MC01g0490 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g0490
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionLOW QUALITY PROTEIN: probable membrane-associated kinase regulator 4
LocationMC01: 11369669 .. 11370778 (-)
RNA-Seq ExpressionMC01g0490
SyntenyMC01g0490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCGCCAAAGCCTCACTCGCCGCCCCTCCCCCGGAAGAAGACTACATTGACATGGAACTCACCTCTTCATCTTCTTCTTCCTCCAATTTATTCTGCTATTCCATCGAATCCCCACCGCAGTCCAGAGATTTCGAGTTCCAATTCCAGATGACGTCATTTTCCGGCCAAACAGAGCCGGCAACTTCGCCGGCCGACGAGCTCTTCTACAAGGGAAAATTGCTCCCCCTCCACCTCCCTCCCCGTTTACAAATGGTCCAAAAGCTCCTCCACAGCCCCTCCTCCCAAAACCCATCAATAAAAGAACAATCTTTTTCAGAAAATTTCCCAATTCCTTTAATTTCCATTGCAGCACCTCTCGAGTCGGGCAGTTTCTCACCCACTGAGTCGTGCAGAGCGAGTTGCGAGCTGAACCCAGACGAGTATTTGGTGGGATGGAGAGGGGAAGAAATCAAGGGCTTTTTGGGGGACCCTCCAAGAAAATCATGGTCTAAAAAAATGAAGCAATTGAAGAATTCTTCACAGGCTTATTTCAAGGCCATGTTTGGGAAATCTGGCTGTTCAAACAAGTCCTCTGCTAAATCTGTTTTCAATGAAGAAACAGCAGAGGGCGTTTTGGGAAGTTCAGGGCAGTGTGATAAAGTTGTGAAGAAGAACCCATTTGGGAAAATCGATTATAACAAATGGAAGATGCCAAACAGTGGCTTAGAGAAGGAGAAAATTGAGGAATTTTATGGTGGGATTTTGAATAACCATAGAAGGTCATTTTCAGGGGCGATTCAGAGGAATTCTTCTGCAACTAACAATAAATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAGCTTCATCCTCTGGTTCTTCTTCTTTTTCTTTCAGTTCAAATGGGTTTTCTGATCTGCAGTTGTTTAAGAGAAGTATCAGTGAAAATTCAGAGATTGAGAGCTCAATTGAAGGGGCAATTGCCCACTGTAAAGAGTCTCAGAAGCTGTTTGGAAGTTCAAAGAAAAATGAAAGGGAATCTCAAGCAGAAACTTGTTCTCTATCTGCTTCAAAGATTTTCTTGCCTTGTGGGGATCAGAAAAGTTCAGGACTTTGCTCCATT

mRNA sequence

ATGGCCACCGCCAAAGCCTCACTCGCCGCCCCTCCCCCGGAAGAAGACTACATTGACATGGAACTCACCTCTTCATCTTCTTCTTCCTCCAATTTATTCTGCTATTCCATCGAATCCCCACCGCAGTCCAGAGATTTCGAGTTCCAATTCCAGATGACGTCATTTTCCGGCCAAACAGAGCCGGCAACTTCGCCGGCCGACGAGCTCTTCTACAAGGGAAAATTGCTCCCCCTCCACCTCCCTCCCCGTTTACAAATGGTCCAAAAGCTCCTCCACAGCCCCTCCTCCCAAAACCCATCAATAAAAGAACAATCTTTTTCAGAAAATTTCCCAATTCCTTTAATTTCCATTGCAGCACCTCTCGAGTCGGGCAGTTTCTCACCCACTGAGTCGTGCAGAGCGAGTTGCGAGCTGAACCCAGACGAGTATTTGGTGGGATGGAGAGGGGAAGAAATCAAGGGCTTTTTGGGGGACCCTCCAAGAAAATCATGGTCTAAAAAAATGAAGCAATTGAAGAATTCTTCACAGGCTTATTTCAAGGCCATGTTTGGGAAATCTGGCTGTTCAAACAAGTCCTCTGCTAAATCTGTTTTCAATGAAGAAACAGCAGAGGGCGTTTTGGGAAGTTCAGGGCAGTGTGATAAAGTTGTGAAGAAGAACCCATTTGGGAAAATCGATTATAACAAATGGAAGATGCCAAACAGTGGCTTAGAGAAGGAGAAAATTGAGGAATTTTATGGTGGGATTTTGAATAACCATAGAAGGTCATTTTCAGGGGCGATTCAGAGGAATTCTTCTGCAACTAACAATAAATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAGCTTCATCCTCTGGTTCTTCTTCTTTTTCTTTCAGTTCAAATGGGTTTTCTGATCTGCAGTTGTTTAAGAGAAGTATCAGTGAAAATTCAGAGATTGAGAGCTCAATTGAAGGGGCAATTGCCCACTGTAAAGAGTCTCAGAAGCTGTTTGGAAGTTCAAAGAAAAATGAAAGGGAATCTCAAGCAGAAACTTGTTCTCTATCTGCTTCAAAGATTTTCTTGCCTTGTGGGGATCAGAAAAGTTCAGGACTTTGCTCCATT

Coding sequence (CDS)

ATGGCCACCGCCAAAGCCTCACTCGCCGCCCCTCCCCCGGAAGAAGACTACATTGACATGGAACTCACCTCTTCATCTTCTTCTTCCTCCAATTTATTCTGCTATTCCATCGAATCCCCACCGCAGTCCAGAGATTTCGAGTTCCAATTCCAGATGACGTCATTTTCCGGCCAAACAGAGCCGGCAACTTCGCCGGCCGACGAGCTCTTCTACAAGGGAAAATTGCTCCCCCTCCACCTCCCTCCCCGTTTACAAATGGTCCAAAAGCTCCTCCACAGCCCCTCCTCCCAAAACCCATCAATAAAAGAACAATCTTTTTCAGAAAATTTCCCAATTCCTTTAATTTCCATTGCAGCACCTCTCGAGTCGGGCAGTTTCTCACCCACTGAGTCGTGCAGAGCGAGTTGCGAGCTGAACCCAGACGAGTATTTGGTGGGATGGAGAGGGGAAGAAATCAAGGGCTTTTTGGGGGACCCTCCAAGAAAATCATGGTCTAAAAAAATGAAGCAATTGAAGAATTCTTCACAGGCTTATTTCAAGGCCATGTTTGGGAAATCTGGCTGTTCAAACAAGTCCTCTGCTAAATCTGTTTTCAATGAAGAAACAGCAGAGGGCGTTTTGGGAAGTTCAGGGCAGTGTGATAAAGTTGTGAAGAAGAACCCATTTGGGAAAATCGATTATAACAAATGGAAGATGCCAAACAGTGGCTTAGAGAAGGAGAAAATTGAGGAATTTTATGGTGGGATTTTGAATAACCATAGAAGGTCATTTTCAGGGGCGATTCAGAGGAATTCTTCTGCAACTAACAATAAATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAGCTTCATCCTCTGGTTCTTCTTCTTTTTCTTTCAGTTCAAATGGGTTTTCTGATCTGCAGTTGTTTAAGAGAAGTATCAGTGAAAATTCAGAGATTGAGAGCTCAATTGAAGGGGCAATTGCCCACTGTAAAGAGTCTCAGAAGCTGTTTGGAAGTTCAAAGAAAAATGAAAGGGAATCTCAAGCAGAAACTTGTTCTCTATCTGCTTCAAAGATTTTCTTGCCTTGTGGGGATCAGAAAAGTTCAGGACTTTGCTCCATT

Protein sequence

MATAKASLAAPPPEEDYIDMELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSGQTEPATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSSQAYFKAMFGKSGCSNKSSAKSVFNEETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIEEFYGGILNNHRRSFSGAIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPCGDQKSSGLCSI
Homology
BLAST of MC01g0490 vs. ExPASy Swiss-Prot
Match: O80624 (Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 GN=MAKR4 PE=3 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.4e-38
Identity = 130/352 (36.93%), Postives = 188/352 (53.41%), Query Frame = 0

Query: 14  EEDYIDMELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSG-QTEPATSPADELFYK 73
           EEDYIDME+T    S +NL   ++ +   +   EF+FQM+     + +  TSPADELFYK
Sbjct: 12  EEDYIDMEVT----SFTNLVRKTLSN---NYPREFEFQMSHLCPLEIDKTTSPADELFYK 71

Query: 74  GKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESC 133
           GKLLPLHLPPRLQMVQK+L   +  +        +     P+ S   P ES + SP ESC
Sbjct: 72  GKLLPLHLPPRLQMVQKILEDYTFDDEFYSTPLATGTVTTPVTS-NTPFESCTVSPAESC 131

Query: 134 RASCELNPDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNS--------SQAYFKAMFG 193
           + S ELNP++Y + +     +    D  +KSW+ K++ +K S        S+AY ++ FG
Sbjct: 132 QVSKELNPEDYFLEYSDSLEE---DDEKKKSWTTKLRLMKQSSLGTKIKASRAYLRSFFG 191

Query: 194 KSGCSNKSS-AKSVFNEETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIE 253
           K+ CS++SS A S       + VL  S       +  PFG+I   + K  ++        
Sbjct: 192 KTSCSDESSCASSAARVADEDSVLRYS-------RVKPFGQIKTERPKKQSN-------- 251

Query: 254 EFYGGILNNHRRSFSGAIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQ 313
              G +  +HRRSFS +++R ++ ++N  SS+S                     GF  LQ
Sbjct: 252 ---GSVSGSHRRSFSVSMRRQAAKSSNNKSSNSL--------------------GFRPLQ 311

Query: 314 LFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQAETCSLSASKI 356
             KRS S +SEIE+SI+GAI HCK+SQ+     K+    ++   CSLSAS+I
Sbjct: 312 FLKRSTSSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRI 314

BLAST of MC01g0490 vs. ExPASy Swiss-Prot
Match: Q9ZUS8 (Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 GN=MAKR3 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.3e-34
Identity = 149/362 (41.16%), Postives = 186/362 (51.38%), Query Frame = 0

Query: 14  EEDYIDMEL-----TSSSSSSSNLFCYSI-ESPPQSRDFEFQFQMTSFSGQTEPATSPAD 73
           ++ YIDME+     +SSS+SSS+ F + +  SPPQSR+FEFQ   +S     E  TSPAD
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQ-MCSSAVASGESTTSPAD 77

Query: 74  ELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFS 133
           ELFYKG+LLPLHLPPRL+MVQKLL + SS   + +        PI   + A  L    FS
Sbjct: 78  ELFYKGQLLPLHLPPRLKMVQKLLLASSSSTAATET-------PISPRAAADVLSPRRFS 137

Query: 134 PTESCRASCELNPDEYLVGWRGEEIKGFLGDPPR---KSWSKKMK-----QLKNSSQAYF 193
                  SCE+  DE        E+K F+         SWSKK+K     Q   +S+AY 
Sbjct: 138 -------SCEIGQDENCFFEISTELKRFIESNENHLGNSWSKKIKHSSITQKLKASRAYI 197

Query: 194 KAMFGKSGCSNKSSAKSVFNEETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEK 253
           KA+F K  CS+ S     F  E ++             KKNPF   +             
Sbjct: 198 KALFSKQACSDSSEINPRFKIEPSK----------VSRKKNPFVNSE------------- 257

Query: 254 EKIEEFYGGILNNHRRSFSGAIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGF 313
                     L  HRRSFSG IQR+S A        S+SSSSSS++SS SSSFSF SNG 
Sbjct: 258 --------NPLLIHRRSFSGVIQRHSQA------KCSTSSSSSSSASSLSSSFSFGSNGS 317

Query: 314 SDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPC 362
            DLQ   RS   ++  ++SIEGAI HCK+S   F + K N  ES  E CS   S     C
Sbjct: 318 LDLQTLMRS---SNASDNSIEGAIEHCKQS---FTTRKSNVTES--ELCSSRTS--VSTC 317

BLAST of MC01g0490 vs. NCBI nr
Match: XP_022143838.1 (probable membrane-associated kinase regulator 3 [Momordica charantia])

HSP 1 Score: 657 bits (1694), Expect = 9.55e-237
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 20  MELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSGQTEPATSPADELFYKGKLLPLH 79
           MELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSGQTEPATSPADELFYKGKLLPLH
Sbjct: 1   MELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSGQTEPATSPADELFYKGKLLPLH 60

Query: 80  LPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESCRASCELN 139
           LPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESCRASCELN
Sbjct: 61  LPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESCRASCELN 120

Query: 140 PDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSSQAYFKAMFGKSGCSNKSSAKSVFN 199
           PDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSSQAYFKAMFGKSGCSNKSSAKSVFN
Sbjct: 121 PDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSSQAYFKAMFGKSGCSNKSSAKSVFN 180

Query: 200 EETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIEEFYGGILNNHRRSFSG 259
           EETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIEEFYGGILNNHRRSFSG
Sbjct: 181 EETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIEEFYGGILNNHRRSFSG 240

Query: 260 AIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSI 319
           AIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSI
Sbjct: 241 AIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSI 300

Query: 320 EGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPCGDQKSSGLCSI 370
           EGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPCGDQKSSGLCSI
Sbjct: 301 EGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPCGDQKSSGLCSI 351

BLAST of MC01g0490 vs. NCBI nr
Match: XP_038883185.1 (probable membrane-associated kinase regulator 4 [Benincasa hispida])

HSP 1 Score: 452 bits (1162), Expect = 4.20e-155
Identity = 283/398 (71.11%), Postives = 313/398 (78.64%), Query Frame = 0

Query: 1   MATAKASLAAPPPEEDYIDMELTSSSSSSSNLFCYSIESP-PQS-------RDFEFQFQM 60
           MATA+A  AA P EEDYIDMELTSS SS  N FCYSI SP P S       RDFEFQFQM
Sbjct: 1   MATAEAPFAALPAEEDYIDMELTSSPSS--NSFCYSIRSPTPPSPPAASAARDFEFQFQM 60

Query: 61  TSFSGQTEPATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPI 120
           TSFS +TE  TSPAD+LFYKGKLLPLHLPPRLQMVQKLLHSP SQ+PS K +SFSENF +
Sbjct: 61  TSFSDETEAITSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPKSQDPSRKTESFSENFEM 120

Query: 121 PLISIAAPL-ESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFL--GDPPR-KSWSKKM 180
           P I+I AP  ES + SP+ESCRASCELNPD+YL+G+RGE IK F+   DPPR KSWSKK+
Sbjct: 121 PFITIKAPPHESCNVSPSESCRASCELNPDDYLLGFRGEIIKDFVVGRDPPRTKSWSKKV 180

Query: 181 KQLKNSSQAYFKAMFGKSGCS----NKSSAKSVFNEETAEGVLG--SSGQCDKVVKKNPF 240
           KQLK SSQAYFKA+FGKS CS    NKSS  SVFNEE+        SSG+  KV KK PF
Sbjct: 181 KQLKQSSQAYFKALFGKSACSTSTNNKSSVNSVFNEESQSIFESERSSGKFIKVSKKAPF 240

Query: 241 GKIDYNKWKMPNSGLEKEKIEEFYGGILN--NHRRSFSGAIQRNSSAT------NNKSSS 300
           G+IDYNKW++PN+ LEKEK EEFYGGILN  NHRRSFSGAIQR SS++      NNKSSS
Sbjct: 241 GRIDYNKWQIPNNALEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSTNNNSNNNKSSS 300

Query: 301 SSSS--SSSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKL 360
           S SS  S+SSS SSS SSSFSFSSNGF DLQ FKRSIS NSE E+SIEGAIAHCK+SQKL
Sbjct: 301 SCSSCSSTSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSERENSIEGAIAHCKQSQKL 360

Query: 361 FGSSKKNERESQAETCSLSASKIFLPCGDQKSSGLCSI 370
             +SKKNE E+Q +TCSLS SK+ LPCGDQKSS LC I
Sbjct: 361 IDNSKKNESETQPQTCSLSVSKV-LPCGDQKSSQLCRI 395

BLAST of MC01g0490 vs. NCBI nr
Match: KGN54211.1 (hypothetical protein Csa_018049 [Cucumis sativus])

HSP 1 Score: 447 bits (1151), Expect = 1.34e-153
Identity = 272/389 (69.92%), Postives = 309/389 (79.43%), Query Frame = 0

Query: 1   MATAKASLAAPPPEEDYIDMELTSSSSSSSNLFCYSIESPPQ-------SRDFEFQFQMT 60
           MATA A  A+ P EEDYIDMELTSS SS S  FCYSI SPP        +RDFEFQFQMT
Sbjct: 1   MATANAPFASAPAEEDYIDMELTSSPSSKS--FCYSIGSPPPPPPPPQAARDFEFQFQMT 60

Query: 61  SFSGQTEPATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIP 120
           SFSG+TE  TSPAD+LFYKGKLLPLHLPPRLQMVQKLLHSP+SQNPS K +SFSENF IP
Sbjct: 61  SFSGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIP 120

Query: 121 LISIAAPL-ESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFLGDPPR--KSWSKKMKQ 180
            ISI AP  ES + SP+ESCR SCELNPDE+L+G+RGE IK F+       KSWSKK+KQ
Sbjct: 121 FISIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTATTKSWSKKVKQ 180

Query: 181 LKNSSQAYFKAMFGKSGCSNKSSAKSVFNEETAEGVLGS---SGQCDKVVKKNPFGKIDY 240
           LK SSQAYFK++FGKS CS+ + + SVFNEET E +  +   SG+  KV KK PFG+IDY
Sbjct: 181 LKQSSQAYFKSLFGKSVCSSNNRS-SVFNEET-ENIFENRRNSGKFIKVSKKTPFGRIDY 240

Query: 241 NKWKMPNSGLEKEKIEEFYGGILN--NHRRSFSGAIQR----NSSATNNKSSSSSSSSSS 300
           NKW++PN  LEKEK EEFYGGILN  NHRRSFSGAIQR    +SS+TNN + +SSS S+S
Sbjct: 241 NKWQIPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKTSSSCSTS 300

Query: 301 SSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNER 360
           SS SSS SSSFSFSSNGF DLQ FKRSIS NSEIE+SIEGAIAHCK+SQK+  +S+KNE 
Sbjct: 301 SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNES 360

Query: 361 ESQAETCSLSASKIFLPCGDQKSSGLCSI 370
           ESQ +TCSLS SK+ LPCGDQK+S LC I
Sbjct: 361 ESQLQTCSLSVSKV-LPCGDQKTSQLCRI 384

BLAST of MC01g0490 vs. NCBI nr
Match: XP_004142216.2 (probable membrane-associated kinase regulator 4 [Cucumis sativus])

HSP 1 Score: 400 bits (1027), Expect = 1.69e-135
Identity = 239/385 (62.08%), Postives = 272/385 (70.65%), Query Frame = 0

Query: 1   MATAKASLAAPPPEEDYIDMELTSSSSSSSNLFCYSIESPPQ-------SRDFEFQFQMT 60
           MATA A  A+ P EEDYIDMELTSS SS S  FCYSI SPP        +RDFEFQFQMT
Sbjct: 1   MATANAPFASAPAEEDYIDMELTSSPSSKS--FCYSIGSPPPPPPPPQAARDFEFQFQMT 60

Query: 61  SFSGQTEPATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIP 120
           SFSG+TE  TSPAD+LFYKGKLLPLHLPPRLQMVQKLLHSP+SQNPS K +SFSENF IP
Sbjct: 61  SFSGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIP 120

Query: 121 LISIAAPL-ESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFLGDPPR--KSWSKKMKQ 180
            ISI AP  ES + SP+ESCR SCELNPDE+L+G+RGE IK F+       KSWSKK+KQ
Sbjct: 121 FISIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTATTKSWSKKVKQ 180

Query: 181 LKNSSQAYFKAMFGKSGCSNKSSAKSVFNEETAEGVLGS---SGQCDKVVKKNPFGKIDY 240
           LK SSQAYFK++FGKS CS+ + + SVFNEET E +  +   SG+  KV KK PFG+IDY
Sbjct: 181 LKQSSQAYFKSLFGKSVCSSNNRS-SVFNEET-ENIFENRRNSGKFIKVSKKTPFGRIDY 240

Query: 241 NKWKMPNSGLEKEKIEEFYGGILN--NHRRSFSGAIQRNSSATNNKSSSSSSSSSSSSAS 300
           NKW++PN  LEKEK EEFYGGILN  NHRRSFSG+                         
Sbjct: 241 NKWQIPNQTLEKEKAEEFYGGILNINNHRRSFSGS------------------------- 300

Query: 301 SSGSSSFSFSSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQA 360
                              FKRSIS NSEIE+SIEGAIAHCK+SQK+  +S+KNE ESQ 
Sbjct: 301 -------------------FKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNESESQL 336

Query: 361 ETCSLSASKIFLPCGDQKSSGLCSI 370
           +TCSLS SK+ LPCGDQK+S LC I
Sbjct: 361 QTCSLSVSKV-LPCGDQKTSQLCRI 336

BLAST of MC01g0490 vs. NCBI nr
Match: XP_022978386.1 (probable membrane-associated kinase regulator 4 isoform X1 [Cucurbita maxima])

HSP 1 Score: 396 bits (1018), Expect = 9.59e-134
Identity = 246/375 (65.60%), Postives = 279/375 (74.40%), Query Frame = 0

Query: 1   MATAKASLAAPPPEEDYIDMELTSSSSSSSNLFCYSIESPPQ----SRDFEFQFQMTSFS 60
           MAT +A   A P EEDYIDMELTSS SS  N F YSI SPP     +RDFEFQ QMTSFS
Sbjct: 1   MATTEAPFTAAPAEEDYIDMELTSSPSS--NSFSYSIRSPPPPLPPARDFEFQIQMTSFS 60

Query: 61  GQTEPATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLIS 120
           G+TEP TSPAD+LFYKGKLLPLHLPPRLQMVQ LLHSP+SQNPS K +SF EN  IP I+
Sbjct: 61  GETEPTTSPADDLFYKGKLLPLHLPPRLQMVQNLLHSPNSQNPSRKNKSFPENLQIPFIA 120

Query: 121 IAAPL-ESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSS 180
           I AP  ES +FSPTESCRASCELNPDEYL+ +RGE IK F+G+PPRKSWS K+KQLK SS
Sbjct: 121 IKAPPHESCNFSPTESCRASCELNPDEYLLRFRGE-IKDFVGNPPRKSWSNKVKQLKQSS 180

Query: 181 QAYFKAMFGKSGCSNKSSAKSVFNEETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNS 240
           QAYFKA+FGKS CS+KS    +FNEET E V  +     K + K PFG+IDYNKWK+ ++
Sbjct: 181 QAYFKALFGKSPCSSKS----LFNEET-ECVFKNRVSSWKFINKTPFGRIDYNKWKIQST 240

Query: 241 GLEKEKIEEFYGGILN---NHRRSFSGAIQRNSSATNNKSSSSSSS---SSSSSASSSGS 300
            +EKEK EEF    LN   NHRRSFSGAIQ  SS+ ++ SS SS S   SSSSS SSS S
Sbjct: 241 FIEKEKTEEFSSSSLNMNNNHRRSFSGAIQTASSSCSSCSSCSSCSCSCSSSSSGSSSLS 300

Query: 301 SSFSFSSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQAETCS 360
           SSFSF           KRS S NSEIE SIEGAIAHCK+SQK+  +S+K E ES  ETC 
Sbjct: 301 SSFSF-----------KRSNSANSEIERSIEGAIAHCKQSQKVLDNSRKREAESSPETCG 356

Query: 361 LSASKIFLPCGDQKS 364
           +S SK+ LPCG+QKS
Sbjct: 361 VSVSKVVLPCGNQKS 356

BLAST of MC01g0490 vs. ExPASy TrEMBL
Match: A0A6J1CRQ3 (probable membrane-associated kinase regulator 3 OS=Momordica charantia OX=3673 GN=LOC111013657 PE=4 SV=1)

HSP 1 Score: 657 bits (1694), Expect = 4.62e-237
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 20  MELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSGQTEPATSPADELFYKGKLLPLH 79
           MELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSGQTEPATSPADELFYKGKLLPLH
Sbjct: 1   MELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSGQTEPATSPADELFYKGKLLPLH 60

Query: 80  LPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESCRASCELN 139
           LPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESCRASCELN
Sbjct: 61  LPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESCRASCELN 120

Query: 140 PDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSSQAYFKAMFGKSGCSNKSSAKSVFN 199
           PDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSSQAYFKAMFGKSGCSNKSSAKSVFN
Sbjct: 121 PDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSSQAYFKAMFGKSGCSNKSSAKSVFN 180

Query: 200 EETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIEEFYGGILNNHRRSFSG 259
           EETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIEEFYGGILNNHRRSFSG
Sbjct: 181 EETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIEEFYGGILNNHRRSFSG 240

Query: 260 AIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSI 319
           AIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSI
Sbjct: 241 AIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSI 300

Query: 320 EGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPCGDQKSSGLCSI 370
           EGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPCGDQKSSGLCSI
Sbjct: 301 EGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPCGDQKSSGLCSI 351

BLAST of MC01g0490 vs. ExPASy TrEMBL
Match: A0A0A0L234 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G293210 PE=4 SV=1)

HSP 1 Score: 447 bits (1151), Expect = 6.49e-154
Identity = 272/389 (69.92%), Postives = 309/389 (79.43%), Query Frame = 0

Query: 1   MATAKASLAAPPPEEDYIDMELTSSSSSSSNLFCYSIESPPQ-------SRDFEFQFQMT 60
           MATA A  A+ P EEDYIDMELTSS SS S  FCYSI SPP        +RDFEFQFQMT
Sbjct: 1   MATANAPFASAPAEEDYIDMELTSSPSSKS--FCYSIGSPPPPPPPPQAARDFEFQFQMT 60

Query: 61  SFSGQTEPATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIP 120
           SFSG+TE  TSPAD+LFYKGKLLPLHLPPRLQMVQKLLHSP+SQNPS K +SFSENF IP
Sbjct: 61  SFSGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIP 120

Query: 121 LISIAAPL-ESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFLGDPPR--KSWSKKMKQ 180
            ISI AP  ES + SP+ESCR SCELNPDE+L+G+RGE IK F+       KSWSKK+KQ
Sbjct: 121 FISIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTATTKSWSKKVKQ 180

Query: 181 LKNSSQAYFKAMFGKSGCSNKSSAKSVFNEETAEGVLGS---SGQCDKVVKKNPFGKIDY 240
           LK SSQAYFK++FGKS CS+ + + SVFNEET E +  +   SG+  KV KK PFG+IDY
Sbjct: 181 LKQSSQAYFKSLFGKSVCSSNNRS-SVFNEET-ENIFENRRNSGKFIKVSKKTPFGRIDY 240

Query: 241 NKWKMPNSGLEKEKIEEFYGGILN--NHRRSFSGAIQR----NSSATNNKSSSSSSSSSS 300
           NKW++PN  LEKEK EEFYGGILN  NHRRSFSGAIQR    +SS+TNN + +SSS S+S
Sbjct: 241 NKWQIPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKTSSSCSTS 300

Query: 301 SSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNER 360
           SS SSS SSSFSFSSNGF DLQ FKRSIS NSEIE+SIEGAIAHCK+SQK+  +S+KNE 
Sbjct: 301 SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNES 360

Query: 361 ESQAETCSLSASKIFLPCGDQKSSGLCSI 370
           ESQ +TCSLS SK+ LPCGDQK+S LC I
Sbjct: 361 ESQLQTCSLSVSKV-LPCGDQKTSQLCRI 384

BLAST of MC01g0490 vs. ExPASy TrEMBL
Match: A0A6J1IMI8 (probable membrane-associated kinase regulator 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478391 PE=4 SV=1)

HSP 1 Score: 396 bits (1018), Expect = 4.64e-134
Identity = 246/375 (65.60%), Postives = 279/375 (74.40%), Query Frame = 0

Query: 1   MATAKASLAAPPPEEDYIDMELTSSSSSSSNLFCYSIESPPQ----SRDFEFQFQMTSFS 60
           MAT +A   A P EEDYIDMELTSS SS  N F YSI SPP     +RDFEFQ QMTSFS
Sbjct: 1   MATTEAPFTAAPAEEDYIDMELTSSPSS--NSFSYSIRSPPPPLPPARDFEFQIQMTSFS 60

Query: 61  GQTEPATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLIS 120
           G+TEP TSPAD+LFYKGKLLPLHLPPRLQMVQ LLHSP+SQNPS K +SF EN  IP I+
Sbjct: 61  GETEPTTSPADDLFYKGKLLPLHLPPRLQMVQNLLHSPNSQNPSRKNKSFPENLQIPFIA 120

Query: 121 IAAPL-ESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSS 180
           I AP  ES +FSPTESCRASCELNPDEYL+ +RGE IK F+G+PPRKSWS K+KQLK SS
Sbjct: 121 IKAPPHESCNFSPTESCRASCELNPDEYLLRFRGE-IKDFVGNPPRKSWSNKVKQLKQSS 180

Query: 181 QAYFKAMFGKSGCSNKSSAKSVFNEETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNS 240
           QAYFKA+FGKS CS+KS    +FNEET E V  +     K + K PFG+IDYNKWK+ ++
Sbjct: 181 QAYFKALFGKSPCSSKS----LFNEET-ECVFKNRVSSWKFINKTPFGRIDYNKWKIQST 240

Query: 241 GLEKEKIEEFYGGILN---NHRRSFSGAIQRNSSATNNKSSSSSSS---SSSSSASSSGS 300
            +EKEK EEF    LN   NHRRSFSGAIQ  SS+ ++ SS SS S   SSSSS SSS S
Sbjct: 241 FIEKEKTEEFSSSSLNMNNNHRRSFSGAIQTASSSCSSCSSCSSCSCSCSSSSSGSSSLS 300

Query: 301 SSFSFSSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQAETCS 360
           SSFSF           KRS S NSEIE SIEGAIAHCK+SQK+  +S+K E ES  ETC 
Sbjct: 301 SSFSF-----------KRSNSANSEIERSIEGAIAHCKQSQKVLDNSRKREAESSPETCG 356

Query: 361 LSASKIFLPCGDQKS 364
           +S SK+ LPCG+QKS
Sbjct: 361 VSVSKVVLPCGNQKS 356

BLAST of MC01g0490 vs. ExPASy TrEMBL
Match: A0A5A7TV49 (Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G001530 PE=4 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 1.17e-133
Identity = 235/331 (71.00%), Postives = 270/331 (81.57%), Query Frame = 0

Query: 52  MTSFSGQTEPATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFP 111
           MTSFSG+TE ATSPAD+LFYKGKLLPLHLPPRLQMVQKLLHSP+SQNPS K +SFSENF 
Sbjct: 1   MTSFSGETEAATSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 60

Query: 112 IPLISIAAPL-ESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFLGDPPR--KSWSKKM 171
           IP IS+ AP  ES + SP+ESCR SCELNPDE+L+G+RGE IK F+       KSWSKK+
Sbjct: 61  IPFISMKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTTTKSWSKKV 120

Query: 172 KQLKNSSQAYFKAMFGKSGCSNKSSAKSVFNEETAEGVL---GSSGQCDKVVKKNPFGKI 231
           KQLK SSQAYFK++FGKS CS+ + + SVFNEE+ E +    GSSG+  KV KKNPFG+I
Sbjct: 121 KQLKQSSQAYFKSLFGKSVCSSNNRS-SVFNEES-ENIFDNRGSSGKFIKVSKKNPFGRI 180

Query: 232 DYNKWKMPNSGLEKEKIEEFYGGILN--NHRRSFSGAIQRNSSAT----NNKSSSSSSSS 291
           DYNKW++PN  LEKEK E+FYGGILN  NHRRSFSGAIQR SS++    NN +  SSS S
Sbjct: 181 DYNKWQIPNQTLEKEKAEDFYGGILNINNHRRSFSGAIQRTSSSSSSSINNNNKISSSCS 240

Query: 292 SSSSASSSGSSSFSFSSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKN 351
           +SSS SSS SSSFSFSSNGF DLQ FKRSIS NSEIE+SIEGAIAHCK+SQK+  +S+KN
Sbjct: 241 TSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKN 300

Query: 352 ERESQAETCSLSASKIFLPCGDQKSSGLCSI 370
           E ESQ +TCSLS SK+ LPCGDQKSS LC I
Sbjct: 301 ETESQPQTCSLSVSKV-LPCGDQKSSQLCRI 328

BLAST of MC01g0490 vs. ExPASy TrEMBL
Match: A0A6J1GG61 (probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN=LOC111453872 PE=4 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 3.53e-133
Identity = 248/376 (65.96%), Postives = 279/376 (74.20%), Query Frame = 0

Query: 1   MATAKASLAAPPPEEDYIDMELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSGQTE 60
           MAT +A     P EEDYIDMELTSS SS  N F YSI SPP +RDFEFQ QMTSFSG+TE
Sbjct: 1   MATTEAPFTTAPAEEDYIDMELTSSPSS--NSFSYSIRSPPPARDFEFQIQMTSFSGETE 60

Query: 61  PATSPADELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAP 120
             TSPAD+LFYKGKLLPLHLPPRLQMVQ LLHSP+S NPS K +SF ENF IP I+I AP
Sbjct: 61  QTTSPADDLFYKGKLLPLHLPPRLQMVQNLLHSPNSHNPSTKNKSFPENFQIPFIAIKAP 120

Query: 121 L-ESGSFSPTESCRASCELNPDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNSSQAYF 180
             ES +FSPTES RASCELNPDEYL+ +RGE IK F+G+PPRKSWS K+KQLK SSQAYF
Sbjct: 121 PHESCNFSPTESSRASCELNPDEYLLRFRGE-IKDFVGNPPRKSWSNKVKQLKQSSQAYF 180

Query: 181 KAMFGKSGCSNKSSAKSVFNEETAEGVLG--SSGQCDKVVKKNPFGKIDYNKWKMPNSGL 240
           KA+FGKS CS KS    +FNEET  G     SSG   K + K PFG+IDYNKWK+  + +
Sbjct: 181 KALFGKSPCSTKS----LFNEETECGFKNRVSSG---KFINKTPFGRIDYNKWKIQTTFI 240

Query: 241 EKEKIEEFYGGILN---NHRRSFSGAIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSF 300
           EKEK EEF G  LN   NHRRSFSGAIQ  SS+ ++ S  SS SS S S SSS S S S 
Sbjct: 241 EKEKTEEFSGSSLNMNNNHRRSFSGAIQTASSSCSSCSLCSSCSSCSCSCSSSSSGSSSL 300

Query: 301 SSNGFSDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQAETCSLSASK 360
           SS+ FS    FKRS S NSEIE SIEGAIAHCK+SQK+  +S+K E ES  ETC +S SK
Sbjct: 301 SSS-FS----FKRSNSANSEIERSIEGAIAHCKQSQKVLDNSRKREAESSPETCCVSVSK 360

Query: 361 IFLPCGDQKSSGLCSI 370
           + LPCG+QKS  LC I
Sbjct: 361 V-LPCGNQKSYELCRI 360

BLAST of MC01g0490 vs. TAIR 10
Match: AT2G39370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G37380.1); Has 184 Blast hits to 178 proteins in 53 species: Archae - 0; Bacteria - 58; Metazoa - 9; Fungi - 0; Plants - 103; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink). )

HSP 1 Score: 160.2 bits (404), Expect = 3.1e-39
Identity = 130/352 (36.93%), Postives = 188/352 (53.41%), Query Frame = 0

Query: 14  EEDYIDMELTSSSSSSSNLFCYSIESPPQSRDFEFQFQMTSFSG-QTEPATSPADELFYK 73
           EEDYIDME+T    S +NL   ++ +   +   EF+FQM+     + +  TSPADELFYK
Sbjct: 12  EEDYIDMEVT----SFTNLVRKTLSN---NYPREFEFQMSHLCPLEIDKTTSPADELFYK 71

Query: 74  GKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFSPTESC 133
           GKLLPLHLPPRLQMVQK+L   +  +        +     P+ S   P ES + SP ESC
Sbjct: 72  GKLLPLHLPPRLQMVQKILEDYTFDDEFYSTPLATGTVTTPVTS-NTPFESCTVSPAESC 131

Query: 134 RASCELNPDEYLVGWRGEEIKGFLGDPPRKSWSKKMKQLKNS--------SQAYFKAMFG 193
           + S ELNP++Y + +     +    D  +KSW+ K++ +K S        S+AY ++ FG
Sbjct: 132 QVSKELNPEDYFLEYSDSLEE---DDEKKKSWTTKLRLMKQSSLGTKIKASRAYLRSFFG 191

Query: 194 KSGCSNKSS-AKSVFNEETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEKEKIE 253
           K+ CS++SS A S       + VL  S       +  PFG+I   + K  ++        
Sbjct: 192 KTSCSDESSCASSAARVADEDSVLRYS-------RVKPFGQIKTERPKKQSN-------- 251

Query: 254 EFYGGILNNHRRSFSGAIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGFSDLQ 313
              G +  +HRRSFS +++R ++ ++N  SS+S                     GF  LQ
Sbjct: 252 ---GSVSGSHRRSFSVSMRRQAAKSSNNKSSNSL--------------------GFRPLQ 311

Query: 314 LFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQAETCSLSASKI 356
             KRS S +SEIE+SI+GAI HCK+SQ+     K+    ++   CSLSAS+I
Sbjct: 312 FLKRSTSSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRI 314

BLAST of MC01g0490 vs. TAIR 10
Match: AT2G37380.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G39370.1); Has 1284 Blast hits to 422 proteins in 114 species: Archae - 0; Bacteria - 90; Metazoa - 125; Fungi - 151; Plants - 136; Viruses - 0; Other Eukaryotes - 782 (source: NCBI BLink). )

HSP 1 Score: 148.7 bits (374), Expect = 9.4e-36
Identity = 149/362 (41.16%), Postives = 186/362 (51.38%), Query Frame = 0

Query: 14  EEDYIDMEL-----TSSSSSSSNLFCYSI-ESPPQSRDFEFQFQMTSFSGQTEPATSPAD 73
           ++ YIDME+     +SSS+SSS+ F + +  SPPQSR+FEFQ   +S     E  TSPAD
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQ-MCSSAVASGESTTSPAD 77

Query: 74  ELFYKGKLLPLHLPPRLQMVQKLLHSPSSQNPSIKEQSFSENFPIPLISIAAPLESGSFS 133
           ELFYKG+LLPLHLPPRL+MVQKLL + SS   + +        PI   + A  L    FS
Sbjct: 78  ELFYKGQLLPLHLPPRLKMVQKLLLASSSSTAATET-------PISPRAAADVLSPRRFS 137

Query: 134 PTESCRASCELNPDEYLVGWRGEEIKGFLGDPPR---KSWSKKMK-----QLKNSSQAYF 193
                  SCE+  DE        E+K F+         SWSKK+K     Q   +S+AY 
Sbjct: 138 -------SCEIGQDENCFFEISTELKRFIESNENHLGNSWSKKIKHSSITQKLKASRAYI 197

Query: 194 KAMFGKSGCSNKSSAKSVFNEETAEGVLGSSGQCDKVVKKNPFGKIDYNKWKMPNSGLEK 253
           KA+F K  CS+ S     F  E ++             KKNPF   +             
Sbjct: 198 KALFSKQACSDSSEINPRFKIEPSK----------VSRKKNPFVNSE------------- 257

Query: 254 EKIEEFYGGILNNHRRSFSGAIQRNSSATNNKSSSSSSSSSSSSASSSGSSSFSFSSNGF 313
                     L  HRRSFSG IQR+S A        S+SSSSSS++SS SSSFSF SNG 
Sbjct: 258 --------NPLLIHRRSFSGVIQRHSQA------KCSTSSSSSSSASSLSSSFSFGSNGS 317

Query: 314 SDLQLFKRSISENSEIESSIEGAIAHCKESQKLFGSSKKNERESQAETCSLSASKIFLPC 362
            DLQ   RS   ++  ++SIEGAI HCK+S   F + K N  ES  E CS   S     C
Sbjct: 318 LDLQTLMRS---SNASDNSIEGAIEHCKQS---FTTRKSNVTES--ELCSSRTS--VSTC 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O806244.4e-3836.93Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9ZUS81.3e-3441.16Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_022143838.19.55e-237100.00probable membrane-associated kinase regulator 3 [Momordica charantia][more]
XP_038883185.14.20e-15571.11probable membrane-associated kinase regulator 4 [Benincasa hispida][more]
KGN54211.11.34e-15369.92hypothetical protein Csa_018049 [Cucumis sativus][more]
XP_004142216.21.69e-13562.08probable membrane-associated kinase regulator 4 [Cucumis sativus][more]
XP_022978386.19.59e-13465.60probable membrane-associated kinase regulator 4 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1CRQ34.62e-237100.00probable membrane-associated kinase regulator 3 OS=Momordica charantia OX=3673 G... [more]
A0A0A0L2346.49e-15469.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G293210 PE=4 SV=1[more]
A0A6J1IMI84.64e-13465.60probable membrane-associated kinase regulator 4 isoform X1 OS=Cucurbita maxima O... [more]
A0A5A7TV491.17e-13371.00Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1... [more]
A0A6J1GG613.53e-13365.96probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT2G39370.13.1e-3936.93unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT2G37380.19.4e-3641.16unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 262..287
NoneNo IPR availablePANTHERPTHR33312:SF21MEMBRANE-ASSOCIATED KINASE REGULATOR 3-RELATEDcoord: 1..370
IPR039620BKI1/Probable membrane-associated kinase regulator 1/3/4PANTHERPTHR33312MEMBRANE-ASSOCIATED KINASE REGULATOR 4-RELATEDcoord: 1..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g0490.1MC01g0490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity
molecular_function GO:0019210 kinase inhibitor activity