Lsi05G021500 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G021500
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionserine/arginine-rich splicing factor RS31-like
Locationchr05: 28246946 .. 28249982 (-)
RNA-Seq ExpressionLsi05G021500
SyntenyLsi05G021500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGCCGATATTTGTTGGAAACTTTGGGTATGACACCCGGCAATCTGAGCTGGAGCGTTTATTCGCCAAATATGGGAGAGTTGAAAGGATCGACATGAAATCTGGTATGTTCCAATTTCTTTGTTCTAGTATGCAATGCCATTTTTAACGAACATGTTATAAAATTGGTCATAACTTGTGCTGATTATATTGTACGAAGGATCCCTTTTTGTGGTCAGCTTCGAGCAAATTGATTATGTTGAATATTGCTCTTAAAACCTTTTTTGGGTATTGCCAATGGTGTAATGAGTGGGTTGCAATGCTTGCTTAATTTATTTTTTGTTCAATGTGAAATTGTATCAAGATAATAGATTGTACCAAAAGGATCATCTTCATTTTGGTTAAGTTCATACGCATTGGACGGTGCATTTTGGATTATTGGTGTATGTTTTAATTTAAAATCTTTGATGTGCCCTAAGAATCATACTCCTCTTGTTGTGTGTCGAGTGTGGTAGGCTCACAAGTGTCTGTATGTATGCATTGATGGATGACACTTTGGTGTTGGTTTTACTTAGTATTGGCAAGTAGTATTCCTACTTTTAAGCTTCCTAGTTTGGAATTTTTTTTGTTACATGTGGACTTTTGTACTTTGATTTCATCATTCGAATCATCTTACCTTTTCCTTGTTAACTGACCTTGTTTACTCTGGGTATGAGTTTTTAATGATGTTTATTTTCTGGAAAATTTCCTTGGCCCTCACCCACCTTGCCTAACATCCTTGGTCCTGCAAAGTTATATTTCAAATAATGATTTTGGACATTTTTGGGTATAGGGGCTTACTGAATTATGGTATCTTAGTAAGAGGCTGCTCCACTGATACTAACATTAAGTGTTAAAGTAATATCACTTTTTTTTTTATCAATAATACTAAAATTGATGTTTTTTAAATAATAAAATCCAGTTCAGTGACATGGATGCATAGCTATTATGCTATGAATGACATCCAGTGGTCCATTTTTCTGATGAAAACTTAATGGCAGGCACAATTGCTATTTTTATGGGGAAAACCTCCATATAAGATGTTTCTTTTGACGCAGGAATTTCTTCTAAGGAAAATGAGCCAAGCTCCTATCTGGGCTCACCACCATGACACCATCTGTGCATAAAAATTAATTTGGAAGGAAACTTCCTGCTTGACTGCTTAGCTTCACCTACAGATAGCATCATCCTGATTTTTCTTCAATCAATCATCAATCATCATTCATCTACCTCATCACTTAAGTTGCAATACACCAGAATCGTCTTAATGGATTGATCTTTATCTCATGCCTCATCAATTAAGTTGCAATTCATCAGAATCGTGTTTATGGATTGATCTTTATCTCATGCCTTGCCTAGCTATCACCACTGATCATAGGACATCACTGAAATCTGATCATGCCTTCCATATGATAATAGTGGCTGAATGAAATCTGTCATGCCATGCCTTCTATTTCTGAACTCGTGCTGTGTGAAAGGCGAAGGATAGTTGAAATTTTGGATTAAAACCTTTAGTAGGCAACCATCTTAGTCCATCATGCAACGTCTCTACCGCTATATGTGCATCAGTCCTCCCGTATTTGCAGGTGTTGTATTTTTTATAGTTTATCCTAGGGAAAGAAATCACAAGGCTGAGTGACAATACCTGCTAAATGGATGATGCTTCAATAGCTTACCAGTTTTGTATATCTAATTGTTATTATTTGGCAGGTTTTGCCTTTGTTTACTTTGAAGATGAGAGGGATGCTGAAGATGCCATTCGGGGACTTGATAACTTGCCATTTGGTTATGATAGACGCAGATTGTCTGTGGAATGGGCTAGGGTAGACCTCTCTCACTACCTTCTTCTTTCCCTCCTTGTCCTTCCTTTTGGGTTTATGCATTTTAGAAGCTGGTTTATTTCATGTTCTGAAGATGGGCCATTTTATTCTAACTTATGGTTTGGATGGATAAGGGTGAACGAGGTCGTCATCGTGATGGGTCCAAGTCAATGGCAAATCAGAGGCCAACAAAAACCCTGTTTGTGATCAATTTTGACCCGATTCGGACAAGAGTTCGTGATATTGAAAGACACTTTGAACCTTATGGGAAGGTGCTCAATGTTCGCATTAGAAGGAACTTTGCATTTGTACAATTTGAGACGCAGGAGGATGCAACTAAAGCCCTTGAGTGTACTCACATGAGGTAAGCCGGGTTCTGATTCTCCTGCATGCTTTCTCTTTTCATTTGGTGTATTTATTTTCTTCTCTCTTATGGACGTGAAGTATTGTATTGTGCCTTGCAATTTTGCAGCAAAATATTAGACAGGGTTGTGTCAGTTGAATATGCTTTGAGGGATGACGGTGAGAGGGGTGATCCTTTTGATGAAAGCCCTCGAAGAGCAGGTTATGGGCGACCAGGGGATAGTCCCTACCGGAGGTCACCTAGTCCTGTGTTTCGCCGTCGGCCAAGTCCGGACTATGGGCGAGCTCGTAGCCCTGCTTATGATAGGTATAATGGGCCTTATGAACGACGTAGGAGTCCTGATTATGGTAGGAACCGAAGCCCTGAATATGGTGGTAGATTTCGCAGGTAATAATCTTTGTTCTTTGATAGTTGGCACAGTCTGCATTTAGCTCATAGTTCAAGCTAACCGCGGTTTTCCTCATGCCTTTGTTGACAGTCGTTCACCCATTCGGAGGTCAAGAACCTGAAGGAAAATCTGTTGATGAATTTTCAGTAGTTACATTCATAGGTTTAGGTCTGTGGAGTGTGTTAAGAGATAGTGACAAGTTCTTGTACAACCCTCGTATTTGACTATTTTAACTATTGTTGGTAGATTTGGGATTATGAAGAAAACACGGTTTTGTTTCAAGGCTATCTTGTCTTCACAATTGTTCACTTAGCAACGATCCAGCTTCCTTGCGCTTGAAATGTTCAAATGTTAATTCATGTTTAATTACACAACATTGGAACAGGTTGATCTTGGTTGATGTATCTGCATTCAATTCTTAAAAAAAAAAGTAATTTATTGTTTA

mRNA sequence

ATGAGGCCGATATTTGTTGGAAACTTTGGGTATGACACCCGGCAATCTGAGCTGGAGCGTTTATTCGCCAAATATGGGAGAGTTGAAAGGATCGACATGAAATCTGGTTTTGCCTTTGTTTACTTTGAAGATGAGAGGGATGCTGAAGATGCCATTCGGGGACTTGATAACTTGCCATTTGGTTATGATAGACGCAGATTGTCTGTGGAATGGGCTAGGGGTGAACGAGGTCGTCATCGTGATGGGTCCAAGTCAATGGCAAATCAGAGGCCAACAAAAACCCTGTTTGTGATCAATTTTGACCCGATTCGGACAAGAGTTCGTGATATTGAAAGACACTTTGAACCTTATGGGAAGGTGCTCAATGTTCGCATTAGAAGGAACTTTGCATTTGTACAATTTGAGACGCAGGAGGATGCAACTAAAGCCCTTGAGTGTACTCACATGAGCAAAATATTAGACAGGGTTGTGTCAGTTGAATATGCTTTGAGGGATGACGGTGAGAGGGGTGATCCTTTTGATGAAAGCCCTCGAAGAGCAGGTTATGGGCGACCAGGGGATAGTCCCTACCGGAGGTCACCTAGTCCTGTGTTTCGCCGTCGGCCAAGTCCGGACTATGGGCGAGCTCGTAGCCCTGCTTATGATAGGTATAATGGGCCTTATGAACGACGTAGGAGTCCTGATTATGGTAGGAACCGAAGCCCTGAATATGGTGTCGTTCACCCATTCGGAGGTCAAGAACCTGAAGGAAAATCTGTTGATGAATTTTCAGTAGTTACATTCATAGGTTTAGGTCTGTGGAGTGTGTTAAGAGATAGTGACAAGTTCTTGTACAACCCTCGTATTTGACTATTTTAACTATTGTTGGTAGATTTGGGATTATGAAGAAAACACGGTTTTGTTTCAAGGCTATCTTGTCTTCACAATTGTTCACTTAGCAACGATCCAGCTTCCTTGCGCTTGAAATGTTCAAATGTTAATTCATGTTTAATTACACAACATTGGAACAGGTTGATCTTGGTTGATGTATCTGCATTCAATTCTTAAAAAAAAAAGTAATTTATTGTTTA

Coding sequence (CDS)

ATGAGGCCGATATTTGTTGGAAACTTTGGGTATGACACCCGGCAATCTGAGCTGGAGCGTTTATTCGCCAAATATGGGAGAGTTGAAAGGATCGACATGAAATCTGGTTTTGCCTTTGTTTACTTTGAAGATGAGAGGGATGCTGAAGATGCCATTCGGGGACTTGATAACTTGCCATTTGGTTATGATAGACGCAGATTGTCTGTGGAATGGGCTAGGGGTGAACGAGGTCGTCATCGTGATGGGTCCAAGTCAATGGCAAATCAGAGGCCAACAAAAACCCTGTTTGTGATCAATTTTGACCCGATTCGGACAAGAGTTCGTGATATTGAAAGACACTTTGAACCTTATGGGAAGGTGCTCAATGTTCGCATTAGAAGGAACTTTGCATTTGTACAATTTGAGACGCAGGAGGATGCAACTAAAGCCCTTGAGTGTACTCACATGAGCAAAATATTAGACAGGGTTGTGTCAGTTGAATATGCTTTGAGGGATGACGGTGAGAGGGGTGATCCTTTTGATGAAAGCCCTCGAAGAGCAGGTTATGGGCGACCAGGGGATAGTCCCTACCGGAGGTCACCTAGTCCTGTGTTTCGCCGTCGGCCAAGTCCGGACTATGGGCGAGCTCGTAGCCCTGCTTATGATAGGTATAATGGGCCTTATGAACGACGTAGGAGTCCTGATTATGGTAGGAACCGAAGCCCTGAATATGGTGTCGTTCACCCATTCGGAGGTCAAGAACCTGAAGGAAAATCTGTTGATGAATTTTCAGTAGTTACATTCATAGGTTTAGGTCTGTGGAGTGTGTTAAGAGATAGTGACAAGTTCTTGTACAACCCTCGTATTTGA

Protein sequence

MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPFGYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKVLNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRAGYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYGVVHPFGGQEPEGKSVDEFSVVTFIGLGLWSVLRDSDKFLYNPRI
Homology
BLAST of Lsi05G021500 vs. ExPASy Swiss-Prot
Match: P92964 (Serine/arginine-rich splicing factor RS31 OS=Arabidopsis thaliana OX=3702 GN=RS31 PE=1 SV=2)

HSP 1 Score: 347.1 bits (889), Expect = 1.9e-94
Identity = 182/244 (74.59%), Postives = 201/244 (82.38%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRP+FVGNF Y+TRQS+LERLF KYGRV+R+DMKSG+AFVYFEDERDAEDAIR LDN PF
Sbjct: 1   MRPVFVGNFEYETRQSDLERLFDKYGRVDRVDMKSGYAFVYFEDERDAEDAIRKLDNFPF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GY++RRLSVEWA+GERGR R  +K+ +N +PTKTLFVINFDPIRT+  DIE+HFEPYGKV
Sbjct: 61  GYEKRRLSVEWAKGERGRPRGDAKAPSNLKPTKTLFVINFDPIRTKEHDIEKHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGD-PFDESPRR 180
            NVRIRRNF+FVQFETQEDATKALE T  SKILDRVVSVEYAL+DD ER D     SPRR
Sbjct: 121 TNVRIRRNFSFVQFETQEDATKALEATQRSKILDRVVSVEYALKDDDERDDRNGGRSPRR 180

Query: 181 A---GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGP--YERRRSPDYGRNRS 239
           +    Y R     Y R PSP   RRPSPDYGRARSP YDRY GP  YERRRSPDYGR RS
Sbjct: 181 SLSPVYRRRPSPDYGRRPSPGQGRRPSPDYGRARSPEYDRYKGPAAYERRRSPDYGR-RS 240

BLAST of Lsi05G021500 vs. ExPASy Swiss-Prot
Match: Q9ZPX8 (Serine/arginine-rich splicing factor RS31A OS=Arabidopsis thaliana OX=3702 GN=RS31A PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 5.8e-91
Identity = 176/243 (72.43%), Postives = 200/243 (82.30%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MR ++VGNF YDTR S+LERLF+K+GRV+R+DMKSG+AFVYFEDERDAEDAIR  DN  F
Sbjct: 1   MRHVYVGNFDYDTRHSDLERLFSKFGRVKRVDMKSGYAFVYFEDERDAEDAIRRTDNTTF 60

Query: 61  GYDRRRLSVEWAR---GERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPY 120
           GY RR+LSVEWA+   GERG+ RDG K+++NQRPTKTLFVINFDPIRTR RD+ERHFEPY
Sbjct: 61  GYGRRKLSVEWAKDFQGERGKPRDG-KAVSNQRPTKTLFVINFDPIRTRERDMERHFEPY 120

Query: 121 GKVLNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESP 180
           GKVLNVR+RRNFAFVQF TQEDATKAL+ TH SK+LD+VVSVEYALR+ GER D +  S 
Sbjct: 121 GKVLNVRMRRNFAFVQFATQEDATKALDSTHNSKLLDKVVSVEYALREAGEREDRYAGSR 180

Query: 181 RRAGYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNG--PYERRRSPDYGRNRSP 239
           R            RRSPSPV+RRRPSPDY R RSP YDRY G  PYERR+SPDYGR RS 
Sbjct: 181 R------------RRSPSPVYRRRPSPDYTRRRSPEYDRYKGPAPYERRKSPDYGR-RSS 229

BLAST of Lsi05G021500 vs. ExPASy Swiss-Prot
Match: P92965 (Serine/arginine-rich splicing factor RS40 OS=Arabidopsis thaliana OX=3702 GN=RS40 PE=1 SV=2)

HSP 1 Score: 267.3 bits (682), Expect = 1.9e-70
Identity = 144/241 (59.75%), Postives = 174/241 (72.20%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           M+P+F GNF YD R+ +LERLF KYG+VER+DMK+GFAFVY EDERDAEDAIR LD   F
Sbjct: 1   MKPVFCGNFEYDAREGDLERLFRKYGKVERVDMKAGFAFVYMEDERDAEDAIRALDRFEF 60

Query: 61  GYDRRRLSVEWARGERG---RHRDGS-KSMANQRPTKTLFVINFDPIRTRVRDIERHFEP 120
           G   RRL VEW + ERG   R   GS +S ++ RP+KTLFVINFD   TR RD+E+HFEP
Sbjct: 61  GRKGRRLRVEWTKSERGGDKRSGGGSRRSSSSMRPSKTLFVINFDADNTRTRDLEKHFEP 120

Query: 121 YGKVLNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDES 180
           YGK++NVRIRRNFAF+Q+E QEDAT+AL+ ++ SK++D+V+SVEYA++DD  RG+    S
Sbjct: 121 YGKIVNVRIRRNFAFIQYEAQEDATRALDASNNSKLMDKVISVEYAVKDDDARGN--GHS 180

Query: 181 PRRAGYGRPGDSPYRRSPSPVFRRRPSPDYGRARSP--AYDRYNGPYERRRSPDYGRNRS 236
           P R     P     RRSPSP  R R SPDYGR  SP  AY +       R SPDYGR RS
Sbjct: 181 PERRRDRSP--ERRRRSPSPYKRERGSPDYGRGASPVAAYRK------ERTSPDYGRRRS 231

BLAST of Lsi05G021500 vs. ExPASy Swiss-Prot
Match: P92966 (Serine/arginine-rich splicing factor RS41 OS=Arabidopsis thaliana OX=3702 GN=RS41 PE=1 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 2.5e-70
Identity = 139/236 (58.90%), Postives = 169/236 (71.61%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           M+P+F GNF YD R+S+LERLF KYG+VER+DMK+GFAFVY EDERDAEDAIR LD   +
Sbjct: 1   MKPVFCGNFEYDARESDLERLFRKYGKVERVDMKAGFAFVYMEDERDAEDAIRALDRFEY 60

Query: 61  GYDRRRLSVEWARGER---GRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPY 120
           G   RRL VEW + +R   GR     +S +  RP+KTLFVINFD   TR RD+ERHFEPY
Sbjct: 61  GRTGRRLRVEWTKNDRGGAGRSGGSRRSSSGLRPSKTLFVINFDAQNTRTRDLERHFEPY 120

Query: 121 GKVLNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESP 180
           GK++NVRIRRNFAF+Q+E QEDAT+AL+ T+ SK++D+V+SVEYA++DD  RG+ +    
Sbjct: 121 GKIVNVRIRRNFAFIQYEAQEDATRALDATNSSKLMDKVISVEYAVKDDDSRGNGYSPER 180

Query: 181 RRAGYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNR 234
           RR    R  D   RRSPSP  R R SPDYGR  SP   +       R SPDYGR R
Sbjct: 181 RR---DRSPDR-RRRSPSPYRRERGSPDYGRGASPVAHK-----RERTSPDYGRGR 227

BLAST of Lsi05G021500 vs. ExPASy Swiss-Prot
Match: Q9NEW6 (Probable splicing factor, arginine/serine-rich 3 OS=Caenorhabditis elegans OX=6239 GN=rsp-3 PE=1 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 2.8e-16
Identity = 81/258 (31.40%), Postives = 111/258 (43.02%), Query Frame = 0

Query: 4   IFVGNFGYDTRQSELERLFAKYGRVERIDMKSG----FAFVYFEDERDAEDAIRGLDNLP 63
           ++VGN   D R+ E+E +F KYGR++ +D+KSG    FAFV FED RDAEDA+R  D   
Sbjct: 11  VYVGNLPGDVREKEVEDIFHKYGRIKYVDIKSGRGPAFAFVEFEDHRDAEDAVRARDG-- 70

Query: 64  FGYDRRRLSVEWARG------------ERGRH--------RDGSKSMANQRPTKTLFVIN 123
           + +D RR+ VE+ RG            + G H        R G +    QR T    ++ 
Sbjct: 71  YEFDGRRIRVEFTRGVGPRGPGGRPLQDGGDHRGGDFRGGRGGGRGGGPQRRTGYRVIVE 130

Query: 124 FDPIRTRVRDIERHFEPYGKVLNVRIRRN-FAFVQFETQEDATKALECTHMSKILDRVVS 183
             P     +D++ H    G V    + R+    V+F   ED   A+     +K       
Sbjct: 131 GLPPTGSWQDLKDHMRDAGDVCYADVARDGTGVVEFTRYEDVKYAVRKLDDTKFRSHEGE 190

Query: 184 VEY-ALRDDGERGDPFDESPRRAGYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRY 236
             Y  +R+D   G          G    G     RS SP   RR SP Y   RS +  R 
Sbjct: 191 TAYIRVREDNSSG----------GGSGGGGRDRSRSRSPRAERRASPKYSPRRSRSRSR- 250

BLAST of Lsi05G021500 vs. ExPASy TrEMBL
Match: A0A5D3DBI0 (Serine/arginine-rich splicing factor RS31 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00820 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 7.1e-132
Identity = 236/239 (98.74%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLF+KYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF
Sbjct: 1   MRPIFVGNFGYDTRQSELERLFSKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA
Sbjct: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180

Query: 181 -GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            GYGRPGDSPYRRSPSPVFRRRPSPDYGRA SPAYDRYNGPYERRRSPDYGRNRSPEYG
Sbjct: 181 GGYGRPGDSPYRRSPSPVFRRRPSPDYGRAHSPAYDRYNGPYERRRSPDYGRNRSPEYG 239

BLAST of Lsi05G021500 vs. ExPASy TrEMBL
Match: A0A0A0L8H4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119350 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 7.1e-132
Identity = 236/239 (98.74%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLF+KYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF
Sbjct: 1   MRPIFVGNFGYDTRQSELERLFSKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA
Sbjct: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180

Query: 181 -GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            GYGRPGDSPYRRSPSPVFRRRPSPDYGRA SPAYDRYNGPYERRRSPDYGRNRSPEYG
Sbjct: 181 GGYGRPGDSPYRRSPSPVFRRRPSPDYGRAHSPAYDRYNGPYERRRSPDYGRNRSPEYG 239

BLAST of Lsi05G021500 vs. ExPASy TrEMBL
Match: A0A1S3AUZ7 (serine/arginine-rich splicing factor RS31-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483164 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 7.1e-132
Identity = 236/239 (98.74%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLF+KYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF
Sbjct: 1   MRPIFVGNFGYDTRQSELERLFSKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA
Sbjct: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180

Query: 181 -GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            GYGRPGDSPYRRSPSPVFRRRPSPDYGRA SPAYDRYNGPYERRRSPDYGRNRSPEYG
Sbjct: 181 GGYGRPGDSPYRRSPSPVFRRRPSPDYGRAHSPAYDRYNGPYERRRSPDYGRNRSPEYG 239

BLAST of Lsi05G021500 vs. ExPASy TrEMBL
Match: A0A6J1D279 (serine/arginine-rich splicing factor RS31-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016334 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 5.1e-130
Identity = 233/239 (97.49%), Postives = 235/239 (98.33%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDN+ F
Sbjct: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNMAF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGRHRDGSKS+ANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 61  GYDRRRLSVEWARGERGRHRDGSKSIANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA
Sbjct: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180

Query: 181 GYGRPGD-SPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            YGRPGD SPYRRSPSP FRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG
Sbjct: 181 AYGRPGDSSPYRRSPSPAFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239

BLAST of Lsi05G021500 vs. ExPASy TrEMBL
Match: A0A6J1ECU9 (serine/arginine-rich splicing factor RS31-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432011 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.4e-126
Identity = 224/238 (94.12%), Postives = 232/238 (97.48%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFE+ERDAED+IR LDN+PF
Sbjct: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEEERDAEDSIRSLDNMPF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGR RDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 61  GYDRRRLSVEWARGERGRSRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQE+ATKALECTHMSKILDRVVSVEYALRDDGERGD +D+SPRRA
Sbjct: 121 LNVRIRRNFAFVQFETQEEATKALECTHMSKILDRVVSVEYALRDDGERGDHYDDSPRRA 180

Query: 181 GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            YGR GDSPYRRSPSPV+ RRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSP+YG
Sbjct: 181 AYGRLGDSPYRRSPSPVYHRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPDYG 238

BLAST of Lsi05G021500 vs. NCBI nr
Match: XP_038893478.1 (serine/arginine-rich splicing factor RS31 isoform X1 [Benincasa hispida])

HSP 1 Score: 487.6 bits (1254), Expect = 7.0e-134
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF
Sbjct: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA
Sbjct: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180

Query: 181 GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
           GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG
Sbjct: 181 GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 238

BLAST of Lsi05G021500 vs. NCBI nr
Match: KAE8650222.1 (hypothetical protein Csa_011015 [Cucumis sativus])

HSP 1 Score: 479.9 bits (1234), Expect = 1.5e-131
Identity = 236/239 (98.74%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLF+KYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF
Sbjct: 681 MRPIFVGNFGYDTRQSELERLFSKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 740

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 741 GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 800

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA
Sbjct: 801 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 860

Query: 181 -GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            GYGRPGDSPYRRSPSPVFRRRPSPDYGRA SPAYDRYNGPYERRRSPDYGRNRSPEYG
Sbjct: 861 GGYGRPGDSPYRRSPSPVFRRRPSPDYGRAHSPAYDRYNGPYERRRSPDYGRNRSPEYG 919

BLAST of Lsi05G021500 vs. NCBI nr
Match: XP_004133800.1 (serine/arginine-rich splicing factor RS31 isoform X1 [Cucumis sativus] >XP_008437857.1 PREDICTED: serine/arginine-rich splicing factor RS31-like isoform X1 [Cucumis melo] >KAA0048868.1 serine/arginine-rich splicing factor RS31 isoform X2 [Cucumis melo var. makuwa] >TYK20820.1 serine/arginine-rich splicing factor RS31 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 479.9 bits (1234), Expect = 1.5e-131
Identity = 236/239 (98.74%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLF+KYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF
Sbjct: 1   MRPIFVGNFGYDTRQSELERLFSKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA
Sbjct: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180

Query: 181 -GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            GYGRPGDSPYRRSPSPVFRRRPSPDYGRA SPAYDRYNGPYERRRSPDYGRNRSPEYG
Sbjct: 181 GGYGRPGDSPYRRSPSPVFRRRPSPDYGRAHSPAYDRYNGPYERRRSPDYGRNRSPEYG 239

BLAST of Lsi05G021500 vs. NCBI nr
Match: XP_022147391.1 (serine/arginine-rich splicing factor RS31-like isoform X1 [Momordica charantia])

HSP 1 Score: 473.8 bits (1218), Expect = 1.0e-129
Identity = 233/239 (97.49%), Postives = 235/239 (98.33%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDN+ F
Sbjct: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNMAF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGRHRDGSKS+ANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 61  GYDRRRLSVEWARGERGRHRDGSKSIANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA
Sbjct: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180

Query: 181 GYGRPGD-SPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            YGRPGD SPYRRSPSP FRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG
Sbjct: 181 AYGRPGDSSPYRRSPSPAFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239

BLAST of Lsi05G021500 vs. NCBI nr
Match: KAG7019084.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 460.7 bits (1184), Expect = 9.2e-126
Identity = 224/238 (94.12%), Postives = 232/238 (97.48%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFE+ERDAED+IR LDN+PF
Sbjct: 564 MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEEERDAEDSIRSLDNMPF 623

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GYDRRRLSVEWARGERGR RDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV
Sbjct: 624 GYDRRRLSVEWARGERGRSRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 683

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESPRRA 180
           LNVRIRRNFAFVQFETQE+ATKALECTHMSKILDRVVSVEYALRDDGERGD +D+SPRRA
Sbjct: 684 LNVRIRRNFAFVQFETQEEATKALECTHMSKILDRVVSVEYALRDDGERGDHYDDSPRRA 743

Query: 181 GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPEYG 239
            YGR GDSPYRRSPSPV+ RRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSP+YG
Sbjct: 744 AYGRLGDSPYRRSPSPVYHRRPSPDYGRARSPAYDRYNGPYERRRSPDYGRNRSPDYG 801

BLAST of Lsi05G021500 vs. TAIR 10
Match: AT3G61860.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.4e-95
Identity = 182/244 (74.59%), Postives = 201/244 (82.38%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MRP+FVGNF Y+TRQS+LERLF KYGRV+R+DMKSG+AFVYFEDERDAEDAIR LDN PF
Sbjct: 1   MRPVFVGNFEYETRQSDLERLFDKYGRVDRVDMKSGYAFVYFEDERDAEDAIRKLDNFPF 60

Query: 61  GYDRRRLSVEWARGERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPYGKV 120
           GY++RRLSVEWA+GERGR R  +K+ +N +PTKTLFVINFDPIRT+  DIE+HFEPYGKV
Sbjct: 61  GYEKRRLSVEWAKGERGRPRGDAKAPSNLKPTKTLFVINFDPIRTKEHDIEKHFEPYGKV 120

Query: 121 LNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGD-PFDESPRR 180
            NVRIRRNF+FVQFETQEDATKALE T  SKILDRVVSVEYAL+DD ER D     SPRR
Sbjct: 121 TNVRIRRNFSFVQFETQEDATKALEATQRSKILDRVVSVEYALKDDDERDDRNGGRSPRR 180

Query: 181 A---GYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNGP--YERRRSPDYGRNRS 239
           +    Y R     Y R PSP   RRPSPDYGRARSP YDRY GP  YERRRSPDYGR RS
Sbjct: 181 SLSPVYRRRPSPDYGRRPSPGQGRRPSPDYGRARSPEYDRYKGPAAYERRRSPDYGR-RS 240

BLAST of Lsi05G021500 vs. TAIR 10
Match: AT2G46610.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 335.5 bits (859), Expect = 4.1e-92
Identity = 176/243 (72.43%), Postives = 200/243 (82.30%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           MR ++VGNF YDTR S+LERLF+K+GRV+R+DMKSG+AFVYFEDERDAEDAIR  DN  F
Sbjct: 1   MRHVYVGNFDYDTRHSDLERLFSKFGRVKRVDMKSGYAFVYFEDERDAEDAIRRTDNTTF 60

Query: 61  GYDRRRLSVEWAR---GERGRHRDGSKSMANQRPTKTLFVINFDPIRTRVRDIERHFEPY 120
           GY RR+LSVEWA+   GERG+ RDG K+++NQRPTKTLFVINFDPIRTR RD+ERHFEPY
Sbjct: 61  GYGRRKLSVEWAKDFQGERGKPRDG-KAVSNQRPTKTLFVINFDPIRTRERDMERHFEPY 120

Query: 121 GKVLNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDESP 180
           GKVLNVR+RRNFAFVQF TQEDATKAL+ TH SK+LD+VVSVEYALR+ GER D +  S 
Sbjct: 121 GKVLNVRMRRNFAFVQFATQEDATKALDSTHNSKLLDKVVSVEYALREAGEREDRYAGSR 180

Query: 181 RRAGYGRPGDSPYRRSPSPVFRRRPSPDYGRARSPAYDRYNG--PYERRRSPDYGRNRSP 239
           R            RRSPSPV+RRRPSPDY R RSP YDRY G  PYERR+SPDYGR RS 
Sbjct: 181 R------------RRSPSPVYRRRPSPDYTRRRSPEYDRYKGPAPYERRKSPDYGR-RSS 229

BLAST of Lsi05G021500 vs. TAIR 10
Match: AT2G46610.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 281.2 bits (718), Expect = 9.3e-76
Identity = 151/213 (70.89%), Postives = 171/213 (80.28%), Query Frame = 0

Query: 31  IDMKSGFAFVYFEDERDAEDAIRGLDNLPFGYDRRRLSVEWAR---GERGRHRDGSKSMA 90
           + + +G+AFVYFEDERDAEDAIR  DN  FGY RR+LSVEWA+   GERG+ RDG K+++
Sbjct: 5   LHIDAGYAFVYFEDERDAEDAIRRTDNTTFGYGRRKLSVEWAKDFQGERGKPRDG-KAVS 64

Query: 91  NQRPTKTLFVINFDPIRTRVRDIERHFEPYGKVLNVRIRRNFAFVQFETQEDATKALECT 150
           NQRPTKTLFVINFDPIRTR RD+ERHFEPYGKVLNVR+RRNFAFVQF TQEDATKAL+ T
Sbjct: 65  NQRPTKTLFVINFDPIRTRERDMERHFEPYGKVLNVRMRRNFAFVQFATQEDATKALDST 124

Query: 151 HMSKILDRVVSVEYALRDDGERGDPFDESPRRAGYGRPGDSPYRRSPSPVFRRRPSPDYG 210
           H SK+LD+VVSVEYALR+ GER D +  S R            RRSPSPV+RRRPSPDY 
Sbjct: 125 HNSKLLDKVVSVEYALREAGEREDRYAGSRR------------RRSPSPVYRRRPSPDYT 184

Query: 211 RARSPAYDRYNG--PYERRRSPDYGRNRSPEYG 239
           R RSP YDRY G  PYERR+SPDYGR RS +YG
Sbjct: 185 RRRSPEYDRYKGPAPYERRKSPDYGR-RSSDYG 203

BLAST of Lsi05G021500 vs. TAIR 10
Match: AT4G25500.1 (arginine/serine-rich splicing factor 35 )

HSP 1 Score: 267.3 bits (682), Expect = 1.4e-71
Identity = 144/241 (59.75%), Postives = 174/241 (72.20%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           M+P+F GNF YD R+ +LERLF KYG+VER+DMK+GFAFVY EDERDAEDAIR LD   F
Sbjct: 1   MKPVFCGNFEYDAREGDLERLFRKYGKVERVDMKAGFAFVYMEDERDAEDAIRALDRFEF 60

Query: 61  GYDRRRLSVEWARGERG---RHRDGS-KSMANQRPTKTLFVINFDPIRTRVRDIERHFEP 120
           G   RRL VEW + ERG   R   GS +S ++ RP+KTLFVINFD   TR RD+E+HFEP
Sbjct: 61  GRKGRRLRVEWTKSERGGDKRSGGGSRRSSSSMRPSKTLFVINFDADNTRTRDLEKHFEP 120

Query: 121 YGKVLNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDES 180
           YGK++NVRIRRNFAF+Q+E QEDAT+AL+ ++ SK++D+V+SVEYA++DD  RG+    S
Sbjct: 121 YGKIVNVRIRRNFAFIQYEAQEDATRALDASNNSKLMDKVISVEYAVKDDDARGN--GHS 180

Query: 181 PRRAGYGRPGDSPYRRSPSPVFRRRPSPDYGRARSP--AYDRYNGPYERRRSPDYGRNRS 236
           P R     P     RRSPSP  R R SPDYGR  SP  AY +       R SPDYGR RS
Sbjct: 181 PERRRDRSP--ERRRRSPSPYKRERGSPDYGRGASPVAAYRK------ERTSPDYGRRRS 231

BLAST of Lsi05G021500 vs. TAIR 10
Match: AT4G25500.4 (arginine/serine-rich splicing factor 35 )

HSP 1 Score: 267.3 bits (682), Expect = 1.4e-71
Identity = 144/241 (59.75%), Postives = 174/241 (72.20%), Query Frame = 0

Query: 1   MRPIFVGNFGYDTRQSELERLFAKYGRVERIDMKSGFAFVYFEDERDAEDAIRGLDNLPF 60
           M+P+F GNF YD R+ +LERLF KYG+VER+DMK+GFAFVY EDERDAEDAIR LD   F
Sbjct: 1   MKPVFCGNFEYDAREGDLERLFRKYGKVERVDMKAGFAFVYMEDERDAEDAIRALDRFEF 60

Query: 61  GYDRRRLSVEWARGERG---RHRDGS-KSMANQRPTKTLFVINFDPIRTRVRDIERHFEP 120
           G   RRL VEW + ERG   R   GS +S ++ RP+KTLFVINFD   TR RD+E+HFEP
Sbjct: 61  GRKGRRLRVEWTKSERGGDKRSGGGSRRSSSSMRPSKTLFVINFDADNTRTRDLEKHFEP 120

Query: 121 YGKVLNVRIRRNFAFVQFETQEDATKALECTHMSKILDRVVSVEYALRDDGERGDPFDES 180
           YGK++NVRIRRNFAF+Q+E QEDAT+AL+ ++ SK++D+V+SVEYA++DD  RG+    S
Sbjct: 121 YGKIVNVRIRRNFAFIQYEAQEDATRALDASNNSKLMDKVISVEYAVKDDDARGN--GHS 180

Query: 181 PRRAGYGRPGDSPYRRSPSPVFRRRPSPDYGRARSP--AYDRYNGPYERRRSPDYGRNRS 236
           P R     P     RRSPSP  R R SPDYGR  SP  AY +       R SPDYGR RS
Sbjct: 181 PERRRDRSP--ERRRRSPSPYKRERGSPDYGRGASPVAAYRK------ERTSPDYGRRRS 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P929641.9e-9474.59Serine/arginine-rich splicing factor RS31 OS=Arabidopsis thaliana OX=3702 GN=RS3... [more]
Q9ZPX85.8e-9172.43Serine/arginine-rich splicing factor RS31A OS=Arabidopsis thaliana OX=3702 GN=RS... [more]
P929651.9e-7059.75Serine/arginine-rich splicing factor RS40 OS=Arabidopsis thaliana OX=3702 GN=RS4... [more]
P929662.5e-7058.90Serine/arginine-rich splicing factor RS41 OS=Arabidopsis thaliana OX=3702 GN=RS4... [more]
Q9NEW62.8e-1631.40Probable splicing factor, arginine/serine-rich 3 OS=Caenorhabditis elegans OX=62... [more]
Match NameE-valueIdentityDescription
A0A5D3DBI07.1e-13298.74Serine/arginine-rich splicing factor RS31 isoform X2 OS=Cucumis melo var. makuwa... [more]
A0A0A0L8H47.1e-13298.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119350 PE=4 SV=1[more]
A0A1S3AUZ77.1e-13298.74serine/arginine-rich splicing factor RS31-like isoform X1 OS=Cucumis melo OX=365... [more]
A0A6J1D2795.1e-13097.49serine/arginine-rich splicing factor RS31-like isoform X1 OS=Momordica charantia... [more]
A0A6J1ECU94.4e-12694.12serine/arginine-rich splicing factor RS31-like isoform X1 OS=Cucurbita moschata ... [more]
Match NameE-valueIdentityDescription
XP_038893478.17.0e-134100.00serine/arginine-rich splicing factor RS31 isoform X1 [Benincasa hispida][more]
KAE8650222.11.5e-13198.74hypothetical protein Csa_011015 [Cucumis sativus][more]
XP_004133800.11.5e-13198.74serine/arginine-rich splicing factor RS31 isoform X1 [Cucumis sativus] >XP_00843... [more]
XP_022147391.11.0e-12997.49serine/arginine-rich splicing factor RS31-like isoform X1 [Momordica charantia][more]
KAG7019084.19.2e-12694.12Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
AT3G61860.11.4e-9574.59RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT2G46610.14.1e-9272.43RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT2G46610.29.3e-7670.89RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G25500.11.4e-7159.75arginine/serine-rich splicing factor 35 [more]
AT4G25500.41.4e-7159.75arginine/serine-rich splicing factor 35 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 94..160
e-value: 3.3E-17
score: 73.2
coord: 3..70
e-value: 7.5E-16
score: 68.7
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 4..59
e-value: 2.2E-16
score: 59.5
coord: 95..157
e-value: 3.9E-12
score: 45.8
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 93..164
score: 15.220978
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 2..74
score: 17.438179
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 85..172
e-value: 4.1E-19
score: 70.6
coord: 1..84
e-value: 1.3E-21
score: 78.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 166..249
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 166..181
NoneNo IPR availablePANTHERPTHR23147SERINE/ARGININE RICH SPLICING FACTORcoord: 4..213
NoneNo IPR availableCDDcd12466RRM2_AtRSp31_likecoord: 94..163
e-value: 8.87973E-42
score: 136.524
IPR029537Arginine/serine-rich-splicing factor RSP31/31APANTHERPTHR23147:SF146SERINE/ARGININE-RICH SPLICING FACTOR RS31-RELATEDcoord: 4..213
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 2..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G021500.1Lsi05G021500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0016607 nuclear speck
cellular_component GO:0005681 spliceosomal complex
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding