Lsi08G011560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G011560
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	chr08: 20191916 .. 20193713 (+)
RNA-Seq Expression	Lsi08G011560
Synteny	Lsi08G011560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGATCACGAGCTTCCTCCCCCAAGCCAACTTGTTCCTTTGGATTTCAACTCAATTCAAACTGTCCCTGACTCTCACTTGTGGCCGAACTCCAACGAGTTATCGCCTGCGATCGAGTCGGACGAAAGGGTTTCGCTCCCGAAAATCGACCTTCTCGACGATGGTGCGTCGGAGCTCATAGGGAAGGCCTGTGAGAAATGGGGAATGTTTCAATTGATTAACCACGGTGTCCTGAAAACCCTACTCGATGAAGCGGAAGAGGAGACTCGATGCCTTTTCGCTCTCCCGGCTAGACAGAAGATGAAGACATTACCGTCCACCTGCAGCCCCACCACCACCGGCTACGGCATGGCCGCAATATCGAAGTATCATCAGAAATTAATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCAGTTGATGATGCTAAGAAGCTTTGGCCAAATGACTACAAACGGTTTTGGTATACAATTTAGGGTTTATCGCCATAACATATTAATATATAAATTAAATTGTTAGGTAACTTTAATTTCATGCATATAAATTGGAGTTAAATTATCATAATATATAGTTTCACTCATTATAATTTGAATTTGGTTTAGAATTTTTCTAAATTTTAGTAGTCTTCATCCATTTTTCTCTATTTATTTAGAAAGATTTACGATATATATAACGATAAATTGAAACTCTAACCTTAAGTATCATCTTTTAGTTTACAAATGAAATCAAGTAAAACAAGCTATAAATCATTAACAAATGGTTTAAATTGTTTCACTCATAACATTTTAGGGTTTAAATATATACAGTAATTTAAAATTTTTAATTGATACCATCCTCAAAGTTTAGATATCTATAAATTAATTTTTCTCCTTTGATAAAAAATTAACTTCCTAATTTTAAGGTTTATTACTTATGAAAGTACATGAACCATTTAGAATTTTAACCCATTTTTAAAATAAAAGAGACTAAATTAATTGGAGACCCATATATCTCAAGAACACAAAATATAAATTTTGGTTTATAGTTTATGTTTCAAATTATCCATTTGTGTTTTGATTATAGAAATTTGTTGGTTTTATGGTATCTAATCACACTTTAAAGTAGTAAATAAATAAGTAAATCACACTAACAATTTTTATGTCATAATTGAAAATTTTCTACTAATTGAATTGTTACATTTTATTTTTTATTTTTTTGGTTTTAATTAGTGATGTAATGGAAGAGTATCAAAGCCAAATGAAGGGTTTAGGAGAAAGGCTAATTAGGTTAATCTTCAAGTTTCTTGACATCTCCGAGGAAAAGATGATGAAGCTGTTGACTTCAACCGGCGACTCCGGGGGGAAACCCTACATGGCCCTCCGCCTGAACTCGTATCCACCGTGCCCTGATCCCGATCAAGTCATGGGCCTCGCCGCCCACACCGACACCTCCCTCTTCACCATCCTTCATCAGGCACACAATGACGGGCTCCAGATCTTCAAAGACGAAGTAGGCTGGATTCCGTTGTCTCCGATGAGCGGTACTCTCGTCGTCAACGTCGGGGATATGCTACATATTCTGTCGAACGGGCGGTTACCGAGCATTCTTCACCGGGTGATGATACAAGAAAATAAGGAGCATAGATTTTCATTGGCATATTTCTATGATCCACCAGCTGATTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAAATTCCTCTTTATAAATGTGTGAATGTGAAAGAATATTTCACTATTAAGGCCAAGAAAGAGGGGAAAGGACTTTCTGCCATAAAAATATGA

mRNA sequence

ATGGCAGATCACGAGCTTCCTCCCCCAAGCCAACTTGTTCCTTTGGATTTCAACTCAATTCAAACTGTCCCTGACTCTCACTTGTGGCCGAACTCCAACGAGTTATCGCCTGCGATCGAGTCGGACGAAAGGGTTTCGCTCCCGAAAATCGACCTTCTCGACGATGGTGCGTCGGAGCTCATAGGGAAGGCCTGTGAGAAATGGGGAATGTTTCAATTGATTAACCACGGTGTCCTGAAAACCCTACTCGATGAAGCGGAAGAGGAGACTCGATGCCTTTTCGCTCTCCCGGCTAGACAGAAGATGAAGACATTACCGTCCACCTGCAGCCCCACCACCACCGGCTACGGCATGGCCGCAATATCGAAGTATCATCAGAAATTAATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCAGTTGATGATGCTAAGAAGCTTTGGCCAAATGACTACAAACGGTTTTGTGATGTAATGGAAGAGTATCAAAGCCAAATGAAGGGTTTAGGAGAAAGGCTAATTAGGTTAATCTTCAAGTTTCTTGACATCTCCGAGGAAAAGATGATGAAGCTGTTGACTTCAACCGGCGACTCCGGGGGGAAACCCTACATGGCCCTCCGCCTGAACTCGTATCCACCGTGCCCTGATCCCGATCAAGTCATGGGCCTCGCCGCCCACACCGACACCTCCCTCTTCACCATCCTTCATCAGGCACACAATGACGGGCTCCAGATCTTCAAAGACGAAGTAGGCTGGATTCCGTTGTCTCCGATGAGCGGTACTCTCGTCGTCAACGTCGGGGATATGCTACATATTCTGTCGAACGGGCGGTTACCGAGCATTCTTCACCGGGTGATGATACAAGAAAATAAGGAGCATAGATTTTCATTGGCATATTTCTATGATCCACCAGCTGATTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAAATTCCTCTTTATAAATGTGTGAATGTGAAAGAATATTTCACTATTAAGGCCAAGAAAGAGGGGAAAGGACTTTCTGCCATAAAAATATGA

Coding sequence (CDS)

ATGGCAGATCACGAGCTTCCTCCCCCAAGCCAACTTGTTCCTTTGGATTTCAACTCAATTCAAACTGTCCCTGACTCTCACTTGTGGCCGAACTCCAACGAGTTATCGCCTGCGATCGAGTCGGACGAAAGGGTTTCGCTCCCGAAAATCGACCTTCTCGACGATGGTGCGTCGGAGCTCATAGGGAAGGCCTGTGAGAAATGGGGAATGTTTCAATTGATTAACCACGGTGTCCTGAAAACCCTACTCGATGAAGCGGAAGAGGAGACTCGATGCCTTTTCGCTCTCCCGGCTAGACAGAAGATGAAGACATTACCGTCCACCTGCAGCCCCACCACCACCGGCTACGGCATGGCCGCAATATCGAAGTATCATCAGAAATTAATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCAGTTGATGATGCTAAGAAGCTTTGGCCAAATGACTACAAACGGTTTTGTGATGTAATGGAAGAGTATCAAAGCCAAATGAAGGGTTTAGGAGAAAGGCTAATTAGGTTAATCTTCAAGTTTCTTGACATCTCCGAGGAAAAGATGATGAAGCTGTTGACTTCAACCGGCGACTCCGGGGGGAAACCCTACATGGCCCTCCGCCTGAACTCGTATCCACCGTGCCCTGATCCCGATCAAGTCATGGGCCTCGCCGCCCACACCGACACCTCCCTCTTCACCATCCTTCATCAGGCACACAATGACGGGCTCCAGATCTTCAAAGACGAAGTAGGCTGGATTCCGTTGTCTCCGATGAGCGGTACTCTCGTCGTCAACGTCGGGGATATGCTACATATTCTGTCGAACGGGCGGTTACCGAGCATTCTTCACCGGGTGATGATACAAGAAAATAAGGAGCATAGATTTTCATTGGCATATTTCTATGATCCACCAGCTGATTTATACATTTCACCTTATTGTAAGCCATTGAGTGAATCTCCACAAATTCCTCTTTATAAATGTGTGAATGTGAAAGAATATTTCACTATTAAGGCCAAGAAAGAGGGGAAAGGACTTTCTGCCATAAAAATATGA

Protein sequence

MADHELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASELIGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAAISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHNDGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI

Homology

[Close]

BLAST of Lsi08G011560 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.2e-78
Identity = 151/340 (44.41%), Postives = 220/340 (64.71%), Query Frame = 0


Query: 16  DFNSIQTVPDSHLWPNSNEL----SPAIESDERVSLPKIDLLDDGASELIGKACEKWGMF 75
           DFNS+Q +P+S+ W + ++     S  I  +   ++P IDL D  AS+LIG AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  QLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPT-TTGYGMAAISKYHQKLMW 135
           Q++NHG+  +LL++ +   + LF+LP+ QK K   +T SP   +GYG+A IS +  KLMW
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHK---ATRSPDGVSGYGIARISSFFPKLMW 140

Query: 136 HEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISEEKMMK 195
           +EGFTI+GSP+D  ++LWP DY RFCD++ +Y   MK L   L+ L+   L I++E +  
Sbjct: 141 YEGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDIK- 200

Query: 196 LLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHNDGLQIFKDEV 255
               +     K   AL+LNSYP CPDPD  MGLA HTD++  TIL Q    GLQ+ ++  
Sbjct: 201 -WAGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGS 260

Query: 256 GWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDPPADLYIS 315
           GWI + P+ G LVVNVGD+ HILSNG  PS+LHRV++   ++ RFS+AY Y PP+++ I 
Sbjct: 261 GWITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQ-RFSVAYLYGPPSNVEIC 320

Query: 316 PYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           P+ K +  + + PLY+ V   EY   KAK   K LS++++
Sbjct: 321 PHAKLIGPT-KPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

[Close]

BLAST of Lsi08G011560 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 283.5 bits (724), Expect = 3.3e-75
Identity = 150/337 (44.51%), Postives = 211/337 (62.61%), Query Frame = 0


Query: 14  PLDFNSIQTVPDSHLW-PNSNELSPAIESDERVSLPKIDLLDDGASELIGKACEKWGMFQ 73
           P DF S+   PDS+ W P  + L  A  SDE  +LP IDL D   + L+G AC  WG FQ
Sbjct: 21  PPDFKSL---PDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAAISKYHQKLMWHE 133
           + NHGV   LLD+ E  T  LF LP ++K+K   S      +GYG+A I+ +  K MW E
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARS--ENGVSGYGVARIASFFNKKMWSE 140

Query: 134 GFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISEEKMMKLL 193
           GFT++GSP+ D +KLWP+ + ++C+++EEY+  M+ L  +L+      L + EEK ++  
Sbjct: 141 GFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGV-EEKDIQWA 200

Query: 194 TSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHNDGLQIFKDEVGW 253
               D  G     ++LN YP CP+PD+ MGLAAHTD++L TIL+Q +  GLQ+F+D+VGW
Sbjct: 201 GPNSDFQG-TQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGW 260

Query: 254 IPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDPPADLYISPY 313
           +   P+ G+LVVNVGD+LHIL+NG  PS+LHR  +   +  RFS+AY + PP+D+ ISP 
Sbjct: 261 VTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPL 320

Query: 314 CKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIK 350
            K L +  Q PLY  +  K+Y   KA    + LS I+
Sbjct: 321 PK-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Lsi08G011560 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 279.6 bits (714), Expect = 4.7e-74
Identity = 150/349 (42.98%), Postives = 212/349 (60.74%), Query Frame = 0


Query: 4   HELPPPSQLVPLDFNSIQTVPDSHLW-PNSNELSPAIESDERV--SLPKIDLLDDGASEL 63
           H +  P   +P DF S++ +PDS+ W P  + L  A  S      ++P IDL    A+  
Sbjct: 12  HPIHLPHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQ 71

Query: 64  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 123
           IG AC  WG FQ+ NHGV   LL + E  T  LF LP ++K+K+  S      +GYG+A 
Sbjct: 72  IGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETG--VSGYGVAR 131

Query: 124 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 183
           I+ +  K MW EGFTI GSP++D +KLWP  +  +CD++EEY+  MK L  +L+ L    
Sbjct: 132 IASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNS 191

Query: 184 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 243
           L +SEE +     S+  +  +   AL+LN YP CP+PD+ MGLAAHTD++L TIL+Q + 
Sbjct: 192 LGVSEEDIEWASLSSDLNWAQ--AALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNT 251

Query: 244 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 303
            GLQ+F+D++GW+ + P  G+LVVNVGD+ HILSNG   S+LHR  + + +  R S+A+ 
Sbjct: 252 AGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFL 311

Query: 304 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIK 350
           + P +D+ ISP  K L    + PLY+ V  KEY   KA    K LS I+
Sbjct: 312 WGPQSDIKISPVPK-LVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Lsi08G011560 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.1e-65
Identity = 136/348 (39.08%), Postives = 208/348 (59.77%), Query Frame = 0


Query: 11  QLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASELIGKACEKWGM 70
           Q +P  F    T  DS L P+S     A+E     S+P IDL +   + LIG A + WG 
Sbjct: 17  QTLPESFTWKLTAADSLLRPSSAVSFDAVEE----SIPVIDLSNPDVTTLIGDASKTWGA 76

Query: 71  FQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAAISKYHQKLMW 130
           FQ+ NHG+ + LLD+ E  ++ LF +P+ +K++   S      +GYG   IS + +K MW
Sbjct: 77  FQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASS--DKGVSGYGEPRISPFFEKKMW 136

Query: 131 HEGFTILGSPVDDAKK-----LWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISE 190
            EGFTI     DD+ +     LWP+D+ ++C +++EY  +M+ L  RL+  I   L ++ 
Sbjct: 137 SEGFTI----ADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTV 196

Query: 191 E--KMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHNDGL 250
           E  +    L  +G   G+   A+RLN YP CP+P++ MGLAAHTD+++ TILHQ++  GL
Sbjct: 197 EDIEWAHKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGL 256

Query: 251 QIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDP 310
           Q+F++E GW+ + P  G LVVN+GD+ HILSNG++PS++HR  +   +  R S+AY +  
Sbjct: 257 QVFREESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGG 316

Query: 311 PA-DLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           PA D+ I+P  K L+   +  LY+ +  KEY  IK +   K + AI++
Sbjct: 317 PAGDVQIAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Lsi08G011560 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.9e-63
Identity = 136/344 (39.53%), Postives = 203/344 (59.01%), Query Frame = 0


Query: 11  QLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASELIGKACEKWGM 70
           +++PLDF + +T+PDSH+W   ++  P   S     +P I L +     L+ +ACE+WG+
Sbjct: 17  RIIPLDFTNTKTLPDSHVW---SKPEPETTSG---PIPVISLSNPEEHGLLRQACEEWGV 76

Query: 71  FQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAAISKYHQKLMW 130
           F + +HGV  +LL   + + + LF+LP  +K+  + S     +TGYG+  IS ++ KLMW
Sbjct: 77  FHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRS--PDESTGYGVVRISMFYDKLMW 136

Query: 131 HEGFTILGSPV-DDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISEEKMM 190
            EGF+++GS +   A  LWP+D+  FC+VMEEYQ  M  L  RLI ++   L ++ E + 
Sbjct: 137 SEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLG 196

Query: 191 KLLTSTGDSGGKPYMA-LRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHNDGLQI-FK 250
            L+     SG     + L+LNSYP CPDP   MGLA HTD+SL TIL+Q +  GL+I   
Sbjct: 197 WLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESP 256

Query: 251 DEVG--WIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDPPA 310
            E G  WI + P+ G+LVV +GD+ HI+SNG+  S +HR ++ +   HR S AYF  PP 
Sbjct: 257 QEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGPPK 316

Query: 311 DLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIK 350
           +L I P     S+    P+Y+ +  +EY   KA    K L+  +
Sbjct: 317 NLQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of Lsi08G011560 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.0e-180
Identity = 307/350 (87.71%), Postives = 327/350 (93.43%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASEL 60
           MAD ELPPPSQLVPLDFNSIQTVP+SHLWPNS ELSPAI+ DER+SLP ID LDDGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 120
           +G+ACE+WGMFQLINHGV KTL+DEAEEETR LF+LPARQKMKTL  T S T TGYGMA 
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTL-RTDSLTATGYGMAG 120

Query: 121 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 180
           ISKYHQKLMWHEGFT+ GSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGER+IRLIFKF
Sbjct: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKF 180

Query: 181 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 240
           LDISEE+MMKLLTST +S GKPYMALRLNSYPPCPDP +VMGLAAHTDTSLFTI+HQA +
Sbjct: 181 LDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARH 240

Query: 241 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 300
           DGLQ+FKD  GW+PLSPMSGTL+VNVGD+LHILSNGR PSILHRVMI ENKEHRFSLAYF
Sbjct: 241 DGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYF 300

Query: 301 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           YDPP DLYISPYCKPLSESPQIPLY+CVNVKEYFTIKAKKEGKGLSAIKI
Sbjct: 301 YDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of Lsi08G011560 vs. ExPASy TrEMBL
Match: S0BE47 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.6e-168
Identity = 283/350 (80.86%), Postives = 314/350 (89.71%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASEL 60
           MAD E+PPPSQLVPLDF S+QTVP+SHLWPN+ ELS AI+ D+RVSLP IDL+ DGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 120
           +G+ACE+WGMFQLINHG+ KTL++EAEEETR LF LPA QKMKTL S  S T TGYGMA 
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 180
           ISKYH KLMWHEGFTILGSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGER+IRLI KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 240
           L ISEE+MMKLLTST +S GKP+MALRLNSYPPCPDP QVMGLAAHTDTSL TILHQ  N
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 300
           +GLQIFKD  GW+PLSPMSGTL+VN+GD+LHILSNGR PSILHRVMIQENKEHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           YDPP ++YISPYCKPLS++PQ PLY+ VNVKEYF IKAKK GKGL AIKI
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of Lsi08G011560 vs. ExPASy TrEMBL
Match: A0A1S3BBH9 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 5.1e-164
Identity = 281/351 (80.06%), Postives = 311/351 (88.60%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQT-VPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASE 60
           MAD E+PPPSQLVPL+F S+QT VP+SHLWPN  ELS A++ D+RVSLP IDL++D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LIGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMA 120
           L+G+ACE+WGMFQLINHGV +TL++EAEEETR LF LPA QKMKTL S  S T TGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 AISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFK 180
            ISKYH KLMWHEGFTI+GSP+++AKKLWPNDYKRFCDVMEEYQ QMKGLGER+IRLI K
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAH 240
           FL ISEE+MMKLLTST +S GKPYMALRLNSYPPCPDP QVMGLAAHTDTSL TILHQ  
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 NDGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAY 300
           ++GLQIFKD  GW+PLSPMSGTLVVNVGD+LHILSNGR  SILHRVMIQENKEHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           FYDPP D+YISPYCKPLS++PQ PLY+ VNVKEYF IKAKK G GL AIKI
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

[Close]

BLAST of Lsi08G011560 vs. ExPASy TrEMBL
Match: A0A6J1KKU0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496619 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 7.1e-150
Identity = 261/350 (74.57%), Postives = 293/350 (83.71%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASEL 60
           MAD E       +PLDFNS+QTVP+SHLWP+S ELS  I+ DE++SLP IDLLDDGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 120
           +G+ACE+WGMFQL NHG+ KTL+ E EEE R LF LPA++KMK L S  +   TGYG A 
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRS--ADRVTGYGTAV 120

Query: 121 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 180
           ISKYH K MWHEGFTILGSP +DAKKLWP+D KRFCDVMEEYQSQMKGL +RLIRLIFKF
Sbjct: 121 ISKYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKF 180

Query: 181 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 240
           LDISEE+ MKLL+STGDS GK Y AL+LNSYPPCPDPDQVMGLAAHTDTSLFTI+HQ  N
Sbjct: 181 LDISEEETMKLLSSTGDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCN 240

Query: 241 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 300
           DGLQIFKD   W+PLSPM G+ VVNVGD+LHI+SNGR PSILHRV IQ  K HRFSLAYF
Sbjct: 241 DGLQIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYF 300

Query: 301 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           Y PP  LYISP+CKPLSESPQ PLY+CV+V+EYFT+KAK  G+GLS IKI
Sbjct: 301 YYPPPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

[Close]

BLAST of Lsi08G011560 vs. ExPASy TrEMBL
Match: A0A6J1GIY0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454608 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.7e-148
Identity = 257/350 (73.43%), Postives = 292/350 (83.43%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASEL 60
           MAD E       +PLDFNS+QTVP+SHLWP+S+ELS  I+ DE++SLP IDLLDDGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 120
           +G+ACE+WGMFQL NHG+ KTL+ E EEE R LF LP ++KMKTL S  +   TGYG A 
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRS--ADRVTGYGTAV 120

Query: 121 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 180
           ISKYH K MWHEGFTILGSP DDAKKLWP+D KRFCDVMEEYQS+MK L +RLI+LIFKF
Sbjct: 121 ISKYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKF 180

Query: 181 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 240
           LDIS E+ MKLL+S  DS GK Y AL+LNSYPPCPDPDQVMGLAAHTDTSLFTI+HQA N
Sbjct: 181 LDISVEETMKLLSSMDDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACN 240

Query: 241 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 300
           DGLQIFKD+ GW+PLSPM G+ VVNVGD+LHI+SNGR  SILHRV IQ  K HRFSLAYF
Sbjct: 241 DGLQIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYF 300

Query: 301 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           Y PP  LYISP+CKPLSESPQ PLY+CV+VKEYFT+KAK  G+GLS +KI
Sbjct: 301 YYPPPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

[Close]

BLAST of Lsi08G011560 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 642.5 bits (1656), Expect = 2.1e-180
Identity = 307/350 (87.71%), Postives = 327/350 (93.43%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASEL 60
           MAD ELPPPSQLVPLDFNSIQTVP+SHLWPNS ELSPAI+ DER+SLP ID LDDGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 120
           +G+ACE+WGMFQLINHGV KTL+DEAEEETR LF+LPARQKMKTL  T S T TGYGMA 
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTL-RTDSLTATGYGMAG 120

Query: 121 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 180
           ISKYHQKLMWHEGFT+ GSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGER+IRLIFKF
Sbjct: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKF 180

Query: 181 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 240
           LDISEE+MMKLLTST +S GKPYMALRLNSYPPCPDP +VMGLAAHTDTSLFTI+HQA +
Sbjct: 181 LDISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARH 240

Query: 241 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 300
           DGLQ+FKD  GW+PLSPMSGTL+VNVGD+LHILSNGR PSILHRVMI ENKEHRFSLAYF
Sbjct: 241 DGLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYF 300

Query: 301 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           YDPP DLYISPYCKPLSESPQIPLY+CVNVKEYFTIKAKKEGKGLSAIKI
Sbjct: 301 YDPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

[Close]

BLAST of Lsi08G011560 vs. NCBI nr
Match: XP_038885582.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-170
Identity = 291/352 (82.67%), Postives = 319/352 (90.62%), Query Frame = 0


Query: 1   MADH--ELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGAS 60
           MADH  ELPPPSQ VPLDF+SIQTVP+SHLWP+SNELS A+E DERVSLP IDLLD+GAS
Sbjct: 1   MADHDQELPPPSQFVPLDFSSIQTVPESHLWPDSNELSLAVELDERVSLPIIDLLDNGAS 60

Query: 61  ELIGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGM 120
           EL+G+ACE+WGMFQLINHGV KTL+DEAEEETR LF LPARQKMK L S  S   TGYGM
Sbjct: 61  ELLGRACEEWGMFQLINHGVPKTLIDEAEEETRRLFTLPARQKMKALQSPSS--ITGYGM 120

Query: 121 AAISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIF 180
           A ISKYH KLMWHEGFT+LGSPVDDAKKLWPNDYKRFCDVMEEYQ+QMKGLGERLIRLIF
Sbjct: 121 AGISKYHPKLMWHEGFTVLGSPVDDAKKLWPNDYKRFCDVMEEYQNQMKGLGERLIRLIF 180

Query: 181 KFLDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQA 240
           KFL IS+++M+K+LTST DSGGKPYMALRLNSYPPCPDP +V+GLAAHTDTSL TI+HQ 
Sbjct: 181 KFLHISDKEMVKMLTSTCDSGGKPYMALRLNSYPPCPDPSRVVGLAAHTDTSLLTIIHQV 240

Query: 241 HNDGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLA 300
            +DGLQ+F D  GW+PLSPMSGTL+VNVGD+LHILSNGR PSILHRV IQENKEHR+SL 
Sbjct: 241 RSDGLQVFNDGAGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVTIQENKEHRYSLG 300

Query: 301 YFYDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           YFYDPPA LYISPYCKPLSESPQIPLY+CVNV+EYFTIKA  +GKGLSAIKI
Sbjct: 301 YFYDPPAGLYISPYCKPLSESPQIPLYRCVNVQEYFTIKASNKGKGLSAIKI 350

[Close]

BLAST of Lsi08G011560 vs. NCBI nr
Match: NP_001275523.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetical protein Csa_021993 [Cucumis sativus] >CCG14217.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 601.3 bits (1549), Expect = 5.4e-168
Identity = 283/350 (80.86%), Postives = 314/350 (89.71%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASEL 60
           MAD E+PPPSQLVPLDF S+QTVP+SHLWPN+ ELS AI+ D+RVSLP IDL+ DGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 120
           +G+ACE+WGMFQLINHG+ KTL++EAEEETR LF LPA QKMKTL S  S T TGYGMA 
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 180
           ISKYH KLMWHEGFTILGSP+++AKKLWPNDYKRFCDVMEEYQ +MKGLGER+IRLI KF
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRFCDVMEEYQREMKGLGERIIRLILKF 180

Query: 181 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 240
           L ISEE+MMKLLTST +S GKP+MALRLNSYPPCPDP QVMGLAAHTDTSL TILHQ  N
Sbjct: 181 LGISEEEMMKLLTSTDESIGKPHMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVGN 240

Query: 241 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 300
           +GLQIFKD  GW+PLSPMSGTL+VN+GD+LHILSNGR PSILHRVMIQENKEHRFSLAYF
Sbjct: 241 NGLQIFKDGTGWVPLSPMSGTLIVNIGDLLHILSNGRFPSILHRVMIQENKEHRFSLAYF 300

Query: 301 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           YDPP ++YISPYCKPLS++PQ PLY+ VNVKEYF IKAKK GKGL AIKI
Sbjct: 301 YDPPGEIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKTGKGLPAIKI 350

[Close]

BLAST of Lsi08G011560 vs. NCBI nr
Match: XP_008445138.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 587.0 bits (1512), Expect = 1.1e-163
Identity = 281/351 (80.06%), Postives = 311/351 (88.60%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQT-VPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASE 60
           MAD E+PPPSQLVPL+F S+QT VP+SHLWPN  ELS A++ D+RVSLP IDL++D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LIGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMA 120
           L+G+ACE+WGMFQLINHGV +TL++EAEEETR LF LPA QKMKTL S  S T TGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 AISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFK 180
            ISKYH KLMWHEGFTI+GSP+++AKKLWPNDYKRFCDVMEEYQ QMKGLGER+IRLI K
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFCDVMEEYQRQMKGLGERIIRLILK 180

Query: 181 FLDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAH 240
           FL ISEE+MMKLLTST +S GKPYMALRLNSYPPCPDP QVMGLAAHTDTSL TILHQ  
Sbjct: 181 FLGISEEEMMKLLTSTDESIGKPYMALRLNSYPPCPDPGQVMGLAAHTDTSLCTILHQVR 240

Query: 241 NDGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAY 300
           ++GLQIFKD  GW+PLSPMSGTLVVNVGD+LHILSNGR  SILHRVMIQENKEHRFSLAY
Sbjct: 241 SNGLQIFKDGTGWVPLSPMSGTLVVNVGDLLHILSNGRFTSILHRVMIQENKEHRFSLAY 300

Query: 301 FYDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           FYDPP D+YISPYCKPLS++PQ PLY+ VNVKEYF IKAKK G GL AIKI
Sbjct: 301 FYDPPGDIYISPYCKPLSDTPQFPLYRSVNVKEYFAIKAKKMGSGLPAIKI 351

[Close]

BLAST of Lsi08G011560 vs. NCBI nr
Match: XP_023002882.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 540.0 bits (1390), Expect = 1.5e-149
Identity = 261/350 (74.57%), Postives = 293/350 (83.71%), Query Frame = 0


Query: 1   MADHELPPPSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASEL 60
           MAD E       +PLDFNS+QTVP+SHLWP+S ELS  I+ DE++SLP IDLLDDGASEL
Sbjct: 1   MADQE------FLPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 120
           +G+ACE+WGMFQL NHG+ KTL+ E EEE R LF LPA++KMK L S  +   TGYG A 
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRS--ADRVTGYGTAV 120

Query: 121 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 180
           ISKYH K MWHEGFTILGSP +DAKKLWP+D KRFCDVMEEYQSQMKGL +RLIRLIFKF
Sbjct: 121 ISKYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKF 180

Query: 181 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 240
           LDISEE+ MKLL+STGDS GK Y AL+LNSYPPCPDPDQVMGLAAHTDTSLFTI+HQ  N
Sbjct: 181 LDISEEETMKLLSSTGDS-GKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCN 240

Query: 241 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 300
           DGLQIFKD   W+PLSPM G+ VVNVGD+LHI+SNGR PSILHRV IQ  K HRFSLAYF
Sbjct: 241 DGLQIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYF 300

Query: 301 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           Y PP  LYISP+CKPLSESPQ PLY+CV+V+EYFT+KAK  G+GLS IKI
Sbjct: 301 YYPPPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

[Close]

BLAST of Lsi08G011560 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 283.5 bits (724), Expect = 2.3e-76
Identity = 150/337 (44.51%), Postives = 211/337 (62.61%), Query Frame = 0


Query: 14  PLDFNSIQTVPDSHLW-PNSNELSPAIESDERVSLPKIDLLDDGASELIGKACEKWGMFQ 73
           P DF S+   PDS+ W P  + L  A  SDE  +LP IDL D   + L+G AC  WG FQ
Sbjct: 21  PPDFKSL---PDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAAISKYHQKLMWHE 133
           + NHGV   LLD+ E  T  LF LP ++K+K   S      +GYG+A I+ +  K MW E
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARS--ENGVSGYGVARIASFFNKKMWSE 140

Query: 134 GFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISEEKMMKLL 193
           GFT++GSP+ D +KLWP+ + ++C+++EEY+  M+ L  +L+      L + EEK ++  
Sbjct: 141 GFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGV-EEKDIQWA 200

Query: 194 TSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHNDGLQIFKDEVGW 253
               D  G     ++LN YP CP+PD+ MGLAAHTD++L TIL+Q +  GLQ+F+D+VGW
Sbjct: 201 GPNSDFQG-TQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGW 260

Query: 254 IPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDPPADLYISPY 313
           +   P+ G+LVVNVGD+LHIL+NG  PS+LHR  +   +  RFS+AY + PP+D+ ISP 
Sbjct: 261 VTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRS-RFSMAYLWGPPSDIMISPL 320

Query: 314 CKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIK 350
            K L +  Q PLY  +  K+Y   KA    + LS I+
Sbjct: 321 PK-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of Lsi08G011560 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 279.6 bits (714), Expect = 3.3e-75
Identity = 150/349 (42.98%), Postives = 212/349 (60.74%), Query Frame = 0


Query: 4   HELPPPSQLVPLDFNSIQTVPDSHLW-PNSNELSPAIESDERV--SLPKIDLLDDGASEL 63
           H +  P   +P DF S++ +PDS+ W P  + L  A  S      ++P IDL    A+  
Sbjct: 12  HPIHLPHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQ 71

Query: 64  IGKACEKWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAA 123
           IG AC  WG FQ+ NHGV   LL + E  T  LF LP ++K+K+  S      +GYG+A 
Sbjct: 72  IGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETG--VSGYGVAR 131

Query: 124 ISKYHQKLMWHEGFTILGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKF 183
           I+ +  K MW EGFTI GSP++D +KLWP  +  +CD++EEY+  MK L  +L+ L    
Sbjct: 132 IASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNS 191

Query: 184 LDISEEKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN 243
           L +SEE +     S+  +  +   AL+LN YP CP+PD+ MGLAAHTD++L TIL+Q + 
Sbjct: 192 LGVSEEDIEWASLSSDLNWAQ--AALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNT 251

Query: 244 DGLQIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYF 303
            GLQ+F+D++GW+ + P  G+LVVNVGD+ HILSNG   S+LHR  + + +  R S+A+ 
Sbjct: 252 AGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFL 311

Query: 304 YDPPADLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIK 350
           + P +D+ ISP  K L    + PLY+ V  KEY   KA    K LS I+
Sbjct: 312 WGPQSDIKISPVPK-LVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of Lsi08G011560 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 251.9 bits (642), Expect = 7.5e-67
Identity = 136/348 (39.08%), Postives = 208/348 (59.77%), Query Frame = 0


Query: 11  QLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASELIGKACEKWGM 70
           Q +P  F    T  DS L P+S     A+E     S+P IDL +   + LIG A + WG 
Sbjct: 17  QTLPESFTWKLTAADSLLRPSSAVSFDAVEE----SIPVIDLSNPDVTTLIGDASKTWGA 76

Query: 71  FQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAAISKYHQKLMW 130
           FQ+ NHG+ + LLD+ E  ++ LF +P+ +K++   S      +GYG   IS + +K MW
Sbjct: 77  FQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASS--DKGVSGYGEPRISPFFEKKMW 136

Query: 131 HEGFTILGSPVDDAKK-----LWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISE 190
            EGFTI     DD+ +     LWP+D+ ++C +++EY  +M+ L  RL+  I   L ++ 
Sbjct: 137 SEGFTI----ADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTV 196

Query: 191 E--KMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHNDGL 250
           E  +    L  +G   G+   A+RLN YP CP+P++ MGLAAHTD+++ TILHQ++  GL
Sbjct: 197 EDIEWAHKLEKSGSKVGRG--AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGL 256

Query: 251 QIFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDP 310
           Q+F++E GW+ + P  G LVVN+GD+ HILSNG++PS++HR  +   +  R S+AY +  
Sbjct: 257 QVFREESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRS-RISIAYLWGG 316

Query: 311 PA-DLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIKI 351
           PA D+ I+P  K L+   +  LY+ +  KEY  IK +   K + AI++
Sbjct: 317 PAGDVQIAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Lsi08G011560 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 243.8 bits (621), Expect = 2.0e-64
Identity = 136/344 (39.53%), Postives = 203/344 (59.01%), Query Frame = 0


Query: 11  QLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASELIGKACEKWGM 70
           +++PLDF + +T+PDSH+W   ++  P   S     +P I L +     L+ +ACE+WG+
Sbjct: 17  RIIPLDFTNTKTLPDSHVW---SKPEPETTSG---PIPVISLSNPEEHGLLRQACEEWGV 76

Query: 71  FQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAAISKYHQKLMW 130
           F + +HGV  +LL   + + + LF+LP  +K+  + S     +TGYG+  IS ++ KLMW
Sbjct: 77  FHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRS--PDESTGYGVVRISMFYDKLMW 136

Query: 131 HEGFTILGSPV-DDAKKLWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISEEKMM 190
            EGF+++GS +   A  LWP+D+  FC+VMEEYQ  M  L  RLI ++   L ++ E + 
Sbjct: 137 SEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLG 196

Query: 191 KLLTSTGDSGGKPYMA-LRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHNDGLQI-FK 250
            L+     SG     + L+LNSYP CPDP   MGLA HTD+SL TIL+Q +  GL+I   
Sbjct: 197 WLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESP 256

Query: 251 DEVG--WIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDPPA 310
            E G  WI + P+ G+LVV +GD+ HI+SNG+  S +HR ++ +   HR S AYF  PP 
Sbjct: 257 QEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKT-HHRVSAAYFAGPPK 316

Query: 311 DLYISPYCKPLSESPQIPLYKCVNVKEYFTIKAKKEGKGLSAIK 350
           +L I P     S+    P+Y+ +  +EY   KA    K L+  +
Sbjct: 317 NLQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of Lsi08G011560 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 4.0e-44
Identity = 114/330 (34.55%), Postives = 175/330 (53.03%), Query Frame = 0


Query: 9   PSQLVPLDFNSIQTVPDSHLWPNSNELSPAIESDERVSLPKIDLLDDGASEL--IGKACE 68
           P + V +D    + + DS L   S+E+ P I+     S+  +D      SEL  +  AC+
Sbjct: 30  PPRYVRVDQEKTEILNDSSL---SSEI-PVIDMTRLCSVSAMD------SELKKLDFACQ 89

Query: 69  KWGMFQLINHGVLKTLLDEAEEETRCLFALPARQKMKTLPSTCSPTTTGYGMAAISKYHQ 128
            WG FQL+NHG+  + L++ E E +  F LP ++K K      S    G+G   I   +Q
Sbjct: 90  DWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQR--SGEFEGFGQVNIVSENQ 149

Query: 129 KLMWHEGFTILGSPVDDAKK-LWPNDYKRFCDVMEEYQSQMKGLGERLIRLIFKFLDISE 188
           KL W + F +   P+   K  L+      F + +E Y S++K + + L   +   L+I  
Sbjct: 150 KLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILFAKMASVLEIKH 209

Query: 189 EKMMKLLTSTGDSGGKPYMALRLNSYPPCPDPDQVMGLAAHTDTSLFTILHQAHN-DGLQ 248
           E+M  L           + ++++N YPPCP PDQVMGL  H+D +  TIL Q +  +GLQ
Sbjct: 210 EEMEDLFDDV-------WQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGLQ 269

Query: 249 IFKDEVGWIPLSPMSGTLVVNVGDMLHILSNGRLPSILHRVMIQENKEHRFSLAYFYDPP 308
           I KD   W+ + P+   LVVNVG++L I++NGR  SI HR ++   KE R S+A F+ P 
Sbjct: 270 IKKDG-KWVVVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKE-RLSVAMFHSPG 329

Query: 309 ADLYISPYCKPLSESPQIPLYKCVNVKEYF 335
            +  I P  K L +  +  L+K ++ +EYF
Sbjct: 330 KETIIRP-AKSLVDRQKQCLFKSMSTQEYF 337

The following BLAST results are available for this feature:

BLAST of Lsi08G011560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	3.2e-78	44.41	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q9ZT84	3.3e-75	44.51	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q39103	4.7e-74	42.98	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9C971	1.1e-65	39.08	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9SVS8	2.9e-63	39.53	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of Lsi08G011560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q9LM06	1.0e-180	87.71	Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE...	[more]
S0BE47	2.6e-168	80.86	GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1	[more]
A0A1S3BBH9	5.1e-164	80.06	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE...	[more]
A0A6J1KKU0	7.1e-150	74.57	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11149661...	[more]
A0A6J1GIY0	8.7e-148	73.43	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454...	[more]

BLAST of Lsi08G011560 vs. NCBI nr


Match Name	E-value	Identity	Description
AAF80661.1	2.1e-180	87.71	putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris]	[more]
XP_038885582.1	1.5e-170	82.67	gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida]	[more]
NP_001275523.1	5.4e-168	80.86	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetic...	[more]
XP_008445138.1	1.1e-163	80.06	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]
XP_023002882.1	1.5e-149	74.57	gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima]	[more]

BLAST of Lsi08G011560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80340.1	2.3e-76	44.51	gibberellin 3-oxidase 2	[more]
AT1G15550.1	3.3e-75	42.98	gibberellin 3-oxidase 1	[more]
AT1G80330.1	7.5e-67	39.08	gibberellin 3-oxidase 4	[more]
AT4G21690.1	2.0e-64	39.53	gibberellin 3-oxidase 3	[more]
AT1G78550.1	4.0e-44	34.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 262..288 score: 30.3 coord: 61..78 score: 46.46 coord: 226..247 score: 38.84
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 11..341
None	No IPR available	PANTHER	PTHR47991:SF77	GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKE	coord: 11..341
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 13..341
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 11..335 e-value: 1.2E-78 score: 266.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 57..149 e-value: 4.1E-17 score: 63.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 205..303 e-value: 2.2E-27 score: 95.5
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 201..303 score: 12.816041

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G011560.1	Lsi08G011560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding