MC02g_new0346 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g_new0346
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H-like protein
Location	MC02: 14482749 .. 14483617 (+)
RNA-Seq Expression	MC02g_new0346
Synteny	MC02g_new0346

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCAAGTCACAGAAATTTTCAATGTCTTAAGGAATATTGTTACAGTTGGGGACATTTCTCAACCTAACAATTTAGTCGAACCTCATGATAATTCATCACTTAGGGTGGACCTACCTCTTTGTCAATCAACAGAGGGCTTCCCCTAGCACAGTTCGTCACATGAAGCTAAGCTTTCCAAGAAGGGCCAACAAGCTCCTACAAACTGTCAGGGTTGAATTCACATTCCAAGATGGACAAACATTAAAAGCCAATTACTCAGATAATTTAAAAAGTAAAGAAAAAGAGAATGTTGAAGAACCTGAAGAAATTTTTTCCAAACATGACATGGGTATGACCCACACTCAATAGAGAATGGGAAAAGAAACTGCAGGGGAAGAATATGCTCAAAGATGGGGAAAAGCAATAGAACAACTGCCAGTGCCTTTACTTAATAAAGAGACACATCCTGAAACGAATAGAAGTTCTATTAAAAGGAAAAATAAAATTCAGGCAGTAATCTCGAAGTGCAAGTGGAGGCACCAACAAGGATCTGCAGTGTACAATCCGTCTACAGTATCCATAAACTCATTGACTCTGCAGAATGGCCCACATGAAAGATCGCCAACATTACCAGACAATCATAATAGTCGGAGACAACTAAAGCGAGAGAGCTTTCATCCGATCCCAATATCTTATACCGAGTTGTTATCCCAATTATTTCAAGGTAATCTATTAGCTCTAGTACCCGTAGAGCCATTACAACCACCTTATCCAAAGTGGTACGACCCGCAAGTCCGCTGTGATTACCATGCAGGAGCTGTAGGTCATTTAACTGAGAACTGTACTTTACTAAAGCATAAAGTGCAAGTTCTAATGAAGGCATGATGGCT

mRNA sequence

ATGGGAAAAGAAACTGCAGGGGAAGAATATGCTCAAAGATGGGGAAAAGCAATAGAACAACTGCCAGTGCCTTTACTTAATAAAGAGACACATCCTGAAACGAATAGAAGTTCTATTAAAAGGAAAAATAAAATTCAGGCAGTAATCTCGAAGTGCAAGTGGAGGCACCAACAAGGATCTGCAGTGTACAATCCGTCTACAGTATCCATAAACTCATTGACTCTGCAGAATGGCCCACATGAAAGATCGCCAACATTACCAGACAATCATAATAGTCGGAGACAACTAAAGCGAGAGAGCTTTCATCCGATCCCAATATCTTATACCGAGTTGTTATCCCAATTATTTCAAGGTAATCTATTAGCTCTAGTACCCGTAGAGCCATTACAACCACCTTATCCAAAGTGGTACGACCCGCAAGTCCGCTGTGATTACCATGCAGGAGCTGTAGGTCATTTAACTGAGAACTGTACTTTACTAAAGCATAAAGTGCAAGTTCTAATGAAGGCATGA

Coding sequence (CDS)

ATGGGAAAAGAAACTGCAGGGGAAGAATATGCTCAAAGATGGGGAAAAGCAATAGAACAACTGCCAGTGCCTTTACTTAATAAAGAGACACATCCTGAAACGAATAGAAGTTCTATTAAAAGGAAAAATAAAATTCAGGCAGTAATCTCGAAGTGCAAGTGGAGGCACCAACAAGGATCTGCAGTGTACAATCCGTCTACAGTATCCATAAACTCATTGACTCTGCAGAATGGCCCACATGAAAGATCGCCAACATTACCAGACAATCATAATAGTCGGAGACAACTAAAGCGAGAGAGCTTTCATCCGATCCCAATATCTTATACCGAGTTGTTATCCCAATTATTTCAAGGTAATCTATTAGCTCTAGTACCCGTAGAGCCATTACAACCACCTTATCCAAAGTGGTACGACCCGCAAGTCCGCTGTGATTACCATGCAGGAGCTGTAGGTCATTTAACTGAGAACTGTACTTTACTAAAGCATAAAGTGCAAGTTCTAATGAAGGCATGA

Protein sequence

MGKETAGEEYAQRWGKAIEQLPVPLLNKETHPETNRSSIKRKNKIQAVISKCKWRHQQGSAVYNPSTVSINSLTLQNGPHERSPTLPDNHNSRRQLKRESFHPIPISYTELLSQLFQGNLLALVPVEPLQPPYPKWYDPQVRCDYHAGAVGHLTENCTLLKHKVQVLMKA

Homology

[Close]

BLAST of MC02g_new0346 vs. NCBI nr
Match: XP_022153553.1 (uncharacterized protein LOC111021029 [Momordica charantia])

HSP 1 Score: 162 bits (409), Expect = 3.47e-46
Identity = 81/113 (71.68%), Postives = 93/113 (82.30%), Query Frame = 0


Query: 8   EEYAQRWGKAIEQLPVPLLNKETHPETNRSSIKRKNKIQAVISKCKWRHQQGSAVYNPST 67
           E   QRWGKAIEQLPVPL+NKE HP+ N+SSIKRKNK+ AV+SK K ++QQG   Y+PST
Sbjct: 152 ENVTQRWGKAIEQLPVPLINKEPHPKMNKSSIKRKNKVLAVVSKRKRKYQQGFVAYSPST 211

Query: 68  VSINSLTLQNGPHERSPTLPDNHNSRRQLKRESFHPIPISYTELLSQLFQGNL 120
           V INS+TLQ+ P     TLP+NH+SRRQLKRESFH IP SYTELLSQLFQGNL
Sbjct: 212 VPINSMTLQDDPQ----TLPNNHSSRRQLKRESFHSIPTSYTELLSQLFQGNL 260

[Close]

BLAST of MC02g_new0346 vs. NCBI nr
Match: XP_022155098.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica charantia])

HSP 1 Score: 121 bits (303), Expect = 3.25e-28
Identity = 53/80 (66.25%), Postives = 63/80 (78.75%), Query Frame = 0


Query: 91  NSRRQLKRESFHPIPISYTELLSQLFQGNLLALVPVEPLQPPYPKWYDPQVRCDYHAGAV 150
           N+R   K+  F PIP++YTELL QLFQ N LA VPV+P+QPPYP WYD   RCDYHAGA+
Sbjct: 440 NNRSTRKQSQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPGWYDANXRCDYHAGAI 499

Query: 151 GHLTENCTLLKHKVQVLMKA 170
           GH TENCT LK++VQ L+KA
Sbjct: 500 GHSTENCTALKYRVQALIKA 519

[Close]

BLAST of MC02g_new0346 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 121 bits (303), Expect = 3.31e-28
Identity = 78/208 (37.50%), Postives = 100/208 (48.08%), Query Frame = 0


Query: 5   TAGE--EYAQRWGKAIEQLPVPLLNKETHPETNRSSIKRKNKIQAV-ISKCKWRHQQGSA 64
           T GE  EY  R G+    +  PL  K+        S K++ ++Q V   +  W+ Q  S 
Sbjct: 215 TIGERIEYGVRHGRITSTVDEPLAAKKAS-----HSKKKEGEVQMVGADRHSWKQQPYSR 274

Query: 65  V------YNPSTVSINSLTLQNGPHERSPTLPDN------------------------HN 124
                  Y P+    N   + N     SP    N                        HN
Sbjct: 275 TPRYTPYYYPTPYGYNQPFVNNATSHYSPYTFQNFRPPASQNFQPTPASQNFQPRGQQHN 334

Query: 125 S---------RRQLKRESFHPIPISYTELLSQLFQGNLLALVPVEPLQPPYPKWYDPQVR 170
           +         R   K+  F PIP++YTELL QLFQ N LA VPV+P+QPPYP+WYD   R
Sbjct: 335 TLYTQEQQTNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPRWYDTNAR 394

[Close]

BLAST of MC02g_new0346 vs. NCBI nr
Match: XP_022147189.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia])

HSP 1 Score: 119 bits (299), Expect = 1.15e-27
Identity = 52/80 (65.00%), Postives = 63/80 (78.75%), Query Frame = 0


Query: 91  NSRRQLKRESFHPIPISYTELLSQLFQGNLLALVPVEPLQPPYPKWYDPQVRCDYHAGAV 150
           N+R   K+  F PIP++YTELL QLFQ N LA VPV+P+QPPYP+WYD   RCDYHAGA+
Sbjct: 378 NNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPRWYDANARCDYHAGAI 437

Query: 151 GHLTENCTLLKHKVQVLMKA 170
            H TENCT LK++VQ L+KA
Sbjct: 438 XHSTENCTXLKYRVQALIKA 457

[Close]

BLAST of MC02g_new0346 vs. NCBI nr
Match: KAA0054657.1 (uncharacterized protein E6C27_scaffold24G004750 [Cucumis melo var. makuwa] >TYK08606.1 uncharacterized protein E5676_scaffold3734G00290 [Cucumis melo var. makuwa])

HSP 1 Score: 116 bits (290), Expect = 1.95e-27
Identity = 73/172 (42.44%), Postives = 93/172 (54.07%), Query Frame = 0


Query: 8   EEYAQRWGKAIEQLPVPLLNKETHPETNRSSIKRKNKIQAVISKCKWRHQQGSAVYNPST 67
           +EYAQRW +   Q+  PL +KE             N ++A      +    GS   N S 
Sbjct: 194 KEYAQRWRELATQVQPPLTDKELMAMFI-------NTLRAPY----YDRMVGSVSINFSD 253

Query: 68  V-----SINSLTLQNGPHERSPTLP----DNHNSRRQLKRESFHPIPISYTELLSQLFQG 127
           V      I   T  +  ++     P       N+R   K+  F PIP+SYTELL QL + 
Sbjct: 254 VITIGERIEFETNYSPSYQNRGKSPFGQATQRNARNSWKQTYFDPIPMSYTELLPQLLKS 313

Query: 128 NLLALVPVEPLQPPYPKWYDPQVRCDYHAGAVGHLTENCTLLKHKVQVLMKA 170
           + +A+VP EPLQPPYPKWYDP V+ +YHAGAVGH TENC  LK KVQ L+KA
Sbjct: 314 HQVAIVPQEPLQPPYPKWYDPNVKREYHAGAVGHSTENCFPLKAKVQSLVKA 354

[Close]

BLAST of MC02g_new0346 vs. ExPASy TrEMBL
Match: A0A6J1DHS7 (uncharacterized protein LOC111021029 OS=Momordica charantia OX=3673 GN=LOC111021029 PE=4 SV=1)

HSP 1 Score: 162 bits (409), Expect = 1.68e-46
Identity = 81/113 (71.68%), Postives = 93/113 (82.30%), Query Frame = 0


Query: 8   EEYAQRWGKAIEQLPVPLLNKETHPETNRSSIKRKNKIQAVISKCKWRHQQGSAVYNPST 67
           E   QRWGKAIEQLPVPL+NKE HP+ N+SSIKRKNK+ AV+SK K ++QQG   Y+PST
Sbjct: 152 ENVTQRWGKAIEQLPVPLINKEPHPKMNKSSIKRKNKVLAVVSKRKRKYQQGFVAYSPST 211

Query: 68  VSINSLTLQNGPHERSPTLPDNHNSRRQLKRESFHPIPISYTELLSQLFQGNL 120
           V INS+TLQ+ P     TLP+NH+SRRQLKRESFH IP SYTELLSQLFQGNL
Sbjct: 212 VPINSMTLQDDPQ----TLPNNHSSRRQLKRESFHSIPTSYTELLSQLFQGNL 260

[Close]

BLAST of MC02g_new0346 vs. ExPASy TrEMBL
Match: A0A6J1DM29 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia OX=3673 GN=LOC111022231 PE=4 SV=1)

HSP 1 Score: 121 bits (303), Expect = 1.57e-28
Identity = 53/80 (66.25%), Postives = 63/80 (78.75%), Query Frame = 0


Query: 91  NSRRQLKRESFHPIPISYTELLSQLFQGNLLALVPVEPLQPPYPKWYDPQVRCDYHAGAV 150
           N+R   K+  F PIP++YTELL QLFQ N LA VPV+P+QPPYP WYD   RCDYHAGA+
Sbjct: 440 NNRSTRKQSQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPGWYDANXRCDYHAGAI 499

Query: 151 GHLTENCTLLKHKVQVLMKA 170
           GH TENCT LK++VQ L+KA
Sbjct: 500 GHSTENCTALKYRVQALIKA 519

[Close]

BLAST of MC02g_new0346 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 121 bits (303), Expect = 1.60e-28
Identity = 78/208 (37.50%), Postives = 100/208 (48.08%), Query Frame = 0


Query: 5   TAGE--EYAQRWGKAIEQLPVPLLNKETHPETNRSSIKRKNKIQAV-ISKCKWRHQQGSA 64
           T GE  EY  R G+    +  PL  K+        S K++ ++Q V   +  W+ Q  S 
Sbjct: 215 TIGERIEYGVRHGRITSTVDEPLAAKKAS-----HSKKKEGEVQMVGADRHSWKQQPYSR 274

Query: 65  V------YNPSTVSINSLTLQNGPHERSPTLPDN------------------------HN 124
                  Y P+    N   + N     SP    N                        HN
Sbjct: 275 TPRYTPYYYPTPYGYNQPFVNNATSHYSPYTFQNFRPPASQNFQPTPASQNFQPRGQQHN 334

Query: 125 S---------RRQLKRESFHPIPISYTELLSQLFQGNLLALVPVEPLQPPYPKWYDPQVR 170
           +         R   K+  F PIP++YTELL QLFQ N LA VPV+P+QPPYP+WYD   R
Sbjct: 335 TLYTQEQQTNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPRWYDTNAR 394

[Close]

BLAST of MC02g_new0346 vs. ExPASy TrEMBL
Match: A0A6J1D099 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1)

HSP 1 Score: 119 bits (299), Expect = 5.58e-28
Identity = 52/80 (65.00%), Postives = 63/80 (78.75%), Query Frame = 0


Query: 91  NSRRQLKRESFHPIPISYTELLSQLFQGNLLALVPVEPLQPPYPKWYDPQVRCDYHAGAV 150
           N+R   K+  F PIP++YTELL QLFQ N LA VPV+P+QPPYP+WYD   RCDYHAGA+
Sbjct: 378 NNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPYPRWYDANARCDYHAGAI 437

Query: 151 GHLTENCTLLKHKVQVLMKA 170
            H TENCT LK++VQ L+KA
Sbjct: 438 XHSTENCTXLKYRVQALIKA 457

[Close]

BLAST of MC02g_new0346 vs. ExPASy TrEMBL
Match: A0A5A7UJ84 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3734G00290 PE=4 SV=1)

HSP 1 Score: 116 bits (290), Expect = 9.44e-28
Identity = 73/172 (42.44%), Postives = 93/172 (54.07%), Query Frame = 0


Query: 8   EEYAQRWGKAIEQLPVPLLNKETHPETNRSSIKRKNKIQAVISKCKWRHQQGSAVYNPST 67
           +EYAQRW +   Q+  PL +KE             N ++A      +    GS   N S 
Sbjct: 194 KEYAQRWRELATQVQPPLTDKELMAMFI-------NTLRAPY----YDRMVGSVSINFSD 253

Query: 68  V-----SINSLTLQNGPHERSPTLP----DNHNSRRQLKRESFHPIPISYTELLSQLFQG 127
           V      I   T  +  ++     P       N+R   K+  F PIP+SYTELL QL + 
Sbjct: 254 VITIGERIEFETNYSPSYQNRGKSPFGQATQRNARNSWKQTYFDPIPMSYTELLPQLLKS 313

Query: 128 NLLALVPVEPLQPPYPKWYDPQVRCDYHAGAVGHLTENCTLLKHKVQVLMKA 170
           + +A+VP EPLQPPYPKWYDP V+ +YHAGAVGH TENC  LK KVQ L+KA
Sbjct: 314 HQVAIVPQEPLQPPYPKWYDPNVKREYHAGAVGHSTENCFPLKAKVQSLVKA 354

The following BLAST results are available for this feature:

BLAST of MC02g_new0346 vs.


Match Name	E-value	Identity	Description

BLAST of MC02g_new0346 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153553.1	3.47e-46	71.68	uncharacterized protein LOC111021029 [Momordica charantia]	[more]
XP_022155098.1	3.25e-28	66.25	LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica ch...	[more]
XP_022158986.1	3.31e-28	37.50	LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia]	[more]
XP_022147189.1	1.15e-27	65.00	LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia]	[more]
KAA0054657.1	1.95e-27	42.44	uncharacterized protein E6C27_scaffold24G004750 [Cucumis melo var. makuwa] >TYK0...	[more]

BLAST of MC02g_new0346 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHS7	1.68e-46	71.68	uncharacterized protein LOC111021029 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1DM29	1.57e-28	66.25	LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia...	[more]
A0A6J1E2J7	1.60e-28	37.50	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1	[more]
A0A6J1D099	5.58e-28	65.00	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1	[more]
A0A5A7UJ84	9.44e-28	42.44	Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of MC02g_new0346 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 72..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g_new0346.1	MC02g_new0346.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0015074	DNA integration
biological_process	GO:0006310	DNA recombination
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity