Lsi06G011310 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G011310
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	nicotianamine synthase-like
Location	chr06: 21563174 .. 21564031 (-)
RNA-Seq Expression	Lsi06G011310
Synteny	Lsi06G011310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCCCTCCAAATCTATAGTACCGAAACCGAAATCTCTGCGGAGTTTTTGGTAGATCGAATCATCGACCTTTACGAAAGCATCTCGAAGCTCGAAAGCCTAAAGCCATGCAAGAAAGTGAACAACCTCTTCACCAACCTAGTAAACTTGTGCATTTTGCCTTGTTCAATTGATGTCTCAACATTGCCACCAAATTTACAAGCCATTCGTGAGAGGCTCATAGTTCTATGTGGCCAAGCTGAGGGCTTACTCGAGTTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAACCTCTCAACAATCTCACTCTATTTCCATATTATCAAAACTACATCAAATTAGCCAACCTTGAAAACAAGATTTTGAATGAAAATGGAGTTGTCAATCCAAAGAAGGTAGCTTTTGTTGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAACACATGAAGGGCACATATTTCGATAACTACGATGTAGACAACGTGGCAAATGACGTGGCGGGAAAGATCGTGGGGTCAGATGCTGAGGTGGAAGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAAAAGAAGAGCTTGGAGGATATGATTGTGTGTTTTTAGCGGCTCTTGTGGGAATGAAGAAGGAAGATAAAGTGAAGATTATAAGGCATTTAAGAAAGTATATGAAAGAAGGTGGGATTTTGGTAGTGAGAAGTGCTAAAGGAGGTAGAGCATTTTTATACCCTGTGGTTGAAGTTGGAGATTTGGTTGGCTTTGAGATTCTATCTATTTTTCATCCAACTGATGATGTTGTTAATTCGGTTATTTTAGCTCGCAAGACTATGATTATATGA

mRNA sequence

ATGGCAGCCCTCCAAATCTATAGTACCGAAACCGAAATCTCTGCGGAGTTTTTGGTAGATCGAATCATCGACCTTTACGAAAGCATCTCGAAGCTCGAAAGCCTAAAGCCATGCAAGAAAGTGAACAACCTCTTCACCAACCTAGTAAACTTGTGCATTTTGCCTTGTTCAATTGATGTCTCAACATTGCCACCAAATTTACAAGCCATTCGTGAGAGGCTCATAGTTCTATGTGGCCAAGCTGAGGGCTTACTCGAGTTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAACCTCTCAACAATCTCACTCTATTTCCATATTATCAAAACTACATCAAATTAGCCAACCTTGAAAACAAGATTTTGAATGAAAATGGAGTTGTCAATCCAAAGAAGGTAGCTTTTGTTGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAACACATGAAGGGCACATATTTCGATAACTACGATGTAGACAACGTGGCAAATGACGTGGCGGGAAAGATCGTGGGGTCAGATGCTGAGGTGGAAGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAAAAGAAGAGCTTGGAGGATATGATTGTGTGTTTTTAGCGGCTCTTGTGGGAATGAAGAAGGAAGATAAAGTGAAGATTATAAGGCATTTAAGAAAGTATATGAAAGAAGGTGGGATTTTGGTAGTGAGAAGTGCTAAAGGAGGTAGAGCATTTTTATACCCTGTGGTTGAAGTTGGAGATTTGGTTGGCTTTGAGATTCTATCTATTTTTCATCCAACTGATGATGTTGTTAATTCGGTTATTTTAGCTCGCAAGACTATGATTATATGA

Coding sequence (CDS)

ATGGCAGCCCTCCAAATCTATAGTACCGAAACCGAAATCTCTGCGGAGTTTTTGGTAGATCGAATCATCGACCTTTACGAAAGCATCTCGAAGCTCGAAAGCCTAAAGCCATGCAAGAAAGTGAACAACCTCTTCACCAACCTAGTAAACTTGTGCATTTTGCCTTGTTCAATTGATGTCTCAACATTGCCACCAAATTTACAAGCCATTCGTGAGAGGCTCATAGTTCTATGTGGCCAAGCTGAGGGCTTACTCGAGTTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAACCTCTCAACAATCTCACTCTATTTCCATATTATCAAAACTACATCAAATTAGCCAACCTTGAAAACAAGATTTTGAATGAAAATGGAGTTGTCAATCCAAAGAAGGTAGCTTTTGTTGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAACACATGAAGGGCACATATTTCGATAACTACGATGTAGACAACGTGGCAAATGACGTGGCGGGAAAGATCGTGGGGTCAGATGCTGAGGTGGAAGGAAGAATGAAGTTTTGGACGAGGGATATTGTTGAGGTAAAAGAAGAGCTTGGAGGATATGATTGTGTGTTTTTAGCGGCTCTTGTGGGAATGAAGAAGGAAGATAAAGTGAAGATTATAAGGCATTTAAGAAAGTATATGAAAGAAGGTGGGATTTTGGTAGTGAGAAGTGCTAAAGGAGGTAGAGCATTTTTATACCCTGTGGTTGAAGTTGGAGATTTGGTTGGCTTTGAGATTCTATCTATTTTTCATCCAACTGATGATGTTGTTAATTCGGTTATTTTAGCTCGCAAGACTATGATTATATGA

Protein sequence

MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDVSTLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLENKILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARKTMII

Homology

[Close]

BLAST of Lsi06G011310 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.3e-79
Identity = 149/264 (56.44%), Postives = 194/264 (73.48%), Query Frame = 0


Query: 18  LVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDVSTLPPNLQAIRERLIVL 77
           +V+++ +LYE IS+LE+L P K VN LFT+LV+ C+ P  IDVS L   +Q IR  LI L
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNPIDVSKLCQKIQEIRSHLIKL 68

Query: 78  CGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLENKILNENGVVNPKKVAFV 137
           CGQAEGLLE  FS +LS    PL +L +FPY+ NYIKL+ LE  IL +N    PKK+AF+
Sbjct: 69  CGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIAFI 128

Query: 138 GSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTRDIVEVK 197
           GSGPLPLTS+++A +H+K T F NYD+D  AN +A  +V +D ++  RM F T D+++V 
Sbjct: 129 GSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVT 188

Query: 198 EELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEVGDL 257
             L  YD VFLAALVGM KEDKVK++ HL KYM  G  L++RSA G RAFLYPV++  DL
Sbjct: 189 CALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPRDL 248

Query: 258 VGFEILSIFHPTDDVVNSVILARK 282
            GFE+LS++HPTD+V+NSVI+ARK
Sbjct: 249 RGFEVLSVYHPTDEVINSVIIARK 272

[Close]

BLAST of Lsi06G011310 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 8.3e-77
Identity = 146/268 (54.48%), Postives = 201/268 (75.00%), Query Frame = 0


Query: 18  LVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRERLIV 77
           LV++I DLYE ISKLE+LKPC+ V+ LF  LV+ CI P  +IDV+ +  N+Q +R  LI 
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 78  LCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKIL--NENGVVNPKK 137
           +CG+AEG LE  FS++L+     PL++L LFPYY NY+KL+ LE  +L  N NG V P+ 
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV-PRT 128

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTRDI 197
           VAF+GSGPLPLTS+++A  H+K + F N+D+D  AN VA ++V SD ++  RM F T DI
Sbjct: 129 VAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDI 188

Query: 198 VEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVE 257
           ++V E L G+D VFLAALVGM K++KVK++ HL K+M  G +L++RSA G RAFLYP+VE
Sbjct: 189 MDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVE 248

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GFE+LS++HPTD+V+NS++++RK
Sbjct: 249 PCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Lsi06G011310 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.3e-74
Identity = 152/274 (55.47%), Postives = 197/274 (71.90%), Query Frame = 0


Query: 16  EFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRERL 75
           E LV  I DLYE ISKLESLKP + VN LF  LV+ CI P  +IDV+ +   +Q IR  L
Sbjct: 6   EQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNL 65

Query: 76  IVLCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKIL--NENGVVNP 135
           I +CG AEG LE  FS++L+     PL++L +FPYY NY+KL  LE  +L  N NG V P
Sbjct: 66  IKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV-P 125

Query: 136 KKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTR 195
           K VAF+GSGPLPLTSI++A  H+K T F N+D+D  AN +A  +V SD ++  RM F T 
Sbjct: 126 KSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTV 185

Query: 196 DIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPV 255
           DI++V E L  +D VFLAALVGM KE+KVK+I HL+K+M  G +L++RSA G RAFLYP+
Sbjct: 186 DIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPI 245

Query: 256 VEVGDLVGFEILSIFHPTDDVVNSVILARKTMII 286
           VE  DL GFE+LSI+HPTDDV+NSV++++K  ++
Sbjct: 246 VEPCDLQGFEVLSIYHPTDDVINSVVISKKHPVV 278

[Close]

BLAST of Lsi06G011310 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 6.8e-71
Identity = 138/268 (51.49%), Postives = 189/268 (70.52%), Query Frame = 0


Query: 18  LVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCI-LPCSIDVSTLPPNLQAIRERLIV 77
           +V +IIDLY+ ISKL+SLKP K V+ LF  LV+ C+    +IDV+ +   ++ +R  LI 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGQAEGLLELEFSTLLSKIPK---PLNNLTLFPYYQNYIKLANLENKILNENGVVNPKK 137
           LCG+AEG LE  FST+L  + +   PL++L +FPYY NY+KL  LE  +L+++    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTRDI 197
           +AFVGSGP+PLTSI++A  H+  T F N+D+D+ AN +A  +V  D ++  RM F T D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVE 257
           +   E L  YD VFLAALVGM KE KVK I HL K+M  G +L++RSA   RAFLYP+V+
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GF++L+I+HPTDDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of Lsi06G011310 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 5.7e-70
Identity = 138/267 (51.69%), Postives = 187/267 (70.04%), Query Frame = 0


Query: 18  LVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCI-LPCSIDVSTL-PPNLQAIRERLI 77
           +V +I+DLY  IS LESLKP K V+ LF  LV+ C+    +IDV+ +    ++ +R  LI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 78  VLCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKILNENGVVNPKKV 137
            LCG+AEG LE  FS +L      PLN+L +FPYY NY+KL  LE  +L+++    P KV
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 138 AFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTRDIV 197
           AF+GSGP+PLTSI++A  H+  T F N+D+D+ AN +A  +V  D+++  RM F T D++
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 198 EVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEV 257
             KE L  YD VFLAALVGM KE KVK I HL K+M  G ++++RSA G RAFLYP+V+ 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 258 GDLVGFEILSIFHPTDDVVNSVILARK 282
            DL GFE+L+I+HP+DDVVNSV++ARK
Sbjct: 248 CDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Lsi06G011310 vs. ExPASy TrEMBL
Match: A0A5D3CIT2 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00680 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 4.2e-140
Identity = 254/281 (90.39%), Postives = 270/281 (96.09%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ +  E EISA FLVDRIIDL++SI KLE+L+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRE LI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KILN+NG+VNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVD+VANDVA KIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           E+EGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 282
           A+GGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Lsi06G011310 vs. ExPASy TrEMBL
Match: A0A1S3BXH0 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 4.2e-140
Identity = 254/281 (90.39%), Postives = 270/281 (96.09%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ +  E EISA FLVDRIIDL++SI KLE+L+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRE LI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KILN+NG+VNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVD+VANDVA KIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           E+EGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 282
           A+GGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Lsi06G011310 vs. ExPASy TrEMBL
Match: A0A5A7UXS8 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006240 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.2e-139
Identity = 253/281 (90.04%), Postives = 270/281 (96.09%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ +  E EISA FLVDRIIDL++SI KLE+L+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRE LI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KILN+NG+VNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVD+VANDVA KIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           E+EGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 282
           A+GGRAFLYPVV+VGDLVGFEILSIFHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Lsi06G011310 vs. ExPASy TrEMBL
Match: A0A0A0LUJ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 7.9e-139
Identity = 253/281 (90.04%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQI+ TE EISA FLVDRIIDL+ESISKLE+L+PCKKVNNLFTNLV LCILPCSIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQ IRE LI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KILN+NG+VNPKKVAFVGSGPLPLTSIIMAMQHMKGT+FDNYDVD+VANDVA KIVGSD+
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ++EGRMKF + DIV+VKEELGGYDCVFLAALVGM KE+KVKIIRHLRKYMKEGGIL+VRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 282
           AKGGRAFLYPVVEV DLVGFEILSIFHPTDDVVNSVIL RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of Lsi06G011310 vs. ExPASy TrEMBL
Match: A0A6J1JBQ8 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.3e-128
Identity = 232/285 (81.40%), Postives = 261/285 (91.58%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           M ALQI  TET+IS E L+ R+IDL+ SIS+LE+LKPCK+VN LFTNLV  CILPCSIDV
Sbjct: 1   MEALQILGTETDISEEVLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKFCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPP+LQAIRE LI+ CG+AEGLLELEFST+L KIPKPLNNLTLFPYY NYIKLA+LE 
Sbjct: 61  STLPPHLQAIRESLILFCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KIL +NGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYD+D++AN+VAGKIV SD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDIDSMANEVAGKIVASDG 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           E+EGRMKFWTRDIVEV EEL GYDCVFLAALVG+KKEDKVKI+ HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLKKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARKTMII 286
           AKGGRAFLYPVVEV D+VGF+ILSIFHP+DDVVNSV+LARK ++I
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILI 285

[Close]

BLAST of Lsi06G011310 vs. NCBI nr
Match: XP_038880344.1 (nicotianamine synthase-like [Benincasa hispida])

HSP 1 Score: 534.3 bits (1375), Expect = 6.6e-148
Identity = 268/285 (94.04%), Postives = 279/285 (97.89%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQI+ T+TEIS EFLVDRIIDL+ESISKLE+LKPCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRE LIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN
Sbjct: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KILN+NG+ NPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYD+D+VANDVAGKIVGSD 
Sbjct: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           E+EGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARKTMII 286
           AKGGRAFLYPVVEVGDLVGFEILS+FHPTDDVVNSVILARK MI+
Sbjct: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARKPMIM 285

[Close]

BLAST of Lsi06G011310 vs. NCBI nr
Match: XP_008453616.1 (PREDICTED: nicotianamine synthase-like [Cucumis melo] >TYK11847.1 nicotianamine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 507.3 bits (1305), Expect = 8.6e-140
Identity = 254/281 (90.39%), Postives = 270/281 (96.09%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ +  E EISA FLVDRIIDL++SI KLE+L+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRE LI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KILN+NG+VNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVD+VANDVA KIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           E+EGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 282
           A+GGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Lsi06G011310 vs. NCBI nr
Match: KAA0058291.1 (nicotianamine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 505.8 bits (1301), Expect = 2.5e-139
Identity = 253/281 (90.04%), Postives = 270/281 (96.09%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ +  E EISA FLVDRIIDL++SI KLE+L+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQAIRE LI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KILN+NG+VNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVD+VANDVA KIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           E+EGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 282
           A+GGRAFLYPVV+VGDLVGFEILSIFHPTDDVVNSVILARK
Sbjct: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281

[Close]

BLAST of Lsi06G011310 vs. NCBI nr
Match: XP_004146360.2 (probable nicotianamine synthase 4 [Cucumis sativus] >KGN65443.1 hypothetical protein Csa_020027 [Cucumis sativus])

HSP 1 Score: 503.1 bits (1294), Expect = 1.6e-138
Identity = 253/281 (90.04%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQI+ TE EISA FLVDRIIDL+ESISKLE+L+PCKKVNNLFTNLV LCILPCSIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STLPPNLQ IRE LI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KILN+NG+VNPKKVAFVGSGPLPLTSIIMAMQHMKGT+FDNYDVD+VANDVA KIVGSD+
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           ++EGRMKF + DIV+VKEELGGYDCVFLAALVGM KE+KVKIIRHLRKYMKEGGIL+VRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARK 282
           AKGGRAFLYPVVEV DLVGFEILSIFHPTDDVVNSVIL RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of Lsi06G011310 vs. NCBI nr
Match: KAG6589715.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 470.3 bits (1209), Expect = 1.2e-128
Identity = 233/285 (81.75%), Postives = 263/285 (92.28%), Query Frame = 0


Query: 1   MAALQIYSTETEISAEFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQI  TET+IS EFL+ R+IDL+ SIS+LE+LKPCK+VN LFTNLV LCILPCSIDV
Sbjct: 1   MAALQILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLPPNLQAIRERLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120
           STL P+LQAIRE LI+LCG+AEGLLELEFST+L KIPKPLNNLTLFPYY NYIKLA+LE 
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNENGVVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDA 180
           KIL +NGVVNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDVD++AN+VAGKIV SD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 EVEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240
           E+EGRMKFWTRDIVEV EEL GYDCVFLAALVG+ KEDKVKI+ HLRKYMK+GG+LVVRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AKGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARKTMII 286
           A+GGRAFLYPVVEV D+VGF+ILSIFHP+DDVVNSV+LARK +++
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARKPILL 285

[Close]

BLAST of Lsi06G011310 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 288.5 bits (737), Expect = 5.9e-78
Identity = 146/268 (54.48%), Postives = 201/268 (75.00%), Query Frame = 0


Query: 18  LVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRERLIV 77
           LV++I DLYE ISKLE+LKPC+ V+ LF  LV+ CI P  +IDV+ +  N+Q +R  LI 
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 78  LCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKIL--NENGVVNPKK 137
           +CG+AEG LE  FS++L+     PL++L LFPYY NY+KL+ LE  +L  N NG V P+ 
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV-PRT 128

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTRDI 197
           VAF+GSGPLPLTS+++A  H+K + F N+D+D  AN VA ++V SD ++  RM F T DI
Sbjct: 129 VAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDI 188

Query: 198 VEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVE 257
           ++V E L G+D VFLAALVGM K++KVK++ HL K+M  G +L++RSA G RAFLYP+VE
Sbjct: 189 MDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVE 248

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GFE+LS++HPTD+V+NS++++RK
Sbjct: 249 PCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of Lsi06G011310 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 281.2 bits (718), Expect = 9.4e-76
Identity = 152/274 (55.47%), Postives = 197/274 (71.90%), Query Frame = 0


Query: 16  EFLVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRERL 75
           E LV  I DLYE ISKLESLKP + VN LF  LV+ CI P  +IDV+ +   +Q IR  L
Sbjct: 6   EQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNL 65

Query: 76  IVLCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKIL--NENGVVNP 135
           I +CG AEG LE  FS++L+     PL++L +FPYY NY+KL  LE  +L  N NG V P
Sbjct: 66  IKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV-P 125

Query: 136 KKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTR 195
           K VAF+GSGPLPLTSI++A  H+K T F N+D+D  AN +A  +V SD ++  RM F T 
Sbjct: 126 KSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTV 185

Query: 196 DIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPV 255
           DI++V E L  +D VFLAALVGM KE+KVK+I HL+K+M  G +L++RSA G RAFLYP+
Sbjct: 186 DIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPI 245

Query: 256 VEVGDLVGFEILSIFHPTDDVVNSVILARKTMII 286
           VE  DL GFE+LSI+HPTDDV+NSV++++K  ++
Sbjct: 246 VEPCDLQGFEVLSIYHPTDDVINSVVISKKHPVV 278

[Close]

BLAST of Lsi06G011310 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 268.9 bits (686), Expect = 4.8e-72
Identity = 138/268 (51.49%), Postives = 189/268 (70.52%), Query Frame = 0


Query: 18  LVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCI-LPCSIDVSTLPPNLQAIRERLIV 77
           +V +IIDLY+ ISKL+SLKP K V+ LF  LV+ C+    +IDV+ +   ++ +R  LI 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGQAEGLLELEFSTLLSKIPK---PLNNLTLFPYYQNYIKLANLENKILNENGVVNPKK 137
           LCG+AEG LE  FST+L  + +   PL++L +FPYY NY+KL  LE  +L+++    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTRDI 197
           +AFVGSGP+PLTSI++A  H+  T F N+D+D+ AN +A  +V  D ++  RM F T D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVE 257
           +   E L  YD VFLAALVGM KE KVK I HL K+M  G +L++RSA   RAFLYP+V+
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GF++L+I+HPTDDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of Lsi06G011310 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 265.8 bits (678), Expect = 4.1e-71
Identity = 138/267 (51.69%), Postives = 187/267 (70.04%), Query Frame = 0


Query: 18  LVDRIIDLYESISKLESLKPCKKVNNLFTNLVNLCI-LPCSIDVSTL-PPNLQAIRERLI 77
           +V +I+DLY  IS LESLKP K V+ LF  LV+ C+    +IDV+ +    ++ +R  LI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 78  VLCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYIKLANLENKILNENGVVNPKKV 137
            LCG+AEG LE  FS +L      PLN+L +FPYY NY+KL  LE  +L+++    P KV
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 138 AFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDNVANDVAGKIVGSDAEVEGRMKFWTRDIV 197
           AF+GSGP+PLTSI++A  H+  T F N+D+D+ AN +A  +V  D+++  RM F T D++
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 198 EVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRSAKGGRAFLYPVVEV 257
             KE L  YD VFLAALVGM KE KVK I HL K+M  G ++++RSA G RAFLYP+V+ 
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 258 GDLVGFEILSIFHPTDDVVNSVILARK 282
            DL GFE+L+I+HP+DDVVNSV++ARK
Sbjct: 248 CDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of Lsi06G011310 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 55.8 bits (133), Expect = 6.4e-08
Identity = 29/54 (53.70%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 230 MKEGGILVVRSAKGGRAFLYPVVEVGDLVGFEILSIFHP--TDDVVNSVILARK 282
           M  G +L++RSA+G R+FLY  V+  DL GFE+L I+HP  +D  VNSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:

BLAST of Lsi06G011310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	2.3e-79	56.44	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	8.3e-77	54.48	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	1.3e-74	55.47	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	6.8e-71	51.49	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	5.7e-70	51.69	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Lsi06G011310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CIT2	4.2e-140	90.39	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BXH0	4.2e-140	90.39	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1	[more]
A0A5A7UXS8	1.2e-139	90.04	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A0A0LUJ7	7.9e-139	90.04	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1	[more]
A0A6J1JBQ8	1.3e-128	81.40	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111485338 PE=3 SV=...	[more]

BLAST of Lsi06G011310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880344.1	6.6e-148	94.04	nicotianamine synthase-like [Benincasa hispida]	[more]
XP_008453616.1	8.6e-140	90.39	PREDICTED: nicotianamine synthase-like [Cucumis melo] >TYK11847.1 nicotianamine ...	[more]
KAA0058291.1	2.5e-139	90.04	nicotianamine synthase-like [Cucumis melo var. makuwa]	[more]
XP_004146360.2	1.6e-138	90.04	probable nicotianamine synthase 4 [Cucumis sativus] >KGN65443.1 hypothetical pro...	[more]
KAG6589715.1	1.2e-128	81.75	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi06G011310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	5.9e-78	54.48	nicotianamine synthase 4	[more]
AT1G09240.1	9.4e-76	55.47	nicotianamine synthase 3	[more]
AT5G04950.1	4.8e-72	51.49	nicotianamine synthase 1	[more]
AT5G56080.1	4.1e-71	51.69	nicotianamine synthase 2	[more]
AT4G26483.1	6.4e-08	53.70	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 15..283 e-value: 1.7E-106 score: 355.6
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..283
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 10..285 score: 48.623215
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 13..283 e-value: 8.6E-60 score: 204.1
None	No IPR available	PANTHER	PTHR32266:SF11	NICOTIANAMINE SYNTHASE	coord: 1..283
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 116..275

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G011310.1	Lsi06G011310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity