Lsi11G005220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G005220
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	kunitz trypsin inhibitor 1-like
Location	chr11: 5497447 .. 5498034 (-)
RNA-Seq Expression	Lsi11G005220
Synteny	Lsi11G005220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGATGTTGCATAAGTCACTTGCTATCTTTAGCTACATATGCCTCTTCATGGCCAGAACTTCCACAGCTCAACTTCCTCCGGTGCTTGACACCGACGGTCAACCTCTACGACGCGGTGTCGAGTACTACATCAAGCCTGCAATTACCGACGTTGCAGGTAACCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTACGTCGGCCAAGAATCAGTTACTTCTACAAATATCGGGCTTCCGGTCACCTTCACTCCCATCGTAGCTGGGGAAGATATAATTGAAGAAAGTAGGGGTTTGAACGTTGTGTTTCAAGCGTCGTCGACGTGCATAATGTCGACCCAATGGAGAGTAGATGAGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGACCGGGAATGATGATGGTCCTACCGGGGTCTTTAGGATCGATAGGAACAATGGAGTTTACAGTATAGTATGGTGTCCTGAGATGATGGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTTGAGAATGGAGTAAGGCTGTTGGCTTTGGATGGTGATGCATTTCCTTTTGAGTTTGTGAAAGCTTGA

mRNA sequence

ATGATGATGTTGCATAAGTCACTTGCTATCTTTAGCTACATATGCCTCTTCATGGCCAGAACTTCCACAGCTCAACTTCCTCCGGTGCTTGACACCGACGGTCAACCTCTACGACGCGGTGTCGAGTACTACATCAAGCCTGCAATTACCGACGTTGCAGGTAACCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTACGTCGGCCAAGAATCAGTTACTTCTACAAATATCGGGCTTCCGGTCACCTTCACTCCCATCGTAGCTGGGGAAGATATAATTGAAGAAAGTAGGGGTTTGAACGTTGTGTTTCAAGCGTCGTCGACGTGCATAATGTCGACCCAATGGAGAGTAGATGAGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGACCGGGAATGATGATGGTCCTACCGGGGTCTTTAGGATCGATAGGAACAATGGAGTTTACAGTATAGTATGGTGTCCTGAGATGATGGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTTGAGAATGGAGTAAGGCTGTTGGCTTTGGATGGTGATGCATTTCCTTTTGAGTTTGTGAAAGCTTGA

Coding sequence (CDS)

ATGATGATGTTGCATAAGTCACTTGCTATCTTTAGCTACATATGCCTCTTCATGGCCAGAACTTCCACAGCTCAACTTCCTCCGGTGCTTGACACCGACGGTCAACCTCTACGACGCGGTGTCGAGTACTACATCAAGCCTGCAATTACCGACGTTGCAGGTAACCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTACGTCGGCCAAGAATCAGTTACTTCTACAAATATCGGGCTTCCGGTCACCTTCACTCCCATCGTAGCTGGGGAAGATATAATTGAAGAAAGTAGGGGTTTGAACGTTGTGTTTCAAGCGTCGTCGACGTGCATAATGTCGACCCAATGGAGAGTAGATGAGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGACCGGGAATGATGATGGTCCTACCGGGGTCTTTAGGATCGATAGGAACAATGGAGTTTACAGTATAGTATGGTGTCCTGAGATGATGGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTTGAGAATGGAGTAAGGCTGTTGGCTTTGGATGGTGATGCATTTCCTTTTGAGTTTGTGAAAGCTTGA

Protein sequence

MMMLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDEAESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLALDGDAFPFEFVKA

Homology

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BLAST of Lsi11G005220 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.5e-32
Identity = 85/204 (41.67%), Postives = 118/204 (57.84%), Query Frame = 0


Query: 3   MLHKSLAIF--SYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKS 62
           M  +SL IF  +++ L MA TS AQ   V+DT G+P+    EY+I+PAIT   G  TL +
Sbjct: 1   MSTRSLTIFILAHVWLLMATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 63  RSNAPCPLYVGQESVTSTNIGLPVTFTPIVA--GEDIIEESRGLNVVFQASSTCIMSTQW 122
             N PCPL+VG ++   T +G+ V FTP      +D +  +R L V F  S++C  ST W
Sbjct: 61  -GNGPCPLHVGLDNTEGT-LGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 123 RVDEAESDTGRRFVGTGNDDG---PTGVFRI--DRNNGVYSIVWCPE---MMGRPRCGSA 182
           R+ E ++ +GRR + TG D+G       FRI   +  G Y+I WCP       + +CG+ 
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 183 GILVENGVRLLALDGDAFPFEFVK 195
           G++ ENG  LLALDGDA P  F K
Sbjct: 181 GVIRENGKNLLALDGDALPVVFQK 200

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BLAST of Lsi11G005220 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.5e-32
Identity = 84/205 (40.98%), Postives = 117/205 (57.07%), Query Frame = 0


Query: 1   MMMLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKS 60
           M +  ++L I +++CLF+  T+ AQ   VLDT G+P+    EY+I+P IT+  G  T+ S
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVS 62

Query: 61  RSNAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDI--IEESRGLNVVFQASSTCIMSTQW 120
           R N  CPL+VG E +T    GL V FTP     D   +  +R L + FQASS+C+ ST+W
Sbjct: 63  R-NESCPLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEW 122

Query: 121 RVDEAESDTGRRFVGTGNDDGPTG----VFRIDRN--NGVYSIVWCPEMM---GRPRCGS 180
           R+ E ++ +GRR + TG D    G     FRI      G+Y+I WCP  +    +  CG+
Sbjct: 123 RLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGT 182

Query: 181 AGILVENGVRLLALDGDAFPFEFVK 195
             +L ENG  LLALDG   P  F K
Sbjct: 183 VDMLNENGKILLALDGGPLPLVFQK 203

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BLAST of Lsi11G005220 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.5e-29
Identity = 80/205 (39.02%), Postives = 117/205 (57.07%), Query Frame = 0


Query: 1   MMMLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKS 60
           M  +  ++ I + + L +  TS AQ   V+DT G+P+    +Y+I+PAIT   G+LTL +
Sbjct: 1   MSTISFTIFILANVWLLVVTTSIAQF--VIDTSGEPVENDEDYFIRPAITGNGGSLTLVT 60

Query: 61  RSNAPCPLYVGQESVTSTNIGLPVTFTPIVAG--EDIIEESRGLNVVFQASSTCIMSTQW 120
           R++  CP  VG +       G  V  +P V+   ED +   R L V+FQA ++C  ST+W
Sbjct: 61  RNS--CPFNVGLD--PDAPQGFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEW 120

Query: 121 RVDEAESDTGRRFVGTGNDDGPTG----VFRIDR--NNGVYSIVWCPEMM---GRPRCGS 180
           R+ E ++ TGRRF+ TG DD   G     FRI +  + G+++I WCP  +    +  CG+
Sbjct: 121 RLGERDATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGT 180

Query: 181 AGILVENGVRLLALDGDAFPFEFVK 195
            GI+ ENG  LLALDG A P  F K
Sbjct: 181 VGIVRENGKILLALDGSALPVAFQK 199

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BLAST of Lsi11G005220 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-19
Identity = 66/183 (36.07%), Postives = 92/183 (50.27%), Query Frame = 0


Query: 28  PVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKS---RSNAPCPLYVGQESVTSTNIGLPV 87
           PVLD DG+ LR G  YYI P + D  G LT+ +        CP  V Q +    +   P+
Sbjct: 35  PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQ-TRKEVDHDRPL 94

Query: 88  TFTPIVAGEDIIEESRGLNVVFQASSTC--IMSTQWRVDEAESDTGRRFV---GTGNDDG 147
            F P    ED++  S  LN+ F A   C    ST WR+D+ +  TG+ FV   G   + G
Sbjct: 95  AFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPG 154

Query: 148 PTGV---FRIDR--NNGVYSIVWCPEMMG--RPRCGSAGILV-ENGVRLLALDGDAFPFE 195
           P  +   F+I+    +G Y +V+CP + G  + +CG  GI + + G R LAL    F FE
Sbjct: 155 PETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFE 214

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BLAST of Lsi11G005220 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 2.1e-15
Identity = 54/153 (35.29%), Postives = 77/153 (50.33%), Query Frame = 0


Query: 24  AQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQESVTSTNIGLP 83
           A  PPV DTDG  LR    YY+  A     G LT+       CPL+V Q+     + G P
Sbjct: 23  ADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHDGFP 82

Query: 84  VTFTP--IVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDEAESDTGRRFVGTG--NDDG 143
           V  TP  +   + II  S  + + F+A +TC+ ST+W +D +E   GRR V TG   D  
Sbjct: 83  VRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHID-SELAAGRRHVITGPVKDPS 142

Query: 144 PTG---VFRIDRNNGV----YSIV----WCPEM 162
           P+G    FRI++ +G     Y ++    WC ++
Sbjct: 143 PSGRENAFRIEKYSGAEVHEYKLMSCGDWCQDL 173

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BLAST of Lsi11G005220 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 6.2e-95
Identity = 172/193 (89.12%), Postives = 182/193 (94.30%), Query Frame = 0


Query: 3   MLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAIFS++ +FMA TSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE + STNIGLPVTFTPIVAGEDIIEES G NVVFQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLA 182
            ES TGRRFVG GND+GPTG+FRIDRNNGVY+IVWCPEM+GRPRCGSAGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKA 196
           LDG+AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Lsi11G005220 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 9.0e-94
Identity = 170/193 (88.08%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 3   MLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAIFSY+ +F+A TSTAQLPPVLDTDGQPLRRGVEYYIKPAIT+VAGNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 63  NAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE V STNIGLPVTFTPIVAGEDIIEES G NV FQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLA 182
            ES TGRRFVG GND+GPTG+FRIDRNNGVY+IVWCPEM+GRPRCGSAGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKA 196
           LDG+AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Lsi11G005220 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 8.2e-87
Identity = 157/195 (80.51%), Postives = 170/195 (87.18%), Query Frame = 0


Query: 1   MMMLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKS 60
           MMMLHKSLAIF Y CLFMA TSTAQ PPVLDT+GQPLRRGVEYYI PAITDV GNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRV 120
           RSNAPCPL+VGQE VTSTNIGLPVTF P  AG+DII+E   LN+VF+A STC  STQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 120

Query: 121 DEAESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRL 180
           D  ESDTGRRFVG G++DGP G+F I R+NG Y+IVWCP MMGRPRCG AGILVENGVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGDAFPFEFVKA 196
           +ALDGDAFPFEF+KA
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of Lsi11G005220 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 6.9e-86
Identity = 155/193 (80.31%), Postives = 168/193 (87.05%), Query Frame = 0


Query: 3   MLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLHKSLAIF Y CLFMA TSTAQ PPVLDT+GQPLRRGVEYYI PAITDV GNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 63  NAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDE 122
           NAPCPL+VGQE VTSTNIGLPVTF P  AG+DII+E   LN+VF+A STC  STQWRVD 
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLA 182
            ESDTGRRFVG G++DGP G+F I R+NG Y+IVWCP MMGRPRCG AGILVENGVRL+A
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 183 LDGDAFPFEFVKA 196
           LDGDAFPFEF+KA
Sbjct: 181 LDGDAFPFEFIKA 193

[Close]

BLAST of Lsi11G005220 vs. ExPASy TrEMBL
Match: A0A0A0LIK2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.8e-78
Identity = 142/178 (79.78%), Postives = 155/178 (87.08%), Query Frame = 0


Query: 18  MARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQESVTS 77
           MA TSTAQLPPVLDT+GQPL+RGVEYYI PAITDV GNLTLKSRSNAPCPL+VGQE VTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 78  TNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDEAESDTGRRFVGTGND 137
           TNIGLPVTF P   G DII+E   LN+VFQA STC+ STQWRVD  ESDTGRRFVG G++
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 138 DGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLALDGDAFPFEFVKA 196
           DGP G+F I R+NG Y+IVWCP MMGRPRCG AGILVENGVRL+ALDGDAFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

[Close]

BLAST of Lsi11G005220 vs. NCBI nr
Match: XP_038888526.1 (kunitz type trypsin inhibitor 106-like [Benincasa hispida])

HSP 1 Score: 367.9 bits (943), Expect = 5.6e-98
Identity = 177/195 (90.77%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 1   MMMLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKS 60
           MMMLHKSLAIF Y CLFMA TSTAQLPPVLDTD QPLRRGVEYYIKPAITDV GNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTDSQPLRRGVEYYIKPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRV 120
           RSNAPCPL+VGQE VTSTNIGLPVTFTPIVAGEDIIEESR LN+VF+A STC+ STQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIVAGEDIIEESRSLNIVFEALSTCVTSTQWRV 120

Query: 121 DEAESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRL 180
           DEAESDTGRRFVG GNDDGP+G+FRIDRNNGVY+IVWCP MMGRPRCGSAGILVENGVRL
Sbjct: 121 DEAESDTGRRFVGIGNDDGPSGIFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVENGVRL 180

Query: 181 LALDGDAFPFEFVKA 196
           LALDGDAFPFEFVKA
Sbjct: 181 LALDGDAFPFEFVKA 195

[Close]

BLAST of Lsi11G005220 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 356.7 bits (914), Expect = 1.3e-94
Identity = 172/193 (89.12%), Postives = 182/193 (94.30%), Query Frame = 0


Query: 3   MLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAIFS++ +FMA TSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE + STNIGLPVTFTPIVAGEDIIEES G NVVFQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLA 182
            ES TGRRFVG GND+GPTG+FRIDRNNGVY+IVWCPEM+GRPRCGSAGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKA 196
           LDG+AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Lsi11G005220 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 353.2 bits (905), Expect = 1.4e-93
Identity = 171/193 (88.60%), Postives = 180/193 (93.26%), Query Frame = 0


Query: 3   MLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAIFSY+ +FMA TSTAQLPPVLDTDGQPL+RGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE V STNIGLPVTFTPIVAGEDIIEES G NV FQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLA 182
            ES TGRRFVG GND+ PTG+FRIDRNNGVY+IVWCPEM+GRPRCGSAGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKA 196
           LDG+AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of Lsi11G005220 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 352.8 bits (904), Expect = 1.9e-93
Identity = 170/193 (88.08%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 3   MLHKSLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAIFSY+ +F+A TSTAQLPPVLDTDGQPLRRGVEYYIKPAIT+VAGNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 63  NAPCPLYVGQESVTSTNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE V STNIGLPVTFTPIVAGEDIIEES G NV FQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLA 182
            ES TGRRFVG GND+GPTG+FRIDRNNGVY+IVWCPEM+GRPRCGSAGILVE+GVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKA 196
           LDG+AFPFEFVKA
Sbjct: 181 LDGNAFPFEFVKA 193

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BLAST of Lsi11G005220 vs. NCBI nr
Match: KAG7030262.1 (hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.8 bits (865), Expect = 6.2e-89
Identity = 162/178 (91.01%), Postives = 169/178 (94.94%), Query Frame = 0


Query: 18  MARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQESVTS 77
           MA TSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQE + S
Sbjct: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60

Query: 78  TNIGLPVTFTPIVAGEDIIEESRGLNVVFQASSTCIMSTQWRVDEAESDTGRRFVGTGND 137
           TNIGLPVTFTPIVAGEDIIEES G NVVFQASSTCI STQWRVDE ES TGRRFVG GND
Sbjct: 61  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 120

Query: 138 DGPTGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILVENGVRLLALDGDAFPFEFVKA 196
           +GPTG+FRIDRNNGVY+IVWCPEM+GRPRCGSAGILVE+GVRLLALDG+AFPFEFVKA
Sbjct: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 178

[Close]

BLAST of Lsi11G005220 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 68.9 bits (167), Expect = 5.0e-12
Identity = 56/196 (28.57%), Postives = 92/196 (46.94%), Query Frame = 0


Query: 7   SLAIFSYICLFMARTSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPC 66
           +L + S I    +         VLD  G P++  V+YYI PA     G L   +R+ +  
Sbjct: 10  TLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQ 69

Query: 67  PLYVGQESVTSTN---IGLPVTFTPIVAGEDIIEESRGLNVVFQASS-TCIMSTQWRVDE 126
            L +  + V S++    GLPVTF+P+      ++ S  LN+ F ++   C  S  WR+D 
Sbjct: 70  DLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDH 129

Query: 127 AESDTGRRFVGTGNDDGP-TGVFRIDRNNGVYSIVWCPEMMGRPRCGSAGILV-ENGVRL 186
           +     + FV  G   G     F+I  +   Y +++CP +     C +  + + ++GVR 
Sbjct: 130 S-VQLRKSFVSIGGQKGKGNSWFQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRR 189

Query: 187 LALDGD-AFPFEFVKA 196
           L L  D +F  +F KA
Sbjct: 190 LVLSTDQSFVVKFQKA 203

The following BLAST results are available for this feature:

BLAST of Lsi11G005220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A072TH68	2.5e-32	41.67	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV1	2.5e-32	40.98	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
G7LCV7	1.5e-29	39.02	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
P13087	1.1e-19	36.07	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P07596	2.1e-15	35.29	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of Lsi11G005220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G3G3	6.2e-95	89.12	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	9.0e-94	88.08	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]
A0A5D3D468	8.2e-87	80.51	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A5A7VDV8	6.9e-86	80.31	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A0A0LIK2	1.8e-78	79.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=3 SV=1	[more]

BLAST of Lsi11G005220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888526.1	5.6e-98	90.77	kunitz type trypsin inhibitor 106-like [Benincasa hispida]	[more]
XP_022946294.1	1.3e-94	89.12	miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo...	[more]
XP_023545477.1	1.4e-93	88.60	miraculin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022999136.1	1.9e-93	88.08	miraculin-like [Cucurbita maxima]	[more]
KAG7030262.1	6.2e-89	91.01	hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lsi11G005220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73325.1	5.0e-12	28.57	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 28..195 e-value: 6.6E-31 score: 118.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 29..194 e-value: 1.3E-42 score: 145.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..194
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 29..45
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 28..194 e-value: 3.94358E-40 score: 132.851
None	No IPR available	GENE3D	2.80.10.50		coord: 26..195 e-value: 1.8E-47 score: 163.1
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 4..194
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G005220.1	Lsi11G005220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0003953	NAD+ nucleosidase activity