Lsi06G010900 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G010900
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAFG1-like ATPase
Locationchr06: 21190275 .. 21195442 (+)
RNA-Seq ExpressionLsi06G010900
SyntenyLsi06G010900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAATCTTCATATGTAAAAAGAAAAAGGCAAATCACGCTACAACTTCAAAAAAACTTGGAGTTCTCTCTCTTTTTCTCTGAAACTTTTGTAAGCTGCGATTCTTCATCCAGCGTGGATTAAAGGTCACTTCAAATTTCGTAGGCTGCATCGCGAATTCGCCATTAAAGCCTCTCAAGTCTTGCATCCCTTGTCGTTCTCAGCCTTTTCCCGCAACATAAGCTTACCATTCTTGTGCTGGACAATCGGTTCCTGTCTCGCGGTAGGCCGAGATTTCTGGGTTTTGTCGAATCCAGGTTCGTCTTAATACGCCCACGCAACTTTGTTTTGACGATTCGATTTTAATTTCCTGTGTTCAACATGTTTTGAAGGTTTTTTTTTTTTTTTTTTTTTAATCAAAGGGAATCCACCCAATTAAACATTATCTCTGTATTTGATTCCGTGGACATATGTACTCTCGCTTTTTTCTTCTTTTAGTTAAAATAGGAATTCTCTTTCTGTTCGTTTTACTATTTGGTCTACTTTTGACAAAGTGATTTCCAATATTTTGGTTAGTTGGTTCTTAATTTATCTCAATTCATTGATTTCTGTTTTCGATTAGTCATATTTTGGTTAGAAGGCTTTGATTTTTGAACCACTTTGCGCTTGTCCCTTTTATTTGTGTGGCTGTTTATTGGGTTGTGGTCTTTCAGGGTCCTGTCAGAAAAATGAGAGGCGTAAATCGGTATGCTCGTCACCTCCGATCAGTTTTTTCTCAAAAAACACGCAATCATACGGATGTTCTTCGTCGAGGGAAAAGGCAAAGGCAATTGATTCATACCAATTCCGAGTCTCTTTTTATAATTAAACCAGATAGATACAGAACCCCTAAATATATAAACTTAAGAGCTCTGTCAGTTGACGCAGCTAAACTTTCTAATGGAGGTAAGTATCTCTCTCTCCCCCATTAACATAACCTAGCATTCTAAGTTTTATGAGCGAAACCAGATACTTTTGTTTTTAATTTGGACTTTTGGGGTTCAATTGCAGATTATAACGTGGCAGGGCCACTCGTTGAGTATGAACGAAGAATTGCTGCTGGTGAGCTTGTGGATGGTGATAGCTGTCAGGTAAGGCCATCCGTTTCCCCTGCATCTTCATCGTTTATATTTAGAGATGAAAGTGAATTATATATAGAATAATTCATGATAATTCACTTTTGTAAGCTTTATTTTCAGATTTTTACTTTCTGCCTCTGCCTGCTTTTAAAACCAAAATGGCTGATAAACCCAAACAGGAATAAAGAAAATGTGTAATTTTAGCACTACATCTTTGTAAAGCTACTGTTGGTTTATGTTATTAGATTTTGATTTGAGACTATGTTTTTGTTGATAAAATACATGTCTACCCATTTGCGTTAAGCCTGTTGAAATTAATTCTTACAGTCCCCTTGCAGGTAGGGACCCTGAGGGAACTTCAGAGACTCTACGATGAACTTGTTAATTCAGCTGAAGCATGCCGGTTGGATAGATATGCAGTATCAGAAAAATCAGGAAGGTAGTTCTTCCATCATGTAGTTATAGTGCTTGTGTGTACGGATTATTTGAACTTTTTTAATATTTTAGTTTCTTATTTGTCACTAGATTGTTCTTATTATTAATCTTCTTTTTATTGTCTATGCCAGGAGTAGGTGGTTGTGGTCTCGCTTCATTCCTCAGTCATCGTACTCTCCAGTTAAAGGTCTATATCTCTATGGAGGAGTAGGCACTGGAAAAACTATGTTGATGGACTTGTTTTTTGATCAGTTGTAAGTTTGTTGCCTTTATGTTGACAAATGATTCCACAAATTCAAATGATAACCAACTATTATAAGTTCTTTTTGGGATTTAGTTTAGTCCATTTATGCTATCCATTGTCTGTTTTCTCTTACTGGCTCCTTATCTTCGACTTACTCTCTTCTCATAGACCCAGCAATTGGAGGAAAAAGAGGATCCATTTCCATGACTTCATGTTGAACGTTCATAGTCGTTTGCAGGTGAGTTTTATTATTTTCTTCATTAGTGGTGAATGTTTCTAAATGAAATCTACAGTATTGACACTGAGATTATGATGTCTAGTTGTAGTGGAATGTCTTATTTTCTCTGAAATCTCCTAGTTCAGCACGCCCTGTTTTTTTCTTTTTTTTTTTGGTTCCCCCAATGGAGACCTATTGCTTAGTTTGTGTATCTTGTAAAAAAAAATAAAAAATTACTGCTTATTTTGTGTAACTTATAATTGAGAAACCATTTAAATTAGAATTTTTTAGTCATCTGAGTTTTACAATTTGGAAACCAAATATATTTCATAGCCTACTTTTCTGCTCACTCTGTATCCTGGTTGAAGGTAACGACATCAATCTTTTAATGCGTCGGGTATTATCTTTTGATAAACTAATAGTTTGCCTGATCGTGTGCTTTGTGGATATACTAAATACGCATCTGTCTCGACTGCATATTTTGACAGCGGCACAAGGGTGTTGCTGATCCACTTGAAGTTGTTGCAGGAGAGATTTCAGATGAGGCAATTTTATTATGCCTAGATGAATTTATGGTACTCTTTCAATTCAACGGTCTTATTTTTTTTTTTTATTTTTTATATCGTATCTTTTGGCCCTGCACTTTGGTAACTTTAGTAAAATTTCTTATTATTTTGTCTTTTGTAGGTGACAGATGTGGCTGATGCATTGATACTAAATCGTCTGTTTGGACATCTCTTTAGCAATGGCGTTGTAAGTATTTACTTTTACACTTTATTTTTATATTCCAACCATCATAAGATATTGTGCTTGGTTTCAGACATAACTTAGAGATTGTTTTTATCTGATTGAGGGAAATGTAGATCCTGGTTGCAACATCAAATCGCGCTCCAGATAAACTGTATGAAGGTGGATTACAGAGAGATCTTTTCCTGCCCTTCATTTCCACATTGAAGGTATGTTTGTGGACAGTAAAATCCCAGTCATTTTTACCTTTTAGTTCAACAATATAAACGTCAGGACACACTTGAATTAAGCCACCCATTCTAACAATTGTTATCTGAATCTAGTGCCAATTTAGTCGATTTAAGCTCGGTCATTTATGATTTTTCCACTCATTAATATGGAAGGTCTCATGATGTAGGCAAGGTGTGTGACTCATGAAATTGGTTCAGCAACTGACTATCGAAAATTGACTTCGGTAATGATTCTAATTCCATACAGAAGTAGCTATTTGAGCAAAAAGTTCTGAATGATTATATAATATATTTTATACCTACTCTTGACTACAGGCAGAGCGAGGGTTCTACTTTGTTGGAAAGGACTCATCTACCTTTCTTAAGCAGAAGTTTCAACAATTGATCGGTAAAAGTACAGCTGGTCCACAAGAGGTGGAGGTAGTGATGGGCAGGATTCTGCAAGTAAGCACCCAGTTTCATTGTAGGATGATTTGAGTAACTTATTTCTTCAATATTGTTGTTTAGCAATTTTCTCTACTTTTAGGTCCCATCAGGAGCAAATGGATGTGCGTATTTCCCTTTTGAGGAACTCTGTGACAAACCCCTAGGAGCTGCAGATTACTTCGGGTTGTTTAGTAAGTCACTCATTCAAGATGACATTTGAGTTCATATCGGGTAGTAATATTAGGAATGCTGAGTAATATTTTCAATACAAACTTCCAGATTTTTAGGTGTGATTTAAGACTGGATTGTTATAAGCGATAGCTAGTTTGATGAGATGGGTTTATCTTATCCTTAGCTGGAACCACTAACAGTGAATGCATGCTAGGTTTTCAGGAATCATTAACTTCTATTAGCCTGATATTTTGGATTGGAACTCTTTTAGTTAGTCAAATTTCTAGCATCATTCCAAGTGTTGTCATTTACTTTTATTGTGCTAAATTTCTAGTCTAACTTGCTTGTTGGATTTATTTTTAAGAATTTGCTGTCTTCCTTTACAGAAAAATTCCATACCCTGGCATTGGAAGGTATCCCAATATTTGGACTCCACAACAGAACAGCAGCATATCGTTTTGTCACTCTTGTTGATGTATGTCTGACTTCATATTCGGATGCTTTTAACTTCTATAGCCTCACTTTACGAAGAAGTTTGTTTCTTTTGTGACTTCAACCAATATTTATCCATTTGTTTGATGGCCTACAATTCCACTCTTTCCATGTGTTTTTTTTTAATTTTAATTTTATTTTTTATTTTTTATTGTTTAAATTTAAGGCACAGACCAATACTTTGTCAAATATATTAGAAAACGACGGGCCTAGCAATTGGGCAGTGTGATACCAATCAGAACTTATTCTCGTGATTGGTTGATCTCATAAAGGGCATGTTATAAGAGGGGTTCGAATTTGACAGAGACTCGACCCATCCCCTAGGATGGAAACAATTCAAACTAGTTAAAAGAATTTTAGAATTTGAATAGCAAGTGAATTTGGTTCTTTCTCATGCTTTCCTTGTGTAATGACAGGTGATGTATGAAAACAAAGCCAGACTACTATGTACAGCTGAAGGAAGTCCTCATGAACTTTTCGAAAAGATTGTAACCATCTCTGATGCTCAACAAATTGCGCCTAGAACCTCAACACGATCAAGGAAAAATGACGTTTCCGATCTCTGTGTGGACAATGAATTGGGTTTCGCCAAAGACCGTACAATTAGTAGGTAAGGCATCCTCCAAGTATGTTTGGACTTGGAGAATGGAGTTTCTCTCTAGTCAGACATTCATGGCATGTATATATAATTCCTTTTAGTATCCACATGTATATTCATTTTCAGCTGCTAATGTTGATGCCATTTTTGATGTAGATTAACAGAGATAAACAGCAAAGAATACCTGGAACAACATGCTCAACTCGTAGCAGAGAATCGAAACTGGCAAGACGGTGACAATCAAGATGCTTTGCAACCATAATTTCTGCTTCTGCAGCTTCCAGATTAAGCACATATCATTTTTGTCACTGCATTTCACTATTTTAACTTAACTAAAATTATTTACTTTAGTGATAAGAAATAATGATAATTGAAACTTTTATTTTTATACAAACTAACAATTGTTACCCACATATTAGGATGCAGATTGAAGAGTTGGAACATTTTATTATGAGAGTTGTGTAAATGTGAAGGTTGAAATAACAACACCTACTATAATTCTTTACTCAAAAA

mRNA sequence

TTAATCTTCATATGTAAAAAGAAAAAGGCAAATCACGCTACAACTTCAAAAAAACTTGGAGTTCTCTCTCTTTTTCTCTGAAACTTTTGTAAGCTGCGATTCTTCATCCAGCGTGGATTAAAGGTCACTTCAAATTTCGTAGGCTGCATCGCGAATTCGCCATTAAAGCCTCTCAAGTCTTGCATCCCTTGTCGTTCTCAGCCTTTTCCCGCAACATAAGCTTACCATTCTTGTGCTGGACAATCGGTTCCTGTCTCGCGGTAGGCCGAGATTTCTGGGTTTTGTCGAATCCAGGGTCCTGTCAGAAAAATGAGAGGCGTAAATCGGTATGCTCGTCACCTCCGATCAGTTTTTTCTCAAAAAACACGCAATCATACGGATGTTCTTCGTCGAGGGAAAAGGCAAAGGCAATTGATTCATACCAATTCCGAGTCTCTTTTTATAATTAAACCAGATAGATACAGAACCCCTAAATATATAAACTTAAGAGCTCTGTCAGTTGACGCAGCTAAACTTTCTAATGGAGATTATAACGTGGCAGGGCCACTCGTTGAGTATGAACGAAGAATTGCTGCTGGTGAGCTTGTGGATGGTGATAGCTGTCAGGTAGGGACCCTGAGGGAACTTCAGAGACTCTACGATGAACTTGTTAATTCAGCTGAAGCATGCCGGTTGGATAGATATGCAGTATCAGAAAAATCAGGAAGGAGTAGGTGGTTGTGGTCTCGCTTCATTCCTCAGTCATCGTACTCTCCAGTTAAAGGTCTATATCTCTATGGAGGAGTAGGCACTGGAAAAACTATGTTGATGGACTTGTTTTTTGATCAGTTACCCAGCAATTGGAGGAAAAAGAGGATCCATTTCCATGACTTCATGTTGAACGTTCATAGTCGTTTGCAGCGGCACAAGGGTGTTGCTGATCCACTTGAAGTTGTTGCAGGAGAGATTTCAGATGAGGCAATTTTATTATGCCTAGATGAATTTATGGTGACAGATGTGGCTGATGCATTGATACTAAATCGTCTGTTTGGACATCTCTTTAGCAATGGCGTTATCCTGGTTGCAACATCAAATCGCGCTCCAGATAAACTGTATGAAGGTGGATTACAGAGAGATCTTTTCCTGCCCTTCATTTCCACATTGAAGGCAAGGTGTGTGACTCATGAAATTGGTTCAGCAACTGACTATCGAAAATTGACTTCGGCAGAGCGAGGGTTCTACTTTGTTGGAAAGGACTCATCTACCTTTCTTAAGCAGAAGTTTCAACAATTGATCGGTAAAAGTACAGCTGGTCCACAAGAGGTGGAGGTAGTGATGGGCAGGATTCTGCAAGTCCCATCAGGAGCAAATGGATGTGCGTATTTCCCTTTTGAGGAACTCTGTGACAAACCCCTAGGAGCTGCAGATTACTTCGGGTTGTTTAAAAAATTCCATACCCTGGCATTGGAAGGTATCCCAATATTTGGACTCCACAACAGAACAGCAGCATATCGTTTTGTCACTCTTGTTGATGTGATGTATGAAAACAAAGCCAGACTACTATGTACAGCTGAAGGAAGTCCTCATGAACTTTTCGAAAAGATTGTAACCATCTCTGATGCTCAACAAATTGCGCCTAGAACCTCAACACGATCAAGGAAAAATGACGTTTCCGATCTCTGTGTGGACAATGAATTGGGTTTCGCCAAAGACCGTACAATTAGTAGATTAACAGAGATAAACAGCAAAGAATACCTGGAACAACATGCTCAACTCGTAGCAGAGAATCGAAACTGGCAAGACGGTGACAATCAAGATGCTTTGCAACCATAATTTCTGCTTCTGCAGCTTCCAGATTAAGCACATATCATTTTTGTCACTGCATTTCACTATTTTAACTTAACTAAAATTATTTACTTTAGTGATAAGAAATAATGATAATTGAAACTTTTATTTTTATACAAACTAACAATTGTTACCCACATATTAGGATGCAGATTGAAGAGTTGGAACATTTTATTATGAGAGTTGTGTAAATGTGAAGGTTGAAATAACAACACCTACTATAATTCTTTACTCAAAAA

Coding sequence (CDS)

ATGAGAGGCGTAAATCGGTATGCTCGTCACCTCCGATCAGTTTTTTCTCAAAAAACACGCAATCATACGGATGTTCTTCGTCGAGGGAAAAGGCAAAGGCAATTGATTCATACCAATTCCGAGTCTCTTTTTATAATTAAACCAGATAGATACAGAACCCCTAAATATATAAACTTAAGAGCTCTGTCAGTTGACGCAGCTAAACTTTCTAATGGAGATTATAACGTGGCAGGGCCACTCGTTGAGTATGAACGAAGAATTGCTGCTGGTGAGCTTGTGGATGGTGATAGCTGTCAGGTAGGGACCCTGAGGGAACTTCAGAGACTCTACGATGAACTTGTTAATTCAGCTGAAGCATGCCGGTTGGATAGATATGCAGTATCAGAAAAATCAGGAAGGAGTAGGTGGTTGTGGTCTCGCTTCATTCCTCAGTCATCGTACTCTCCAGTTAAAGGTCTATATCTCTATGGAGGAGTAGGCACTGGAAAAACTATGTTGATGGACTTGTTTTTTGATCAGTTACCCAGCAATTGGAGGAAAAAGAGGATCCATTTCCATGACTTCATGTTGAACGTTCATAGTCGTTTGCAGCGGCACAAGGGTGTTGCTGATCCACTTGAAGTTGTTGCAGGAGAGATTTCAGATGAGGCAATTTTATTATGCCTAGATGAATTTATGGTGACAGATGTGGCTGATGCATTGATACTAAATCGTCTGTTTGGACATCTCTTTAGCAATGGCGTTATCCTGGTTGCAACATCAAATCGCGCTCCAGATAAACTGTATGAAGGTGGATTACAGAGAGATCTTTTCCTGCCCTTCATTTCCACATTGAAGGCAAGGTGTGTGACTCATGAAATTGGTTCAGCAACTGACTATCGAAAATTGACTTCGGCAGAGCGAGGGTTCTACTTTGTTGGAAAGGACTCATCTACCTTTCTTAAGCAGAAGTTTCAACAATTGATCGGTAAAAGTACAGCTGGTCCACAAGAGGTGGAGGTAGTGATGGGCAGGATTCTGCAAGTCCCATCAGGAGCAAATGGATGTGCGTATTTCCCTTTTGAGGAACTCTGTGACAAACCCCTAGGAGCTGCAGATTACTTCGGGTTGTTTAAAAAATTCCATACCCTGGCATTGGAAGGTATCCCAATATTTGGACTCCACAACAGAACAGCAGCATATCGTTTTGTCACTCTTGTTGATGTGATGTATGAAAACAAAGCCAGACTACTATGTACAGCTGAAGGAAGTCCTCATGAACTTTTCGAAAAGATTGTAACCATCTCTGATGCTCAACAAATTGCGCCTAGAACCTCAACACGATCAAGGAAAAATGACGTTTCCGATCTCTGTGTGGACAATGAATTGGGTTTCGCCAAAGACCGTACAATTAGTAGATTAACAGAGATAAACAGCAAAGAATACCTGGAACAACATGCTCAACTCGTAGCAGAGAATCGAAACTGGCAAGACGGTGACAATCAAGATGCTTTGCAACCATAA

Protein sequence

MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLRALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEACRLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAERGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHELFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHAQLVAENRNWQDGDNQDALQP
Homology
BLAST of Lsi06G010900 vs. ExPASy Swiss-Prot
Match: Q8WV93 (AFG1-like ATPase OS=Homo sapiens OX=9606 GN=AFG1L PE=1 SV=2)

HSP 1 Score: 263.1 bits (671), Expect = 6.5e-69
Identity = 170/435 (39.08%), Postives = 237/435 (54.48%), Query Frame = 0

Query: 78  GPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEACRLDRYAVSEKSGRSRWL 137
           GPL  Y+  I A EL D D  Q   ++ LQ+L+++L           Y +  +      L
Sbjct: 74  GPLDHYDFLIKAHELKD-DEHQRRVIQCLQKLHEDLKG---------YNIEAEG-----L 133

Query: 138 WSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFMLNVHSRLQ 197
           +S+   +S   P +GLY+YG VGTGKTM+MD+F+  +    RKKR+HFH FML+VH R+ 
Sbjct: 134 FSKLFSRS--KPPRGLYVYGDVGTGKTMVMDMFYAYVEMK-RKKRVHFHGFMLDVHKRIH 193

Query: 198 RHKGVA------------DPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFS 257
           R K               DP+  +A EIS+EA LLC DEF VTD+ADA+IL +LF +LF 
Sbjct: 194 RLKQSLPKRKPGFMAKSYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQLFENLFK 253

Query: 258 NGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRK--LTSAERGF 317
           NGV++VATSNR P+ LY+ GLQR  F+PFI+ LK  C T ++ S  DYRK  L +A + +
Sbjct: 254 NGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSGIDYRKRELPAAGKLY 313

Query: 318 YFVGK-DSSTFLKQKFQQLIGK-STAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKP 377
           Y   + D    + + F +L  K +      +  V GR L++       A   FEELC++P
Sbjct: 314 YLTSEADVEAVMDKLFDELAQKQNDLTRPRILKVQGRELRLNKACGTVADCTFEELCERP 373

Query: 378 LGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHEL 437
           LGA+DY  L K F T+ L  IP F L NRT   RF+TL+D  Y+ K R++C+A      L
Sbjct: 374 LGASDYLELSKNFDTIFLRNIPQFTLANRTQGRRFITLIDNFYDLKVRIICSASTPISSL 433

Query: 438 FEKIVTISDAQQIAPRTSTRSRKNDVSD---LCVDNELGFAKDRTISRLTEINSKEYLEQ 494
           F      S+ +Q            D ++   +    E  FA  RTISRLTE+ +++Y   
Sbjct: 434 FLHQHHDSELEQSRILMDDLGLSQDSAEGLSMFTGEEEIFAFQRTISRLTEMQTEQY--- 477

BLAST of Lsi06G010900 vs. ExPASy Swiss-Prot
Match: Q32PX9 (AFG1-like ATPase OS=Rattus norvegicus OX=10116 GN=Afg1l PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.5e-68
Identity = 167/417 (40.05%), Postives = 232/417 (55.64%), Query Frame = 0

Query: 78  GPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEACRLDRYAVSEKSGRSRWL 137
           GPL  Y+  I A EL   D  Q   ++ LQ+L ++L           Y + E+ G    L
Sbjct: 74  GPLAHYDFLIKAQEL-KNDEHQRRVVQCLQKLQEDLKG---------YNI-EEGGLFSKL 133

Query: 138 WSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFMLNVHSRLQ 197
           +SR        P KGLY+YG VGTGKTM+MD+F++ +     K+R+HFH FML+VH R+ 
Sbjct: 134 FSR------NKPPKGLYVYGDVGTGKTMVMDMFYEYVEVK-SKRRVHFHGFMLDVHKRIH 193

Query: 198 RHKGVA------------DPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFS 257
           R K               DP+  +A EIS EA LLC DEF VTD+ADA+IL +LF +LF 
Sbjct: 194 RLKQSLPKRKVGFMAKSYDPIAPIAEEISQEASLLCFDEFQVTDIADAMILKQLFENLFK 253

Query: 258 NGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRK--LTSAERGF 317
           NGV++VATSNR P+ LY+ GLQR  F+PFI+ LK  C T ++ S  DYRK  LT A + +
Sbjct: 254 NGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSGVDYRKRELTPAGKLY 313

Query: 318 YFVGK-DSSTFLKQKFQQLIGKST--AGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 377
           Y   + D  T + + F +L  K      P+ ++ V GR L++       A   FEELC++
Sbjct: 314 YLTSEADVGTVMDKLFDELAQKQNDLTSPRILK-VQGRELRLNKACGTVADCTFEELCER 373

Query: 378 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 437
           PLGA+DY  L K F T+ +  IP F L  RT   RF+TL+D  Y+ K R++C+A      
Sbjct: 374 PLGASDYLELSKNFDTVIIRNIPQFSLAKRTQVRRFITLIDNFYDFKVRIICSASVPISS 433

Query: 438 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCV--DNELGFAKDRTISRLTEINSKEY 476
           LF      S++ Q            D + L +    E  F+  RT+SRLTE+ +++Y
Sbjct: 434 LFVYQHQDSESDQSRVLMDDLGLSQDSAGLSMFTGEEEIFSFQRTLSRLTEMQTEQY 471

BLAST of Lsi06G010900 vs. ExPASy Swiss-Prot
Match: Q3V384 (AFG1-like ATPase OS=Mus musculus OX=10090 GN=Afg1l PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 9.4e-68
Identity = 168/417 (40.29%), Postives = 236/417 (56.59%), Query Frame = 0

Query: 78  GPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEACRLDRYAVSEKSGRSRWL 137
           GPL  Y+  I + EL + D  Q   ++ LQ+L ++L           Y++ E+ G    L
Sbjct: 74  GPLDHYDFLIKSQELRE-DEHQRRVVQCLQKLQEDLKG---------YSI-EEGGLFSKL 133

Query: 138 WSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFMLNVHSRL- 197
           +SR        P KGLY+YG VGTGKTM+MD+F+  + +  RKKR+HFH FML+VH R+ 
Sbjct: 134 FSR------NKPPKGLYVYGDVGTGKTMVMDMFYAYVETK-RKKRVHFHGFMLDVHRRIH 193

Query: 198 -------QRHKGVA----DPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFS 257
                  +R  G      DP+  +A EIS E  LLC DEF VTD+ADA+IL +LF +LF 
Sbjct: 194 HLKQSLPKRKAGFMAKSYDPIAPIAEEISQETSLLCFDEFQVTDIADAMILKQLFENLFK 253

Query: 258 NGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRK--LTSAERGF 317
           NGV++VATSNR P+ LY+ GLQR  F+PFI+ LK  C T ++ S  DYRK  L  A + +
Sbjct: 254 NGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCDTLQLDSGVDYRKRELAPAGKLY 313

Query: 318 YFVGK-DSSTFLKQKFQQLIGKST--AGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 377
           Y   + D    + + F +L  K      P+ ++ V GR L++       A   FEELC++
Sbjct: 314 YLTSEADVEAVVDKLFDELAQKQNDLTSPRILK-VQGRELRLNKACGSVADCTFEELCER 373

Query: 378 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 437
           PLGA+DY  L K F T+ +  IP F L  RT A RF+TL+D  Y+ K R++C+A      
Sbjct: 374 PLGASDYLELSKNFDTVIIRNIPQFSLAKRTQARRFITLIDNFYDFKVRIICSASAPISS 433

Query: 438 LF--EKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEY 476
           LF  +   + SD  +I       S+ +    +    E  FA  RTISRLTE+ +++Y
Sbjct: 434 LFLHQHQDSESDQSRILMDDLGLSQDSAGLSMFTGEEEIFAFQRTISRLTEMQTEQY 471

BLAST of Lsi06G010900 vs. ExPASy Swiss-Prot
Match: P46441 (Putative ATPase N2B OS=Haematobia irritans OX=7368 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 3.6e-59
Identity = 148/434 (34.10%), Postives = 229/434 (52.76%), Query Frame = 0

Query: 79  PLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEACRLDRYAVSEKSGRSRWLW 138
           P+  YE+RI + EL+  D  Q  T +EL+ LY+ L N           V  ++      +
Sbjct: 37  PMQAYEQRIESKELLP-DKVQKKTTQELEDLYNTLKN------YQPKPVRVETSSGGGFF 96

Query: 139 SRFIPQSSYSP---------VKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFM 198
            RF+ +   +P          KG+Y+YG VG GKT LMD+F+       +K+R+HF+ FM
Sbjct: 97  GRFMKKEQSAPKIELLNTTAPKGMYIYGSVGGGKTTLMDMFYSCCDDIPKKQRVHFNSFM 156

Query: 199 LNVHS---RLQRHKGVA------------DPLEVVAGEISDEAILLCLDEFMVTDVADAL 258
             VH    ++++ +G              DP   VA  I++E+ L+C DEF VTD+ADA+
Sbjct: 157 SKVHGLIHKVKQERGPQDRAFNSEKPLPFDPTLPVAEMIANESWLICFDEFQVTDIADAM 216

Query: 259 ILNRLFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYR 318
           IL  LF HLF+ G++ +ATSNR P+ LY+ GLQR  F+PFI  L  RC    + S  DYR
Sbjct: 217 ILKSLFTHLFNEGIVCIATSNRHPNDLYKNGLQRSNFIPFIGVLLNRCNVAAMDSGVDYR 276

Query: 319 KLTSAERGFYFV--GKDSSTFLKQKFQQLIGKST--AGPQEVEVVMGRILQVPSGANGCA 378
           ++  +    YFV    D+ + +++ F+ L  +      P+ +    GR L          
Sbjct: 277 RIAQSGDTNYFVTTQTDAKSQMERMFKILCSQENDIIRPRTI-THFGRDLTFQRTCGQVL 336

Query: 379 YFPFEELCDKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARL 438
              FEELC++PLG +DY  + + FHT+ +  +P   L  ++   RF+TL+D +Y+N+ R+
Sbjct: 337 DSNFEELCNRPLGGSDYIQIGQFFHTVLIHDVPQLTLLLKSQMRRFITLIDTLYDNRVRV 396

Query: 439 LCTAEGSPHELF---EKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRL 482
           + +AE    +LF   +K   ++D Q++          +  + +    E  FA DRTISRL
Sbjct: 397 VISAEVPLDQLFSFTDKPKDLADEQRMLMDDLKLGDTDTSASVFTGEEEMFAFDRTISRL 456

BLAST of Lsi06G010900 vs. ExPASy Swiss-Prot
Match: Q5TYS0 (Lactation elevated protein 1 homolog B OS=Danio rerio OX=7955 GN=lace1b PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.3e-53
Identity = 133/375 (35.47%), Postives = 197/375 (52.53%), Query Frame = 0

Query: 120 CRLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWR 179
           CR D    +EK  +           S   P +G Y+YG VGTGKTMLMDLF+     N R
Sbjct: 129 CRPDENVSNEKEDQQE-------ESSKPHPPQGYYIYGNVGTGKTMLMDLFY-SFVENRR 188

Query: 180 KKRIHFHDFMLNVHSRLQRHK-----------GVADPLEVVAGEISDEAILLCLDEFMVT 239
           KKR+HF+ FML+VH R+ + K            + DP+  VA EI++E  L+C DEF V 
Sbjct: 189 KKRVHFNGFMLDVHRRIHKLKQSLPKRRIGKMTMYDPIFPVAMEIAEETCLICFDEFQVV 248

Query: 240 DVADALILNRLFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIG 299
           D+ADA+IL +LF  LF  GV++VATSNR P++LY+ GLQR  F+PFI  LK  C    + 
Sbjct: 249 DIADAMILKQLFEGLFKCGVVVVATSNRPPEELYKNGLQRAAFVPFIGVLKEYCRIVSLD 308

Query: 300 SATDY--RKLTSAERGFYFVGK-DSSTFLKQKFQQL-IGKSTAGPQEVEVVMGRILQVPS 359
           +  D+  R++  A R +Y   + D+   +   F++L   ++      V  V GR + +  
Sbjct: 309 TGIDFRTREMKPAGRLYYISSEPDAENAVNALFEELAFRQNDVTRPRVLNVQGREVTLSR 368

Query: 360 GANGCAYFPFEELCDKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMY 419
                A   F+ELC++PLGA DY  + + F T+ +  +P   L  +  A RF TL+D  Y
Sbjct: 369 TCGTIADCSFQELCEQPLGAGDYLEIARCFDTVIIRNVPYLQLGMKDQARRFTTLIDNFY 428

Query: 420 ENKARLLCTAEGSPHELFEKIVTIS----DAQQIAPRTSTRSRKNDVSDLCVDNELGFAK 476
           + K R++  A+     L ++         D   +     T      ++    D E+ FA 
Sbjct: 429 DQKVRVVMLADAPLDRLLDQGQMTGEEARDRLMLDELGLTDEASKRITLFTADEEI-FAF 488

BLAST of Lsi06G010900 vs. ExPASy TrEMBL
Match: A0A5A7SYX6 (Lactation elevated protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00320 PE=3 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 7.5e-278
Identity = 478/500 (95.60%), Postives = 488/500 (97.60%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRYARHLRS FSQKTRNHTDVLRR +RQRQLIHTNSESLFI+KPDRYRTP+ INLR
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRLQRQRQLIHTNSESLFIVKPDRYRTPRCINLR 60

Query: 61  ALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120
            LSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGD+CQVGTLRELQRLYDELVNSAEAC
Sbjct: 61  TLSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDNCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCVTHEIGSATDYRKLTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSS FLKQKFQQLIGKS  G QEVEVVMGR+LQVP GANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSIFLKQKFQQLIGKSIPGRQEVEVVMGRVLQVPLGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420
           PLGAADYFGLFKKFHTLALEG+PIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP+E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGVPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPYE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAENRNWQDGDNQDALQP 501
           +LVAE RNWQ+GD+QDA QP
Sbjct: 481 ELVAEKRNWQNGDDQDASQP 500

BLAST of Lsi06G010900 vs. ExPASy TrEMBL
Match: A0A1S4DV19 (lactation elevated protein 1 OS=Cucumis melo OX=3656 GN=LOC103487322 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.6e-275
Identity = 475/500 (95.00%), Postives = 486/500 (97.20%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRYA HLRS  SQKTRNHTDVLRR +RQRQLIHTNSESLFI+KPDRYRTP+ INLR
Sbjct: 1   MRGVNRYACHLRSAVSQKTRNHTDVLRRLQRQRQLIHTNSESLFIVKPDRYRTPRCINLR 60

Query: 61  ALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120
            LSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGD+CQVGTLRELQRLYDELVNSAEAC
Sbjct: 61  TLSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDNCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCVTHEIGSATDYRKLTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSS FLKQKFQQLIGKS  G QEVEVVMGR+LQVP GANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSIFLKQKFQQLIGKSIPGRQEVEVVMGRVLQVPLGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420
           PLGAADYFGLFKKFHTLALEG+PIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP+E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGVPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPYE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRKNDVS+LCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSNLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAENRNWQDGDNQDALQP 501
           +LVAE RNWQ+GD+QDA QP
Sbjct: 481 ELVAEKRNWQNGDDQDASQP 500

BLAST of Lsi06G010900 vs. ExPASy TrEMBL
Match: A0A6J1JJY1 (AFG1-like ATPase OS=Cucurbita maxima OX=3661 GN=LOC111485772 PE=3 SV=1)

HSP 1 Score: 949.1 bits (2452), Expect = 7.3e-273
Identity = 465/499 (93.19%), Postives = 482/499 (96.59%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRY RHLRS FSQKT N TDVLRRG+RQ+QL+HTNSES FI+K D++R+PKY+NLR
Sbjct: 1   MRGVNRYVRHLRSAFSQKTHNQTDVLRRGQRQKQLVHTNSESFFIVKSDKFRSPKYLNLR 60

Query: 61  ALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120
           ALSVDAAKL NGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC
Sbjct: 61  ALSVDAAKLCNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAE 300
           GHLFSNGVILVATSNRAPD LYEGGLQRDLFLPFISTLK RCVTHEIGSATDYRK+TSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDNLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300

Query: 301 RGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVG  +STFLKQKFQQLIGKSTA P EVEVVMGR+LQVPSGANGCAYFPFEELC+K
Sbjct: 301 RGFYFVGNGASTFLKQKFQQLIGKSTARPHEVEVVMGRVLQVPSGANGCAYFPFEELCNK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRT+AYRFVTLVDVMYENKARLLCTAEGSPHE
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTSAYRFVTLVDVMYENKARLLCTAEGSPHE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEK+VTISDAQQIAPRTS RS+KND+SDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKVVTISDAQQIAPRTSARSQKNDISDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAENRNWQDGDNQDALQ 500
           QLVAE R WQ+ DNQDALQ
Sbjct: 481 QLVAEKRKWQEDDNQDALQ 499

BLAST of Lsi06G010900 vs. ExPASy TrEMBL
Match: A0A0A0LTY3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G277450 PE=3 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 3.1e-271
Identity = 470/500 (94.00%), Postives = 482/500 (96.40%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRYARHLRS FSQKT N TDVLRR +RQRQLIHTNSESLFI+KPDR R   +INLR
Sbjct: 1   MRGVNRYARHLRSAFSQKTCNRTDVLRRVQRQRQLIHTNSESLFIVKPDRNR---FINLR 60

Query: 61  ALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120
            LS+DAAKLSNGDYNVAGPLVEYERRIAAG+LVDGDSCQVGTLRELQRLYDELVNSAEAC
Sbjct: 61  TLSLDAAKLSNGDYNVAGPLVEYERRIAAGDLVDGDSCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCVTHEIGSATDYRKLTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           +GFYFVGKDSS FLKQKFQQLIGKS  GPQEVEVVMGR+LQVP GANGCAYFPFEELCDK
Sbjct: 301 QGFYFVGKDSSIFLKQKFQQLIGKSIPGPQEVEVVMGRVLQVPLGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420
           PLGAADYFGLFKKFHTLAL+G+PIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP E
Sbjct: 361 PLGAADYFGLFKKFHTLALDGVPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           L EKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LLEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAENRNWQDGDNQDALQP 501
           +LVAE RNWQ+GD+QDA QP
Sbjct: 481 ELVAEKRNWQNGDDQDASQP 497

BLAST of Lsi06G010900 vs. ExPASy TrEMBL
Match: A0A6J1C0D0 (AFG1-like ATPase OS=Momordica charantia OX=3673 GN=LOC111007319 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.2e-270
Identity = 468/499 (93.79%), Postives = 480/499 (96.19%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MR VNRY RHLRS FSQ+TR+HT VL RG+RQRQL+HTN  S FI+K DRYRTP+ +  R
Sbjct: 1   MRSVNRYVRHLRSAFSQQTRHHTGVLGRGQRQRQLVHTNCGSAFIVKSDRYRTPEVLISR 60

Query: 61  ALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120
           ALSVDAAKLSNGDY VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDEL+NSAEAC
Sbjct: 61  ALSVDAAKLSNGDYRVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELLNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCVTHEIGSATDYRK+TSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKMTSAE 300

Query: 301 RGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSS+FLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSSFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPQE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRK+DVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKSDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAENRNWQDGDNQDALQ 500
           QLVAE +NWQ   NQDALQ
Sbjct: 481 QLVAEKQNWQGHHNQDALQ 499

BLAST of Lsi06G010900 vs. NCBI nr
Match: XP_038880754.1 (AFG1-like ATPase isoform X4 [Benincasa hispida])

HSP 1 Score: 976.9 bits (2524), Expect = 6.7e-281
Identity = 481/500 (96.20%), Postives = 491/500 (98.20%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRYARHLRS FSQKTRNHTDVLRRG+RQRQLIHTNS SLFI+KPDRYRTPKYINLR
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRGQRQRQLIHTNSGSLFIVKPDRYRTPKYINLR 60

Query: 61  ALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120
            LSVDAAKLSNGDY VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSA+AC
Sbjct: 61  TLSVDAAKLSNGDYKVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAQAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIP SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPHSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCV+HEIGSATDYRKLTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVSHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSS+FLKQKFQQLIGKS AG Q+VEVVMGR+LQVPSGANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSSFLKQKFQQLIGKSIAGSQKVEVVMGRVLQVPSGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRKND+SDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDISDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAENRNWQDGDNQDALQP 501
           +LVAE RNW+DGDNQDALQP
Sbjct: 481 ELVAEKRNWEDGDNQDALQP 500

BLAST of Lsi06G010900 vs. NCBI nr
Match: XP_038880753.1 (AFG1-like ATPase isoform X3 [Benincasa hispida])

HSP 1 Score: 972.2 bits (2512), Expect = 1.7e-279
Identity = 481/501 (96.01%), Postives = 491/501 (98.00%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRYARHLRS FSQKTRNHTDVLRRG+RQRQLIHTNS SLFI+KPDRYRTPKYINLR
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRGQRQRQLIHTNSGSLFIVKPDRYRTPKYINLR 60

Query: 61  ALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120
            LSVDAAKLSNGDY VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSA+AC
Sbjct: 61  TLSVDAAKLSNGDYKVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAQAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIP SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPHSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCV+HEIGSATDYRKLTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVSHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSS+FLKQKFQQLIGKS AG Q+VEVVMGR+LQVPSGANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSSFLKQKFQQLIGKSIAGSQKVEVVMGRVLQVPSGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420
           PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE
Sbjct: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTI-SRLTEINSKEYLEQH 480
           LFEKIVTISDAQQIAPRTSTRSRKND+SDLCVDNELGFAKDRTI SRLTEINSKEYLEQH
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDISDLCVDNELGFAKDRTISSRLTEINSKEYLEQH 480

Query: 481 AQLVAENRNWQDGDNQDALQP 501
           A+LVAE RNW+DGDNQDALQP
Sbjct: 481 AELVAEKRNWEDGDNQDALQP 501

BLAST of Lsi06G010900 vs. NCBI nr
Match: XP_038880752.1 (AFG1-like ATPase isoform X2 [Benincasa hispida])

HSP 1 Score: 971.8 bits (2511), Expect = 2.2e-279
Identity = 481/502 (95.82%), Postives = 491/502 (97.81%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRYARHLRS FSQKTRNHTDVLRRG+RQRQLIHTNS SLFI+KPDRYRTPKYINLR
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRGQRQRQLIHTNSGSLFIVKPDRYRTPKYINLR 60

Query: 61  ALSVDAAKLSNG--DYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAE 120
            LSVDAAKLSNG  DY VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSA+
Sbjct: 61  TLSVDAAKLSNGVADYKVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAQ 120

Query: 121 ACRLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW 180
           ACRLDRYAVSEKSGRSRWLWSRFIP SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW
Sbjct: 121 ACRLDRYAVSEKSGRSRWLWSRFIPHSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW 180

Query: 181 RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR 240
           RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR
Sbjct: 181 RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR 240

Query: 241 LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTS 300
           LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCV+HEIGSATDYRKLTS
Sbjct: 241 LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVSHEIGSATDYRKLTS 300

Query: 301 AERGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELC 360
           AERGFYFVGKDSS+FLKQKFQQLIGKS AG Q+VEVVMGR+LQVPSGANGCAYFPFEELC
Sbjct: 301 AERGFYFVGKDSSSFLKQKFQQLIGKSIAGSQKVEVVMGRVLQVPSGANGCAYFPFEELC 360

Query: 361 DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP 420
           DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP
Sbjct: 361 DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP 420

Query: 421 HELFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQ 480
           HELFEKIVTISDAQQIAPRTSTRSRKND+SDLCVDNELGFAKDRTISRLTEINSKEYLEQ
Sbjct: 421 HELFEKIVTISDAQQIAPRTSTRSRKNDISDLCVDNELGFAKDRTISRLTEINSKEYLEQ 480

Query: 481 HAQLVAENRNWQDGDNQDALQP 501
           HA+LVAE RNW+DGDNQDALQP
Sbjct: 481 HAELVAEKRNWEDGDNQDALQP 502

BLAST of Lsi06G010900 vs. NCBI nr
Match: XP_038880751.1 (AFG1-like ATPase isoform X1 [Benincasa hispida])

HSP 1 Score: 967.2 bits (2499), Expect = 5.3e-278
Identity = 481/503 (95.63%), Postives = 491/503 (97.61%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRYARHLRS FSQKTRNHTDVLRRG+RQRQLIHTNS SLFI+KPDRYRTPKYINLR
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRGQRQRQLIHTNSGSLFIVKPDRYRTPKYINLR 60

Query: 61  ALSVDAAKLSNG--DYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAE 120
            LSVDAAKLSNG  DY VAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSA+
Sbjct: 61  TLSVDAAKLSNGVADYKVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAQ 120

Query: 121 ACRLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW 180
           ACRLDRYAVSEKSGRSRWLWSRFIP SSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW
Sbjct: 121 ACRLDRYAVSEKSGRSRWLWSRFIPHSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNW 180

Query: 181 RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR 240
           RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR
Sbjct: 181 RKKRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNR 240

Query: 241 LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTS 300
           LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCV+HEIGSATDYRKLTS
Sbjct: 241 LFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVSHEIGSATDYRKLTS 300

Query: 301 AERGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELC 360
           AERGFYFVGKDSS+FLKQKFQQLIGKS AG Q+VEVVMGR+LQVPSGANGCAYFPFEELC
Sbjct: 301 AERGFYFVGKDSSSFLKQKFQQLIGKSIAGSQKVEVVMGRVLQVPSGANGCAYFPFEELC 360

Query: 361 DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP 420
           DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP
Sbjct: 361 DKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP 420

Query: 421 HELFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTI-SRLTEINSKEYLE 480
           HELFEKIVTISDAQQIAPRTSTRSRKND+SDLCVDNELGFAKDRTI SRLTEINSKEYLE
Sbjct: 421 HELFEKIVTISDAQQIAPRTSTRSRKNDISDLCVDNELGFAKDRTISSRLTEINSKEYLE 480

Query: 481 QHAQLVAENRNWQDGDNQDALQP 501
           QHA+LVAE RNW+DGDNQDALQP
Sbjct: 481 QHAELVAEKRNWEDGDNQDALQP 503

BLAST of Lsi06G010900 vs. NCBI nr
Match: KAA0035116.1 (lactation elevated protein 1 [Cucumis melo var. makuwa] >TYJ95963.1 lactation elevated protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 965.7 bits (2495), Expect = 1.6e-277
Identity = 478/500 (95.60%), Postives = 488/500 (97.60%), Query Frame = 0

Query: 1   MRGVNRYARHLRSVFSQKTRNHTDVLRRGKRQRQLIHTNSESLFIIKPDRYRTPKYINLR 60
           MRGVNRYARHLRS FSQKTRNHTDVLRR +RQRQLIHTNSESLFI+KPDRYRTP+ INLR
Sbjct: 1   MRGVNRYARHLRSAFSQKTRNHTDVLRRLQRQRQLIHTNSESLFIVKPDRYRTPRCINLR 60

Query: 61  ALSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEAC 120
            LSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGD+CQVGTLRELQRLYDELVNSAEAC
Sbjct: 61  TLSVDAAKLSNGDYNVAGPLVEYERRIAAGELVDGDNCQVGTLRELQRLYDELVNSAEAC 120

Query: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180
           RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK
Sbjct: 121 RLDRYAVSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRK 180

Query: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240
           KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF
Sbjct: 181 KRIHFHDFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLF 240

Query: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAE 300
           GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLK RCVTHEIGSATDYRKLTSAE
Sbjct: 241 GHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKERCVTHEIGSATDYRKLTSAE 300

Query: 301 RGFYFVGKDSSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDK 360
           RGFYFVGKDSS FLKQKFQQLIGKS  G QEVEVVMGR+LQVP GANGCAYFPFEELCDK
Sbjct: 301 RGFYFVGKDSSIFLKQKFQQLIGKSIPGRQEVEVVMGRVLQVPLGANGCAYFPFEELCDK 360

Query: 361 PLGAADYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHE 420
           PLGAADYFGLFKKFHTLALEG+PIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP+E
Sbjct: 361 PLGAADYFGLFKKFHTLALEGVPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPYE 420

Query: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480
           LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA
Sbjct: 421 LFEKIVTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 480

Query: 481 QLVAENRNWQDGDNQDALQP 501
           +LVAE RNWQ+GD+QDA QP
Sbjct: 481 ELVAEKRNWQNGDDQDASQP 500

BLAST of Lsi06G010900 vs. TAIR 10
Match: AT4G28070.2 (AFG1-like ATPase family protein )

HSP 1 Score: 696.8 bits (1797), Expect = 1.3e-200
Identity = 339/418 (81.10%), Postives = 372/418 (89.00%), Query Frame = 0

Query: 70  SNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEACRLDRYAVSE 129
           SNGD  +AGPLVEYERRI AGEL+DGD CQ+GTLRELQRLYDELV SA+ACRLDRY+ S 
Sbjct: 53  SNGDGKIAGPLVEYERRIVAGELLDGDLCQLGTLRELQRLYDELVQSADACRLDRYSASA 112

Query: 130 KSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFM 189
           KS RS W W++F+  SS SPVKGLYLYGGVGTGKTMLMDLFF QLP++WR +RIHFH+FM
Sbjct: 113 KSTRSNWFWNKFVSHSSVSPVKGLYLYGGVGTGKTMLMDLFFHQLPASWRTQRIHFHNFM 172

Query: 190 LNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFSNGVI 249
           L+VHSRLQ+HKG+ DPLEVV  EI+DEAILLCLDEFMV DVADALILNRLF HLF+NG+I
Sbjct: 173 LSVHSRLQKHKGLEDPLEVVGLEIADEAILLCLDEFMVNDVADALILNRLFRHLFNNGII 232

Query: 250 LVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAERGFYFVGKD 309
           LVATSNRAPD LYEGGLQRDLFLPFISTLK RCV  EIGS+ DYRKLTSAE GFYF+GKD
Sbjct: 233 LVATSNRAPDNLYEGGLQRDLFLPFISTLKERCVVREIGSSVDYRKLTSAEEGFYFIGKD 292

Query: 310 SSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKPLGAADYFG 369
            S  LKQKFQ L+G   AGPQ VEVVMGR LQVP  A+GCAYF FEELCD+PLGAADY G
Sbjct: 293 ISGLLKQKFQLLVGDQPAGPQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAADYLG 352

Query: 370 LFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHELFEKIVTIS 429
           LFKKFHTLALEG+P FGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP EL E+IVTIS
Sbjct: 353 LFKKFHTLALEGVPFFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIVTIS 412

Query: 430 DAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHAQLVAENR 488
           DAQQIAPRTS+RSRK+D  DLCVDNELGFAKDRTISRLTE+NSKEYLEQH++++ E +
Sbjct: 413 DAQQIAPRTSSRSRKSDDPDLCVDNELGFAKDRTISRLTEMNSKEYLEQHSRMLQEKQ 470

BLAST of Lsi06G010900 vs. TAIR 10
Match: AT4G30490.1 (AFG1-like ATPase family protein )

HSP 1 Score: 696.4 bits (1796), Expect = 1.6e-200
Identity = 351/475 (73.89%), Postives = 400/475 (84.21%), Query Frame = 0

Query: 13  SVFSQKTRNHTDVLRRGKRQRQ-LIHTNSESLFIIKPDRYRTPKYINLRALSVDAA---- 72
           S F  K+R     L  G  Q Q L++TN+++  +      R+  +  L A +V+AA    
Sbjct: 26  STFYSKSRK----LLIGVNQNQALLNTNTDNSSLYS----RSSIFRGLSAEAVEAADPAT 85

Query: 73  -KLSNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEACRLDRYA 132
            +++  D N  GPLVEYERRI+ GEL+ GD CQ+  LRELQRLYDELV+S + CRLDRY 
Sbjct: 86  TRVTVSDVNRTGPLVEYERRISNGELMTGDICQISALRELQRLYDELVDSVDTCRLDRYN 145

Query: 133 VSEKSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFH 192
            S+KS RSRW WSR +PQ+SYSPVKGLYLYGGVGTGKTMLMDLFFDQLP  W+K+RIHFH
Sbjct: 146 TSDKSSRSRWFWSRLMPQTSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPCTWKKQRIHFH 205

Query: 193 DFMLNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFSN 252
           DFML+VHSRLQ+HKG++DPLEVVA EI+ +AILLCLDEFMVTDVADALILNRLFGHLFSN
Sbjct: 206 DFMLSVHSRLQKHKGLSDPLEVVAQEIAHDAILLCLDEFMVTDVADALILNRLFGHLFSN 265

Query: 253 GVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAERGFYFV 312
           GVILVATSNR PDKLYEGGLQRDLFLPFIS+LK R V HEIGSA DYRKLTSAE+GFYF+
Sbjct: 266 GVILVATSNRNPDKLYEGGLQRDLFLPFISSLKERSVVHEIGSAVDYRKLTSAEQGFYFI 325

Query: 313 GKDSSTFLKQKFQQLIGKS-TAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKPLGAA 372
           GKD ST LKQKF+QLIG +  A PQ VEVVMGR LQ+P GANGCAYFPFEELCD+PLGAA
Sbjct: 326 GKDLSTLLKQKFRQLIGDNVVARPQVVEVVMGRKLQIPLGANGCAYFPFEELCDRPLGAA 385

Query: 373 DYFGLFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHELFEKI 432
           DYFGLFKKFHTLAL+ IP+FGLHNRTAAYRFVTLVDVMYEN+ARLLCTAE +P EL EKI
Sbjct: 386 DYFGLFKKFHTLALDEIPVFGLHNRTAAYRFVTLVDVMYENRARLLCTAEANPQELLEKI 445

Query: 433 VTISDAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHA 481
           +TIS+A+ + PRTS+RSRKNDV++LCVDNELGFAKDRTISRLTE+NSKEYLE HA
Sbjct: 446 ITISEAKSMGPRTSSRSRKNDVTELCVDNELGFAKDRTISRLTEMNSKEYLEHHA 492

BLAST of Lsi06G010900 vs. TAIR 10
Match: AT4G28070.1 (AFG1-like ATPase family protein )

HSP 1 Score: 668.3 bits (1723), Expect = 4.8e-192
Identity = 329/418 (78.71%), Postives = 362/418 (86.60%), Query Frame = 0

Query: 70  SNGDYNVAGPLVEYERRIAAGELVDGDSCQVGTLRELQRLYDELVNSAEACRLDRYAVSE 129
           SNGD  +AGPLVEYERRI AGEL+DGD CQ+GTLRELQRLYDELV SA+ACRLDRY+ S 
Sbjct: 53  SNGDGKIAGPLVEYERRIVAGELLDGDLCQLGTLRELQRLYDELVQSADACRLDRYSASA 112

Query: 130 KSGRSRWLWSRFIPQSSYSPVKGLYLYGGVGTGKTMLMDLFFDQLPSNWRKKRIHFHDFM 189
           KS RS W W++F+  SS SPVKGLYLYGGVGTGKTMLMDLFF QLP++WR +RIHFH+FM
Sbjct: 113 KSTRSNWFWNKFVSHSSVSPVKGLYLYGGVGTGKTMLMDLFFHQLPASWRTQRIHFHNFM 172

Query: 190 LNVHSRLQRHKGVADPLEVVAGEISDEAILLCLDEFMVTDVADALILNRLFGHLFSNGVI 249
           L+VHSRLQ+HKG+ DPLEVV  EI+DEAILLCLDEFMV DVADALILNRLF HLF+NG+I
Sbjct: 173 LSVHSRLQKHKGLEDPLEVVGLEIADEAILLCLDEFMVNDVADALILNRLFRHLFNNGII 232

Query: 250 LVATSNRAPDKLYEGGLQRDLFLPFISTLKARCVTHEIGSATDYRKLTSAERGFYFVGKD 309
           LVATSNRAPD LYEGGLQRDLFLPFISTLK RCV  EIGS+ DYRKLTSAE GFYF+GKD
Sbjct: 233 LVATSNRAPDNLYEGGLQRDLFLPFISTLKERCVVREIGSSVDYRKLTSAEEGFYFIGKD 292

Query: 310 SSTFLKQKFQQLIGKSTAGPQEVEVVMGRILQVPSGANGCAYFPFEELCDKPLGAADYFG 369
            S  LKQKFQ L+G   AGPQ VEVVMGR LQVP  A+GCAYF FEELCD+PLGAADY G
Sbjct: 293 ISGLLKQKFQLLVGDQPAGPQVVEVVMGRKLQVPLAADGCAYFLFEELCDRPLGAADYLG 352

Query: 370 LFKKFHTLALEGIPIFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPHELFEKIVTIS 429
           LF          +P FGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSP EL E+IVTIS
Sbjct: 353 LF---------SVPFFGLHNRTAAYRFVTLVDVMYENKARLLCTAEGSPLELLERIVTIS 412

Query: 430 DAQQIAPRTSTRSRKNDVSDLCVDNELGFAKDRTISRLTEINSKEYLEQHAQLVAENR 488
           DAQQIAPRTS+RSRK+D  DLCVDNELGFAKDRTISRLTE+NSKEYLEQH++++ E +
Sbjct: 413 DAQQIAPRTSSRSRKSDDPDLCVDNELGFAKDRTISRLTEMNSKEYLEQHSRMLQEKQ 461

BLAST of Lsi06G010900 vs. TAIR 10
Match: AT2G25530.1 (AFG1-like ATPase family protein )

HSP 1 Score: 175.6 bits (444), Expect = 9.7e-44
Identity = 115/353 (32.58%), Postives = 174/353 (49.29%), Query Frame = 0

Query: 151 KGLYLYGGVGTGKTMLMDLFFDQLPSNWR-KKRIHFHDFMLNVHSRLQRH---KGVADPL 210
           KGLY+YG VG GKTMLMD+F+       R ++R HFH+ ML ++ ++ ++    G   P+
Sbjct: 242 KGLYIYGNVGCGKTMLMDMFYGATDGIIRHRQRFHFHEAMLKINEQMHKYWKENGAEKPM 301

Query: 211 ------------------EVVAGE---------------ISDE-----------AILLCL 270
                             E +AGE               ++D+           A +LC 
Sbjct: 302 QYSISSWIMNLPVDEKVKEWLAGEEFYKQQLQMKHILPAVADKFLVDQQSSKKGASILCF 361

Query: 271 DEFMVTDVADALILNRLFGHLFSNGVILVATSNRAPDKLYEGGLQRDLFLPFISTLKARC 330
           DE    DV   + L+ +   L + G +LVATSNRAP +L + G+Q+++F  FIS L+  C
Sbjct: 362 DEIQTVDVFAIVALSGIMSRLLTTGTVLVATSNRAPRELNQDGMQKEIFDKFISKLEKHC 421

Query: 331 VTHEIGSATDYRKL---TSAERGFYF------VGKDSSTFLKQKFQQLIGKSTAGPQEVE 390
               IGS  DYR++    SAE   Y       V ++     +Q   Q  G+ T+    + 
Sbjct: 422 EIISIGSEVDYRRVAAKNSAENVHYLWPLNDAVLEEFEKMWRQVTDQYGGEITSA--TLP 481

Query: 391 VVMGRILQVPSGANGCAYFPFEELCDKPLGAADYFGLFKKFHTLALEGIPIFGLHNRTAA 445
           V+ GR ++VP   +G A F FE LC +P+GAADY  + K +HT+ +  IP   +  R  A
Sbjct: 482 VMFGRTVEVPESCSGVARFTFEYLCGRPVGAADYIAVAKNYHTIFISDIPAMSMEIRDKA 541

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8WV936.5e-6939.08AFG1-like ATPase OS=Homo sapiens OX=9606 GN=AFG1L PE=1 SV=2[more]
Q32PX95.5e-6840.05AFG1-like ATPase OS=Rattus norvegicus OX=10116 GN=Afg1l PE=2 SV=1[more]
Q3V3849.4e-6840.29AFG1-like ATPase OS=Mus musculus OX=10090 GN=Afg1l PE=1 SV=1[more]
P464413.6e-5934.10Putative ATPase N2B OS=Haematobia irritans OX=7368 PE=2 SV=1[more]
Q5TYS01.3e-5335.47Lactation elevated protein 1 homolog B OS=Danio rerio OX=7955 GN=lace1b PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A5A7SYX67.5e-27895.60Lactation elevated protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S4DV191.6e-27595.00lactation elevated protein 1 OS=Cucumis melo OX=3656 GN=LOC103487322 PE=3 SV=1[more]
A0A6J1JJY17.3e-27393.19AFG1-like ATPase OS=Cucurbita maxima OX=3661 GN=LOC111485772 PE=3 SV=1[more]
A0A0A0LTY33.1e-27194.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G277450 PE=3 SV=1[more]
A0A6J1C0D01.2e-27093.79AFG1-like ATPase OS=Momordica charantia OX=3673 GN=LOC111007319 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038880754.16.7e-28196.20AFG1-like ATPase isoform X4 [Benincasa hispida][more]
XP_038880753.11.7e-27996.01AFG1-like ATPase isoform X3 [Benincasa hispida][more]
XP_038880752.12.2e-27995.82AFG1-like ATPase isoform X2 [Benincasa hispida][more]
XP_038880751.15.3e-27895.63AFG1-like ATPase isoform X1 [Benincasa hispida][more]
KAA0035116.11.6e-27795.60lactation elevated protein 1 [Cucumis melo var. makuwa] >TYJ95963.1 lactation el... [more]
Match NameE-valueIdentityDescription
AT4G28070.21.3e-20081.10AFG1-like ATPase family protein [more]
AT4G30490.11.6e-20073.89AFG1-like ATPase family protein [more]
AT4G28070.14.8e-19278.71AFG1-like ATPase family protein [more]
AT2G25530.19.7e-4432.58AFG1-like ATPase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 103..123
NoneNo IPR availablePANTHERPTHR12169:SF24AFG1-LIKE ATPASE FAMILY PROTEIN-RELATEDcoord: 43..487
IPR005654ATPase, AFG1-likePFAMPF03969AFG1_ATPasecoord: 79..424
e-value: 3.6E-88
score: 295.9
IPR005654ATPase, AFG1-likePANTHERPTHR12169ATPASE N2Bcoord: 43..487
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 113..269
e-value: 1.4E-20
score: 76.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 147..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G010900.1Lsi06G010900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity