Lsi05G022300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G022300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionethylene-responsive transcription factor 13-like
Locationchr05: 28858778 .. 28860206 (+)
RNA-Seq ExpressionLsi05G022300
SyntenyLsi05G022300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGCGTCCTTTTGAATGGGACTTATCAGTCAATAGATAATTACAAATCACCTCGCTCTGAAGACAGATATCTGCTCTGACTGTCAACGTAAGACCCATAAATTAGTTCTGAGGTTTCTGAGGAGACTGACAAACCGCCGGATATAGCTTTCTGTAATTGTTTATCAATGGTCACCTTGATCTTATATATTTCAATTCTATTTGGGGTATTTGACAACTCAGCATTTTCTTTTTCCTCCAGCCTTTTGACGTGATCGTTGAAGTTAATAGAATGGACAAGAGGTTTTAATCAAACGCCAAAATTACTATTGTATTTGCGGAAAACTTTGATGACCTTTTCTATTGCAAATGGCTCCAATTTAGCGGTAAGTTTTCAACATTTATATATTATATAAGAAGTAACCAAGAACATTCAAGTGAGAACTGTCCTTTATTAACAAATACTTGAAGCGCACGTTTTCAATGTTATCTAGCAAGAGTGCTGTGTGATGCTCCTTTTTAAATATTGCTACTCCAATTTATTTAAGAATTATTTTGGAATTTAAAATAAGGACAATATCATATGACATAAATTCGTAGTGGCAGGCAGATTATAAGAAAATGAAAGAAGTACAGTCAAACTATTCAATTTTTTATTCTAAATAGACATTTTTACATATGTGTATATATATATTTCATATATCACTGGTACCTAATATTTATGTGAACACGTGGTGTGGTTTGGAAGATTCAAAGAAAGTGGTGGTTGCGTTGTATTACTATTATATATAAAAGGGGTAGAGATGATGGGGATACAGAGTGTGGTTCCAGTAAGCTAAGGCAGAAGAGAATTAGAAGCAGAAAAATGTGGAGTAACAGCAACAGCAGTAGCTTGGAAGGGTCGTCGGATTTGTTTGTGTTGGAGCGGATTCGGCGGCATCTTCTGGAAGACGAATTAGGGAATAATGTTGATGAGGAGGGAAGGTGGGAGTGGGGGAATAATAGTACTGGAGGAGGTTCGAAGAGTAATGATAATAATAATAATATGGTCATGGTAGAACTGGGCGAAGAGATGAGAGGGAAGTCAGGGAAGGGATCAAGTATTATATGGTCGTCAGATGATCAGAGGGATCAAATCAGGGCGAGGGGTTTACAGTTCAGAGGAGTTAGGAGGAGGCCGTGGGGGAAGTACGCGGCAGAAATCAGAGACCCGAGCAGAAAAGGGGCTCGAATATGGCTTGGGACTTACCACACCGCCCAAGATGCGGCTTTAGCTTACGATCGAGCTGCTTTCCAGATGCGAGGGGCCAAAGCCAAGCTCAACTTCCCTCACATGATTGCTTCCACTTCCAACAACGACTACCCACGTCGTAGCTGTAAGCGTAGTTCGCCTAATTGTAATTCCCCCCGGACCCCTCCGCCCCTATTCAACTTCAACAACTACTGA

mRNA sequence

ATGTCTGCGGGTAGAGATGATGGGGATACAGAGTGTGGTTCCAGTAAGCTAAGGCAGAAGAGAATTAGAAGCAGAAAAATGTGGAGTAACAGCAACAGCAGTAGCTTGGAAGGGTCGTCGGATTTGTTTGTGTTGGAGCGGATTCGGCGGCATCTTCTGGAAGACGAATTAGGGAATAATGTTGATGAGGAGGGAAGGTGGGAGTGGGGGAATAATAGTACTGGAGGAGGTTCGAAGAGTAATGATAATAATAATAATATGGTCATGGTAGAACTGGGCGAAGAGATGAGAGGGAAGTCAGGGAAGGGATCAAGTATTATATGGTCGTCAGATGATCAGAGGGATCAAATCAGGGCGAGGGGTTTACAGTTCAGAGGAGTTAGGAGGAGGCCGTGGGGGAAGTACGCGGCAGAAATCAGAGACCCGAGCAGAAAAGGGGCTCGAATATGGCTTGGGACTTACCACACCGCCCAAGATGCGGCTTTAGCTTACGATCGAGCTGCTTTCCAGATGCGAGGGGCCAAAGCCAAGCTCAACTTCCCTCACATGATTGCTTCCACTTCCAACAACGACTACCCACGTCGTAGCTGTAAGCGTAGTTCGCCTAATTGTAATTCCCCCCGGACCCCTCCGCCCCTATTCAACTTCAACAACTACTGA

Coding sequence (CDS)

ATGTCTGCGGGTAGAGATGATGGGGATACAGAGTGTGGTTCCAGTAAGCTAAGGCAGAAGAGAATTAGAAGCAGAAAAATGTGGAGTAACAGCAACAGCAGTAGCTTGGAAGGGTCGTCGGATTTGTTTGTGTTGGAGCGGATTCGGCGGCATCTTCTGGAAGACGAATTAGGGAATAATGTTGATGAGGAGGGAAGGTGGGAGTGGGGGAATAATAGTACTGGAGGAGGTTCGAAGAGTAATGATAATAATAATAATATGGTCATGGTAGAACTGGGCGAAGAGATGAGAGGGAAGTCAGGGAAGGGATCAAGTATTATATGGTCGTCAGATGATCAGAGGGATCAAATCAGGGCGAGGGGTTTACAGTTCAGAGGAGTTAGGAGGAGGCCGTGGGGGAAGTACGCGGCAGAAATCAGAGACCCGAGCAGAAAAGGGGCTCGAATATGGCTTGGGACTTACCACACCGCCCAAGATGCGGCTTTAGCTTACGATCGAGCTGCTTTCCAGATGCGAGGGGCCAAAGCCAAGCTCAACTTCCCTCACATGATTGCTTCCACTTCCAACAACGACTACCCACGTCGTAGCTGTAAGCGTAGTTCGCCTAATTGTAATTCCCCCCGGACCCCTCCGCCCCTATTCAACTTCAACAACTACTGA

Protein sequence

MSAGRDDGDTECGSSKLRQKRIRSRKMWSNSNSSSLEGSSDLFVLERIRRHLLEDELGNNVDEEGRWEWGNNSTGGGSKSNDNNNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSPRTPPPLFNFNNY
Homology
BLAST of Lsi05G022300 vs. ExPASy Swiss-Prot
Match: Q8L9K1 (Ethylene-responsive transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=ERF13 PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 3.9e-26
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = 0

Query: 118 RARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAK 177
           R +G+Q+RGVRRRPWGK+AAEIRDP + GAR+WLGTY T +DAA+AYDRAAFQ+RG+KAK
Sbjct: 86  RQKGMQYRGVRRRPWGKFAAEIRDPKKNGARVWLGTYETPEDAAVAYDRAAFQLRGSKAK 145

Query: 178 LNFPHMIASTSNNDYPRRSCKRS 201
           LNFPH+I S        R  +RS
Sbjct: 146 LNFPHLIGSCKYEPVRIRPRRRS 168

BLAST of Lsi05G022300 vs. ExPASy Swiss-Prot
Match: Q40479 (Ethylene-responsive transcription factor 2 OS=Nicotiana tabacum OX=4097 GN=ERF2 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 6.9e-23
Identity = 51/83 (61.45%), Postives = 67/83 (80.72%), Query Frame = 0

Query: 120 RGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLN 179
           +G  +RGVR+RPWGK+AAEIRDP++ GAR+WLGTY TA++AALAYD+AA++MRG+KA LN
Sbjct: 95  KGRHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTYETAEEAALAYDKAAYRMRGSKALLN 154

Query: 180 FPHMIASTSNNDYPRRSCKRSSP 203
           FPH I   +  +  R + KR SP
Sbjct: 155 FPHRI-GLNEPEPVRLTAKRRSP 176

BLAST of Lsi05G022300 vs. ExPASy Swiss-Prot
Match: Q40476 (Ethylene-responsive transcription factor 1 OS=Nicotiana tabacum OX=4097 GN=ERF1 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.2e-22
Identity = 49/83 (59.04%), Postives = 64/83 (77.11%), Query Frame = 0

Query: 120 RGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLN 179
           RG  +RGVRRRPWGK+AAEIRDP++ GAR+WLGTY T ++AA+AYD+AA++MRG+KA LN
Sbjct: 101 RGRHYRGVRRRPWGKFAAEIRDPAKNGARVWLGTYETDEEAAIAYDKAAYRMRGSKAHLN 160

Query: 180 FPHMIASTSNNDYPRRSCKRSSP 203
           FPH I           + +R+SP
Sbjct: 161 FPHRIGLNEPEPVRVTAKRRASP 183

BLAST of Lsi05G022300 vs. ExPASy Swiss-Prot
Match: O04681 (Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicum OX=4081 GN=PTI5 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.2e-22
Identity = 50/68 (73.53%), Postives = 59/68 (86.76%), Query Frame = 0

Query: 121 GLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNF 180
           G ++RGVRRRPWGKYAAEIRD +R GAR+WLGT+ TA++AALAYDRAAF+MRGAKA LNF
Sbjct: 56  GKKYRGVRRRPWGKYAAEIRDSARHGARVWLGTFETAEEAALAYDRAAFRMRGAKALLNF 115

Query: 181 PHMIASTS 189
           P  I + S
Sbjct: 116 PSEIVNAS 123

BLAST of Lsi05G022300 vs. ExPASy Swiss-Prot
Match: Q9LW50 (Ethylene-responsive transcription factor 2 OS=Nicotiana sylvestris OX=4096 GN=ERF2 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.6e-22
Identity = 53/92 (57.61%), Postives = 69/92 (75.00%), Query Frame = 0

Query: 120 RGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLN 179
           +G  +RGVR+RPWGK+AAEIRDP++ GAR+WLGTY TA++AALAYD+AA++MRG+KA LN
Sbjct: 99  KGRHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTYETAEEAALAYDKAAYRMRGSKALLN 158

Query: 180 FPHMIASTSNNDYPRRSCKRSSPNCNSPRTPP 212
           FPH I   +  +  R + KR SP   S    P
Sbjct: 159 FPHRI-GLNEPEPVRLTVKRRSPEPASSSISP 189

BLAST of Lsi05G022300 vs. ExPASy TrEMBL
Match: A0A5D3DAY8 (Putative Ethylene-responsive element binding factor 13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00160 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 3.6e-43
Identity = 111/166 (66.87%), Postives = 129/166 (77.71%), Query Frame = 0

Query: 29  SNSNSSSLEG-SSDLFVLERIRRHLLEDELG----NNVDEEGRWEWGNNSTGGG-SKSND 88
           S+S+SSSLEG SSDLF+LE+IRRHLLED+LG    NN  E+G   +  + +G G   SN+
Sbjct: 4   SSSSSSSLEGSSSDLFLLEQIRRHLLEDDLGKGSTNNNVEDGIIRYDISCSGAGWEMSNN 63

Query: 89  NNNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDP 148
           NNNNMV     ++M       SSII   D+  ++ R RGLQFRGVRRRPWGKYAAEIRDP
Sbjct: 64  NNNNMV-----DQM-------SSII-IRDNPEEKSRPRGLQFRGVRRRPWGKYAAEIRDP 123

Query: 149 SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS 189
           SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPH+I+S +
Sbjct: 124 SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHIISSNN 156

BLAST of Lsi05G022300 vs. ExPASy TrEMBL
Match: A0A0A0L893 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G116730 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 1.1e-42
Identity = 107/165 (64.85%), Postives = 128/165 (77.58%), Query Frame = 0

Query: 30  NSNSSSLEG-SSDLFVLERIRRHLLEDELG-----NNVDEEGRWEWGNNSTGGGSKSNDN 89
           +S+SSSLEG SSDLF LE+IRRHLLED+LG     NNV E+G   +  + +G G + ++N
Sbjct: 3   SSSSSSLEGSSSDLFHLEQIRRHLLEDDLGKGSTHNNV-EDGIIRYDISRSGAGWEMSNN 62

Query: 90  NNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPS 149
           NNNMV     ++M       S II  + +++ Q R RGLQFRGVRRRPWGKYAAEIRDPS
Sbjct: 63  NNNMV-----DQM------SSIIIRDNPEEKSQPRPRGLQFRGVRRRPWGKYAAEIRDPS 122

Query: 150 RKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS 189
           +KGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPH+I+S +
Sbjct: 123 KKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHIISSNN 155

BLAST of Lsi05G022300 vs. ExPASy TrEMBL
Match: A0A6J1IH30 (ethylene-responsive transcription factor 13-like OS=Cucurbita maxima OX=3661 GN=LOC111472899 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.9e-36
Identity = 105/196 (53.57%), Postives = 120/196 (61.22%), Query Frame = 0

Query: 27  MWSNSNSSSLEGSSDLFVLERIRRHLLEDELGNNVDEEGRWEWGNNSTGGGSKSNDNNNN 86
           M SNSN S    SS++F+LE IRRHLLEDEL              +S GG  K      N
Sbjct: 1   MSSNSNCSQGSSSSEVFLLEWIRRHLLEDEL--------------DSVGGLGK------N 60

Query: 87  MVMVELGEEMRG--KSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSR 146
              ++ G+ +      GKGSSI             RGLQFRGVRRRPWGKYAAEIRDP+R
Sbjct: 61  TPSLKSGDGVTDIEAPGKGSSI-------------RGLQFRGVRRRPWGKYAAEIRDPNR 120

Query: 147 KGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS-----NNDYPRRSCKR 206
           KGARIWLGTY TA++AALAYDRAAFQMRGAKAKLNFPHMI S          YP+R+   
Sbjct: 121 KGARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRA--- 154

Query: 207 SSPNCNSPRTPPPLFN 216
                 SP +PPPLF+
Sbjct: 181 ------SPNSPPPLFS 154

BLAST of Lsi05G022300 vs. ExPASy TrEMBL
Match: A0A6J1GF81 (ethylene-responsive transcription factor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453384 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 3.7e-35
Identity = 104/194 (53.61%), Postives = 120/194 (61.86%), Query Frame = 0

Query: 27  MWSNSNSSSLEGSSDLFVLERIRRHLLEDELGNNVDEEGRWEWGNNSTGGGSKSNDNNNN 86
           M SNSN S    SS++F+LE IRRHLLEDEL    D  G         G  + S  +++ 
Sbjct: 1   MSSNSNCSQGSSSSEVFLLEWIRRHLLEDEL----DLVG-------GLGKNTPSLASSDG 60

Query: 87  MVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKG 146
           +  +E         GKGSSI             RGLQFRGVRRRPWGKYAAEIRDP+RKG
Sbjct: 61  VTDIE-------APGKGSSI-------------RGLQFRGVRRRPWGKYAAEIRDPNRKG 120

Query: 147 ARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS-----NNDYPRRSCKRSS 206
           ARIWLGTY TA++AALAYDRAAFQMRGAKAKLNFPHMI S          YP+R+     
Sbjct: 121 ARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRA----- 154

Query: 207 PNCNSPRTPPPLFN 216
               SP +PPPLF+
Sbjct: 181 ----SPDSPPPLFS 154

BLAST of Lsi05G022300 vs. ExPASy TrEMBL
Match: A0A5D2I7H1 (AP2/ERF domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_D12G117700v1 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 4.8e-27
Identity = 71/123 (57.72%), Postives = 85/123 (69.11%), Query Frame = 0

Query: 88  VMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGA 147
           V+V  GEE  G       I+    +    +R RG+ F+GVRRRPWGKYAAEIRDP R GA
Sbjct: 103 VVVSGGEEKEG-------IVNVERETNAPLRPRGMNFKGVRRRPWGKYAAEIRDPKRNGA 162

Query: 148 RIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSP 207
           RIWLGTY T +DAALAYDRAAF+MRGAKAKLNFPH+I S + +D  R   +R SP  ++ 
Sbjct: 163 RIWLGTYETPEDAALAYDRAAFEMRGAKAKLNFPHLIGS-NTSDPIRVGSRRRSPEPSAS 217

Query: 208 RTP 211
            TP
Sbjct: 223 TTP 217

BLAST of Lsi05G022300 vs. NCBI nr
Match: XP_004134277.2 (ethylene-responsive transcription factor 1 [Cucumis sativus])

HSP 1 Score: 194.1 bits (492), Expect = 1.2e-45
Identity = 119/191 (62.30%), Postives = 143/191 (74.87%), Query Frame = 0

Query: 9   DTECGSSKL-----RQKRIRSRKMWSNSNSSSLEG-SSDLFVLERIRRHLLEDELG---- 68
           +T+C S  L     + K+IRSRKM S S+SSSLEG SSDLF LE+IRRHLLED+LG    
Sbjct: 8   NTQCASGFLPITISKSKKIRSRKMLS-SSSSSLEGSSSDLFHLEQIRRHLLEDDLGKGST 67

Query: 69  -NNVDEEGRWEWGNNSTGGGSKSNDNNNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQI 128
            NNV E+G   +  + +G G + ++NNNNMV     ++M       S II  + +++ Q 
Sbjct: 68  HNNV-EDGIIRYDISRSGAGWEMSNNNNNMV-----DQM------SSIIIRDNPEEKSQP 127

Query: 129 RARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAK 188
           R RGLQFRGVRRRPWGKYAAEIRDPS+KGARIWLGTYHTAQDAALAYDRAAFQMRGAKAK
Sbjct: 128 RPRGLQFRGVRRRPWGKYAAEIRDPSKKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAK 185

BLAST of Lsi05G022300 vs. NCBI nr
Match: KAA0048804.1 (putative Ethylene-responsive element binding factor 13 [Cucumis melo var. makuwa] >TYK20756.1 putative Ethylene-responsive element binding factor 13 [Cucumis melo var. makuwa])

HSP 1 Score: 184.9 bits (468), Expect = 7.5e-43
Identity = 111/166 (66.87%), Postives = 129/166 (77.71%), Query Frame = 0

Query: 29  SNSNSSSLEG-SSDLFVLERIRRHLLEDELG----NNVDEEGRWEWGNNSTGGG-SKSND 88
           S+S+SSSLEG SSDLF+LE+IRRHLLED+LG    NN  E+G   +  + +G G   SN+
Sbjct: 4   SSSSSSSLEGSSSDLFLLEQIRRHLLEDDLGKGSTNNNVEDGIIRYDISCSGAGWEMSNN 63

Query: 89  NNNNMVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDP 148
           NNNNMV     ++M       SSII   D+  ++ R RGLQFRGVRRRPWGKYAAEIRDP
Sbjct: 64  NNNNMV-----DQM-------SSII-IRDNPEEKSRPRGLQFRGVRRRPWGKYAAEIRDP 123

Query: 149 SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS 189
           SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPH+I+S +
Sbjct: 124 SRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHIISSNN 156

BLAST of Lsi05G022300 vs. NCBI nr
Match: KAE8650208.1 (hypothetical protein Csa_010866 [Cucumis sativus])

HSP 1 Score: 166.0 bits (419), Expect = 3.6e-37
Identity = 98/160 (61.25%), Postives = 112/160 (70.00%), Query Frame = 0

Query: 30  NSNSSSLEG-SSDLFVLERIRRHLLEDELGNNVDEEGRWEWGNNSTGGGSKSNDNNNNMV 89
           +S+SSSLEG SSDLF LE+IRRHLLED+LG                    K + +NN   
Sbjct: 3   SSSSSSLEGSSSDLFHLEQIRRHLLEDDLG--------------------KGSTHNN--- 62

Query: 90  MVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGAR 149
            VE G            II  + +++ Q R RGLQFRGVRRRPWGKYAAEIRDPS+KGAR
Sbjct: 63  -VEDG------------IIRDNPEEKSQPRPRGLQFRGVRRRPWGKYAAEIRDPSKKGAR 122

Query: 150 IWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS 189
           IWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPH+I+S +
Sbjct: 123 IWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHIISSNN 126

BLAST of Lsi05G022300 vs. NCBI nr
Match: XP_022974259.1 (ethylene-responsive transcription factor 13-like [Cucurbita maxima])

HSP 1 Score: 162.5 bits (410), Expect = 4.0e-36
Identity = 105/196 (53.57%), Postives = 120/196 (61.22%), Query Frame = 0

Query: 27  MWSNSNSSSLEGSSDLFVLERIRRHLLEDELGNNVDEEGRWEWGNNSTGGGSKSNDNNNN 86
           M SNSN S    SS++F+LE IRRHLLEDEL              +S GG  K      N
Sbjct: 1   MSSNSNCSQGSSSSEVFLLEWIRRHLLEDEL--------------DSVGGLGK------N 60

Query: 87  MVMVELGEEMRG--KSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSR 146
              ++ G+ +      GKGSSI             RGLQFRGVRRRPWGKYAAEIRDP+R
Sbjct: 61  TPSLKSGDGVTDIEAPGKGSSI-------------RGLQFRGVRRRPWGKYAAEIRDPNR 120

Query: 147 KGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS-----NNDYPRRSCKR 206
           KGARIWLGTY TA++AALAYDRAAFQMRGAKAKLNFPHMI S          YP+R+   
Sbjct: 121 KGARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRA--- 154

Query: 207 SSPNCNSPRTPPPLFN 216
                 SP +PPPLF+
Sbjct: 181 ------SPNSPPPLFS 154

BLAST of Lsi05G022300 vs. NCBI nr
Match: XP_022950239.1 (ethylene-responsive transcription factor 1-like [Cucurbita moschata])

HSP 1 Score: 158.3 bits (399), Expect = 7.6e-35
Identity = 104/194 (53.61%), Postives = 120/194 (61.86%), Query Frame = 0

Query: 27  MWSNSNSSSLEGSSDLFVLERIRRHLLEDELGNNVDEEGRWEWGNNSTGGGSKSNDNNNN 86
           M SNSN S    SS++F+LE IRRHLLEDEL    D  G         G  + S  +++ 
Sbjct: 1   MSSNSNCSQGSSSSEVFLLEWIRRHLLEDEL----DLVG-------GLGKNTPSLASSDG 60

Query: 87  MVMVELGEEMRGKSGKGSSIIWSSDDQRDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKG 146
           +  +E         GKGSSI             RGLQFRGVRRRPWGKYAAEIRDP+RKG
Sbjct: 61  VTDIE-------APGKGSSI-------------RGLQFRGVRRRPWGKYAAEIRDPNRKG 120

Query: 147 ARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFPHMIASTS-----NNDYPRRSCKRSS 206
           ARIWLGTY TA++AALAYDRAAFQMRGAKAKLNFPHMI S          YP+R+     
Sbjct: 121 ARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRA----- 154

Query: 207 PNCNSPRTPPPLFN 216
               SP +PPPLF+
Sbjct: 181 ----SPDSPPPLFS 154

BLAST of Lsi05G022300 vs. TAIR 10
Match: AT2G44840.1 (ethylene-responsive element binding factor 13 )

HSP 1 Score: 119.8 bits (299), Expect = 2.8e-27
Identity = 55/83 (66.27%), Postives = 67/83 (80.72%), Query Frame = 0

Query: 118 RARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAK 177
           R +G+Q+RGVRRRPWGK+AAEIRDP + GAR+WLGTY T +DAA+AYDRAAFQ+RG+KAK
Sbjct: 86  RQKGMQYRGVRRRPWGKFAAEIRDPKKNGARVWLGTYETPEDAAVAYDRAAFQLRGSKAK 145

Query: 178 LNFPHMIASTSNNDYPRRSCKRS 201
           LNFPH+I S        R  +RS
Sbjct: 146 LNFPHLIGSCKYEPVRIRPRRRS 168

BLAST of Lsi05G022300 vs. TAIR 10
Match: AT4G17500.1 (ethylene responsive element binding factor 1 )

HSP 1 Score: 105.9 bits (263), Expect = 4.1e-23
Identity = 53/93 (56.99%), Postives = 71/93 (76.34%), Query Frame = 0

Query: 119 ARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKL 178
           A+G  +RGVR+RPWGK+AAEIRDP++ GAR+WLGT+ TA+DAALAYDRAAF+MRG++A L
Sbjct: 143 AKGKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDRAAFRMRGSRALL 202

Query: 179 NFPHMIASTSNNDYPRRSCKRSSPNCNSPRTPP 212
           NFP +  ++   D  R   KRSS + ++    P
Sbjct: 203 NFP-LRVNSGEPDPVRIKSKRSSFSSSNENGAP 234

BLAST of Lsi05G022300 vs. TAIR 10
Match: AT2G33710.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.7e-22
Identity = 57/117 (48.72%), Postives = 73/117 (62.39%), Query Frame = 0

Query: 103 GSSIIWSSDDQ------RDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHT 162
           G  I  S DDQ      +++  +R   +RGVR+RPWGK+AAEIRDP+ K AR+WLGT+ T
Sbjct: 43  GQPIPSSIDDQSSSLTLQEKSNSRQRNYRGVRQRPWGKWAAEIRDPN-KAARVWLGTFDT 102

Query: 163 AQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSPRTPPPL 214
           A++AALAYD+AAF+ RG KAKLNFP  I       YP  +          P  PPP+
Sbjct: 103 AEEAALAYDKAAFEFRGHKAKLNFPEHIRVNPTQLYPSPATSHDRIIVTPPSPPPPI 158

BLAST of Lsi05G022300 vs. TAIR 10
Match: AT2G33710.2 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.7e-22
Identity = 57/117 (48.72%), Postives = 73/117 (62.39%), Query Frame = 0

Query: 103 GSSIIWSSDDQ------RDQIRARGLQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHT 162
           G  I  S DDQ      +++  +R   +RGVR+RPWGK+AAEIRDP+ K AR+WLGT+ T
Sbjct: 43  GQPIPSSIDDQSSSLTLQEKSNSRQRNYRGVRQRPWGKWAAEIRDPN-KAARVWLGTFDT 102

Query: 163 AQDAALAYDRAAFQMRGAKAKLNFPHMIASTSNNDYPRRSCKRSSPNCNSPRTPPPL 214
           A++AALAYD+AAF+ RG KAKLNFP  I       YP  +          P  PPP+
Sbjct: 103 AEEAALAYDKAAFEFRGHKAKLNFPEHIRVNPTQLYPSPATSHDRIIVTPPSPPPPI 158

BLAST of Lsi05G022300 vs. TAIR 10
Match: AT3G23220.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 6.0e-22
Identity = 45/61 (73.77%), Postives = 55/61 (90.16%), Query Frame = 0

Query: 122 LQFRGVRRRPWGKYAAEIRDPSRKGARIWLGTYHTAQDAALAYDRAAFQMRGAKAKLNFP 181
           +++RGVR+RPWGKYAAEIRD +R GAR+WLGT++TA+DAA AYDRAAF MRG +A LNFP
Sbjct: 12  MKYRGVRKRPWGKYAAEIRDSARHGARVWLGTFNTAEDAARAYDRAAFGMRGQRAILNFP 71

Query: 182 H 183
           H
Sbjct: 72  H 72

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L9K13.9e-2666.27Ethylene-responsive transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=E... [more]
Q404796.9e-2361.45Ethylene-responsive transcription factor 2 OS=Nicotiana tabacum OX=4097 GN=ERF2 ... [more]
Q404761.2e-2259.04Ethylene-responsive transcription factor 1 OS=Nicotiana tabacum OX=4097 GN=ERF1 ... [more]
O046811.2e-2273.53Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicu... [more]
Q9LW502.6e-2257.61Ethylene-responsive transcription factor 2 OS=Nicotiana sylvestris OX=4096 GN=ER... [more]
Match NameE-valueIdentityDescription
A0A5D3DAY83.6e-4366.87Putative Ethylene-responsive element binding factor 13 OS=Cucumis melo var. maku... [more]
A0A0A0L8931.1e-4264.85AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G116730 PE=... [more]
A0A6J1IH301.9e-3653.57ethylene-responsive transcription factor 13-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1GF813.7e-3553.61ethylene-responsive transcription factor 1-like OS=Cucurbita moschata OX=3662 GN... [more]
A0A5D2I7H14.8e-2757.72AP2/ERF domain-containing protein OS=Gossypium tomentosum OX=34277 GN=ES332_D12G... [more]
Match NameE-valueIdentityDescription
XP_004134277.21.2e-4562.30ethylene-responsive transcription factor 1 [Cucumis sativus][more]
KAA0048804.17.5e-4366.87putative Ethylene-responsive element binding factor 13 [Cucumis melo var. makuwa... [more]
KAE8650208.13.6e-3761.25hypothetical protein Csa_010866 [Cucumis sativus][more]
XP_022974259.14.0e-3653.57ethylene-responsive transcription factor 13-like [Cucurbita maxima][more]
XP_022950239.17.6e-3553.61ethylene-responsive transcription factor 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G44840.12.8e-2766.27ethylene-responsive element binding factor 13 [more]
AT4G17500.14.1e-2356.99ethylene responsive element binding factor 1 [more]
AT2G33710.12.7e-2248.72Integrase-type DNA-binding superfamily protein [more]
AT2G33710.22.7e-2248.72Integrase-type DNA-binding superfamily protein [more]
AT3G23220.16.0e-2273.77Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 163..183
score: 56.97
coord: 124..135
score: 65.91
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 123..187
e-value: 1.0E-33
score: 128.0
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 124..173
e-value: 1.3E-13
score: 50.9
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 123..181
score: 23.182409
IPR001471AP2/ERF domainCDDcd00018AP2coord: 123..182
e-value: 3.43891E-27
score: 96.5718
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 122..183
e-value: 6.8E-28
score: 98.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..219
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..84
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availablePANTHERPTHR31190:SF128ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 1coord: 94..191
IPR044808Ethylene-responsive transcription factorPANTHERPTHR31190DNA-BINDING DOMAINcoord: 94..191
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 124..182

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G022300.1Lsi05G022300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009873 ethylene-activated signaling pathway
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity