Lsi08G011250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G011250
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAminoacyl-tRNA hydrolase
Locationchr08: 19891091 .. 19893846 (+)
RNA-Seq ExpressionLsi08G011250
SyntenyLsi08G011250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTATGATTTATTTTGATTTTGGGACATTTGATTCAGTTGAGAAAGGATTTTAGATGGGATTTGATTGAAAATAAATGGGATTTTCTTCACCAAAATCTCAAAATCCCCAAATCCCTCCCCATTCTTGTCTCATCGCATCGATAAAATCAGTTTTTTTTTTTTTTTTTTTTGCTAAGGAAAGTGGGAAAAACCAGAATATAATGTTCAACCAGAATCAAGTTGTAGATATGATGAACATGAAAACGTGGGTTTTTCTAAGTTGATGTGCATTGTTTGTTTATGGTTCTAAGATTTAATTCTTGGAAAATTCAACCCAAGTTGTAGACATGATGATGATGGTTGAAATTAGCTTAGAACTAATCTTTTTACATTATATTATTTTTAATAAATTTACCAATGAATAAAAATTTAATTGAAATTTAATTGTTTTAATAATTTGACCTAATAAACATAAATTGACTAACAAAAACTCTTTAAAGCACACACCAACTAAACAAAAACTATAAAGATGAAGTTTTTTTTGATAAATGTGTAAATTCGCTTTATTCACATCATAAAATTGGCTTCATAAATATTTGTAGAATTCCATCATATTAAGTTTTCTATAACATAATCCTTTATATTATATAAATTACATAATTATTTATTTCCAAGGAAAATCTAATAACATGAGGTTCATGCACAAACAAACACTGCGATCTCTTATTCCCAGTAACACGGTTTCAGCTATCATGACTTGTGTAATTTCAGCTTCTCAATTCGCATTCTCCATTTCCAAATCCACGAGGCTCTGCTCAAATTCAAGGCCTAGAGTTCTCCCATCTTCTTTCCTGAGACCCCGCTCTTCGAATCGAAATTCTAATCCATTTAGCTCTGCCTCCATGAGTGAATCCGCTGAAAGTAATTCTGTTCCTCCACCAGACGATGGCGGAGCGAAGGCAGAGGAGACAGCCGATGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATTTGATTGATTCGTGGCCGCTCGGTAGCGTCGTAACTCAGGGATGCCATGCTTCAGTCTCTGCCATTTGGTTGAATAAAGAAGATCCTCACACTACTGACTACTGTAGTCCACACAATATCGATTCTATGCACAAGGTAAGTCGAATTCTTAACTCCATTTCTAAATTTTAGTTTTCAGTGAAGTGCCTCGAACTCGAATAGTGTAGTATGCATCTATAGACTTGATCATTTTCTGTTAATACGCTGAAGATTTGCTTTATTGGTGCTCTGTTAAGTTGGTCTGACAGTTGATGGATGAGACATTATGAGCAATTTGGAACTTTTGAGTAGCGGTGTTAAAAAAATTCGATGACCGAAAAATTCGATCAATCCAACTTAATTCTTTTAGATTCCATTAAAATAATCGGAACTAATCTAGACTTATTATTAATTTTAAAATGCATATCTTTTTTTACTTAATATAAAATTTTATATATATATTAGTTTTATTTAATTTTGCATTTTTTTATAACTTTTTCTCCCAACAACTTCTAAAAATCATTTTTTTAGTACTTTGAAAAGAAAATTTTTATAATATAAATTGAAATTGAATCATTAATTCTAATTCAATATAAGAAAATAATTGAATAATTTTTACCTATTAACATTAACAAAAATGTAAATAAATGACCCGAATAATCCAAACTAATCCAACCTAAATGTTTCATGATTGGGTTGGGTTGAGTTCATTTTTTAATAAAGGATATTTAGGTTAAGAAATTTATAGCCCGAACAATTGAGTTGAATCTAAAAAGTTCTTTAACCCAACCCAATCCATGAATACCACTACTTTTGCCGGTTGATTTATTTAGTTATGGAAAGAACATTTTATTATGTCTATCAAGCATTTTAGAAGAACAATTGGTTATTGAAAGATATATCTCTGTTATTCTAAAGTTTGAATCCTACTTGGTTGTATTGTTAGTTCAAACAGAGATCATTACTTTCTTATCTCTACTTCTACTTCTACATTTCACTAACTCCTAAAGTTGTTGATTTAACTTTATGTAGGTTACTCTAGAAGTCAAGGGAGAAACTCAGATGGTTAACTTGTCAGAGAAACTTAAAGCAAACTCAATCATACATAAACTATGGATAGAACAACCAGAAAACATTCCAACATGTCTTGCCACAAAACCTTACCCCAAATCTGTTGTATCACCCTTTTTTAAAAAGCTAAAACTCTGTAAGTGATTGAATATTCAAATTACAAAGAACTGATGAATGAGAAGACTCTCCTGAACTCAACTATAAAAGGGTTGCTAATTTTTCATTCCCCTCTTCTAATTCATGAATAATCATACTTTTTTCTGAAGACACCCAGAATTACAAACTATTTCTGCAGAGATGATGCAGCAAACACATAGTAGCTCATAATCATAAAAGAGAAACGAGGGAGAAGGAAAGGCCAATATTTCCAATCAAAGGTACGTAAATTTTACACAAACACTGATCAAAATTACAAAACAAAGATGAAAATAAGTCAACCCCACTTTCTAAACTTCACAGATGATGGAAAAAAACAACAATTCTGTAAGATAACAGTGTTTTTTTTTTTTTTTTCTCTACTTCATAACGAGTTGTCTGGTGCACATAGTGCAATAGAACTTTCGTTTGGTTTTCCAGTAAAGAGGGAGGAAACAGAACCTCCATTGGCTTTCAATGTCGGTGGCTTGGATAATACCTCCGCAGAAGGGGCATGCCCCTGGAGCTGGTTGCCTTGA

mRNA sequence

ATGGGAAAGTGGGAAAAACCAGAATATAATGTTCAACCAGAATCAAGTTGTAGATATGATGAACATGAAAACGTGGCTTCTCAATTCGCATTCTCCATTTCCAAATCCACGAGGCTCTGCTCAAATTCAAGGCCTAGAGTTCTCCCATCTTCTTTCCTGAGACCCCGCTCTTCGAATCGAAATTCTAATCCATTTAGCTCTGCCTCCATGAGTGAATCCGCTGAAAGTAATTCTGTTCCTCCACCAGACGATGGCGGAGCGAAGGCAGAGGAGACAGCCGATGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATTTGATTGATTCGTGGCCGCTCGGTAGCGTCGTAACTCAGGGATGCCATGCTTCAGTCTCTGCCATTTGGTTGAATAAAGAAGATCCTCACACTACTGACTACTGTAGTCCACACAATATCGATTCTATGCACAAGGTTACTCTAGAAGTCAAGGGAGAAACTCAGATGGTTAACTTGTCAGAGAAACTTAAAGCAAACTCAATCATACATAAACTATGGATAGAACAACCAGAAAACATTCCAACATGTCTTGCCACAAAACCTTACCCCAAATCTGTTAGGGAGGAAACAGAACCTCCATTGGCTTTCAATGTCGGTGGCTTGGATAATACCTCCGCAGAAGGGGCATGCCCCTGGAGCTGGTTGCCTTGA

Coding sequence (CDS)

ATGGGAAAGTGGGAAAAACCAGAATATAATGTTCAACCAGAATCAAGTTGTAGATATGATGAACATGAAAACGTGGCTTCTCAATTCGCATTCTCCATTTCCAAATCCACGAGGCTCTGCTCAAATTCAAGGCCTAGAGTTCTCCCATCTTCTTTCCTGAGACCCCGCTCTTCGAATCGAAATTCTAATCCATTTAGCTCTGCCTCCATGAGTGAATCCGCTGAAAGTAATTCTGTTCCTCCACCAGACGATGGCGGAGCGAAGGCAGAGGAGACAGCCGATGTTTTGATTCAGTACGTGGTGCTCCGGAGAGATTTGATTGATTCGTGGCCGCTCGGTAGCGTCGTAACTCAGGGATGCCATGCTTCAGTCTCTGCCATTTGGTTGAATAAAGAAGATCCTCACACTACTGACTACTGTAGTCCACACAATATCGATTCTATGCACAAGGTTACTCTAGAAGTCAAGGGAGAAACTCAGATGGTTAACTTGTCAGAGAAACTTAAAGCAAACTCAATCATACATAAACTATGGATAGAACAACCAGAAAACATTCCAACATGTCTTGCCACAAAACCTTACCCCAAATCTGTTAGGGAGGAAACAGAACCTCCATTGGCTTTCAATGTCGGTGGCTTGGATAATACCTCCGCAGAAGGGGCATGCCCCTGGAGCTGGTTGCCTTGA

Protein sequence

MGKWEKPEYNVQPESSCRYDEHENVASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDGGAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNIDSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSVREETEPPLAFNVGGLDNTSAEGACPWSWLP
Homology
BLAST of Lsi08G011250 vs. ExPASy Swiss-Prot
Match: Q3SZ85 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 2.2e-24
Identity = 53/105 (50.48%), Postives = 73/105 (69.52%), Query Frame = 0

Query: 95  VLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNIDSMHKV 154
           +L+QY+VLR+DL     SWP G+VV Q CHA+ +A+ L+++ PHT  Y     ++ M KV
Sbjct: 24  ILVQYLVLRKDLTQPPFSWPAGAVVAQACHAATAALHLHRDHPHTAAYL--RELERMRKV 83

Query: 155 TLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPK 197
            LE   ET +  L+E L+  +I HKLW+EQPENIPTC+A +PYPK
Sbjct: 84  VLEAPDETTLKLLAETLQQKNIDHKLWMEQPENIPTCIALRPYPK 126

BLAST of Lsi08G011250 vs. ExPASy Swiss-Prot
Match: D3Z4S3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.1e-22
Identity = 50/105 (47.62%), Postives = 70/105 (66.67%), Query Frame = 0

Query: 95  VLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNIDSMHKV 154
           +L+QY+VLR+DL     SWP G++V Q CHA+ +A+ L+++ PHT  Y     +  M KV
Sbjct: 24  ILVQYLVLRKDLSQAPFSWPTGALVAQACHAATAALHLHRDHPHTAAYL--RELGRMRKV 83

Query: 155 TLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPK 197
            LE   ET +  L+E L+  +I H LW+EQPENI TC+A +PYPK
Sbjct: 84  VLEAADETTLKELAETLQQKNIDHTLWLEQPENIATCIALRPYPK 126

BLAST of Lsi08G011250 vs. ExPASy Swiss-Prot
Match: Q6GMV3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 6.1e-22
Identity = 50/105 (47.62%), Postives = 69/105 (65.71%), Query Frame = 0

Query: 95  VLIQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNIDSMHKV 154
           VL+QY+VLR+DL     SWP G++V Q CHA+ +A+  +++ PHT  Y     +  M KV
Sbjct: 24  VLVQYLVLRKDLSQAPFSWPAGALVAQACHAATAALHTHRDHPHTAAYL--QELGRMRKV 83

Query: 155 TLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPK 197
            LE   ET +  L+E L+  +I H LW+EQPENI TC+A +PYPK
Sbjct: 84  VLEAPDETTLKELAETLQQKNIDHMLWLEQPENIATCIALRPYPK 126

BLAST of Lsi08G011250 vs. ExPASy TrEMBL
Match: A0A6J1HBR1 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 9.3e-74
Identity = 147/173 (84.97%), Postives = 155/173 (89.60%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+F FSISKST+LCS SR RV PSS L  RS NRNSN FSS SMSESAESNS PPPDD 
Sbjct: 7   ASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSAPPPDD- 66

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
               E+TADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNK+DPHT +YCSPHNI
Sbjct: 67  --SVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDPHTFEYCSPHNI 126

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           DSMHKVTLEVKGETQ+VNLSEKL+ANSI HKLWIEQPENIPTCLATKPYPKSV
Sbjct: 127 DSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLATKPYPKSV 176

BLAST of Lsi08G011250 vs. ExPASy TrEMBL
Match: A0A6J1K304 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491924 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 7.8e-73
Identity = 147/173 (84.97%), Postives = 154/173 (89.02%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+F FSISKST+LCS SR RV PSS L  RS NRNSN FSSASMSESAESNS PPPDD 
Sbjct: 7   ASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSAPPPDD- 66

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
               E+ ADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK+DP T++YCSPHNI
Sbjct: 67  --IVEKAADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSPHNI 126

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           DSMHKVTLEVKGETQ+VNLSEKLK NSI HKLWIEQPENIPTCLATKPYPKSV
Sbjct: 127 DSMHKVTLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPYPKSV 176

BLAST of Lsi08G011250 vs. ExPASy TrEMBL
Match: A0A0A0LLQ2 (Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G377990 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 2.3e-72
Identity = 145/174 (83.33%), Postives = 154/174 (88.51%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+F  SISKS  LCS  R RV PSS L   SS R ++PF SASMS+S E+NSV  PDD 
Sbjct: 7   ASRFTLSISKSISLCSCPRSRVFPSSSLSLHSSIRKTHPFCSASMSQSVETNSVSTPDDA 66

Query: 86  GAKAEET-ADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHN 145
           GAKAEET ADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK+DPHT+DYC+PHN
Sbjct: 67  GAKAEETPADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSDYCNPHN 126

Query: 146 IDSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           IDSMHKVTLEVKGETQMVNLSEKLKANSI+HKLWIEQPENIPTCLATKPYPKSV
Sbjct: 127 IDSMHKVTLEVKGETQMVNLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSV 180

BLAST of Lsi08G011250 vs. ExPASy TrEMBL
Match: A0A6J1KJQ6 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 4.8e-70
Identity = 144/173 (83.24%), Postives = 154/173 (89.02%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+F FSIS S RLCSN+R   LPSS     S N NS  FSSASMSESAESNSV P DDG
Sbjct: 7   ASRFTFSISNSARLCSNARV-WLPSSICL-LSWNWNSISFSSASMSESAESNSVLPLDDG 66

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
           GAKAEE ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK+DPHT++YCSP NI
Sbjct: 67  GAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPQNI 126

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           DSMHKVTLEVKGETQ+VNLSEKLKANSI+HKLW+EQPENIPTCLA+KPYPKSV
Sbjct: 127 DSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSV 177

BLAST of Lsi08G011250 vs. ExPASy TrEMBL
Match: A0A6J1KTS0 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 4.8e-70
Identity = 144/173 (83.24%), Postives = 154/173 (89.02%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+F FSIS S RLCSN+R   LPSS     S N NS  FSSASMSESAESNSV P DDG
Sbjct: 7   ASRFTFSISNSARLCSNARV-WLPSSICL-LSWNWNSISFSSASMSESAESNSVLPLDDG 66

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
           GAKAEE ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK+DPHT++YCSP NI
Sbjct: 67  GAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPQNI 126

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           DSMHKVTLEVKGETQ+VNLSEKLKANSI+HKLW+EQPENIPTCLA+KPYPKSV
Sbjct: 127 DSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSV 177

BLAST of Lsi08G011250 vs. NCBI nr
Match: XP_038884124.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Benincasa hispida])

HSP 1 Score: 313.9 bits (803), Expect = 1.1e-81
Identity = 157/173 (90.75%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+FAFSISKSTRLCS S PRV PSS L  RS NRNSNPFSSASMS+SAE+NSVPPPDDG
Sbjct: 7   ASRFAFSISKSTRLCSCSSPRVSPSSSLSLRSWNRNSNPFSSASMSQSAETNSVPPPDDG 66

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
           GAKAEE ADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK+DPHT+DYCSPHNI
Sbjct: 67  GAKAEEIADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSDYCSPHNI 126

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           DSMHKVTLEVKGETQ+VNLSEKLKANSI+HKLWIEQPENIPTCLATKPYPKS+
Sbjct: 127 DSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSI 179

BLAST of Lsi08G011250 vs. NCBI nr
Match: KAG6585426.1 (putative peptidyl-tRNA hydrolase PTRHD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 304.7 bits (779), Expect = 6.8e-79
Identity = 164/226 (72.57%), Postives = 176/226 (77.88%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+F FSIS S+RLC N+R   LPSS    RS N NS  FSSASMSES ES+SV P DDG
Sbjct: 7   ASRFTFSISNSSRLCWNARV-WLPSSICL-RSWNWNSISFSSASMSESGESSSVLPLDDG 66

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
           GAKAEE ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK+DPHT++YCSP NI
Sbjct: 67  GAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPQNI 126

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV------- 205
           DSMHKVTLEVKGETQ+VNLSEKLKANSI+HKLW+EQPENIPTCLA+KPYPKSV       
Sbjct: 127 DSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSPFFKK 186

Query: 206 ----------------REETEPPLAFNVGGLDNTSAEGACPWSWLP 229
                           REETEPP  F VGGLD  SA G CP SWLP
Sbjct: 187 LKLSVAIELAFTFPVEREETEPPFGFYVGGLDRASAVGTCPPSWLP 230

BLAST of Lsi08G011250 vs. NCBI nr
Match: XP_023546189.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 290.8 bits (743), Expect = 1.0e-74
Identity = 149/173 (86.13%), Postives = 157/173 (90.75%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS F FSISKST+LCS SR RV PSS L  RS NRNSN FSSASMSESAESNS PPPDDG
Sbjct: 18  ASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSAPPPDDG 77

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
               E+TADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNK+DPHT++YCSPHNI
Sbjct: 78  ---VEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDPHTSEYCSPHNI 137

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           DSMHKVTLEVKGETQ+VNLSEKLKA+SI HKLWIEQPENIPTCLATKPYPKSV
Sbjct: 138 DSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPENIPTCLATKPYPKSV 187

BLAST of Lsi08G011250 vs. NCBI nr
Match: XP_022962062.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita moschata])

HSP 1 Score: 286.6 bits (732), Expect = 1.9e-73
Identity = 147/173 (84.97%), Postives = 155/173 (89.60%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+F FSISKST+LCS SR RV PSS L  RS NRNSN FSS SMSESAESNS PPPDD 
Sbjct: 7   ASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSAPPPDD- 66

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
               E+TADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNK+DPHT +YCSPHNI
Sbjct: 67  --SVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDPHTFEYCSPHNI 126

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           DSMHKVTLEVKGETQ+VNLSEKL+ANSI HKLWIEQPENIPTCLATKPYPKSV
Sbjct: 127 DSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLATKPYPKSV 176

BLAST of Lsi08G011250 vs. NCBI nr
Match: XP_022996792.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita maxima])

HSP 1 Score: 283.5 bits (724), Expect = 1.6e-72
Identity = 147/173 (84.97%), Postives = 154/173 (89.02%), Query Frame = 0

Query: 26  ASQFAFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDG 85
           AS+F FSISKST+LCS SR RV PSS L  RS NRNSN FSSASMSESAESNS PPPDD 
Sbjct: 7   ASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSAPPPDD- 66

Query: 86  GAKAEETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNI 145
               E+ ADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNK+DP T++YCSPHNI
Sbjct: 67  --IVEKAADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPQTSEYCSPHNI 126

Query: 146 DSMHKVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKSV 199
           DSMHKVTLEVKGETQ+VNLSEKLK NSI HKLWIEQPENIPTCLATKPYPKSV
Sbjct: 127 DSMHKVTLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPYPKSV 176

BLAST of Lsi08G011250 vs. TAIR 10
Match: AT5G10700.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 185.7 bits (470), Expect = 4.3e-47
Identity = 101/168 (60.12%), Postives = 118/168 (70.24%), Query Frame = 0

Query: 30  AFSISKSTRLCSNSRPRVLPSSFLRPRSSNRNSNPFSSASMSESAESNSVPPPDDGGAKA 89
           AF     TR  S   PR+    F        +   F++AS S S ++  +    D  + A
Sbjct: 9   AFRFPIVTRRLSAPAPRI---RFYHSTIRFAHFRRFTTASPSMSQQTGEIDAVVD-ASSA 68

Query: 90  EETADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKEDPHTTDYCSPHNIDSMH 149
           E+  DV++QYVVLRRDLIDSWPLGSVVTQGCHASV+AIW  K+DP T  YC P +IDSMH
Sbjct: 69  EKPDDVVVQYVVLRRDLIDSWPLGSVVTQGCHASVAAIWSFKDDPVTLQYCDPQHIDSMH 128

Query: 150 KVTLEVKGETQMVNLSEKLKANSIIHKLWIEQPENIPTCLATKPYPKS 198
           KVTLEVKGETQM+NLSEKLK   I HKLW+EQPENIPTC+ATKPYPKS
Sbjct: 129 KVTLEVKGETQMMNLSEKLKLGGISHKLWMEQPENIPTCIATKPYPKS 172

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3SZ852.2e-2450.48Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=... [more]
D3Z4S32.1e-2247.62Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 ... [more]
Q6GMV36.1e-2247.62Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1HBR19.3e-7484.97Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1[more]
A0A6J1K3047.8e-7384.97Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491924 PE=4 SV=1[more]
A0A0A0LLQ22.3e-7283.33Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G377990 PE=4 SV=1[more]
A0A6J1KJQ64.8e-7083.24Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1[more]
A0A6J1KTS04.8e-7083.24Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038884124.11.1e-8190.75putative peptidyl-tRNA hydrolase PTRHD1 [Benincasa hispida][more]
KAG6585426.16.8e-7972.57putative peptidyl-tRNA hydrolase PTRHD1, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023546189.11.0e-7486.13putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo][more]
XP_022962062.11.9e-7384.97putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita moschata][more]
XP_022996792.11.6e-7284.97putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G10700.14.3e-4760.12Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023476Peptidyl-tRNA hydrolase II domain superfamilyGENE3D3.40.1490.10Bit1coord: 96..203
e-value: 1.7E-14
score: 55.8
IPR023476Peptidyl-tRNA hydrolase II domain superfamilySUPERFAMILY102462Peptidyl-tRNA hydrolase IIcoord: 97..201
IPR002833Peptidyl-tRNA hydrolase, PTH2PFAMPF01981PTH2coord: 98..198
e-value: 1.5E-20
score: 73.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..88
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..78
NoneNo IPR availableCDDcd02429PTH2_likecoord: 96..197
e-value: 1.7227E-59
score: 181.448
IPR042237Putative peptidyl-tRNA hydrolase PTRHD1PANTHERPTHR46194PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATEDcoord: 62..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G011250.1Lsi08G011250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity