Lsi08G005200 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G005200
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAcyl-[acyl-carrier-protein] desaturase
Locationchr08: 13160622 .. 13164315 (+)
RNA-Seq ExpressionLsi08G005200
SyntenyLsi08G005200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAACACTAACCTCAAACCACGTTCCAAGTTATTCTCCGGCTCCATTTGGCCGGAATCTCGCTCACTTCCGGCTGAAGTCTCCGACGCCGCTGTACGCCGTCAGATTCCGGTCATTGGTACGGCCAATTTCGGCCATAGCGGCGCCGGCTCTGGTTAAGGATCAGAGGACGCACTCAATGCCGCCGGAAAAGATTGAAGTGTTCAAGTCGTTGGAAGGTTGGGCGAGTGAGAATGTGTTGCCGCTGCTGAAGCCGGTGGATCAGTGCTGGCAGCCGCAGGATTTCTTGCCGGATTCGACGATGCCGTACGAGGAATTTGTGGAGGAAGTTAGGGCTCTGCGAGATCGGACGGCGGAGCTTCCGGATGAGTACTTTGTGGTGCTGGTTGGAGATATGATTACGGAGGACGCATTGCCGACTTATCAGACGATGATTAATACGCTGGATGGGGTTAAGGATGAGACGGGGGCTAGCCGGAGCCCGTGGGCCACGTGGACCCGGACTTGGACCGCGGAGGAGAACCGCCACGGCGACTTGCTCCGGACATACCTCTATTTATCGGGTCGGGTAGATATGATGATGATTGAACGGACTATCCAGTATTTGATCGGCGCCGGCATGGTGAGACTAATGACCGTTTATTTTTAGTTTTAGTTAACAAAAACTATTTGGTTATATTCATTTTCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTCTTTCATTTTGAAGTATCAAAATAATTGTTTTTTTAGTTTAAGGGCCAAAAATAAATAAAAAAATACAACACCAAAATAATAAAAATTAAAAGTCAAAATGAATCAAAGCTAAAATAGGTCTATAGTATATTTAAACCTAAAGACTTTATTGAGATTTATTAAAAACTTTTAAAAATTTATTTACACTGTTAATTTTAAAAAGGATTGAATTTGATAAGGATATATTAAATAAAGTTCAGGACCGATGATCTATTAGACACTAATGACAGTTTTTTTTTTGGGAGAACAAACTTGTAATTAAAGAAAAACGTTTATTACTAAACATTTTCCTTGAAAAAACATTTATAATATATAATTATTATTTTTTATTAAAATAATATTTTTCAATTTTAGTTTCTATATTTCTAAAAGTTGAATTTTAGTCCTTCCACCCTAATAAATCGCTAGGGTGAATTCTTTCATAACTTTTGTTTTTTTTTATTAACATTTTTCCAAATATGTGTTTCCTTCATGAGAGATCAATTTTAAAAATTAATTTCGATGGATTAAATTTAAAATTAAATTACAAAGTACAGTTAGGTAATACAAATTTCAAAGTACAAGACCCAGTTTACATTTAATTACATTTAAGATGCGAAGCTTACCCAATTTAATAGATTTCGTAAATACCAACTAATTAATTAAGACGGGGCTTTTTTTTTAATTTTTTTTTAAAAAAATAATAATATAATAATGGAGGTTGAGATGTAAATGCGTCCGACGTGGACAGGATCCAGGAACGGAGAACAACCCATACTTGGGATTCGTGTACACGTCATTTCAAGAGCGAGCGACGTTCATATCCCACGGCAACACCGCCCGTTTGGCCAAAGACGGCGGCGATCCACTCCTTGCCCGTATCTGCGGCACCATCGCCTCCGACGAGAAGCGCCACGAGAACGCCTATTGTAGAATCGTCCAGAAGCTTCTCGACGTGGACCCTACTGGCGCCATGCTGGCAATTGCTGACATGATGCACAAGAAGATCACCATGCCGGCGCACCTCATGTACGATGGGCAGGACCCCAAGCTATACGACCACTTCTCCGCCGTGGCTCAGCGGCTTGGAATCTACACGGCTAACGATTACGCCGACATTCTGGAGGTGCTGATCGAACGGTGGAGATTGGAAAAGGTGGAGGGATTGACGGGAGAGGGGCGACGGGCTCAGGACTTCGTGTGTGGGTTAGCGCCGAGGATTAGGAAGCTTCAGGAGCGAGCCGATGAGCGGGCTAAGAAGTTGAAGCCGCATGGGGTCAAGTTCAGCTGGATTTTTAATAAGGAAATTCCCATTCTCTCCTAG

mRNA sequence

ATGCAAACACTAACCTCAAACCACGTTCCAAGTTATTCTCCGGCTCCATTTGGCCGGAATCTCGCTCACTTCCGGCTGAAGTCTCCGACGCCGCTGTACGCCGTCAGATTCCGGTCATTGGTACGGCCAATTTCGGCCATAGCGGCGCCGGCTCTGGTTAAGGATCAGAGGACGCACTCAATGCCGCCGGAAAAGATTGAAGTGTTCAAGTCGTTGGAAGGTTGGGCGAGTGAGAATGTGTTGCCGCTGCTGAAGCCGGTGGATCAGTGCTGGCAGCCGCAGGATTTCTTGCCGGATTCGACGATGCCGTACGAGGAATTTGTGGAGGAAGTTAGGGCTCTGCGAGATCGGACGGCGGAGCTTCCGGATGAGTACTTTGTGGTGCTGGTTGGAGATATGATTACGGAGGACGCATTGCCGACTTATCAGACGATGATTAATACGCTGGATGGGGTTAAGGATGAGACGGGGGCTAGCCGGAGCCCGTGGGCCACGTGGACCCGGACTTGGACCGCGGAGGAGAACCGCCACGGCGACTTGCTCCGGACATACCTCTATTTATCGGGTCGGGTAGATATGATGATGATTGAACGGACTATCCAGTATTTGATCGGCGCCGGCATGGTGAGACTAATGACCGATCCAGGAACGGAGAACAACCCATACTTGGGATTCGTGTACACGTCATTTCAAGAGCGAGCGACGTTCATATCCCACGGCAACACCGCCCGTTTGGCCAAAGACGGCGGCGATCCACTCCTTGCCCGTATCTGCGGCACCATCGCCTCCGACGAGAAGCGCCACGAGAACGCCTATTGTAGAATCGTCCAGAAGCTTCTCGACGTGGACCCTACTGGCGCCATGCTGGCAATTGCTGACATGATGCACAAGAAGATCACCATGCCGGCGCACCTCATGTACGATGGGCAGGACCCCAAGCTATACGACCACTTCTCCGCCGTGGCTCAGCGGCTTGGAATCTACACGGCTAACGATTACGCCGACATTCTGGAGGTGCTGATCGAACGGTGGAGATTGGAAAAGGTGGAGGGATTGACGGGAGAGGGGCGACGGGCTCAGGACTTCGTGTGTGGGTTAGCGCCGAGGATTAGGAAGCTTCAGGAGCGAGCCGATGAGCGGGCTAAGAAGTTGAAGCCGCATGGGGTCAAGTTCAGCTGGATTTTTAATAAGGAAATTCCCATTCTCTCCTAG

Coding sequence (CDS)

ATGCAAACACTAACCTCAAACCACGTTCCAAGTTATTCTCCGGCTCCATTTGGCCGGAATCTCGCTCACTTCCGGCTGAAGTCTCCGACGCCGCTGTACGCCGTCAGATTCCGGTCATTGGTACGGCCAATTTCGGCCATAGCGGCGCCGGCTCTGGTTAAGGATCAGAGGACGCACTCAATGCCGCCGGAAAAGATTGAAGTGTTCAAGTCGTTGGAAGGTTGGGCGAGTGAGAATGTGTTGCCGCTGCTGAAGCCGGTGGATCAGTGCTGGCAGCCGCAGGATTTCTTGCCGGATTCGACGATGCCGTACGAGGAATTTGTGGAGGAAGTTAGGGCTCTGCGAGATCGGACGGCGGAGCTTCCGGATGAGTACTTTGTGGTGCTGGTTGGAGATATGATTACGGAGGACGCATTGCCGACTTATCAGACGATGATTAATACGCTGGATGGGGTTAAGGATGAGACGGGGGCTAGCCGGAGCCCGTGGGCCACGTGGACCCGGACTTGGACCGCGGAGGAGAACCGCCACGGCGACTTGCTCCGGACATACCTCTATTTATCGGGTCGGGTAGATATGATGATGATTGAACGGACTATCCAGTATTTGATCGGCGCCGGCATGGTGAGACTAATGACCGATCCAGGAACGGAGAACAACCCATACTTGGGATTCGTGTACACGTCATTTCAAGAGCGAGCGACGTTCATATCCCACGGCAACACCGCCCGTTTGGCCAAAGACGGCGGCGATCCACTCCTTGCCCGTATCTGCGGCACCATCGCCTCCGACGAGAAGCGCCACGAGAACGCCTATTGTAGAATCGTCCAGAAGCTTCTCGACGTGGACCCTACTGGCGCCATGCTGGCAATTGCTGACATGATGCACAAGAAGATCACCATGCCGGCGCACCTCATGTACGATGGGCAGGACCCCAAGCTATACGACCACTTCTCCGCCGTGGCTCAGCGGCTTGGAATCTACACGGCTAACGATTACGCCGACATTCTGGAGGTGCTGATCGAACGGTGGAGATTGGAAAAGGTGGAGGGATTGACGGGAGAGGGGCGACGGGCTCAGGACTTCGTGTGTGGGTTAGCGCCGAGGATTAGGAAGCTTCAGGAGCGAGCCGATGAGCGGGCTAAGAAGTTGAAGCCGCATGGGGTCAAGTTCAGCTGGATTTTTAATAAGGAAATTCCCATTCTCTCCTAG

Protein sequence

MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHSMPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAELPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDLLRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS
Homology
BLAST of Lsi08G005200 vs. ExPASy Swiss-Prot
Match: Q84VY3 (Stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=S-ACP-DES6 PE=2 SV=2)

HSP 1 Score: 585.5 bits (1508), Expect = 4.6e-166
Identity = 283/380 (74.47%), Postives = 327/380 (86.05%), Query Frame = 0

Query: 26  LKSPTPLYAVRFRSLVRPISAIAA---PALVKDQRTHSMPPEKIEVFKSLEGWASENVLP 85
           + SP+   A R R   + ISA+AA   PAL    + H+MPPEK+E+FKSL+GWA + +LP
Sbjct: 18  MPSPSTFLASRPRGPAK-ISAVAAPVRPALKHQNKIHTMPPEKMEIFKSLDGWAKDQILP 77

Query: 86  LLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAELPDEYFVVLVGDMITEDALPTY 145
           LLKPVDQCWQP  FLPD  +P+ EF ++VR LR+RTA LPDEYFVVLVGDMITEDALPTY
Sbjct: 78  LLKPVDQCWQPASFLPDPALPFSEFTDQVRELRERTASLPDEYFVVLVGDMITEDALPTY 137

Query: 146 QTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDLLRTYLYLSGRVDMMMIERTIQY 205
           QTMINTLDGV+DETGAS S WA+WTR WTAEENRHGDLLRTYLYLSGRVDM+M+ERT+Q+
Sbjct: 138 QTMINTLDGVRDETGASESAWASWTRAWTAEENRHGDLLRTYLYLSGRVDMLMVERTVQH 197

Query: 206 LIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGGDPLLARICGTIA 265
           LIG+GM     DPGTENNPYLGFVYTSFQERATF+SHGNTARLAK  GDP+LARICGTIA
Sbjct: 198 LIGSGM-----DPGTENNPYLGFVYTSFQERATFVSHGNTARLAKSAGDPVLARICGTIA 257

Query: 266 SDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQDPKLYDHFSAVA 325
           +DEKRHENAY RIV+KLL++DP GA+ A+ADMM KKITMPAHLM DG+DP L++HFSAVA
Sbjct: 258 ADEKRHENAYVRIVEKLLEIDPNGAVSAVADMMRKKITMPAHLMTDGRDPMLFEHFSAVA 317

Query: 326 QRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAK 385
           QRL +YTA+DYADILE L+ RWRLEK+EGLTGEG+RAQ+FVCGLA RIR+LQERADERAK
Sbjct: 318 QRLEVYTADDYADILEFLVGRWRLEKLEGLTGEGQRAQEFVCGLAQRIRRLQERADERAK 377

Query: 386 KL-KPHGVKFSWIFNKEIPI 402
           KL K H V FSWIF+K+I +
Sbjct: 378 KLKKTHEVCFSWIFDKQISV 391

BLAST of Lsi08G005200 vs. ExPASy Swiss-Prot
Match: B8A7A3 (Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=OsI_04666 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 3.7e-147
Identity = 251/357 (70.31%), Postives = 299/357 (83.75%), Query Frame = 0

Query: 44  ISAIAAPALVKDQRTHSMPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMP 103
           ++A       + + THSMPPEK EVF+SLEGWA  ++LPLLKPV++CWQP DFLPDS+  
Sbjct: 30  LTAAETATATRRRVTHSMPPEKAEVFRSLEGWARSSLLPLLKPVEECWQPTDFLPDSSS- 89

Query: 104 YEEFVEEVRALRDRTAELPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPW 163
            E F  +V  LR R A LPDEYFVVLVGDMITE+ALPTYQTMINTLDGV+DETGAS  PW
Sbjct: 90  -EMFEHQVHELRARAAGLPDEYFVVLVGDMITEEALPTYQTMINTLDGVRDETGASACPW 149

Query: 164 ATWTRTWTAEENRHGDLLRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYL 223
           A WTRTWTAEENRHGD+L  Y+YLSGRVDM M+E+T+QYLIG+GM     DPGTENNPYL
Sbjct: 150 AVWTRTWTAEENRHGDILGKYMYLSGRVDMRMVEKTVQYLIGSGM-----DPGTENNPYL 209

Query: 224 GFVYTSFQERATFISHGNTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVD 283
           GFVYTSFQERAT +SHGNTARLA+  GD +LAR CGTIA+DEKRHE AY RIV++LL +D
Sbjct: 210 GFVYTSFQERATAVSHGNTARLARAHGDDVLARTCGTIAADEKRHETAYGRIVEQLLRLD 269

Query: 284 PTGAMLAIADMMHKKITMPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIER 343
           P GAMLAIADMMHK+ITMPAHLM+DG+D  L+DHF+AVAQRL +YTA DYADI+E L++R
Sbjct: 270 PDGAMLAIADMMHKRITMPAHLMHDGRDMNLFDHFAAVAQRLNVYTARDYADIVEFLVKR 329

Query: 344 WRLEKVE-GLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEI 400
           W+LE +E GL+GEGRRA+DFVCGLA R+R+  ERA++RAKK +   VKFSWI+++E+
Sbjct: 330 WKLETLETGLSGEGRRARDFVCGLAKRMRRAAERAEDRAKKDEQRKVKFSWIYDREV 379

BLAST of Lsi08G005200 vs. ExPASy Swiss-Prot
Match: Q8LJJ9 (Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0880800 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 3.7e-147
Identity = 251/357 (70.31%), Postives = 299/357 (83.75%), Query Frame = 0

Query: 44  ISAIAAPALVKDQRTHSMPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMP 103
           ++A       + + THSMPPEK EVF+SLEGWA  ++LPLLKPV++CWQP DFLPDS+  
Sbjct: 30  LTAAETATATRRRVTHSMPPEKAEVFRSLEGWARSSLLPLLKPVEECWQPTDFLPDSSS- 89

Query: 104 YEEFVEEVRALRDRTAELPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPW 163
            E F  +V  LR R A LPDEYFVVLVGDMITE+ALPTYQTMINTLDGV+DETGAS  PW
Sbjct: 90  -EMFEHQVHELRARAAGLPDEYFVVLVGDMITEEALPTYQTMINTLDGVRDETGASACPW 149

Query: 164 ATWTRTWTAEENRHGDLLRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYL 223
           A WTRTWTAEENRHGD+L  Y+YLSGRVDM M+E+T+QYLIG+GM     DPGTENNPYL
Sbjct: 150 AVWTRTWTAEENRHGDILGKYMYLSGRVDMRMVEKTVQYLIGSGM-----DPGTENNPYL 209

Query: 224 GFVYTSFQERATFISHGNTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVD 283
           GFVYTSFQERAT +SHGNTARLA+  GD +LAR CGTIA+DEKRHE AY RIV++LL +D
Sbjct: 210 GFVYTSFQERATAVSHGNTARLARAHGDDVLARTCGTIAADEKRHETAYGRIVEQLLRLD 269

Query: 284 PTGAMLAIADMMHKKITMPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIER 343
           P GAMLAIADMMHK+ITMPAHLM+DG+D  L+DHF+AVAQRL +YTA DYADI+E L++R
Sbjct: 270 PDGAMLAIADMMHKRITMPAHLMHDGRDMNLFDHFAAVAQRLNVYTARDYADIVEFLVKR 329

Query: 344 WRLEKVE-GLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEI 400
           W+LE +E GL+GEGRRA+DFVCGLA R+R+  ERA++RAKK +   VKFSWI+++E+
Sbjct: 330 WKLETLETGLSGEGRRARDFVCGLAKRMRRAAERAEDRAKKDEQRKVKFSWIYDREV 379

BLAST of Lsi08G005200 vs. ExPASy Swiss-Prot
Match: Q01753 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Simmondsia chinensis OX=3999 PE=2 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.2e-144
Identity = 243/346 (70.23%), Postives = 292/346 (84.39%), Query Frame = 0

Query: 56  QRTHSMPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALR 115
           Q+THSMPP+KIE+FKSLEGWA ENVL  LKPV++CWQPQDFLPD     E F+++V+ LR
Sbjct: 62  QKTHSMPPQKIEIFKSLEGWAEENVLVHLKPVEKCWQPQDFLPDPAS--EGFMDQVKELR 121

Query: 116 DRTAELPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEEN 175
           +RT E+PDEY VVLVGDMITE+ALPTYQTM+NTLDGV+DETGAS + WA WTR WTAEEN
Sbjct: 122 ERTKEIPDEYLVVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTSWAIWTRAWTAEEN 181

Query: 176 RHGDLLRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERAT 235
           RHGDLL  YLYL+GRVDM  IE+TIQYLIG+GM     DP +ENNPYLGF+YTSFQERAT
Sbjct: 182 RHGDLLNKYLYLTGRVDMKQIEKTIQYLIGSGM-----DPRSENNPYLGFIYTSFQERAT 241

Query: 236 FISHGNTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMM 295
           FISHGNTARLAKD GD  LA++CG IA+DEKRHE AY +IV+KL ++DP GA+LA+ADMM
Sbjct: 242 FISHGNTARLAKDHGDFQLAQVCGIIAADEKRHETAYTKIVEKLFEIDPDGAVLALADMM 301

Query: 296 HKKITMPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGE 355
            KK++MPAHLMYDG+D  L++++SAVAQ++G+YTA DYADILE L+ RW++E + GL+GE
Sbjct: 302 RKKVSMPAHLMYDGKDDNLFENYSAVAQQIGVYTAKDYADILEHLVNRWKVENLMGLSGE 361

Query: 356 GRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPI 402
           G +AQDFVCGLAPRIRKL ERA   +K +    V FSWIFNKE+ +
Sbjct: 362 GHKAQDFVCGLAPRIRKLGERAQSLSKPVSL--VPFSWIFNKELKV 398

BLAST of Lsi08G005200 vs. ExPASy Swiss-Prot
Match: P32061 (Acyl-[acyl-carrier-protein] desaturase, chloroplastic OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.9e-144
Identity = 249/380 (65.53%), Postives = 302/380 (79.47%), Query Frame = 0

Query: 26  LKSPTPLYAVRFRSLVRPISAIAAPAL----VKDQRTHSMPPEKIEVFKSLEGWASENVL 85
           L+SP  + A   RS  R +  +  P +    V  Q THSMPP+K+E+FKSLE WA EN+L
Sbjct: 26  LRSPKFVMASTLRSTSREVETLKKPFMPPREVHLQVTHSMPPQKMEIFKSLEDWAEENLL 85

Query: 86  PLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAELPDEYFVVLVGDMITEDALPT 145
             LKPV++CWQPQDFLPDS   +E F E+VR LR+R  ELPDEYFVVLVGDMITE+ALPT
Sbjct: 86  VHLKPVERCWQPQDFLPDSA--FEGFHEQVRELRERAKELPDEYFVVLVGDMITEEALPT 145

Query: 146 YQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDLLRTYLYLSGRVDMMMIERTIQ 205
           YQTM+NTLDGV+DETGAS +PWA WTR WTAEENRHGDLL  YLYLSGRVDM  +E+TIQ
Sbjct: 146 YQTMLNTLDGVRDETGASPTPWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQVEKTIQ 205

Query: 206 YLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGGDPLLARICGTI 265
           YLIG+GM     DP TENNPYLGF+YTSFQERATFISHGNTARLAK+ GD  LA+ICGTI
Sbjct: 206 YLIGSGM-----DPRTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQICGTI 265

Query: 266 ASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQDPKLYDHFSAV 325
            +DEKRHE AY +IV+KL ++DP G ++A  +MM KK++MPAHLMYDG+D  L+ HFSAV
Sbjct: 266 TADEKRHETAYTKIVEKLFEIDPEGTVIAFEEMMRKKVSMPAHLMYDGRDDNLFHHFSAV 325

Query: 326 AQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERA 385
           AQRLG+YTA DYADILE L+ RW++E + GL+GEG++AQD+VC L  RIRKL+ERA  RA
Sbjct: 326 AQRLGVYTAKDYADILEFLVGRWKVESLTGLSGEGQKAQDYVCALPARIRKLEERAQGRA 385

Query: 386 KKLKPHGVKFSWIFNKEIPI 402
           K  +   + FSWIF++++ +
Sbjct: 386 K--EGPTIPFSWIFDRQVKL 396

BLAST of Lsi08G005200 vs. ExPASy TrEMBL
Match: A0A5D3DJB1 (Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G00630 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.5e-204
Identity = 356/403 (88.34%), Postives = 372/403 (92.31%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           MQTLTSNH+      P  RNL  FRL SP PLY +RF   + PISAIAAPALVK++R HS
Sbjct: 1   MQTLTSNHI------PLTRNLPPFRLNSPPPLYRLRFPPSLHPISAIAAPALVKNRRMHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEKI+VFKSLEGWAS+NVLPLLKPV+ CWQPQDFLPDST+PY EF+EEVRALR+RTAE
Sbjct: 61  MPPEKIDVFKSLEGWASQNVLPLLKPVNDCWQPQDFLPDSTLPYGEFLEEVRALRERTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTR WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRNWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDMMMIERT+QYLIGAGM     DPGTENNPYLGFVYTSFQERATFISHG
Sbjct: 181 LRTYLYLSGRVDMMMIERTVQYLIGAGM-----DPGTENNPYLGFVYTSFQERATFISHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NTARLAK  GDP+LARICGTIASDEKRHENAY RIVQKLL+VDPTGAMLAIADMM KKIT
Sbjct: 241 NTARLAKHRGDPVLARICGTIASDEKRHENAYSRIVQKLLEVDPTGAMLAIADMMQKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ 360
           MPAHLMYDGQDP+L+DHFSAVAQRLGIYTANDYADILEVLIERW LEKVEGLTGEGRRAQ
Sbjct: 301 MPAHLMYDGQDPRLFDHFSAVAQRLGIYTANDYADILEVLIERWGLEKVEGLTGEGRRAQ 360

Query: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS
Sbjct: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 392

BLAST of Lsi08G005200 vs. ExPASy TrEMBL
Match: A0A6J1K3L6 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita maxima OX=3661 GN=LOC111489757 PE=3 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.7e-200
Identity = 342/403 (84.86%), Postives = 371/403 (92.06%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           M +LTSN +PS+SP  FGRNLAHFRLK   PLYAV FR  ++PISAI+ P L+KDQRTHS
Sbjct: 1   MNSLTSNLLPSHSPISFGRNLAHFRLK--PPLYAVNFRPSLQPISAISTPDLIKDQRTHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEK+E+FKSLEGWASENVLPLLKPV QCWQPQDFLPDS +PY+EFV++VRALR+RTAE
Sbjct: 61  MPPEKVELFKSLEGWASENVLPLLKPVQQCWQPQDFLPDSRLPYDEFVDQVRALRERTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRS WA WTR+WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSSWAKWTRSWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDM M+ERT+QYLI +GM     DPGTENNPYLGFVYTSFQERATF+SHG
Sbjct: 181 LRTYLYLSGRVDMQMVERTVQYLISSGM-----DPGTENNPYLGFVYTSFQERATFVSHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NT RLAKD GD  LARICGTIASDEKRHENAY RIV+KLLDVDP+GAMLAI DMM KKIT
Sbjct: 241 NTGRLAKDNGDTTLARICGTIASDEKRHENAYSRIVKKLLDVDPSGAMLAIEDMMRKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ 360
           MPAHLMYDG+DP+L+DHFSAVAQRLGIYTANDYADILEVL+ERW LEKVEGLT EGR+AQ
Sbjct: 301 MPAHLMYDGEDPRLFDHFSAVAQRLGIYTANDYADILEVLVERWALEKVEGLTAEGRQAQ 360

Query: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           D+VCGLAPRIRKLQERADERAKKLKP+GVKFSWIFNKEIPI+S
Sbjct: 361 DYVCGLAPRIRKLQERADERAKKLKPYGVKFSWIFNKEIPIIS 396

BLAST of Lsi08G005200 vs. ExPASy TrEMBL
Match: A0A0A0KCQ9 (Acyl-[acyl-carrier-protein] desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G338660 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.6e-200
Identity = 351/404 (86.88%), Postives = 368/404 (91.09%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           MQTLTSNH+      P  RN+ HFRL SP PLY ++      PISAIAAP LVK +R HS
Sbjct: 1   MQTLTSNHL------PLTRNIPHFRLNSPPPLYRLK-----SPISAIAAPPLVKHRRMHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEKI+VFKSLEGWA++NVLPLLKPVD CWQPQDFLPDST+PY EFVEEVRALRDRTAE
Sbjct: 61  MPPEKIDVFKSLEGWANDNVLPLLKPVDDCWQPQDFLPDSTLPYGEFVEEVRALRDRTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGAS+SPWATWTR WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASQSPWATWTRNWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDM MIERT+QYLIGAGM     DPGTENNPYLGFVYTSFQERATFISHG
Sbjct: 181 LRTYLYLSGRVDMTMIERTVQYLIGAGM-----DPGTENNPYLGFVYTSFQERATFISHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NTARLAK  GDP+LARICGTIASDEKRHENAY RIVQKLL+VDPTGAMLAIADMM KKIT
Sbjct: 241 NTARLAKHNGDPVLARICGTIASDEKRHENAYSRIVQKLLEVDPTGAMLAIADMMQKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVE-GLTGEGRRA 360
           MPAHLMYDGQDP+L+DHFSAVAQRLGIYTANDYADILEVLI+RW LEKVE GLTGEGRRA
Sbjct: 301 MPAHLMYDGQDPRLFDHFSAVAQRLGIYTANDYADILEVLIQRWGLEKVEDGLTGEGRRA 360

Query: 361 QDFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           QD+VCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS
Sbjct: 361 QDYVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 388

BLAST of Lsi08G005200 vs. ExPASy TrEMBL
Match: A0A6J1FH53 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC111445427 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 1.1e-199
Identity = 340/403 (84.37%), Postives = 371/403 (92.06%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           M TLTSN +P++SP  FGRNLAHFRLK   PLYAV FR  ++PISAI+ P L+K Q+THS
Sbjct: 1   MNTLTSNLLPTHSPISFGRNLAHFRLK--PPLYAVNFRPSLQPISAISTPDLIKGQKTHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEK+E+FKSLEGWASENVLPLLKPV QCWQPQDFLPDST+PY+EFV++VRALR+RTAE
Sbjct: 61  MPPEKVELFKSLEGWASENVLPLLKPVQQCWQPQDFLPDSTLPYDEFVDQVRALRERTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGAS S WA WTR+WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASSSSWAKWTRSWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDM M+ERT+QYLI +GM     DPGTENNPYLGFVYTSFQERATF+SHG
Sbjct: 181 LRTYLYLSGRVDMQMVERTVQYLISSGM-----DPGTENNPYLGFVYTSFQERATFVSHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NT RLAKD GD +LARICGTIASDEKRHENAY RIV+KLLDVDP+GAMLAI DMM KKIT
Sbjct: 241 NTGRLAKDNGDTVLARICGTIASDEKRHENAYSRIVKKLLDVDPSGAMLAIEDMMRKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ 360
           MPAHLMYDG+DP+L+DHFSAVAQRLGIYTANDYADILEVL+ERW LEKVEGLT EGR+AQ
Sbjct: 301 MPAHLMYDGEDPRLFDHFSAVAQRLGIYTANDYADILEVLVERWALEKVEGLTAEGRQAQ 360

Query: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           D+VCGLAPRIRKLQERADERAKKLKP+GVKFSWIFNKEIPI+S
Sbjct: 361 DYVCGLAPRIRKLQERADERAKKLKPYGVKFSWIFNKEIPIIS 396

BLAST of Lsi08G005200 vs. ExPASy TrEMBL
Match: A0A1S3B3X2 (Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo OX=3656 GN=LOC103485738 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 5.3e-197
Identity = 340/375 (90.67%), Postives = 355/375 (94.67%), Query Frame = 0

Query: 29  PTPLYAVRFRSLVRPISAIAAPALVKDQRTHSMPPEKIEVFKSLEGWASENVLPLLKPVD 88
           P PLY +RF   + PISAIAAPALVK++R HSMPPEKI+VFKSLEGWAS+NVLPLLKPV+
Sbjct: 47  PPPLYRLRFPPSLHPISAIAAPALVKNRRMHSMPPEKIDVFKSLEGWASQNVLPLLKPVN 106

Query: 89  QCWQPQDFLPDSTMPYEEFVEEVRALRDRTAELPDEYFVVLVGDMITEDALPTYQTMINT 148
            CWQPQDFLPDST+PY EF+EEVRALR+RTAELPDEYFVVLVGDMITEDALPTYQTMINT
Sbjct: 107 DCWQPQDFLPDSTLPYGEFLEEVRALRERTAELPDEYFVVLVGDMITEDALPTYQTMINT 166

Query: 149 LDGVKDETGASRSPWATWTRTWTAEENRHGDLLRTYLYLSGRVDMMMIERTIQYLIGAGM 208
           LDGVKDETGASRSPWATWTR WTAEENRHGDLLRTYLYLSGRVDMMMIERT+QYLIGAGM
Sbjct: 167 LDGVKDETGASRSPWATWTRNWTAEENRHGDLLRTYLYLSGRVDMMMIERTVQYLIGAGM 226

Query: 209 VRLMTDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGGDPLLARICGTIASDEKRH 268
                DPGTENNPYLGFVYTSFQERATFISHGNTARLAK  GDP+LARICGTIASDEKRH
Sbjct: 227 -----DPGTENNPYLGFVYTSFQERATFISHGNTARLAKHRGDPVLARICGTIASDEKRH 286

Query: 269 ENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQDPKLYDHFSAVAQRLGIY 328
           ENAY RIVQKLL+VDPTGAMLAIADMM KKITMPAHLMYDGQDP+L+DHFSAVAQRLGIY
Sbjct: 287 ENAYSRIVQKLLEVDPTGAMLAIADMMQKKITMPAHLMYDGQDPRLFDHFSAVAQRLGIY 346

Query: 329 TANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHG 388
           TANDYADILEVLIERW LEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHG
Sbjct: 347 TANDYADILEVLIERWGLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHG 406

Query: 389 VKFSWIFNKEIPILS 404
           VKFSWIFNKEIPILS
Sbjct: 407 VKFSWIFNKEIPILS 416

BLAST of Lsi08G005200 vs. NCBI nr
Match: XP_038884322.1 (stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Benincasa hispida])

HSP 1 Score: 766.1 bits (1977), Expect = 1.5e-217
Identity = 371/403 (92.06%), Postives = 388/403 (96.28%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           MQTLTSN++ ++SP PFGRNLAH RLKSP+P+YAVR R  +RPISAIAAP+LVKD++THS
Sbjct: 1   MQTLTSNNITTHSPVPFGRNLAHLRLKSPSPIYAVRVRPSIRPISAIAAPSLVKDRKTHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEKI+VFKSLEGWASENVLPLLKPVD+CWQPQDFLPDST+PY EFVEEVRALRDRTAE
Sbjct: 61  MPPEKIDVFKSLEGWASENVLPLLKPVDECWQPQDFLPDSTLPYGEFVEEVRALRDRTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWA WTR WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWAKWTRNWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDMMMIERT+QYLIGAGM     DPGTENNPYLGFVYTSFQERATFISHG
Sbjct: 181 LRTYLYLSGRVDMMMIERTVQYLIGAGM-----DPGTENNPYLGFVYTSFQERATFISHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NTARLAKDGGDP+LARICGTIASDEKRHENAY RIVQKLLDVDPTGAMLAIADMMHKKIT
Sbjct: 241 NTARLAKDGGDPVLARICGTIASDEKRHENAYSRIVQKLLDVDPTGAMLAIADMMHKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ 360
           MPAHLMYDGQDP+L+DHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ
Sbjct: 301 MPAHLMYDGQDPRLFDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ 360

Query: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS
Sbjct: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 398

BLAST of Lsi08G005200 vs. NCBI nr
Match: KAA0058877.1 (stearoyl-(acyl-carrier-protein) 9-desaturase 6 [Cucumis melo var. makuwa] >TYK23746.1 stearoyl-(acyl-carrier-protein) 9-desaturase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 721.8 bits (1862), Expect = 3.2e-204
Identity = 356/403 (88.34%), Postives = 372/403 (92.31%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           MQTLTSNH+      P  RNL  FRL SP PLY +RF   + PISAIAAPALVK++R HS
Sbjct: 1   MQTLTSNHI------PLTRNLPPFRLNSPPPLYRLRFPPSLHPISAIAAPALVKNRRMHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEKI+VFKSLEGWAS+NVLPLLKPV+ CWQPQDFLPDST+PY EF+EEVRALR+RTAE
Sbjct: 61  MPPEKIDVFKSLEGWASQNVLPLLKPVNDCWQPQDFLPDSTLPYGEFLEEVRALRERTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTR WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRNWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDMMMIERT+QYLIGAGM     DPGTENNPYLGFVYTSFQERATFISHG
Sbjct: 181 LRTYLYLSGRVDMMMIERTVQYLIGAGM-----DPGTENNPYLGFVYTSFQERATFISHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NTARLAK  GDP+LARICGTIASDEKRHENAY RIVQKLL+VDPTGAMLAIADMM KKIT
Sbjct: 241 NTARLAKHRGDPVLARICGTIASDEKRHENAYSRIVQKLLEVDPTGAMLAIADMMQKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ 360
           MPAHLMYDGQDP+L+DHFSAVAQRLGIYTANDYADILEVLIERW LEKVEGLTGEGRRAQ
Sbjct: 301 MPAHLMYDGQDPRLFDHFSAVAQRLGIYTANDYADILEVLIERWGLEKVEGLTGEGRRAQ 360

Query: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS
Sbjct: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 392

BLAST of Lsi08G005200 vs. NCBI nr
Match: XP_023550695.1 (stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708.8 bits (1828), Expect = 2.8e-200
Identity = 342/403 (84.86%), Postives = 372/403 (92.31%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           M TLTSN +P++SP  FGRNLAHFRLKS  PLYAV FR  ++PISAIA P L+K Q+THS
Sbjct: 1   MHTLTSNLLPTHSPISFGRNLAHFRLKS--PLYAVNFRPSLQPISAIATPDLIKGQKTHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEK+E+FKSLEGWASENVLPLLKPV QCWQPQDFLPDST+PY+EFV++VRALR+RTAE
Sbjct: 61  MPPEKVELFKSLEGWASENVLPLLKPVQQCWQPQDFLPDSTLPYDEFVDQVRALRERTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGAS S WA WTR+WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASSSSWAKWTRSWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDM M+ERT+QYLI +GM     DPGTENNPYLGFVYTSFQERATF+SHG
Sbjct: 181 LRTYLYLSGRVDMQMVERTVQYLISSGM-----DPGTENNPYLGFVYTSFQERATFVSHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NT RLAKD GD +LARICGTIASDEKRHENAY RIV+KLLDVDP+GAMLAI DMM KKIT
Sbjct: 241 NTGRLAKDNGDTVLARICGTIASDEKRHENAYSRIVKKLLDVDPSGAMLAIEDMMRKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ 360
           MPAHLMYDG+DP+L+DHFSAVAQRLGIYTANDYADILEVL+ERW LEKVEGLT EGR+AQ
Sbjct: 301 MPAHLMYDGEDPRLFDHFSAVAQRLGIYTANDYADILEVLVERWALEKVEGLTAEGRQAQ 360

Query: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           D+VCGLAPRIRKLQERADERAKKLKP+GVKFSWIFNKEIPI+S
Sbjct: 361 DYVCGLAPRIRKLQERADERAKKLKPYGVKFSWIFNKEIPIIS 396

BLAST of Lsi08G005200 vs. NCBI nr
Match: XP_022993893.1 (stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 708.4 bits (1827), Expect = 3.6e-200
Identity = 342/403 (84.86%), Postives = 371/403 (92.06%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           M +LTSN +PS+SP  FGRNLAHFRLK   PLYAV FR  ++PISAI+ P L+KDQRTHS
Sbjct: 1   MNSLTSNLLPSHSPISFGRNLAHFRLK--PPLYAVNFRPSLQPISAISTPDLIKDQRTHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEK+E+FKSLEGWASENVLPLLKPV QCWQPQDFLPDS +PY+EFV++VRALR+RTAE
Sbjct: 61  MPPEKVELFKSLEGWASENVLPLLKPVQQCWQPQDFLPDSRLPYDEFVDQVRALRERTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRS WA WTR+WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSSWAKWTRSWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDM M+ERT+QYLI +GM     DPGTENNPYLGFVYTSFQERATF+SHG
Sbjct: 181 LRTYLYLSGRVDMQMVERTVQYLISSGM-----DPGTENNPYLGFVYTSFQERATFVSHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NT RLAKD GD  LARICGTIASDEKRHENAY RIV+KLLDVDP+GAMLAI DMM KKIT
Sbjct: 241 NTGRLAKDNGDTTLARICGTIASDEKRHENAYSRIVKKLLDVDPSGAMLAIEDMMRKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQ 360
           MPAHLMYDG+DP+L+DHFSAVAQRLGIYTANDYADILEVL+ERW LEKVEGLT EGR+AQ
Sbjct: 301 MPAHLMYDGEDPRLFDHFSAVAQRLGIYTANDYADILEVLVERWALEKVEGLTAEGRQAQ 360

Query: 361 DFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           D+VCGLAPRIRKLQERADERAKKLKP+GVKFSWIFNKEIPI+S
Sbjct: 361 DYVCGLAPRIRKLQERADERAKKLKPYGVKFSWIFNKEIPIIS 396

BLAST of Lsi08G005200 vs. NCBI nr
Match: XP_004148817.1 (stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Cucumis sativus] >KGN47485.1 hypothetical protein Csa_019010 [Cucumis sativus])

HSP 1 Score: 706.4 bits (1822), Expect = 1.4e-199
Identity = 351/404 (86.88%), Postives = 368/404 (91.09%), Query Frame = 0

Query: 1   MQTLTSNHVPSYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHS 60
           MQTLTSNH+      P  RN+ HFRL SP PLY ++      PISAIAAP LVK +R HS
Sbjct: 1   MQTLTSNHL------PLTRNIPHFRLNSPPPLYRLK-----SPISAIAAPPLVKHRRMHS 60

Query: 61  MPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAE 120
           MPPEKI+VFKSLEGWA++NVLPLLKPVD CWQPQDFLPDST+PY EFVEEVRALRDRTAE
Sbjct: 61  MPPEKIDVFKSLEGWANDNVLPLLKPVDDCWQPQDFLPDSTLPYGEFVEEVRALRDRTAE 120

Query: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDL 180
           LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGAS+SPWATWTR WTAEENRHGDL
Sbjct: 121 LPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASQSPWATWTRNWTAEENRHGDL 180

Query: 181 LRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHG 240
           LRTYLYLSGRVDM MIERT+QYLIGAGM     DPGTENNPYLGFVYTSFQERATFISHG
Sbjct: 181 LRTYLYLSGRVDMTMIERTVQYLIGAGM-----DPGTENNPYLGFVYTSFQERATFISHG 240

Query: 241 NTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKIT 300
           NTARLAK  GDP+LARICGTIASDEKRHENAY RIVQKLL+VDPTGAMLAIADMM KKIT
Sbjct: 241 NTARLAKHNGDPVLARICGTIASDEKRHENAYSRIVQKLLEVDPTGAMLAIADMMQKKIT 300

Query: 301 MPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVE-GLTGEGRRA 360
           MPAHLMYDGQDP+L+DHFSAVAQRLGIYTANDYADILEVLI+RW LEKVE GLTGEGRRA
Sbjct: 301 MPAHLMYDGQDPRLFDHFSAVAQRLGIYTANDYADILEVLIQRWGLEKVEDGLTGEGRRA 360

Query: 361 QDFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 404
           QD+VCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS
Sbjct: 361 QDYVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEIPILS 388

BLAST of Lsi08G005200 vs. TAIR 10
Match: AT1G43800.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 585.5 bits (1508), Expect = 3.3e-167
Identity = 283/380 (74.47%), Postives = 327/380 (86.05%), Query Frame = 0

Query: 26  LKSPTPLYAVRFRSLVRPISAIAA---PALVKDQRTHSMPPEKIEVFKSLEGWASENVLP 85
           + SP+   A R R   + ISA+AA   PAL    + H+MPPEK+E+FKSL+GWA + +LP
Sbjct: 18  MPSPSTFLASRPRGPAK-ISAVAAPVRPALKHQNKIHTMPPEKMEIFKSLDGWAKDQILP 77

Query: 86  LLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAELPDEYFVVLVGDMITEDALPTY 145
           LLKPVDQCWQP  FLPD  +P+ EF ++VR LR+RTA LPDEYFVVLVGDMITEDALPTY
Sbjct: 78  LLKPVDQCWQPASFLPDPALPFSEFTDQVRELRERTASLPDEYFVVLVGDMITEDALPTY 137

Query: 146 QTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDLLRTYLYLSGRVDMMMIERTIQY 205
           QTMINTLDGV+DETGAS S WA+WTR WTAEENRHGDLLRTYLYLSGRVDM+M+ERT+Q+
Sbjct: 138 QTMINTLDGVRDETGASESAWASWTRAWTAEENRHGDLLRTYLYLSGRVDMLMVERTVQH 197

Query: 206 LIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGGDPLLARICGTIA 265
           LIG+GM     DPGTENNPYLGFVYTSFQERATF+SHGNTARLAK  GDP+LARICGTIA
Sbjct: 198 LIGSGM-----DPGTENNPYLGFVYTSFQERATFVSHGNTARLAKSAGDPVLARICGTIA 257

Query: 266 SDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQDPKLYDHFSAVA 325
           +DEKRHENAY RIV+KLL++DP GA+ A+ADMM KKITMPAHLM DG+DP L++HFSAVA
Sbjct: 258 ADEKRHENAYVRIVEKLLEIDPNGAVSAVADMMRKKITMPAHLMTDGRDPMLFEHFSAVA 317

Query: 326 QRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKLQERADERAK 385
           QRL +YTA+DYADILE L+ RWRLEK+EGLTGEG+RAQ+FVCGLA RIR+LQERADERAK
Sbjct: 318 QRLEVYTADDYADILEFLVGRWRLEKLEGLTGEGQRAQEFVCGLAQRIRRLQERADERAK 377

Query: 386 KL-KPHGVKFSWIFNKEIPI 402
           KL K H V FSWIF+K+I +
Sbjct: 378 KLKKTHEVCFSWIFDKQISV 391

BLAST of Lsi08G005200 vs. TAIR 10
Match: AT3G02630.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.1e-141
Identity = 241/350 (68.86%), Postives = 283/350 (80.86%), Query Frame = 0

Query: 50  PALVKDQRTHSMPPEKIEVFKSLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVE 109
           P  V  Q  HSMPP+K+E+FKSLEGWA E +L  LKPV++ WQP DFLP+     E F +
Sbjct: 53  PREVHVQVKHSMPPQKLEIFKSLEGWADETLLTYLKPVEKSWQPTDFLPEPES--EGFYD 112

Query: 110 EVRALRDRTAELPDEYFVVLVGDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRT 169
           +V+ LR+R  ELPD+YFVVLVGDMITE+ALPTYQTM+NTLDGV+DETGAS +PWA WTR 
Sbjct: 113 QVKELRERCKELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTPWAIWTRA 172

Query: 170 WTAEENRHGDLLRTYLYLSGRVDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTS 229
           WTAEENRHGDLL  YLYLSGRVDM  IE+TIQYLIG+GM     DP TENNPYLGF+YTS
Sbjct: 173 WTAEENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGM-----DPKTENNPYLGFIYTS 232

Query: 230 FQERATFISHGNTARLAKDGGDPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAML 289
           FQERATFISHGNTARLAKD GD  LA+ICGTIA+DE+RHE AY +IV+KL ++DP G +L
Sbjct: 233 FQERATFISHGNTARLAKDRGDLKLAQICGTIAADERRHETAYTKIVEKLFEIDPDGTIL 292

Query: 290 AIADMMHKKITMPAHLMYDGQDPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKV 349
            +ADMM KKI+MPAHLMYDGQD  L++HFS VAQRLG+YTA DYADILE L+ERW +E +
Sbjct: 293 GLADMMKKKISMPAHLMYDGQDDNLFEHFSTVAQRLGVYTAKDYADILEFLVERWNVETL 352

Query: 350 EGLTGEGRRAQDFVCGLAPRIRKLQERADERAKKLKPHGVKFSWIFNKEI 400
             L+ EG RAQDFVCGL  RIRK++ERA  RAK+   + + FSWIF + I
Sbjct: 353 TDLSSEGHRAQDFVCGLPARIRKIEERAQGRAKEAAKN-IPFSWIFGRNI 394

BLAST of Lsi08G005200 vs. TAIR 10
Match: AT2G43710.2 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 494.2 bits (1271), Expect = 1.0e-139
Identity = 243/388 (62.63%), Postives = 298/388 (76.80%), Query Frame = 0

Query: 14  PAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHSMPPEKIEVFKSLE 73
           P P  R+     L S +P  +   +S+         P  V  Q  HSMPP+KIE+FKS+E
Sbjct: 23  PTPSFRSPKFLCLASSSPALSSGPKSVESLKKPFTPPREVHVQVLHSMPPQKIEIFKSME 82

Query: 74  GWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAELPDEYFVVLVGDM 133
            WA EN+L  LK V++ WQPQDFLPD     + F ++VR LR+R  ELPD+YFVVLVGDM
Sbjct: 83  NWAEENLLIHLKDVEKSWQPQDFLPDPAS--DGFEDQVRELRERARELPDDYFVVLVGDM 142

Query: 134 ITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDLLRTYLYLSGRVDM 193
           ITE+ALPTYQTM+NTLDGV+DETGAS + WA WTR WTAEENRHGDLL  YLYLSGRVDM
Sbjct: 143 ITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDM 202

Query: 194 MMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGGDPL 253
             IE+TIQYLIG+GM     DP TENNPYLGF+YTSFQERATFISHGNTAR AK+ GD  
Sbjct: 203 RQIEKTIQYLIGSGM-----DPRTENNPYLGFIYTSFQERATFISHGNTARQAKEHGDIK 262

Query: 254 LARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQDPK 313
           LA+ICGTIA+DEKRHE AY +IV+KL ++DP G ++A ADMM KKI+MPAHLMYDG++  
Sbjct: 263 LAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDN 322

Query: 314 LYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKL 373
           L+D+FS+VAQRLG+YTA DYADILE L+ RW+++ + GL+GEG +AQD++CGLAPRI++L
Sbjct: 323 LFDNFSSVAQRLGVYTAKDYADILEFLVGRWKIQDLTGLSGEGNKAQDYLCGLAPRIKRL 382

Query: 374 QERADERAKKLKPHGVKFSWIFNKEIPI 402
            ERA  RAK  K   + FSWI ++E+ +
Sbjct: 383 DERAQARAK--KGPKIPFSWIHDREVQL 401

BLAST of Lsi08G005200 vs. TAIR 10
Match: AT2G43710.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 492.7 bits (1267), Expect = 2.9e-139
Identity = 242/388 (62.37%), Postives = 297/388 (76.55%), Query Frame = 0

Query: 14  PAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHSMPPEKIEVFKSLE 73
           P P  R+     L S +P  +   + +         P  V  Q  HSMPP+KIE+FKS+E
Sbjct: 23  PTPSFRSPKFLCLASSSPALSSGPKEVESLKKPFTPPREVHVQVLHSMPPQKIEIFKSME 82

Query: 74  GWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAELPDEYFVVLVGDM 133
            WA EN+L  LK V++ WQPQDFLPD     + F ++VR LR+R  ELPD+YFVVLVGDM
Sbjct: 83  NWAEENLLIHLKDVEKSWQPQDFLPDPAS--DGFEDQVRELRERARELPDDYFVVLVGDM 142

Query: 134 ITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDLLRTYLYLSGRVDM 193
           ITE+ALPTYQTM+NTLDGV+DETGAS + WA WTR WTAEENRHGDLL  YLYLSGRVDM
Sbjct: 143 ITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDM 202

Query: 194 MMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGGDPL 253
             IE+TIQYLIG+GM     DP TENNPYLGF+YTSFQERATFISHGNTAR AK+ GD  
Sbjct: 203 RQIEKTIQYLIGSGM-----DPRTENNPYLGFIYTSFQERATFISHGNTARQAKEHGDIK 262

Query: 254 LARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQDPK 313
           LA+ICGTIA+DEKRHE AY +IV+KL ++DP G ++A ADMM KKI+MPAHLMYDG++  
Sbjct: 263 LAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDN 322

Query: 314 LYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRIRKL 373
           L+D+FS+VAQRLG+YTA DYADILE L+ RW+++ + GL+GEG +AQD++CGLAPRI++L
Sbjct: 323 LFDNFSSVAQRLGVYTAKDYADILEFLVGRWKIQDLTGLSGEGNKAQDYLCGLAPRIKRL 382

Query: 374 QERADERAKKLKPHGVKFSWIFNKEIPI 402
            ERA  RAK  K   + FSWI ++E+ +
Sbjct: 383 DERAQARAK--KGPKIPFSWIHDREVQL 401

BLAST of Lsi08G005200 vs. TAIR 10
Match: AT5G16240.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 488.8 bits (1257), Expect = 4.2e-138
Identity = 238/389 (61.18%), Postives = 295/389 (75.84%), Query Frame = 0

Query: 11  SYSPAPFGRNLAHFRLKSPTPLYAVRFRSLVRPISAIAAPALVKDQRTHSMPPEKIEVFK 70
           S S + +    +H      + ++ +R  S          P  V  Q  +SMPP+K+E+FK
Sbjct: 12  SSSSSVYHHGSSHSHGSKSSRVFTIRSDSTAVGRKLYIPPREVHLQVKYSMPPQKLEIFK 71

Query: 71  SLEGWASENVLPLLKPVDQCWQPQDFLPDSTMPYEEFVEEVRALRDRTAELPDEYFVVLV 130
           SLEGWA++N+L  LKPV++ WQP DFLP+     E F ++V+ LR+R  EL D+Y +VLV
Sbjct: 72  SLEGWANDNLLAYLKPVEKSWQPTDFLPEPES--EGFYDQVKELRERCKELSDDYLIVLV 131

Query: 131 GDMITEDALPTYQTMINTLDGVKDETGASRSPWATWTRTWTAEENRHGDLLRTYLYLSGR 190
           GDMITE+ALPTYQTMINTLDGV+DETGAS +PWA WTR WTAEENRHGDLL  YLYLSGR
Sbjct: 132 GDMITEEALPTYQTMINTLDGVRDETGASPTPWAVWTRAWTAEENRHGDLLNKYLYLSGR 191

Query: 191 VDMMMIERTIQYLIGAGMVRLMTDPGTENNPYLGFVYTSFQERATFISHGNTARLAKDGG 250
           VDM  IE+TIQYLIG+GM     DP TENNPYLGF+YTSFQERATFISHGNTARLAKD G
Sbjct: 192 VDMRQIEKTIQYLIGSGM-----DPKTENNPYLGFIYTSFQERATFISHGNTARLAKDLG 251

Query: 251 DPLLARICGTIASDEKRHENAYCRIVQKLLDVDPTGAMLAIADMMHKKITMPAHLMYDGQ 310
           D  L +ICGTIA+DE+RHE+AY +IV+KL ++DP   ++  ADMM KKI+MPAHLMYDG+
Sbjct: 252 DLTLGKICGTIAADERRHEHAYTKIVEKLFEIDPDTTVVGFADMMRKKISMPAHLMYDGR 311

Query: 311 DPKLYDHFSAVAQRLGIYTANDYADILEVLIERWRLEKVEGLTGEGRRAQDFVCGLAPRI 370
           D  L+DHFS+VAQRLG+YTA DYADIL+ L+ERW +EK+  L+ EG RAQD++CGL  RI
Sbjct: 312 DDNLFDHFSSVAQRLGVYTAKDYADILQHLVERWNVEKLSDLSSEGNRAQDYLCGLPARI 371

Query: 371 RKLQERADERAKKLKPHGVKFSWIFNKEI 400
           RKL+ERA  R K+   + + FSWIF +E+
Sbjct: 372 RKLEERAQGRTKEAAKN-IPFSWIFGREV 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84VY34.6e-16674.47Stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic OS=Arabidopsis tha... [more]
B8A7A33.7e-14770.31Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic OS=Oryza sativa su... [more]
Q8LJJ93.7e-14770.31Stearoyl-[acyl-carrier-protein] 9-desaturase 1, chloroplastic OS=Oryza sativa su... [more]
Q017532.2e-14470.23Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Simmondsia chinen... [more]
P320612.9e-14465.53Acyl-[acyl-carrier-protein] desaturase, chloroplastic OS=Cucumis sativus OX=3659... [more]
Match NameE-valueIdentityDescription
A0A5D3DJB11.5e-20488.34Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1K3L61.7e-20084.86Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita maxima OX=3661 GN=LOC1114897... [more]
A0A0A0KCQ96.6e-20086.88Acyl-[acyl-carrier-protein] desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G33866... [more]
A0A6J1FH531.1e-19984.37Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A1S3B3X25.3e-19790.67Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo OX=3656 GN=LOC103485738 P... [more]
Match NameE-valueIdentityDescription
XP_038884322.11.5e-21792.06stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Benincasa hispida... [more]
KAA0058877.13.2e-20488.34stearoyl-(acyl-carrier-protein) 9-desaturase 6 [Cucumis melo var. makuwa] >TYK23... [more]
XP_023550695.12.8e-20084.86stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Cucurbita pepo su... [more]
XP_022993893.13.6e-20084.86stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic-like [Cucurbita ma... [more]
XP_004148817.11.4e-19986.88stearoyl-[acyl-carrier-protein] 9-desaturase 6, chloroplastic [Cucumis sativus] ... [more]
Match NameE-valueIdentityDescription
AT1G43800.13.3e-16774.47Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT3G02630.11.1e-14168.86Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT2G43710.21.0e-13962.63Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT2G43710.12.9e-13962.37Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT5G16240.14.2e-13861.18Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005067Fatty acid desaturase, type 2PIRSFPIRSF000346Dlt9_acylACP_descoord: 28..401
e-value: 2.4E-174
score: 577.4
IPR005067Fatty acid desaturase, type 2PFAMPF03405FA_desaturase_2coord: 64..395
e-value: 6.0E-151
score: 501.9
IPR005067Fatty acid desaturase, type 2PANTHERPTHR31155ACYL- ACYL-CARRIER-PROTEIN DESATURASE-RELATEDcoord: 47..400
IPR005067Fatty acid desaturase, type 2CDDcd01050Acyl_ACP_Desatcoord: 64..377
e-value: 4.6492E-168
score: 470.596
IPR012348Ribonucleotide reductase-likeGENE3D1.10.620.20Ribonucleotide Reductase, subunit Acoord: 48..401
e-value: 6.0E-166
score: 553.6
NoneNo IPR availablePANTHERPTHR31155:SF31STEAROYL-[ACYL-CARRIER-PROTEIN] 9-DESATURASE 6, CHLOROPLASTICcoord: 47..400
IPR005803Fatty acid desaturase type 2, conserved sitePROSITEPS00574FATTY_ACID_DESATUR_2coord: 319..338
IPR009078Ferritin-like superfamilySUPERFAMILY47240Ferritin-likecoord: 51..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G005200.1Lsi08G005200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006631 fatty acid metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0045300 acyl-[acyl-carrier-protein] desaturase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity