Lsi08G010940 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G010940
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionUbiquitin-conjugating enzyme E2
Locationchr08: 19545344 .. 19550820 (-)
RNA-Seq ExpressionLsi08G010940
SyntenyLsi08G010940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACGTCTGTGTTATGTGGTGATGGGTAACAGCATGAAGTATTTGGATTTTCTCCGAAGATCTTATATTTTTCTGGATTCATCTGCATTTTTTTCCCTATATAATTGTAATATTATATGGTTTCTCTCAAGCCAGCCTTTTGTTATAACCCTGTATGATAGTGGATTTCTACCTCATACTTACCTATAATTATAGATGACTAATTGCTTTTGTATCTTTATGGTTATCGGGTGATTTCAATTAGCTTCATGCATCTTTGTTACTAAACAGCTATTGATACTTTTACTTCTTAGGCTTTAAAAACGTTGAAGATATTATTGTTGTAATAACAAAACTATAGTTTGTAAGTTTTAGTTTGTTAGTTGTTGAATAGTTTGTTTGTTATTTTGATTTGTTAAATTTCTTTAAACTTGTACTCACACTTTCCTTATAAATAAAGGTTATCTCCCCTCATTTAGAGGTAGAGATTCATTTGATTCAATTCTAGAAATTGGTATTACACCATTTATAGAAATAGTTAATCTTGTTAAGTGATAATAGTAGGGATGTTGAAGACAAAAATTTATTGGCAACTGACAGGGGCTTCTTTATTTATTTATTTATTTAACTTTTATCTAATTATTATTATTATGTACAATCACACACATTTAGGATCTGAAAATTGAGTCTTACACCTCATACCTCCTCAAGGTTTATATCTTGCCTCTTTAAGAGGAAAAGCCTCAAGATGCCTTTAGCCTATTAAAACTTTGATTTTGTGTAGGCCTCAAGAGCAAGGTTGTTCAAGGAGTACAAAGAGGTTCAAAGAGAAAAGGTAGCAGATCCAGATATTCAACTGGTTTGTGACGATTCCAATATTTTTAAATGGACAGCCCTAATCAAGGTAAGCCTCTTGATGATTCAGATGAAAAATTGATTTCAGAGTTGTTTCTTACCATGATTGATATGCACTTTATTTAGGGACCGTCAGAGACTCCTTTTGAAGGTGGTGTTTTTCAACTTGCCTTCTCCGTACCTGAGCAATACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGGTAGAAGTCACCCGGATCTTTGTTCTAGATTATGCTAACTATGAGCCCTTCTGTCACAATTGTTAATGTTATTGTTTTTTATGAACTATACTTGGCTAGTATCTGCTCTATATACCGAAATGCCCTACGTGAACTGATGAAGTTACAACCACATTACCTCTAGTAAACTTTGAGAGCCCCCAAATCCCGAATGAGGGAACCCTAAGAAAGCCCACCAACTCCAACTACCTACAGACCATGGCCAATCTATGCTAGATCTGACTAACTTTCCACCAGCAAGATTGTGGTAAGGATTATAAAGAGGTGATCATGATTCATGGGGAGGAAAAGCAACAATATGGGAGGGGGTGTCGTTGTCCAAGGGAGCGTAACTAAATACTGTTGAGCCTCCATGCATATGGGAGTAGGGGGAGAATATATAGAAGGTAATACACGGGCAGGGGAGAGGAATATACAAAAAGAAATTTAGTGGTATTTCATGTGTGGGAGGCCTTGGATTTTCAAATCATCTTGTGGATTCTAGGGAGGCACAACAAGAAATTGAGTAGATGATATCCCTTCTGTTGAAAGGTACAACAGGAGTTGCAATGATGAAATAAGCCAATTTGATGAAACAACCGGTATAATCGTTATAAAATAATTGATGTGGTGTTGCAAACTCTAGGGATATGGGAGTGAACTTAAAAGTTGAAGAACCCTATGGGAGGAGGCAGAGTTGCAATTAAGGTGGATATTTGTATGGGGGAGTTTGACAATGACCTCTCCTTCAAACGTAAAATGTATGCTTGTCAAAAGGATGCTAGTGCTATGATTGAATCTGCCTCGTGGGGAGAAGGTTTGTTTTCATGGTTTGCTGGGGTATGTGCGATCTTGTGGATTTTGTGGGGTGAGCGGAATAGTAAAACTTTTAGAGGGGTGGATAAAGACCCTAGTGAGGTTTGGTCTCTTGTTCGCTTTCATGTTTCTTTGGGGGTTTCCGTTTTGAAGACCTTTTGTAACTACTCTATTGGCGTTATTTTGCATAGTTGGAGTCCCTTCCTGTAGAGGGAGGTTGTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTTTTTTTTTTTTTTTTTTTGTGTGTGTGTGTGAGCATGGCTTTTTGTACGGCCGTGTATTCTTTCATTTCATTCTCAATAAAAGTTGTTTCTATAAAATAAATAAATATATATACACACACATACATATAGATGGGATTGGGAGTGGGAAGCTAGATATGCTGATTTTTTATGGAGAGGATCCAAAAGCGTAGATATATTTTGTGGACTGCTTTCATATGAGAGGAATTCTTGTTGCCGAGCAATTCTGTGCATTAGGGATGGACTAGTGTTGGTGAATGGCGATCATTCACGTTTGTTTGAACCCTTTTGTGACTGTAGTCTTATTTTTTTACGACCTTACTTGAACAGGATCTTTTCTATAGGTTGTACAACTGTGTCTTTGAGTTCTAAGGAGACTGTAGTCTTATTTTACGGTGAACCTTATTTTTAATACTTTTCATTTCTGTCATTAAACATATAAACATAACTCTTTTCAGTGGTTGAATGGAACCACACTTTATACTTATCGCTGTGCCTTCGTGCAGACGGGTGAGATCTGCCTAGATATATTGAAGAATGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCTTTGATGGCTCATCCTGAACCTGATAGCCCGCTAAACTGTGATTCTGGTGAGTTTACATGCTTGCTTATTTGAATCTTTGGATGGTGTGATAACTGGTAATGTAGCAAAGTAGTTAAAGAACTCATTTAATAATGATTTTGTTTTTGAAATTTATACATGTTTTCTTCTATATTCCATACCATGGTTTTCACATTTTCTAGAAAAAATACTTGAATTCTTGCCAATTCTAAAAACAAAAATAAAATTTTGAAACTAAGACTGAAGCAAGTTGATCGGGTAATGTTCAGTTATATATTTTGAAAACAAATCAACTGAATATATTTTAGTAAATGAGATATTAGGAGACCTCAATGGGATGGTCTTCTTGTAAATCCTTTGTGTTGGGGAGGGGACGCCTGTCCCTTTGGCCTTTTTTTTCTTTTTTTTTTTTTTTGTTAAGGGAGATATTAGGGGACCTCACTCTTTGCAATAGGTTCATGACCTACCCGACTAATCTTTGGGAAAGTTTGGCCCTTCCTAAGCCAACTTCTAGAGGCACTAGGATTAGATTTTAAATGATTAAGACCCCATTTGTTAACTATCTGGTTTTTTTGCTTATGAAAATTGTACTTCTTTTTTTCTTTCTAGATGAGTTACTCTTTTCATTGTTAATTGGTTTTGAGATGGAACCCCATATTATCGAATAAAACTCATAGATGGAATTAGTACGGGCCTAAATATGTAGACTTGAACTTAAGACCATGGAGAGAATCTCCCAACATTTCAAATACGAGGGTTTGCCCTTGGAGGTAAATCGACTGAAGCATGTTGCTCGGTTTCGTAAACTATTTCCAGATCAAACTGGGTGACATATAAAGCATAGCATTTTCCCGAGGCAGATGCCAGTGACCTTTAATTCTTTTAATTATTGTGTGGGTGTTTACAGGAAACCTCCTGCGTTCGGGCGACGTCCGAGGATATCAATCAATGGCAAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGCTGATTTGACATTGCTTCCTCTTTGCCAAACATTTGAATTTGACGTTGCTGCTGTCACCAAGGGAGATTGCAGAATGTGTTATTGATCACACCAACAAATTAAATATGATTATCCAGACATCTTATGTAAAGTAGTAAAGCACATTCCATCTTCTGTGTGTTCACTTTTATTTGTTGGTTCACTAGATGGATTGCCTCGTGTTTCCCTTTTCTCATCATTTTAATTGTTCTATGTGTTGATAGTTCTGTCTTCAATATAAGAAATCAATACTTATTTAGATTTCTTTTTTAATTTTAATGTATCTTTTTTTTTAATAAATTAAAAAATGATGTTGAAAATAATGTATTGTTGGAGGTGCTAATAAAAATAGGGGTCTTTGTACAGTATTGAAATATATTGAATTTTGAATAAATCGTCTTTTAG

mRNA sequence

ATGTACGGACCGTCAGAGACTCCTTTTGAAGGTGGTGTTTTTCAACTTGCCTTCTCCGTACCTGAGCAATACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGTGGTTGAATGGAACCACACTTTATACTTATCGCTGTGCCTTCGTGCAGACGGGTGAGATCTGCCTAGATATATTGAAGAATGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCTTTGATGGCTCATCCTGAACCTGATAGCCCGCTAAACTGTGATTCTGGAAACCTCCTGCGTTCGGGCGACGTCCGAGGATATCAATCAATGGCAAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGCTGATTTGACATTGCTTCCTCTTTGCCAAACATTTGAATTTGACGTTGCTGCTGTCACCAAGGGAGATTGCAGAATGTGTTATTGATCACACCAACAAATTAAATATGATTATCCAGACATCTTATGTAAAGTAGTAAAGCACATTCCATCTTCTGTGTGTTCACTTTTATTTGTTGGTTCACTAGATGGATTGCCTCGTGTTTCCCTTTTCTCATCATTTTAATTGTTCTATGTGTTGATAGTTCTGTCTTCAATATAAGAAATCAATACTTATTTAGATTTCTTTTTTAATTTTAATGTATCTTTTTTTTTAATAAATTAAAAAATGATGTTGAAAATAATGTATTGTTGGAGGTGCTAATAAAAATAGGGGTCTTTGTACAGTATTGAAATATATTGAATTTTGAATAAATCGTCTTTTAG

Coding sequence (CDS)

ATGTACGGACCGTCAGAGACTCCTTTTGAAGGTGGTGTTTTTCAACTTGCCTTCTCCGTACCTGAGCAATACCCTTTGCAACCTCCTCAAGTGCGGTTCTTAACGAAAATTTTCCATCCAAATGTTCATTTTAAGTGGTTGAATGGAACCACACTTTATACTTATCGCTGTGCCTTCGTGCAGACGGGTGAGATCTGCCTAGATATATTGAAGAATGCGTGGAGCCCAGCATGGACTCTGCAATCTGTTTGTAGGGCTATAATTGCTTTGATGGCTCATCCTGAACCTGATAGCCCGCTAAACTGTGATTCTGGAAACCTCCTGCGTTCGGGCGACGTCCGAGGATATCAATCAATGGCAAGGATGTACACAAGGCTTGCTGCCATGCCCAAAAAGGGCTGA

Protein sequence

MYGPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARMYTRLAAMPKKG
Homology
[Close]
BLAST of Lsi08G010940 vs. ExPASy Swiss-Prot
Match: Q8LGF7 (Protein PEROXIN-4 OS=Arabidopsis thaliana OX=3702 GN=PEX4 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.4e-58
Identity = 110/131 (83.97%), Postives = 113/131 (86.26%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETP+EGGVFQLAFSVPE YPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+M
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. ExPASy Swiss-Prot
Match: Q86IZ3 (Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum OX=44689 GN=pex4 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 8.4e-40
Identity = 75/124 (60.48%), Postives = 94/124 (75.81%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP +TP+E G F+L  SVP  YPLQPP ++F+TKIFHPN+HFK                T
Sbjct: 39  GPPDTPYENGKFELDISVPTNYPLQPPTIKFVTKIFHPNIHFK----------------T 98

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLD+LK +WS  +TLQSVCR+IIAL++ PE DSPLNCD+GNL+R+GDV+G+ S+ARM
Sbjct: 99  GEICLDLLKTSWSAIYTLQSVCRSIIALLSLPEADSPLNCDAGNLIRNGDVKGHDSLARM 146

Query: 123 YTRL 127
           YTRL
Sbjct: 159 YTRL 146

[Close]
BLAST of Lsi08G010940 vs. ExPASy Swiss-Prot
Match: Q9P6I1 (Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ubc16 PE=3 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 4.8e-27
Identity = 62/129 (48.06%), Postives = 78/129 (60.47%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP+ETP+EGG + L   V E YP+ PP V F TKI HPN+   W N              
Sbjct: 43  GPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNI--SWTN-------------- 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GE+C+DILK  WSPAW+LQS C AII+L+++ +  SPLN D+  LLR+GD   Y S+ R 
Sbjct: 103 GEVCMDILKTHWSPAWSLQSACLAIISLLSNYDASSPLNVDAAKLLRTGDKTAYNSLVRC 155

Query: 123 YTRLAAMPK 132
            T L A  K
Sbjct: 163 TTYLYAKGK 155

[Close]
BLAST of Lsi08G010940 vs. ExPASy Swiss-Prot
Match: O60015 (Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta OX=870730 GN=PEX4 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.2e-23
Identity = 56/143 (39.16%), Postives = 75/143 (52.45%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLT----------------------KIFHP 62
           GP ++PFE  +++L   +P  YPL PP+++F+                       K+ HP
Sbjct: 50  GPDQSPFEDALWKLEIDIPTNYPLDPPKIKFVVFGEEKIRQLQRKTSSGARKVCYKMPHP 109

Query: 63  NVHFKWLNGTTLYTYRCAFVQTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPL 122
           NV+FK                TGEICLDIL+  WSPAWTLQS   AI+ L+A+PEP SPL
Sbjct: 110 NVNFK----------------TGEICLDILQQKWSPAWTLQSALVAIVVLLANPEPLSPL 169

Query: 123 NCDSGNLLRSGDVRGYQSMARMY 124
           N D  NLL+  D   Y+ +   Y
Sbjct: 170 NIDMANLLKCDDTTAYKDLVHYY 176

[Close]
BLAST of Lsi08G010940 vs. ExPASy Swiss-Prot
Match: Q9EQX9 (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus OX=10116 GN=Ube2n PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 3.5e-22
Identity = 54/127 (42.52%), Postives = 77/127 (60.63%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP ++PFEGG F+L   +PE+YP+  P+VRF+TKI+HPNV                  + 
Sbjct: 41  GPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVD-----------------KL 100

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK+ WSPA  +++V  +I AL++ P PD PL  D     +S + +  ++ AR 
Sbjct: 101 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKSNEAQAIET-ARA 149

Query: 123 YTRLAAM 130
           +TRL AM
Sbjct: 161 WTRLYAM 149

[Close]
BLAST of Lsi08G010940 vs. ExPASy TrEMBL
Match: A0A6J1KTV6 (protein PEROXIN-4 OS=Cucurbita maxima OX=3661 GN=LOC111496350 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-58
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. ExPASy TrEMBL
Match: A0A6P4BYD2 (protein PEROXIN-4 OS=Arachis duranensis OX=130453 GN=LOC107470764 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-58
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. ExPASy TrEMBL
Match: A0A0B2QDV8 (Protein PEROXIN-4 OS=Glycine soja OX=3848 GN=D0Y65_014097 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-58
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. ExPASy TrEMBL
Match: I1K9A6 (UBC core domain-containing protein OS=Glycine max OX=3847 GN=100305756 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-58
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. ExPASy TrEMBL
Match: A0A6J1GHB1 (protein PEROXIN-4 OS=Cucurbita moschata OX=3662 GN=LOC111453964 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.4e-58
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. NCBI nr
Match: KAG5031095.1 (hypothetical protein JHK85_015077 [Glycine max] >KAG5147827.1 hypothetical protein JHK82_014708 [Glycine max])

HSP 1 Score: 240.4 bits (612), Expect = 9.2e-60
Identity = 119/136 (87.50%), Postives = 122/136 (89.71%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKW-----LNGTTLYTYRC 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK+     LNG   Y    
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKFENDYLLNGGKSYNVYH 102

Query: 63  AFVQTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQ 122
            + +TGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQ
Sbjct: 103 LW-KTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQ 162

Query: 123 SMARMYTRLAAMPKKG 134
           SMARMYTRLAAMPKKG
Sbjct: 163 SMARMYTRLAAMPKKG 177

[Close]
BLAST of Lsi08G010940 vs. NCBI nr
Match: KAG5045322.1 (hypothetical protein JHK86_014728 [Glycine max])

HSP 1 Score: 240.4 bits (612), Expect = 9.2e-60
Identity = 119/136 (87.50%), Postives = 122/136 (89.71%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKW-----LNGTTLYTYRC 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK+     LNG   Y    
Sbjct: 26  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKFENDYLLNGGKSYNVYH 85

Query: 63  AFVQTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQ 122
            + +TGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQ
Sbjct: 86  LW-KTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQ 145

Query: 123 SMARMYTRLAAMPKKG 134
           SMARMYTRLAAMPKKG
Sbjct: 146 SMARMYTRLAAMPKKG 160

[Close]
BLAST of Lsi08G010940 vs. NCBI nr
Match: KAF1871641.1 (hypothetical protein Lal_00020435 [Lupinus albus])

HSP 1 Score: 235.3 bits (599), Expect = 3.0e-58
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 710 GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 769

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 770 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 824

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 830 YTRLAAMPKKG 824

[Close]
BLAST of Lsi08G010940 vs. NCBI nr
Match: KYP65178.1 (putative ubiquitin carrier protein E2 21 [Cajanus cajan])

HSP 1 Score: 235.3 bits (599), Expect = 3.0e-58
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 253 GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 312

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 313 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 367

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 373 YTRLAAMPKKG 367

[Close]
BLAST of Lsi08G010940 vs. NCBI nr
Match: XP_019415934.1 (PREDICTED: protein PEROXIN-4 isoform X1 [Lupinus angustifolius] >XP_019415935.1 PREDICTED: protein PEROXIN-4 isoform X1 [Lupinus angustifolius] >XP_019415936.1 PREDICTED: protein PEROXIN-4 isoform X1 [Lupinus angustifolius] >XP_019415937.1 PREDICTED: protein PEROXIN-4 isoform X2 [Lupinus angustifolius] >KAE9609153.1 putative aminoacyltransferase, E1 ubiquitin-activating enzyme [Lupinus albus] >OIV96562.1 hypothetical protein TanjilG_24215 [Lupinus angustifolius])

HSP 1 Score: 235.3 bits (599), Expect = 3.0e-58
Identity = 115/131 (87.79%), Postives = 115/131 (87.79%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. TAIR 10
Match: AT5G25760.1 (peroxin4 )

HSP 1 Score: 226.9 bits (577), Expect = 9.8e-60
Identity = 110/131 (83.97%), Postives = 113/131 (86.26%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETP+EGGVFQLAFSVPE YPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+M
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. TAIR 10
Match: AT5G25760.2 (peroxin4 )

HSP 1 Score: 226.9 bits (577), Expect = 9.8e-60
Identity = 110/131 (83.97%), Postives = 113/131 (86.26%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GPSETP+EGGVFQLAFSVPE YPLQPPQVRFLTKIFHPNVHFK                T
Sbjct: 43  GPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFK----------------T 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRG+ SMA+M
Sbjct: 103 GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQM 157

Query: 123 YTRLAAMPKKG 134
           YTRLAAMPKKG
Sbjct: 163 YTRLAAMPKKG 157

[Close]
BLAST of Lsi08G010940 vs. TAIR 10
Match: AT1G16890.1 (ubiquitin-conjugating enzyme 36 )

HSP 1 Score: 101.7 bits (252), Expect = 4.7e-22
Identity = 51/126 (40.48%), Postives = 78/126 (61.90%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP+++P+EGGVF+L   +PE+YP+  P+VRFLTKI+HPN+                  + 
Sbjct: 10  GPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID-----------------KL 69

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK+ WSPA  +++V  +I AL++ P PD PL+ +     +S +    ++ A+ 
Sbjct: 70  GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET-AKE 117

Query: 123 YTRLAA 129
           +TRL A
Sbjct: 130 WTRLYA 117

[Close]
BLAST of Lsi08G010940 vs. TAIR 10
Match: AT1G16890.2 (ubiquitin-conjugating enzyme 36 )

HSP 1 Score: 101.7 bits (252), Expect = 4.7e-22
Identity = 51/126 (40.48%), Postives = 78/126 (61.90%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP+++P+EGGVF+L   +PE+YP+  P+VRFLTKI+HPN+                  + 
Sbjct: 43  GPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID-----------------KL 102

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK+ WSPA  +++V  +I AL++ P PD PL+ +     +S +    ++ A+ 
Sbjct: 103 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET-AKE 150

Query: 123 YTRLAA 129
           +TRL A
Sbjct: 163 WTRLYA 150

[Close]
BLAST of Lsi08G010940 vs. TAIR 10
Match: AT1G16890.3 (ubiquitin-conjugating enzyme 36 )

HSP 1 Score: 101.7 bits (252), Expect = 4.7e-22
Identity = 51/126 (40.48%), Postives = 78/126 (61.90%), Query Frame = 0 

Query: 3   GPSETPFEGGVFQLAFSVPEQYPLQPPQVRFLTKIFHPNVHFKWLNGTTLYTYRCAFVQT 62
           GP+++P+EGGVF+L   +PE+YP+  P+VRFLTKI+HPN+                  + 
Sbjct: 52  GPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNID-----------------KL 111

Query: 63  GEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGYQSMARM 122
           G ICLDILK+ WSPA  +++V  +I AL++ P PD PL+ +     +S +    ++ A+ 
Sbjct: 112 GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKSNEAEAVET-AKE 159

Query: 123 YTRLAA 129
           +TRL A
Sbjct: 172 WTRLYA 159

The following BLAST results are available for this feature:
BLAST of Lsi08G010940 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8LGF71.4e-5883.97Protein PEROXIN-4 OS=Arabidopsis thaliana OX=3702 GN=PEX4 PE=1 SV=1[more]
Q86IZ38.4e-4060.48Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum OX=44689 GN=pex... [more]
Q9P6I14.8e-2748.06Ubiquitin-conjugating enzyme E2 16 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
O600153.2e-2339.16Ubiquitin-conjugating enzyme E2-21 kDa OS=Pichia angusta OX=870730 GN=PEX4 PE=1 ... [more]
Q9EQX93.5e-2242.52Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus OX=10116 GN=Ube2n PE=1 SV... [more]
BLAST of Lsi08G010940 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KTV61.4e-5887.79protein PEROXIN-4 OS=Cucurbita maxima OX=3661 GN=LOC111496350 PE=3 SV=1[more]
A0A6P4BYD21.4e-5887.79protein PEROXIN-4 OS=Arachis duranensis OX=130453 GN=LOC107470764 PE=3 SV=1[more]
A0A0B2QDV81.4e-5887.79Protein PEROXIN-4 OS=Glycine soja OX=3848 GN=D0Y65_014097 PE=3 SV=1[more]
I1K9A61.4e-5887.79UBC core domain-containing protein OS=Glycine max OX=3847 GN=100305756 PE=3 SV=1[more]
A0A6J1GHB11.4e-5887.79protein PEROXIN-4 OS=Cucurbita moschata OX=3662 GN=LOC111453964 PE=3 SV=1[more]
BLAST of Lsi08G010940 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG5031095.19.2e-6087.50hypothetical protein JHK85_015077 [Glycine max] >KAG5147827.1 hypothetical prote... [more]
KAG5045322.19.2e-6087.50hypothetical protein JHK86_014728 [Glycine max][more]
KAF1871641.13.0e-5887.79hypothetical protein Lal_00020435 [Lupinus albus][more]
KYP65178.13.0e-5887.79putative ubiquitin carrier protein E2 21 [Cajanus cajan][more]
XP_019415934.13.0e-5887.79PREDICTED: protein PEROXIN-4 isoform X1 [Lupinus angustifolius] >XP_019415935.1 ... [more]
BLAST of Lsi08G010940 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G25760.19.8e-6083.97peroxin4 [more]
AT5G25760.29.8e-6083.97peroxin4 [more]
AT1G16890.14.7e-2240.48ubiquitin-conjugating enzyme 36 [more]
AT1G16890.24.7e-2240.48ubiquitin-conjugating enzyme 36 [more]
AT1G16890.34.7e-2240.48ubiquitin-conjugating enzyme 36 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 1..129
e-value: 5.5E-20
score: 82.4
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 2..133
NoneNo IPR availablePANTHERPTHR24068:SF383BNAA09G04490D PROTEINcoord: 2..133
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 1..128
e-value: 1.0E-39
score: 137.8
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 3..129
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 59..123
e-value: 1.4E-15
score: 57.2
coord: 2..43
e-value: 8.3E-16
score: 57.9
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 1..129
score: 37.355339
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 3..124
e-value: 6.08386E-40
score: 128.859

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G010940.1Lsi08G010940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0043328 protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component GO:0016020 membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0035091 phosphatidylinositol binding
molecular_function GO:0043130 ubiquitin binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity