAL 720
           DGEPWFQ+  CTLVISHHG+AFMLKRAVEEPLGHAAAI+TDVLESAESNNVINA+QKR L
Sbjct: 661 DGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720

Query: 721 LQEMAIRLT 728
           LQE+A RLT
Sbjct: 721 LQEIAKRLT 728

BLAST of MC03g1275 vs. ExPASy TrEMBL
Match: A0A6J1C7R2 (Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111008758 PE=3 SV=1)

HSP 1 Score: 1487 bits (3849), Expect = 0.0
Identity = 728/728 (100.00%), Postives = 728/728 (100.00%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60
           MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR
Sbjct: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120

Query: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300
           ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT
Sbjct: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300

Query: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360
           ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR
Sbjct: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480

Query: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660
           EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI
Sbjct: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660

Query: 661 DGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALL 720
           DGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALL
Sbjct: 661 DGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALL 720

Query: 721 QEMAIRLT 728
           QEMAIRLT
Sbjct: 721 QEMAIRLT 728

BLAST of MC03g1275 vs. ExPASy TrEMBL
Match: A0A6J1C9S8 (Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111008758 PE=3 SV=1)

HSP 1 Score: 1377 bits (3563), Expect = 0.0
Identity = 674/674 (100.00%), Postives = 674/674 (100.00%), Query Frame = 0

Query: 55  MKAIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFI 114
           MKAIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFI
Sbjct: 1   MKAIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFI 60

Query: 115 HCCSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEP 174
           HCCSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEP
Sbjct: 61  HCCSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEP 120

Query: 175 CSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSS 234
           CSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSS
Sbjct: 121 CSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSS 180

Query: 235 GGFLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRV 294
           GGFLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRV
Sbjct: 181 GGFLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRV 240

Query: 295 NGYHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIE 354
           NGYHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIE
Sbjct: 241 NGYHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIE 300

Query: 355 LPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFK 414
           LPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFK
Sbjct: 301 LPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFK 360

Query: 415 VLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCT 474
           VLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCT
Sbjct: 361 VLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCT 420

Query: 475 VLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEK 534
           VLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEK
Sbjct: 421 VLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEK 480

Query: 535 FYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGG 594
           FYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGG
Sbjct: 481 FYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGG 540

Query: 595 VDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCA 654
           VDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCA
Sbjct: 541 VDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCA 600

Query: 655 SLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINAT 714
           SLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINAT
Sbjct: 601 SLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINAT 660

Query: 715 QKRALLQEMAIRLT 728
           QKRALLQEMAIRLT
Sbjct: 661 QKRALLQEMAIRLT 674

BLAST of MC03g1275 vs. ExPASy TrEMBL
Match: A0A6J1E7Q5 (Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111431571 PE=3 SV=1)

HSP 1 Score: 1351 bits (3497), Expect = 0.0
Identity = 661/729 (90.67%), Postives = 695/729 (95.34%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60
           MDE+ EIGLLFPSWNSKNPTDRLF+ISC  AAIIGILTIAFTAFQWRRNINL WMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNL+CCVCL+F+SPSQTLGPMVASDSFIH C+IC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           G AAHLSCSS AQKDCKCVSMIGFE+VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKES RTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300
           ITHGANEIASSVRASIRS+ KK+KH RKPSI    +GS  DMSTESTAD+HH VNGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360
           ERN NGS  SE+QHQNGD+D KN SN +FK++S+ NQKD +H+ G+ LRYEVIELPPDSR
Sbjct: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHV-GMNLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNP QVFELSSAQGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVE 480

Query: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660
           EDETFD+FD QSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SI+IQLCA+LPVQI
Sbjct: 601 EDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQI 660

Query: 661 DGEPWFQN-QCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRAL 720
           DGEPWFQ+  CTLVISHHG+AFMLKRAVEEPLGHAAAI+TDVLESAESNNVINA+QKR L
Sbjct: 661 DGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720

Query: 721 LQEMAIRLT 728
           LQE+A RLT
Sbjct: 721 LQEIAKRLT 728

BLAST of MC03g1275 vs. ExPASy TrEMBL
Match: A0A6J1KN74 (Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111495021 PE=3 SV=1)

HSP 1 Score: 1349 bits (3492), Expect = 0.0
Identity = 661/729 (90.67%), Postives = 696/729 (95.47%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60
           MDE+ EIGLLFPSWNSKNPTDRLF+ISC  AAIIGILTIAFTAFQWRRNINL WMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNL+CCVCL+F+SPSQTLGPMVASDSFIH C+IC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           G AAHLSCSS AQKDCKCVSMIGFE+VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLY+KES RTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYIKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300
           ITHGANEIAS+VRASIRS+ KK+KH RKPSI    +GS  DMSTESTAD+HH VNGY+GT
Sbjct: 241 ITHGANEIASTVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360
           ERN NGS  SE+QHQNGD+D KN SN +FK+SS+ NQKDE+H+ G+ LRYEVIELPPDSR
Sbjct: 301 ERNINGSRTSELQHQNGDID-KNISNPSFKKSSSFNQKDETHV-GMNLRYEVIELPPDSR 360

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420
           PLLVFINKKSGARRGDSLKQRLNMLLNP QVFELSSAQGPESGLYLFRKVPHFKVLVCGG
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGG 420

Query: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVE 480
           DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HVE
Sbjct: 421 DGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVE 480

Query: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540
           NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM
Sbjct: 481 NAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFM 540

Query: 541 NKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600
           NKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN
Sbjct: 541 NKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHN 600

Query: 601 EDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQI 660
           EDETFD+FD QSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SI+IQLCA+LPVQI
Sbjct: 601 EDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQI 660

Query: 661 DGEPWFQN-QCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRAL 720
           DGEPWFQ+  CTLVISHHG+AFMLKRAVEEPLGHAAAI+TDVLESAESNNVINA+QKR L
Sbjct: 661 DGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVL 720

Query: 721 LQEMAIRLT 728
           LQE+A RLT
Sbjct: 721 LQEIAKRLT 727

BLAST of MC03g1275 vs. ExPASy TrEMBL
Match: A0A5D3BKN2 (Diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001240 PE=3 SV=1)

HSP 1 Score: 1344 bits (3479), Expect = 0.0
Identity = 659/730 (90.27%), Postives = 692/730 (94.79%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60
           MDED EIGLLFPSWNSKNPTDRLF+ISCF AA+IGILTIAFTAFQWRRNINL WMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120
           SKRNPKKT +VPVAAHDWILESVSRGKNL+CCVCL+++SPSQTLGPMVASDSFIH C+IC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           G AAHLSCSS AQKDCKC+SMIGF+HVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKES R S GGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRMS-GGFLSS 240

Query: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSID-TGTTGSTCDMSTESTADAHHRVNGYHG 300
           ITHGANEIASSVRASIRS+ KK KH RKPS+  TG++G+  DMSTESTAD+H  VNGYHG
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDS 360
           TERNCNGS  SE +HQNGD+  K+ SN + K+SS++N KDE+HILG+ LRYEVIE+P DS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNP QVFELSS QGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQ 660
           NEDETFDNFD QSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQG+SI+IQLCA+LPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQN-QCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRA 720
           IDGEPWFQ   CTLVISHHGQAFMLKRAVEEPLGHAAAI+TDVLESAESNNVINA+QKR 
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEMAIRLT 728
           LLQEMA RLT
Sbjct: 721 LLQEMAKRLT 729

BLAST of MC03g1275 vs. TAIR 10
Match: AT5G07920.1 (diacylglycerol kinase1 )

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 538/732 (73.50%), Postives = 618/732 (84.43%), Query Frame = 0

Query: 1   MDEDSEIGLLFPSWNSKNPTD----RLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMK 60
           MD+D E+G+ FPSW SKNP D    R  + SCF+AA++GILTIA+TAFQWRRNINL W K
Sbjct: 1   MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTK 60

Query: 61  AIARSKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHC 120
           AIARSK+NPK  +KVPVA H W L+ ++R KNLNCCVCL+ MSPSQ +   VAS+SF H 
Sbjct: 61  AIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAI---VASESFFHR 120

Query: 121 CSICGAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCS 180
           C+ICGAAAH +CSS A KDCKCVSM+GFEHV+HQWAVRWTE  DQ D++SFCSYC+E CS
Sbjct: 121 CTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCS 180

Query: 181 GSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGG 240
            SFLGGSPIWCCLWCQRLVHVDCHS+M NETGDICDLG  RRLIL PLYVKE  R  SGG
Sbjct: 181 SSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGG 240

Query: 241 FLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNG 300
           FLSSITHGANE+AS+  ASIR + KK+K   + S DTG +GS CD STESTAD    VNG
Sbjct: 241 FLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNG 300

Query: 301 YHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELP 360
            H    N       +  + + D +GK     + KR+ +  QK E H L  KL+YE+ +LP
Sbjct: 301 AHAVLENSISVMNGDSSNGDSDSNGKLEKKPSVKRTGSFGQK-EYHALRSKLKYELADLP 360

Query: 361 PDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVL 420
            D+RPLLVFINKKSGA+RGDSL+QRL++ LNP QVFELSS QGPE GL+LFRKVPHF+VL
Sbjct: 361 SDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHFRVL 420

Query: 421 VCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL 480
           VCGGDGT GWVL+ I+KQNF+SPP VAILPAGTGNDL+RVLNWGGGLGSVERQGGL TVL
Sbjct: 421 VCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVL 480

Query: 481 HHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFY 540
            ++E+AAVTVLDRWKV+I++QQGKQL+ P++MNNY+G+GCDAKVAL+IHNLREENPE+FY
Sbjct: 481 QNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPERFY 540

Query: 541 NQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVD 600
           +QFMNKVLYAREGA+SIMDRTF D PWQVRVEVDGV++EVPEDAEG+LVANIGSYMGGVD
Sbjct: 541 SQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVD 600

Query: 601 LWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASL 660
           LW NEDET++NFDPQSMHDK++EVVSISGTWHLGKLQVGLSRARRLAQG ++KIQLCA L
Sbjct: 601 LWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPL 660

Query: 661 PVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQK 720
           PVQIDGEPW Q  CTL ISHHGQAFMLKRA EEPLGHAAAI+TDVLE+AE+N VINA+QK
Sbjct: 661 PVQIDGEPWNQQPCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVINASQK 720

Query: 721 RALLQEMAIRLT 729
           R LLQEMA+RLT
Sbjct: 721 RTLLQEMALRLT 728

BLAST of MC03g1275 vs. TAIR 10
Match: AT5G63770.1 (diacylglycerol kinase 2 )

HSP 1 Score: 704.1 bits (1816), Expect = 1.1e-202
Identity = 354/726 (48.76%), Postives = 476/726 (65.56%), Query Frame = 0

Query: 6   EIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIARSKRNP 65
           E+G     W   +  D  FI    +   +G+L + +T  +W++  +L W+KA AR K+  
Sbjct: 3   EVGFSLIQWLISSGADSPFIFGWLVTGSVGLLAVIYTFLKWQKKTSLNWVKAAAREKKKV 62

Query: 66  KKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSICGAAAH 125
            K  +VP++ H W  +     +   CCVCL  + P Q +    +    +H C++CG AAH
Sbjct: 63  WKRLRVPLSHHQWTDDYGYGQQPSTCCVCLYSLVPGQNVSNKASLSIPVHRCAVCGVAAH 122

Query: 126 LSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFLGGSPI 185
             CSS A KDCKCV+  G +HV H W+ RW  + D  D T+FC YC+EPC   F+  SP+
Sbjct: 123 FYCSSSAAKDCKCVAQAGSDHVRHHWSERWVNMDDNADMTAFCFYCDEPCGIPFIEASPM 182

Query: 186 WCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSSITHGA 245
           W CLWCQRL+HV CH  M  E+GD CDLGS RR+ILSP++VK +      G L++I    
Sbjct: 183 WHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRVILSPVHVKLNEANGVDGVLTTI---K 242

Query: 246 NEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGTERNCN 305
           NE+A S+R  +R K  + K+G   S++    G   + S          VNG     +   
Sbjct: 243 NELA-SIRGHVRRKRHRGKNGNGQSLN----GKLLEDSVSDPVKT--VVNGL--VVKKLR 302

Query: 306 GSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSRPLLVF 365
              + +   Q  DM     + +  +     +++++S  L    ++ +++LPPD+RPLLVF
Sbjct: 303 RDRSIDCLKQVSDM----PNAKGLQNGIGGHKRNKSAALNFMKKFSLVDLPPDARPLLVF 362

Query: 366 INKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVG 425
           IN KSG + G  L +RLNMLLNP QVFEL S QGP++GL L  KV +F+VLVCGGDGTV 
Sbjct: 363 INAKSGGQLGPFLHRRLNMLLNPVQVFELGSCQGPDAGLDLCSKVKYFRVLVCGGDGTVA 422

Query: 426 WVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVT 485
           WVL+ I+K+NF SPPPVAILP GTGNDL+RVL WG G+  V+ QG L T L  +++AAVT
Sbjct: 423 WVLDAIEKRNFESPPPVAILPLGTGNDLSRVLQWGRGISVVDGQGSLRTFLQDIDHAAVT 482

Query: 486 VLDRWKVAIVDQQGKQL---KSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNK 545
           +LDRW V IV++  ++    +  +FM NYLGIGCDAKVA + H +R+E PEKF +QF+NK
Sbjct: 483 MLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNK 542

Query: 546 VLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNED 605
           + YA+EGA+ IMDR  AD+PWQV +EVDG ++E+P+D+EG++V NIGSYMGGVDLW N+ 
Sbjct: 543 LRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDY 602

Query: 606 ETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQLCASLPVQIDG 665
           E  DNF  Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQG+ I+I + +  PVQIDG
Sbjct: 603 EHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQIDG 662

Query: 666 EPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAAIVTDVLESAESNNVINATQKRALLQE 725
           EP+ Q    L I+HHGQ FML+RA +EP GHAAAI+ +VL  AE   VINA+QK+ LLQ+
Sbjct: 663 EPFIQQPGCLEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKVLLQQ 712

Query: 726 MAIRLT 729
           MA+ L+
Sbjct: 723 MALHLS 712

BLAST of MC03g1275 vs. TAIR 10
Match: AT5G63770.2 (diacylglycerol kinase 2 )

HSP 1 Score: 586.3 bits (1510), Expect = 3.5e-167
Identity = 301/572 (52.62%), Postives = 395/572 (69.06%), Query Frame = 0

Query: 160 DQPDETSFCSYCEEPCSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRL 219
           D  D T+FC YC+EPC   F+  SP+W CLWCQRL+HV CH  M  E+GD CDLGS RR+
Sbjct: 3   DNADMTAFCFYCDEPCGIPFIEASPMWHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRV 62

Query: 220 ILSPLYVKESIRTSSGGFLSSITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGST 279
           ILSP++VK +      G L++I    NE+A S+R  +R K  + K+G   S++    G  
Sbjct: 63  ILSPVHVKLNEANGVDGVLTTI---KNELA-SIRGHVRRKRHRGKNGNGQSLN----GKL 122

Query: 280 CDMSTESTADAHHRVNGYHGTERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKD 339
            + S          VNG     +      + +   Q  DM     + +  +     ++++
Sbjct: 123 LEDSVSDPVKT--VVNGL--VVKKLRRDRSIDCLKQVSDM----PNAKGLQNGIGGHKRN 182

Query: 340 ESHILGLKLRYEVIELPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQG 399
           +S  L    ++ +++LPPD+RPLLVFIN KSG + G  L +RLNMLLNP QVFEL S QG
Sbjct: 183 KSAALNFMKKFSLVDLPPDARPLLVFINAKSGGQLGPFLHRRLNMLLNPVQVFELGSCQG 242

Query: 400 PESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNW 459
           P++GL L  KV +F+VLVCGGDGTV WVL+ I+K+NF SPPPVAILP GTGNDL+RVL W
Sbjct: 243 PDAGLDLCSKVKYFRVLVCGGDGTVAWVLDAIEKRNFESPPPVAILPLGTGNDLSRVLQW 302

Query: 460 GGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQQGKQL---KSPQFMNNYLGIGC 519
           G G+  V+ QG L T L  +++AAVT+LDRW V IV++  ++    +  +FM NYLGIGC
Sbjct: 303 GRGISVVDGQGSLRTFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGC 362

Query: 520 DAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEV 579
           DAKVA + H +R+E PEKF +QF+NK+ YA+EGA+ IMDR  AD+PWQV +EVDG ++E+
Sbjct: 363 DAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEI 422

Query: 580 PEDAEGVLVANIGSYMGGVDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHLGKLQVGL 639
           P+D+EG++V NIGSYMGGVDLW N+ E  DNF  Q MHDK LEVV + G WHLGKLQVGL
Sbjct: 423 PKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGL 482

Query: 640 SRARRLAQGQSIKIQLCASLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEEPLGHAAA 699
           S+ARRLAQG+ I+I + +  PVQIDGEP+ Q    L I+HHGQ FML+RA +EP GHAAA
Sbjct: 483 SQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQPGCLEITHHGQVFMLRRASDEPRGHAAA 542

Query: 700 IVTDVLESAESNNVINATQKRALLQEMAIRLT 729
           I+ +VL  AE   VINA+QK+ LLQ+MA+ L+
Sbjct: 543 IMNEVLLDAECKGVINASQKKVLLQQMALHLS 558

BLAST of MC03g1275 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 158.7 bits (400), Expect = 1.8e-38
Identity = 110/377 (29.18%), Postives = 169/377 (44.83%), Query Frame = 0

Query: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELS------------------SAQGPES 420
           PL+VF+N KSG R+G  +K+RL  L++  QV++L+                  +++G E 
Sbjct: 90  PLMVFVNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDEC 149

Query: 421 GLYLFRKVPHFKVLVCGGDGTVGWVLNCIDK---QNFVSPPPVAILPAGTGNDLARVLNW 480
              +  K+   +++V GGDGTVGWVL C+ +   QN +  PPV+I+P GTGNDL+R   W
Sbjct: 150 AKEIREKM---RIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGW 209

Query: 481 GGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMN---------- 540
           GG       +  +   LH    A ++ LD W + I    G+ +  P  +           
Sbjct: 210 GGSF-PFAWKSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQN 269

Query: 541 -------------------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA 600
                              NY  IG DA+VA   H+LR E P        NK++Y+  G 
Sbjct: 270 LEIEGEIPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGC 329

Query: 601 KS-------IMD---RTFADIPWQVRVEVDGVE---VEVPEDAEGVLVANIGSYMGGVDL 660
                    I D   R   +I      ++D  E   V VP+    V+  N+ SY  G + 
Sbjct: 330 SQGWFLTHCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNP 389

Query: 661 WHNEDETF---DNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSIKIQL-- 668
           W N  + +     F      D +LE+  +   WH   + V L  A+ +AQ  +I++++  
Sbjct: 390 WGNLKQDYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRG 449

BLAST of MC03g1275 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 156.8 bits (395), Expect = 6.8e-38
Identity = 108/387 (27.91%), Postives = 163/387 (42.12%), Query Frame = 0

Query: 352 VIELPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPAQVFELSSAQGPE---SGLYLFR 411
           V ++ P + P++VFIN  SG R G  LK+RL  L++  QVF+L+  +  E    GL    
Sbjct: 83  VADMAPHA-PMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEFVRYGLGCLE 142

Query: 412 KV------------PHFKVLVCGGDGTVGWVLNC---IDKQNFVSPPPVAILPAGTGNDL 471
           KV               +++V GGDGTVGWVL C   ++K      PPV ++P GTGNDL
Sbjct: 143 KVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPLGTGNDL 202

Query: 472 ARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMN---- 531
           +R   WGG      R   +   LH      V  LD WK+ +    G+ +  P  +     
Sbjct: 203 SRSFGWGGSFPFAWR-SAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPPYSLKPAEE 262

Query: 532 -------------------------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVL 591
                                    NYL IG DA+VA   H+LR   P        NK++
Sbjct: 263 NELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQGPISNKII 322

Query: 592 YAREGAKS-------IMDRTFADIPWQVRVEVDGV------EVEVPEDAEGVLVANIGSY 651
           Y+  G          + D     +   +++ +  V      E+ VP++   ++  N+ SY
Sbjct: 323 YSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSIVALNLHSY 382

Query: 652 MGGVDLWHNEDETF---DNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGQSI 672
             G   W N    +     F      D ++E+      WH   +   L  A+ +AQ  ++
Sbjct: 383 GSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAKHIAQAAAV 442

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q390170.0e+0073.50Diacylglycerol kinase 1 OS=Arabidopsis thaliana OX=3702 GN=DGK1 PE=1 SV=2[more]
Q9FFN71.6e-20148.76Diacylglycerol kinase 2 OS=Arabidopsis thaliana OX=3702 GN=DGK2 PE=1 SV=1[more]
P496214.1e-7241.36Diacylglycerol kinase beta OS=Rattus norvegicus OX=10116 GN=Dgkb PE=1 SV=1[more]
Q91WG75.4e-7240.78Diacylglycerol kinase gamma OS=Mus musculus OX=10090 GN=Dgkg PE=1 SV=1[more]
Q6NS527.0e-7238.21Diacylglycerol kinase beta OS=Mus musculus OX=10090 GN=Dgkb PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022137252.10.0100.00diacylglycerol kinase 1 isoform X1 [Momordica charantia][more]
XP_022137253.10.0100.00diacylglycerol kinase 1 isoform X2 [Momordica charantia][more]
XP_022924022.10.090.67diacylglycerol kinase 1-like [Cucurbita moschata] >XP_022924023.1 diacylglycerol... [more]
XP_023000647.10.090.67diacylglycerol kinase 1-like [Cucurbita maxima] >XP_023000648.1 diacylglycerol k... [more]
XP_023519698.10.090.40diacylglycerol kinase 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1C7R20.0100.00Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111008758 PE=3 SV=1[more]
A0A6J1C9S80.0100.00Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111008758 PE=3 SV=1[more]
A0A6J1E7Q50.090.67Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111431571 PE=3 SV=1[more]
A0A6J1KN740.090.67Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111495021 PE=3 SV=1[more]
A0A5D3BKN20.090.27Diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold24... [more]
Match NameE-valueIdentityDescription
AT5G07920.10.0e+0073.50diacylglycerol kinase1 [more]
AT5G63770.11.1e-20248.76diacylglycerol kinase 2 [more]
AT5G63770.23.5e-16752.62diacylglycerol kinase 2 [more]
AT5G57690.11.8e-3829.18diacylglycerol kinase 4 [more]
AT2G18730.16.8e-3827.91diacylglycerol kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001206Diacylglycerol kinase, catalytic domainSMARTSM00046dagk_c4a_7coord: 361..490
e-value: 1.7E-53
score: 193.7
IPR001206Diacylglycerol kinase, catalytic domainPFAMPF00781DAGK_catcoord: 361..461
e-value: 7.8E-28
score: 96.6
IPR001206Diacylglycerol kinase, catalytic domainPROSITEPS50146DAGKcoord: 357..497
score: 40.249359
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainSMARTSM00109c1_12coord: 149..211
e-value: 1.7E-6
score: 37.6
coord: 76..136
e-value: 0.0093
score: 24.9
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPFAMPF00130C1_1coord: 149..209
e-value: 5.9E-11
score: 42.1
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 75..136
score: 7.961401
IPR002219Protein kinase C-like, phorbol ester/diacylglycerol-binding domainPROSITEPS50081ZF_DAG_PE_2coord: 148..211
score: 7.9129
IPR000756Diacylglycerol kinase, accessory domainSMARTSM00045dagk_c4b_2coord: 507..664
e-value: 1.2E-78
score: 277.3
IPR000756Diacylglycerol kinase, accessory domainPFAMPF00609DAGK_acccoord: 507..664
e-value: 3.9E-55
score: 186.5
IPR017438Inorganic polyphosphate/ATP-NAD kinase, N-terminalGENE3D3.40.50.10330coord: 358..478
e-value: 7.8E-22
score: 79.2
NoneNo IPR availableGENE3D2.60.200.40coord: 505..674
e-value: 1.7E-19
score: 72.2
NoneNo IPR availableGENE3D3.30.60.20coord: 147..206
e-value: 1.7E-9
score: 39.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 310..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 310..336
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 255..284
NoneNo IPR availablePANTHERPTHR11255:SF91DIACYLGLYCEROL KINASEcoord: 17..727
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 17..727
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILY111331NAD kinase/diacylglycerol kinase-likecoord: 360..683

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g1275.1MC03g1275.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0016310 phosphorylation
biological_process GO:0007205 protein kinase C-activating G protein-coupled receptor signaling pathway
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004143 diacylglycerol kinase activity
molecular_function GO:0003951 NAD+ kinase activity
molecular_function GO:0016301 kinase activity