Lsi08G004550 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G004550
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionmetal transporter Nramp5-like
Locationchr08: 12185700 .. 12195406 (-)
RNA-Seq ExpressionLsi08G004550
SyntenyLsi08G004550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCGACGCTGAAGAGAGAGAGATTTTAAAAAGAAATGGGAAGTCAGCAGATGGAAGCACAAATGAGTACGGCCGCGACGCCGTCCTTTGGTGGTGGCAGCAACCGTATAGCGGCAGTTAACACGACGGAGAGAACGACAACTCCGACCAACGCCGTCAAGCCACAGAATCACCAAGAAGAACAAACCACCACCCATCAGGTCGTTGATGATCTCTAAGAAAAAAGAAAGAAAAATTCAATTAAATTAAAGTGAAATTAAATATTTTCTTTTTCTTTTTTAATTTTATGTTAACGTTGATTTATCTAGAAGCCCGGATGGAAAAAGTTTCTATCACACGTTGGTCCTGGCTTCCTCGTCTCGTTGGCTTACCTTGATCCTGGCAACCGTAAGTTTATCGTTCGTCATCCAAAGAAATCGAACAATACTTCAGTGCATCTTGAACGACACTCAAATATTAAATATTTATAAATAAATCTGCAACTTAGATATGTAACTAAATTTTTTTTTTTTTTTTAATTTAGCTTTTGGGCATTATTAATTGTTCAAAAGTTTCAGTAGAATTCACTTTATTAGAAAGTATGCATATACCTTAGATTGTTTTTTCTTTATATATATATATAATATACATTTAATTTTAGTTGTTTGAACTATTTTCTTGCTATTATTTTAATTTTAAAATATTTTTTTTTCAAAGTTGTAACATGACAGACTATATTATGTCTTATTTTTTTTCTGTTAATTTCCAGTGGAAACGGACTTGCAGGCTGGAGCTAATCACGGATTCGAGGTAAACAAAAATCAACAAATAAATGGTAGAAAAAAAAAATATATATATATATATAAAAAACATCATATAATTATAATTAGAACATTATAATAATGGTTAGATGTAAATTTAAATTTACCTACTTTTTAACAGTTACTAAATATAAATTTAAGAGATTTTTCTCTAATAATTTAATGGTGAAAAAAAATTTGATGAGCTGACTAAATTGTTTTTGTAGAGCGAGGTTTTTAAACTCTTTAACTAAACCACTTGAAAATAAAATAGGGGGCCGACCAAATCGGATGAATGGAGTAGGTTTGATTTTGATAAATTTTAATTTAAGATTGTAATACATATAATTTTTTTTAAAAAAAATAAATATATAAGTGCATTTGCTTTTATAAGAAAAGTGATTTTTATTAATATTGTATTTTTCTTAAATTAAAATATAATGTTGAATTGATGGCAGCTTCTTTGGGTGGTGCTTATCGGATTAATATTTGCTCTCATAATCCAATCCCTCGCTGCAAATCTTGGGGTAAGCACAGGTAAGTAATAAGTTTAAAATCTCCCCACAAAATATAATAAATAATAATAATAATAATAAAACAATATTATCGCTAATATTTCAGGAAAGTAATAATTTAATCCATAAACTTTAGTAGGTAACAATTTAGTATCTATATATTTAATTTTATAAATTTTAATGAGTAATAATTTAATCTTTATATTTTAAAATTATTAATAATTTAGGTCTCTAATACAAAAAGGGTAATTATTATAAATGGAAAAAATATCAAACTATGTACAAACATCAAAAATTTCATTGTCTATCAGCAATAAATCTTTTTATCACTGAGTAATAGAAGTCTATTGCAGTCTATCAAGTGAAATTTTTCTATATTTGTAAATATTTTTGCTCATTTTTCTATATTTGAAAACAACACTATAAAAAATCTCATCCAAATTAAGTGTCAATTTTTATTACGTAACAACTTAGTTTTTATAATTTTATAAATAAATTTGATTTACAAACTATTTCTCAATCTATATGTATAAGAAATTTTATTGAAATATTAATAATATTTTTTACAACAATGACTAAATTGGTACAAATTTGAAAGTAATAGACTAAATTGTTACAAACTAAAATTTAAAGAATAAATTTTTACTTTCACAAAAGTTTAGGACTAAAAATATTTTTTAACCAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAGGAAAACACCTTTCAGAAGTGTGCAAAGCCGAATATCCCATATTTGTGAAGTACTGCCTTTGGTTGCTGGCTGAAGTTGCTGTAATAGCCGCCGATATTCCAGAAGGCAATTATTTTATTATTTTATTATTTTATTATTTTATTACACTCTAATTAATCTCGTTAATTCGTGTTTTATTTTTCTTAATTAATTTGTGTTTGGTTGCAGTGATAGGAACAGCCTTTGCTCTGAATATTCTTTTCCATATTCCTGTTTGGGCTGGCGTTCTTCTCACCGGTTTAAGCACCCTTTTACTCCTTGGACTCCAAAGATATGGGGTAAATTAATTAATTAATTAATATTTAAAATTATCTTTTAGTCCTCAAATCTTTTATGCTTATTTGATTTTCCCCTAAACTTTCTATTATTATTCTACAATTTTTAATTGTCAACTTTATATTAAAATAAAGATGATTTTGTGATAATTAATCGGTGACAAACCCTTACATTAGTATATTGGTTGTATTATTAATGGTATGTTGGTGATACATTTTCTATTGGAATATTATAATTGGTATACTTTTCTTAATAGCACAGAAATGGTATATTCACGGATATATTGTATCATATTAAATAATGATAAGTTAGTTATATATATTTTCCTTAAAAAAACAGAATTGATGATATATTTAATAATTAAATTACAAATTTACTCCCTATAATTTGGAAAAAAAATTAAATTTAAATCCTATATATGGTTTTAAAAGCAAATAAAATTTCATCTTTATGATTTGATAAAATTTTACATATAATTTGATAAAACATTAATAAATAGTCTTGTTTTATCAAACAATTGGAAATAATTCTATTTTTTTTTTCAAACTCTAGGGACTGAATTTATAAGGTTACTTATATCAATTAATGTTATAGAAATAATAAGAGTAATATACAATAAAATAATTGACATATTTGGGCTCATATAAATCAAGAGATTAAAAATTAACCACGGCTCACCAGAAAGAAATTTTTTGTGAAAAATGATTACAATCACACAAAATTCTCTCTCCGATCTCAATTACATATCAAAAGTACTCGCTCAAAGATTTTAAATCACTCATACCAATTTTTCACTCTCAAAAAATGTATACAAAATGAAAGTTTAACTAGAATTATCATATTTGATCTTAAAAAGTATTTCAAATTGAGACGATTTGAAACAAAAGTTATAAGTTCGTAGAGTTCACTCCTTTCAATTATTGGTTCGTGTGAAATAACTATTCTTCTAATATTTTGTTAGAAAAGTAACAACGACATAAATTATTGACTATAAAATTATATATTTTGTAAATTAATTATTTTAGTTATGGGAATAAACTGCAGGCGAGGAAGCTGGAATTGCTGATAGCAATATTGGTGTTCATAATGGCGGCGTGTTTCTTCGGGGAAATGAGTTACGTGAAGCCTCCGGCGACCGGCGTTTTAAAAGGAATGTTTGTTCCAAAGCTCAACGGCCAAGGAGCCACCGCCGACGCCATTGCTCTTCTTGGCGCCCTTGTTATGCCGTACCATTTCAATTCAAATTCATCTATCCATTTCTTCCATCACGACCAATTTAATAAAACCGACATGTCGACACTTTTTTTTAAATAAGGAAATTATTATTATTATTAAGTTAAATTATTAAAAAAAAAAAATCGTTCAATTTTGTCATTTGTTTGAAAAAAAATCCTTATACTTTCAAAAGCTACAATATTATCCTTTAAATTTTATAAATGTTTCAAAAATATCTTTGAAATAGAAACACTTTAGAATTTAAGCAGAAATTCAAACATGAAATGGTATTGTAGGTGTTATAAAATAAAAATTTGAATCACCATATCGTTTTAGGTCATCGTCACACAGTTCTAAGTTTTAATTTTTATTTAAAATTCTAATCAATTTATACTCAAAGTGATAGTTTTGAAACGTTTATAAAAGTTGAGAGGTAATGTTGCAATTTTTTTAAAGTATAGGAGTATTAAAAAAATTGAAGAATATTTGTTATAATTTAAAGAAATTATTACTATTATAATTATTAAATAATATTTGGAATATTGAATTTGTGGAACAGGCATAACCTCTTTCTTCATTCGGCTCTCGTGCTGTCAAGGAAAGTCCCAAATTCCGTCCGTGGTATCAACGTGCGTACGCCCTCTCCGTTTCTTCTAATTTTTATTTTTTTTATTTTTTTTTTATTGAAGAATTGCTTCTAATATTATTACATATATATATCCTTTTTTTCCCTTAAATGTGTCTCTACGAAAATATATTTAAGAGAAAAAAAAATACATTTTTACTCCTTAGTTTTTAGATTTAGATTCATTTAATTTCTAATTTTTAAAATGTTACACTTTTAATCTTTTAAATTTTAGGTTTAATTCATAAATTTCAAAATGTTATAATTTTACTCTTGAGATTTGGGTTTTTTTTTTATAATTAAGTTCATATATTTCAAGATTTTACACTTTTAGTCTCTATTTTTACTAAATACTCACTTCAATTTTCAGTGTTAATGTCTATTAATTAATTTAGAATAATTATGAAGTGAAATTTTAAATTTGATTTTAATAGTGATGAAAAATAGTTGAACCTAATTAATTATAATCATTTTAATTCCTTTAAATTGATTAATAGACATTAACATTAAAGACTAAAAGTGAGTATTTGATGTAAAATCGTTAAAAGTCTCCATCTTCAAACTTAAAAATTCAAATTGAAAAAACATAAGAATCAAATGAAAACTATATCGTTCAAAATGAACATAGCTCAACTGAAATAATACTTGTACTATTAATTTTGAAGTAAAAAGTTTAATTCTCCCACCCACATATTAATAATAATAAATAAATAAACAAACTAGACTCAAAATAACAAAATTATAATTGTTTGTTTTATTTAAATAACAAACTTACCCTCAAACAAATAACTAAAATAATTAGTATAAGACTTTTTTTCTTTTTATCTTTTTGAGTTCAAAATAATTAGTATAGTCTTTTTCTTATTTGTATGTTAGTCGAGTTAGGTTCCTTATTAATTTATTTTTCACTAGAAATAAAGAAAAAAAAAGTCAAATTTTAAAATATATATATATATATATATATAAAAGTTTATAAATGCGTGAAATTGGAATTCTGGGAATGCAGGATGCGTGTAGATACTTCCTAATAGAGAGTGGATTTGCATTGTTCATAGCATTTTTAATAAACGTGGCCGTCGTTTCAGTTTCCGGCACCGTTTGTACGGTCGGAAATGTCTCCGAAACCACCGCCGATCAATGCTCCGACATAACTCTCAATTCCGCCTCCTTCCTCCTCCAGGTCCGCGGCCGCTTATCTCCCATTTTACACTCAAAACTAAAAAACGCACCGTTTTGATTATTATTATTATTTTCTAATAAATAAATTTTATTTTAATTTTCAGAACGTTTTGGGAAAATCAAGCTCCATTATTTACGCCATTGCTTTGTTTGCCTCTGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGTCAGTTCATCATGCAGGTATTTTTATTTCAATTATTTTTTCGTTTATTAAATCCAAATTTTTTAATTGCATTTAATTTTTTTTTAAAAAATAAATAAATAAATTTTGTTTAAATTATTATTATTGATTTTAAGGGATTTTTGGACCTGAAAATGAAAACATGGGCTCGAAACCTTATGACAAGGTCCATTGCAATAACGCCTAGTCTCATCGTATCCATCATCGGTGGACCTCAAGGAGCTGGCCGTCTCATCATTATTGCATCGGTTTGTTTTTTTCTATTATTATTATTATTTTATTTTTCTCCCTCAAACTTGGAATTAATAATTAAAGTCTCATTCAAAACATAAATTTGAAAATAAGATACCGGACATTTCGTAGCAATTTTAAAGGAAAATTCTTATAAATAAAAAAATAGAAATGGTTATAACATAAAAAAAATATCAAACTATTTATAAATATAGGAAATTTTTATTTTCTATCGACGATAGCTTGAGCGATAGACAATGATAGAAGTCTAACACTAAAAAAAAATATTTATCGCGGTCTATCGTTGATAACAGTAAAATTTTTCTATGACTCATTTTTCTATATTTAAAAATAACCGTAAAAAATATCAAACTATTTATAAAAATAGAAAATTTTCACTATCTATTAATGATAAATCACGATAGATTTCTATTACTTGAGAGATAGAAGTCTATCGCGGTCTATCACTCAAGCAATAGAAGTGAAACTTTTTTATATTTGTAAATAGTTTTGCTCAATAACCGATTTTAAATTATTAAAATTATTTTAGTCGTTGTTAAAATTATTATAAAATATGTTTTTTAATAATTCCGAACTAATTTTAATGATATGAAAATTGCATTTAAAAGTCTAAAATTAAAAATTAAATTAATTTTACTTTTTTCATCGAAACAAAATTATATTTCATGTTTTCAAATATGTATTTAGTAGTATAGATTAAATAGTTGGATTCCAATTTAAACAATTATTCAAAATGTATTTGATAATATATTTATAAACTATATAACTAGTTGTAGATTTATTTATGAATATGTTTTGTGTTAAAAATGTATAATTATTATTTTGTAATATCATATAATTTATAAAATATATTTTGATTTAACATGGATGAATTGAGTTTATAACATAAAATACTATTTTTATTAATATTTTTTTTTTACCTTTATTTAAATAATTATGAATTTTTAAAATTTCAAATTCAAAATATGTATACATTCAAAACATACAAATATTTTTTGGAATTTTGTCTAAAATTGAAATCAAATGTTCATTTATGCTACATATTAGAGACAGTCAAAAACACTAAAACAAAATCTAAAACAAAATCAATTATCATAGTTATTATAATTACTAAGACCTCATTTGATAACTATTTGATTTTTAGTTCTTAGATTTTAAAAATTAACCTACAAATACCCATTCCATCTCTAATTTTTTTGCTTAGTAATCTACTTTTTATCTATGTTTTCAAAAACTAAGTCAAAACTTGAAAATTAAAAAATATATATATAGTTTTTAAAAATTTATTTTTGTTTTTTTAATTTGGTTAAGATTTCAATTGTATACTTAACAAAGATACCAACTATTGTATAAAAATGAGAGAAAATAGACTTAATTTTTAAAAACAAAAAACAAAAATAGTTATCAAATGAGACTTAAAATATGTCGTTGGTATTTATAACTAATTAAAATGCATTACTATGTGATGCTAACCTTTAATTAAAAATAATAGTCAACAGCAAATTTAAATTCAAATATTTTATTATTATTTCATTTGATGGTAATGTTTTTATTTTAAAATTTTATTTAATATTAAATCATGCTCATAAAATAGAATTACATCATCTAAAAAAATTAAAAATATTTTTCTTATTAAAAAATTATAGGCTAATTATACAAGTATTTTTACTTTAACTAGATAATTAATAATTAAATTTTAAAAAAAAATAGATGTAATCAAAGAATATATAAGTATGCTTTGTTAATTATGAGAGAAACTATTAATAGTGTAAAAATTTGTCAAAATTTTTATTAATGATGTAAATAAGAGAAATACAAAAGGGTAAAATATGTAGTTAACAAAAAAGGTTGTAGACTTTATTTAATAGATATATGAAAGGTGTGAAGATATATAATAATTAATGAAAAAAAATGGTAGAGTTCTCCGAAAGGAATGAGAATTAGATTAATAAGAGTTTAAAAGATGAATAAGAATGAAATTGTTTTAACTAAGGAATTTTAAATTGGGGTTGGGTCCCACCGTTTCATTACTATATTTTGGTAGGAAAATTTTCAGTACCATGGAAGTCGGAAGTATTTCTTGTATGTCATATCAACTTACTTTTAAATATATGTGGGATACACTAACATTAATTTGGGGTTCACTAGTATTTATGAAAACAAATATATAATGTGAACAAATGGAATATAGTATCGGTAGTCCCATATTACTAAATTTTTTCTCCATTTTGGTAAACACTATAGAGATCGAACCTTTTCAATATTATTCATTAATTTTCATTTCCTTTAATTAAATGAGAATAAAATCGAACACTCAAATAAATTAAGGCAAATTGTAAAAACCACCCTTGAGATATGGTTATAGTTGCAATTACACCTTCAAACTTCTACAAGTGTTAAAAATTGGACCCTCAAACTTATAATAATGGTAGAAATTGGACTCACAAATGATAAAAATTGAATTTTCAAACTTATATAAATGTTCAATTTGACCCAATTTAACACTTATGTAATTTTGAGGGCTCAATTTTCAAAATGGAAAGTTTAAGGGTATAATTGCAACTATCACCATACTTCAAGGATTGTTTTTGCAATTTGCCCTAATTTTTTTTATATATAAATATTAATTTTCAAGAATTAATTTAATAATGGGTTAATGGCATAATTTGGGACAGATGATTCTTTCCTTCGAGCTTCCATTTGCTCTAATTCCACTCCTCAAATTCAGTAGCAGCTCCACCAAGATGGGCCCCCACAAGAATTCCATATACGTGAGCCAAATCTTCTCTCTTTTCATTCCTTCGGCCCAAATTACCATTATTGGGCCGGCCCATTAACACGTTTAAACTTATTTGGGCTTTTCTTTCAGATAATTGTAATCTCGTGGATATTGGGCCTGGCAATCATCGGCATCAACATCTATTATCTCAGTACGGCCTTTGTGGGCTGGCTAATCCATAACAATTTACACAGAATTGCAAATGTGTTCATTGGGATCGTTGTTTTTCCATTAATGGCCATCTACGTTCTTGCCGTTATTTACCTTATGTTTCGAAAAGACAGGGTTGTCACTTACATTGAGCCCGAAAAGGATGACCCCATGGACCAAGCCCATTTGGAAAGTGGGCTTAATAATTCTTTTGGGCCGCGTGACGTCACTCCGGTGCCTTTCAGAGAGGACTTGGCCCATATCCCACTACCAGAGTAGAAGCCTTGATAGATAGTTTGGGCTTGTATCCAATTCTGGGCTGATGGGCTTTTACTGCCACCTTGGCCCATTAATGTTTTTGTATTTGCTTCTGTAGGCTTTACTGCAGGCTATTATGTAGATATTTTGCTTGTGTGTCGTGTTTTTTCTTTCTTCTGAATTAATAGCACATGTGGGGGGAGAAATGGGAACTTTTGATCTATTGGTTGAGAGGTTTTATCTAAACCAATTGAGTTATGTTCAAGTTGATACCATGTATGTTACATAATTTCAAACACGTATCATT

mRNA sequence

CAGCGACGCTGAAGAGAGAGAGATTTTAAAAAGAAATGGGAAGTCAGCAGATGGAAGCACAAATGAGTACGGCCGCGACGCCGTCCTTTGGTGGTGGCAGCAACCGTATAGCGGCAGTTAACACGACGGAGAGAACGACAACTCCGACCAACGCCGTCAAGCCACAGAATCACCAAGAAGAACAAACCACCACCCATCAGAAGCCCGGATGGAAAAAGTTTCTATCACACGTTGGTCCTGGCTTCCTCGTCTCGTTGGCTTACCTTGATCCTGGCAACCTGGAAACGGACTTGCAGGCTGGAGCTAATCACGGATTCGAGCTTCTTTGGGTGGTGCTTATCGGATTAATATTTGCTCTCATAATCCAATCCCTCGCTGCAAATCTTGGGGTAAGCACAGTGATAGGAACAGCCTTTGCTCTGAATATTCTTTTCCATATTCCTGTTTGGGCTGGCGTTCTTCTCACCGGTTTAAGCACCCTTTTACTCCTTGGACTCCAAAGATATGGGGCGAGGAAGCTGGAATTGCTGATAGCAATATTGGTGTTCATAATGGCGGCGTGTTTCTTCGGGGAAATGAGTTACGTGAAGCCTCCGGCGACCGGCGTTTTAAAAGGAATGTTTGTTCCAAAGCTCAACGGCCAAGGAGCCACCGCCGACGCCATTGCTCTTCTTGGCGCCCTTGTTATGCCGCATAACCTCTTTCTTCATTCGGCTCTCGTGCTGTCAAGGAAAGTCCCAAATTCCGTCCGTGGTATCAACGATGCGTGTAGATACTTCCTAATAGAGAGTGGATTTGCATTGTTCATAGCATTTTTAATAAACGTGGCCGTCGTTTCAGTTTCCGGCACCGTTTGTACGGTCGGAAATGTCTCCGAAACCACCGCCGATCAATGCTCCGACATAACTCTCAATTCCGCCTCCTTCCTCCTCCAGAACGTTTTGGGAAAATCAAGCTCCATTATTTACGCCATTGCTTTGTTTGCCTCTGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGTCAGTTCATCATGCAGGGATTTTTGGACCTGAAAATGAAAACATGGGCTCGAAACCTTATGACAAGGTCCATTGCAATAACGCCTAGTCTCATCGTATCCATCATCGGTGGACCTCAAGGAGCTGGCCGTCTCATCATTATTGCATCGATGATTCTTTCCTTCGAGCTTCCATTTGCTCTAATTCCACTCCTCAAATTCAGTAGCAGCTCCACCAAGATGGGCCCCCACAAGAATTCCATATACATAATTGTAATCTCGTGGATATTGGGCCTGGCAATCATCGGCATCAACATCTATTATCTCAGTACGGCCTTTGTGGGCTGGCTAATCCATAACAATTTACACAGAATTGCAAATGTGTTCATTGGGATCGTTGTTTTTCCATTAATGGCCATCTACGTTCTTGCCGTTATTTACCTTATGTTTCGAAAAGACAGGGTTGTCACTTACATTGAGCCCGAAAAGGATGACCCCATGGACCAAGCCCATTTGGAAAGTGGGCTTAATAATTCTTTTGGGCCGCGTGACGTCACTCCGGTGCCTTTCAGAGAGGACTTGGCCCATATCCCACTACCAGAGTAGAAGCCTTGATAGATAGTTTGGGCTTGTATCCAATTCTGGGCTGATGGGCTTTTACTGCCACCTTGGCCCATTAATGTTTTTGTATTTGCTTCTGTAGGCTTTACTGCAGGCTATTATGTAGATATTTTGCTTGTGTGTCGTGTTTTTTCTTTCTTCTGAATTAATAGCACATGTGGGGGGAGAAATGGGAACTTTTGATCTATTGGTTGAGAGGTTTTATCTAAACCAATTGAGTTATGTTCAAGTTGATACCATGTATGTTACATAATTTCAAACACGTATCATT

Coding sequence (CDS)

ATGGGAAGTCAGCAGATGGAAGCACAAATGAGTACGGCCGCGACGCCGTCCTTTGGTGGTGGCAGCAACCGTATAGCGGCAGTTAACACGACGGAGAGAACGACAACTCCGACCAACGCCGTCAAGCCACAGAATCACCAAGAAGAACAAACCACCACCCATCAGAAGCCCGGATGGAAAAAGTTTCTATCACACGTTGGTCCTGGCTTCCTCGTCTCGTTGGCTTACCTTGATCCTGGCAACCTGGAAACGGACTTGCAGGCTGGAGCTAATCACGGATTCGAGCTTCTTTGGGTGGTGCTTATCGGATTAATATTTGCTCTCATAATCCAATCCCTCGCTGCAAATCTTGGGGTAAGCACAGTGATAGGAACAGCCTTTGCTCTGAATATTCTTTTCCATATTCCTGTTTGGGCTGGCGTTCTTCTCACCGGTTTAAGCACCCTTTTACTCCTTGGACTCCAAAGATATGGGGCGAGGAAGCTGGAATTGCTGATAGCAATATTGGTGTTCATAATGGCGGCGTGTTTCTTCGGGGAAATGAGTTACGTGAAGCCTCCGGCGACCGGCGTTTTAAAAGGAATGTTTGTTCCAAAGCTCAACGGCCAAGGAGCCACCGCCGACGCCATTGCTCTTCTTGGCGCCCTTGTTATGCCGCATAACCTCTTTCTTCATTCGGCTCTCGTGCTGTCAAGGAAAGTCCCAAATTCCGTCCGTGGTATCAACGATGCGTGTAGATACTTCCTAATAGAGAGTGGATTTGCATTGTTCATAGCATTTTTAATAAACGTGGCCGTCGTTTCAGTTTCCGGCACCGTTTGTACGGTCGGAAATGTCTCCGAAACCACCGCCGATCAATGCTCCGACATAACTCTCAATTCCGCCTCCTTCCTCCTCCAGAACGTTTTGGGAAAATCAAGCTCCATTATTTACGCCATTGCTTTGTTTGCCTCTGGACAAAGCTCCAGCATCACCGGCACTTATGCCGGTCAGTTCATCATGCAGGGATTTTTGGACCTGAAAATGAAAACATGGGCTCGAAACCTTATGACAAGGTCCATTGCAATAACGCCTAGTCTCATCGTATCCATCATCGGTGGACCTCAAGGAGCTGGCCGTCTCATCATTATTGCATCGATGATTCTTTCCTTCGAGCTTCCATTTGCTCTAATTCCACTCCTCAAATTCAGTAGCAGCTCCACCAAGATGGGCCCCCACAAGAATTCCATATACATAATTGTAATCTCGTGGATATTGGGCCTGGCAATCATCGGCATCAACATCTATTATCTCAGTACGGCCTTTGTGGGCTGGCTAATCCATAACAATTTACACAGAATTGCAAATGTGTTCATTGGGATCGTTGTTTTTCCATTAATGGCCATCTACGTTCTTGCCGTTATTTACCTTATGTTTCGAAAAGACAGGGTTGTCACTTACATTGAGCCCGAAAAGGATGACCCCATGGACCAAGCCCATTTGGAAAGTGGGCTTAATAATTCTTTTGGGCCGCGTGACGTCACTCCGGTGCCTTTCAGAGAGGACTTGGCCCATATCCCACTACCAGAGTAG

Protein sequence

MGSQQMEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTVIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRIANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPRDVTPVPFREDLAHIPLPE
Homology
BLAST of Lsi08G004550 vs. ExPASy Swiss-Prot
Match: Q8H4H5 (Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 4.4e-185
Identity = 342/503 (67.99%), Postives = 401/503 (79.72%), Query Frame = 0

Query: 55  QKPGWKKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLA 114
           ++P WK+FL+HVGPGF+VSLAYLDPGNLETDLQAGANH +ELLWV+LIGLIFALIIQSLA
Sbjct: 37  KEPAWKRFLAHVGPGFMVSLAYLDPGNLETDLQAGANHRYELLWVILIGLIFALIIQSLA 96

Query: 115 ANLGVST-----------------------------------VIGTAFALNILFHIPVWA 174
           ANLGV T                                   VIGTAFA NILFHIPVW 
Sbjct: 97  ANLGVVTGRHLAEICKSEYPKFVKIFLWLLAELAVIAADIPEVIGTAFAFNILFHIPVWV 156

Query: 175 GVLLTGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPK 234
           GVL+TG STLLLLGLQ+YG RKLE LI++LVF+MAACFFGE+S VKPPA  V+KG+F+P+
Sbjct: 157 GVLITGTSTLLLLGLQKYGVRKLEFLISMLVFVMAACFFGELSIVKPPAKEVMKGLFIPR 216

Query: 235 LNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIA 294
           LNG GATADAIALLGALVMPHNLFLHSALVLSRK P SVRGI D CR+FL ESGFALF+A
Sbjct: 217 LNGDGATADAIALLGALVMPHNLFLHSALVLSRKTPASVRGIKDGCRFFLYESGFALFVA 276

Query: 295 FLINVAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASG 354
            LIN+AVVSVSGT C+  N+S+  AD+C++++L+++SFLL+NVLGKSS+I+Y +AL ASG
Sbjct: 277 LLINIAVVSVSGTACSSANLSQEDADKCANLSLDTSSFLLKNVLGKSSAIVYGVALLASG 336

Query: 355 QSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIAS 414
           QSS+ITGTYAGQ+IMQGFLD++M+ W RNLMTR+IAI PSLIVSIIGG +GAGRLIIIAS
Sbjct: 337 QSSTITGTYAGQYIMQGFLDIRMRKWLRNLMTRTIAIAPSLIVSIIGGSRGAGRLIIIAS 396

Query: 415 MILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWL 474
           MILSFELPFALIPLLKFSSS +KMGPHKNSIYIIV SW LGL IIGIN+Y+LST+FVGWL
Sbjct: 397 MILSFELPFALIPLLKFSSSKSKMGPHKNSIYIIVFSWFLGLLIIGINMYFLSTSFVGWL 456

Query: 475 IHNNLHRIANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLN 523
           IHN+L + ANV +G  VFP M +Y++AV+YL  RKD VVT++       +  A  E    
Sbjct: 457 IHNDLPKYANVLVGAAVFPFMLVYIVAVVYLTIRKDSVVTFVADSSLAAVVDA--EKADA 516

BLAST of Lsi08G004550 vs. ExPASy Swiss-Prot
Match: Q0D7E4 (Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 4.0e-170
Identity = 316/477 (66.25%), Postives = 374/477 (78.41%), Query Frame = 0

Query: 55  QKPGWKKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLA 114
           ++P WK+FLSH+GPGF+V LAYLDPGN+ETDLQAGANH +ELLWV+LIGLIFALIIQSL+
Sbjct: 28  KEPAWKRFLSHIGPGFMVCLAYLDPGNMETDLQAGANHKYELLWVILIGLIFALIIQSLS 87

Query: 115 ANLGVST-----------------------------------VIGTAFALNILFHIPVWA 174
           ANLGV T                                   VIGT FA N+LFHIPVW 
Sbjct: 88  ANLGVVTGRHLAELCKTEYPVWVKTCLWLLAELAVIASDIPEVIGTGFAFNLLFHIPVWT 147

Query: 175 GVLLTGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPK 234
           GVL+ G STLLLLGLQRYG RKLE+++A+LVF+MA CFF EMS VKPP   VL+G+F+P+
Sbjct: 148 GVLIAGSSTLLLLGLQRYGVRKLEVVVALLVFVMAGCFFVEMSIVKPPVNEVLQGLFIPR 207

Query: 235 LNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIA 294
           L+G GAT D+IALLGALVMPHNLFLHSALVLSR  P S +G+ D CR+FL ESG ALF+A
Sbjct: 208 LSGPGATGDSIALLGALVMPHNLFLHSALVLSRNTPASAKGMKDVCRFFLFESGIALFVA 267

Query: 295 FLINVAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASG 354
            L+N+A++SVSGTVC   N+S   A +CSD+TL+S+SFLL+NVLGKSS+ +Y +AL ASG
Sbjct: 268 LLVNIAIISVSGTVCNATNLSPEDAVKCSDLTLDSSSFLLRNVLGKSSATVYGVALLASG 327

Query: 355 QSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIAS 414
           QSS+ITGTYAGQ++MQGFLD+KMK W RNLMTRSIAI PSLIVSIIGG  GAGRLI+IAS
Sbjct: 328 QSSTITGTYAGQYVMQGFLDIKMKQWLRNLMTRSIAIVPSLIVSIIGGSSGAGRLIVIAS 387

Query: 415 MILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWL 474
           MILSFELPFALIPLLKFSSSS KMG +KNSIYI+  SW+LG  IIGINIY+LST  VGW+
Sbjct: 388 MILSFELPFALIPLLKFSSSSNKMGENKNSIYIVGFSWVLGFVIIGINIYFLSTKLVGWI 447

Query: 475 IHNNLHRIANVFIGIVVFPLMAIYVLAVIYLMFRKDRV--VTYIEPEKDDPMDQAHL 495
           +HN L   ANV IGIV+FPLM +YV+AVIYL FRKD V  V+  E +  D  ++A +
Sbjct: 448 LHNALPTFANVLIGIVLFPLMLLYVVAVIYLTFRKDTVKFVSRRELQAGDDTEKAQV 504

BLAST of Lsi08G004550 vs. ExPASy Swiss-Prot
Match: Q6ZG85 (Metal transporter NRAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAT1 PE=2 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 8.4e-152
Identity = 289/501 (57.68%), Postives = 367/501 (73.25%), Query Frame = 0

Query: 56  KPGWKKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAA 115
           +P W+KFL+HVGPG LV++ +LDP NLETD+QAGA+  +ELLWV+L+G++FAL+IQ+LAA
Sbjct: 45  QPRWRKFLAHVGPGALVAIGFLDPSNLETDMQAGADFKYELLWVILVGMVFALLIQTLAA 104

Query: 116 NLGVST-----------------------------------VIGTAFALNILFHIPVWAG 175
           NLGV T                                   V+GTAFA NIL  IPVWAG
Sbjct: 105 NLGVKTGRHLAELCREEYPHYVNIFLWIIAELAVISDDIPEVLGTAFAFNILLKIPVWAG 164

Query: 176 VLLTGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKL 235
           V+LT  STLLLLG+QR+GARKLE +IA  +F MAACFFGE+SY++P A  V+KGMFVP L
Sbjct: 165 VILTVFSTLLLLGVQRFGARKLEFIIAAFMFTMAACFFGELSYLRPSAGEVVKGMFVPSL 224

Query: 236 NGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAF 295
            G+GA A+AIAL GA++ P+NLFLHSALVLSRK P S + I  ACRYFLIE   A  +AF
Sbjct: 225 QGKGAAANAIALFGAIITPYNLFLHSALVLSRKTPRSDKSIRAACRYFLIECSLAFIVAF 284

Query: 296 LINVAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQ 355
           LINV+VV V+G++C   N+S   A+ C D+TL S   LL+NVLG+SSS++YA+AL ASGQ
Sbjct: 285 LINVSVVVVAGSICNANNLSPADANTCGDLTLQSTPLLLRNVLGRSSSVVYAVALLASGQ 344

Query: 356 SSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASM 415
           S++I+ T+AGQ IMQGFLD+KMK W RNL+TR IAI PSLIVSI+ GP GAG+LII++SM
Sbjct: 345 STTISCTFAGQVIMQGFLDMKMKNWVRNLITRVIAIAPSLIVSIVSGPSGAGKLIILSSM 404

Query: 416 ILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLI 475
           ILSFELPFALIPLLKF +SS K+GP K SIY +VI+WIL  A+I +N Y+L   +V WL+
Sbjct: 405 ILSFELPFALIPLLKFCNSSKKVGPLKESIYTVVIAWILSFALIVVNTYFLVWTYVDWLV 464

Query: 476 HNNLHRIANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIE-PEKDDPMDQAHLESG-- 518
           HNNL + AN  I +VVF LMA Y++AV+YL FRKD V TY+  PE+     QA +E+G  
Sbjct: 465 HNNLPKYANGLISVVVFALMAAYLVAVVYLTFRKDTVATYVPVPER----AQAQVEAGGT 524

BLAST of Lsi08G004550 vs. ExPASy Swiss-Prot
Match: Q9SAH8 (Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.2e-144
Identity = 282/501 (56.29%), Postives = 359/501 (71.66%), Query Frame = 0

Query: 21  GSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKFLSHVGPGFLVSLAYLDPG 80
           GS R   ++++    + +N+   +N    Q    +K  WK F +++GPGFLVS+AY+DPG
Sbjct: 5   GSGRSQFISSSGGNRSFSNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSIAYIDPG 64

Query: 81  NLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST------------------- 140
           N ETDLQAGA++ +ELLW++L+    AL+IQSLAANLGV T                   
Sbjct: 65  NFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYSKVPNFM 124

Query: 141 ----------------VIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELL 200
                           VIGTAFALN+LF IPVW GVLLTGLSTL+LL LQ+YG RKLE L
Sbjct: 125 LWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGVRKLEFL 184

Query: 201 IAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLH 260
           IA LVF +A CFF E+ Y KP    VL G+FVP+L G GAT  AI+LLGA+VMPHNLFLH
Sbjct: 185 IAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMPHNLFLH 244

Query: 261 SALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVVSVSGTVCTVGNVSETTAD 320
           SALVLSRK+P S  GI +ACR++LIESG AL +AFLINV+V+SVSG VC   N+S     
Sbjct: 245 SALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNLSPEDRA 304

Query: 321 QCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTW 380
            C D+ LN ASFLL+NV+GK SS ++AIAL ASGQSS+ITGTYAGQ++MQGFLDL+++ W
Sbjct: 305 NCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPW 364

Query: 381 ARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGP 440
            RNL+TR +AI PSLIV++IGG  GAG+LIIIASMILSFELPFAL+PLLKF+S  TKMG 
Sbjct: 365 LRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSCKTKMGS 424

Query: 441 HKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRIANVFIGIVVFPLMAIYVL 487
           H N + I  ++W++G  I+GINIYYL ++F+  LIH+++  I  VF GI+ F  +A+Y+ 
Sbjct: 425 HVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAGIALYLA 484

BLAST of Lsi08G004550 vs. ExPASy Swiss-Prot
Match: Q9S9N8 (Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2)

HSP 1 Score: 510.8 bits (1314), Expect = 1.9e-143
Identity = 278/487 (57.08%), Postives = 354/487 (72.69%), Query Frame = 0

Query: 25  IAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKFLSHVGPGFLVSLAYLDPGNLET 84
           +AA   +    + +N+   +N    Q    +K  WK F S++GPGFLVS+AY+DPGN ET
Sbjct: 1   MAAETASGSNRSISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFET 60

Query: 85  DLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST----------------------- 144
           DLQ+GA + +ELLW++L+    AL+IQSLAANLGV T                       
Sbjct: 61  DLQSGAQYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVV 120

Query: 145 ------------VIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAIL 204
                       VIGTAFALN+LF+IPVW GVLLTGLSTL+LL LQ+YG RKLE LIA L
Sbjct: 121 AEIAVVACDIPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFL 180

Query: 205 VFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALV 264
           VF +A CFF E+ Y KP    VL G+FVP+L G GAT  AI+LLGA+VMPHNLFLHSALV
Sbjct: 181 VFTIALCFFVELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALV 240

Query: 265 LSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVVSVSGTVCTVGNVSETTADQCSD 324
           LSRK+P SV GI +ACRY+LIESG AL +AFLINV+V+SVSG VC   ++S      C D
Sbjct: 241 LSRKIPRSVTGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQD 300

Query: 325 ITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNL 384
           + LN ASFLL+NV+GK SS ++AIAL ASGQSS+ITGTYAGQ++MQGFLDL+++ W RN 
Sbjct: 301 LDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNF 360

Query: 385 MTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNS 444
           +TR +AI PSLIV++IGG  GAG+LIIIASMILSFELPFAL+PLLKF+SS TKMG H NS
Sbjct: 361 LTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANS 420

Query: 445 IYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRIANVFIGIVVFPLMAIYVLAVIY 477
           + I  ++WI+G  I+GINIYYL ++F+  L+H++++ +A VF+G++ F  +A Y+ A+ Y
Sbjct: 421 LVISSVTWIIGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISY 480

BLAST of Lsi08G004550 vs. ExPASy TrEMBL
Match: A0A0A0KDB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G382880 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 6.3e-259
Identity = 487/553 (88.07%), Postives = 501/553 (90.60%), Query Frame = 0

Query: 6   MEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKFLSH 65
           MEAQMSTAATPSFGGGSNRIAAVN     TTPTNA+KPQNHQE + TTHQKPGW+KFLS+
Sbjct: 1   MEAQMSTAATPSFGGGSNRIAAVN----ITTPTNALKPQNHQEGKNTTHQKPGWRKFLSY 60

Query: 66  VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST---- 125
           VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST    
Sbjct: 61  VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTGKHL 120

Query: 126 -------------------------------VIGTAFALNILFHIPVWAGVLLTGLSTLL 185
                                          VIGTAFALNILFHIPVWAGVLLTGLSTLL
Sbjct: 121 SEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLL 180

Query: 186 LLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAI 245
           LLGLQRYGARKLELLIAILVF+MAACFFGEMSYVKPPA+GVLKGMFVPKL G+GATADAI
Sbjct: 181 LLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLKGEGATADAI 240

Query: 246 ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVVSVS 305
           ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESG ALFIAFLINVAVVSVS
Sbjct: 241 ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAFLINVAVVSVS 300

Query: 306 GTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGTYAG 365
           GTVCTVGNV++TTADQCSDITLNSASFLLQNVLGKSSS IYAIALFASGQSSSITGTYAG
Sbjct: 301 GTVCTVGNVTQTTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSITGTYAG 360

Query: 366 QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL 425
           QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL
Sbjct: 361 QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL 420

Query: 426 IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRIANV 485
           IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFV WLIHNNL ++ANV
Sbjct: 421 IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLIHNNLPKVANV 480

Query: 486 FIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPRDVTPV 524
           FIGI VFPLMA Y+ AVIYLMFRKDRVVTYIEPEKDD + QAHLESGLNNSFGPRDVTP+
Sbjct: 481 FIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAVAQAHLESGLNNSFGPRDVTPL 540

BLAST of Lsi08G004550 vs. ExPASy TrEMBL
Match: A0A6J1GNU9 (metal transporter Nramp5-like OS=Cucurbita moschata OX=3662 GN=LOC111455783 PE=3 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 1.0e-256
Identity = 480/556 (86.33%), Postives = 499/556 (89.75%), Query Frame = 0

Query: 3   SQQMEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKF 62
           SQQ EAQMST ATPSFGGGSNRIAAVNT E TT P +AVK QNH+EE TTT QKPGW+KF
Sbjct: 4   SQQKEAQMSTVATPSFGGGSNRIAAVNTAETTTIPADAVKTQNHREEPTTTDQKPGWRKF 63

Query: 63  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST- 122
           LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST 
Sbjct: 64  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTG 123

Query: 123 ----------------------------------VIGTAFALNILFHIPVWAGVLLTGLS 182
                                             VIGTAFALNILFHIPVWAGVLLTGLS
Sbjct: 124 KHLSEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLS 183

Query: 183 TLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATA 242
           TLLL+GLQRYG RKLELLIAILVF+MAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 
Sbjct: 184 TLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATG 243

Query: 243 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVV 302
           DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFI+FLIN+AVV
Sbjct: 244 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAVV 303

Query: 303 SVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGT 362
           SVSGTVCTVG+VSE+TADQCSDITLNSASFLLQNVLGKSSS IYAIAL ASGQSSSITGT
Sbjct: 304 SVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGT 363

Query: 363 YAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELP 422
           YAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIASMILSFELP
Sbjct: 364 YAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFELP 423

Query: 423 FALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRI 482
           FALIPLLKFSSS+TKMGPHKNSIYIIVISWILGL+IIGINIYYLST FVGWLI NNL ++
Sbjct: 424 FALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTGFVGWLIDNNLPKV 483

Query: 483 ANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPRDV 524
           ANVFIGIVVFPLMA+Y+LAVIYL FRKDRVVTYIEP+K DPM QAHLESGL+NSFGP DV
Sbjct: 484 ANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGDV 543

BLAST of Lsi08G004550 vs. ExPASy TrEMBL
Match: A0A1S3CA24 (metal transporter Nramp5-like OS=Cucumis melo OX=3656 GN=LOC103498358 PE=3 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 6.5e-256
Identity = 482/553 (87.16%), Postives = 494/553 (89.33%), Query Frame = 0

Query: 6   MEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKFLSH 65
           ME QMST  TPS GGGSNRIAAVN     TTP NAV  QNHQEE+ TTHQKPGW+KF+SH
Sbjct: 1   MEVQMSTTVTPSLGGGSNRIAAVN----ITTPINAVNLQNHQEEKNTTHQKPGWRKFVSH 60

Query: 66  VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST---- 125
           VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST    
Sbjct: 61  VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTGKHL 120

Query: 126 -------------------------------VIGTAFALNILFHIPVWAGVLLTGLSTLL 185
                                          VIGTAFALNILFHIPVWAGVLLTGLSTLL
Sbjct: 121 SEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLL 180

Query: 186 LLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAI 245
           LLGLQRYGARKLELLIAILVF+MAACFFGEMSYVKPPA+ VLKGMFVPKL G+GATADAI
Sbjct: 181 LLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASSVLKGMFVPKLKGEGATADAI 240

Query: 246 ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVVSVS 305
           ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESG ALFIAFLINVAVVSVS
Sbjct: 241 ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAFLINVAVVSVS 300

Query: 306 GTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGTYAG 365
           GTVCTVGNV++TTADQCSDITLNSASFLLQNVLGKSSS IYAIALFASGQSSSITGTYAG
Sbjct: 301 GTVCTVGNVAQTTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSITGTYAG 360

Query: 366 QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL 425
           QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL
Sbjct: 361 QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL 420

Query: 426 IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRIANV 485
           IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFV WLIHNNLH++ANV
Sbjct: 421 IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLIHNNLHKVANV 480

Query: 486 FIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPRDVTPV 524
           FIGI VFPLMA Y+ AVIYLMFRKDRVVTYIEPEKDD M QAHLE+GLNNSFGPRDVTPV
Sbjct: 481 FIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAMAQAHLETGLNNSFGPRDVTPV 540

BLAST of Lsi08G004550 vs. ExPASy TrEMBL
Match: A0A6J1JPX4 (metal transporter Nramp5-like OS=Cucurbita maxima OX=3661 GN=LOC111487830 PE=3 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 7.2e-255
Identity = 477/556 (85.79%), Postives = 498/556 (89.57%), Query Frame = 0

Query: 3   SQQMEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKF 62
           SQQ E+QMS  ATPSFG GSNRIAAVNT + TT P +AVKPQNH+E  TTT QKPGW+KF
Sbjct: 4   SQQKESQMSIVATPSFGSGSNRIAAVNTAKITTIPADAVKPQNHREVPTTTDQKPGWRKF 63

Query: 63  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST- 122
           LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST 
Sbjct: 64  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTG 123

Query: 123 ----------------------------------VIGTAFALNILFHIPVWAGVLLTGLS 182
                                             VIGTAFALNILFHIPVWAGVLLTGLS
Sbjct: 124 KHLSEVCRAEYPKLVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLS 183

Query: 183 TLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATA 242
           TLLL+GLQRYG RKLELLIAILVF+MAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 
Sbjct: 184 TLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATG 243

Query: 243 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVV 302
           DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFI+FLIN+AVV
Sbjct: 244 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAVV 303

Query: 303 SVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGT 362
           SVSGTVCTVG+VSE+TADQCSDITLNSASFLLQNVLGKSSS IYAIAL ASGQSSSITGT
Sbjct: 304 SVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGT 363

Query: 363 YAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELP 422
           YAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIASMILSFELP
Sbjct: 364 YAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFELP 423

Query: 423 FALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRI 482
           FALIPLLKFSSS+TKMGPHKNSIYIIVISWILGL+IIGINIYYLSTAFVGWLI NNL ++
Sbjct: 424 FALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTAFVGWLIDNNLPKV 483

Query: 483 ANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPRDV 524
           ANVFIGIVVFPLMA+Y+LAVIYL FRKDRVVTYIEP+K DPM QAHLESGL+NSFGP DV
Sbjct: 484 ANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGDV 543

BLAST of Lsi08G004550 vs. ExPASy TrEMBL
Match: A0A6J1CJ54 (metal transporter Nramp5-like OS=Momordica charantia OX=3673 GN=LOC111011923 PE=3 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.6e-241
Identity = 463/560 (82.68%), Postives = 483/560 (86.25%), Query Frame = 0

Query: 1   MGSQQMEAQM--STAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPG 60
           MG+QQ EAQM  +TAA PSFG GSNRIAA    E  T P  A K            +KPG
Sbjct: 3   MGTQQKEAQMITTTAAAPSFGSGSNRIAA----ETMTPPATAAK----------KIEKPG 62

Query: 61  WKKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLG 120
           W+KFLSHVGPGFLVSLAYLDPGNLETDLQAGA+HG+ELLWVVLIGLIFALIIQSLAANLG
Sbjct: 63  WRKFLSHVGPGFLVSLAYLDPGNLETDLQAGASHGYELLWVVLIGLIFALIIQSLAANLG 122

Query: 121 VST-----------------------------------VIGTAFALNILFHIPVWAGVLL 180
           VST                                   V+GTAFALNILFHIPVWAGVLL
Sbjct: 123 VSTGKHLSEVCKAEYPKIVKYCLWLLAEVAVIAADIPEVVGTAFALNILFHIPVWAGVLL 182

Query: 181 TGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQ 240
           TGLSTLLLLGLQRYGARKLELLIAILVF+MAACFFGEMSYVKPPA+GVLKGMFVPKLNGQ
Sbjct: 183 TGLSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLNGQ 242

Query: 241 GATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLIN 300
           GAT DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALF+AFLIN
Sbjct: 243 GATGDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFVAFLIN 302

Query: 301 VAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSS 360
           VAVVSVSGTVCTVGNVS  TADQC DITLNSASFLLQNVLGKSSS IYAIAL ASGQSSS
Sbjct: 303 VAVVSVSGTVCTVGNVSPATADQCGDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSS 362

Query: 361 ITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILS 420
           ITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAI PSL+VSIIGG QGAGRLIIIASMILS
Sbjct: 363 ITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAIAPSLVVSIIGGSQGAGRLIIIASMILS 422

Query: 421 FELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNN 480
           FELPFALIPLLKFSSS+TKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIH++
Sbjct: 423 FELPFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHSD 482

Query: 481 LHRIANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFG 524
           L ++ANV IGIVVFPLMA+Y+LAVIYL FRKDRVVTYIEPEK DPM QAH+ESGL+NSFG
Sbjct: 483 LPKVANVLIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPEKHDPMAQAHMESGLSNSFG 542

BLAST of Lsi08G004550 vs. NCBI nr
Match: XP_038886296.1 (metal transporter Nramp5-like [Benincasa hispida])

HSP 1 Score: 937.6 bits (2422), Expect = 4.7e-269
Identity = 504/558 (90.32%), Postives = 509/558 (91.22%), Query Frame = 0

Query: 1   MGSQQMEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWK 60
           MGSQQ EAQMST ATPSFGGGSNRIAAVNT   T TPTN VKPQNH+EEQT  HQKPGW+
Sbjct: 1   MGSQQKEAQMSTVATPSFGGGSNRIAAVNTGVATATPTNTVKPQNHREEQTINHQKPGWR 60

Query: 61  KFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVS 120
           KFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVS
Sbjct: 61  KFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVS 120

Query: 121 T-----------------------------------VIGTAFALNILFHIPVWAGVLLTG 180
           T                                   VIGTAFALNILFHIPVWAGVLLTG
Sbjct: 121 TGKHLSEVCKAEYPIFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTG 180

Query: 181 LSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGA 240
           LSTLLLLGLQRYGARKLELLIAILVF+MAACFFGEMSYVKPPATGVLKGMFVPKLNGQGA
Sbjct: 181 LSTLLLLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGA 240

Query: 241 TADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVA 300
           TADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGIN+ACRYFLIESGFALFIAFLINVA
Sbjct: 241 TADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINEACRYFLIESGFALFIAFLINVA 300

Query: 301 VVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSIT 360
           VVSVSGTVCTVG VSETTADQCSDITLNSASFLLQNVLGKSSS IYAIALFASGQSSSIT
Sbjct: 301 VVSVSGTVCTVGKVSETTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSIT 360

Query: 361 GTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFE 420
           GTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFE
Sbjct: 361 GTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFE 420

Query: 421 LPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLH 480
           LPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLH
Sbjct: 421 LPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLH 480

Query: 481 RIANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPR 524
           RIANVFIGIVVFPLMA Y+LAVIYLMFRKDRVVTYIEPEKDDPM QAHLESGLNNSFGPR
Sbjct: 481 RIANVFIGIVVFPLMAAYILAVIYLMFRKDRVVTYIEPEKDDPMAQAHLESGLNNSFGPR 540

BLAST of Lsi08G004550 vs. NCBI nr
Match: KAG7032815.1 (Metal transporter Nramp5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 913.3 bits (2359), Expect = 9.6e-262
Identity = 480/521 (92.13%), Postives = 499/521 (95.78%), Query Frame = 0

Query: 3   SQQMEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKF 62
           SQQ EAQMST ATPSFGGGSNRIAAVNT E TT P +AVK QNH+EE TTT QKPGW+KF
Sbjct: 4   SQQKEAQMSTVATPSFGGGSNRIAAVNTAETTTIPADAVKTQNHREEPTTTDQKPGWRKF 63

Query: 63  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTV 122
           LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTV
Sbjct: 64  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTV 123

Query: 123 IGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMS 182
           IGTAFALNILFHIPVWAGVLLTGLSTLLL+GLQRYG RKLELLIAILVF+MAACFFGEMS
Sbjct: 124 IGTAFALNILFHIPVWAGVLLTGLSTLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMS 183

Query: 183 YVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVRGIN 242
           YVKPPATGVLKGMFVPKLNGQGAT DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGIN
Sbjct: 184 YVKPPATGVLKGMFVPKLNGQGATGDAIALLGALVMPHNLFLHSALVLSRKVPNSVRGIN 243

Query: 243 DACRYFLIESGFALFIAFLINVAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNV 302
           DACRYFLIESGFALFI+FLIN+AVVSVSGTVCTVG+VSE+TADQCSDITLNSASFLLQNV
Sbjct: 244 DACRYFLIESGFALFISFLINMAVVSVSGTVCTVGHVSESTADQCSDITLNSASFLLQNV 303

Query: 303 LGKSSSIIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIV 362
           LGKSSS IYAIAL ASGQSSSITGTYAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV
Sbjct: 304 LGKSSSTIYAIALLASGQSSSITGTYAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIV 363

Query: 363 SIIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLA 422
           +IIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSS+TKMGPHKNSIYIIVISWILGL+
Sbjct: 364 AIIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLS 423

Query: 423 IIGINIYYLSTAFVGWLIHNNLHRIANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIE 482
           IIGINIYYLST FVGWLI NNL ++ANVFIGIVVFPLMA+Y+LAVIYL FRKDRVVTYIE
Sbjct: 424 IIGINIYYLSTGFVGWLIDNNLPKVANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIE 483

Query: 483 PEKDDPMDQAHLESGLNNSFGPRDVTPVPFREDLAHIPLPE 524
           P+K DPM QAHLESGL+NSFGP DV PVPFR+DLAHIPLPE
Sbjct: 484 PQKPDPMAQAHLESGLHNSFGPGDVAPVPFRDDLAHIPLPE 524

BLAST of Lsi08G004550 vs. NCBI nr
Match: XP_011658448.2 (metal transporter Nramp5 [Cucumis sativus] >KGN47700.1 hypothetical protein Csa_018232 [Cucumis sativus])

HSP 1 Score: 902.9 bits (2332), Expect = 1.3e-258
Identity = 487/553 (88.07%), Postives = 501/553 (90.60%), Query Frame = 0

Query: 6   MEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKFLSH 65
           MEAQMSTAATPSFGGGSNRIAAVN     TTPTNA+KPQNHQE + TTHQKPGW+KFLS+
Sbjct: 1   MEAQMSTAATPSFGGGSNRIAAVN----ITTPTNALKPQNHQEGKNTTHQKPGWRKFLSY 60

Query: 66  VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST---- 125
           VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST    
Sbjct: 61  VGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTGKHL 120

Query: 126 -------------------------------VIGTAFALNILFHIPVWAGVLLTGLSTLL 185
                                          VIGTAFALNILFHIPVWAGVLLTGLSTLL
Sbjct: 121 SEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLSTLL 180

Query: 186 LLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAI 245
           LLGLQRYGARKLELLIAILVF+MAACFFGEMSYVKPPA+GVLKGMFVPKL G+GATADAI
Sbjct: 181 LLGLQRYGARKLELLIAILVFVMAACFFGEMSYVKPPASGVLKGMFVPKLKGEGATADAI 240

Query: 246 ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVVSVS 305
           ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESG ALFIAFLINVAVVSVS
Sbjct: 241 ALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGIALFIAFLINVAVVSVS 300

Query: 306 GTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGTYAG 365
           GTVCTVGNV++TTADQCSDITLNSASFLLQNVLGKSSS IYAIALFASGQSSSITGTYAG
Sbjct: 301 GTVCTVGNVTQTTADQCSDITLNSASFLLQNVLGKSSSTIYAIALFASGQSSSITGTYAG 360

Query: 366 QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL 425
           QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL
Sbjct: 361 QFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFAL 420

Query: 426 IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRIANV 485
           IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFV WLIHNNL ++ANV
Sbjct: 421 IPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVDWLIHNNLPKVANV 480

Query: 486 FIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPRDVTPV 524
           FIGI VFPLMA Y+ AVIYLMFRKDRVVTYIEPEKDD + QAHLESGLNNSFGPRDVTP+
Sbjct: 481 FIGIAVFPLMAAYIGAVIYLMFRKDRVVTYIEPEKDDAVAQAHLESGLNNSFGPRDVTPL 540

BLAST of Lsi08G004550 vs. NCBI nr
Match: XP_023526032.1 (metal transporter Nramp5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 898.3 bits (2320), Expect = 3.2e-257
Identity = 480/556 (86.33%), Postives = 500/556 (89.93%), Query Frame = 0

Query: 3   SQQMEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKF 62
           SQQ EAQMST ATPSFGGGSNRIAAVNT E TT P +AVKPQNH+EE TTT QKPGW+KF
Sbjct: 4   SQQKEAQMSTVATPSFGGGSNRIAAVNTAETTTIPADAVKPQNHREEPTTTDQKPGWRKF 63

Query: 63  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST- 122
           LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVL+GLIFALIIQSLAANLGVST 
Sbjct: 64  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLVGLIFALIIQSLAANLGVSTG 123

Query: 123 ----------------------------------VIGTAFALNILFHIPVWAGVLLTGLS 182
                                             VIGTAFALNILFHIPVWAGVLLTGLS
Sbjct: 124 KHLSEVCKAEYPKLVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLS 183

Query: 183 TLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATA 242
           TLLL+GLQRYG RKLELLIAILVF+MAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 
Sbjct: 184 TLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATG 243

Query: 243 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVV 302
           DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFI+FLIN+AVV
Sbjct: 244 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAVV 303

Query: 303 SVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGT 362
           SVSGTVCTVG+VSE+TADQCSDITLNSASFLLQNVLGKSSS IYAIAL ASGQSSSITGT
Sbjct: 304 SVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGT 363

Query: 363 YAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELP 422
           YAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIASMILSFELP
Sbjct: 364 YAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFELP 423

Query: 423 FALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRI 482
           FALIPLLKFSSS+TKMGPHKNSIYIIVISWILGL+IIGINIYYLST FVGWLI NNL ++
Sbjct: 424 FALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTGFVGWLIDNNLPKV 483

Query: 483 ANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPRDV 524
           ANVFIGIVVFPLMA+Y+LAVIYL FRKDRVVTYIEP+K DPM QAHLESGL+NSFGP DV
Sbjct: 484 ANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGDV 543

BLAST of Lsi08G004550 vs. NCBI nr
Match: XP_022953164.1 (metal transporter Nramp5-like [Cucurbita moschata])

HSP 1 Score: 895.6 bits (2313), Expect = 2.1e-256
Identity = 480/556 (86.33%), Postives = 499/556 (89.75%), Query Frame = 0

Query: 3   SQQMEAQMSTAATPSFGGGSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKF 62
           SQQ EAQMST ATPSFGGGSNRIAAVNT E TT P +AVK QNH+EE TTT QKPGW+KF
Sbjct: 4   SQQKEAQMSTVATPSFGGGSNRIAAVNTAETTTIPADAVKTQNHREEPTTTDQKPGWRKF 63

Query: 63  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST- 122
           LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST 
Sbjct: 64  LSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVSTG 123

Query: 123 ----------------------------------VIGTAFALNILFHIPVWAGVLLTGLS 182
                                             VIGTAFALNILFHIPVWAGVLLTGLS
Sbjct: 124 KHLSEVCKAEYPKFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFHIPVWAGVLLTGLS 183

Query: 183 TLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATA 242
           TLLL+GLQRYG RKLELLIAILVF+MAACFFGEMSYVKPPATGVLKGMFVPKLNGQGAT 
Sbjct: 184 TLLLIGLQRYGVRKLELLIAILVFVMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATG 243

Query: 243 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVV 302
           DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFI+FLIN+AVV
Sbjct: 244 DAIALLGALVMPHNLFLHSALVLSRKVPNSVRGINDACRYFLIESGFALFISFLINMAVV 303

Query: 303 SVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGT 362
           SVSGTVCTVG+VSE+TADQCSDITLNSASFLLQNVLGKSSS IYAIAL ASGQSSSITGT
Sbjct: 304 SVSGTVCTVGHVSESTADQCSDITLNSASFLLQNVLGKSSSTIYAIALLASGQSSSITGT 363

Query: 363 YAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELP 422
           YAGQFIMQGFLDLKMKTW RNLMTRSIAITPSLIV+IIGGPQGAGRLIIIASMILSFELP
Sbjct: 364 YAGQFIMQGFLDLKMKTWMRNLMTRSIAITPSLIVAIIGGPQGAGRLIIIASMILSFELP 423

Query: 423 FALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRI 482
           FALIPLLKFSSS+TKMGPHKNSIYIIVISWILGL+IIGINIYYLST FVGWLI NNL ++
Sbjct: 424 FALIPLLKFSSSTTKMGPHKNSIYIIVISWILGLSIIGINIYYLSTGFVGWLIDNNLPKV 483

Query: 483 ANVFIGIVVFPLMAIYVLAVIYLMFRKDRVVTYIEPEKDDPMDQAHLESGLNNSFGPRDV 524
           ANVFIGIVVFPLMA+Y+LAVIYL FRKDRVVTYIEP+K DPM QAHLESGL+NSFGP DV
Sbjct: 484 ANVFIGIVVFPLMAVYILAVIYLTFRKDRVVTYIEPQKPDPMAQAHLESGLHNSFGPGDV 543

BLAST of Lsi08G004550 vs. TAIR 10
Match: AT1G80830.1 (natural resistance-associated macrophage protein 1 )

HSP 1 Score: 513.8 bits (1322), Expect = 1.6e-145
Identity = 282/501 (56.29%), Postives = 359/501 (71.66%), Query Frame = 0

Query: 21  GSNRIAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKFLSHVGPGFLVSLAYLDPG 80
           GS R   ++++    + +N+   +N    Q    +K  WK F +++GPGFLVS+AY+DPG
Sbjct: 5   GSGRSQFISSSGGNRSFSNSPLIENSDSNQIIVSEKKSWKNFFAYLGPGFLVSIAYIDPG 64

Query: 81  NLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST------------------- 140
           N ETDLQAGA++ +ELLW++L+    AL+IQSLAANLGV T                   
Sbjct: 65  NFETDLQAGAHYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEQCRAEYSKVPNFM 124

Query: 141 ----------------VIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELL 200
                           VIGTAFALN+LF IPVW GVLLTGLSTL+LL LQ+YG RKLE L
Sbjct: 125 LWVVAEIAVVACDIPEVIGTAFALNMLFSIPVWIGVLLTGLSTLILLALQKYGVRKLEFL 184

Query: 201 IAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLH 260
           IA LVF +A CFF E+ Y KP    VL G+FVP+L G GAT  AI+LLGA+VMPHNLFLH
Sbjct: 185 IAFLVFTIAICFFVELHYSKPDPGEVLHGLFVPQLKGNGATGLAISLLGAMVMPHNLFLH 244

Query: 261 SALVLSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVVSVSGTVCTVGNVSETTAD 320
           SALVLSRK+P S  GI +ACR++LIESG AL +AFLINV+V+SVSG VC   N+S     
Sbjct: 245 SALVLSRKIPRSASGIKEACRFYLIESGLALMVAFLINVSVISVSGAVCNAPNLSPEDRA 304

Query: 321 QCSDITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTW 380
            C D+ LN ASFLL+NV+GK SS ++AIAL ASGQSS+ITGTYAGQ++MQGFLDL+++ W
Sbjct: 305 NCEDLDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPW 364

Query: 381 ARNLMTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGP 440
            RNL+TR +AI PSLIV++IGG  GAG+LIIIASMILSFELPFAL+PLLKF+S  TKMG 
Sbjct: 365 LRNLLTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSCKTKMGS 424

Query: 441 HKNSIYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRIANVFIGIVVFPLMAIYVL 487
           H N + I  ++W++G  I+GINIYYL ++F+  LIH+++  I  VF GI+ F  +A+Y+ 
Sbjct: 425 HVNPMAITALTWVIGGLIMGINIYYLVSSFIKLLIHSHMKLILVVFCGILGFAGIALYLA 484

BLAST of Lsi08G004550 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 510.8 bits (1314), Expect = 1.3e-144
Identity = 278/487 (57.08%), Postives = 354/487 (72.69%), Query Frame = 0

Query: 25  IAAVNTTERTTTPTNAVKPQNHQEEQTTTHQKPGWKKFLSHVGPGFLVSLAYLDPGNLET 84
           +AA   +    + +N+   +N    Q    +K  WK F S++GPGFLVS+AY+DPGN ET
Sbjct: 1   MAAETASGSNRSISNSPLIENSDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFET 60

Query: 85  DLQAGANHGFELLWVVLIGLIFALIIQSLAANLGVST----------------------- 144
           DLQ+GA + +ELLW++L+    AL+IQSLAANLGV T                       
Sbjct: 61  DLQSGAQYKYELLWIILVASCAALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVV 120

Query: 145 ------------VIGTAFALNILFHIPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAIL 204
                       VIGTAFALN+LF+IPVW GVLLTGLSTL+LL LQ+YG RKLE LIA L
Sbjct: 121 AEIAVVACDIPEVIGTAFALNMLFNIPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFL 180

Query: 205 VFIMAACFFGEMSYVKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALV 264
           VF +A CFF E+ Y KP    VL G+FVP+L G GAT  AI+LLGA+VMPHNLFLHSALV
Sbjct: 181 VFTIALCFFVELHYSKPDPKEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALV 240

Query: 265 LSRKVPNSVRGINDACRYFLIESGFALFIAFLINVAVVSVSGTVCTVGNVSETTADQCSD 324
           LSRK+P SV GI +ACRY+LIESG AL +AFLINV+V+SVSG VC   ++S      C D
Sbjct: 241 LSRKIPRSVTGIKEACRYYLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQD 300

Query: 325 ITLNSASFLLQNVLGKSSSIIYAIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNL 384
           + LN ASFLL+NV+GK SS ++AIAL ASGQSS+ITGTYAGQ++MQGFLDL+++ W RN 
Sbjct: 301 LDLNKASFLLRNVVGKWSSKLFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNF 360

Query: 385 MTRSIAITPSLIVSIIGGPQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNS 444
           +TR +AI PSLIV++IGG  GAG+LIIIASMILSFELPFAL+PLLKF+SS TKMG H NS
Sbjct: 361 LTRCLAIIPSLIVALIGGSAGAGKLIIIASMILSFELPFALVPLLKFTSSKTKMGSHANS 420

Query: 445 IYIIVISWILGLAIIGINIYYLSTAFVGWLIHNNLHRIANVFIGIVVFPLMAIYVLAVIY 477
           + I  ++WI+G  I+GINIYYL ++F+  L+H++++ +A VF+G++ F  +A Y+ A+ Y
Sbjct: 421 LVISSVTWIIGGLIMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISY 480

BLAST of Lsi08G004550 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 250.4 bits (638), Expect = 3.2e-66
Identity = 185/499 (37.07%), Postives = 263/499 (52.71%), Query Frame = 0

Query: 47  QEEQTTTHQKPG------WKKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVV 106
           +EE       PG      WKK     GPGFL+S+A+LDPGNLE+DLQAGA  G+ L+W++
Sbjct: 31  EEEDADYDDDPGNSPKFSWKKLWLFTGPGFLMSIAFLDPGNLESDLQAGAIAGYSLIWLL 90

Query: 107 LIGLIFALIIQSLAANLGVST-----------------------------------VIGT 166
           +      L+IQ L+A LGV+T                                   VIG+
Sbjct: 91  MWATAIGLLIQLLSARLGVATGRHLAELCREEYPTWARMVLWIMAEIALIGADIQEVIGS 150

Query: 167 AFALNILFH--IPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSY 226
           A A+ IL +  +P+WAGV++T L   + L L+ YG RKLE + AIL+  MA  F      
Sbjct: 151 AIAIKILSNGLVPLWAGVVITALDCFIFLFLENYGIRKLEAVFAILIATMALAFAWMFGQ 210

Query: 227 VKPPATGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKV-PNSVRGIN 286
            KP  T +L G  VPKL+ +     A+ ++G ++MPHN+FLHSALV SR+V P     + 
Sbjct: 211 TKPSGTELLVGALVPKLSSR-TIKQAVGIVGCIIMPHNVFLHSALVQSREVDPKKRFRVK 270

Query: 287 DACRYFLIESGFALFIAFLINVAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNV 346
           +A +Y+ IES  AL ++F+INV V +V         +++T          N+  +L    
Sbjct: 271 EALKYYSIESTGALAVSFIINVFVTTVFAKSFYGTEIADTIG------LANAGQYLQDKY 330

Query: 347 LGKSSSIIY--AIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSL 406
            G    I+Y  AI + A+GQSS+ITGTYAGQFIM GFL+LKMK W R L+TRS AI P++
Sbjct: 331 GGGFFPILYIWAIGVLAAGQSSTITGTYAGQFIMGGFLNLKMKKWVRALITRSCAIIPTM 390

Query: 407 IVSII--GGPQGAGRLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWI 466
           IV+++          L    +++ S ++PFA+IPLL   S+   MG  K    +  ISWI
Sbjct: 391 IVALVFDSSDSMLDELNEWLNVLQSVQIPFAVIPLLCLVSNEQIMGSFKIQPLVQTISWI 450

Query: 467 LGLAIIGINIYYLSTAFVGWLIHNNLHRIANVFIGIVVFPLMAIYVLAVIYLMFRKDRVV 488
           +   +I IN Y +   F G           N+ + + V      YV+ V+YL+    R +
Sbjct: 451 VAALVIAINGYLMVDFFSG--------AATNLILLVPVIIFAIAYVVFVLYLI---SRGL 510

BLAST of Lsi08G004550 vs. TAIR 10
Match: AT2G23150.1 (natural resistance-associated macrophage protein 3 )

HSP 1 Score: 238.8 bits (608), Expect = 9.8e-63
Identity = 176/457 (38.51%), Postives = 249/457 (54.49%), Query Frame = 0

Query: 59  WKKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLIFALIIQSLAANLG 118
           WKK     GPGFL+S+A+LDPGNLE DLQAGA  G+ LLW+++      L++Q L+A LG
Sbjct: 53  WKKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAVAGYSLLWLLMWATAMGLLVQLLSARLG 112

Query: 119 VST-----------------------------------VIGTAFALNILFH--IPVWAGV 178
           V+T                                   VIG+A A+ IL +  +P+WAGV
Sbjct: 113 VATGRHLAELCRDEYPTWARMVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAGV 172

Query: 179 LLTGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPATGVLKGMFVPKLN 238
           ++T L   + L L+ YG RKLE + A+L+  M   F       KP  + +L G+ VPKL+
Sbjct: 173 VITALDCFVFLFLENYGIRKLEAVFAVLIATMGVSFAWMFGQAKPSGSELLIGILVPKLS 232

Query: 239 GQGATADAIALLGALVMPHNLFLHSALVLSRKVPNSVR-GINDACRYFLIESGFALFIAF 298
            +     A+ ++G ++MPHN+FLHSALV SR+V    +  + +A  Y+ IES  ALFI+F
Sbjct: 233 SR-TIQKAVGVVGCIIMPHNVFLHSALVQSREVDKRQKYRVQEALNYYTIESTIALFISF 292

Query: 299 LINVAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSSSIIY--AIALFAS 358
           LIN+ V     TV   G  +   A+      +N+  +L +   G    I+Y  AI L A+
Sbjct: 293 LINLFVT----TVFAKGFYNTDLANSIG--LVNAGQYLQEKYGGGVFPILYIWAIGLLAA 352

Query: 359 GQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSIIGGPQGAGRLII-- 418
           GQSS+ITGTYAGQFIM GFL+ KMK W R L+TRS AI P++IV+++     A   ++  
Sbjct: 353 GQSSTITGTYAGQFIMGGFLNFKMKKWLRALITRSCAIIPTIIVALVFDSSEATLDVLNE 412

Query: 419 IASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAIIGINIYYLSTAFV 474
             +++ S ++PFALIPLL   S    MG  K       I+W++   +I IN Y L   F 
Sbjct: 413 WLNVLQSIQIPFALIPLLCLVSKEQIMGSFKIGPLYKTIAWLVAALVIMINGYLLLEFF- 472

BLAST of Lsi08G004550 vs. TAIR 10
Match: AT1G47240.1 (NRAMP metal ion transporter 2 )

HSP 1 Score: 235.3 bits (599), Expect = 1.1e-61
Identity = 168/468 (35.90%), Postives = 248/468 (52.99%), Query Frame = 0

Query: 48  EEQTTTHQKP--GWKKFLSHVGPGFLVSLAYLDPGNLETDLQAGANHGFELLWVVLIGLI 107
           ++ TT    P   W+K     GPGFL+S+A+LDPGNLE DLQAGA  G+ LLW+++    
Sbjct: 52  DDPTTGDTPPPFSWRKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATA 111

Query: 108 FALIIQSLAANLGVST-----------------------------------VIGTAFALN 167
             L+IQ L+A +GV+T                                   VIG+A A+ 
Sbjct: 112 MGLLIQMLSARVGVATGRHLAELCRDEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQ 171

Query: 168 ILFH--IPVWAGVLLTGLSTLLLLGLQRYGARKLELLIAILVFIMAACFFGEMSYVKPPA 227
           IL    +P+WAGV++T     L L L+ YG RKLE + A+L+  M   F       KP  
Sbjct: 172 ILSRGFLPLWAGVVITASDCFLFLFLENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSG 231

Query: 228 TGVLKGMFVPKLNGQGATADAIALLGALVMPHNLFLHSALVLSRKV-PNSVRGINDACRY 287
             ++ G+ +P+L+ +     A+ ++G ++MPHN+FLHSALV SRK+ P     + +A  Y
Sbjct: 232 KELMIGILLPRLSSK-TIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNY 291

Query: 288 FLIESGFALFIAFLINVAVVSVSGTVCTVGNVSETTADQCSDITLNSASFLLQNVLGKSS 347
           +LIES  ALFI+F+IN+ V     TV   G      A+      +N+  +L +   G   
Sbjct: 292 YLIESSVALFISFMINLFVT----TVFAKGFYGTEKANNIG--LVNAGQYLQEKFGGGLL 351

Query: 348 SIIY--AIALFASGQSSSITGTYAGQFIMQGFLDLKMKTWARNLMTRSIAITPSLIVSII 407
            I+Y   I L A+GQSS+ITGTYAGQFIM GFL+L++K W R ++TRS AI P++IV+I+
Sbjct: 352 PILYIWGIGLLAAGQSSTITGTYAGQFIMGGFLNLRLKKWMRAVITRSCAIVPTMIVAIV 411

Query: 408 GGPQGAGRLII--IASMILSFELPFALIPLLKFSSSSTKMGPHKNSIYIIVISWILGLAI 467
                A   ++    +++ S ++PFAL+PLL   S    MG  K    +  I+W +   +
Sbjct: 412 FNTSEASLDVLNEWLNVLQSVQIPFALLPLLTLVSKEEIMGDFKIGPILQRIAWTVAALV 471

Query: 468 IGINIYYLSTAFVGWLIHNNLHRIANVFIGIVVFPLMAIYVLAVIYLM 472
           + IN Y L   FV          +     G+ V      Y+  ++YL+
Sbjct: 472 MIINGYLLLDFFVS--------EVDGFLFGVTVCVWTTAYIAFIVYLI 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H4H54.4e-18567.99Metal transporter Nramp5 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP5 PE=2... [more]
Q0D7E44.0e-17066.25Metal transporter Nramp1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP1 PE=2... [more]
Q6ZG858.4e-15257.68Metal transporter NRAT1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAT1 PE=2 S... [more]
Q9SAH82.2e-14456.29Metal transporter Nramp1 OS=Arabidopsis thaliana OX=3702 GN=NRAMP1 PE=1 SV=1[more]
Q9S9N81.9e-14357.08Metal transporter Nramp6 OS=Arabidopsis thaliana OX=3702 GN=NRAMP6 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KDB86.3e-25988.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G382880 PE=3 SV=1[more]
A0A6J1GNU91.0e-25686.33metal transporter Nramp5-like OS=Cucurbita moschata OX=3662 GN=LOC111455783 PE=3... [more]
A0A1S3CA246.5e-25687.16metal transporter Nramp5-like OS=Cucumis melo OX=3656 GN=LOC103498358 PE=3 SV=1[more]
A0A6J1JPX47.2e-25585.79metal transporter Nramp5-like OS=Cucurbita maxima OX=3661 GN=LOC111487830 PE=3 S... [more]
A0A6J1CJ541.6e-24182.68metal transporter Nramp5-like OS=Momordica charantia OX=3673 GN=LOC111011923 PE=... [more]
Match NameE-valueIdentityDescription
XP_038886296.14.7e-26990.32metal transporter Nramp5-like [Benincasa hispida][more]
KAG7032815.19.6e-26292.13Metal transporter Nramp5, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_011658448.21.3e-25888.07metal transporter Nramp5 [Cucumis sativus] >KGN47700.1 hypothetical protein Csa_... [more]
XP_023526032.13.2e-25786.33metal transporter Nramp5-like [Cucurbita pepo subsp. pepo][more]
XP_022953164.12.1e-25686.33metal transporter Nramp5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G80830.11.6e-14556.29natural resistance-associated macrophage protein 1 [more]
AT1G15960.11.3e-14457.08NRAMP metal ion transporter 6 [more]
AT5G67330.13.2e-6637.07natural resistance associated macrophage protein 4 [more]
AT2G23150.19.8e-6338.51natural resistance-associated macrophage protein 3 [more]
AT1G47240.11.1e-6135.90NRAMP metal ion transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001046NRAMP familyPRINTSPR00447NATRESASSCMPcoord: 134..153
score: 44.0
coord: 379..398
score: 49.2
coord: 159..180
score: 43.64
coord: 209..232
score: 63.0
coord: 319..338
score: 73.0
coord: 106..132
score: 31.85
coord: 347..364
score: 53.78
IPR001046NRAMP familyPFAMPF01566Nrampcoord: 120..409
e-value: 4.0E-85
score: 285.9
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 122..523
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 52..121
IPR001046NRAMP familyHAMAPMF_00221NRAMPcoord: 52..435
score: 33.958679
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..57
NoneNo IPR availablePANTHERPTHR11706:SF77METAL TRANSPORTER NRAMP5coord: 52..121
NoneNo IPR availablePANTHERPTHR11706:SF77METAL TRANSPORTER NRAMP5coord: 122..523

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G004550.1Lsi08G004550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070574 cadmium ion transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:0030001 metal ion transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015086 cadmium ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity