Homology
BLAST of Lsi09G003370 vs. ExPASy Swiss-Prot
Match:
A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)
HSP 1 Score: 978.0 bits (2527), Expect = 5.9e-284
Identity = 495/752 (65.82%), Postives = 592/752 (78.72%), Query Frame = 0
Query: 9 LLLLLNFHGYVALVTEFP--------LSSLRTYIVHVNKPE-----TTDDLESWHRSFLP 68
L LL FH + A +E P SS + YI+HV PE ++DLESW+ SFLP
Sbjct: 9 LTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWYHSFLP 68
Query: 69 TSTENSEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDF 128
+ +SEEQP ++YSY+NV+ GFAA LT+E++ A+E+K+GF+SA +R+L+ TTHTP F
Sbjct: 69 PTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHTPKF 128
Query: 129 LGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNN 188
LGL + G WK+SNFGKGVIIGVLD GITP HPSF DVG+P PP KWKGRC+ N +ACNN
Sbjct: 129 LGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTACNN 188
Query: 189 KLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVG 248
KLIGAR+FNLA++A+ GK ++PIDEDGHGTHTASTAAG FV+ AE LGNA GTA G
Sbjct: 189 KLIGARAFNLAAEAMNGKKA---EAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAG 248
Query: 249 MAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLG-SPSAPFFADNIAIGAL 308
MAP AHLAIYKVCFGEDCP++DILAALDAAVEDGVDV+S+SLG S PFF D+ AIGA
Sbjct: 249 MAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAF 308
Query: 309 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLF 368
AA+QKGIFVSC+A NSGPFN+++ N APWILTV ASTIDRRIVA AKLGNG+EFDGES+F
Sbjct: 309 AAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGESVF 368
Query: 369 QPNDFPQTFLPLVFPGEK-NETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 428
QP+ F T LPL + G+ E A C GSL + +GKVV+CERGGGIARIAKG EVK
Sbjct: 369 QPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGEEVKR 428
Query: 429 AGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIG 488
AGGAAMIL+N E++ F+ AD H LPA+HVS+ ++IKAYINST P ATI+FKGT+IG
Sbjct: 429 AGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKGTVIG 488
Query: 489 DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSM 548
+ +PA+ASFSSRGP+L SPGILKPDI GPGV+ILAAWPFPL N+T++K TFN+ SGTSM
Sbjct: 489 NSLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIESGTSM 548
Query: 549 SCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHV 608
SCPHLSGIAAL+KSSHP WSPA IKSAIMT+A+ NL I+D+TL+P DLFA G+GHV
Sbjct: 549 SCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFATGSGHV 608
Query: 609 NPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFT 668
NPS+A DPGLVYDIQPDDYIPYLCGLGY +V +IAH+ I C + SIPEGELNYPSF+
Sbjct: 609 NPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKC--SASIPEGELNYPSFS 668
Query: 669 VELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRI 728
VELG +TFTRTVTNVG+ Y ++ AP V V V+P KL FS +NQK TYSVTF R
Sbjct: 669 VELGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSVTFSRT 728
Query: 729 GSISPSIEFAKGYLKWVSTKHVVGSPISFKFV 746
G + + E+A+G+LKWVSTKH V SPIS KF+
Sbjct: 729 GLGNKTQEYAQGFLKWVSTKHTVRSPISVKFI 755
BLAST of Lsi09G003370 vs. ExPASy Swiss-Prot
Match:
A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)
HSP 1 Score: 873.6 bits (2256), Expect = 1.6e-252
Identity = 452/742 (60.92%), Postives = 549/742 (73.99%), Query Frame = 0
Query: 9 LLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPE-----TTDDLESWHRSFLPTSTENSEE 68
+ LL+FH L T + L+TYIVHV+KP+ + DLES++ SFLP + SE
Sbjct: 9 IAFLLSFH----LTTAQRSTGLQTYIVHVDKPDAQVLANSADLESYYNSFLPATVSGSEV 68
Query: 69 QPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQFG 128
+++SY +V +GFAA+L+ E+VK ME+K GFVSA+ E++L LHTTHTP+FLGL + G
Sbjct: 69 PSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMG 128
Query: 129 FWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSA-CNNKLIGARS 188
FW++SN+GKGVIIG+LD GITP HPSF DV MP PPAKWKG+CEF +A CN K+IGAR+
Sbjct: 129 FWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARN 188
Query: 189 FNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHL 248
F S P DE+GHGTHTASTAAG FV+ A GNA GTAVGMAPLAH+
Sbjct: 189 FISGSGV----------PPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHI 248
Query: 249 AIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIF 308
A+YKVC + C D DILAALDAA++DGVDVLSLSLG S PF+ DNIAIGA AAI+KGIF
Sbjct: 249 AMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIF 308
Query: 309 VSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQT 368
VS SA N GP N+TLSNEAPWILTV AST DR+IVA A LGNG+++DGES FQP DFP T
Sbjct: 309 VSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHT 368
Query: 369 FLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILL 428
LPLV+PG +E A C GSL DVKGKVVVC+RGG +AR+ K VK+AGGAAMIL
Sbjct: 369 LLPLVYPGTSDEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMILA 428
Query: 429 NLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIAS 488
NLE DG T ADAHVLPA+HV + IK+YINST+ P A I+FKGTIIG SP+++S
Sbjct: 429 NLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKSSPSVSS 488
Query: 489 FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSGIA 548
FSSRGP+LASPGI+KPDI GPGV+ILAAWP ++N T T TFN++SGTSMSCPHLSGI
Sbjct: 489 FSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHLSGIV 548
Query: 549 ALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATDPG 608
AL+KS+HPDWSPA IKSAIMT+A+ +NLEG PILD+ PAD+FA GAGHVNPSKA+DPG
Sbjct: 549 ALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKASDPG 608
Query: 609 LVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELG-LPQT 668
L+YDIQ +DYI YLCGLGY+ + LI + + C SI E ELNYPSF++ LG Q
Sbjct: 609 LIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKC--ESSISEAELNYPSFSIILGPKTQN 668
Query: 669 FTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSIE 728
+TRTVTNVGD YT I V + V P L+F+ + Q+ATY+V+F + G +
Sbjct: 669 YTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFTQSGDFTD--R 728
Query: 729 FAKGYLKWVSTKHVVGSPISFK 744
F +G + W S ++VV SPIS K
Sbjct: 729 FVQGAISWSSNEYVVRSPISVK 732
BLAST of Lsi09G003370 vs. ExPASy Swiss-Prot
Match:
A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)
HSP 1 Score: 838.6 bits (2165), Expect = 5.6e-242
Identity = 452/759 (59.55%), Postives = 551/759 (72.60%), Query Frame = 0
Query: 9 LLLLLNFHGYVALVTEFPLS----------SLRTYIVHVNKPE------TTDDLESWHRS 68
L+LLL G +++++ P S +L TYIVHV K E +T++L +WH S
Sbjct: 10 LVLLL---GLISMLSFIPASIAAEEGQEHDNLTTYIVHVKKLEIEGPLQSTEELHTWHHS 69
Query: 69 FLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHT 128
FLP T N + +++SYRNV SGFA RLT E+ A++EK+ +S R ER L+LHTTHT
Sbjct: 70 FLP-ETSNKDR---MVFSYRNVASGFAVRLTPEEANALQEKEEVMSIRPERTLSLHTTHT 129
Query: 129 PDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEF-NFS 188
P FLGL + G W DSN GKGVIIGV+D GI P H SF+D GMP PPAKWKG CEF S
Sbjct: 130 PSFLGLRQGQGLWNDSNLGKGVIIGVIDTGIYPFHLSFNDEGMPPPPAKWKGHCEFTGGS 189
Query: 189 ACNNKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVG 248
CNNKLIGAR NL A++ + P ++ HGTHTA+ AAG FV+GA GNA G
Sbjct: 190 VCNNKLIGAR--NLVKSAIQ-------EPPYEDFFHGTHTAAEAAGRFVEGASVFGNARG 249
Query: 249 TAVGMAPLAHLAIYKVCFG---EDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADN 308
TA GMAP AHLAIYKVC ++CP++ ILAA+D A+EDGVDVLSLSLG S PFF D
Sbjct: 250 TAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMDIAIEDGVDVLSLSLGLGSLPFFEDP 309
Query: 309 IAIGALAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEF 368
IAIGA AA QKGIFVSCSAANSGP ++LSNEAPWILTV ASTIDR+I A+AKLGNG E+
Sbjct: 310 IAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPWILTVGASTIDRKISASAKLGNGAEY 369
Query: 369 DGESLFQPNDFPQTFLPLVF-PGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAK 428
+GE+LFQP DF LPLV+ EKN + ALC GSL+NI+VKGKVVVC+ GGGI IAK
Sbjct: 370 EGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPGSLRNINVKGKVVVCDLGGGIPFIAK 429
Query: 429 GVEVKNAGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVF 488
G EV +AGG+AMIL N+E+ GFTT A+AHVLPA HVS+ +L IKAYINST P AT++F
Sbjct: 430 GQEVLDAGGSAMILANIENFGFTTLANAHVLPAVHVSYAASLAIKAYINSTYTPTATVLF 489
Query: 489 KGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNL 548
+GTIIGD +P++A+FSSRGPS SPGILKPDI GPGV+ILAAW +DN F++
Sbjct: 490 QGTIIGDSLAPSVAAFSSRGPSQQSPGILKPDIIGPGVNILAAWAVSVDNKI---PAFDI 549
Query: 549 VSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFA 608
+SGTSMSCPHLSGIAAL+KS+HPDWSPA IKSAIMTTAN NL G PILDQ L+PAD+FA
Sbjct: 550 ISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSAIMTTANTLNLRGLPILDQRLQPADIFA 609
Query: 609 IGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGEL 668
GAGHVNP +A DPGLVYDIQP+DY+PYLCGLGY + +V++I + + C SI + EL
Sbjct: 610 TGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGYSDREVTIIVQRSVRCFNVKSIAQAEL 669
Query: 669 NYPSFTVELGL-PQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATY 728
NYPSF++ LG Q +TRT+TNVG YT I+ P A+ ++V P ++ F+ +NQK Y
Sbjct: 670 NYPSFSILLGSDSQFYTRTLTNVGPANSTYTVKIDVPLAMGISVSPSQITFTQVNQKVAY 729
Query: 729 SVTF-KRIGSISPSIEFAKGYLKWVSTKHVVGSPISFKF 745
V F +I + FA+G + WVS KHVV +PIS F
Sbjct: 730 FVDFIPQIKENRGNHTFAQGAITWVSDKHVVRTPISVIF 749
BLAST of Lsi09G003370 vs. ExPASy Swiss-Prot
Match:
A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)
HSP 1 Score: 811.2 bits (2094), Expect = 9.6e-234
Identity = 436/755 (57.75%), Postives = 537/755 (71.13%), Query Frame = 0
Query: 3 LLPSLFLLLLLNFHGYVALVTEF------PLSSLRTYIVHVNKPET--TDDLESWHRSFL 62
LL +L L+L ++ + EF S+L TYIVHV KP+ +DDL +++ S L
Sbjct: 9 LLLALILVLSVSLASAASQNLEFTELEDEDQSNLSTYIVHVRKPQVIQSDDLHTFYYSLL 68
Query: 63 PTSTENSEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPD 122
P ST+ + ++ ++++YRNV++GFA +LT E+ KA+++ + VSAR E+IL+LHTTHTP
Sbjct: 69 PESTKTTNQR--IVFTYRNVVNGFAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPS 128
Query: 123 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFS-AC 182
FLGL + G WK SN GKGVIIG+LD GI+P HPSF D GMP PPAKW G CEF C
Sbjct: 129 FLGLQQGLGLWKGSNSGKGVIIGILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTC 188
Query: 183 NNKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTA 242
NNK+IGAR+F +K K T+ P D+ GHGTHTASTAAG V GA GNA GTA
Sbjct: 189 NNKIIGARNF------VKTKNLTL---PFDDVGHGTHTASTAAGRPVQGANVYGNANGTA 248
Query: 243 VGMAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGA 302
VGMAP AH+A+YKVC C ++ ILA +D AV+DGVDVLSLSLG PS PFF D IA+GA
Sbjct: 249 VGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDDGVDVLSLSLGGPSGPFFEDPIALGA 308
Query: 303 LAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESL 362
AIQKGIFVSCSAANSGP ++LSNEAPWILTV AS+IDR I+A AKLGNG+E+ G+S+
Sbjct: 309 FGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVGASSIDRTIMATAKLGNGKEYVGQSV 368
Query: 363 FQPNDFPQTFLPLVFPGE--KNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEV 422
FQP DF + LPLV+ G N C SL DV+GKVV+CE GG + R+ KG V
Sbjct: 369 FQPKDFAPSLLPLVYAGANGNNNFSVFCAPESLNRSDVEGKVVLCEDGGFVPRVFKGKAV 428
Query: 423 KNAGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTI 482
K+AGGAAMIL+N + F AD HVLPA H+S+ L +K YINST+ P ATI+F+GT+
Sbjct: 429 KDAGGAAMILMNSVLEDFNPIADVHVLPAVHISYEAGLALKEYINSTSTPTATILFEGTV 488
Query: 483 IGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGT 542
IG+ +P + SFSSRGPS ASPGILKPDI GPG++ILAAWP LDN+T FN++SGT
Sbjct: 489 IGNLLAPQVTSFSSRGPSKASPGILKPDIIGPGLNILAAWPVSLDNSTT--PPFNIISGT 548
Query: 543 SMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAG 602
SMSCPHLSGIAAL+K+SHPDWSPA IKSAIMTTA+ NL G PILDQ L PAD+FA GAG
Sbjct: 549 SMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTASQVNLGGTPILDQRLVPADVFATGAG 608
Query: 603 HVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPS 662
HVNP KA DPGLVYDI+P+DYIPYLCGL Y + +V +I + + C I E ELNYPS
Sbjct: 609 HVNPVKANDPGLVYDIEPNDYIPYLCGLNYTDREVGVILQQRVRCSEVNHIAEAELNYPS 668
Query: 663 FTVELG-LPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTF 722
F++ LG Q +TRTV NVG YTA I P V +++ P +L F+ + QK TYSV+F
Sbjct: 669 FSILLGNTTQLYTRTVANVGPANSTYTAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSF 728
Query: 723 KRIGSISPSIEFAKGYLKWVSTKHVVGSPISFKFV 746
+ FA+G LKWVS K+ V SPISF F+
Sbjct: 729 IPFSEDRDNHTFAQGSLKWVSGKYSVRSPISFIFL 750
BLAST of Lsi09G003370 vs. ExPASy Swiss-Prot
Match:
G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)
HSP 1 Score: 800.0 bits (2065), Expect = 2.2e-230
Identity = 428/730 (58.63%), Postives = 517/730 (70.82%), Query Frame = 0
Query: 29 SLRTYIVHVNKPET-----TDDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARLT 88
+L TYIVHV K E ++DL SW+ SFLP + + E +++SYR V SGFA +LT
Sbjct: 34 NLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFPHKER---MVFSYRKVASGFAVKLT 93
Query: 89 EEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI 148
E+ K+++EK VSAR ER L LHTTHTP FLGL + G W D N GKGVIIG++D GI
Sbjct: 94 PEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTGI 153
Query: 149 TPNHPSFDDVGMPQPPAKWKGRCEF-NFSACNNKLIGARSFNLASKALKGKTTTMDDSPI 208
P HPSF+D GMP PPAKWKG CEF CNNKLIGAR NL A++ + P
Sbjct: 154 FPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGAR--NLVKSAIQ-------EPPF 213
Query: 209 DEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGE-DCPDTDILAA 268
+ HGTHTA+ AAG F++ A GNA G A GMAP AHLAIYKVC + C ++ ILAA
Sbjct: 214 ENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAA 273
Query: 269 LDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANSGPFNATLSNEA 328
+D A+EDGVDVLSLSLG S PFF D IAIGA AA Q G+FVSCSAANSGP +TLSNEA
Sbjct: 274 MDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEA 333
Query: 329 PWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFP-----GEKNETV 388
PWILTV ASTIDR+IVA+AKLGNGEE++GE+LFQP DF Q LPLV+P G + +
Sbjct: 334 PWILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQ 393
Query: 389 ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAH 448
+LC GSLKNID+ GKVV+C+ G ++ I KG EV N+GG AMIL N E+ GF+T A AH
Sbjct: 394 SLCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAH 453
Query: 449 VLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSRGPSLASPGIL 508
VLPA VS+ L IK+YI ST P AT++FKGTIIGD +P++ FSSRGPS SPGIL
Sbjct: 454 VLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGDSLAPSVVYFSSRGPSQESPGIL 513
Query: 509 KPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHPDWSPAV 568
KPDI GPGV+ILAAW +DN F++VSGTSMSCPHLSGIAALIKSSHPDWSPA
Sbjct: 514 KPDIIGPGVNILAAWAVSVDNKI---PAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAA 573
Query: 569 IKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATDPGLVYDIQPDDYIPYL 628
IKSAIMTTAN NL G PILDQ L PAD+FA GAGHVNP KA DPGLVYDI+P+DY+PYL
Sbjct: 574 IKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYL 633
Query: 629 CGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGL-PQTFTRTVTNVGDDCEV 688
CGLGY + ++ +I + C SIPE +LNYPSF++ LG Q +TRT+TNVG
Sbjct: 634 CGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANST 693
Query: 689 YTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTF-KRIGSISPSIEFAKGYLKWVSTKH 745
Y +E P A+ ++V P ++ F+ +N+K ++SV F +I + F +G L WVS +H
Sbjct: 694 YKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRH 747
BLAST of Lsi09G003370 vs. ExPASy TrEMBL
Match:
A0A5A7TJ55 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003250 PE=3 SV=1)
HSP 1 Score: 1332.4 bits (3447), Expect = 0.0e+00
Identity = 669/745 (89.80%), Postives = 690/745 (92.62%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
MVLLPSLFLLLLLNFHGY A VTE PLS+L TYIVHV KPE DDLE WHRSFLPTS +N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
EEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFFAD AIG+ AAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTE DAHVLPASHVSHT ALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN++SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPILD+TL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
TF+RTVT+VG VY VIEAP VSVTVRPRKL FS LNQKATYSVTFKRIGSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
EFA+GYLKWVS KHVV SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of Lsi09G003370 vs. ExPASy TrEMBL
Match:
A0A1S3ATZ7 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=1)
HSP 1 Score: 1327.0 bits (3433), Expect = 0.0e+00
Identity = 667/745 (89.53%), Postives = 688/745 (92.35%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
MVLLPSLFLLLLLNFHGY A VTE PLS+L TYIVHV KPE DDLE WHRSFLPTS +N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
EEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GM QPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFFAD AIG+ AAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTE DAHVLPASHVSHT ALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN++SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPILD+TL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
TF+RTVT+VG VY VIEAP VSVTVRPRKL FS LNQKATYSVTFKR GSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSI 720
Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
EFA+GYLKWVS KHVV SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of Lsi09G003370 vs. ExPASy TrEMBL
Match:
A0A0A0KKE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1)
HSP 1 Score: 1323.5 bits (3424), Expect = 0.0e+00
Identity = 666/745 (89.40%), Postives = 690/745 (92.62%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
MVLLPSLFLLLLLNFH Y A VTE P S+L TYIVHV KPE DDLESWHRSFLPTS EN
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
SEEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTH+P+FLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF D GMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
HLAIYKVCFGE C + DILA LDAAVEDGVDVLS+SLG P PFFAD AIGA AAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QTFLPLVFPGEKNETVALC EGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTEADAHVLPASHVSHT ALKIKAYINSTTYP ATIVFKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN+VSGTSMSCPHLSG
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPI+DQTL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
TF+RTVT VG EVY VIEAP VSVTVRPRK+IFS LNQKATYSVTFKRIGSISPS
Sbjct: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
EFA+GYLKWVS KH+V SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHLVRSPISVKFV 745
BLAST of Lsi09G003370 vs. ExPASy TrEMBL
Match:
A0A6J1H242 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE=3 SV=1)
HSP 1 Score: 1265.0 bits (3272), Expect = 0.0e+00
Identity = 639/745 (85.77%), Postives = 676/745 (90.74%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
MV PSLFLLLLLNFH ALVTE PL +L+TYIVHV KPETTDDLE WHRSFLP+S+
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSSSS- 60
Query: 61 SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
LLYSYRNVMSGFAARL+EEQVKAMEE DGFVSARRERIL LHTTHTPDFLGLNR
Sbjct: 61 ------LLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDDVGMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
RSFNLA+KALKG+ T MDDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
HLAIYKVCFGEDCPDTDILAALDAA+EDGVDVLSLSLGSPS PFF D +AIGA AAIQKG
Sbjct: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
T LPLV+ GEKN+T ALCGEGSLK+IDVKGKVVVCERGGGIARIAKG EVKNAGGAAMI
Sbjct: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDD-FSPA 480
LLN + DGF+TEADAHVLPASHVSH ALKIKAYINSTTYP ATI+FKGT+IGDD FSPA
Sbjct: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
Query: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLS 540
IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFN++SGTSMSCPHLS
Sbjct: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISGTSMSCPHLS 540
Query: 541 GIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKAT 600
GIAALIKSSHPDWSPA IKSAIMTTA+ITNLEG PI+D+ L+PADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLP 660
DPGLVYDIQPDDYIPYLCGLGYK+N+V+ IA KPI+CL PSIPEG+LNYPSFTV LG P
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
Query: 661 QTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPS 720
QTFTRTVTNVG EVYTAV+EAP ++SVT+RP K+ FS +NQK T+SVTFKRIGSISPS
Sbjct: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTFKRIGSISPS 720
Query: 721 IEFAKGYLKWVSTKHVVGSPISFKF 745
EF KGYLKWVS KHVV SPISFKF
Sbjct: 721 TEFGKGYLKWVSDKHVVRSPISFKF 737
BLAST of Lsi09G003370 vs. ExPASy TrEMBL
Match:
A0A6J1E0E3 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 PE=3 SV=1)
HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 619/756 (81.88%), Postives = 668/756 (88.36%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPE------TTDDLESWHRSFL 60
MV+LPSLFLL+L +FHG A T S+L+TYIVHV KPE +DLE+WHRSFL
Sbjct: 1 MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
Query: 61 PTSTENSEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPD 120
P+S SE+ P LLYS+ NVMSGFAARLTEE VKAMEEKDGF+SARRERIL LHTTHTP+
Sbjct: 61 PSS---SEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPN 120
Query: 121 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACN 180
FLGLNRQFGFWKDSNFGKG IIGVLDGGITPNHPSFDD GMP PP KWKGRCEFNFSACN
Sbjct: 121 FLGLNRQFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACN 180
Query: 181 NKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAV 240
NKLIGARSFNLA+KALKG+ T DSPIDEDGHGTHTASTAAG FVD AEALGNA GTAV
Sbjct: 181 NKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAV 240
Query: 241 GMAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGAL 300
GMAPLAHLAIYKVCFGEDCPD+DILAALDAAVEDGVDVLSLSLGS SAPFF D+IAIGA
Sbjct: 241 GMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAF 300
Query: 301 AAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLF 360
AA QKGIFVSCSA+NSGP NATLSNEAPWILTVAASTIDRRI+A AKLGNGEEFDGESLF
Sbjct: 301 AATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLF 360
Query: 361 QPNDFPQT-FLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 420
QP+DFP T FLPLV+ GEKNET ALCGEGSLK+IDVKGK VVCERGGGIARIAKG+EVKN
Sbjct: 361 QPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKN 420
Query: 421 AGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIG 480
AGGAAMILLN ++DGFTTEADAHVLPA+HV+H ALKIK YINST PIA I F+GT+IG
Sbjct: 421 AGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIG 480
Query: 481 -DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT-NTKSTFNLVSGT 540
DDFSPAIASFSSRGPSLASPGILKPDITGPG+SILAAWPFPLDNNT NTK TFN++SGT
Sbjct: 481 DDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNVISGT 540
Query: 541 SMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAG 600
SMSCPHLSGIAALIKSSHPDWSPA IKSAIMTTA+ITN +G PILDQ +PADLFA GAG
Sbjct: 541 SMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFATGAG 600
Query: 601 HVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPS 660
HVNPSKATDPGLVYDIQPDDYIPYLCGLGYK+N+VS+IAH+P+DC PSIPEGELNYPS
Sbjct: 601 HVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGELNYPS 660
Query: 661 FTVELGLPQTFTRTVTNVGDDCEVYTAVIEA-PAAVSVTVRPRKLIFSTLNQKATYSVTF 720
F+V LG PQTFTRTVTNVG EVYT +IEA P+ VSVTVRPRK+ FS +NQK YSVTF
Sbjct: 661 FSVALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAYSVTF 720
Query: 721 KRIGSIS-PSIEFAKGYLKWVSTKHVVGSPISFKFV 746
KRIGSI+ PS A+ YLK +S+KH+V SPIS KFV
Sbjct: 721 KRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKFV 750
BLAST of Lsi09G003370 vs. NCBI nr
Match:
XP_038876087.1 (LOW QUALITY PROTEIN: subtilisin-like protease 4 [Benincasa hispida])
HSP 1 Score: 1347.0 bits (3485), Expect = 0.0e+00
Identity = 670/716 (93.58%), Postives = 686/716 (95.81%), Query Frame = 0
Query: 30 LRTYIVHVNKPETTDDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQVKA 89
L TYIVHV KPET DDLESWHRSFLPTS ENSEEQP LLYSYRNVMSGFAARLTEEQVKA
Sbjct: 28 LHTYIVHVKKPETADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGFAARLTEEQVKA 87
Query: 90 MEEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPS 149
MEEKDGFVSARRE+IL+LHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPS
Sbjct: 88 MEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPS 147
Query: 150 FDDVGMPQPPAKWKGRCEFNFSACNNKLIGARSFNLASKALKGKTTTMDDSPIDEDGHGT 209
FDDVGMPQPP KWKGRCEFNFSACNNKLIGARSFNLASKALKG TTMDDSPIDEDGHGT
Sbjct: 148 FDDVGMPQPPTKWKGRCEFNFSACNNKLIGARSFNLASKALKGHATTMDDSPIDEDGHGT 207
Query: 210 HTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDG 269
HTASTAAGTF+DGAEALGNA+GTA GMAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDG
Sbjct: 208 HTASTAAGTFIDGAEALGNAIGTAXGMAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDG 267
Query: 270 VDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAA 329
VDVLSLSLGSPSAPFF D+IAIGA AIQKGIFV CSAANSGPFNATLSNEAPWILTVAA
Sbjct: 268 VDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLSNEAPWILTVAA 327
Query: 330 STIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETVALCGEGSLKNIDV 389
STIDRRIVAAAKLGNGEE DGESLFQPNDFPQTFLPLVFPGEKNETVALCGEGSLKNIDV
Sbjct: 328 STIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVFPGEKNETVALCGEGSLKNIDV 387
Query: 390 KGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAHVLPASHVSHTVAL 449
KGKVVVCERGGGIARIAKGVEVKNAGGAAMILLN ESDGFTTEADAHVLPASHVSHT AL
Sbjct: 388 KGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADAHVLPASHVSHTAAL 447
Query: 450 KIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILA 509
KIKAYINSTTYP ATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILA
Sbjct: 448 KIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILA 507
Query: 510 AWPFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITN 569
AWPFPLDNNTN K TFN+VSGTSMSCPHLSGIAALIKSSHPDWSPAVIKS+IMTTANITN
Sbjct: 508 AWPFPLDNNTNPKXTFNIVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSSIMTTANITN 567
Query: 570 LEGNPILDQTLEPADLFAIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLI 629
LEGNPI+DQTL+PADLFA GAGHVNPSKA DPGLVYDIQPDDYIPYLCGLGYKNNQVSL+
Sbjct: 568 LEGNPIVDQTLQPADLFATGAGHVNPSKANDPGLVYDIQPDDYIPYLCGLGYKNNQVSLV 627
Query: 630 AHKPIDCLTTPSIPEGELNYPSFTVELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVT 689
AHKP++CLTTPSIPEGELNYPSFTVELG PQTFTRTVTNVG EVYT V+EAPAAVSVT
Sbjct: 628 AHKPVECLTTPSIPEGELNYPSFTVELGPPQTFTRTVTNVGGGREVYTVVVEAPAAVSVT 687
Query: 690 VRPRKLIFSTLNQKATYSVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPISFKFV 746
V+PR LIFS LNQKATY+VTFKRIGSISPSIEFAKGY+KWVSTKHVV SPIS KFV
Sbjct: 688 VKPRTLIFSALNQKATYAVTFKRIGSISPSIEFAKGYIKWVSTKHVVRSPISIKFV 743
BLAST of Lsi09G003370 vs. NCBI nr
Match:
KAA0042818.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])
HSP 1 Score: 1332.4 bits (3447), Expect = 0.0e+00
Identity = 669/745 (89.80%), Postives = 690/745 (92.62%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
MVLLPSLFLLLLLNFHGY A VTE PLS+L TYIVHV KPE DDLE WHRSFLPTS +N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
EEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFFAD AIG+ AAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTE DAHVLPASHVSHT ALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN++SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPILD+TL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
TF+RTVT+VG VY VIEAP VSVTVRPRKL FS LNQKATYSVTFKRIGSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRIGSISPSI 720
Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
EFA+GYLKWVS KHVV SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of Lsi09G003370 vs. NCBI nr
Match:
XP_008437181.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])
HSP 1 Score: 1327.0 bits (3433), Expect = 0.0e+00
Identity = 667/745 (89.53%), Postives = 688/745 (92.35%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
MVLLPSLFLLLLLNFHGY A VTE PLS+L TYIVHV KPE DDLE WHRSFLPTS +N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
EEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GM QPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFFAD AIG+ AAIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTE DAHVLPASHVSHT ALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN++SGTSMSCPHLSG
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSG 540
Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPILD+TL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGAGHVNPSKAID 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYPSFMVKLGPVQ 660
Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
TF+RTVT+VG VY VIEAP VSVTVRPRKL FS LNQKATYSVTFKR GSISPSI
Sbjct: 661 TFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTFKRSGSISPSI 720
Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
EFA+GYLKWVS KHVV SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHVVRSPISVKFV 745
BLAST of Lsi09G003370 vs. NCBI nr
Match:
KGN50165.1 (hypothetical protein Csa_000664 [Cucumis sativus])
HSP 1 Score: 1323.5 bits (3424), Expect = 0.0e+00
Identity = 666/745 (89.40%), Postives = 690/745 (92.62%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
MVLLPSLFLLLLLNFH Y A VTE P S+L TYIVHV KPE DDLESWHRSFLPTS EN
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
SEEQP LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I++LHTTH+P+FLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF D GMPQPPAKWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
RS NLAS+ALKGK TT+DDSPIDEDGHGTHTASTAAGTFVDGAEALGNA GTAVGMAPLA
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
HLAIYKVCFGE C + DILA LDAAVEDGVDVLS+SLG P PFFAD AIGA AAIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QTFLPLVFPGEKNETVALC EGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTEADAHVLPASHVSHT ALKIKAYINSTTYP ATIVFKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLSG 540
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFN+VSGTSMSCPHLSG
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSGTSMSCPHLSG 540
Query: 541 IAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKATD 600
IAALIKS+HPDWSPA IKS+IMTTANITNLEGNPI+DQTL+PADLFAIGAGHVNPSKA D
Sbjct: 541 IAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGAGHVNPSKAVD 600
Query: 601 PGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLPQ 660
PGLVYDIQPDDYIPYLCGLGY NNQVSLIAHKPIDCLTT SIPEGELNYPSF V+LG Q
Sbjct: 601 PGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYPSFMVKLGQVQ 660
Query: 661 TFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSI 720
TF+RTVT VG EVY VIEAP VSVTVRPRK+IFS LNQKATYSVTFKRIGSISPS
Sbjct: 661 TFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTFKRIGSISPST 720
Query: 721 EFAKGYLKWVSTKHVVGSPISFKFV 746
EFA+GYLKWVS KH+V SPIS KFV
Sbjct: 721 EFAEGYLKWVSAKHLVRSPISVKFV 745
BLAST of Lsi09G003370 vs. NCBI nr
Match:
XP_023532974.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 640/745 (85.91%), Postives = 679/745 (91.14%), Query Frame = 0
Query: 1 MVLLPSLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNKPETTDDLESWHRSFLPTSTEN 60
MV PSLFLLLLLNFH ALVTE PL +L+TYIVHV KPETTDDLESWHRSFLP+S+
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVEKPETTDDLESWHRSFLPSSSS- 60
Query: 61 SEEQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR 120
LLYSYRNVMSGFAARL+EEQVKAMEEKDGFVSARRERIL LHTTHTPDFLGLNR
Sbjct: 61 ------LLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDDVGMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGKTTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLA 240
RSFNLA+K LKG+ T MDDSPIDEDGHGTHTASTAAG FV GAEALGNA GTAVGMAPLA
Sbjct: 181 RSFNLATKVLKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKG 300
HLAIYKVCFGEDCPDTDILAALDAA+EDGVDVLSLSLGSPS PFF D +AIGA AAIQKG
Sbjct: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
Query: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFP 360
IFVSCSAANSGPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEEFDGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
TFLPLV+ GEKN+T ALCGEGSLK+IDVKGK+VVCERGGGIARIAKG EVKNAGGAAMI
Sbjct: 361 PTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEVKNAGGAAMI 420
Query: 421 LLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDD-FSPA 480
LLN + DGF+TEADAHVLPASHVSH ALKIKAYINSTTYP ATI+FKGT+IGDD FSPA
Sbjct: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
Query: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNLVSGTSMSCPHLS 540
IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N+NTKSTFN++SGTSMSCPHLS
Sbjct: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNSNTKSTFNIISGTSMSCPHLS 540
Query: 541 GIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKAT 600
GIAALIKSSHPDWSPA IKSAIMTTA+ITNLEG PI+D+ L+PADLFA GAGHVNPSKA
Sbjct: 541 GIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGAGHVNPSKAA 600
Query: 601 DPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGELNYPSFTVELGLP 660
DPGLVYDIQPDDYIPYLCGLGYK+N+V+ IA KPI+CL PSIPEG+LNYPSFTV LG P
Sbjct: 601 DPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYPSFTVVLGPP 660
Query: 661 QTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPS 720
QTFTRTVTNVG EVYTAV+EAP ++SVT+RP K+ FS +N+K TYSVTFKRIGSISPS
Sbjct: 661 QTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINEKVTYSVTFKRIGSISPS 720
Query: 721 IEFAKGYLKWVSTKHVVGSPISFKF 745
EF KGYLKWVS KHVV SPISFKF
Sbjct: 721 TEFGKGYLKWVSDKHVVRSPISFKF 737
BLAST of Lsi09G003370 vs. TAIR 10
Match:
AT1G04110.1 (Subtilase family protein )
HSP 1 Score: 576.2 bits (1484), Expect = 3.7e-164
Identity = 336/751 (44.74%), Postives = 454/751 (60.45%), Query Frame = 0
Query: 31 RTYIVHVN-KPETTDDLES---WHRSFLPTS---TENSEEQP--MLLYSYRNVMSGFAAR 90
+TYIV ++ ET S WH SFL + E EE+P LLYSY + + GFAA+
Sbjct: 26 QTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQ 85
Query: 91 LTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLN--RQFGFWKDSNFGKGVIIGVL 150
LTE + + + V+ R + +L + TT++ FLGL+ G W S FG+G IIGVL
Sbjct: 86 LTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVL 145
Query: 151 DGGITPNHPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFNLASKALKGKTT 210
D G+ P PSFDD GMP P KWKG C+ F+ S+CN KLIGAR F +
Sbjct: 146 DTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSPEE 205
Query: 211 TMD-----DSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFG 270
+ + S D GHGTHTAST G+ V A LGN G A GMAP AH+A+YKVC+
Sbjct: 206 SPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVCWF 265
Query: 271 EDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANS 330
C +DILAA+D A++D VDVLSLSLG P + D IAIG A+++GI V C+A N+
Sbjct: 266 NGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAGNN 325
Query: 331 GPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQ----PNDFPQTFLPL 390
GP ++++N APW+ T+ A T+DRR A +L NG+ GESL+ N + +
Sbjct: 326 GPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEVIY 385
Query: 391 VFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLES 450
V G+K C GSL +++GK+V+C+RG R KG VK AGG AMIL N E
Sbjct: 386 VTGGDKGS--EFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEI 445
Query: 451 DGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSR 510
+ D H+LPA+ + +T ++ +KAY+N+T P A I+F GT+IG +P +A FS+R
Sbjct: 446 NQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSRAPEVAQFSAR 505
Query: 511 GPSLASPGILKPDITGPGVSILAAWP-------FPLDNNTNTKSTFNLVSGTSMSCPHLS 570
GPSLA+P ILKPD+ PGV+I+AAWP P D+ + F ++SGTSMSCPH+S
Sbjct: 506 GPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVS 565
Query: 571 GIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQTLEPADLFAIGAGHVNPSKAT 630
GI ALI+S++P+WSPA IKSA+MTTA++ + +G I D +PA +FAIGAGHVNP KA
Sbjct: 566 GITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAI 625
Query: 631 DPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCL-TTPSIPEGELNYPSFTVELGL 690
+PGLVY+IQP DYI YLC LG+ + + I HK + C P LNYPS V
Sbjct: 626 NPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKR 685
Query: 691 PQT---FTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTF--KRI 742
+T TR VTNVG +Y+ ++AP + V V P++L+F ++Q +Y V F K+
Sbjct: 686 GKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKK 745
BLAST of Lsi09G003370 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 568.5 bits (1464), Expect = 7.7e-162
Identity = 326/734 (44.41%), Postives = 450/734 (61.31%), Query Frame = 0
Query: 31 RTYIVHVN---KPETTDDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARL-TEEQ 90
+TYI+ VN KPE+ W+ TS NSE LLY+Y GF+A L + E
Sbjct: 28 KTYIIRVNHSDKPESFLTHHDWY-----TSQLNSESS--LLYTYTTSFHGFSAYLDSTEA 87
Query: 91 VKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPN 150
+ + + + + LHTT TP+FLGLN +FG + GVIIGVLD G+ P
Sbjct: 88 DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 147
Query: 151 HPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFNLASKALKG---KTTTMDD 210
SFDD MP+ P+KWKG CE F+ CN KLIGARSF+ + G +
Sbjct: 148 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 207
Query: 211 SPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGEDCPDTDIL 270
SP D DGHGTHT++TAAG+ V A LG A GTA GMA A +A YKVC+ C +DIL
Sbjct: 208 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 267
Query: 271 AALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQKGIFVSCSAANSGPFNATLSN 330
AA+D A+ DGVDVLSLSLG SAP++ D IAIGA +A+++G+FVSCSA NSGP A+++N
Sbjct: 268 AAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVAN 327
Query: 331 EAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETVALC 390
APW++TV A T+DR A A LGNG+ G SL+ L LV+ + + LC
Sbjct: 328 VAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLC 387
Query: 391 GEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAHVLP 450
GSL + V+GK+VVC+RG AR+ KG V++AGG MI+ N + G AD+H+LP
Sbjct: 388 LPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLP 447
Query: 451 ASHVSHTVALKIKAYINSTTYPIATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPD 510
A V ++ Y+ S + P A +VFKGT++ SP +A+FSSRGP+ +P ILKPD
Sbjct: 448 AIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPD 507
Query: 511 ITGPGVSILAAW-----PFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHPDWSP 570
+ GPGV+ILA W P LD ++ ++ FN++SGTSMSCPH+SG+A L+K++HP+WSP
Sbjct: 508 VIGPGVNILAGWSDAIGPTGLDKDSR-RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSP 567
Query: 571 AVIKSAIMTTANITNLEGNPILDQTLEP-ADLFAIGAGHVNPSKATDPGLVYDIQPDDYI 630
+ IKSA+MTTA + + P+ D ++ +A G+GHV+P KA PGLVYDI ++YI
Sbjct: 568 SAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYI 627
Query: 631 PYLCGLGYKNNQVSLIAHKP-IDCLTTPSIPEGELNYPSFTVELGLPQT--FTRTVTNVG 690
+LC L Y + + I +P ++C S P G+LNYPSF+V G + +TR VTNVG
Sbjct: 628 RFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGGKRVVRYTREVTNVG 687
Query: 691 DDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKRIGSISPSIEFAKGYLKWV 745
VY + +V ++V+P KL F ++ +K Y+VTF +S + + G + W
Sbjct: 688 AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWS 747
BLAST of Lsi09G003370 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 566.6 bits (1459), Expect = 2.9e-161
Identity = 333/759 (43.87%), Postives = 461/759 (60.74%), Query Frame = 0
Query: 6 SLFLLLLLNFHGYVALVTEFPLSSLRTYIVHVNK---PETTDDLESWHRSFLPTSTENSE 65
+ FLLL L F S TYIVH+ K P + D +W+ S L + ++++E
Sbjct: 10 AFFLLLCLGF-----CHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE 69
Query: 66 EQPMLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQF 125
LLY+Y N + GF+ RLT+E+ ++ + G +S E LHTT TP FLGL+
Sbjct: 70 ----LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHT 129
Query: 126 G-FWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRCE----FNFSACNNKL 185
+ ++ V++GVLD G+ P S+ D G P+ WKG CE F S CN KL
Sbjct: 130 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 189
Query: 186 IGARSFNLASKALKGKTTTMDD--SPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVG 245
IGAR F ++ G + SP D+DGHGTHT+STAAG+ V+GA LG A GTA G
Sbjct: 190 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 249
Query: 246 MAPLAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALA 305
MAP A +A+YKVC+ C +DILAA+D A+ D V+VLS+SLG + ++ D +AIGA A
Sbjct: 250 MAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFA 309
Query: 306 AIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQ 365
A+++GI VSCSA N+GP +++LSN APWI TV A T+DR A A LGNG+ F G SLF+
Sbjct: 310 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 369
Query: 366 PNDFPQTFLPLVFPGEKNETV--ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 425
P LP ++ G + LC G+L VKGK+V+C+RG AR+ KG VK
Sbjct: 370 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKA 429
Query: 426 AGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIG 485
AGG MIL N ++G ADAH+LPA+ V I+ Y+ + P A+I GT++G
Sbjct: 430 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 489
Query: 486 DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNLV 545
SP +A+FSSRGP+ +P ILKPD+ PGV+ILAAW P L +++ + FN++
Sbjct: 490 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR-RVEFNII 549
Query: 546 SGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILD-QTLEPADLFA 605
SGTSMSCPH+SG+AAL+KS HP+WSPA I+SA+MTTA T +G P+LD T +P+ F
Sbjct: 550 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 609
Query: 606 IGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSIPEGEL 665
GAGHV+P+ AT+PGL+YD+ +DY+ +LC L Y + Q+ ++ + C + S +L
Sbjct: 610 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADL 669
Query: 666 NYPSFTVEL-GL-PQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKLIFSTLNQKAT 725
NYPSF V + G+ +TRTVT+VG V V ++V P L F N+K +
Sbjct: 670 NYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKS 729
Query: 726 YSVTFKRIGSISPSIEFAKGYLKWVSTKHVVGSPISFKF 745
Y+VTF + S PS + G ++W KHVVGSP++ +
Sbjct: 730 YTVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756
BLAST of Lsi09G003370 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 562.4 bits (1448), Expect = 5.5e-160
Identity = 340/748 (45.45%), Postives = 448/748 (59.89%), Query Frame = 0
Query: 30 LRTYIVHV---NKPETTDDLESWHRSFLPTSTENSEEQPMLLYSYRNVMSGFAARLTEEQ 89
L +YIVHV +KP +WH S L S +S + LLYSY + GF+ARL+ Q
Sbjct: 30 LESYIVHVQRSHKPSLFSSHNNWHVSLL-RSLPSSPQPATLLYSYSRAVHGFSARLSPIQ 89
Query: 90 VKAMEEKDGFVSARRERILNLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPN 149
A+ +S ++ +HTTHTP FLG ++ G W +SN+G+ VI+GVLD GI P
Sbjct: 90 TAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPE 149
Query: 150 HPSFDDVGMPQPPAKWKGRCE----FNFSACNNKLIGARSFN----LASKALKGKTTTMD 209
HPSF D G+ P+ WKG CE F S+CN KLIGAR+F K
Sbjct: 150 HPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKES 209
Query: 210 DSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAPLAHLAIYKVCFGEDCPDTDI 269
SP D +GHGTHTASTAAG+ V A A GTA GMA A +A YK+C+ C D+DI
Sbjct: 210 RSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYDSDI 269
Query: 270 LAALDAAVEDGVDVLSLSLG-SPSAP-FFADNIAIGALAAIQKGIFVSCSAANSGPFNAT 329
LAA+D AV DGV V+SLS+G S SAP + D+IAIGA A + GI VSCSA NSGP T
Sbjct: 270 LAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPET 329
Query: 330 LSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPNDFPQTFLPLVFPGEKNETV 389
+N APWILTV AST+DR A A G+G+ F G SL+ P + L LV+ G+
Sbjct: 330 ATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCGS-- 389
Query: 390 ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNLESDGFTTEADAH 449
LC G L + V+GK+V+C+RGG AR+ KG VK AGGA MIL N G AD+H
Sbjct: 390 RLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAESGEELTADSH 449
Query: 450 VLPASHVSHTVALKIKAYINSTTYPIATIVFKGTIIG-DDFSPAIASFSSRGPSLASPGI 509
++PA+ V +I+ YI ++ P A I F GT+IG SP +A+FSSRGP+ +P I
Sbjct: 450 LVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVI 509
Query: 510 LKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNLVSGTSMSCPHLSGIAALIKSSHP 569
LKPD+ PGV+ILA W P LD + + FN++SGTSMSCPH+SG+AAL++ +HP
Sbjct: 510 LKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPHVSGLAALLRKAHP 569
Query: 570 DWSPAVIKSAIMTTANITNLEGNPILD-QTLEPADLFAIGAGHVNPSKATDPGLVYDIQP 629
DWSPA IKSA++TTA G PI D T + ++ F GAGHV+P+KA +PGLVYDI+
Sbjct: 570 DWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEV 629
Query: 630 DDYIPYLCGLGYKNNQVSLIAHKPI---DCLTTPSIPEGELNYPSFTV---ELGLPQTFT 689
+Y+ +LC +GY+ + + P C T+ G+LNYPSF+V G +
Sbjct: 630 KEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYK 689
Query: 690 RTVTNVGDDCE-VYTAVIEAPAAVSVTVRPRKLIFSTLNQKATYSVTFKR------IGSI 745
R V NVG + + VY +++PA V + V P KL FS Y VTFK +GS+
Sbjct: 690 RVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSV 749
BLAST of Lsi09G003370 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 556.6 bits (1433), Expect = 3.0e-158
Identity = 339/767 (44.20%), Postives = 450/767 (58.67%), Query Frame = 0
Query: 15 FHGYVALVTEFPLSSLR-----TYIVHVN---KPETTDDLESWHRSFLPTSTENSEEQPM 74
F+ + L P SS TYIVHV+ KP W+ S L + T + P
Sbjct: 5 FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS---PPS 64
Query: 75 LLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARRERILNLHTTHTPDFLGLNR--QFGF 134
++++Y V GF+ARLT + + + +S E++ +LHTT +P+FLGL + G
Sbjct: 65 IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGL 124
Query: 135 WKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPAKWKGRC----EFNFSACNNKLIGA 194
++S+FG ++IGV+D G+ P PSFDD G+ P KWKG+C +F SACN KL+GA
Sbjct: 125 LEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGA 184
Query: 195 RSFNLASKALKGK--TTTMDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAVGTAVGMAP 254
R F +A GK TT SP D DGHGTHTAS +AG +V A LG A G A GMAP
Sbjct: 185 RFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAP 244
Query: 255 LAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFADNIAIGALAAIQ 314
A LA YKVC+ C D+DILAA D AV DGVDV+SLS+G P++ D IAIGA AI
Sbjct: 245 KARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAID 304
Query: 315 KGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLF-QPN 374
+GIFVS SA N GP T++N APW+ TV A TIDR A KLGNG+ G S++ P
Sbjct: 305 RGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPG 364
Query: 375 DFPQTFLPLVFPGE----KNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 434
P PLV+ G + +LC EGSL VKGK+V+C+RG +R KG V+
Sbjct: 365 LDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRK 424
Query: 435 AGGAAMILLNLESDGFTTEADAHVLPASHVSHTVALKIKAYIN------STTYPIATIVF 494
GG MI+ N DG AD HVLPA+ V + +I+ YI+ S+ +P ATIVF
Sbjct: 425 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 484
Query: 495 KGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLD----NNTNTKS 554
KGT +G +P +ASFS+RGP+ +P ILKPD+ PG++ILAAWP + + N ++
Sbjct: 485 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 544
Query: 555 TFNLVSGTSMSCPHLSGIAALIKSSHPDWSPAVIKSAIMTTANITNLEGNPILDQ-TLEP 614
FN++SGTSM+CPH+SG+AAL+K++HPDWSPA I+SA++TTA + G P++D+ T
Sbjct: 545 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 604
Query: 615 ADLFAIGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKNNQVSLIAHKPIDCLTTPSI 674
+ + G+GHV+P+KA DPGLVYDI DYI +LC Y + I + DC
Sbjct: 605 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 664
Query: 675 PE-GELNYPSFTV------ELGLPQTFTRTVTNVGDDCEVYTAVIEAPAAVSVTVRPRKL 734
G LNYPSF+V E + F RTVTNVGD VY I P +VTV P KL
Sbjct: 665 GHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKL 724
Query: 735 IFSTLNQKATYSVTFKRIG-SISP-SIEFAKGYLKWVSTKHVVGSPI 741
F + QK ++ V K +SP + G++ W K V SP+
Sbjct: 725 SFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| A9QY38 | 5.9e-284 | 65.82 | Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1 | [more] |
| A0A0M3R8G2 | 1.6e-252 | 60.92 | Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1 | [more] |
| A9QY40 | 5.6e-242 | 59.55 | Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1 | [more] |
| A9QY39 | 9.6e-234 | 57.75 | Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1 | [more] |
| G7KEU7 | 2.2e-230 | 58.63 | Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5A7TJ55 | 0.0e+00 | 89.80 | Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3ATZ7 | 0.0e+00 | 89.53 | subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=... | [more] |
| A0A0A0KKE3 | 0.0e+00 | 89.40 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1 | [more] |
| A0A6J1H242 | 0.0e+00 | 85.77 | subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE... | [more] |
| A0A6J1E0E3 | 0.0e+00 | 81.88 | subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 P... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_038876087.1 | 0.0e+00 | 93.58 | LOW QUALITY PROTEIN: subtilisin-like protease 4 [Benincasa hispida] | [more] |
| KAA0042818.1 | 0.0e+00 | 89.80 | subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa] | [more] |
| XP_008437181.1 | 0.0e+00 | 89.53 | PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo] | [more] |
| KGN50165.1 | 0.0e+00 | 89.40 | hypothetical protein Csa_000664 [Cucumis sativus] | [more] |
| XP_023532974.1 | 0.0e+00 | 85.91 | subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo] | [more] |
