MC00g0059 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0059
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	ENDO3c domain-containing protein
Location	scaffold31: 1251984 .. 1252239 (-)
RNA-Seq Expression	MC00g0059
Synteny	MC00g0059

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGCTAAAGACATTGACGGCTGTGAAGGGAATCGATCTTTGGTTGGTGTATATATTCTTGATTTTTAACCTGCACCAGTCGAACGTGCTGCCAGTGGCCGATGGGAGATCTCGGCGTCGGAAAAGGCGTGCGACCGCAGTACGTGAAATGGGATGGAGAAACTGTGAAGGTGGAGATGGAGATGGAAAAACTATGCGAGAAATGGGAGCCGTACACGTCGATGGGAGCTTGGTACACGTGGAGGCTAATTCGTAAT

mRNA sequence

CTGCTAAAGACATTGACGGCTGTGAAGGGAATCGATCTTTGGTTGGTGTATATATTCTTGATTTTTAACCTGCACCAGTCGAACGTGCTGCCAGTGGGAGATCTCGGCGTCGGAAAAGGCGTGCGACCGCAGTACGTGAAATGGGATGGAGAAACTGTGAAGGTGGAGATGGAGATGGAAAAACTATGCGAGAAATGGGAGCCGTACACGTCGATGGGAGCTTGGTACACGTGGAGGCTAATTCGTAAT

Coding sequence (CDS)

CTGCTAAAGACATTGACGGCTGTGAAGGGAATCGATCTTTGGTTGGTGTATATATTCTTGATTTTTAACCTGCACCAGTCGAACGTGCTGCCAGTGGGAGATCTCGGCGTCGGAAAAGGCGTGCGACCGCAGTACGTGAAATGGGATGGAGAAACTGTGAAGGTGGAGATGGAGATGGAAAAACTATGCGAGAAATGGGAGCCGTACACGTCGATGGGAGCTTGGTACACGTGGAGGCTAATTCGTAAT

Protein sequence

LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEMEKLCEKWEPYTSMGAWYTWRLIRN

Homology

[Close]

BLAST of MC00g0059 vs. ExPASy Swiss-Prot
Match: Q92383 (DNA-3-methyladenine glycosylase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mag1 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.4e-05
Identity = 26/79 (32.91%), Postives = 43/79 (54.43%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           L++ LT +KGI  W V + LIF+L++ +V+P  DL +  G R  +       +  +M + 
Sbjct: 137 LIERLTQIKGIGRWTVEMLLIFSLNRDDVMPADDLSIRNGYRYLHRL---PKIPTKMYVL 196

Query: 61  KLCEKWEPYTSMGAWYTWR 80
           K  E   P+ +  AWY W+
Sbjct: 197 KHSEICAPFRTAAAWYLWK 212

[Close]

BLAST of MC00g0059 vs. NCBI nr
Match: XP_038881017.1 (DNA-3-methyladenine glycosylase 1-like [Benincasa hispida])

HSP 1 Score: 102 bits (253), Expect = 4.12e-24
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           LL+ LTAVKGI +W V++F+IF LH+ +VLPVGDLGV KGV+  Y     + +    EME
Sbjct: 196 LLEALTAVKGIGMWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPAEME 255

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLCEKW+PY SMGAWY WRL+
Sbjct: 256 KLCEKWKPYRSMGAWYMWRLM 273

[Close]

BLAST of MC00g0059 vs. NCBI nr
Match: XP_004143510.1 (DNA-3-methyladenine glycosylase 1 [Cucumis sativus] >KGN48831.1 hypothetical protein Csa_003752 [Cucumis sativus])

HSP 1 Score: 101 bits (252), Expect = 6.58e-24
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           LL+ LTAVKGI +W V++F+IF LH+ +VLPVGDLGV KGV+  Y     + +    EME
Sbjct: 196 LLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPAEME 255

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLCEKW+PY S+GAWY WRLI
Sbjct: 256 KLCEKWKPYRSIGAWYMWRLI 273

[Close]

BLAST of MC00g0059 vs. NCBI nr
Match: XP_022949777.1 (DNA-3-methyladenine glycosylase 1-like [Cucurbita moschata])

HSP 1 Score: 102 bits (253), Expect = 9.38e-24
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           LL  LTAVKGI +W V++F+IF LH+ +VLPVGDLGV KGV+  Y     + +   +EME
Sbjct: 225 LLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPVEME 284

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLCEKW+PY SMGAWY WRL+
Sbjct: 285 KLCEKWKPYRSMGAWYMWRLM 302

[Close]

BLAST of MC00g0059 vs. NCBI nr
Match: KAG7034142.1 (mag1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 102 bits (253), Expect = 9.38e-24
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           LL  LTAVKGI +W V++F+IF LH+ +VLPVGDLGV KGV+  Y     + +   +EME
Sbjct: 225 LLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPVEME 284

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLCEKW+PY SMGAWY WRL+
Sbjct: 285 KLCEKWKPYRSMGAWYMWRLM 302

[Close]

BLAST of MC00g0059 vs. NCBI nr
Match: XP_022132520.1 (DNA-3-methyladenine glycosylase 1-like [Momordica charantia])

HSP 1 Score: 101 bits (252), Expect = 1.01e-23
Identity = 48/81 (59.26%), Postives = 63/81 (77.78%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           L++TLTAVKGI +W V +F+IF+LH+ +VLPVGDLGV KGV+  Y     + +   +EME
Sbjct: 208 LVRTLTAVKGIGVWSVQMFMIFSLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPVEME 267

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLC+KW+PY SMGAWY WRL+
Sbjct: 268 KLCDKWKPYRSMGAWYMWRLM 285

[Close]

BLAST of MC00g0059 vs. ExPASy TrEMBL
Match: A0A0A0KM62 (ENDO3c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G502690 PE=4 SV=1)

HSP 1 Score: 101 bits (252), Expect = 3.18e-24
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           LL+ LTAVKGI +W V++F+IF LH+ +VLPVGDLGV KGV+  Y     + +    EME
Sbjct: 196 LLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPAEME 255

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLCEKW+PY S+GAWY WRLI
Sbjct: 256 KLCEKWKPYRSIGAWYMWRLI 273

[Close]

BLAST of MC00g0059 vs. ExPASy TrEMBL
Match: A0A6J1GD23 (DNA-3-methyladenine glycosylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453068 PE=4 SV=1)

HSP 1 Score: 102 bits (253), Expect = 4.54e-24
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           LL  LTAVKGI +W V++F+IF LH+ +VLPVGDLGV KGV+  Y     + +   +EME
Sbjct: 225 LLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPVEME 284

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLCEKW+PY SMGAWY WRL+
Sbjct: 285 KLCEKWKPYRSMGAWYMWRLM 302

[Close]

BLAST of MC00g0059 vs. ExPASy TrEMBL
Match: A0A6J1BSP4 (DNA-3-methyladenine glycosylase 1-like OS=Momordica charantia OX=3673 GN=LOC111005358 PE=4 SV=1)

HSP 1 Score: 101 bits (252), Expect = 4.88e-24
Identity = 48/81 (59.26%), Postives = 63/81 (77.78%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           L++TLTAVKGI +W V +F+IF+LH+ +VLPVGDLGV KGV+  Y     + +   +EME
Sbjct: 208 LVRTLTAVKGIGVWSVQMFMIFSLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPVEME 267

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLC+KW+PY SMGAWY WRL+
Sbjct: 268 KLCDKWKPYRSMGAWYMWRLM 285

[Close]

BLAST of MC00g0059 vs. ExPASy TrEMBL
Match: A0A6J1IQD1 (DNA-3-methyladenine glycosylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478481 PE=4 SV=1)

HSP 1 Score: 100 bits (249), Expect = 1.79e-23
Identity = 48/81 (59.26%), Postives = 60/81 (74.07%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           LL  LT VKGI +W V++F+IF LH+ +VLPVGDLGV KGV+  Y     + +   +EME
Sbjct: 225 LLSALTGVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPVEME 284

Query: 61  KLCEKWEPYTSMGAWYTWRLI 81
           KLCEKW+PY SMGAWY WRL+
Sbjct: 285 KLCEKWKPYRSMGAWYMWRLM 302

[Close]

BLAST of MC00g0059 vs. ExPASy TrEMBL
Match: A0A5A7T3R0 (Putative DNA-3-methyladenine glycosylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002080 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.00e-23
Identity = 48/83 (57.83%), Postives = 60/83 (72.29%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           LL  LTAVKGI +W V++F+IF LH+ +VLPVGDLGV KGV+  Y     + +    EME
Sbjct: 196 LLGELTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL---KELPKPAEME 255

Query: 61  KLCEKWEPYTSMGAWYTWRLIRN 83
           KLCEKW+PY S+GAWY WRL+  
Sbjct: 256 KLCEKWKPYRSIGAWYMWRLMEE 275

[Close]

BLAST of MC00g0059 vs. TAIR 10
Match: AT3G50880.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 1.2e-20
Identity = 44/83 (53.01%), Postives = 61/83 (73.49%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           L+  LT VKGI +W V++F+IF+LH+ +VLPVGDLGV KGV+  Y     + +   ++ME
Sbjct: 190 LIDRLTLVKGIGVWTVHMFMIFSLHRPDVLPVGDLGVRKGVKDLY---GLKNLPGPLQME 249

Query: 61  KLCEKWEPYTSMGAWYTWRLIRN 84
           +LCEKW PY S+G+WY WRLI +
Sbjct: 250 QLCEKWRPYRSVGSWYMWRLIES 269

[Close]

BLAST of MC00g0059 vs. TAIR 10
Match: AT1G75230.2 (DNA glycosylase superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-15
Identity = 40/83 (48.19%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           L   LT V GI  W V++F+I +LH+ +VLPV DLGV KGV+   +    E +    +ME
Sbjct: 253 LFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQ---MLNGMEDLPRPSKME 312

Query: 61  KLCEKWEPYTSMGAWYTWRLIRN 84
           +LCEKW PY S+ +WY WRLI +
Sbjct: 313 QLCEKWRPYRSVASWYLWRLIES 332

[Close]

BLAST of MC00g0059 vs. TAIR 10
Match: AT1G75230.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-15
Identity = 40/83 (48.19%), Postives = 54/83 (65.06%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           L   LT V GI  W V++F+I +LH+ +VLPV DLGV KGV+   +    E +    +ME
Sbjct: 253 LFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQ---MLNGMEDLPRPSKME 312

Query: 61  KLCEKWEPYTSMGAWYTWRLIRN 84
           +LCEKW PY S+ +WY WRLI +
Sbjct: 313 QLCEKWRPYRSVASWYLWRLIES 332

[Close]

BLAST of MC00g0059 vs. TAIR 10
Match: AT1G19480.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 2.7e-15
Identity = 40/81 (49.38%), Postives = 52/81 (64.20%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           L   LT V GI  W V++F+I +LH+ +VLPV DLGV KGV+  Y   D   +    +ME
Sbjct: 257 LFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGLDD---LPRPSQME 316

Query: 61  KLCEKWEPYTSMGAWYTWRLI 82
           + C KW PY S+G+WY WRLI
Sbjct: 317 QHCAKWRPYRSVGSWYMWRLI 334

[Close]

BLAST of MC00g0059 vs. TAIR 10
Match: AT1G19480.2 (DNA glycosylase superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 2.7e-15
Identity = 40/81 (49.38%), Postives = 52/81 (64.20%), Query Frame = 0


Query: 1   LLKTLTAVKGIDLWLVYIFLIFNLHQSNVLPVGDLGVGKGVRPQYVKWDGETVKVEMEME 60
           L   LT V GI  W V++F+I +LH+ +VLPV DLGV KGV+  Y   D   +    +ME
Sbjct: 257 LFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGLDD---LPRPSQME 316

Query: 61  KLCEKWEPYTSMGAWYTWRLI 82
           + C KW PY S+G+WY WRLI
Sbjct: 317 QHCAKWRPYRSVGSWYMWRLI 334

The following BLAST results are available for this feature:

BLAST of MC00g0059 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q92383	1.4e-05	32.91	DNA-3-methyladenine glycosylase 1 OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]

BLAST of MC00g0059 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881017.1	4.12e-24	60.49	DNA-3-methyladenine glycosylase 1-like [Benincasa hispida]	[more]
XP_004143510.1	6.58e-24	60.49	DNA-3-methyladenine glycosylase 1 [Cucumis sativus] >KGN48831.1 hypothetical pro...	[more]
XP_022949777.1	9.38e-24	60.49	DNA-3-methyladenine glycosylase 1-like [Cucurbita moschata]	[more]
KAG7034142.1	9.38e-24	60.49	mag1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022132520.1	1.01e-23	59.26	DNA-3-methyladenine glycosylase 1-like [Momordica charantia]	[more]

BLAST of MC00g0059 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KM62	3.18e-24	60.49	ENDO3c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G502690 PE=4...	[more]
A0A6J1GD23	4.54e-24	60.49	DNA-3-methyladenine glycosylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1BSP4	4.88e-24	59.26	DNA-3-methyladenine glycosylase 1-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IQD1	1.79e-23	59.26	DNA-3-methyladenine glycosylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114784...	[more]
A0A5A7T3R0	4.00e-23	57.83	Putative DNA-3-methyladenine glycosylase 2 OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of MC00g0059 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50880.1	1.2e-20	53.01	DNA glycosylase superfamily protein	[more]
AT1G75230.2	1.2e-15	48.19	DNA glycosylase superfamily protein	[more]
AT1G75230.1	1.2e-15	48.19	DNA glycosylase superfamily protein	[more]
AT1G19480.1	2.7e-15	49.38	DNA glycosylase superfamily protein	[more]
AT1G19480.2	2.7e-15	49.38	DNA glycosylase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.1670.40		coord: 27..79 e-value: 3.1E-21 score: 77.7
None	No IPR available	GENE3D	1.10.340.30	Hypothetical protein; domain 2	coord: 2..26 e-value: 3.1E-21 score: 77.7
None	No IPR available	PANTHER	PTHR43003:SF5	DNA-3-METHYLADENINE GLYCOSYLASE	coord: 1..82
None	No IPR available	PANTHER	PTHR43003	DNA-3-METHYLADENINE GLYCOSYLASE	coord: 1..82
IPR011257	DNA glycosylase	SUPERFAMILY	48150	DNA-glycosylase	coord: 2..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g0059.1	MC00g0059.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006285	base-excision repair, AP site formation
biological_process	GO:0006307	DNA dealkylation involved in DNA repair
biological_process	GO:0006281	DNA repair
cellular_component	GO:0005634	nucleus
cellular_component	GO:0032993	protein-DNA complex
molecular_function	GO:0032131	alkylated DNA binding
molecular_function	GO:0008725	DNA-3-methyladenine glycosylase activity
molecular_function	GO:0043916	DNA-7-methylguanine glycosylase activity
molecular_function	GO:0003824	catalytic activity