Lsi09G018730 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G018730
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr09: 27536826 .. 27538698 (+)
RNA-Seq Expression	Lsi09G018730
Synteny	Lsi09G018730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGTTTCCTTTAATCTACCCAACTACCTCTCTTTCCAATTCCACCGTCTTCAATGGAAGATATCCGCCAAAACCCTCCTCCTCTGCCGCAGCAACCGCCGCCACCGGCTGCCATCGTGGACTTTCCGCCATACTTCCGATGCCCAATTTCCATGGAGCTCATGCAAGATCCCGTCACGATTTCCACCGGGGTTTCGTTCGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCAGCAACCATGCAAACCATCGTCAACTTTGATATCACTCCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAGGAGTCTTGCTCCTCGTCCTCGTTGTTGCCGTCGTCGTCAATGGAGTATGATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCGCCGTTCAAGGCTAGTTCTTTGAAGAAACTACGGTTGCTCGTGGCAATGGATGATGTAGCTGCGGTCGGAGCGAAACCCGTCTTTGTTCGAGCAAATGGATTTGAAGTTCTTGTTGGAATAGTCACGCAAGTCGTCATTTGTGAGAGCTCCGATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTTTTGCAACACAAAACTCAAAAGCTTAATCTTTTTTTCTTTCTTAATGTTTAGCATGTAAGGGTGCACATTCAAACGTCGTCTATTTGGTCGAAGATATATATTATAACTGGTTGACTTATAACCTCATTTGGACAAAGATTTCAATTTTATTTATATTCTTAATAAAAAGATTATGTTTGAGAGTTTGAACGAGATGTAATAAATAACTATTGGTATTAGTTATAGATCGGGAAGTGACACATGGGAGTGCAACTCAAAATATCATTGAAGATTGCTATTAAATTCAAAAGATTAAGTTGATAGAATAGATTACGATAAATTCAATCATTTATTCATATTCTTAACAGGTATTATCTCAATTCCCATTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCGAAACCAGAAGCCATCAAATCAATGGCAATTGTATTACAAAGAGGAAGTATAGAAGGAAGGTTTTATGCCATGGAAATGCTTCAACAAATATCCAAAAAGGGATACGATTGGAACTCTTTAACAAAACATCATGCCATCGACTTGTTCAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCAAGTACAATTAAGACTACGGATTCCTTGATAATCATACAATGGTACGATATTGTCCACACATATTCATTTTATATAGCTAAGTTGACCATTTTTTCCTTTTTACTAAATCTTTCATTTTTTTCAGTCGATACGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCATCGAAAAAAAGTCGTCTTCTATCAATCGAGGCCGGTGCTGTTTGCGTCATGGTTGACCTTCTTCCCGACTCGAACCGATCAAAGTGCGAGAAAATATTACACATACTCAGACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGAGAGCATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGAGTGTGGGACAGTGAAGAAGCTTCTAGGGATCTTGAATGGTGATGGAAGACCTTCAACCAAGGAGAAGGCCATGAAAATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGGGATTATTTGAAGTTGATAAATCAATAGAATATATGTTGAAGTGAATGAGAATTGGGTT

mRNA sequence

AATGTTTCCTTTAATCTACCCAACTACCTCTCTTTCCAATTCCACCGTCTTCAATGGAAGATATCCGCCAAAACCCTCCTCCTCTGCCGCAGCAACCGCCGCCACCGGCTGCCATCGTGGACTTTCCGCCATACTTCCGATGCCCAATTTCCATGGAGCTCATGCAAGATCCCGTCACGATTTCCACCGGGGTTTCGTTCGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCAGCAACCATGCAAACCATCGTCAACTTTGATATCACTCCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAGGAGTCTTGCTCCTCGTCCTCGTTGTTGCCGTCGTCGTCAATGGAGTATGATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCGCCGTTCAAGGCTAGTTCTTTGAAGAAACTACGGTTGCTCGTGGCAATGGATGATGTAGCTGCGGTCGGAGCGAAACCCGTCTTTGTTCGAGCAAATGGATTTGAAGTTCTTGTTGGAATAGTCACGCAAGTCGTCATTTGTGAGAGCTCCGATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTCCCATTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCGAAACCAGAAGCCATCAAATCAATGGCAATTGTATTACAAAGAGGAAGTATAGAAGGAAGGTTTTATGCCATGGAAATGCTTCAACAAATATCCAAAAAGGGATACGATTGGAACTCTTTAACAAAACATCATGCCATCGACTTGTTCAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCAAGTACAATTAAGACTACGGATTCCTTGATAATCATACAATGTCGATACGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCATCGAAAAAAAGTCGTCTTCTATCAATCGAGGCCGGTGCTGTTTGCGTCATGGTTGACCTTCTTCCCGACTCGAACCGATCAAAGTGCGAGAAAATATTACACATACTCAGACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGAGAGCATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGAGTGTGGGACAGTGAAGAAGCTTCTAGGGATCTTGAATGGTGATGGAAGACCTTCAACCAAGGAGAAGGCCATGAAAATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGGGATTATTTGAAGTTGATAAATCAATAGAATATATGTTGAAGTGAATGAGAATTGGGTT

Coding sequence (CDS)

ATGGAAGATATCCGCCAAAACCCTCCTCCTCTGCCGCAGCAACCGCCGCCACCGGCTGCCATCGTGGACTTTCCGCCATACTTCCGATGCCCAATTTCCATGGAGCTCATGCAAGATCCCGTCACGATTTCCACCGGGGTTTCGTTCGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAATAAGAAAACTTGCCCAGCAACCATGCAAACCATCGTCAACTTTGATATCACTCCAAATTATACGCTCAAAAGGCTCATACTTTCTTGGAAAATGAAGGAGTCTTGCTCCTCGTCCTCGTTGTTGCCGTCGTCGTCAATGGAGTATGATGATGTTGTGGCTGATCTCTTGAAGAACATCGAGTCGTCGCCGTTCAAGGCTAGTTCTTTGAAGAAACTACGGTTGCTCGTGGCAATGGATGATGTAGCTGCGGTCGGAGCGAAACCCGTCTTTGTTCGAGCAAATGGATTTGAAGTTCTTGTTGGAATAGTCACGCAAGTCGTCATTTGTGAGAGCTCCGATTTCGCTAATTTTGAAGCTTGCGAGGAGGCTTTAGGGGTATTATCTCAATTCCCATTTTCCAAAACCGAGAAGCCATTCGAGTTGCTATCGAAACCAGAAGCCATCAAATCAATGGCAATTGTATTACAAAGAGGAAGTATAGAAGGAAGGTTTTATGCCATGGAAATGCTTCAACAAATATCCAAAAAGGGATACGATTGGAACTCTTTAACAAAACATCATGCCATCGACTTGTTCAAATCAATATTGGAGCTTGCATCGGATGAGATAATCAAGAAAGCAAGTACAATTAAGACTACGGATTCCTTGATAATCATACAATGTCGATACGGGACATTGTTCTCGTCCGCCCTAGAGCTTTTGATAGAAATCATGGAATCATCGAAAAAAAGTCGTCTTCTATCAATCGAGGCCGGTGCTGTTTGCGTCATGGTTGACCTTCTTCCCGACTCGAACCGATCAAAGTGCGAGAAAATATTACACATACTCAGACTTCTAAGTGAGTGTGCAGAGGGAAGGTCAGCCATGGGAGAGCATATTATGGGAATAGCAACAGTGACAAAGAAAATGAGTATATCAAACACAGCTACAAAAATTGGGATGAAAATCTTGTGGTTGATTTGCAATTATCATCCTAGTGAGAGGGTTTTAGAAGAAATGATGGAGTGTGGGACAGTGAAGAAGCTTCTAGGGATCTTGAATGGTGATGGAAGACCTTCAACCAAGGAGAAGGCCATGAAAATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAGTTTAGGGATTATTTGAAGTTGATAAATCAATAG

Protein sequence

MEDIRQNPPPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLKNIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEIMESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ

Homology

[Close]

BLAST of Lsi09G018730 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 5.7e-48
Identity = 134/429 (31.24%), Postives = 219/429 (51.05%), Query Frame = 0


Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ PP+F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 82  YTLKRLILSWKMKESCSSSSLLPS-----SSMEYDDVVADLLKNIESSPFKASSLKKLRL 141
           +TL+RLI SW    +      +P+        E + ++ D   + E+   +   LK+LR 
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHEN---QVKCLKRLRQ 129

Query: 142 LVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEACEEALGVLSQFPFSK 201
           +V+ +       K     A   E L  IV       S+D  N    +EAL +L     S+
Sbjct: 130 IVSEN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSE 189

Query: 202 TEKPFELLSKPE--AIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAIDLF 261
           T     L +K +   +KS+  ++QRG  E R YA  +L+ I +      S+T     ++F
Sbjct: 190 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVF 249

Query: 262 KSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEIMESSKKSRLLSIEAG 321
             ++++  D I +KA+  K    +++  C +G                  ++R  ++EAG
Sbjct: 250 TEVVQILDDRISQKAT--KAAMHILVNICPWG------------------RNRHKAVEAG 309

Query: 322 AVCVMVDLLPD----SNRSKCEKILHILRLLSECAEGRSAMGEHIMGIATVTKK-MSISN 381
            + V+++LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S 
Sbjct: 310 VISVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQ 369

Query: 382 TATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEKAMKIMKLHGS 439
           TA+   +++L  +  +  +  +L EM++ G V KL  +L       TKEKA +++KLH  
Sbjct: 370 TASDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHAR 402

[Close]

BLAST of Lsi09G018730 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.0e-44
Identity = 130/453 (28.70%), Postives = 224/453 (49.45%), Query Frame = 0


Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ P +F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEY-DDVVADLLKNIESSPF-KASSLKKLRLLVA 141
           +TL+RLI SW    +      +P+         +  L+K   SS   +   LK+LR +V+
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 142 MDDVAAVGAKPVFVRANGFEVLVGIVTQVV-----------------ICESSDFAN-FEA 201
            +       K     A   E L  IV+  V                 +C+S+   N F++
Sbjct: 125 ENTT----NKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 184

Query: 202 ----CEEALGVLSQFPFSKTEKPFELLSK--PEAIKSMAIVLQRGSIEGRFYAMEMLQQI 261
                +EAL VL     S+T     L +K     +K++  ++QRG  E R YA  +L+++
Sbjct: 185 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 244

Query: 262 SKKGYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALEL 321
            +       +      +LF  ++++  D+I  KA+  ++   +++I C +G         
Sbjct: 245 LEVADPMQIILLER--ELFGEVIQILHDQISHKAT--RSAMQILVITCPWG--------- 304

Query: 322 LIEIMESSKKSRLLSIEAGAVCVMVDLLPD----SNRSKCEKILHILRLLSECAEGRSAM 381
                    ++R  ++E G + ++++LL D    S R   E  + +L +L +CAEGR+  
Sbjct: 305 ---------RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEF 364

Query: 382 GEHIMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNG 441
             H   IA V+KK + +S   ++  +++L  +  +  +  +L+EM++ G V KL  +L  
Sbjct: 365 LNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQV 424

Query: 442 DGRPSTKEKAMKIMKLHGSFWRRFPCFPYEFRD 444
                TKEKA +++KLH   WR  PC P    D
Sbjct: 425 SCGNKTKEKAKELLKLHARVWRESPCVPRNLYD 431

[Close]

BLAST of Lsi09G018730 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.2e-34
Identity = 119/445 (26.74%), Postives = 212/445 (47.64%), Query Frame = 0


Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 81
           ++ P YF CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 8   IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 67

Query: 82  NYTLKRLILSWKMKESCS-----SSSLLPSSSMEYDDVVADLLKNIESSPFKASSLKKLR 141
           N+ L+RLI  W ++         S+  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 68  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 127

Query: 142 LLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEACEEALGVLSQFPFS 201
           +L AMD       + +       + L+  V +    +       +  +E+L +L      
Sbjct: 128 VL-AMDG----NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 187

Query: 202 KTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAIDLFK 261
             +    L+     ++S+  VL +     + Y + +L+ +++  Y  + + +    ++FK
Sbjct: 188 SNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFK 247

Query: 262 SILELASDEI---------------------IKKASTIKTTDSLIIIQCRYGTLFSSALE 321
            I+    D +                     + K    K   SL+I Q       ++AL 
Sbjct: 248 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQA-----VTAALM 307

Query: 322 LLIEIMESSKKSRLLSIEAGAVCVMVDLLPDSNRSK--CEKILHILRLLSECAEGRSAMG 381
           +L+E   S  ++R L ++ GAV  +++L       K   E +L +L  L  CA GR+ + 
Sbjct: 308 ILLE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEIL 367

Query: 382 EHIMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGD 437
            H  GIA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D
Sbjct: 368 AHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD 427

[Close]

BLAST of Lsi09G018730 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.5e-32
Identity = 121/439 (27.56%), Postives = 205/439 (46.70%), Query Frame = 0


Query: 11  LPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATM 70
           +P+   P    +  P +FRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T 
Sbjct: 1   MPRNIEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTR 60

Query: 71  QTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDV-VADLLKNIE------- 130
             + +F + PN+TL+RLI  W +    +    +P+     D   V  LL           
Sbjct: 61  APLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHV 120

Query: 131 SSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEAC 190
           S   +A++L++LR      D   V    +    N  E+L+ I+        S+  + E  
Sbjct: 121 SVRSRAAALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELV 180

Query: 191 EEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQIS--KKGY 250
            E+L +L   P ++  +   + S P  ++ +  +L   SIE R  A  +++ +S   K  
Sbjct: 181 SESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSA 240

Query: 251 DWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEIM 310
           D    +  ++  +F+ +L+L  + I  + +          ++    TLF+         +
Sbjct: 241 DLKG-SISNSESVFEGVLDLLRNPISSRRA----------LKIGIKTLFA---------L 300

Query: 311 ESSKKSRLLSIEAGAVCVMVD-LLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIA 370
            S K +R ++I AGA  +++D L  D +R   E+ L  + LL    EG +A GEH + + 
Sbjct: 301 CSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVP 360

Query: 371 TVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKE 430
            + K  + +S+ AT+     L  +C     ER  EE    G V +LL ++  +     K+
Sbjct: 361 LLVKTILRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSECTERAKK 406

Query: 431 KAMKIMKLHGSFWRRFPCF 438
           KA K++KL    W  +  F
Sbjct: 421 KAQKLLKLLRDSWPDYNSF 406

[Close]

BLAST of Lsi09G018730 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.9e-28
Identity = 117/422 (27.73%), Postives = 195/422 (46.21%), Query Frame = 0


Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           +  P +FRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F + PN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDFTLIPN 71

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEYDDV-VADLLKNIE-------SSPFKASSLKK 141
           +TL+RLI  W +    +    +P+     D + V  LL           S   +A+++++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRR 131

Query: 142 LRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEACEEALGVLSQFP 201
           LR L    +   V    +    N  E+LV I+   +  E++  ++ E   E+L +L    
Sbjct: 132 LRGLARDSEKNRV----LIAGHNAREILVRILFADI--ETTSLSS-ELVSESLALLVLLH 191

Query: 202 FSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQI--SKKGYDWNSLTKHHAI 261
            ++TE    + S P  +  M  +L   SIE R  A  +++ +    K  D   L    + 
Sbjct: 192 MTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDL-KLIISGSD 251

Query: 262 DLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEIMESSKKSRLLSI 321
            +F+ +L+L  + I  + + +K     I   C                    K++R L+I
Sbjct: 252 SIFEGVLDLLKNPISSRRA-LKIGIKAIFALCLV------------------KQTRHLAI 311

Query: 322 EAGAVCVMVD-LLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIATVTKK-MSISN 381
            AGA  +++D L  D +R   E+ L  + LL    EG +A GEH + +  + K  + +S+
Sbjct: 312 SAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSD 371

Query: 382 TATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEKAMKIMKLHGS 432
            AT+     L  +C     ER  +E    G V +LL ++  D     K KA  ++KL   
Sbjct: 372 RATEYAAGALLALCT--AEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRD 402

[Close]

BLAST of Lsi09G018730 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.1e-230
Identity = 412/451 (91.35%), Postives = 426/451 (94.46%), Query Frame = 0


Query: 1   MEDIRQNP-PPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDI Q+P PPL    PPP A VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSS+SLLPSSSMEY DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANF 180
           NIESSPFKASSLKKLRLL+A+DDVAAVGAKPVFVR NGFE+LV IVTQV ICE SDFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAI+LQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEI 300
           YDWNSLTKH  IDLFKSILELASDEIIKKAS IKT DS  I QCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIA 360
           MESSKKSRLLSIEAGAVCVM+DLLPDSNRSKCEKIL+IL+LLSECAEGRSAMGEH MGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEK 420
           TVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMM CGTVKKLLGILNGDG  STKEK
Sbjct: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420

Query: 421 AMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           AMK+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]

BLAST of Lsi09G018730 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 9.0e-230
Identity = 417/453 (92.05%), Postives = 428/453 (94.48%), Query Frame = 0


Query: 1   MEDIRQNPPPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QNP P P  P P    VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNPFPPPHAPTP--TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSS-SSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSS SSLLPSSSMEY DVVADLL
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLL 120

Query: 121 KNIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFAN 180
           KNIESSPFKASSLKKLRLL+A+DDVAAVGAKPVFVR NGFEVL+GIVTQV ICESSDFAN
Sbjct: 121 KNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240

Query: 241 GYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIE 300
           GYDWNSLTKH  IDLFKSILELASDEIIKKAS IKTTDS I+  CRYGTLFSSALELLIE
Sbjct: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAVCVM+DLLPDSNRSKCEKILHIL+LLSECAEGRSAMGEHIMGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGIL-NGDGRPSTK 420
           ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMM CGTVKKLLGIL NGDGR STK
Sbjct: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           EKA K+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]

BLAST of Lsi09G018730 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 1.3e-215
Identity = 390/452 (86.28%), Postives = 413/452 (91.37%), Query Frame = 0


Query: 1   MEDIRQNPPPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIRQN  P PQ  PPP  + D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--SSSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  SSS     SMEYDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVA-LL 120

Query: 121 KNIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFAN 180
           KNIE SPFKASSLKKLRLL+A+DDV AVGAKP+FV+ANGFEVLVG+VTQ+ ICESS+FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSK EK FELL++PEAIKSMAIVLQRGSIEGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 GYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIE 300
           GYD+NSL+KHHAIDLFKSILELASDEIIKKAST+K TDS I   CRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAVC+M+DLLPDSNRSKCEKILH+L+LLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGR-PSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEM+ CGTVKKL+GILNGDGR  STK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 450
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Lsi09G018730 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.5e-213
Identity = 392/452 (86.73%), Postives = 411/452 (90.93%), Query Frame = 0


Query: 1   MEDIRQNPPPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIRQN  P    PP P A  D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLP----PPQPPAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--SSSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  SSS     S+EYDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVA-LL 120

Query: 121 KNIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFAN 180
           KNIE SPFKASSLKKLRLL+A+DDVAAV AK +FV+ANGFEVLVG+VTQV ICESS+FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEK FELLSK EAIKSM IVLQRGSIEGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKK 240

Query: 241 GYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIE 300
           GYD+NSL+KHHAIDLFKSILELASDEIIKKASTIKTTDS I  QCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGA+C+M+DLLPDSNRSKCEKILH+L+LLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGR-PSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMM CGTVKKL+GILNGDGR  STK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 450
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

[Close]

BLAST of Lsi09G018730 vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.0e-205
Identity = 377/451 (83.59%), Postives = 392/451 (86.92%), Query Frame = 0


Query: 1   MEDIRQNP-PPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDI Q+P PPL    PPP A VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSS+SLLPSSSMEY DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANF 180
           NIESSPFKASSLKKLRLL+A+DDVAAVGAKPVFVR NGFE+LV IVTQV ICE SDF NF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFTNF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAI+LQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEI 300
           YDWNSLTKH  IDLFKSILELASDEIIKKAS IKTTDS  I QCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIA 360
           MESSKKSRLLSIEAGAVCVM+DLLPDSNRSKCEKIL+IL+LLSECAEG            
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG------------ 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEK 420
                                   +YHPSERVLEEMM CGTVKKLLGILNGDG  STKEK
Sbjct: 361 ------------------------SYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 415

Query: 421 AMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           AMK+MKLHGSFWR+FPCFPYE+RDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRKFPCFPYEYRDYLKLINQ 415

[Close]

BLAST of Lsi09G018730 vs. NCBI nr
Match: XP_038875803.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 822.4 bits (2123), Expect = 1.9e-234
Identity = 418/450 (92.89%), Postives = 430/450 (95.56%), Query Frame = 0


Query: 1   MEDIRQNPPPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  P PQ PPP   +VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNLLPPPQPPPPSNTVVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLKN 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWK KES SSSS  PSSSM+YDDVVADLLKN
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKTKESSSSSSSSPSSSMKYDDVVADLLKN 120

Query: 121 IESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFE 180
           IES+PFKASSLKKLRLL+AMDDVAAVGAKPVFVRANGF+VLVGI+TQV ICES+DFANFE
Sbjct: 121 IESTPFKASSLKKLRLLMAMDDVAAVGAKPVFVRANGFDVLVGIITQVAICESADFANFE 180

Query: 181 ACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGY 240
           ACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAIVLQRGS+EGRFYAMEMLQQISKK Y
Sbjct: 181 ACEEALGVLSQFPFSKTEKPFELLSRPEAIKSMAIVLQRGSMEGRFYAMEMLQQISKKEY 240

Query: 241 DWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEIM 300
           DWNSLTKHH IDLFKSILELASDEIIKKASTIKTT+  II QCRYGTLFSSALELLIEIM
Sbjct: 241 DWNSLTKHHIIDLFKSILELASDEIIKKASTIKTTNFSIITQCRYGTLFSSALELLIEIM 300

Query: 301 ESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIAT 360
           ESSKKSRLLSIEAGAVCVM+DLLPDSNRSKCEKILHIL+LLSECAEGRSAMGEHIMGIAT
Sbjct: 301 ESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGIAT 360

Query: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEKA 420
           VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMM CGTVKKLLGILNGDGR STKEKA
Sbjct: 361 VTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNGDGRSSTKEKA 420

Query: 421 MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ
Sbjct: 421 MKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 450

[Close]

BLAST of Lsi09G018730 vs. NCBI nr
Match: XP_008460073.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 808.9 bits (2088), Expect = 2.2e-230
Identity = 412/451 (91.35%), Postives = 426/451 (94.46%), Query Frame = 0


Query: 1   MEDIRQNP-PPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFF 60
           MEDI Q+P PPL    PPP A VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDVVADLLK 120
           TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSS+SLLPSSSMEY DVVADLLK
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSSSMEYHDVVADLLK 120

Query: 121 NIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANF 180
           NIESSPFKASSLKKLRLL+A+DDVAAVGAKPVFVR NGFE+LV IVTQV ICE SDFANF
Sbjct: 121 NIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKG 240
           EACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAI+LQRGSIEGRFYAMEMLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISKKG 240

Query: 241 YDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEI 300
           YDWNSLTKH  IDLFKSILELASDEIIKKAS IKT DS  I QCRYGTLFSSALELLIEI
Sbjct: 241 YDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIA 360
           MESSKKSRLLSIEAGAVCVM+DLLPDSNRSKCEKIL+IL+LLSECAEGRSAMGEH MGIA
Sbjct: 301 MESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMGIA 360

Query: 361 TVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEK 420
           TVTKKMSISNTATKIGMKILW IC+YHPSERVLEEMM CGTVKKLLGILNGDG  STKEK
Sbjct: 361 TVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDGSSSTKEK 420

Query: 421 AMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           AMK+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 AMKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]

BLAST of Lsi09G018730 vs. NCBI nr
Match: XP_004145072.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical protein Csa_004999 [Cucumis sativus])

HSP 1 Score: 805.8 bits (2080), Expect = 1.9e-229
Identity = 417/453 (92.05%), Postives = 428/453 (94.48%), Query Frame = 0


Query: 1   MEDIRQNPPPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDI QNP P P  P P    VDFPP+FRCPISMELMQDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNPFPPPHAPTP--TTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK-ESCSS-SSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMK +SCSS SSLLPSSSMEY DVVADLL
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSSSMEYHDVVADLL 120

Query: 121 KNIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFAN 180
           KNIESSPFKASSLKKLRLL+A+DDVAAVGAKPVFVR NGFEVL+GIVTQV ICESSDFAN
Sbjct: 121 KNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEKPFELLS+PEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240

Query: 241 GYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIE 300
           GYDWNSLTKH  IDLFKSILELASDEIIKKAS IKTTDS I+  CRYGTLFSSALELLIE
Sbjct: 241 GYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGI 360
           IMESSKKSRLLSIEAGAVCVM+DLLPDSNRSKCEKILHIL+LLSECAEGRSAMGEHIMGI
Sbjct: 301 IMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGIL-NGDGRPSTK 420
           ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMM CGTVKKLLGIL NGDGR STK
Sbjct: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLINQ 451
           EKA K+MKLHGSFWRRFPCFPYE+RDYLKLINQ
Sbjct: 421 EKAKKVMKLHGSFWRRFPCFPYEYRDYLKLINQ 451

[Close]

BLAST of Lsi09G018730 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.7 bits (1981), Expect = 5.6e-218
Identity = 397/452 (87.83%), Postives = 416/452 (92.04%), Query Frame = 0


Query: 1   MEDIRQNPPPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIRQN  P  Q  PPP A+ D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--SSSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCS  SSS     SMEYDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVA-LL 120

Query: 121 KNIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFAN 180
           KNIE SPFKASSLKKLRLL+A+DDVAAVGAKP+FV+ANGFEVLVG+VTQV ICESS+FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSKTEK FELLS+PEAIKSMAIVLQRGSIEGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 GYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIE 300
           GYD+NSLTKHHAIDLFKSILELASDEIIKKAST+K TDS I  QCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAVC+M+DLLPDSNRSKCEKILH+L+LLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGR-PSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMM CGTVKKL+GILNGDGR  STK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 450
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Lsi09G018730 vs. NCBI nr
Match: KAG6574816.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 762.7 bits (1968), Expect = 1.8e-216
Identity = 392/452 (86.73%), Postives = 415/452 (91.81%), Query Frame = 0


Query: 1   MEDIRQNPPPLPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60
           MEDIRQN  P PQ  PPP  + D PPYFRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCS--SSSLLPSSSMEYDDVVADLL 120
           YNKKTCPATMQ IVNFDITPNYTLKRLI+SWKM ESCS  SSS     SMEYDDVVA LL
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVA-LL 120

Query: 121 KNIESSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFAN 180
           KNIE SPFKASSLKKLRLL+A+DDVAAVGAKP+FV+ANGFEVLVG+VTQ+ ICESS+FAN
Sbjct: 121 KNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFAN 180

Query: 181 FEACEEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKK 240
           FEACEEALGVLSQFPFSK EK FELL++PEAIKSMAIVLQRGSIEGRFYAME+LQQISKK
Sbjct: 181 FEACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKK 240

Query: 241 GYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIE 300
           GYD+NSL+KHHAIDLFKSILELASDEIIKKAST+K TDS I  QCRYGTLFSSALELLIE
Sbjct: 241 GYDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIE 300

Query: 301 IMESSKKSRLLSIEAGAVCVMVDLLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGI 360
           IMESSKK RLL IEAGAVC+M+DLLPDSNRSKCEKILH+L+LLS+CAEGR AM EHIMGI
Sbjct: 301 IMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGI 360

Query: 361 ATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGR-PSTK 420
           ATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMM CGTVKKL+GILNGDGR  STK
Sbjct: 361 ATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTK 420

Query: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYLKLIN 450
           EKAMKIMKLHGSFWRRFPCFPYEFRDY KLIN
Sbjct: 421 EKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Lsi09G018730 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 193.4 bits (490), Expect = 4.1e-49
Identity = 134/429 (31.24%), Postives = 219/429 (51.05%), Query Frame = 0


Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ PP+F CPIS+E+M+DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPN 69

Query: 82  YTLKRLILSWKMKESCSSSSLLPS-----SSMEYDDVVADLLKNIESSPFKASSLKKLRL 141
           +TL+RLI SW    +      +P+        E + ++ D   + E+   +   LK+LR 
Sbjct: 70  HTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHEN---QVKCLKRLRQ 129

Query: 142 LVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEACEEALGVLSQFPFSK 201
           +V+ +       K     A   E L  IV       S+D  N    +EAL +L     S+
Sbjct: 130 IVSEN----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSE 189

Query: 202 TEKPFELLSKPE--AIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAIDLF 261
           T     L +K +   +KS+  ++QRG  E R YA  +L+ I +      S+T     ++F
Sbjct: 190 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVF 249

Query: 262 KSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEIMESSKKSRLLSIEAG 321
             ++++  D I +KA+  K    +++  C +G                  ++R  ++EAG
Sbjct: 250 TEVVQILDDRISQKAT--KAAMHILVNICPWG------------------RNRHKAVEAG 309

Query: 322 AVCVMVDLLPD----SNRSKCEKILHILRLLSECAEGRSAMGEHIMGIATVTKK-MSISN 381
            + V+++LL D    S R   E  + +L LL +CAEGR+    H   IA V KK + +S 
Sbjct: 310 VISVIIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQ 369

Query: 382 TATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEKAMKIMKLHGS 439
           TA+   +++L  +  +  +  +L EM++ G V KL  +L       TKEKA +++KLH  
Sbjct: 370 TASDRAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHAR 402

[Close]

BLAST of Lsi09G018730 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 182.6 bits (462), Expect = 7.2e-46
Identity = 130/453 (28.70%), Postives = 224/453 (49.45%), Query Frame = 0


Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           ++ P +F CPIS+++M+DPV +STG++++R +IEKW F+  K +CP T Q I   D+TPN
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPN 64

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEY-DDVVADLLKNIESSPF-KASSLKKLRLLVA 141
           +TL+RLI SW    +      +P+         +  L+K   SS   +   LK+LR +V+
Sbjct: 65  HTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIVS 124

Query: 142 MDDVAAVGAKPVFVRANGFEVLVGIVTQVV-----------------ICESSDFAN-FEA 201
            +       K     A   E L  IV+  V                 +C+S+   N F++
Sbjct: 125 ENTT----NKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 184

Query: 202 ----CEEALGVLSQFPFSKTEKPFELLSK--PEAIKSMAIVLQRGSIEGRFYAMEMLQQI 261
                +EAL VL     S+T     L +K     +K++  ++QRG  E R YA  +L+++
Sbjct: 185 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 244

Query: 262 SKKGYDWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALEL 321
            +       +      +LF  ++++  D+I  KA+  ++   +++I C +G         
Sbjct: 245 LEVADPMQIILLER--ELFGEVIQILHDQISHKAT--RSAMQILVITCPWG--------- 304

Query: 322 LIEIMESSKKSRLLSIEAGAVCVMVDLLPD----SNRSKCEKILHILRLLSECAEGRSAM 381
                    ++R  ++E G + ++++LL D    S R   E  + +L +L +CAEGR+  
Sbjct: 305 ---------RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEF 364

Query: 382 GEHIMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNG 441
             H   IA V+KK + +S   ++  +++L  +  +  +  +L+EM++ G V KL  +L  
Sbjct: 365 LNHGAAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQV 424

Query: 442 DGRPSTKEKAMKIMKLHGSFWRRFPCFPYEFRD 444
                TKEKA +++KLH   WR  PC P    D
Sbjct: 425 SCGNKTKEKAKELLKLHARVWRESPCVPRNLYD 431

[Close]

BLAST of Lsi09G018730 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 149.1 bits (375), Expect = 8.8e-36
Identity = 119/445 (26.74%), Postives = 212/445 (47.64%), Query Frame = 0


Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTI-VNFDITP 81
           ++ P YF CPIS+E+M+DPVT  +G++++R NI KW       +CP T Q + ++ D+TP
Sbjct: 22  IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTP 81

Query: 82  NYTLKRLILSWKMKESCS-----SSSLLPSSSMEYDDVVADLLKNIESSPFKASSLKKLR 141
           N+ L+RLI  W ++         S+  +P   +   + + +L K  + +  +  +L+KL 
Sbjct: 82  NHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLE 141

Query: 142 LLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEACEEALGVLSQFPFS 201
           +L AMD       + +       + L+  V +    +       +  +E+L +L      
Sbjct: 142 VL-AMDG----NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIP 201

Query: 202 KTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQISKKGYDWNSLTKHHAIDLFK 261
             +    L+     ++S+  VL +     + Y + +L+ +++  Y  + + +    ++FK
Sbjct: 202 SNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFK 261

Query: 262 SILELASDEI---------------------IKKASTIKTTDSLIIIQCRYGTLFSSALE 321
            I+    D +                     + K    K   SL+I Q       ++AL 
Sbjct: 262 GIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQA-----VTAALM 321

Query: 322 LLIEIMESSKKSRLLSIEAGAVCVMVDLLPDSNRSK--CEKILHILRLLSECAEGRSAMG 381
           +L+E   S  ++R L ++ GAV  +++L       K   E +L +L  L  CA GR+ + 
Sbjct: 322 ILLE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEIL 381

Query: 382 EHIMGIATVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGD 437
            H  GIA VTK+ + +S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D
Sbjct: 382 AHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD 441

[Close]

BLAST of Lsi09G018730 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-33
Identity = 121/439 (27.56%), Postives = 205/439 (46.70%), Query Frame = 0


Query: 11  LPQQPPPPAAIVDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATM 70
           +P+   P    +  P +FRCPIS+ELMQDPVT+ TG +++RA+IE W    N  TCP T 
Sbjct: 1   MPRNIEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTR 60

Query: 71  QTIVNFDITPNYTLKRLILSWKMKESCSSSSLLPSSSMEYDDV-VADLLKNIE------- 130
             + +F + PN+TL+RLI  W +    +    +P+     D   V  LL           
Sbjct: 61  APLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHV 120

Query: 131 SSPFKASSLKKLRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEAC 190
           S   +A++L++LR      D   V    +    N  E+L+ I+        S+  + E  
Sbjct: 121 SVRSRAAALRRLRGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELV 180

Query: 191 EEALGVLSQFPFSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQIS--KKGY 250
            E+L +L   P ++  +   + S P  ++ +  +L   SIE R  A  +++ +S   K  
Sbjct: 181 SESLALLVMLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSA 240

Query: 251 DWNSLTKHHAIDLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEIM 310
           D    +  ++  +F+ +L+L  + I  + +          ++    TLF+         +
Sbjct: 241 DLKG-SISNSESVFEGVLDLLRNPISSRRA----------LKIGIKTLFA---------L 300

Query: 311 ESSKKSRLLSIEAGAVCVMVD-LLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIA 370
            S K +R ++I AGA  +++D L  D +R   E+ L  + LL    EG +A GEH + + 
Sbjct: 301 CSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVP 360

Query: 371 TVTKK-MSISNTATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKE 430
            + K  + +S+ AT+     L  +C     ER  EE    G V +LL ++  +     K+
Sbjct: 361 LLVKTILRVSDRATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSECTERAKK 406

Query: 431 KAMKIMKLHGSFWRRFPCF 438
           KA K++KL    W  +  F
Sbjct: 421 KAQKLLKLLRDSWPDYNSF 406

[Close]

BLAST of Lsi09G018730 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 127.9 bits (320), Expect = 2.1e-29
Identity = 117/422 (27.73%), Postives = 195/422 (46.21%), Query Frame = 0


Query: 22  VDFPPYFRCPISMELMQDPVTISTGVSFERANIEKWFFTYNKKTCPATMQTIVNFDITPN 81
           +  P +FRCPIS++LM DPVTISTG +++R +I+ W    N  TCP T   + +F + PN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALSDFTLIPN 71

Query: 82  YTLKRLILSWKMKESCSSSSLLPSSSMEYDDV-VADLLKNIE-------SSPFKASSLKK 141
           +TL+RLI  W +    +    +P+     D + V  LL           S   +A+++++
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRR 131

Query: 142 LRLLVAMDDVAAVGAKPVFVRANGFEVLVGIVTQVVICESSDFANFEACEEALGVLSQFP 201
           LR L    +   V    +    N  E+LV I+   +  E++  ++ E   E+L +L    
Sbjct: 132 LRGLARDSEKNRV----LIAGHNAREILVRILFADI--ETTSLSS-ELVSESLALLVLLH 191

Query: 202 FSKTEKPFELLSKPEAIKSMAIVLQRGSIEGRFYAMEMLQQI--SKKGYDWNSLTKHHAI 261
            ++TE    + S P  +  M  +L   SIE R  A  +++ +    K  D   L    + 
Sbjct: 192 MTETECE-AVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDL-KLIISGSD 251

Query: 262 DLFKSILELASDEIIKKASTIKTTDSLIIIQCRYGTLFSSALELLIEIMESSKKSRLLSI 321
            +F+ +L+L  + I  + + +K     I   C                    K++R L+I
Sbjct: 252 SIFEGVLDLLKNPISSRRA-LKIGIKAIFALCLV------------------KQTRHLAI 311

Query: 322 EAGAVCVMVD-LLPDSNRSKCEKILHILRLLSECAEGRSAMGEHIMGIATVTKK-MSISN 381
            AGA  +++D L  D +R   E+ L  + LL    EG +A GEH + +  + K  + +S+
Sbjct: 312 SAGAPGILIDRLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSD 371

Query: 382 TATKIGMKILWLICNYHPSERVLEEMMECGTVKKLLGILNGDGRPSTKEKAMKIMKLHGS 432
            AT+     L  +C     ER  +E    G V +LL ++  D     K KA  ++KL   
Sbjct: 372 RATEYAAGALLALCT--AEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRD 402

The following BLAST results are available for this feature:

BLAST of Lsi09G018730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	5.7e-48	31.24	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	1.0e-44	28.70	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9SF15	1.2e-34	26.74	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	1.5e-32	27.56	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9FXA4	2.9e-28	27.73	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]

BLAST of Lsi09G018730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CC54	1.1e-230	91.35	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ...	[more]
A0A0A0KBP6	9.0e-230	92.05	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE...	[more]
A0A6J1H6I8	1.3e-215	86.28	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075...	[more]
A0A6J1KWQ8	4.5e-213	86.73	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P...	[more]
A0A5D3DLY2	2.0e-205	83.59	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Lsi09G018730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875803.1	1.9e-234	92.89	E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida]	[more]
XP_008460073.1	2.2e-230	91.35	PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo]	[more]
XP_004145072.2	1.9e-229	92.05	E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN46274.1 hypothetical pro...	[more]
XP_023549278.1	5.6e-218	87.83	E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo]	[more]
KAG6574816.1	1.8e-216	86.73	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lsi09G018730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	4.1e-49	31.24	plant U-box 23	[more]
AT3G52450.1	7.2e-46	28.70	plant U-box 22	[more]
AT3G11840.1	8.8e-36	26.74	plant U-box 24	[more]
AT3G19380.1	1.1e-33	27.56	plant U-box 25	[more]
AT1G49780.1	2.1e-29	27.73	plant U-box 26	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 27..91 e-value: 9.3E-24 score: 94.9
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 25..95 e-value: 9.3E-17 score: 61.0
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 23..98 score: 27.193447
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 133..429 e-value: 6.9E-11 score: 43.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 19..102 e-value: 2.4E-23 score: 83.9
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 24..449
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 24..449
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 28..71 e-value: 6.52719E-21 score: 83.437
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 19..96
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 180..424

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G018730.1	Lsi09G018730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity