Lsi07G008530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G008530
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionheme-binding protein 2
Locationchr07: 9596976 .. 9598632 (+)
RNA-Seq ExpressionLsi07G008530
SyntenyLsi07G008530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAATTTTATGGAAACCATATCCAATTAAGTGAAAAAAATTGAAAAGAACGAGTGGACGTCCTTCACTCTTACACTCTCCGTGGCTTATCTTTTTATGCCTCTTTCTCTCTCTTTCGCTCATCCTTCTTACGAGTTTCGACTGCTAGAATTCCGCCATGGAAATGGAAATGGTGAAGCTGTGGGTAGTGCTAAGCCTTGTGATGGTGATCCTGAGGCCTGCGTACTCGGAGTTTCCTCCGACCTGCAACCGCATCGAGTGCCCTAGCTACGACGTCATCGACTCCGGCGATGGGTTCGAGATTCGGCGCTACGATTCCCCTGTTTGGGCCTCCACCACTCCCATTTCCGACATCTCTCTCCGTGAAGCCACCAGAGCAGGCTTTCTCCAGTAACGCTACTTCTCCCCTTCTCTTCCTTCCCAATTCTTTCTTTTATTATCTAATTTTACTTCCTTCTCACTTGGATGCTTCCATTAGTTTTTCTCCAATTTCTCTAAACCCTAACTTGTCATCAAAACTCATCTTTCCTTATGCATAATATTTCATCATTCACCAATAATTGACTTTGTAATTTACAAACCCATTTACTCTAAATCTTAACATTAAAATCTAGAGACTTCAACGAACTAGATTGTTACTCGACACATTTGAACCTAAAAAAGAAACTAAGTTAGGTAATAATCTTAATATTTAGGAGTGAATTTAGTATGGAGTTATGAGTAGCAATTTTAAGAATAATAACTAAGTGTATGACACTATTTTAAAAAAAAAAATATAAATATAGAAAATTTATCAATGATAGACTTCTATTGCTGATAGACCATCTATGCCATAAAAGTCTACATCGATATTGTTATATATGCTAATTCCGGACACTCATGGATATAAAAAAAAAAAATTCATTAGCCATAAAATTTAATTTTATATTTAATTTGTAAAATTCTGTAATATTTGTAAGACTTGTTTGACACAAAATTTATGAGTATTACCTTGAATTTTTGGCATGTGATTCACAAAATTTATGTTTTTCTTTGAAATTGTTGGTTTGTGTCTTTTAATTTGATGTCTAATAGATTCATTACATTGTCTGATTTACTTTCTTTCCTTAGATTGTTCAATTACATTCAAGGAAAGAACAGTTACAATGAAAAGATAGAAATGACTGCACCGGTGATCACAGAGCTTTCTCCGAGCGATGGACCGTTTTGTGAATCATCATTTGTCGTTAGCTTCTACGTGCCGAAAGTGAACCAAGCAGATCCACCAGGAGCCAAAGGGCTTCATGTACAGAGATGGAACACTACATATGTTGCAGTTAGACAATTTGGAGGATTTGTTTCTGATGACAATATTGGAGGTGAAGCTGCTGCTTTGGATGAAAGTGTTTTTGACACAAAGTGGGGTGCAGCAATCAGCAAGAGCCGTGGTTCTGCCAGTGCTTCCATTTATACTGTGGCTCAGTACAATTCCCCTTTTGAGTTTGAAGATCGAGTCAATGAGATTTGGTTCTTGTTTGATATTCAAGATGTAGCTGCTCAGGCTTACAAACAATTTTAGTGTTTAATGCTTAAATAAAACTTTTTCTCTTCTTGTTCTTCATAAATCCTTCAATAAAATATGCTTACTTCCCTCTCTATTAGGGAGTTCTAGACATTGA

mRNA sequence

GGAATTTTATGGAAACCATATCCAATTAAGTGAAAAAAATTGAAAAGAACGAGTGGACGTCCTTCACTCTTACACTCTCCGTGGCTTATCTTTTTATGCCTCTTTCTCTCTCTTTCGCTCATCCTTCTTACGAGTTTCGACTGCTAGAATTCCGCCATGGAAATGGAAATGGTGAAGCTGTGGGTAGTGCTAAGCCTTGTGATGGTGATCCTGAGGCCTGCGTACTCGGAGTTTCCTCCGACCTGCAACCGCATCGAGTGCCCTAGCTACGACGTCATCGACTCCGGCGATGGGTTCGAGATTCGGCGCTACGATTCCCCTGTTTGGGCCTCCACCACTCCCATTTCCGACATCTCTCTCCGTGAAGCCACCAGAGCAGGCTTTCTCCAATTGTTCAATTACATTCAAGGAAAGAACAGTTACAATGAAAAGATAGAAATGACTGCACCGGTGATCACAGAGCTTTCTCCGAGCGATGGACCGTTTTGTGAATCATCATTTGTCGTTAGCTTCTACGTGCCGAAAGTGAACCAAGCAGATCCACCAGGAGCCAAAGGGCTTCATGTACAGAGATGGAACACTACATATGTTGCAGTTAGACAATTTGGAGGATTTGTTTCTGATGACAATATTGGAGGTGAAGCTGCTGCTTTGGATGAAAGTGTTTTTGACACAAAGTGGGGTGCAGCAATCAGCAAGAGCCGTGGTTCTGCCAGTGCTTCCATTTATACTGTGGCTCAGTACAATTCCCCTTTTGAGTTTGAAGATCGAGTCAATGAGATTTGGTTCTTGTTTGATATTCAAGATGTAGCTGCTCAGGCTTACAAACAATTTTAGTGTTTAATGCTTAAATAAAACTTTTTCTCTTCTTGTTCTTCATAAATCCTTCAATAAAATATGCTTACTTCCCTCTCTATTAGGGAGTTCTAGACATTGA

Coding sequence (CDS)

ATGGAAATGGAAATGGTGAAGCTGTGGGTAGTGCTAAGCCTTGTGATGGTGATCCTGAGGCCTGCGTACTCGGAGTTTCCTCCGACCTGCAACCGCATCGAGTGCCCTAGCTACGACGTCATCGACTCCGGCGATGGGTTCGAGATTCGGCGCTACGATTCCCCTGTTTGGGCCTCCACCACTCCCATTTCCGACATCTCTCTCCGTGAAGCCACCAGAGCAGGCTTTCTCCAATTGTTCAATTACATTCAAGGAAAGAACAGTTACAATGAAAAGATAGAAATGACTGCACCGGTGATCACAGAGCTTTCTCCGAGCGATGGACCGTTTTGTGAATCATCATTTGTCGTTAGCTTCTACGTGCCGAAAGTGAACCAAGCAGATCCACCAGGAGCCAAAGGGCTTCATGTACAGAGATGGAACACTACATATGTTGCAGTTAGACAATTTGGAGGATTTGTTTCTGATGACAATATTGGAGGTGAAGCTGCTGCTTTGGATGAAAGTGTTTTTGACACAAAGTGGGGTGCAGCAATCAGCAAGAGCCGTGGTTCTGCCAGTGCTTCCATTTATACTGTGGCTCAGTACAATTCCCCTTTTGAGTTTGAAGATCGAGTCAATGAGATTTGGTTCTTGTTTGATATTCAAGATGTAGCTGCTCAGGCTTACAAACAATTTTAG

Protein sequence

MEMEMVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTPISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVPKVNQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRGSASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQAYKQF
Homology
BLAST of Lsi07G008530 vs. ExPASy Swiss-Prot
Match: Q9Y5Z4 (Heme-binding protein 2 OS=Homo sapiens OX=9606 GN=HEBP2 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-19
Identity = 60/174 (34.48%), Postives = 82/174 (47.13%), Query Frame = 0

Query: 47  FEIRRYDSPVWASTTPISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPS 106
           +EIR Y    W ST+ +  +    A + GF +L +YIQGKN    KI+MTAPV + + P 
Sbjct: 38  YEIRHYGPAKWVSTS-VESMDWDSAIQTGFTKLNSYIQGKNEKEMKIKMTAPVTSYVEPG 97

Query: 107 DGPFCESSFVVSFYVPKVNQADPPG--AKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAA 166
            GPF ES+  +S Y+P   Q DPP      + ++      V VR F GF S      +  
Sbjct: 98  SGPFSESTITISLYIPSEQQFDPPRPLESDVFIEDRAEMTVFVRSFDGFSSAQKNQEQLL 157

Query: 167 AL------DESVFDTKWGAAISKSRGSASASIYTVAQYNSPFEFEDRVNEIWFL 213
            L      D  VFD K               +Y  A YNSP +  +R NE+W +
Sbjct: 158 TLASILREDGKVFDEK---------------VYYTAGYNSPVKLLNRNNEVWLI 195

BLAST of Lsi07G008530 vs. ExPASy Swiss-Prot
Match: Q9WU63 (Heme-binding protein 2 OS=Mus musculus OX=10090 GN=Hebp2 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-19
Identity = 60/187 (32.09%), Postives = 87/187 (46.52%), Query Frame = 0

Query: 33  IECPSY----DVIDSGDGFEIRRYDSPVWASTTPISDISLREATRAGFLQLFNYIQGKNS 92
           +E PS+    D+      +EIR Y    W ST  +  +    A + GF +L  YIQGKN 
Sbjct: 20  LEMPSWKAPEDIDPQPGSYEIRHYGPAKWVSTC-VESLDWDSAIQTGFTKLNGYIQGKNE 79

Query: 93  YNEKIEMTAPVITELSPSDGPFCESSFVVSFYVPKVNQADP--PGAKGLHVQRWNTTYVA 152
              KI++TAPV + + P   PF ES+  +S Y+P   Q DP  P    + ++      V 
Sbjct: 80  KEMKIKLTAPVTSYVEPGSSPFSESTITISLYIPSEQQPDPPRPSESDVFIEDRAEMTVF 139

Query: 153 VRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRGSA-SASIYTVAQYNSPFEFEDR 212
           VR F GF S      +   L          A I +  G   +  ++  A Y+SPF+  DR
Sbjct: 140 VRSFDGFSSGQKNQEQLLTL----------ANILREEGKVFNEKVFYTAGYSSPFQLLDR 195

BLAST of Lsi07G008530 vs. ExPASy Swiss-Prot
Match: Q9SR77 (Heme-binding-like protein At3g10130, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g10130 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.1e-06
Identity = 51/184 (27.72%), Postives = 79/184 (42.93%), Query Frame = 0

Query: 33  IECPSYDVIDSGDGFEIRRYDSPVWASTTPISDISLRE-ATRAGFLQLFNYIQGKNSYNE 92
           +E  ++ V+   D +EIR+ +    A T    +           F  L  Y+ GKN+  E
Sbjct: 114 LETMNFRVLFRTDKYEIRQVEPYFVAETIMPGETGFDSYGASKSFNVLAEYLFGKNTIKE 173

Query: 93  KIEMTAPVITELSPSDGPFCE-------------SSFVVSFYVPKVNQADPPGAK--GLH 152
           K+EMT PV+T    S G   E             + + +SF +P    ++ P  K   + 
Sbjct: 174 KMEMTTPVVTRKVQSVGEKMEMTTPVITSKAKDQNQWRMSFVMPSKYGSNLPLPKDPSVK 233

Query: 153 VQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRGSASASIYTVAQY 201
           +Q+     VAV  F G+V+D+ I      L  ++ + K      K R     S + VAQY
Sbjct: 234 IQQVPRKIVAVVAFSGYVTDEEIERRERELRRALQNDK------KFRVRDGVS-FEVAQY 290

BLAST of Lsi07G008530 vs. ExPASy TrEMBL
Match: A0A0A0KVD6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G075210 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.0e-109
Identity = 193/220 (87.73%), Postives = 207/220 (94.09%), Query Frame = 0

Query: 3   MEMVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTP 62
           MEM KLWVV+SLVM+IL PAYSEFPPTC RIECPSYDVI +GDGFEIRRY+SPVWAST P
Sbjct: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60

Query: 63  ISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVP 122
           I DISLREATRAGFLQLF+YIQGKNS+NEKIEMT PVITE+SPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120

Query: 123 KVNQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKS 182
           K+NQADPP AKGLH+QRWN+TYVAVRQFGGFV+D NIG EA+ALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180

Query: 183 RGSASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQA 223
           RG+A  SIYTVAQYNSPFEFE RVNEIWFLFDI+DVAAQA
Sbjct: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 220

BLAST of Lsi07G008530 vs. ExPASy TrEMBL
Match: A0A5A7V4M9 (Heme-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001260 PE=3 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 2.8e-107
Identity = 192/220 (87.27%), Postives = 205/220 (93.18%), Query Frame = 0

Query: 3   MEMVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTP 62
           MEM +LWVV SLVMVIL PAYSEFPPTC RIECPSYDVI++GDGFEIRRY+SPVWAST P
Sbjct: 1   MEMGRLWVV-SLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 63  ISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVP 122
           I DISL EATRAGFLQLF+YIQGKN YNEKIEMT PVITE+SPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 123 KVNQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKS 182
           K+NQADPP AKGLH+QRWN+TYVAVRQFGGFV+D NIG EAAALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 183 RGSASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQA 223
           RG+A  S+YTVAQYNSPFEFE RVNEIWFLFDI+DVAAQA
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 219

BLAST of Lsi07G008530 vs. ExPASy TrEMBL
Match: A0A1S3BSD4 (heme-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103492972 PE=3 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 2.8e-107
Identity = 192/220 (87.27%), Postives = 205/220 (93.18%), Query Frame = 0

Query: 3   MEMVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTP 62
           MEM +LWVV SLVMVIL PAYSEFPPTC RIECPSYDVI++GDGFEIRRY+SPVWAST P
Sbjct: 1   MEMGRLWVV-SLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 63  ISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVP 122
           I DISL EATRAGFLQLF+YIQGKN YNEKIEMT PVITE+SPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 123 KVNQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKS 182
           K+NQADPP AKGLH+QRWN+TYVAVRQFGGFV+D NIG EAAALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 183 RGSASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQA 223
           RG+A  S+YTVAQYNSPFEFE RVNEIWFLFDI+DVAAQA
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 219

BLAST of Lsi07G008530 vs. ExPASy TrEMBL
Match: A0A6J1EZ63 (heme-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437561 PE=3 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 8.8e-101
Identity = 179/218 (82.11%), Postives = 196/218 (89.91%), Query Frame = 0

Query: 5   MVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTPIS 64
           +VKL ++ +L+++ILRPAYSE PP+C RIECPSYDVIDSG GFEIRRYDSPVWAST PI 
Sbjct: 6   IVKLSLLATLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSPVWASTAPIP 65

Query: 65  DISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVPKV 124
           DISL EATR GFL+LF+YIQGKN YNEKIEMTAPV+TE+SPSDGPFCESSFVVSFYVPKV
Sbjct: 66  DISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVLTEVSPSDGPFCESSFVVSFYVPKV 125

Query: 125 NQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRG 184
           NQADPP AKGLH+QRW  TYVAVRQFGGFVSD  IG EAAALDESV+DTKWGAAISKSRG
Sbjct: 126 NQADPPPAKGLHIQRWKETYVAVRQFGGFVSDATIGAEAAALDESVYDTKWGAAISKSRG 185

Query: 185 SASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQA 223
           +  A+ YTVAQYNSPFEFE RVNEIWFLFDI+D AA A
Sbjct: 186 AGGAATYTVAQYNSPFEFEGRVNEIWFLFDIEDAAAAA 223

BLAST of Lsi07G008530 vs. ExPASy TrEMBL
Match: A0A6J1J8G7 (heme-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482697 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.5e-100
Identity = 180/223 (80.72%), Postives = 198/223 (88.79%), Query Frame = 0

Query: 3   MEMVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTP 62
           +EM+KL ++ +L+++ILRPAYSE PP+C RIECPSYDVIDSG GFEIRRYDS VWAST P
Sbjct: 6   IEMLKLSLLTTLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSSVWASTAP 65

Query: 63  ISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVP 122
           I DISL EATR GFL+LF+YIQGKN YNEKIEMTAPV+TE+SPSDGPFCESSFVVSFYVP
Sbjct: 66  IPDISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVLTEVSPSDGPFCESSFVVSFYVP 125

Query: 123 KVNQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKS 182
           KVNQADPP AKGLH+QRW  T+VAVRQFGGFVSD  IG EAAALDESVFDTKW AAISKS
Sbjct: 126 KVNQADPPPAKGLHIQRWKETHVAVRQFGGFVSDATIGAEAAALDESVFDTKWAAAISKS 185

Query: 183 RGSASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQAYKQ 226
           RG+  A+IYTVAQYNSPFEFE RVNEIWFLFDI+D AA A  Q
Sbjct: 186 RGAGGAAIYTVAQYNSPFEFEGRVNEIWFLFDIEDAAADAAAQ 228

BLAST of Lsi07G008530 vs. NCBI nr
Match: XP_004137252.1 (heme-binding protein 2 [Cucumis sativus] >KGN53540.1 hypothetical protein Csa_015165 [Cucumis sativus])

HSP 1 Score: 404.4 bits (1038), Expect = 6.2e-109
Identity = 193/220 (87.73%), Postives = 207/220 (94.09%), Query Frame = 0

Query: 3   MEMVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTP 62
           MEM KLWVV+SLVM+IL PAYSEFPPTC RIECPSYDVI +GDGFEIRRY+SPVWAST P
Sbjct: 1   MEMAKLWVVVSLVMMILTPAYSEFPPTCKRIECPSYDVIGTGDGFEIRRYNSPVWASTAP 60

Query: 63  ISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVP 122
           I DISLREATRAGFLQLF+YIQGKNS+NEKIEMT PVITE+SPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLREATRAGFLQLFDYIQGKNSFNEKIEMTGPVITEISPSDGPFCESSFVVSFYVP 120

Query: 123 KVNQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKS 182
           K+NQADPP AKGLH+QRWN+TYVAVRQFGGFV+D NIG EA+ALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEASALDESVFDTKWGAAISKS 180

Query: 183 RGSASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQA 223
           RG+A  SIYTVAQYNSPFEFE RVNEIWFLFDI+DVAAQA
Sbjct: 181 RGAAGPSIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 220

BLAST of Lsi07G008530 vs. NCBI nr
Match: XP_038903399.1 (heme-binding protein 2-like [Benincasa hispida])

HSP 1 Score: 399.1 bits (1024), Expect = 2.6e-107
Identity = 196/217 (90.32%), Postives = 205/217 (94.47%), Query Frame = 0

Query: 5   MVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTPIS 64
           MVKLW VLSLVMVIL PAYS+FPPTCNRIECP+YDVIDSGD FEIRRY+SPVWAST PI 
Sbjct: 1   MVKLWGVLSLVMVILMPAYSKFPPTCNRIECPAYDVIDSGDEFEIRRYNSPVWASTAPIP 60

Query: 65  DISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVPKV 124
           DISLREATRAGFLQLF+YIQGKNSYNEKIEMTAPVITE+SPSDGPFCESSFVVSFYVP V
Sbjct: 61  DISLREATRAGFLQLFDYIQGKNSYNEKIEMTAPVITEVSPSDGPFCESSFVVSFYVPGV 120

Query: 125 NQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRG 184
           NQADPP A+GLHVQRWN+TYVAVRQFGGFVSD+NIG EAAALDESVFDTKWGAAISKSRG
Sbjct: 121 NQADPPPARGLHVQRWNSTYVAVRQFGGFVSDENIGSEAAALDESVFDTKWGAAISKSRG 180

Query: 185 SAS-ASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAA 221
             S ASIYTVAQYNSPFEFE RVNEIWFLFDI+DVAA
Sbjct: 181 GGSGASIYTVAQYNSPFEFEGRVNEIWFLFDIEDVAA 217

BLAST of Lsi07G008530 vs. NCBI nr
Match: XP_008451794.1 (PREDICTED: heme-binding protein 2 [Cucumis melo] >KAA0063003.1 heme-binding protein 2 [Cucumis melo var. makuwa] >TYK16344.1 heme-binding protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 397.9 bits (1021), Expect = 5.8e-107
Identity = 192/220 (87.27%), Postives = 205/220 (93.18%), Query Frame = 0

Query: 3   MEMVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTP 62
           MEM +LWVV SLVMVIL PAYSEFPPTC RIECPSYDVI++GDGFEIRRY+SPVWAST P
Sbjct: 1   MEMGRLWVV-SLVMVILTPAYSEFPPTCKRIECPSYDVIETGDGFEIRRYNSPVWASTAP 60

Query: 63  ISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVP 122
           I DISL EATRAGFLQLF+YIQGKN YNEKIEMT PVITE+SPSDGPFCESSFVVSFYVP
Sbjct: 61  IPDISLHEATRAGFLQLFDYIQGKNKYNEKIEMTGPVITEVSPSDGPFCESSFVVSFYVP 120

Query: 123 KVNQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKS 182
           K+NQADPP AKGLH+QRWN+TYVAVRQFGGFV+D NIG EAAALDESVFDTKWGAAISKS
Sbjct: 121 KINQADPPPAKGLHIQRWNSTYVAVRQFGGFVTDANIGSEAAALDESVFDTKWGAAISKS 180

Query: 183 RGSASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQA 223
           RG+A  S+YTVAQYNSPFEFE RVNEIWFLFDI+DVAAQA
Sbjct: 181 RGAAGPSLYTVAQYNSPFEFEGRVNEIWFLFDIEDVAAQA 219

BLAST of Lsi07G008530 vs. NCBI nr
Match: XP_023552168.1 (heme-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 379.0 bits (972), Expect = 2.8e-101
Identity = 181/218 (83.03%), Postives = 197/218 (90.37%), Query Frame = 0

Query: 5   MVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTPIS 64
           +VKL ++ +L+++ILRPAYSE PP+C RIECPSYDVIDSG GFEIRRYDSPVWAST PI 
Sbjct: 6   IVKLSLLATLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSPVWASTAPIP 65

Query: 65  DISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVPKV 124
           DISL EATR GFL+LF+YIQGKN YNEKIEMTAPVITE+SPSDGPFCESSFVVSFYVPKV
Sbjct: 66  DISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVITEVSPSDGPFCESSFVVSFYVPKV 125

Query: 125 NQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRG 184
           NQADPP AKGLH+QRW  TYVAVRQFGGFVSD  IG EAAALDESV+DTKWGAAISKSRG
Sbjct: 126 NQADPPPAKGLHIQRWKETYVAVRQFGGFVSDATIGAEAAALDESVYDTKWGAAISKSRG 185

Query: 185 SASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQA 223
           +  A+IYTVAQYNSPFEFE RVNEIWFLFDI+D AA A
Sbjct: 186 AGGAAIYTVAQYNSPFEFEGRVNEIWFLFDIEDAAAAA 223

BLAST of Lsi07G008530 vs. NCBI nr
Match: KAG6577056.1 (Heme-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377.1 bits (967), Expect = 1.1e-100
Identity = 180/221 (81.45%), Postives = 197/221 (89.14%), Query Frame = 0

Query: 5   MVKLWVVLSLVMVILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTPIS 64
           +VKL ++ +L+++ILRPAYSE PP+C RIECPSYDVIDSG GFEIRRYDSPVWAST PI 
Sbjct: 6   IVKLSLLATLILLILRPAYSELPPSCKRIECPSYDVIDSGKGFEIRRYDSPVWASTAPIP 65

Query: 65  DISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVPKV 124
           DISL EATR GFL+LF+YIQGKN YNEKIEMTAPV+TE+SPSDGPFCESSFVVSFYVPKV
Sbjct: 66  DISLHEATRTGFLKLFDYIQGKNKYNEKIEMTAPVLTEVSPSDGPFCESSFVVSFYVPKV 125

Query: 125 NQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRG 184
           NQADPP AKGLH+QRW  TYVAVRQFGGFVSD  IG EAAALDESV+DTKWGAAISKSRG
Sbjct: 126 NQADPPPAKGLHIQRWKETYVAVRQFGGFVSDATIGAEAAALDESVYDTKWGAAISKSRG 185

Query: 185 SASASIYTVAQYNSPFEFEDRVNEIWFLFDIQDVAAQAYKQ 226
           +  A+ YTVAQYNSPFEFE RVNEIWFLFDI+D AA A  Q
Sbjct: 186 AGGAATYTVAQYNSPFEFEGRVNEIWFLFDIEDAAAAAAAQ 226

BLAST of Lsi07G008530 vs. TAIR 10
Match: AT1G17100.1 (SOUL heme-binding family protein )

HSP 1 Score: 278.9 bits (712), Expect = 3.7e-75
Identity = 130/194 (67.01%), Postives = 157/194 (80.93%), Query Frame = 0

Query: 25  EFPPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTPISDISLREATRAGFLQLFNYIQ 84
           +FPP+CNRIECPSY+++ SG+G+EIRRY++ VW ST PI DISL +ATR  F QLF YIQ
Sbjct: 37  KFPPSCNRIECPSYELVHSGNGYEIRRYNNTVWVSTEPIPDISLVDATRTAFFQLFAYIQ 96

Query: 85  GKNSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVPKVNQADPPGAKGLHVQRWNTTY 144
           GKN Y++KIEMTAPVI+++SPSDGPFCESSF VSFYVPK NQ DP  ++ LH+Q+WN+ Y
Sbjct: 97  GKNEYHQKIEMTAPVISQVSPSDGPFCESSFTVSFYVPKKNQPDPAPSENLHIQKWNSRY 156

Query: 145 VAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSR---GSASASIYTVAQYNSPFE 204
           VAVRQF GFVSDD+IG +AAALD S+  T W  AI+KS+   G  S S YTVAQYNSPFE
Sbjct: 157 VAVRQFSGFVSDDSIGEQAAALDSSLKGTAWANAIAKSKEDGGVGSDSAYTVAQYNSPFE 216

Query: 205 FEDRVNEIWFLFDI 216
           F  RVNEIW  F++
Sbjct: 217 FSGRVNEIWLPFEL 230

BLAST of Lsi07G008530 vs. TAIR 10
Match: AT1G78450.1 (SOUL heme-binding family protein )

HSP 1 Score: 206.5 bits (524), Expect = 2.3e-53
Identity = 99/194 (51.03%), Postives = 134/194 (69.07%), Query Frame = 0

Query: 27  PPTCNRIECPSYDVIDSGDGFEIRRYDSPVWASTTPISDISLREATRAGFLQLFNYIQGK 86
           PP CNR ECPSY+V+ +G+G+EI RY++ VW ST PI DISL EA+  G+ QL +Y+ G 
Sbjct: 28  PPNCNRAECPSYEVVHAGNGYEIHRYNTTVWISTEPIQDISLNEASGNGWNQLSDYMNGN 87

Query: 87  NSYNEKIEMTAPVITELSPSDGPFCESSFVVSFYVPKVNQADPPGAKGLHVQRWNTTYVA 146
           N Y+++IE+  P IT++S +      S+F+VSF+VPK  Q DPP    LHVQRW++ YVA
Sbjct: 88  NDYHQRIEIALPYITQVSQN-----LSTFIVSFFVPKAFQPDPPPGNNLHVQRWDSRYVA 147

Query: 147 VRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSR---GSASASIYTVAQYNSPFEFE 206
           V+Q  G+V+D  IG + A L  S+  T W  AI KSR   G  SA  YTVAQ++ PF++ 
Sbjct: 148 VKQISGYVADHKIGKQVAELKASLQGTVWAKAIEKSRETGGVGSAWAYTVAQFSWPFQWS 207

Query: 207 DRVNEIWFLFDIQD 218
            RVNEIWF F+++D
Sbjct: 208 QRVNEIWFPFEMED 216

BLAST of Lsi07G008530 vs. TAIR 10
Match: AT1G78460.1 (SOUL heme-binding family protein )

HSP 1 Score: 166.4 bits (420), Expect = 2.7e-41
Identity = 96/222 (43.24%), Postives = 134/222 (60.36%), Query Frame = 0

Query: 3   MEMVKLWVVLSLVMV--------ILRPAYSEFPPTCNRIECPSYDVIDSGDGFEIRRYDS 62
           + ++KL + +SLV+V           P+    P TC+  ECP+Y ++++G GFEIR YD+
Sbjct: 5   LNILKLSLCVSLVVVGSYAQAPAPWNPSNGFRPGTCDHYECPTYKLVEAGYGFEIRMYDA 64

Query: 63  PVWASTTPISDISLREATRAGFLQLFNYIQGKNSYNEKIEMTAPVITELSPSDGPFCESS 122
            +W ST+PI  +S+ +AT+ GF +L  YI+G N  N K+ MTAPVI + +P       S 
Sbjct: 65  ALWISTSPIPSLSMTQATKTGFRRLNRYIEGDNKSNVKMNMTAPVIAQATPG-----RSV 124

Query: 123 FVVSFYVPKVNQADPPGAKGLHVQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTK 182
           + VS Y+PK NQ +PP A  LHV+    TYVAVRQ GG+VS++    EAAAL ES+ D+ 
Sbjct: 125 YTVSLYLPKKNQQNPPQADDLHVRSTKPTYVAVRQIGGYVSNNVAKDEAAALMESLRDSN 184

Query: 183 WGAAISKSRGSASASIYTVAQYNSPFEFEDRV-NEIWFLFDI 216
           W   I KS+G   A  Y +A YN P     RV NEI   F++
Sbjct: 185 WILPIEKSKGKLPA--YFLAVYNPPSHTTARVINEIMVPFNM 219

BLAST of Lsi07G008530 vs. TAIR 10
Match: AT3G10130.1 (SOUL heme-binding family protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.5e-07
Identity = 51/184 (27.72%), Postives = 79/184 (42.93%), Query Frame = 0

Query: 33  IECPSYDVIDSGDGFEIRRYDSPVWASTTPISDISLRE-ATRAGFLQLFNYIQGKNSYNE 92
           +E  ++ V+   D +EIR+ +    A T    +           F  L  Y+ GKN+  E
Sbjct: 114 LETMNFRVLFRTDKYEIRQVEPYFVAETIMPGETGFDSYGASKSFNVLAEYLFGKNTIKE 173

Query: 93  KIEMTAPVITELSPSDGPFCE-------------SSFVVSFYVPKVNQADPPGAK--GLH 152
           K+EMT PV+T    S G   E             + + +SF +P    ++ P  K   + 
Sbjct: 174 KMEMTTPVVTRKVQSVGEKMEMTTPVITSKAKDQNQWRMSFVMPSKYGSNLPLPKDPSVK 233

Query: 153 VQRWNTTYVAVRQFGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRGSASASIYTVAQY 201
           +Q+     VAV  F G+V+D+ I      L  ++ + K      K R     S + VAQY
Sbjct: 234 IQQVPRKIVAVVAFSGYVTDEEIERRERELRRALQNDK------KFRVRDGVS-FEVAQY 290

BLAST of Lsi07G008530 vs. TAIR 10
Match: AT5G20140.1 (SOUL heme-binding family protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.3e-06
Identity = 41/170 (24.12%), Postives = 74/170 (43.53%), Query Frame = 0

Query: 33  IECPSYDVIDSGDGFEIRRYDSPVWASTTPISDISLREATRAGFLQLFNYIQGKNSYNEK 92
           +E P Y ++     +E+R Y+  +   T     I  + +  +GF  +  YI GKNS  EK
Sbjct: 205 LETPKYQILKRTANYEVRNYEPFIVVET-----IGDKLSGSSGFNNVAGYIFGKNSTMEK 264

Query: 93  IEMTAPVITELSPSDGPFCESSFVVSFYVPK---VNQADPPGAKGLHVQRWNTTYVAVRQ 152
           I MT PV T+ + +      S   V   +P    ++    P  + +++++    + A  +
Sbjct: 265 IPMTTPVFTQTTDTQ---LSSDVSVQIVIPSGKDLSSLPMPNEEKVNLKKLEGGFAAAVK 324

Query: 153 FGGFVSDDNIGGEAAALDESVFDTKWGAAISKSRGSASASIYTVAQYNSP 200
           F G  ++D +  +   L  S+  +K G    K           +A+YN P
Sbjct: 325 FSGKPTEDVVQAKENELRSSL--SKDGLRAKKG--------CMLARYNDP 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9Y5Z41.3e-1934.48Heme-binding protein 2 OS=Homo sapiens OX=9606 GN=HEBP2 PE=1 SV=1[more]
Q9WU631.6e-1932.09Heme-binding protein 2 OS=Mus musculus OX=10090 GN=Hebp2 PE=1 SV=1[more]
Q9SR772.1e-0627.72Heme-binding-like protein At3g10130, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A0A0KVD63.0e-10987.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G075210 PE=3 SV=1[more]
A0A5A7V4M92.8e-10787.27Heme-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A1S3BSD42.8e-10787.27heme-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103492972 PE=3 SV=1[more]
A0A6J1EZ638.8e-10182.11heme-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437561 PE=3 S... [more]
A0A6J1J8G71.5e-10080.72heme-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482697 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_004137252.16.2e-10987.73heme-binding protein 2 [Cucumis sativus] >KGN53540.1 hypothetical protein Csa_01... [more]
XP_038903399.12.6e-10790.32heme-binding protein 2-like [Benincasa hispida][more]
XP_008451794.15.8e-10787.27PREDICTED: heme-binding protein 2 [Cucumis melo] >KAA0063003.1 heme-binding prot... [more]
XP_023552168.12.8e-10183.03heme-binding protein 2-like [Cucurbita pepo subsp. pepo][more]
KAG6577056.11.1e-10081.45Heme-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G17100.13.7e-7567.01SOUL heme-binding family protein [more]
AT1G78450.12.3e-5351.03SOUL heme-binding family protein [more]
AT1G78460.12.7e-4143.24SOUL heme-binding family protein [more]
AT3G10130.11.5e-0727.72SOUL heme-binding family protein [more]
AT5G20140.11.3e-0624.12SOUL heme-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006917SOUL haem-binding proteinPFAMPF04832SOULcoord: 33..211
e-value: 1.9E-45
score: 155.1
IPR006917SOUL haem-binding proteinPANTHERPTHR11220HEME-BINDING PROTEIN-RELATEDcoord: 7..219
IPR011256Regulatory factor, effector binding domain superfamilyGENE3D3.20.80.10Regulatory factor, effector binding domaincoord: 32..221
e-value: 3.6E-52
score: 178.7
IPR011256Regulatory factor, effector binding domain superfamilySUPERFAMILY55136Probable bacterial effector-binding domaincoord: 26..213
NoneNo IPR availablePANTHERPTHR11220:SF25F3F9.4coord: 7..219

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G008530.1Lsi07G008530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity