Lsi09G018580 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G018580
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	PMEI domain-containing protein
Location	chr09: 27389296 .. 27389868 (+)
RNA-Seq Expression	Lsi09G018580
Synteny	Lsi09G018580

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCCCTTTCTCAATCCATTCTTCTCTTGTTGTTCTTGTTCATGTTCTTCTCCAATTTTTCCATCACTCAATCTTCCAACAACACATCTTCCCTCATATACAAAACTTGCAAAGCCAGCTCTGACCAAGACCCCAACATCTCCTTCAATTTCTGCCTAACATCTCTCGAACCCGCATCAACCAAGCACGACCACGGCGCCACCAATCTCCGCCGCCTCGGCCTCATTACCATCTACTTGATTCGACACAACATGAGCAGCACGTGCCACCATATCAAGAAGTTGCGAAGAAACAAAGGCCTTGGCGACCCGTTGGTTAAGTCGTGTTTGGCTGATTGTTTGGAGCTTTATTCCGATGCCATCCCGACGGTGAAGCAAGCAAGGAAGGATTACAAGGCCAGGCGATACGCCGACGCGAACTCGAAGATCAGCTCGGTTATGGATGATTGTTCAACATGTGAAGATGGATTCAAGGAGAAACAAGGGGTGATTTCACCATTTACTAATAGGAATCATAATGCCTTTGAATTGTCGGCTATTGCACTGTCCATTATTAATATGCTTGCTTGA

mRNA sequence

ATGTCTCCCCTTTCTCAATCCATTCTTCTCTTGTTGTTCTTGTTCATGTTCTTCTCCAATTTTTCCATCACTCAATCTTCCAACAACACATCTTCCCTCATATACAAAACTTGCAAAGCCAGCTCTGACCAAGACCCCAACATCTCCTTCAATTTCTGCCTAACATCTCTCGAACCCGCATCAACCAAGCACGACCACGGCGCCACCAATCTCCGCCGCCTCGGCCTCATTACCATCTACTTGATTCGACACAACATGAGCAGCACGTGCCACCATATCAAGAAGTTGCGAAGAAACAAAGGCCTTGGCGACCCGTTGGTTAAGTCGTGTTTGGCTGATTGTTTGGAGCTTTATTCCGATGCCATCCCGACGGTGAAGCAAGCAAGGAAGGATTACAAGGCCAGGCGATACGCCGACGCGAACTCGAAGATCAGCTCGGTTATGGATGATTGTTCAACATGTGAAGATGGATTCAAGGAGAAACAAGGGGTGATTTCACCATTTACTAATAGGAATCATAATGCCTTTGAATTGTCGGCTATTGCACTGTCCATTATTAATATGCTTGCTTGA

Coding sequence (CDS)

ATGTCTCCCCTTTCTCAATCCATTCTTCTCTTGTTGTTCTTGTTCATGTTCTTCTCCAATTTTTCCATCACTCAATCTTCCAACAACACATCTTCCCTCATATACAAAACTTGCAAAGCCAGCTCTGACCAAGACCCCAACATCTCCTTCAATTTCTGCCTAACATCTCTCGAACCCGCATCAACCAAGCACGACCACGGCGCCACCAATCTCCGCCGCCTCGGCCTCATTACCATCTACTTGATTCGACACAACATGAGCAGCACGTGCCACCATATCAAGAAGTTGCGAAGAAACAAAGGCCTTGGCGACCCGTTGGTTAAGTCGTGTTTGGCTGATTGTTTGGAGCTTTATTCCGATGCCATCCCGACGGTGAAGCAAGCAAGGAAGGATTACAAGGCCAGGCGATACGCCGACGCGAACTCGAAGATCAGCTCGGTTATGGATGATTGTTCAACATGTGAAGATGGATTCAAGGAGAAACAAGGGGTGATTTCACCATTTACTAATAGGAATCATAATGCCTTTGAATTGTCGGCTATTGCACTGTCCATTATTAATATGCTTGCTTGA

Protein sequence

MSPLSQSILLLLFLFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGATNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCLELYSDAIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSAIALSIINMLA

Homology

[Close]

BLAST of Lsi09G018580 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-24
Identity = 64/176 (36.36%), Postives = 108/176 (61.36%), Query Frame = 0


Query: 14  LFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGATNLRR 73
           L +FF N  +   +  ++ ++  TCK  + + PN++++FC+ SL      H     +L+ 
Sbjct: 7   LCIFFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSH---TADLQG 66

Query: 74  LGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCLELYSDAIPTVKQARKDYK 133
           LG+I+  L   + S     I ++ ++K   DP +K  L DC+ LY+DA  +V++A  D+K
Sbjct: 67  LGVISANLAIQHGSKIQTFIGRILKSK--VDPALKKYLNDCVGLYADAKSSVQEAIADFK 126

Query: 134 ARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSAIALSIINML 190
           ++ YA AN K+S+ +DD  TCEDGFKEK+G++SP T  N +  +L+AI+L+I  +L
Sbjct: 127 SKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKLL 177

[Close]

BLAST of Lsi09G018580 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 9.2e-24
Identity = 60/160 (37.50%), Postives = 100/160 (62.50%), Query Frame = 0


Query: 30  TSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGATNLRRLGLITIYLIRHNMSST 89
           ++ ++  TCK  + + PN++++FC+ SL      H   + +L+ LG+I+  L     S  
Sbjct: 14  SADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSH---SADLQGLGVISANLAIQQGSKI 73

Query: 90  CHHIKKLRRNKGLGDPLVKSCLADCLELYSDAIPTVKQARKDYKARRYADANSKISSVMD 149
              I ++ ++K   DP +K  L DC+ LY+DA  +V++A  D+K++ YA AN K+S+ +D
Sbjct: 74  QTFIGRILKSK--VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALD 133

Query: 150 DCSTCEDGFKEKQGVISPFTNRNHNAFELSAIALSIINML 190
           D  TCEDGFKEK+G+ SP T  N +  +L+AI+L+I  +L
Sbjct: 134 DSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKLL 168

[Close]

BLAST of Lsi09G018580 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-09
Identity = 58/190 (30.53%), Postives = 86/190 (45.26%), Query Frame = 0


Query: 8   ILLLLFLFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHG 67
           ++ L+   +F + F+  Q      +LI  +CK +  +DP +S++FC+ SL          
Sbjct: 4   LVSLVIFSLFLNGFATAQ------TLIQDSCKKAFAKDPQLSYDFCVNSL--TQDPQSKA 63

Query: 68  ATNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKG-----LGDPLVKSCLAD---CLELYS 127
           AT L  L L          +ST     K+   KG     L D   +  + D   CL  Y+
Sbjct: 64  ATTLESLVL----------ASTKTAAAKITNLKGIVAQDLKDQRYQDIVEDLKLCLGFYN 123

Query: 128 DAIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELS 187
           DA   +  A  + K+R Y  AN  +S+ +D    CED FKE +   SP TN N   F+  
Sbjct: 124 DANDDLTTALANIKSRDYQGANINLSAALDVPGNCEDDFKEAKKT-SPITNENSILFKTI 174

Query: 188 AIALSIINML 190
            I L+  NML
Sbjct: 184 LIPLAFTNML 174

[Close]

BLAST of Lsi09G018580 vs. ExPASy TrEMBL
Match: A0A0A0KBR8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080370 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.9e-80
Identity = 163/192 (84.90%), Postives = 171/192 (89.06%), Query Frame = 0


Query: 1   MSPLSQSILLLLFLFMFFSNFSITQSS--NNTSSLIYKTCKASSDQDPNISFNFCLTSLE 60
           MS LS SIL  LFLFMFFSNF ITQSS  NNTSSLIYKTCKASS+QDPNISFNFC+TSL+
Sbjct: 1   MSSLSHSILPCLFLFMFFSNFPITQSSNNNNTSSLIYKTCKASSEQDPNISFNFCVTSLK 60

Query: 61  PASTKHDHGATNLRRLGLITIYLIRHNMSSTCHHIKK-LRRNKGLGDPLVKSCLADCLEL 120
           PA+TKH HG T+LRRLGLITIYLIRHNMS+T HHIKK L +NKG  DP VK CL DCLEL
Sbjct: 61  PAATKHRHGDTSLRRLGLITIYLIRHNMSNTRHHIKKYLHKNKGPRDPFVKLCLTDCLEL 120

Query: 121 YSDAIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFE 180
           YSDAIPTVKQARKDYKA RYADAN KISSVMDDCSTCE+GFKEK GVISP TNRNHNAFE
Sbjct: 121 YSDAIPTVKQARKDYKAGRYADANLKISSVMDDCSTCEEGFKEKDGVISPLTNRNHNAFE 180

Query: 181 LSAIALSIINML 190
           LSAIALSIINML
Sbjct: 181 LSAIALSIINML 192

[Close]

BLAST of Lsi09G018580 vs. ExPASy TrEMBL
Match: A0A1S3CBM6 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103498982 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.0e-78
Identity = 160/194 (82.47%), Postives = 173/194 (89.18%), Query Frame = 0


Query: 1   MSPLSQSILL-LLFLFM-FFSNFSITQSS---NNTSSLIYKTCKASSDQDPNISFNFCLT 60
           MS LS SIL+  LFLFM  FSNF ITQSS   NNTSSLIYKTCKASS+QDPNISF+FC+T
Sbjct: 1   MSSLSHSILIPCLFLFMLIFSNFPITQSSNNNNNTSSLIYKTCKASSEQDPNISFDFCIT 60

Query: 61  SLEPASTKHDHGATNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCL 120
           SL+PA+TKH HG T+LRRLGL+TIYLIRHNMSST HHIKKL+R KG GDP VK CL DCL
Sbjct: 61  SLKPAATKHRHGDTSLRRLGLMTIYLIRHNMSSTRHHIKKLQRKKGPGDPFVKLCLTDCL 120

Query: 121 ELYSDAIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNA 180
           ELYSDAIP VKQARKDYKA RYADANSKISSVMDDCSTCE+GF+EK+GV+SP TNRNHNA
Sbjct: 121 ELYSDAIPMVKQARKDYKAGRYADANSKISSVMDDCSTCEEGFEEKEGVVSPLTNRNHNA 180

Query: 181 FELSAIALSIINML 190
           FELSAIALSIINML
Sbjct: 181 FELSAIALSIINML 194

[Close]

BLAST of Lsi09G018580 vs. ExPASy TrEMBL
Match: A0A5A7TEK0 (Invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00910 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.2e-76
Identity = 148/177 (83.62%), Postives = 161/177 (90.96%), Query Frame = 0


Query: 16  MFFSNFSITQSS---NNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGATNLR 75
           + FSNF ITQSS   NNTSSLIYKTCKASS+QDPNISF+FC+TSL+PA+TKH HG T+LR
Sbjct: 2   LIFSNFPITQSSNNNNNTSSLIYKTCKASSEQDPNISFDFCITSLKPAATKHRHGDTSLR 61

Query: 76  RLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCLELYSDAIPTVKQARKDY 135
           RLGL+TIYLIRHNMSST HHIKKL+R KG GDP VK CL DCLELYSDAIP VKQARKDY
Sbjct: 62  RLGLMTIYLIRHNMSSTRHHIKKLQRKKGPGDPFVKLCLTDCLELYSDAIPMVKQARKDY 121

Query: 136 KARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSAIALSIINML 190
           KA RYADANSKISSVMDDCSTCE+GF+EK+GV+SP TNRNHNAFELSAIALSIINML
Sbjct: 122 KAGRYADANSKISSVMDDCSTCEEGFEEKEGVVSPLTNRNHNAFELSAIALSIINML 178

[Close]

BLAST of Lsi09G018580 vs. ExPASy TrEMBL
Match: E5GC99 (Invertase inhibitor OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.2e-76
Identity = 148/177 (83.62%), Postives = 161/177 (90.96%), Query Frame = 0


Query: 16  MFFSNFSITQSS---NNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGATNLR 75
           + FSNF ITQSS   NNTSSLIYKTCKASS+QDPNISF+FC+TSL+PA+TKH HG T+LR
Sbjct: 2   LIFSNFPITQSSNNNNNTSSLIYKTCKASSEQDPNISFDFCITSLKPAATKHRHGDTSLR 61

Query: 76  RLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCLELYSDAIPTVKQARKDY 135
           RLGL+TIYLIRHNMSST HHIKKL+R KG GDP VK CL DCLELYSDAIP VKQARKDY
Sbjct: 62  RLGLMTIYLIRHNMSSTRHHIKKLQRKKGPGDPFVKLCLTDCLELYSDAIPMVKQARKDY 121

Query: 136 KARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSAIALSIINML 190
           KA RYADANSKISSVMDDCSTCE+GF+EK+GV+SP TNRNHNAFELSAIALSIINML
Sbjct: 122 KAGRYADANSKISSVMDDCSTCEEGFEEKEGVVSPLTNRNHNAFELSAIALSIINML 178

[Close]

BLAST of Lsi09G018580 vs. ExPASy TrEMBL
Match: A0A6J1KIJ0 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494270 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 7.2e-64
Identity = 139/190 (73.16%), Postives = 158/190 (83.16%), Query Frame = 0


Query: 5   SQSILLLLFLFMFFSNFSITQSS-NNTSSLIYKTCKASSDQDPNISFNFCLTSLE---PA 64
           S S+ +L FLF  FSNF ITQSS +NTSSLIYKTCK SSDQDPNISFNFCLTSL+    A
Sbjct: 3   SPSLSILPFLFFLFSNFPITQSSISNTSSLIYKTCKTSSDQDPNISFNFCLTSLQHGVAA 62

Query: 65  STKHDHGATNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCLELYSD 124
           + KH   A +LRRLGLI++ L+R N+SST HHIKKL+RNK L + LVKSCLADCLELYSD
Sbjct: 63  TAKHGRTALDLRRLGLISLRLVRRNVSSTRHHIKKLQRNKHL-NTLVKSCLADCLELYSD 122

Query: 125 AIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSA 184
           AIP +K AR+DYK RRY + N KISSVMDDCSTCEDGF E++GVISP TNRN+N FELSA
Sbjct: 123 AIPILKDARRDYKDRRYFETNLKISSVMDDCSTCEDGF-EEEGVISPLTNRNYNVFELSA 182

Query: 185 IALSIINMLA 191
           IALSIINML+
Sbjct: 183 IALSIINMLS 190

[Close]

BLAST of Lsi09G018580 vs. NCBI nr
Match: XP_004145025.1 (putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 308.5 bits (789), Expect = 3.9e-80
Identity = 163/192 (84.90%), Postives = 171/192 (89.06%), Query Frame = 0


Query: 1   MSPLSQSILLLLFLFMFFSNFSITQSS--NNTSSLIYKTCKASSDQDPNISFNFCLTSLE 60
           MS LS SIL  LFLFMFFSNF ITQSS  NNTSSLIYKTCKASS+QDPNISFNFC+TSL+
Sbjct: 1   MSSLSHSILPCLFLFMFFSNFPITQSSNNNNTSSLIYKTCKASSEQDPNISFNFCVTSLK 60

Query: 61  PASTKHDHGATNLRRLGLITIYLIRHNMSSTCHHIKK-LRRNKGLGDPLVKSCLADCLEL 120
           PA+TKH HG T+LRRLGLITIYLIRHNMS+T HHIKK L +NKG  DP VK CL DCLEL
Sbjct: 61  PAATKHRHGDTSLRRLGLITIYLIRHNMSNTRHHIKKYLHKNKGPRDPFVKLCLTDCLEL 120

Query: 121 YSDAIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFE 180
           YSDAIPTVKQARKDYKA RYADAN KISSVMDDCSTCE+GFKEK GVISP TNRNHNAFE
Sbjct: 121 YSDAIPTVKQARKDYKAGRYADANLKISSVMDDCSTCEEGFKEKDGVISPLTNRNHNAFE 180

Query: 181 LSAIALSIINML 190
           LSAIALSIINML
Sbjct: 181 LSAIALSIINML 192

[Close]

BLAST of Lsi09G018580 vs. NCBI nr
Match: XP_038906889.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 303.5 bits (776), Expect = 1.3e-78
Identity = 160/190 (84.21%), Postives = 167/190 (87.89%), Query Frame = 0


Query: 1   MSPLSQSILLLLFLFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPA 60
           MS LS SIL  LFLFMFFSNF I +SSNNT SL+YKTCK  SDQDPNISFNFCLTSL PA
Sbjct: 1   MSSLSHSILRFLFLFMFFSNFLIIKSSNNTLSLLYKTCKTISDQDPNISFNFCLTSLVPA 60

Query: 61  STKHDHGATNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCLELYSD 120
           +TKH HGAT LRRLGLITIYLIR N+SST HHIK LRRNK  GDPLVKSCLADCLELYSD
Sbjct: 61  ATKHGHGATTLRRLGLITIYLIRRNVSSTRHHIKNLRRNKEPGDPLVKSCLADCLELYSD 120

Query: 121 AIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSA 180
           A  T+KQARKDYKARRY DA+S ISSVMDDCSTCEDGFKE +GVISP TNRNHNAFELSA
Sbjct: 121 AALTMKQARKDYKARRYDDASSMISSVMDDCSTCEDGFKE-EGVISPLTNRNHNAFELSA 180

Query: 181 IALSIINMLA 191
           IALSIINMLA
Sbjct: 181 IALSIINMLA 189

[Close]

BLAST of Lsi09G018580 vs. NCBI nr
Match: XP_008460050.2 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 302.8 bits (774), Expect = 2.2e-78
Identity = 160/194 (82.47%), Postives = 173/194 (89.18%), Query Frame = 0


Query: 1   MSPLSQSILL-LLFLFM-FFSNFSITQSS---NNTSSLIYKTCKASSDQDPNISFNFCLT 60
           MS LS SIL+  LFLFM  FSNF ITQSS   NNTSSLIYKTCKASS+QDPNISF+FC+T
Sbjct: 1   MSSLSHSILIPCLFLFMLIFSNFPITQSSNNNNNTSSLIYKTCKASSEQDPNISFDFCIT 60

Query: 61  SLEPASTKHDHGATNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCL 120
           SL+PA+TKH HG T+LRRLGL+TIYLIRHNMSST HHIKKL+R KG GDP VK CL DCL
Sbjct: 61  SLKPAATKHRHGDTSLRRLGLMTIYLIRHNMSSTRHHIKKLQRKKGPGDPFVKLCLTDCL 120

Query: 121 ELYSDAIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNA 180
           ELYSDAIP VKQARKDYKA RYADANSKISSVMDDCSTCE+GF+EK+GV+SP TNRNHNA
Sbjct: 121 ELYSDAIPMVKQARKDYKAGRYADANSKISSVMDDCSTCEEGFEEKEGVVSPLTNRNHNA 180

Query: 181 FELSAIALSIINML 190
           FELSAIALSIINML
Sbjct: 181 FELSAIALSIINML 194

[Close]

BLAST of Lsi09G018580 vs. NCBI nr
Match: ADN34098.1 (invertase inhibitor [Cucumis melo subsp. melo] >KAA0039957.1 invertase inhibitor [Cucumis melo var. makuwa] >TYK24546.1 invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 295.0 bits (754), Expect = 4.5e-76
Identity = 148/177 (83.62%), Postives = 161/177 (90.96%), Query Frame = 0


Query: 16  MFFSNFSITQSS---NNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGATNLR 75
           + FSNF ITQSS   NNTSSLIYKTCKASS+QDPNISF+FC+TSL+PA+TKH HG T+LR
Sbjct: 2   LIFSNFPITQSSNNNNNTSSLIYKTCKASSEQDPNISFDFCITSLKPAATKHRHGDTSLR 61

Query: 76  RLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLADCLELYSDAIPTVKQARKDY 135
           RLGL+TIYLIRHNMSST HHIKKL+R KG GDP VK CL DCLELYSDAIP VKQARKDY
Sbjct: 62  RLGLMTIYLIRHNMSSTRHHIKKLQRKKGPGDPFVKLCLTDCLELYSDAIPMVKQARKDY 121

Query: 136 KARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSAIALSIINML 190
           KA RYADANSKISSVMDDCSTCE+GF+EK+GV+SP TNRNHNAFELSAIALSIINML
Sbjct: 122 KAGRYADANSKISSVMDDCSTCEEGFEEKEGVVSPLTNRNHNAFELSAIALSIINML 178

[Close]

BLAST of Lsi09G018580 vs. NCBI nr
Match: KAE8646717.1 (hypothetical protein Csa_005489 [Cucumis sativus])

HSP 1 Score: 263.8 bits (673), Expect = 1.1e-66
Identity = 141/172 (81.98%), Postives = 149/172 (86.63%), Query Frame = 0


Query: 1   MSPLSQSILLLLFLFMFFSNFSITQSS--NNTSSLIYKTCKASSDQDPNISFNFCLTSLE 60
           MS LS SIL  LFLFMFFSNF ITQSS  NNTSSLIYKTCKASS+QDPNISFNFC+TSL+
Sbjct: 1   MSSLSHSILPCLFLFMFFSNFPITQSSNNNNTSSLIYKTCKASSEQDPNISFNFCVTSLK 60

Query: 61  PASTKHDHGATNLRRLGLITIYLIRHNMSSTCHHIKK-LRRNKGLGDPLVKSCLADCLEL 120
           PA+TKH HG T+LRRLGLITIYLIRHNMS+T HHIKK L +NKG  DP VK CL DCLEL
Sbjct: 61  PAATKHRHGDTSLRRLGLITIYLIRHNMSNTRHHIKKYLHKNKGPRDPFVKLCLTDCLEL 120

Query: 121 YSDAIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQG-VISPF 169
           YSDAIPTVKQARKDYKA RYADAN KISSVMDDCSTCE+GFKEK G   SPF
Sbjct: 121 YSDAIPTVKQARKDYKAGRYADANLKISSVMDDCSTCEEGFKEKDGPKFSPF 172

[Close]

BLAST of Lsi09G018580 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.9e-17
Identity = 60/186 (32.26%), Postives = 91/186 (48.92%), Query Frame = 0


Query: 13  FLFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGATNLR 72
           FL    + F ++    N  +LI  +CK ++   P   +N C+TSLE     +    T   
Sbjct: 3   FLLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLE----TNPQAKTAKD 62

Query: 73  RLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKS---------CLADCLELYSDAIP 132
             GL+        M+ST + + K    KG  D ++K           L DCL+LY+DAI 
Sbjct: 63  LAGLV--------MASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIG 122

Query: 133 TVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSAIAL 190
           ++ +A    K+R Y    + +S+ MD  STCE GFKE++   SP T  N N +++  I L
Sbjct: 123 SLNEALAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERKAP-SPVTKENDNLYQMILIPL 175

[Close]

BLAST of Lsi09G018580 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 5.6e-16
Identity = 63/176 (35.80%), Postives = 88/176 (50.00%), Query Frame = 0


Query: 11  LLFLFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGATN 70
           + FLF F   F +  +     SLI   CK ++D++P I +NFC+ SLE         A +
Sbjct: 1   MTFLFYFAMFFLLLNAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLE--ENPQSKTARS 60

Query: 71  LRRLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVK---------SCLADCLELYSDA 130
           L RL ++          ST + + K    KG+ D ++K           L DCLELYSDA
Sbjct: 61  LDRLVML----------STKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDA 120

Query: 131 IPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKE-KQGVISPFTNRNHNAF 177
             ++K+A    K+R Y  AN  IS+ M    +CE GFKE K+ + SPFT  N   F
Sbjct: 121 TNSLKEALTIIKSRDYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of Lsi09G018580 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.1e-14
Identity = 59/189 (31.22%), Postives = 94/189 (49.74%), Query Frame = 0


Query: 9   LLLLFLFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHGA 68
           L L+  F+  + F+  Q      SLI  +CK ++ +DP + ++FC+ SLE    ++    
Sbjct: 5   LYLVMFFLLLNCFATAQ------SLIRDSCKKATTKDPKLKYDFCVKSLE----ENPQSK 64

Query: 69  TNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKS-------CLADCLELYSDA 128
           T     GL+ +        ST + + K    KG+ D ++K         L DCLELY++A
Sbjct: 65  TAKSLEGLVFV--------STKNAVSKTTSLKGMVDKILKEDKYEVERPLLDCLELYTEA 124

Query: 129 IPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGF-KEKQGVISPFTNRNHNAFELSA 188
           I ++ Q+    K+R Y  A   +S+ MD   +CE  F K K+ V SPFT  N   F +  
Sbjct: 125 IDSLNQSLDTVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVL 175

Query: 189 IALSIINML 190
           I ++  +ML
Sbjct: 185 IPIAFTSML 175

[Close]

BLAST of Lsi09G018580 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 9.2e-11
Identity = 58/190 (30.53%), Postives = 86/190 (45.26%), Query Frame = 0


Query: 8   ILLLLFLFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHG 67
           ++ L+   +F + F+  Q      +LI  +CK +  +DP +S++FC+ SL          
Sbjct: 4   LVSLVIFSLFLNGFATAQ------TLIQDSCKKAFAKDPQLSYDFCVNSL--TQDPQSKA 63

Query: 68  ATNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKG-----LGDPLVKSCLAD---CLELYS 127
           AT L  L L          +ST     K+   KG     L D   +  + D   CL  Y+
Sbjct: 64  ATTLESLVL----------ASTKTAAAKITNLKGIVAQDLKDQRYQDIVEDLKLCLGFYN 123

Query: 128 DAIPTVKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELS 187
           DA   +  A  + K+R Y  AN  +S+ +D    CED FKE +   SP TN N   F+  
Sbjct: 124 DANDDLTTALANIKSRDYQGANINLSAALDVPGNCEDDFKEAKKT-SPITNENSILFKTI 174

Query: 188 AIALSIINML 190
            I L+  NML
Sbjct: 184 LIPLAFTNML 174

[Close]

BLAST of Lsi09G018580 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.0e-10
Identity = 54/185 (29.19%), Postives = 90/185 (48.65%), Query Frame = 0


Query: 8   ILLLLFLFMFFSNFSITQSSNNTSSLIYKTCKASSDQDPNISFNFCLTSLEPASTKHDHG 67
           ++ L+   +F + F+  Q      +LI  +CK +  +DP  S++FC+ SL          
Sbjct: 4   LVSLVIFSLFLNGFATAQ------TLIQDSCKKAFAKDPQSSYDFCVQSL--TQDPQSKA 63

Query: 68  ATNLRRLGLITIYLIRHNMSSTCHHIKKLRRNKGLGDPLVKSCLAD---CLELYSDAIPT 127
           AT L  L L +      N+++   ++K +   + L D   +  + D   CL  Y DA  +
Sbjct: 64  ATTLEDLALAS----TKNVAAKITNLKGIVA-QDLKDQRYQDIVEDLKLCLGFYKDANDS 123

Query: 128 VKQARKDYKARRYADANSKISSVMDDCSTCEDGFKEKQGVISPFTNRNHNAFELSAIALS 187
           +K A  + K+R Y  ANS +S+ ++    CED FKE +   SP TN N+  ++   I L+
Sbjct: 124 LKTALANIKSRDYDGANSNLSAALNAPGDCEDDFKEAEKK-SPITNENNILYKTIVIPLA 174

Query: 188 IINML 190
              ML
Sbjct: 184 FTTML 174

The following BLAST results are available for this feature:

BLAST of Lsi09G018580 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	1.4e-24	36.36	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	9.2e-24	37.50	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	1.3e-09	30.53	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]

BLAST of Lsi09G018580 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBR8	1.9e-80	84.90	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080370 PE=4 S...	[more]
A0A1S3CBM6	1.0e-78	82.47	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103498982 PE=4 SV=1	[more]
A0A5A7TEK0	2.2e-76	83.62	Invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G...	[more]
E5GC99	2.2e-76	83.62	Invertase inhibitor OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A6J1KIJ0	7.2e-64	73.16	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494270 PE=4 SV...	[more]

BLAST of Lsi09G018580 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145025.1	3.9e-80	84.90	putative invertase inhibitor [Cucumis sativus]	[more]
XP_038906889.1	1.3e-78	84.21	putative invertase inhibitor [Benincasa hispida]	[more]
XP_008460050.2	2.2e-78	82.47	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
ADN34098.1	4.5e-76	83.62	invertase inhibitor [Cucumis melo subsp. melo] >KAA0039957.1 invertase inhibitor...	[more]
KAE8646717.1	1.1e-66	81.98	hypothetical protein Csa_005489 [Cucumis sativus]	[more]

BLAST of Lsi09G018580 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46940.1	2.9e-17	32.26	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	5.6e-16	35.80	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	3.1e-14	31.22	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	9.2e-11	30.53	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	2.0e-10	29.19	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 29..185 e-value: 6.8E-25 score: 98.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..185 e-value: 1.3E-24 score: 87.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 8..189 e-value: 6.7E-39 score: 131.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 28..189 e-value: 1.0E-33 score: 118.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..189
None	No IPR available	PANTHER	PTHR31707:SF314	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR	coord: 1..187
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 1..187
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 38..188 e-value: 7.65798E-40 score: 130.948

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G018580.1	Lsi09G018580.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity