Lsi06G005970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G005970
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionHeat stress transcription factor B-4b-like
Locationchr06: 8182173 .. 8184007 (-)
RNA-Seq ExpressionLsi06G005970
SyntenyLsi06G005970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGTGAGTGTGTAATAGAAGAAAGAAGAAGGTGTGAGGGAAGAAAAATGGAAGAAAAAATGGAGGAGATGATTGAGAATTATAATGTGGGATTTTGTTCAGTTGAGTCTCAGAAGGCAAATAATCCGGCGCCGTTTTTGACAAAAACTTATGATTTGGTGGAGGATCCTACCACTGATCACATTGTTTCTTGGGGCCAATCTCATTCAACCTTTGTTGTTTGGAGACCCTCTGAGTTTGCTACTCATATTCTTCCCAACTACTTCAAACACAACAATTTCTCAAGCTTTGTTAGACAACTCAACACTTATGTATGTATACTCTCTCTCTCTCTCTCTCTCGTTTTCGTTTTTCGTTTTCTTCGATCATTCATCTTTGTCGTTACTCAACGTCTATGACGTTATAAGATAGAAATAATCTCTCGATGGAGTTATATAATGCGTCAGAAGATATATGTTCTGTCGACAATCGTATTCCCGACTCTGTTTTCAATCGTCGGGATATACTCTCTCTTGTATTGTTAGTATAATGTAAGGAGAGAATTTGAACTTATAGGTCGTCTAATAAGATAGTAGAAACTGCTCGAGCTAGCTATGATGTTGTCAGTGGATGATTCTTTAACCTACGAGCTATAAGAGACTACGTATTTTTTTTTTTTAAAAAAAATAATTGGGTTAAGATGGAGATTGAACCTATGACTTGTAAGTAAAGGGAAAAAGATACTTTAGTTCGGTTTTCTCTAATAAAGATCGGCGTTAATTAGTGCTAACTATCATTTGAACGATGCTTAGGTAATTAGCTTCATACCTTGATGAGATTTTTTAAATTAAACCTAGCTGCCTATGAAACAAAACACTTGTGTGCTTAAGCACCACTTGTGCCCTCATGCTAATCATGATTCTTTTTGTGAGATTCAACCCCTACTACCTATATGAAAAAGTATACTAAAATCTAATCACTTTTGAGCTATACTCAATTTGGTTAGCATTCTCTCGTTAAATTTGAAGAATAATTTAAAACTAACAATTCACGAGTAGAAATTATCAAATTATCAATGTTGTAATATATGTCTAATCTTAATTAGTTTTACATATTACAATGTGTATAAGAAAATATTGTCTAGATAACTACTAATTGAACTATGTTCGGATTTGTTCTCTCTCTCTTGTAGGGATTCAAGAAAATAGTGGCAGAGAGATGGGAATTTGGGAATGAAAACTTCAAGAAAGGAGAGAAGCAATTACTATTAGAGATTCAAAGAAGAAAATCTCATAATCACAACAACAATAATCAACAAATCCCATTCCAATTCTTCCATCTACACCAACACCAACACCAACTCAGCCTCTGTTACACCACCACTTCCTCCTCTGATCCTGACATCCTGGCGGCGCTAATGCAAGACAACCGTCGTCTACGACGGAGAAACTTCATGCTACTGTCAGAGCTGGCTCAGATGAAGAATCTCTACTCCGACATCATCTACTTCATCCAAAACAACGTTAAACCACTTGATCACAAATATGGAAGCAGATCGATTCCAAAGTTGGTTGAGTTGGACCCTCCGTCACCCGAGATCAGAGTAGAAGAGGGAAATGAGATGGTGAAGCTTTTTGGGGTTCCAATTAGAGGCAAGAAGAGAGTGCATCCCGAAGAGAGTGAGGTTCAAATGATGAGCAACAATGGTGGATATGATTTACACTAATTTTATATGTTTTGTTGTAATGTTATTGAGATTTTTGTACATAGATATTGAGGGAGTCACCATGGTATATTTTGATTGGGAATGGTGATCATGATTCTTCAAAATAAAATAAAGAAGGTTTAATTTTATT

mRNA sequence

GTTGTGAGTGTGTAATAGAAGAAAGAAGAAGGTGTGAGGGAAGAAAAATGGAAGAAAAAATGGAGGAGATGATTGAGAATTATAATGTGGGATTTTGTTCAGTTGAGTCTCAGAAGGCAAATAATCCGGCGCCGTTTTTGACAAAAACTTATGATTTGGTGGAGGATCCTACCACTGATCACATTGTTTCTTGGGGCCAATCTCATTCAACCTTTGTTGTTTGGAGACCCTCTGAGTTTGCTACTCATATTCTTCCCAACTACTTCAAACACAACAATTTCTCAAGCTTTGTTAGACAACTCAACACTTATGGATTCAAGAAAATAGTGGCAGAGAGATGGGAATTTGGGAATGAAAACTTCAAGAAAGGAGAGAAGCAATTACTATTAGAGATTCAAAGAAGAAAATCTCATAATCACAACAACAATAATCAACAAATCCCATTCCAATTCTTCCATCTACACCAACACCAACACCAACTCAGCCTCTGTTACACCACCACTTCCTCCTCTGATCCTGACATCCTGGCGGCGCTAATGCAAGACAACCGTCGTCTACGACGGAGAAACTTCATGCTACTGTCAGAGCTGGCTCAGATGAAGAATCTCTACTCCGACATCATCTACTTCATCCAAAACAACGTTAAACCACTTGATCACAAATATGGAAGCAGATCGATTCCAAAGTTGGTTGAGTTGGACCCTCCGTCACCCGAGATCAGAGTAGAAGAGGGAAATGAGATGGTGAAGCTTTTTGGGGTTCCAATTAGAGGCAAGAAGAGAGTGCATCCCGAAGAGAGTGAGGTTCAAATGATGAGCAACAATGGTGGATATGATTTACACTAATTTTATATGTTTTGTTGTAATGTTATTGAGATTTTTGTACATAGATATTGAGGGAGTCACCATGGTATATTTTGATTGGGAATGGTGATCATGATTCTTCAAAATAAAATAAAGAAGGTTTAATTTTATT

Coding sequence (CDS)

ATGGAAGAAAAAATGGAGGAGATGATTGAGAATTATAATGTGGGATTTTGTTCAGTTGAGTCTCAGAAGGCAAATAATCCGGCGCCGTTTTTGACAAAAACTTATGATTTGGTGGAGGATCCTACCACTGATCACATTGTTTCTTGGGGCCAATCTCATTCAACCTTTGTTGTTTGGAGACCCTCTGAGTTTGCTACTCATATTCTTCCCAACTACTTCAAACACAACAATTTCTCAAGCTTTGTTAGACAACTCAACACTTATGGATTCAAGAAAATAGTGGCAGAGAGATGGGAATTTGGGAATGAAAACTTCAAGAAAGGAGAGAAGCAATTACTATTAGAGATTCAAAGAAGAAAATCTCATAATCACAACAACAATAATCAACAAATCCCATTCCAATTCTTCCATCTACACCAACACCAACACCAACTCAGCCTCTGTTACACCACCACTTCCTCCTCTGATCCTGACATCCTGGCGGCGCTAATGCAAGACAACCGTCGTCTACGACGGAGAAACTTCATGCTACTGTCAGAGCTGGCTCAGATGAAGAATCTCTACTCCGACATCATCTACTTCATCCAAAACAACGTTAAACCACTTGATCACAAATATGGAAGCAGATCGATTCCAAAGTTGGTTGAGTTGGACCCTCCGTCACCCGAGATCAGAGTAGAAGAGGGAAATGAGATGGTGAAGCTTTTTGGGGTTCCAATTAGAGGCAAGAAGAGAGTGCATCCCGAAGAGAGTGAGGTTCAAATGATGAGCAACAATGGTGGATATGATTTACACTAA

Protein sequence

MEEKMEEMIENYNVGFCSVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSHNHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLLSELAQMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPPSPEIRVEEGNEMVKLFGVPIRGKKRVHPEESEVQMMSNNGGYDLH
Homology
BLAST of Lsi06G005970 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.0e-60
Identity = 136/270 (50.37%), Postives = 168/270 (62.22%), Query Frame = 0

Query: 27  PAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEFATHILPNYFKHNNFSSFVRQLN 86
           PAPFLTKTY LV+DP TDHIVSWG+  +TFVVWRP EFA  +LPNYFKHNNFSSFVRQLN
Sbjct: 32  PAPFLTKTYQLVDDPCTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLN 91

Query: 87  TYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKS--------------HNHNNNNQQI- 146
           TYGF+KIVA+RWEF NE F+KG K LL EI RRKS              H+H+ N   + 
Sbjct: 92  TYGFRKIVADRWEFANEFFRKGAKHLLAEIHRRKSSQPPPPPMPHQPYHHHHHLNPFSLP 151

Query: 147 --PFQFFHLHQHQHQLSLCYTTTSSSD----PDILAALMQDNRRLRRRNFMLLSELAQMK 206
             P  + H H  Q + +     T + D     D LAAL +DNR+LRRRN +LLSELA MK
Sbjct: 152 PPPPAYHHHHLIQEEPATTAHCTVAGDGGEGGDFLAALSEDNRQLRRRNSLLLSELAHMK 211

Query: 207 NLYSDIIYFIQNNVKPL---------------DHKYGSRSIPKLVELD-------PPSPE 253
            LY+DIIYF+QN+V P+                H   + +  +L+ELD       PP P+
Sbjct: 212 KLYNDIIYFLQNHVAPVTTTTTTPSSTAMAAAQHHLPAAASCRLMELDSPDHSPPPPPPK 271

BLAST of Lsi06G005970 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 9.8e-56
Identity = 130/259 (50.19%), Postives = 164/259 (63.32%), Query Frame = 0

Query: 27  PAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEFATHILPNYFKHNNFSSFVRQLN 86
           PAPFL+KTY LV+DP+TD +VSWG+  +TFVVWRP EFA  +LPNYFKHNNFSSFVRQLN
Sbjct: 34  PAPFLSKTYQLVDDPSTDDVVSWGEDEATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLN 93

Query: 87  TYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSHNHNNNNQQIPFQFFHLHQHQHQLS 146
           TYGF+KIVA+RWEF NE F+KG K LL EI RRKS   ++ +Q  P   F +HQH + LS
Sbjct: 94  TYGFRKIVADRWEFANEFFRKGAKHLLSEIHRRKS---SSCSQPQPPPPFPMHQH-YPLS 153

Query: 147 LCYTTTSSSDP----------------------------DILAALMQDNRRLRRRNFMLL 206
           L    T+   P                            D+LAAL +DNR+LRRRN +LL
Sbjct: 154 LFSPPTTPRSPPVGAAAAAAYHFQEEYCSSPADYAGGGGDLLAALSEDNRQLRRRNSLLL 213

Query: 207 SELAQMKNLYSDIIYFIQNNVKPL-DHKYGSRSIPKLVELDPPSPEIR--------VEEG 246
           SELA M+ LY+DIIYF+QN+V+P+      + +  +LVEL P + E R          + 
Sbjct: 214 SELAHMRKLYNDIIYFLQNHVEPVAPPPLAAATSCRLVELGPSTTERRRCAASPSGDNDD 273

BLAST of Lsi06G005970 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.9e-51
Identity = 124/258 (48.06%), Postives = 153/258 (59.30%), Query Frame = 0

Query: 5   MEEMIENYNVGF---------CSVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHST 64
           M  M+EN   G+           VE Q    PAPFLTKTY LV+DP TDH+VSWG   +T
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 65  FVVWRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLE 124
           FVVWRP EFA  +LPNYFKHNNFSSFVRQLNTYGF+KIV +RWEF NE FK+GEK LL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 125 IQRRKSH-----------NHNNNNQQIPF---QFFHLHQHQ----HQLSLCYTTTSSSDP 184
           I RRK+            +H++   QIPF    FF L   +     +       +  S P
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTPEEDHYWCDDSPPSRP 180

Query: 185 DIL----------AALMQDNRRLRRRNFMLLSELAQMKNLYSDIIYFIQNNVKPL----D 222
            ++           AL +DN RLRR N +L+SELA MK LY+DIIYF+QN+VKP+    +
Sbjct: 181 RVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAPSNN 240

BLAST of Lsi06G005970 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.8e-47
Identity = 111/230 (48.26%), Postives = 133/230 (57.83%), Query Frame = 0

Query: 27  PAPFLTKTYDLVEDPTTDHIVSWGQSH-STFVVWRPSEFATHILPNYFKHNNFSSFVRQL 86
           PAPFLTKTY LV+DP TDHIVSWG    STFVVWRP EFA  ILPNYFKHNNFSSFVRQL
Sbjct: 22  PAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILPNYFKHNNFSSFVRQL 81

Query: 87  NTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSHNHNNNNQQIP-------FQFFH- 146
           NTYGF+K+V ERWEF NE F+KGEKQLL EI RRK+ + +  +   P       F  FH 
Sbjct: 82  NTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTASPSPPPFFAPPHFPLFHH 141

Query: 147 ------LHQH----------QHQLSLCYTTTSSSDPDI---------------------- 203
                  H H           H + + +      +P++                      
Sbjct: 142 PGVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATRLLALGGPAPSPSSAEAG 201

BLAST of Lsi06G005970 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 6.6e-44
Identity = 112/226 (49.56%), Postives = 129/226 (57.08%), Query Frame = 0

Query: 27  PAPFLTKTYDLVEDPTTDHIVSW-----GQSHSTFVVWRPSEFATHILPNYFKHNNFSSF 86
           PAPFLTKTY LV+DP TDH+VSW     G+S S+FVVWRP EFA  ILPNYFKH+NFSSF
Sbjct: 23  PAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHSNFSSF 82

Query: 87  VRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSHNHNNNNQQIPFQFFHLHQH 146
           VRQLNTYGF+K+V ERWEF NE F+KGEKQLL EI RRKS          P   F   +H
Sbjct: 83  VRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPFFAPRH 142

Query: 147 --------------QHQLSLCYTTT--------------------SSSDPDIL------- 203
                           +L    TTT                    S   P I        
Sbjct: 143 FAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLSQLGPVIAPARRAAA 202

BLAST of Lsi06G005970 vs. ExPASy TrEMBL
Match: A0A0A0M2Y7 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G629180 PE=3 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.3e-122
Identity = 231/261 (88.51%), Postives = 240/261 (91.95%), Query Frame = 0

Query: 1   MEEKMEEMIENYNVGFC--SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVV 60
           MEE+ME   ENYN G C  SVESQKANNPAPFL+KTYDLVEDPTTDHIVSWGQS +TF+V
Sbjct: 1   MEERME---ENYNAGLCSSSVESQKANNPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIV 60

Query: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120
           WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR
Sbjct: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120

Query: 121 RKSHNHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLL 180
           RKSHNHNNN+QQIPFQ FHLHQ Q QLSLCYTTTSSSDPDILAAL QDNRRLRRRNFMLL
Sbjct: 121 RKSHNHNNNSQQIPFQLFHLHQ-QQQLSLCYTTTSSSDPDILAALTQDNRRLRRRNFMLL 180

Query: 181 SELAQMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPP------SPEIRVEEGNEM 240
           SELAQMKNLYSDIIYFIQNNVKPLD+KY +RS+PKLVELDPP      SPEIR+EEGN M
Sbjct: 181 SELAQMKNLYSDIIYFIQNNVKPLDNKYCNRSVPKLVELDPPSSPTPMSPEIRLEEGNGM 240

Query: 241 VKLFGVPIRGKKRVHPEESEV 254
           VKLFGVPIRGKKRV PEESEV
Sbjct: 241 VKLFGVPIRGKKRVLPEESEV 257

BLAST of Lsi06G005970 vs. ExPASy TrEMBL
Match: A0A5D3DXU1 (Heat stress transcription factor B-4b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G00970 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.1e-122
Identity = 231/261 (88.51%), Postives = 240/261 (91.95%), Query Frame = 0

Query: 1   MEEKMEEMIENYNVGFC--SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVV 60
           MEEKME   ENYNVG C  SVESQKANNPAPFL+KTYDLVEDPTTDHIVSWGQS +TF+V
Sbjct: 1   MEEKME---ENYNVGLCSSSVESQKANNPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIV 60

Query: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120
           WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR
Sbjct: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120

Query: 121 RKSHNHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLL 180
           RKSHNHNNNNQQIPFQFFHLHQ Q QLSLCYTTTSS+DPDIL AL QDNRRLRRRNFMLL
Sbjct: 121 RKSHNHNNNNQQIPFQFFHLHQ-QQQLSLCYTTTSSADPDILTALTQDNRRLRRRNFMLL 180

Query: 181 SELAQMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPP------SPEIRVEEGNEM 240
           SELAQMKNLYSDIIYFIQNNVKPLD+KY +RS+PKLVELD P      SPEIR+EEG+ M
Sbjct: 181 SELAQMKNLYSDIIYFIQNNVKPLDNKYCNRSVPKLVELDSPSSPMPMSPEIRLEEGSGM 240

Query: 241 VKLFGVPIRGKKRVHPEESEV 254
           VKLFGVPIRGKKRV PEESEV
Sbjct: 241 VKLFGVPIRGKKRVLPEESEV 257

BLAST of Lsi06G005970 vs. ExPASy TrEMBL
Match: A0A5A7ULL5 (Heat stress transcription factor B-4b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold319G001010 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 3.7e-122
Identity = 227/257 (88.33%), Postives = 238/257 (92.61%), Query Frame = 0

Query: 5   MEEMIENYNVGFC--SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPS 64
           MEE ++NYNVG C  SVESQKANNPAPFL+KTYDLVEDPTTDHIVSWGQS +TF+VWRPS
Sbjct: 1   MEEKMDNYNVGLCSSSVESQKANNPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIVWRPS 60

Query: 65  EFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSH 124
           EFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSH
Sbjct: 61  EFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSH 120

Query: 125 NHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLLSELA 184
           NHNNNNQQIPFQFFHLHQ Q QLSLCYTTTSS+DPDIL AL QDNRRLRRRNFMLLSELA
Sbjct: 121 NHNNNNQQIPFQFFHLHQ-QQQLSLCYTTTSSADPDILTALTQDNRRLRRRNFMLLSELA 180

Query: 185 QMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPP------SPEIRVEEGNEMVKLF 244
           QMKNLYSDIIYFIQNNVKPLD+KY +RS+PKLVELD P      SPEIR+EEG+ MVKLF
Sbjct: 181 QMKNLYSDIIYFIQNNVKPLDNKYCNRSVPKLVELDSPSSPMPMSPEIRLEEGSGMVKLF 240

Query: 245 GVPIRGKKRVHPEESEV 254
           GVPIRGKKRV PEESEV
Sbjct: 241 GVPIRGKKRVLPEESEV 256

BLAST of Lsi06G005970 vs. ExPASy TrEMBL
Match: A0A1S3BPZ9 (heat stress transcription factor B-4b-like OS=Cucumis melo OX=3656 GN=LOC103491968 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.8e-122
Identity = 230/261 (88.12%), Postives = 240/261 (91.95%), Query Frame = 0

Query: 1   MEEKMEEMIENYNVGFC--SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVV 60
           MEEKME   +NYNVG C  SVESQKANNPAPFL+KTYDLVEDPTTDHIVSWGQS +TF+V
Sbjct: 1   MEEKME---DNYNVGLCSSSVESQKANNPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIV 60

Query: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120
           WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR
Sbjct: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120

Query: 121 RKSHNHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLL 180
           RKSHNHNNNNQQIPFQFFHLHQ Q QLSLCYTTTSS+DPDIL AL QDNRRLRRRNFMLL
Sbjct: 121 RKSHNHNNNNQQIPFQFFHLHQ-QQQLSLCYTTTSSADPDILTALTQDNRRLRRRNFMLL 180

Query: 181 SELAQMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPP------SPEIRVEEGNEM 240
           SELAQMKNLYSDIIYFIQNNVKPLD+KY +RS+PKLVELD P      SPEIR+EEG+ M
Sbjct: 181 SELAQMKNLYSDIIYFIQNNVKPLDNKYCNRSVPKLVELDSPSSPMPMSPEIRLEEGSGM 240

Query: 241 VKLFGVPIRGKKRVHPEESEV 254
           VKLFGVPIRGKKRV PEESEV
Sbjct: 241 VKLFGVPIRGKKRVLPEESEV 257

BLAST of Lsi06G005970 vs. ExPASy TrEMBL
Match: A0A6J1JGJ8 (heat stress transcription factor B-4b-like OS=Cucurbita maxima OX=3661 GN=LOC111485507 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.6e-104
Identity = 203/265 (76.60%), Postives = 222/265 (83.77%), Query Frame = 0

Query: 5   MEEMIENYNVGFCSVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEF 64
           MEEMIENY+VGF S ESQKA NPAPFL KTY+LVED  TDHIVSWG+SH+TFVVWRP EF
Sbjct: 1   MEEMIENYSVGFWSAESQKA-NPAPFLAKTYELVEDAATDHIVSWGRSHTTFVVWRPPEF 60

Query: 65  ATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSHNH 124
           ATHILPNYFKHNNFSSFVRQLN+YGFKKIVA+RWEFGN+NFKKGEKQLLLEIQRRK HNH
Sbjct: 61  ATHILPNYFKHNNFSSFVRQLNSYGFKKIVAKRWEFGNQNFKKGEKQLLLEIQRRKPHNH 120

Query: 125 NNN---NQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLLSEL 184
           NNN    QQIPFQF +        +   T++SSSDPDILAAL QDNRRLRRRN+MLLSEL
Sbjct: 121 NNNLHQQQQIPFQFCY-------TAAAATSSSSSDPDILAALTQDNRRLRRRNYMLLSEL 180

Query: 185 AQMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPPSP------EIRVEEGNEMVKL 244
            QMKNLYSDIIYFIQN+VKPLDHKYG RS+PKLV+L PP P      E  VEEG+  V+L
Sbjct: 181 TQMKNLYSDIIYFIQNHVKPLDHKYG-RSVPKLVDLKPPPPPPPPQTEAGVEEGSGSVQL 240

Query: 245 FGVPIRGKKRVHPEESEVQMMSNNG 261
           FGVPIRGKKRVH EE E+QM S+ G
Sbjct: 241 FGVPIRGKKRVHHEEGELQMSSHGG 256

BLAST of Lsi06G005970 vs. NCBI nr
Match: XP_038877193.1 (heat stress transcription factor B-4b-like [Benincasa hispida])

HSP 1 Score: 453.8 bits (1166), Expect = 1.1e-123
Identity = 228/253 (90.12%), Postives = 237/253 (93.68%), Query Frame = 0

Query: 5   MEEMIENYNVGFCSVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEF 64
           ME +IENYNVGFCSVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEF
Sbjct: 1   MEGIIENYNVGFCSVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEF 60

Query: 65  ATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSHNH 124
           ATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGE+QLLLEIQRRKSHNH
Sbjct: 61  ATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGERQLLLEIQRRKSHNH 120

Query: 125 NNNNQQIPFQFFHLH-QHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLLSELAQ 184
            NNNQQIPFQFFHLH QHQ QLSLCYTTTSSSDPDILAAL QDNRRLRRRNFMLLSELA 
Sbjct: 121 -NNNQQIPFQFFHLHQQHQQQLSLCYTTTSSSDPDILAALTQDNRRLRRRNFMLLSELAH 180

Query: 185 MKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPPS------PEIRVEEGNEMVKLFG 244
           MKNLYS+IIYFIQNNVKPLDHKYGS+S+PKL+EL+P S      PE+R+EEGN MVKLFG
Sbjct: 181 MKNLYSNIIYFIQNNVKPLDHKYGSKSVPKLIELNPLSLPQPALPEVRLEEGNGMVKLFG 240

Query: 245 VPIRGKKRVHPEE 251
           VPIRGKKR H EE
Sbjct: 241 VPIRGKKRGHREE 252

BLAST of Lsi06G005970 vs. NCBI nr
Match: XP_004139066.1 (heat stress transcription factor B-4b [Cucumis sativus] >KGN66561.1 hypothetical protein Csa_007203 [Cucumis sativus])

HSP 1 Score: 449.1 bits (1154), Expect = 2.6e-122
Identity = 231/261 (88.51%), Postives = 240/261 (91.95%), Query Frame = 0

Query: 1   MEEKMEEMIENYNVGFC--SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVV 60
           MEE+ME   ENYN G C  SVESQKANNPAPFL+KTYDLVEDPTTDHIVSWGQS +TF+V
Sbjct: 1   MEERME---ENYNAGLCSSSVESQKANNPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIV 60

Query: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120
           WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR
Sbjct: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120

Query: 121 RKSHNHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLL 180
           RKSHNHNNN+QQIPFQ FHLHQ Q QLSLCYTTTSSSDPDILAAL QDNRRLRRRNFMLL
Sbjct: 121 RKSHNHNNNSQQIPFQLFHLHQ-QQQLSLCYTTTSSSDPDILAALTQDNRRLRRRNFMLL 180

Query: 181 SELAQMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPP------SPEIRVEEGNEM 240
           SELAQMKNLYSDIIYFIQNNVKPLD+KY +RS+PKLVELDPP      SPEIR+EEGN M
Sbjct: 181 SELAQMKNLYSDIIYFIQNNVKPLDNKYCNRSVPKLVELDPPSSPTPMSPEIRLEEGNGM 240

Query: 241 VKLFGVPIRGKKRVHPEESEV 254
           VKLFGVPIRGKKRV PEESEV
Sbjct: 241 VKLFGVPIRGKKRVLPEESEV 257

BLAST of Lsi06G005970 vs. NCBI nr
Match: TYK28179.1 (heat stress transcription factor B-4b-like [Cucumis melo var. makuwa])

HSP 1 Score: 448.4 bits (1152), Expect = 4.4e-122
Identity = 231/261 (88.51%), Postives = 240/261 (91.95%), Query Frame = 0

Query: 1   MEEKMEEMIENYNVGFC--SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVV 60
           MEEKME   ENYNVG C  SVESQKANNPAPFL+KTYDLVEDPTTDHIVSWGQS +TF+V
Sbjct: 1   MEEKME---ENYNVGLCSSSVESQKANNPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIV 60

Query: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120
           WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR
Sbjct: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120

Query: 121 RKSHNHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLL 180
           RKSHNHNNNNQQIPFQFFHLHQ Q QLSLCYTTTSS+DPDIL AL QDNRRLRRRNFMLL
Sbjct: 121 RKSHNHNNNNQQIPFQFFHLHQ-QQQLSLCYTTTSSADPDILTALTQDNRRLRRRNFMLL 180

Query: 181 SELAQMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPP------SPEIRVEEGNEM 240
           SELAQMKNLYSDIIYFIQNNVKPLD+KY +RS+PKLVELD P      SPEIR+EEG+ M
Sbjct: 181 SELAQMKNLYSDIIYFIQNNVKPLDNKYCNRSVPKLVELDSPSSPMPMSPEIRLEEGSGM 240

Query: 241 VKLFGVPIRGKKRVHPEESEV 254
           VKLFGVPIRGKKRV PEESEV
Sbjct: 241 VKLFGVPIRGKKRVLPEESEV 257

BLAST of Lsi06G005970 vs. NCBI nr
Match: KAA0055968.1 (heat stress transcription factor B-4b-like [Cucumis melo var. makuwa])

HSP 1 Score: 447.6 bits (1150), Expect = 7.5e-122
Identity = 227/257 (88.33%), Postives = 238/257 (92.61%), Query Frame = 0

Query: 5   MEEMIENYNVGFC--SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPS 64
           MEE ++NYNVG C  SVESQKANNPAPFL+KTYDLVEDPTTDHIVSWGQS +TF+VWRPS
Sbjct: 1   MEEKMDNYNVGLCSSSVESQKANNPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIVWRPS 60

Query: 65  EFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSH 124
           EFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSH
Sbjct: 61  EFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSH 120

Query: 125 NHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLLSELA 184
           NHNNNNQQIPFQFFHLHQ Q QLSLCYTTTSS+DPDIL AL QDNRRLRRRNFMLLSELA
Sbjct: 121 NHNNNNQQIPFQFFHLHQ-QQQLSLCYTTTSSADPDILTALTQDNRRLRRRNFMLLSELA 180

Query: 185 QMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPP------SPEIRVEEGNEMVKLF 244
           QMKNLYSDIIYFIQNNVKPLD+KY +RS+PKLVELD P      SPEIR+EEG+ MVKLF
Sbjct: 181 QMKNLYSDIIYFIQNNVKPLDNKYCNRSVPKLVELDSPSSPMPMSPEIRLEEGSGMVKLF 240

Query: 245 GVPIRGKKRVHPEESEV 254
           GVPIRGKKRV PEESEV
Sbjct: 241 GVPIRGKKRVLPEESEV 256

BLAST of Lsi06G005970 vs. NCBI nr
Match: XP_008450326.1 (PREDICTED: heat stress transcription factor B-4b-like [Cucumis melo])

HSP 1 Score: 447.2 bits (1149), Expect = 9.9e-122
Identity = 230/261 (88.12%), Postives = 240/261 (91.95%), Query Frame = 0

Query: 1   MEEKMEEMIENYNVGFC--SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVV 60
           MEEKME   +NYNVG C  SVESQKANNPAPFL+KTYDLVEDPTTDHIVSWGQS +TF+V
Sbjct: 1   MEEKME---DNYNVGLCSSSVESQKANNPAPFLSKTYDLVEDPTTDHIVSWGQSLTTFIV 60

Query: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120
           WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR
Sbjct: 61  WRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQR 120

Query: 121 RKSHNHNNNNQQIPFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLRRRNFMLL 180
           RKSHNHNNNNQQIPFQFFHLHQ Q QLSLCYTTTSS+DPDIL AL QDNRRLRRRNFMLL
Sbjct: 121 RKSHNHNNNNQQIPFQFFHLHQ-QQQLSLCYTTTSSADPDILTALTQDNRRLRRRNFMLL 180

Query: 181 SELAQMKNLYSDIIYFIQNNVKPLDHKYGSRSIPKLVELDPP------SPEIRVEEGNEM 240
           SELAQMKNLYSDIIYFIQNNVKPLD+KY +RS+PKLVELD P      SPEIR+EEG+ M
Sbjct: 181 SELAQMKNLYSDIIYFIQNNVKPLDNKYCNRSVPKLVELDSPSSPMPMSPEIRLEEGSGM 240

Query: 241 VKLFGVPIRGKKRVHPEESEV 254
           VKLFGVPIRGKKRV PEESEV
Sbjct: 241 VKLFGVPIRGKKRVLPEESEV 257

BLAST of Lsi06G005970 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 204.1 bits (518), Expect = 1.4e-52
Identity = 124/258 (48.06%), Postives = 153/258 (59.30%), Query Frame = 0

Query: 5   MEEMIENYNVGF---------CSVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHST 64
           M  M+EN   G+           VE Q    PAPFLTKTY LV+DP TDH+VSWG   +T
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 65  FVVWRPSEFATHILPNYFKHNNFSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLE 124
           FVVWRP EFA  +LPNYFKHNNFSSFVRQLNTYGF+KIV +RWEF NE FK+GEK LL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 125 IQRRKSH-----------NHNNNNQQIPF---QFFHLHQHQ----HQLSLCYTTTSSSDP 184
           I RRK+            +H++   QIPF    FF L   +     +       +  S P
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTPEEDHYWCDDSPPSRP 180

Query: 185 DIL----------AALMQDNRRLRRRNFMLLSELAQMKNLYSDIIYFIQNNVKPL----D 222
            ++           AL +DN RLRR N +L+SELA MK LY+DIIYF+QN+VKP+    +
Sbjct: 181 RVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAPSNN 240

BLAST of Lsi06G005970 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 173.7 bits (439), Expect = 2.0e-43
Identity = 108/269 (40.15%), Postives = 150/269 (55.76%), Query Frame = 0

Query: 21  SQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEFATHILPNYFKHNNFSS 80
           S + + P PFLTKT++LVED + D ++SW +  S+F+VW P++FA  +LP +FKHNNFSS
Sbjct: 15  SSQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSS 74

Query: 81  FVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRK-------------------- 140
           FVRQLNTYGFKK+V +RWEF N+ FK+GEK+LL EIQRRK                    
Sbjct: 75  FVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVVAPSSEQRNQTM 134

Query: 141 ------SHNHNNNNQQI---PFQFFHLHQHQHQLSLCYTTTSSSDPDILAALMQDNRRLR 200
                 S   NNNNQ +   P  ++           C+ T ++ +  +   L+++N +LR
Sbjct: 135 VVSPSNSGEDNNNNQVMSSSPSSWY-----------CHQTKTTGNGGLSVELLEENEKLR 194

Query: 201 RRNFMLLSELAQMKNLYSDIIYFIQNNV--KPLDHKY---GSRSIPKLVELDPPSPEIRV 255
            +N  L  EL QMK++  +I   + N V  +P D  Y   GS S P          E+ +
Sbjct: 195 SQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKRFSEMEI 254

BLAST of Lsi06G005970 vs. TAIR 10
Match: AT2G41690.1 (heat shock transcription factor B3 )

HSP 1 Score: 149.1 bits (375), Expect = 5.2e-36
Identity = 87/231 (37.66%), Postives = 133/231 (57.58%), Query Frame = 0

Query: 18  SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEFATHILPNYFKHNN 77
           S  + +   P PFL KTY +VEDPTTD ++SW +  + FVVW+P+EFA  +LP  FKH N
Sbjct: 29  STSTAELQPPPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCN 88

Query: 78  FSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSH--NHNNNNQQIPFQF 137
           FSSFVRQLNTYGF+K+   RWEF NE F+KG+++L+  I+RRKS   +HN +N Q+    
Sbjct: 89  FSSFVRQLNTYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTT 148

Query: 138 FHLHQHQHQ-LSLCY-----TTTSSSDPDILAALMQDNRRLRRRNFMLLSELAQMKNLYS 197
             ++Q  HQ + + +      ++++S   +  AL+ +N+ L+  N +L  EL + K    
Sbjct: 149 TMVNQEGHQRIGIDHHHEDQQSSATSSSFVYTALLDENKCLKNENELLSCELGKTKKKCK 208

Query: 198 DIIYFIQNNVKPLDHKYGSRSIPKLVELDPPSPEIRVEEGNEMVKLFGVPI 241
            ++  ++        +Y         E D        +E +E +KLFGV +
Sbjct: 209 QLMELVE--------RYRGEDEDATDESD--------DEEDEGLKLFGVKL 243

BLAST of Lsi06G005970 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 148.7 bits (374), Expect = 6.7e-36
Identity = 101/278 (36.33%), Postives = 141/278 (50.72%), Query Frame = 0

Query: 27  PAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEFATHILPNYFKHNNFSSFVRQLN 86
           P PFLTKTY LVEDP  D ++SW +  +TF+VWRP+EFA  +LP YFKHNNFSSFVRQLN
Sbjct: 57  PTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSSFVRQLN 116

Query: 87  TYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSHN----------------------- 146
           TYGF+K+V +RWEF N+ FK+GEK LL +IQRRK                          
Sbjct: 117 TYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAASAVTVAA 176

Query: 147 ----------HNNNNQQIPFQFFHLHQHQHQL-------SLCYTTTSSSDPDILAALMQD 206
                      N+  +Q+             +       SL  TT+ ++ P+    L+++
Sbjct: 177 VPVVAHIVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTTAPE----LVEE 236

Query: 207 NRRLRRRNFMLLSELAQMKNLYSDIIYFIQN-------------NVKPLDHKYGSRSIPK 251
           N RLR+ N  L  E+ ++K LY++I   + N               KPLD     + + +
Sbjct: 237 NERLRKDNERLRKEMTKLKGLYANIYTLMANFTPGQEDCAHLLPEGKPLDLLPERQEMSE 296

BLAST of Lsi06G005970 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 147.5 bits (371), Expect = 1.5e-35
Identity = 96/262 (36.64%), Postives = 150/262 (57.25%), Query Frame = 0

Query: 18  SVESQKANNPAPFLTKTYDLVEDPTTDHIVSWGQSHSTFVVWRPSEFATHILPNYFKHNN 77
           +V + + + PAPFL+KTY LV+D +TD +VSW +  + FVVW+ +EFA  +LP YFKHNN
Sbjct: 3   AVTAAQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNN 62

Query: 78  FSSFVRQLNTYGFKKIVAERWEFGNENFKKGEKQLLLEIQRRKSHNHNNNNQQI----PF 137
           FSSF+RQLNTYGF+K V ++WEF N+ F++G + LL +I+RRKS   +   + +    P 
Sbjct: 63  FSSFIRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPS 122

Query: 138 QFFHLHQHQHQLSLCYTTTSSSDP----DILAALMQDNRRLRRRNFMLLSELAQMKNLYS 197
           +        H  S   +  SS +P    +++A L  +N +L+R N  L SELA  K    
Sbjct: 123 ESNSGGGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLSSELAAAKKQRD 182

Query: 198 DIIYFIQNNVKPLDHKY-----GSRSIPKLVELDPPSP------EIRVEEG-NEMVKLFG 257
           +++ F+  ++K    +      G +  P  VE D  S           EEG  E +KLFG
Sbjct: 183 ELVTFLTGHLKVRPEQIDKMIKGGKFKP--VESDEESECEGCDGGGGAEEGVGEGLKLFG 242

Query: 258 VPIRGKKRVHPEESEVQMMSNN 260
           V ++G+++    + +  ++S +
Sbjct: 243 VWLKGERKKRDRDEKNYVVSGS 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XHZ01.0e-6050.37Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX89.8e-5650.19Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9C6351.9e-5148.06Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q67U942.8e-4748.26Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R86.6e-4449.56Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
A0A0A0M2Y71.3e-12288.51HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G629180 ... [more]
A0A5D3DXU12.1e-12288.51Heat stress transcription factor B-4b-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5A7ULL53.7e-12288.33Heat stress transcription factor B-4b-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BPZ94.8e-12288.12heat stress transcription factor B-4b-like OS=Cucumis melo OX=3656 GN=LOC1034919... [more]
A0A6J1JGJ82.6e-10476.60heat stress transcription factor B-4b-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038877193.11.1e-12390.12heat stress transcription factor B-4b-like [Benincasa hispida][more]
XP_004139066.12.6e-12288.51heat stress transcription factor B-4b [Cucumis sativus] >KGN66561.1 hypothetical... [more]
TYK28179.14.4e-12288.51heat stress transcription factor B-4b-like [Cucumis melo var. makuwa][more]
KAA0055968.17.5e-12288.33heat stress transcription factor B-4b-like [Cucumis melo var. makuwa][more]
XP_008450326.19.9e-12288.12PREDICTED: heat stress transcription factor B-4b-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G46264.11.4e-5248.06heat shock transcription factor B4 [more]
AT5G62020.12.0e-4340.15heat shock transcription factor B2A [more]
AT2G41690.15.2e-3637.66heat shock transcription factor B3 [more]
AT4G11660.16.7e-3636.33winged-helix DNA-binding transcription factor family protein [more]
AT4G36990.11.5e-3536.64heat shock factor 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 68..80
score: 75.6
coord: 30..53
score: 46.07
coord: 81..93
score: 79.12
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 26..119
e-value: 1.0E-52
score: 191.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 30..119
e-value: 5.6E-32
score: 110.2
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 69..93
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 21..121
e-value: 2.4E-36
score: 126.4
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 22..249
NoneNo IPR availablePANTHERPTHR10015:SF304HEAT STRESS TRANSCRIPTION FACTOR B-4Bcoord: 22..249
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 25..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G005970.1Lsi06G005970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding