MC01g0280 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0280
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	chaperone protein dnaJ 72
Location	MC01: 9446983 .. 9450884 (-)
RNA-Seq Expression	MC01g0280
Synteny	MC01g0280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCGGGACTCGAGAGAAATGGGCTTAATGGGCCGAAAAATCAACGCGCGGCCCAATACGCTTCATTCTTGAATTACTTGGAAGAAAGGCAAGAGAAGTGGCCATGGCGGTGCCACGAGGTCCGAGATCCTCCGGGTCTCCTTTTCCGCCGCGAACCCGAATTTCTTCCAGAAGCTGGAGCTCCGATTCGAATTCCATGGAAGCCCCTCGATTCCAGGCTCTCGTGAAGAAGCGATCGCCGCCGCCGTCGTCGTCTACTCCATTTCTCGAAGGTTTCATCTCCCGCCGCCGCTGCAATTGATCGGAGAGACCCATCCGCCATGGATGCTCGCGATCACTACAAGGTGCTGGGCTTGAACAGACGCGCCACGAAGGAAGAAATCAAGGAGGCATTCAGGAAGCTGGCGAAGGAATTTCACCCTGATAAGCATTCCCAATCGCCCAAGGCCATCAGGGACTCTGCTACTTTGAGGTTCAAGCAAGTTTCGGAAGCATATGAAATCCTCGGCGATGATTGTAAGCGGGCTGATTACAATATTCGCTCTCGTTGCGCCTCTGGTCCTTCTGTTAATCATCATTATTACTCGTCGTCGTATAATTCTTATGCTAATGCGAGTGGACCTCGATATGCTTCTTCTTCGGGATTTGCCTCTCGCTCTGGTTCCAATGTTGAAGGATTTGTTACGAATGTTCATATGCTTCTACGATTTCTCACCACGCGGGCTTTTCTTCTCAATTTTGCTTTTGCCGGGTATTTATACTTTACCAATACAATTTATGCTTTGAATTTTGGTAGTAAAGTGCTTCTTCGTTCCAACTTCATGGTTAACAACATCAACTCTCTCTCAGGACAAAAGCTTCCGCTTCCCATATCTAAACTCATTTTCAATTTAAGATTGTATTGACCCAGATTCTGTGTTCTTTCATTGTGCTCCTCCATCTTCACGGTCTTCACCCCAAAATTTGTTTCCTGATTCTCCACATAAGTTACAGTGTTTGTTAGTTTTCTGCATTATGATTTAGCATGTATTTGCTCGATGATGCTGCTGTGATGTAAGTGAAAAGTTGCACTACAAAACCCAATACGTGCTGCAACTCATGTTCAATCCTGTTAAATGATTTTTTCGTATCCTCAAAGTCATATTCGTTTGCGGTCAGAATTCTCCTTCAGATGTTTTTGTATCCTTCGGGTACGGTGAGCATGAACATCACTAAGAGTGATTTTTGGGAGAAACACCAATCGACTGCTTTTAGAATCACACTTGACTATATAACCATATAGCCAAATGTTATGAAAATTTTGAAAGTGCTTTTAATGGGCTAAAAGCACTTTCTACTGTTCCAAAATCATACCAAACTCTCCATATGGCAAGGCTGCTGCTCATGGAAGATAAAGTTTTTCTTATGGGAAGTATCTCGTGGAGCTATTAACTCTCTCACGAAGTTGGTACCAAATTTGCCGGTTGAACAATGAATCCCAAATTCCAATCCCATATTTTTGTTCTCTGCCCCTATGCCCAAAGTTTCTGGAATTTTGCAAGAGTTTCAATTGGCACACAGCCTTTCCAGTGCCTATGTCTACTAGCCCCTATGATTCGAATACACATCAATTCTCGGACCCTGCTGTTATCCGCTACATTCAAAAGCTTTTATATTCTACATCCTCATAGGGCACCCCTTTAAAGAGGAAAAAGCAAAGCAAACCTTATGGATGGACATAGTCATAGTCGGAGCATTCTTTTGGAAGATTTGGTTAGAGAGGAATGATTGTGCCTTAAATGGTTCTAAAGATCCTTTAAGCTCCTTTGTGAATTCCATTATGGCTCTTCATTGGTGTAAATGCAAGCAACCCTTCTCATTATAGTTTAACTAGGAATTACCTTTTTTGTAACTCCATTTTTGGGCTCTTGGGTGAACCCTTCTTTTTGTAATATTTTATCAATTAATGAAATACTTGTTTCCTAAAAAAAAAAGATACCAAACCGACACCTAAATAGTTGGTGTCTTAGCTAGACCTTGACAAGATGTGAATATTCTTGGCTAGGCCCTTGATTTCCACGAAGATTTCATTTATGGGAACGGGTTTGTTGTTCCTGGTCCGAGATAAGGTTGACCATAGCAGTTCTTACTGCCGTTTGAATCTGAAAGTTGCTCCAGTAGAAACTTGGGAATGGAAAAAGGTTTACTGCTAACTTGAGAGAAAAGGGACTGAAAAGTAAACAAAATAAATCAAGGAAACCAAAATTTTAGTCTATTATGGATCCATGGCCAAATATTATTGGTGCTAAGCATACCTGGCTATTGGCCCAAGAATTGACTGTTCTATTTTTTTAGCTTATAGTTTCTGTTTAGCTGTTGATGATCATTTCATAATAGAGTTGTTGACATGTATTTTGTTGTTTGACTTGGATATTCTAGGTGAAATTGAGAATAATCTACGAGTTCGAGTATATTTTTAGCCTGTTTCCACTTCCATAATTCTCAATCAGTTTTTACAAGGTCTGTGTTTGCAGAGCACGGGCTCTTTGACCGCAAATAGTTGTCATTGTTGCATTTTTGTGCAGTGCTTTAGTTGGTGGCATGTTTGCGATTGACACGAGTGGGGAGGCCTTGTGGAAGATGCACAATTCTGGGGTATGTAACTATTTGTTCTCTTATCCTCCTCCCTCTCACCCTATTAGTCCCTAGCAAAACACATTATTAATAACGGAAAAACATCCTTGTATTTTTCAAGCTTTTGCAAATCTTACCTGTATTTTGTGAACAGTTTACTTTCATACTTATTTCGGTTTGGTCAATTGTGAAATGTTATATTTTAGTCCAAATCGTTGATGTTTCATTAATCACTCGATAAGAATGATCTATTTAAAATTCAACATCTCCTAACCTACTAGTACTACTAAGGTTGTGTTTGTAGAGTCAGTGATTTTGATAAAAGAGATTTAGGTATAATTGATTTTCCAAAATCATCGTCTAAGTGATACCTTAAAAATCAATTTAAAGTGATTTTAAACTTTCTTAAAATCAATTTTTTTATTTGCGAAACATGATCAATGAAAATGGAAGTGATTTTGGTCATGCCACATGCCAAAATCACTCCCAAACACACCCTAAATGGTTAGAAAATGATTTTAATGGAAAGTTCATGGACTAGGAACAAAATATTTGGAGTTTAATAGATTAAAATAGAATGAGCATCAAAGTTCATAGACCATAATAGGATTTAGGCATCTAAACTTTTTTCCTTCTATCTAGCACATCTGAAGTAAGTGATGTATGTCCATTCCATTAGAAATAACGGTGAAATTTAACATAATATGTGGTCTGTAGATTTTCAGACCATATACACGTCCAAATTTATATTCAAGACAGAAAGTGTATGATGTATAGATTTTACAACCCATGGAATGACTATTGCAATCTACTCTTAACAATTTTGGGCAGAATTTTACCTGCTCACTTGTTATATTTGTAATTTTTCAATTTTGAAATGGTTAGAGATCGGAGAAAACTACGAAATCTTTTGTATTTTATATATACTTTTTGGATTACGGGGGCGTCCCCTCCGCCTTTGATTATACTTAAGATTATGACTGGTTTTCCTCTCAGAAATCGTTTGAAGAAGCCATGGAGTCAATTAATAAAGCCAAAACACATGAAGATGCTTAAAAGTGCTTGCATTAGAATTCATTTCCCACCACCAGATTGTGTTTTTCCAAGTCTTCTTCCTGTGGGGTTAACATTCCACATGTAAGTGAAGTTGAGACTCACTTGACCATACAAAGAGAACTGGAGAACTGTATGAAATGTATATCTATATATTCTATCTTTTTCTCATCTCCTTGCTGCTTTTGTGTGAATATATGGATATAGATTGTAGGAAAATTTTGGAATTGTTGTCTG

mRNA sequence

GCCGGGACTCGAGAGAAATGGGCTTAATGGGCCGAAAAATCAACGCGCGGCCCAATACGCTTCATTCTTGAATTACTTGGAAGAAAGGCAAGAGAAGTGGCCATGGCGGTGCCACGAGGTCCGAGATCCTCCGGGTCTCCTTTTCCGCCGCGAACCCGAATTTCTTCCAGAAGCTGGAGCTCCGATTCGAATTCCATGGAAGCCCCTCGATTCCAGGCTCTCGTGAAGAAGCGATCGCCGCCGCCGTCGTCGTCTACTCCATTTCTCGAAGGTTTCATCTCCCGCCGCCGCTGCAATTGATCGGAGAGACCCATCCGCCATGGATGCTCGCGATCACTACAAGGTGCTGGGCTTGAACAGACGCGCCACGAAGGAAGAAATCAAGGAGGCATTCAGGAAGCTGGCGAAGGAATTTCACCCTGATAAGCATTCCCAATCGCCCAAGGCCATCAGGGACTCTGCTACTTTGAGGTTCAAGCAAGTTTCGGAAGCATATGAAATCCTCGGCGATGATTGTAAGCGGGCTGATTACAATATTCGCTCTCGTTGCGCCTCTGGTCCTTCTGTTAATCATCATTATTACTCGTCGTCGTATAATTCTTATGCTAATGCGAGTGGACCTCGATATGCTTCTTCTTCGGGATTTGCCTCTCGCTCTGGTTCCAATGTTGAAGGATTTGTTACGAATGTTCATATGCTTCTACGATTTCTCACCACGCGGGCTTTTCTTCTCAATTTTGCTTTTGCCGGTGCTTTAGTTGGTGGCATGTTTGCGATTGACACGAGTGGGGAGGCCTTGTGGAAGATGCACAATTCTGGGAAATCGTTTGAAGAAGCCATGGAGTCAATTAATAAAGCCAAAACACATGAAGATGCTTAAAAGTGCTTGCATTAGAATTCATTTCCCACCACCAGATTGTGTTTTTCCAAGTCTTCTTCCTGTGGGGTTAACATTCCACATGTAAGTGAAGTTGAGACTCACTTGACCATACAAAGAGAACTGGAGAACTGTATGAAATGTATATCTATATATTCTATCTTTTTCTCATCTCCTTGCTGCTTTTGTGTGAATATATGGATATAGATTGTAGGAAAATTTTGGAATTGTTGTCTG

Coding sequence (CDS)

ATGGATGCTCGCGATCACTACAAGGTGCTGGGCTTGAACAGACGCGCCACGAAGGAAGAAATCAAGGAGGCATTCAGGAAGCTGGCGAAGGAATTTCACCCTGATAAGCATTCCCAATCGCCCAAGGCCATCAGGGACTCTGCTACTTTGAGGTTCAAGCAAGTTTCGGAAGCATATGAAATCCTCGGCGATGATTGTAAGCGGGCTGATTACAATATTCGCTCTCGTTGCGCCTCTGGTCCTTCTGTTAATCATCATTATTACTCGTCGTCGTATAATTCTTATGCTAATGCGAGTGGACCTCGATATGCTTCTTCTTCGGGATTTGCCTCTCGCTCTGGTTCCAATGTTGAAGGATTTGTTACGAATGTTCATATGCTTCTACGATTTCTCACCACGCGGGCTTTTCTTCTCAATTTTGCTTTTGCCGGTGCTTTAGTTGGTGGCATGTTTGCGATTGACACGAGTGGGGAGGCCTTGTGGAAGATGCACAATTCTGGGAAATCGTTTGAAGAAGCCATGGAGTCAATTAATAAAGCCAAAACACATGAAGATGCTTAA

Protein sequence

MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYEILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGFVTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKAKTHEDA

Homology

[Close]

BLAST of MC01g0280 vs. ExPASy Swiss-Prot
Match: Q0WTI8 (Chaperone protein dnaJ 72 OS=Arabidopsis thaliana OX=3702 GN=ATJ72 PE=2 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.5e-42
Identity = 96/181 (53.04%), Postives = 121/181 (66.85%), Query Frame = 0


Query: 5   DHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYEILGD 64
           DHY+VLG+ R ATK+E+K+AFR+LA ++HPDKH+QSP+ +R +AT+RFK VSEAYE+L D
Sbjct: 3   DHYQVLGVTRNATKKEVKDAFRRLAIKYHPDKHAQSPEHVRHNATVRFKLVSEAYEVLND 62

Query: 65  DCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGFVTNV 124
           D KRA YN  S        +  Y +   N    A G  Y    G+++R+      F +  
Sbjct: 63  DLKRASYNAGSDSDCFRRTSGSYSNPYGNRGGRAQGSGYGYGYGYSTRN-RQASSFSSGF 122

Query: 125 HMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKAKTHE 184
               R+LTTRAFLLN A AG L     AIDTSGE LWKM NSGKSFEEAMESI K+K+H+
Sbjct: 123 DSTFRYLTTRAFLLNLALAGGLYFAFTAIDTSGETLWKMRNSGKSFEEAMESIEKSKSHK 182

Query: 185 D 186
           D
Sbjct: 183 D 182

[Close]

BLAST of MC01g0280 vs. ExPASy Swiss-Prot
Match: Q1H3B9 (Chaperone protein DnaJ OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) OX=265072 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.2e-13
Identity = 45/108 (41.67%), Postives = 64/108 (59.26%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           M  RD+Y+VLG+NR AT EEIK+A+RKLA ++HPD++  +PKA        FK+  EAYE
Sbjct: 1   MAKRDYYEVLGVNRDATDEEIKKAYRKLAMKYHPDRNPDNPKAEE-----HFKEAKEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSG 109
           +L DD KRA Y+        PS      +  + ++A+A G  +    G
Sbjct: 61  VLSDDQKRAAYDQYGHAGVDPSAAAGAGAGGFGNFADAFGDIFGDIFG 103

[Close]

BLAST of MC01g0280 vs. ExPASy Swiss-Prot
Match: Q8CP18 (Chaperone protein DnaJ OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.4e-13
Identity = 35/72 (48.61%), Postives = 56/72 (77.78%), Query Frame = 0


Query: 1  MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
          M  RD+Y+VLG+N+ A+K+EIK+A+RKL+K++HPD + +      + A  +FK++SEAYE
Sbjct: 1  MAKRDYYEVLGVNKSASKDEIKKAYRKLSKKYHPDINKE------EGADEKFKEISEAYE 60

Query: 61 ILGDDCKRADYN 73
          +L D+ KRA+Y+
Sbjct: 61 VLSDENKRANYD 66

[Close]

BLAST of MC01g0280 vs. ExPASy Swiss-Prot
Match: C3P8L9 (Chaperone protein DnaJ OS=Bacillus anthracis (strain A0248) OX=592021 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = 0


Query: 1  MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
          M  RD+Y+VLGL++ A+K+EIK+A+R+LAK++HPD   +      ++A  +FK+V EAYE
Sbjct: 1  MSKRDYYEVLGLSKGASKDEIKKAYRRLAKKYHPDVSKE------ENAIEKFKEVQEAYE 60

Query: 61 ILGDDCKRADYN 73
          +L DD KRA Y+
Sbjct: 61 VLSDDQKRAQYD 66

[Close]

BLAST of MC01g0280 vs. ExPASy Swiss-Prot
Match: C3L5R6 (Chaperone protein DnaJ OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) OX=568206 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = 0


Query: 1  MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
          M  RD+Y+VLGL++ A+K+EIK+A+R+LAK++HPD   +      ++A  +FK+V EAYE
Sbjct: 1  MSKRDYYEVLGLSKGASKDEIKKAYRRLAKKYHPDVSKE------ENAIEKFKEVQEAYE 60

Query: 61 ILGDDCKRADYN 73
          +L DD KRA Y+
Sbjct: 61 VLSDDQKRAQYD 66

[Close]

BLAST of MC01g0280 vs. NCBI nr
Match: XP_022154540.1 (chaperone protein dnaJ 72 [Momordica charantia])

HSP 1 Score: 360 bits (925), Expect = 2.26e-125
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE
Sbjct: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGF 120
           ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGF
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGF 120

Query: 121 VTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKA 180
           VTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKA
Sbjct: 121 VTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKA 180

Query: 181 KTHEDA 186
           KTHEDA
Sbjct: 181 KTHEDA 186

[Close]

BLAST of MC01g0280 vs. NCBI nr
Match: KAG6595464.1 (Chaperone protein dnaJ 72, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 328 bits (841), Expect = 1.50e-112
Identity = 172/188 (91.49%), Postives = 176/188 (93.62%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MD RDHYKVLGLNR ATKEE+KEAFRKLAKEFHPDKHSQSPK++RDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYA--SSSGFASRSGSNVE 120
           ILGDDCKRADYNIRSRCASGPSVN HYYSS YNSYANASGPRY   SSSGFASRSGSNV+
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSS-YNSYANASGPRYGYGSSSGFASRSGSNVD 120

Query: 121 GFVTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180
           G  TN HMLLRFLTTRAFLLN AFAG LVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN
Sbjct: 121 GLFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180

Query: 181 KAKTHEDA 186
           KAKTHEDA
Sbjct: 181 KAKTHEDA 187

[Close]

BLAST of MC01g0280 vs. NCBI nr
Match: XP_022925035.1 (chaperone protein dnaJ 72 [Cucurbita moschata])

HSP 1 Score: 327 bits (837), Expect = 6.09e-112
Identity = 171/188 (90.96%), Postives = 175/188 (93.09%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MD RDHYKVLGLNR ATKEE+KEAFRKLAKEFHPDKHSQSPK++RDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYA--SSSGFASRSGSNVE 120
           ILGDDCKRADYNIRSRCASGPSVN HYYSS YNSYAN SGPRY   SSSGFASRSGSNV+
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSS-YNSYANGSGPRYGYGSSSGFASRSGSNVD 120

Query: 121 GFVTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180
           G  TN HMLLRFLTTRAFLLN AFAG LVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN
Sbjct: 121 GLFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180

Query: 181 KAKTHEDA 186
           KAKTHEDA
Sbjct: 181 KAKTHEDA 187

[Close]

BLAST of MC01g0280 vs. NCBI nr
Match: KAG7027464.1 (Chaperone protein dnaJ 72 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 325 bits (833), Expect = 2.48e-111
Identity = 171/188 (90.96%), Postives = 175/188 (93.09%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MD RDHYKVLGLNR ATKEE+KEAFRKLAKEFHPDKHSQSPK++RDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYA--SSSGFASRSGSNVE 120
           ILGDDCKRADYNIRSRCASGPSVN HYYSS YNSYANASGPRY   SSSGFASRSGSNV+
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSS-YNSYANASGPRYGYGSSSGFASRSGSNVD 120

Query: 121 GFVTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180
           G  TN HMLLRFLTTRAFLLN AFAG LVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN
Sbjct: 121 GLFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180

Query: 181 KAKTHEDA 186
           KAKT EDA
Sbjct: 181 KAKTQEDA 187

[Close]

BLAST of MC01g0280 vs. NCBI nr
Match: XP_023518661.1 (chaperone protein dnaJ 72 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 323 bits (827), Expect = 2.04e-110
Identity = 170/188 (90.43%), Postives = 174/188 (92.55%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MD RDHYKVLGLNR ATKEE+KEAFRKLAKEFHPDKHSQSPKA+RDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKAVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYA--SSSGFASRSGSNVE 120
           ILGDDCKRADYNIRSRCASGPSV  HYYSS YNSYANASGPRY   SSSGFA+RSGSNV+
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVKQHYYSS-YNSYANASGPRYGYGSSSGFAARSGSNVD 120

Query: 121 GFVTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180
           G  TN HMLLRFLTTRAFLLN AFAG LVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN
Sbjct: 121 GLFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180

Query: 181 KAKTHEDA 186
           KAKT EDA
Sbjct: 181 KAKTQEDA 187

[Close]

BLAST of MC01g0280 vs. ExPASy TrEMBL
Match: A0A6J1DJW0 (chaperone protein dnaJ 72 OS=Momordica charantia OX=3673 GN=LOC111021790 PE=4 SV=1)

HSP 1 Score: 360 bits (925), Expect = 1.09e-125
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE
Sbjct: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGF 120
           ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGF
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGF 120

Query: 121 VTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKA 180
           VTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKA
Sbjct: 121 VTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKA 180

Query: 181 KTHEDA 186
           KTHEDA
Sbjct: 181 KTHEDA 186

[Close]

BLAST of MC01g0280 vs. ExPASy TrEMBL
Match: A0A6J1EGR8 (chaperone protein dnaJ 72 OS=Cucurbita moschata OX=3662 GN=LOC111432401 PE=4 SV=1)

HSP 1 Score: 327 bits (837), Expect = 2.95e-112
Identity = 171/188 (90.96%), Postives = 175/188 (93.09%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MD RDHYKVLGLNR ATKEE+KEAFRKLAKEFHPDKHSQSPK++RDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKSVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYA--SSSGFASRSGSNVE 120
           ILGDDCKRADYNIRSRCASGPSVN HYYSS YNSYAN SGPRY   SSSGFASRSGSNV+
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQHYYSS-YNSYANGSGPRYGYGSSSGFASRSGSNVD 120

Query: 121 GFVTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180
           G  TN HMLLRFLTTRAFLLN AFAG LVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN
Sbjct: 121 GLFTNFHMLLRFLTTRAFLLNLAFAGVLVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180

Query: 181 KAKTHEDA 186
           KAKTHEDA
Sbjct: 181 KAKTHEDA 187

[Close]

BLAST of MC01g0280 vs. ExPASy TrEMBL
Match: A0A6J1HRQ1 (chaperone protein dnaJ 72 OS=Cucurbita maxima OX=3661 GN=LOC111466112 PE=4 SV=1)

HSP 1 Score: 318 bits (815), Expect = 6.65e-109
Identity = 168/188 (89.36%), Postives = 173/188 (92.02%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MD RDHYKVLGLNR ATKEE+KEAFRKLAKEFHPDKHSQSPKA+RDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLNRSATKEEVKEAFRKLAKEFHPDKHSQSPKAVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYA--SSSGFASRSGSNVE 120
           ILGDDCKRADYNIRSRCASGP VN HYYSS YNSYANASGPRY   SSSGFASRSGSNV+
Sbjct: 61  ILGDDCKRADYNIRSRCASGPYVNQHYYSS-YNSYANASGPRYGYGSSSGFASRSGSNVD 120

Query: 121 GFVTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESIN 180
           G  TN HMLLRFLTTRAFLLN AFAG LVGG+FAIDTSGEALWKMHNSGKSFEEAM SI+
Sbjct: 121 GLFTNFHMLLRFLTTRAFLLNLAFAGVLVGGIFAIDTSGEALWKMHNSGKSFEEAMASID 180

Query: 181 KAKTHEDA 186
           KAKT EDA
Sbjct: 181 KAKTQEDA 187

[Close]

BLAST of MC01g0280 vs. ExPASy TrEMBL
Match: A0A1S3B4T7 (chaperone protein dnaJ 72 OS=Cucumis melo OX=3656 GN=LOC103486026 PE=4 SV=1)

HSP 1 Score: 315 bits (807), Expect = 1.03e-107
Identity = 164/186 (88.17%), Postives = 169/186 (90.86%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MD RDHYKVLGL R ATKEEIK+AFRKLAKEFHPDKHSQSPK +RDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLTRSATKEEIKDAFRKLAKEFHPDKHSQSPKVVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGF 120
           ILGDDCKRADYNIRSRCASGPSVN  YYSS YNSYA ASGPRY SSSGFASRS  N +G 
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQQYYSS-YNSYARASGPRYGSSSGFASRSRFNADGL 120

Query: 121 VTNVHMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKA 180
           VTN HMLLRFLTTRAFLLNFAFAG L GGM AID+SGEALWKMHNSGKSFEEAM+SINKA
Sbjct: 121 VTNFHMLLRFLTTRAFLLNFAFAGVLAGGMLAIDSSGEALWKMHNSGKSFEEAMDSINKA 180

Query: 181 KTHEDA 186
           KTHEDA
Sbjct: 181 KTHEDA 185

[Close]

BLAST of MC01g0280 vs. ExPASy TrEMBL
Match: A0A5A7TEJ7 (Chaperone protein dnaJ 72 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G00350 PE=4 SV=1)

HSP 1 Score: 243 bits (621), Expect = 1.51e-79
Identity = 128/150 (85.33%), Postives = 132/150 (88.00%), Query Frame = 0


Query: 1   MDARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYE 60
           MD RDHYKVLGL R ATKEEIK+AFRKLAKEFHPDKHSQSPK +RDSATLRFKQVSEAYE
Sbjct: 1   MDVRDHYKVLGLTRSATKEEIKDAFRKLAKEFHPDKHSQSPKVVRDSATLRFKQVSEAYE 60

Query: 61  ILGDDCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGF 120
           ILGDDCKRADYNIRSRCASGPSVN  YYSS YNSYA ASGPRY SSSGFASRS  N +G 
Sbjct: 61  ILGDDCKRADYNIRSRCASGPSVNQQYYSS-YNSYARASGPRYGSSSGFASRSRFNADGL 120

Query: 121 VTNVHMLLRFLTTRAFLLNFAFAGALVGGM 150
           VTN HMLLRFLTTRAFLLNFAFAG L   +
Sbjct: 121 VTNFHMLLRFLTTRAFLLNFAFAGYLFSSL 149

[Close]

BLAST of MC01g0280 vs. TAIR 10
Match: AT2G41000.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 173.3 bits (438), Expect = 1.8e-43
Identity = 96/181 (53.04%), Postives = 121/181 (66.85%), Query Frame = 0


Query: 5   DHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYEILGD 64
           DHY+VLG+ R ATK+E+K+AFR+LA ++HPDKH+QSP+ +R +AT+RFK VSEAYE+L D
Sbjct: 3   DHYQVLGVTRNATKKEVKDAFRRLAIKYHPDKHAQSPEHVRHNATVRFKLVSEAYEVLND 62

Query: 65  DCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGFVTNV 124
           D KRA YN  S        +  Y +   N    A G  Y    G+++R+      F +  
Sbjct: 63  DLKRASYNAGSDSDCFRRTSGSYSNPYGNRGGRAQGSGYGYGYGYSTRN-RQASSFSSGF 122

Query: 125 HMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKSFEEAMESINKAKTHE 184
               R+LTTRAFLLN A AG L     AIDTSGE LWKM NSGKSFEEAMESI K+K+H+
Sbjct: 123 DSTFRYLTTRAFLLNLALAGGLYFAFTAIDTSGETLWKMRNSGKSFEEAMESIEKSKSHK 182

Query: 185 D 186
           D
Sbjct: 183 D 182

[Close]

BLAST of MC01g0280 vs. TAIR 10
Match: AT2G41000.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 1.4e-35
Identity = 83/165 (50.30%), Postives = 105/165 (63.64%), Query Frame = 0


Query: 5   DHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYEILGD 64
           DHY+VLG+ R ATK+E+K+AFR+LA ++HPDKH+QSP+ +R +AT+RFK VSEAYE+L D
Sbjct: 3   DHYQVLGVTRNATKKEVKDAFRRLAIKYHPDKHAQSPEHVRHNATVRFKLVSEAYEVLND 62

Query: 65  DCKRADYNIRSRCASGPSVNHHYYSSSYNSYANASGPRYASSSGFASRSGSNVEGFVTNV 124
           D KRA YN  S        +  Y +   N    A G  Y    G+++R+      F +  
Sbjct: 63  DLKRASYNAGSDSDCFRRTSGSYSNPYGNRGGRAQGSGYGYGYGYSTRN-RQASSFSSGF 122

Query: 125 HMLLRFLTTRAFLLNFAFAGALVGGMFAIDTSGEALWKMHNSGKS 170
               R+LTTRAFLLN A AG L     AIDTSGE LWKM NSG S
Sbjct: 123 DSTFRYLTTRAFLLNLALAGGLYFAFTAIDTSGETLWKMRNSGVS 166

[Close]

BLAST of MC01g0280 vs. TAIR 10
Match: AT1G59725.1 (DNAJ heat shock family protein )

HSP 1 Score: 68.9 bits (167), Expect = 4.8e-12
Identity = 37/97 (38.14%), Postives = 63/97 (64.95%), Query Frame = 0


Query: 5  DHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYEILGD 64
          D+Y VL +N  AT++++K+++R+LA ++HPDK   +P +I+  A  +FKQ+SEAY++L D
Sbjct: 4  DYYNVLNVNPSATEDDLKKSYRRLAMKWHPDK---NPTSIKQEAEAKFKQISEAYDVLSD 63

Query: 65 DCKRADYN------IRSRCASGPSVNHHYYSSSYNSY 96
            KR  Y+      + +  A+  S  H+Y S + N+Y
Sbjct: 64 PNKRQIYDQYGEDGLTATEATASSQQHNYSSGNNNNY 97

[Close]

BLAST of MC01g0280 vs. TAIR 10
Match: AT1G28210.1 (DNAJ heat shock family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-11
Identity = 32/70 (45.71%), Postives = 52/70 (74.29%), Query Frame = 0


Query: 3   ARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYEIL 62
           AR++Y VLG++ +AT+EEIK++F +LAK+FHPD +  +P A R     +F+++ EAYE L
Sbjct: 46  ARNYYDVLGVSPKATREEIKKSFHELAKKFHPDTNRNNPSAKR-----KFQEIREAYETL 105

Query: 63  GDDCKRADYN 73
           G+  +R +Y+
Sbjct: 106 GNSERREEYD 110

[Close]

BLAST of MC01g0280 vs. TAIR 10
Match: AT1G28210.2 (DNAJ heat shock family protein )

HSP 1 Score: 66.6 bits (161), Expect = 2.4e-11
Identity = 32/70 (45.71%), Postives = 52/70 (74.29%), Query Frame = 0


Query: 3   ARDHYKVLGLNRRATKEEIKEAFRKLAKEFHPDKHSQSPKAIRDSATLRFKQVSEAYEIL 62
           AR++Y VLG++ +AT+EEIK++F +LAK+FHPD +  +P A R     +F+++ EAYE L
Sbjct: 46  ARNYYDVLGVSPKATREEIKKSFHELAKKFHPDTNRNNPSAKR-----KFQEIREAYETL 105

Query: 63  GDDCKRADYN 73
           G+  +R +Y+
Sbjct: 106 GNSERREEYD 110

The following BLAST results are available for this feature:

BLAST of MC01g0280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WTI8	2.5e-42	53.04	Chaperone protein dnaJ 72 OS=Arabidopsis thaliana OX=3702 GN=ATJ72 PE=2 SV=1	[more]
Q1H3B9	4.2e-13	41.67	Chaperone protein DnaJ OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / ...	[more]
Q8CP18	9.4e-13	48.61	Chaperone protein DnaJ OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PC...	[more]
C3P8L9	1.2e-12	50.00	Chaperone protein DnaJ OS=Bacillus anthracis (strain A0248) OX=592021 GN=dnaJ PE...	[more]
C3L5R6	1.2e-12	50.00	Chaperone protein DnaJ OS=Bacillus anthracis (strain CDC 684 / NRRL 3495) OX=568...	[more]

BLAST of MC01g0280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154540.1	2.26e-125	100.00	chaperone protein dnaJ 72 [Momordica charantia]	[more]
KAG6595464.1	1.50e-112	91.49	Chaperone protein dnaJ 72, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022925035.1	6.09e-112	90.96	chaperone protein dnaJ 72 [Cucurbita moschata]	[more]
KAG7027464.1	2.48e-111	90.96	Chaperone protein dnaJ 72 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023518661.1	2.04e-110	90.43	chaperone protein dnaJ 72 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC01g0280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJW0	1.09e-125	100.00	chaperone protein dnaJ 72 OS=Momordica charantia OX=3673 GN=LOC111021790 PE=4 SV...	[more]
A0A6J1EGR8	2.95e-112	90.96	chaperone protein dnaJ 72 OS=Cucurbita moschata OX=3662 GN=LOC111432401 PE=4 SV=...	[more]
A0A6J1HRQ1	6.65e-109	89.36	chaperone protein dnaJ 72 OS=Cucurbita maxima OX=3661 GN=LOC111466112 PE=4 SV=1	[more]
A0A1S3B4T7	1.03e-107	88.17	chaperone protein dnaJ 72 OS=Cucumis melo OX=3656 GN=LOC103486026 PE=4 SV=1	[more]
A0A5A7TEJ7	1.51e-79	85.33	Chaperone protein dnaJ 72 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of MC01g0280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G41000.1	1.8e-43	53.04	Chaperone DnaJ-domain superfamily protein	[more]
AT2G41000.2	1.4e-35	50.30	Chaperone DnaJ-domain superfamily protein	[more]
AT1G59725.1	4.8e-12	38.14	DNAJ heat shock family protein	[more]
AT1G28210.1	2.4e-11	45.71	DNAJ heat shock family protein	[more]
AT1G28210.2	2.4e-11	45.71	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 47..67 score: 46.75 coord: 25..40 score: 44.16 coord: 7..25 score: 45.85
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 4..67 e-value: 1.5E-24 score: 97.5
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 5..72 e-value: 1.9E-22 score: 79.2
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 5..75 score: 19.733528
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 5..64 e-value: 1.51549E-23 score: 86.0601
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 1..86 e-value: 2.2E-24 score: 87.4
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 2..73
None	No IPR available	PANTHER	PTHR24074:SF34	CHAPERONE PROTEIN DNAJ 72	coord: 5..175
None	No IPR available	PANTHER	PTHR24074	CO-CHAPERONE PROTEIN DJLA	coord: 5..175
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 52..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0280.1	MC01g0280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane