Lsi07G011840 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G011840
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein TORNADO 2-like
Locationchr07: 17344260 .. 17345863 (+)
RNA-Seq ExpressionLsi07G011840
SyntenyLsi07G011840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAAACCCTAAAAAATTTCCTTCAAAACAAGGCAAATAAGACCCCACACAAAACTTCCCTTCACAAAGCTTCACAAAAAACAACCTCTTAACAATGGCATTGAGCAACAATGTCATTGCAGCCATAAACTTCATTGCCTTGCTTCTCTCGATCCCCGTCATCGGTGCCGGGATATGGCTCGCAACTGAAGCAGACAACGCCTGCGTGCAGATTTTGCAATGGCCGGTTATCGTTCTCGGCATCGTCATCTTTCTAGTGGCTTTGGCAGGTTTCATTGGAGCATTTTGGAGAATCTCATGGCTTCTAATGTTCTACCTCATTGCCATGCTTGTTCTCATAATTCTACTCGGTAGCTTGGTTATCTTTGTCTTCTTCGTCACGATGAGAGGATCGGGGCACACCGAACCGAGCCGAGCTTACTTGGAATATCATCTTGATGATTTCTCCCTTTGGCTCCAAAGAAGGGTCAGAAGCTCTCATAAATGGGAAAGAATTAGAAATTGCCTTAGTTCTACTACTACATGTGCTGAGTTGAACCAAAACTATAGATTAGCTCAAGATTTCTTCAATGCCCACATCACCCCTTTCCAGGTAAAATTCTTTACTTTATATATATCATCCCAAAACTTTACTTTAGGAGGGAAAATTAGAGAAAGTTTTGTTTTTTATTTTTTTTAATTTTGAAATTTGAAAATTAAGTTTCTAAAATGCTTACTTTCACCTCTAAATTTCTATGTTATATTGTCCACTTTCTACCAATATTTTCAAAAACAAAGTCAAATTTTGAAAATGAAAAAAAGTAGGCTTTTTTTAGAAGAAATTGAAAAAAAGTAATTTTTGTTTTTAGATGTTGATTAAGAATTTAAATGTTTCTTTAATAAACATAATTGGGAGCAAACAAACATAGATTTTAAGAAAATTTGAAAACTATAAGAAAATTTGAAAACTAAAAACGAAAGAGTTACCAAAATGAGCCTTTTAACTTTTTATATTAAATTTTAAATCTATAACAAAAATTAGGTGCAATCAAAGAGTTACCAAAGTATTTCGAAATGATTTAGAATATGAAAAAAACGATTTTAACAATTTCAAAATCACTCCCAAAATATACATTTAGACTCTCGATTTTCTAAGAGCTTAATTTTTTTTTTTACTTTATGAAGTACTTAATTAAAAAATCATTCCAAACAGATTCTAAGTCGATAGAGATTCTAATATATATTTAATCTTTTATACATATTCTTTCACCAGTCTGGGTGTTGTAAGCCCCCAACAGATTGTGGTTTCACGTTCATAAACCCGACGTACTGGATTAGTCCGATTAGCAATGGAGCGAGCACGGATTGTTCGAAATGGAGCAACGAGCAAACGGAGCTTTGCTATGGATGTGATTCATGTAAAGCAGGGCTACTAGCCAACCTGAAGGAAGAATGGAGAAATGCAGACTTAATTCTATTGATTGCACTTTTGGGTCTTGTTTTTGTGTATTTGATGGCTTTTTTTGCTTTGAAAAATGCCAAATCTGAGGACCTTTTCCGCAGGCACAAGCAAGGCTATACCTAACACTCTCAAATATCAACGAAAACATAACCTTTAATTTTAT

mRNA sequence

TGAAAACCCTAAAAAATTTCCTTCAAAACAAGGCAAATAAGACCCCACACAAAACTTCCCTTCACAAAGCTTCACAAAAAACAACCTCTTAACAATGGCATTGAGCAACAATGTCATTGCAGCCATAAACTTCATTGCCTTGCTTCTCTCGATCCCCGTCATCGGTGCCGGGATATGGCTCGCAACTGAAGCAGACAACGCCTGCGTGCAGATTTTGCAATGGCCGGTTATCGTTCTCGGCATCGTCATCTTTCTAGTGGCTTTGGCAGGTTTCATTGGAGCATTTTGGAGAATCTCATGGCTTCTAATGTTCTACCTCATTGCCATGCTTGTTCTCATAATTCTACTCGGTAGCTTGGTTATCTTTGTCTTCTTCGTCACGATGAGAGGATCGGGGCACACCGAACCGAGCCGAGCTTACTTGGAATATCATCTTGATGATTTCTCCCTTTGGCTCCAAAGAAGGGTCAGAAGCTCTCATAAATGGGAAAGAATTAGAAATTGCCTTAGTTCTACTACTACATGTGCTGAGTTGAACCAAAACTATAGATTAGCTCAAGATTTCTTCAATGCCCACATCACCCCTTTCCAGTCTGGGTGTTGTAAGCCCCCAACAGATTGTGGTTTCACGTTCATAAACCCGACGTACTGGATTAGTCCGATTAGCAATGGAGCGAGCACGGATTGTTCGAAATGGAGCAACGAGCAAACGGAGCTTTGCTATGGATGTGATTCATGTAAAGCAGGGCTACTAGCCAACCTGAAGGAAGAATGGAGAAATGCAGACTTAATTCTATTGATTGCACTTTTGGGTCTTGTTTTTGTGTATTTGATGGCTTTTTTTGCTTTGAAAAATGCCAAATCTGAGGACCTTTTCCGCAGGCACAAGCAAGGCTATACCTAACACTCTCAAATATCAACGAAAACATAACCTTTAATTTTAT

Coding sequence (CDS)

ATGGCATTGAGCAACAATGTCATTGCAGCCATAAACTTCATTGCCTTGCTTCTCTCGATCCCCGTCATCGGTGCCGGGATATGGCTCGCAACTGAAGCAGACAACGCCTGCGTGCAGATTTTGCAATGGCCGGTTATCGTTCTCGGCATCGTCATCTTTCTAGTGGCTTTGGCAGGTTTCATTGGAGCATTTTGGAGAATCTCATGGCTTCTAATGTTCTACCTCATTGCCATGCTTGTTCTCATAATTCTACTCGGTAGCTTGGTTATCTTTGTCTTCTTCGTCACGATGAGAGGATCGGGGCACACCGAACCGAGCCGAGCTTACTTGGAATATCATCTTGATGATTTCTCCCTTTGGCTCCAAAGAAGGGTCAGAAGCTCTCATAAATGGGAAAGAATTAGAAATTGCCTTAGTTCTACTACTACATGTGCTGAGTTGAACCAAAACTATAGATTAGCTCAAGATTTCTTCAATGCCCACATCACCCCTTTCCAGTCTGGGTGTTGTAAGCCCCCAACAGATTGTGGTTTCACGTTCATAAACCCGACGTACTGGATTAGTCCGATTAGCAATGGAGCGAGCACGGATTGTTCGAAATGGAGCAACGAGCAAACGGAGCTTTGCTATGGATGTGATTCATGTAAAGCAGGGCTACTAGCCAACCTGAAGGAAGAATGGAGAAATGCAGACTTAATTCTATTGATTGCACTTTTGGGTCTTGTTTTTGTGTATTTGATGGCTTTTTTTGCTTTGAAAAATGCCAAATCTGAGGACCTTTTCCGCAGGCACAAGCAAGGCTATACCTAA

Protein sequence

MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGFIGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLWLQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTFINPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLGLVFVYLMAFFALKNAKSEDLFRRHKQGYT
Homology
BLAST of Lsi07G011840 vs. ExPASy Swiss-Prot
Match: Q9FIQ5 (Protein TORNADO 2 OS=Arabidopsis thaliana OX=3702 GN=TRN2 PE=1 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 5.0e-108
Identity = 187/269 (69.52%), Postives = 222/269 (82.53%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           M LSNNVI  INFI +LLSIPVIGAGIWLA    N+CV++LQWPVI+LG++I LV LAGF
Sbjct: 1   MPLSNNVIGCINFITVLLSIPVIGAGIWLAIGTVNSCVKLLQWPVIILGVLILLVGLAGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IG FWRI+WLL+ YLIAML+LI+LLG LV F++ VT+RGSGH EPSRAYLEY L DFS W
Sbjct: 61  IGGFWRITWLLVVYLIAMLILIVLLGCLVGFIYMVTIRGSGHPEPSRAYLEYSLQDFSGW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           L+RRV+ S+KWERIR CLS+TT C ELNQ Y LAQDFFNAH+ P QSGCCKPPT CGFTF
Sbjct: 121 LRRRVQRSYKWERIRTCLSTTTICPELNQRYTLAQDFFNAHLDPIQSGCCKPPTKCGFTF 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPI   A  DC  WSN+Q  LCY CDSCKAGLLAN+K +W  AD+ LL+AL+G
Sbjct: 181 VNPTYWISPIDMSADMDCLNWSNDQNTLCYTCDSCKAGLLANIKVDWLKADIFLLLALIG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGYT 270
           L+ VY++   A +NA++ED+FR++KQGYT
Sbjct: 241 LIIVYIIGCCAFRNAETEDIFRKYKQGYT 269

BLAST of Lsi07G011840 vs. ExPASy Swiss-Prot
Match: Q9ZUN5 (Tetraspanin-2 OS=Arabidopsis thaliana OX=3702 GN=TET2 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 9.5e-75
Identity = 138/269 (51.30%), Postives = 191/269 (71.00%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MAL+NN+ A +N +ALL SIP+  +GIWLA++ DN CV +L+WPV+VLG++I +V+  GF
Sbjct: 1   MALANNLTAILNLLALLCSIPITASGIWLASKPDNECVNLLRWPVVVLGVLILVVSATGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGA+     LL  YL  M +LI LL  ++IF F VT     +  P R Y EY L+ FS W
Sbjct: 61  IGAYKYKETLLAVYLCCMAILIGLLLVVLIFAFVVTRPDGSYRVPGRGYKEYRLEGFSNW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFF-NAHITPFQSGCCKPPTDCGFT 180
           L+  V  S  W R+R CL+ T  C +LNQ +  A  FF ++ ITP QSGCCKPPT CG+ 
Sbjct: 121 LKENVVDSKNWGRLRACLADTNVCPKLNQEFITADQFFSSSKITPLQSGCCKPPTACGYN 180

Query: 181 FINPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALL 240
           F+NPT W++P +  A  DC  WSN+Q++LCY C+SCKAGLL NL++EWR A+LIL+I ++
Sbjct: 181 FVNPTLWLNPTNMAADADCYLWSNDQSQLCYNCNSCKAGLLGNLRKEWRKANLILIITVV 240

Query: 241 GLVFVYLMAFFALKNAKSEDLFRRHKQGY 269
            L++VY++A  A +NA++EDLFR++KQG+
Sbjct: 241 VLIWVYVIACSAFRNAQTEDLFRKYKQGW 269

BLAST of Lsi07G011840 vs. ExPASy Swiss-Prot
Match: Q9M1E7 (Tetraspanin-3 OS=Arabidopsis thaliana OX=3702 GN=TET3 PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.5e-59
Identity = 117/265 (44.15%), Postives = 176/265 (66.42%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNA-CVQILQWPVIVLGIVIFLVALAG 60
           M  SN++I  +NF+  LLSIP++G GIWL++ A++  C++ LQWP+IV+GI I +V+LAG
Sbjct: 1   MRTSNHLIGLVNFLTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIVIGISIMVVSLAG 60

Query: 61  FIGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSL 120
           F GA +R  +L+  YL+ ML++I  L   +IF + VT +GSG T  +R YL+Y+L+D+S 
Sbjct: 61  FAGACYRNKFLMWLYLVVMLLIIAALIGFIIFAYAVTDKGSGRTVLNRGYLDYYLEDYSG 120

Query: 121 WLQRRVRSSHKWERIRNCLSSTTTCAELNQNY----RLAQDFFNAHITPFQSGCCKPPTD 180
           WL+ RV     W +I +CL  +  C ++ +N+      A  FF   ++P +SGCCKPPTD
Sbjct: 121 WLKDRVSDDSYWGKISSCLRDSGACRKIGRNFNGVPETADMFFLRRLSPVESGCCKPPTD 180

Query: 181 CGFTFINPTYWISPISN-GASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLIL 240
           CGF+++N T W +     G + DC  WSN+Q+ LCY C SCKAG+L +LK+ WR   +I 
Sbjct: 181 CGFSYVNETGWDTRGGMIGPNQDCMVWSNDQSMLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

Query: 241 LIALLGLVFVYLMAFFALKNAKSED 260
           ++ L+ LV  Y++A+ A +N K  D
Sbjct: 241 IVVLIILVIFYVIAYAAYRNVKRID 265

BLAST of Lsi07G011840 vs. ExPASy Swiss-Prot
Match: Q8S8Q6 (Tetraspanin-8 OS=Arabidopsis thaliana OX=3702 GN=TET8 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 9.9e-56
Identity = 104/263 (39.54%), Postives = 159/263 (60.46%), Query Frame = 0

Query: 4   SNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGFIGA 63
           SNN++  +NF+  LLSIP++  GIWL+ +    C + L  PVI LG+ + +VA+AG IG+
Sbjct: 5   SNNLVGILNFLVFLLSIPILAGGIWLSQKGSTECERFLDKPVIALGVFLMVVAIAGLIGS 64

Query: 64  FWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLWLQR 123
             R++WLL  YL  M +LI+L+  + +F F VT +G+G     + Y EY L D+S WLQ+
Sbjct: 65  CCRVTWLLWVYLFVMFLLILLVFCITVFAFVVTNKGAGEAIEGKGYKEYKLGDYSTWLQK 124

Query: 124 RVRSSHKWERIRNCLSSTTTCAELNQNY--RLAQDFFNAHITPFQSGCCKPPTDCGFTFI 183
           RV +   W +IR+CL  +  C++L   +       F+  H+T  QSGCCKP  +CGF ++
Sbjct: 125 RVENGKNWNKIRSCLVESKVCSKLEAKFVNVPVNSFYKEHLTALQSGCCKPSDECGFEYV 184

Query: 184 NPTYWI-SPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 243
           NPT W  +      + DC  W N + +LC+ C SCKAGLL N+K  W+   ++ ++ L+ 
Sbjct: 185 NPTTWTKNTTGTHTNPDCQTWDNAKEKLCFDCQSCKAGLLDNVKSAWKKVAIVNIVFLVF 244

Query: 244 LVFVYLMAFFALKNAKSEDLFRR 264
           L+ VY +   A +N K +D + R
Sbjct: 245 LIIVYSVGCCAFRNNKRDDSYSR 267

BLAST of Lsi07G011840 vs. ExPASy Swiss-Prot
Match: Q9SUD4 (Tetraspanin-7 OS=Arabidopsis thaliana OX=3702 GN=TET7 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.9e-55
Identity = 108/253 (42.69%), Postives = 153/253 (60.47%), Query Frame = 0

Query: 4   SNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGFIGA 63
           SNN++  +NF   LLSIP++ AGIWL   A   C + L  P++VLGI +  V++AG +GA
Sbjct: 5   SNNLLGILNFFTFLLSIPILSAGIWLGKNAATECERFLDKPMVVLGIFLMFVSIAGLVGA 64

Query: 64  FWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLWLQR 123
             R+S LL  YL AM +LI+L     IF F VT RG+G     R Y EYH+ D+S WLQ+
Sbjct: 65  CCRVSCLLWLYLFAMFLLILLGFCFTIFAFAVTNRGAGEVISDRGYKEYHVADYSNWLQK 124

Query: 124 RVRSSHKWERIRNCLSSTTTCAELNQNYRL--AQDFFNAHITPFQSGCCKPPTDCGFTFI 183
           RV ++  WERIR+CL  +  C+     Y     +DF+ +++   QSGCCKP  DC FT++
Sbjct: 125 RVNNAKNWERIRSCLMYSDVCSTYRTRYASINVEDFYKSNLNALQSGCCKPSNDCNFTYV 184

Query: 184 NPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLGL 243
           NPT W        + DC+ W N+   LCY C++CKAGLL N+K  W+    + ++ L+ L
Sbjct: 185 NPTTWTKTPGPYKNEDCNVWDNKPGTLCYDCEACKAGLLDNIKNSWKKVAKVNIVFLIFL 244

Query: 244 VFVYLMAFFALKN 255
           + VY +   A +N
Sbjct: 245 IIVYSVGCCAFRN 257

BLAST of Lsi07G011840 vs. ExPASy TrEMBL
Match: A0A6J1EBD8 (protein TORNADO 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431037 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 6.7e-140
Identity = 250/268 (93.28%), Postives = 260/268 (97.01%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIALLLSIP+IGAGIWLAT+ADNACVQ+LQWPVI+LGIVI LVAL GF
Sbjct: 1   MALSNNVIAAINFIALLLSIPIIGAGIWLATQADNACVQLLQWPVIILGIVILLVALTGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWRISWLLMFYLIAMLVLIILLGSLV+FVF VTMRGSGHTEPSRAYLEYHLDDFSLW
Sbjct: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVLFVFSVTMRGSGHTEPSRAYLEYHLDDFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIR CLSSTTTCAELNQNYRL QDFFNAHI+P QSGCCKPPTDCGFT+
Sbjct: 121 LQRRVRSSHKWERIRTCLSSTTTCAELNQNYRLPQDFFNAHISPLQSGCCKPPTDCGFTY 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIAL+G
Sbjct: 181 VNPTYWISPISNGASVDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALIG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGY 269
           LVFVYLMAF ALKNAKSE+LFRRHKQGY
Sbjct: 241 LVFVYLMAFLALKNAKSENLFRRHKQGY 268

BLAST of Lsi07G011840 vs. ExPASy TrEMBL
Match: A0A1S3BL48 (protein TORNADO 2-like OS=Cucumis melo OX=3656 GN=LOC103490773 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.1e-139
Identity = 247/268 (92.16%), Postives = 259/268 (96.64%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIALLLSIPVIGAGIWLATE DNACVQ+LQWPVI+LGIVIFLVALAGF
Sbjct: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEPDNACVQVLQWPVIILGIVIFLVALAGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWR+SWLLMFYL+AMLVLIILLGSLVIFVF VTMRGSGH EPSRAYLEYHLD+FSLW
Sbjct: 61  IGAFWRVSWLLMFYLVAMLVLIILLGSLVIFVFSVTMRGSGHAEPSRAYLEYHLDEFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIRNCLSS+TTCAELNQNYR AQDFFNAHITPFQSGCCKPPTDCGFTF
Sbjct: 121 LQRRVRSSHKWERIRNCLSSSTTCAELNQNYRFAQDFFNAHITPFQSGCCKPPTDCGFTF 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLANL+EEWRNAD+ILL++ LG
Sbjct: 181 VNPTYWISPISNGASMDCSKWSNEQTELCYGCDSCKAGLLANLREEWRNADIILLVSFLG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGY 269
           L+FVYLMAF ALKNA SE LFRRHKQGY
Sbjct: 241 LIFVYLMAFIALKNANSEQLFRRHKQGY 268

BLAST of Lsi07G011840 vs. ExPASy TrEMBL
Match: A0A0A0L3X4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G009500 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.4e-139
Identity = 245/268 (91.42%), Postives = 259/268 (96.64%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIAL+LSIPVIGAGIWLATE DNACVQ+LQWPVI+LGIVIFLVALAGF
Sbjct: 1   MALSNNVIAAINFIALILSIPVIGAGIWLATEPDNACVQVLQWPVIILGIVIFLVALAGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWR+SWLLMFYLIAMLVLIILLGSL+IFVF VTMRGSGH EPSRAYLEYHLD+FSLW
Sbjct: 61  IGAFWRVSWLLMFYLIAMLVLIILLGSLIIFVFSVTMRGSGHAEPSRAYLEYHLDEFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIRNCLSS+TTCAELNQNYR AQDFFNAHITPFQSGCCKPPTDCGFTF
Sbjct: 121 LQRRVRSSHKWERIRNCLSSSTTCAELNQNYRFAQDFFNAHITPFQSGCCKPPTDCGFTF 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNAD+ILL++ +G
Sbjct: 181 VNPTYWISPISNGASMDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADIILLVSFVG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGY 269
           L+FVYL+AF ALKNA SE LFRRHKQGY
Sbjct: 241 LIFVYLLAFIALKNANSEQLFRRHKQGY 268

BLAST of Lsi07G011840 vs. ExPASy TrEMBL
Match: A0A6J1HK71 (protein TORNADO 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465262 PE=4 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 5.7e-139
Identity = 250/268 (93.28%), Postives = 260/268 (97.01%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIALLLSIP+IGAGIW+AT+ADNACVQ+LQWPVIVLGIVIFLVAL GF
Sbjct: 1   MALSNNVIAAINFIALLLSIPIIGAGIWIATQADNACVQLLQWPVIVLGIVIFLVALTGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWRIS LLMFYLIAMLVLIILLGSLV+FVF VTMRGSGHTEPSRAYLEYHLDDFSLW
Sbjct: 61  IGAFWRISGLLMFYLIAMLVLIILLGSLVLFVFSVTMRGSGHTEPSRAYLEYHLDDFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIR CLSSTTTCAELNQNYRL QDFFNAHI+P QSGCCKPPTDCGFT+
Sbjct: 121 LQRRVRSSHKWERIRTCLSSTTTCAELNQNYRLPQDFFNAHISPLQSGCCKPPTDCGFTY 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIAL+G
Sbjct: 181 VNPTYWISPISNGASVDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALIG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGY 269
           LVFVYLMAF ALKNAKSE+LFRRHKQGY
Sbjct: 241 LVFVYLMAFLALKNAKSENLFRRHKQGY 268

BLAST of Lsi07G011840 vs. ExPASy TrEMBL
Match: A0A5N6R678 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_012453 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.5e-118
Identity = 204/269 (75.84%), Postives = 243/269 (90.33%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVI AINF+A+LLS+P+IG+GIWLATE DN+CV+ILQWPVI+LG++I +VALAGF
Sbjct: 1   MALSNNVIGAINFVAMLLSVPIIGSGIWLATEPDNSCVKILQWPVIILGVLILVVALAGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           +GAFWRI WLL+FYL+AMLVLIILL  LV+F++ VTMRGSGH EPSRAYLEYHLDD+S W
Sbjct: 61  VGAFWRIPWLLIFYLVAMLVLIILLACLVVFIYMVTMRGSGHLEPSRAYLEYHLDDYSGW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           L+RRV+SS+KWERIRNCLSSTT CAEL+Q+YR+AQDFFNAHITP QSGCCKPPT CG+TF
Sbjct: 121 LRRRVQSSYKWERIRNCLSSTTMCAELSQSYRMAQDFFNAHITPLQSGCCKPPTQCGYTF 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPI+  A  DC +W+NEQT+LCY CDSCKAGLLANLK+EWR AD+ILLI L+ 
Sbjct: 181 VNPTYWISPINTAADMDCLQWNNEQTQLCYNCDSCKAGLLANLKKEWRRADIILLITLVA 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGYT 270
           L+ VYL+   A +NAK+EDLFR++KQGYT
Sbjct: 241 LISVYLIGCCAFRNAKTEDLFRKYKQGYT 269

BLAST of Lsi07G011840 vs. NCBI nr
Match: XP_038905565.1 (protein TORNADO 2 [Benincasa hispida])

HSP 1 Score: 511.9 bits (1317), Expect = 3.3e-141
Identity = 251/269 (93.31%), Postives = 264/269 (98.14%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQ+LQWPVI+LGIVIFLVALAGF
Sbjct: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQVLQWPVIILGIVIFLVALAGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWRISWLLMFYL+AMLVLIILLGSLVIFVF VTMRGSGH EPSRAYLEYHLD+FSLW
Sbjct: 61  IGAFWRISWLLMFYLVAMLVLIILLGSLVIFVFSVTMRGSGHAEPSRAYLEYHLDEFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIR+CLSS+TTCAELNQNYR AQDFFNAHITPFQSGCCKPPTDCGFTF
Sbjct: 121 LQRRVRSSHKWERIRSCLSSSTTCAELNQNYRFAQDFFNAHITPFQSGCCKPPTDCGFTF 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLAN+KEEWRNA++ILLIAL+G
Sbjct: 181 VNPTYWISPISNGASMDCSKWSNEQTELCYGCDSCKAGLLANIKEEWRNANIILLIALVG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGYT 270
           L+FVYLMAFFAL+NAKSEDLFRRH QGYT
Sbjct: 241 LIFVYLMAFFALRNAKSEDLFRRHTQGYT 269

BLAST of Lsi07G011840 vs. NCBI nr
Match: XP_023552937.1 (protein TORNADO 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 506.9 bits (1304), Expect = 1.1e-139
Identity = 251/268 (93.66%), Postives = 260/268 (97.01%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIALLLSIP+IGAGIWLAT+ADNACVQ+LQWPVIVLGIVI LVAL GF
Sbjct: 1   MALSNNVIAAINFIALLLSIPIIGAGIWLATQADNACVQVLQWPVIVLGIVILLVALTGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWRISWLLMFYLIAMLVLIILLGSLV+FVF VTMRGSGHTEPSRAYLEYHLDDFSLW
Sbjct: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVLFVFSVTMRGSGHTEPSRAYLEYHLDDFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIR CLSSTTTCAELNQNYRL QDFFNAHI+P QSGCCKPPTDCGFT+
Sbjct: 121 LQRRVRSSHKWERIRTCLSSTTTCAELNQNYRLPQDFFNAHISPLQSGCCKPPTDCGFTY 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIAL+G
Sbjct: 181 VNPTYWISPISNGASMDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALIG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGY 269
           LVFVYLMAF ALKNAKSE+LFRRHKQGY
Sbjct: 241 LVFVYLMAFLALKNAKSENLFRRHKQGY 268

BLAST of Lsi07G011840 vs. NCBI nr
Match: XP_022923295.1 (protein TORNADO 2-like [Cucurbita moschata])

HSP 1 Score: 506.5 bits (1303), Expect = 1.4e-139
Identity = 250/268 (93.28%), Postives = 260/268 (97.01%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIALLLSIP+IGAGIWLAT+ADNACVQ+LQWPVI+LGIVI LVAL GF
Sbjct: 1   MALSNNVIAAINFIALLLSIPIIGAGIWLATQADNACVQLLQWPVIILGIVILLVALTGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWRISWLLMFYLIAMLVLIILLGSLV+FVF VTMRGSGHTEPSRAYLEYHLDDFSLW
Sbjct: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVLFVFSVTMRGSGHTEPSRAYLEYHLDDFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIR CLSSTTTCAELNQNYRL QDFFNAHI+P QSGCCKPPTDCGFT+
Sbjct: 121 LQRRVRSSHKWERIRTCLSSTTTCAELNQNYRLPQDFFNAHISPLQSGCCKPPTDCGFTY 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIAL+G
Sbjct: 181 VNPTYWISPISNGASVDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALIG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGY 269
           LVFVYLMAF ALKNAKSE+LFRRHKQGY
Sbjct: 241 LVFVYLMAFLALKNAKSENLFRRHKQGY 268

BLAST of Lsi07G011840 vs. NCBI nr
Match: XP_008448672.1 (PREDICTED: protein TORNADO 2-like [Cucumis melo])

HSP 1 Score: 505.8 bits (1301), Expect = 2.4e-139
Identity = 247/268 (92.16%), Postives = 259/268 (96.64%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIALLLSIPVIGAGIWLATE DNACVQ+LQWPVI+LGIVIFLVALAGF
Sbjct: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEPDNACVQVLQWPVIILGIVIFLVALAGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWR+SWLLMFYL+AMLVLIILLGSLVIFVF VTMRGSGH EPSRAYLEYHLD+FSLW
Sbjct: 61  IGAFWRVSWLLMFYLVAMLVLIILLGSLVIFVFSVTMRGSGHAEPSRAYLEYHLDEFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIRNCLSS+TTCAELNQNYR AQDFFNAHITPFQSGCCKPPTDCGFTF
Sbjct: 121 LQRRVRSSHKWERIRNCLSSSTTCAELNQNYRFAQDFFNAHITPFQSGCCKPPTDCGFTF 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLANL+EEWRNAD+ILL++ LG
Sbjct: 181 VNPTYWISPISNGASMDCSKWSNEQTELCYGCDSCKAGLLANLREEWRNADIILLVSFLG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGY 269
           L+FVYLMAF ALKNA SE LFRRHKQGY
Sbjct: 241 LIFVYLMAFIALKNANSEQLFRRHKQGY 268

BLAST of Lsi07G011840 vs. NCBI nr
Match: KAG7015753.1 (Protein TORNADO 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 505.4 bits (1300), Expect = 3.1e-139
Identity = 250/268 (93.28%), Postives = 259/268 (96.64%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MALSNNVIAAINFIALLLSIP+IGAGIWLAT+ADNACVQ+LQWPVIVLGIVI LVAL GF
Sbjct: 1   MALSNNVIAAINFIALLLSIPIIGAGIWLATQADNACVQLLQWPVIVLGIVILLVALTGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGAFWRISWLLMFYLIAM VLIILLGSLV+FVF VTMRGSGHTEPSRAYLEYHLDDFSLW
Sbjct: 61  IGAFWRISWLLMFYLIAMFVLIILLGSLVLFVFSVTMRGSGHTEPSRAYLEYHLDDFSLW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           LQRRVRSSHKWERIR CLSSTTTCAELNQNYRL QDFFNAHI+P QSGCCKPPTDCGFT+
Sbjct: 121 LQRRVRSSHKWERIRTCLSSTTTCAELNQNYRLPQDFFNAHISPLQSGCCKPPTDCGFTY 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPISNGAS DCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIAL+G
Sbjct: 181 VNPTYWISPISNGASVDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALIG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGY 269
           LVFVYLMAF ALKNAKSE+LFRRHKQGY
Sbjct: 241 LVFVYLMAFLALKNAKSENLFRRHKQGY 268

BLAST of Lsi07G011840 vs. TAIR 10
Match: AT5G46700.1 (Tetraspanin family protein )

HSP 1 Score: 392.1 bits (1006), Expect = 3.6e-109
Identity = 187/269 (69.52%), Postives = 222/269 (82.53%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           M LSNNVI  INFI +LLSIPVIGAGIWLA    N+CV++LQWPVI+LG++I LV LAGF
Sbjct: 1   MPLSNNVIGCINFITVLLSIPVIGAGIWLAIGTVNSCVKLLQWPVIILGVLILLVGLAGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IG FWRI+WLL+ YLIAML+LI+LLG LV F++ VT+RGSGH EPSRAYLEY L DFS W
Sbjct: 61  IGGFWRITWLLVVYLIAMLILIVLLGCLVGFIYMVTIRGSGHPEPSRAYLEYSLQDFSGW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFFNAHITPFQSGCCKPPTDCGFTF 180
           L+RRV+ S+KWERIR CLS+TT C ELNQ Y LAQDFFNAH+ P QSGCCKPPT CGFTF
Sbjct: 121 LRRRVQRSYKWERIRTCLSTTTICPELNQRYTLAQDFFNAHLDPIQSGCCKPPTKCGFTF 180

Query: 181 INPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 240
           +NPTYWISPI   A  DC  WSN+Q  LCY CDSCKAGLLAN+K +W  AD+ LL+AL+G
Sbjct: 181 VNPTYWISPIDMSADMDCLNWSNDQNTLCYTCDSCKAGLLANIKVDWLKADIFLLLALIG 240

Query: 241 LVFVYLMAFFALKNAKSEDLFRRHKQGYT 270
           L+ VY++   A +NA++ED+FR++KQGYT
Sbjct: 241 LIIVYIIGCCAFRNAETEDIFRKYKQGYT 269

BLAST of Lsi07G011840 vs. TAIR 10
Match: AT2G19580.1 (tetraspanin2 )

HSP 1 Score: 281.6 bits (719), Expect = 6.8e-76
Identity = 138/269 (51.30%), Postives = 191/269 (71.00%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGF 60
           MAL+NN+ A +N +ALL SIP+  +GIWLA++ DN CV +L+WPV+VLG++I +V+  GF
Sbjct: 1   MALANNLTAILNLLALLCSIPITASGIWLASKPDNECVNLLRWPVVVLGVLILVVSATGF 60

Query: 61  IGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLW 120
           IGA+     LL  YL  M +LI LL  ++IF F VT     +  P R Y EY L+ FS W
Sbjct: 61  IGAYKYKETLLAVYLCCMAILIGLLLVVLIFAFVVTRPDGSYRVPGRGYKEYRLEGFSNW 120

Query: 121 LQRRVRSSHKWERIRNCLSSTTTCAELNQNYRLAQDFF-NAHITPFQSGCCKPPTDCGFT 180
           L+  V  S  W R+R CL+ T  C +LNQ +  A  FF ++ ITP QSGCCKPPT CG+ 
Sbjct: 121 LKENVVDSKNWGRLRACLADTNVCPKLNQEFITADQFFSSSKITPLQSGCCKPPTACGYN 180

Query: 181 FINPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALL 240
           F+NPT W++P +  A  DC  WSN+Q++LCY C+SCKAGLL NL++EWR A+LIL+I ++
Sbjct: 181 FVNPTLWLNPTNMAADADCYLWSNDQSQLCYNCNSCKAGLLGNLRKEWRKANLILIITVV 240

Query: 241 GLVFVYLMAFFALKNAKSEDLFRRHKQGY 269
            L++VY++A  A +NA++EDLFR++KQG+
Sbjct: 241 VLIWVYVIACSAFRNAQTEDLFRKYKQGW 269

BLAST of Lsi07G011840 vs. TAIR 10
Match: AT3G45600.1 (tetraspanin3 )

HSP 1 Score: 231.1 bits (588), Expect = 1.0e-60
Identity = 117/265 (44.15%), Postives = 176/265 (66.42%), Query Frame = 0

Query: 1   MALSNNVIAAINFIALLLSIPVIGAGIWLATEADNA-CVQILQWPVIVLGIVIFLVALAG 60
           M  SN++I  +NF+  LLSIP++G GIWL++ A++  C++ LQWP+IV+GI I +V+LAG
Sbjct: 1   MRTSNHLIGLVNFLTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIVIGISIMVVSLAG 60

Query: 61  FIGAFWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSL 120
           F GA +R  +L+  YL+ ML++I  L   +IF + VT +GSG T  +R YL+Y+L+D+S 
Sbjct: 61  FAGACYRNKFLMWLYLVVMLLIIAALIGFIIFAYAVTDKGSGRTVLNRGYLDYYLEDYSG 120

Query: 121 WLQRRVRSSHKWERIRNCLSSTTTCAELNQNY----RLAQDFFNAHITPFQSGCCKPPTD 180
           WL+ RV     W +I +CL  +  C ++ +N+      A  FF   ++P +SGCCKPPTD
Sbjct: 121 WLKDRVSDDSYWGKISSCLRDSGACRKIGRNFNGVPETADMFFLRRLSPVESGCCKPPTD 180

Query: 181 CGFTFINPTYWISPISN-GASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLIL 240
           CGF+++N T W +     G + DC  WSN+Q+ LCY C SCKAG+L +LK+ WR   +I 
Sbjct: 181 CGFSYVNETGWDTRGGMIGPNQDCMVWSNDQSMLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

Query: 241 LIALLGLVFVYLMAFFALKNAKSED 260
           ++ L+ LV  Y++A+ A +N K  D
Sbjct: 241 IVVLIILVIFYVIAYAAYRNVKRID 265

BLAST of Lsi07G011840 vs. TAIR 10
Match: AT2G23810.1 (tetraspanin8 )

HSP 1 Score: 218.4 bits (555), Expect = 7.0e-57
Identity = 104/263 (39.54%), Postives = 159/263 (60.46%), Query Frame = 0

Query: 4   SNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGFIGA 63
           SNN++  +NF+  LLSIP++  GIWL+ +    C + L  PVI LG+ + +VA+AG IG+
Sbjct: 5   SNNLVGILNFLVFLLSIPILAGGIWLSQKGSTECERFLDKPVIALGVFLMVVAIAGLIGS 64

Query: 64  FWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLWLQR 123
             R++WLL  YL  M +LI+L+  + +F F VT +G+G     + Y EY L D+S WLQ+
Sbjct: 65  CCRVTWLLWVYLFVMFLLILLVFCITVFAFVVTNKGAGEAIEGKGYKEYKLGDYSTWLQK 124

Query: 124 RVRSSHKWERIRNCLSSTTTCAELNQNY--RLAQDFFNAHITPFQSGCCKPPTDCGFTFI 183
           RV +   W +IR+CL  +  C++L   +       F+  H+T  QSGCCKP  +CGF ++
Sbjct: 125 RVENGKNWNKIRSCLVESKVCSKLEAKFVNVPVNSFYKEHLTALQSGCCKPSDECGFEYV 184

Query: 184 NPTYWI-SPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLG 243
           NPT W  +      + DC  W N + +LC+ C SCKAGLL N+K  W+   ++ ++ L+ 
Sbjct: 185 NPTTWTKNTTGTHTNPDCQTWDNAKEKLCFDCQSCKAGLLDNVKSAWKKVAIVNIVFLVF 244

Query: 244 LVFVYLMAFFALKNAKSEDLFRR 264
           L+ VY +   A +N K +D + R
Sbjct: 245 LIIVYSVGCCAFRNNKRDDSYSR 267

BLAST of Lsi07G011840 vs. TAIR 10
Match: AT4G28050.1 (tetraspanin7 )

HSP 1 Score: 216.9 bits (551), Expect = 2.0e-56
Identity = 108/253 (42.69%), Postives = 153/253 (60.47%), Query Frame = 0

Query: 4   SNNVIAAINFIALLLSIPVIGAGIWLATEADNACVQILQWPVIVLGIVIFLVALAGFIGA 63
           SNN++  +NF   LLSIP++ AGIWL   A   C + L  P++VLGI +  V++AG +GA
Sbjct: 5   SNNLLGILNFFTFLLSIPILSAGIWLGKNAATECERFLDKPMVVLGIFLMFVSIAGLVGA 64

Query: 64  FWRISWLLMFYLIAMLVLIILLGSLVIFVFFVTMRGSGHTEPSRAYLEYHLDDFSLWLQR 123
             R+S LL  YL AM +LI+L     IF F VT RG+G     R Y EYH+ D+S WLQ+
Sbjct: 65  CCRVSCLLWLYLFAMFLLILLGFCFTIFAFAVTNRGAGEVISDRGYKEYHVADYSNWLQK 124

Query: 124 RVRSSHKWERIRNCLSSTTTCAELNQNYRL--AQDFFNAHITPFQSGCCKPPTDCGFTFI 183
           RV ++  WERIR+CL  +  C+     Y     +DF+ +++   QSGCCKP  DC FT++
Sbjct: 125 RVNNAKNWERIRSCLMYSDVCSTYRTRYASINVEDFYKSNLNALQSGCCKPSNDCNFTYV 184

Query: 184 NPTYWISPISNGASTDCSKWSNEQTELCYGCDSCKAGLLANLKEEWRNADLILLIALLGL 243
           NPT W        + DC+ W N+   LCY C++CKAGLL N+K  W+    + ++ L+ L
Sbjct: 185 NPTTWTKTPGPYKNEDCNVWDNKPGTLCYDCEACKAGLLDNIKNSWKKVAKVNIVFLIFL 244

Query: 244 VFVYLMAFFALKN 255
           + VY +   A +N
Sbjct: 245 IIVYSVGCCAFRN 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIQ55.0e-10869.52Protein TORNADO 2 OS=Arabidopsis thaliana OX=3702 GN=TRN2 PE=1 SV=1[more]
Q9ZUN59.5e-7551.30Tetraspanin-2 OS=Arabidopsis thaliana OX=3702 GN=TET2 PE=2 SV=1[more]
Q9M1E71.5e-5944.15Tetraspanin-3 OS=Arabidopsis thaliana OX=3702 GN=TET3 PE=1 SV=1[more]
Q8S8Q69.9e-5639.54Tetraspanin-8 OS=Arabidopsis thaliana OX=3702 GN=TET8 PE=2 SV=1[more]
Q9SUD42.9e-5542.69Tetraspanin-7 OS=Arabidopsis thaliana OX=3702 GN=TET7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EBD86.7e-14093.28protein TORNADO 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431037 PE=3 SV=1[more]
A0A1S3BL481.1e-13992.16protein TORNADO 2-like OS=Cucumis melo OX=3656 GN=LOC103490773 PE=3 SV=1[more]
A0A0A0L3X44.4e-13991.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G009500 PE=3 SV=1[more]
A0A6J1HK715.7e-13993.28protein TORNADO 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465262 PE=4 SV=1[more]
A0A5N6R6781.5e-11875.84Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_012453 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038905565.13.3e-14193.31protein TORNADO 2 [Benincasa hispida][more]
XP_023552937.11.1e-13993.66protein TORNADO 2 [Cucurbita pepo subsp. pepo][more]
XP_022923295.11.4e-13993.28protein TORNADO 2-like [Cucurbita moschata][more]
XP_008448672.12.4e-13992.16PREDICTED: protein TORNADO 2-like [Cucumis melo][more]
KAG7015753.13.1e-13993.28Protein TORNADO 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G46700.13.6e-10969.52Tetraspanin family protein [more]
AT2G19580.16.8e-7651.30tetraspanin2 [more]
AT3G45600.11.0e-6044.15tetraspanin3 [more]
AT2G23810.17.0e-5739.54tetraspanin8 [more]
AT4G28050.12.0e-5642.69tetraspanin7 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00259TMFOURcoord: 6..29
score: 25.09
coord: 38..64
score: 26.56
coord: 65..93
score: 17.53
NoneNo IPR availablePANTHERPTHR32191:SF14PROTEIN TORNADO 2coord: 1..267
IPR018499Tetraspanin/PeripherinPFAMPF00335Tetraspanincoord: 7..246
e-value: 7.3E-28
score: 97.8
IPR044991Tetraspani, plantPANTHERPTHR32191TETRASPANIN-8-RELATEDcoord: 1..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G011840.1Lsi07G011840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0010305 leaf vascular tissue pattern formation
biological_process GO:0009554 megasporogenesis
biological_process GO:0009956 radial pattern formation
biological_process GO:0009934 regulation of meristem structural organization
biological_process GO:0010015 root morphogenesis
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009506 plasmodesma