Lsi05G016260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G016260
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein kinase domain-containing protein
Locationchr05: 23901512 .. 23905951 (-)
RNA-Seq ExpressionLsi05G016260
SyntenyLsi05G016260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTTGTTAAAACTCTATTTAGAATATTTGTGACAAGTCTCGTACTGGCATACAATTTTCTCTTACATTTTTTTAGTCATATTTACTTTAAATTACAAATTTATTATACATTCAATCAGGTAATTTATCTTCCTCGATAATATATACACACACATTTTTATAGTATTGCCATGAAATTAAATTGAATTACATTATTGGAATTAGGTTAAAAAGAAGGAGGAATCTTGGATCATCCATCCGTTGGATACATACATACAGGTAATTCAGTTGAAAAAGAATTTCATCTCCGGGCTCTGTGTTTATCTTTCGCTAATTTTAGTATAAGCACTTTCATTGAATTTGAAAGAGTCAGTATCGATTCCGAACCCTATGATATAGTGAAATCCCCGCGCTTTTCTCTTCGTCGTTCCCAAAATCCTGTGGTGTTACGGTTCCCATTGCTGGTTTCTTCTTTTTTAGAAAATTTGGTTTCGATTCTTCGTTGCCTTGCGATTCTCTGTTCTTCCATAACCCCTTTCGTGTCCTGTGGGTTTGCTGCAGCGAACCCATTGGACTAAAACAACTAGAATCGAAGTTTTTTTTCAATTCTCTGCTTTTCCATGATAGCGCCAATGGCGCTTCAGTGATTTTTTGTTGGCTTTGTATCTCTTTCTTCTACCTTTGAATCTTAAAAGCATAATACCCTACTTACCGCCTCTTTCATTATCATTCGCTGTCTTAGTCAGCATTTACGGTCTTCTTCTTTCTGGCGAGGGCTCCTTTAGGGTCTGCGGGTCCGATTTTGATGAGAGGTAATTACTGTTTTTGAATTGTCTTCTTCCTTTTTTTTTTGCCCTTTTCGTGTTTTACCTTGATTTTTAGGGTTATGGGTGCCTGGTATTGGCCTTTGGGGGTAGTCATAATTTGGGTCGTTTTCTGGAGGTTTACTTTAATATTTTATTCCAAATTGAATTTTTAACTCCATCGTCAACTGTGGTTTTCCTGATAGAGAAGAATAATCTTTTTTATTAGCCTTTTGTATTCTCTGACTATTGTTATTTCTGATATAGCTGCCTTTTCTGTTCCAGAAGGTTAATTATTGTTATTATTTATTTATTTTTATTGGGGCATTGGTCAACATTGGAGGTAGTTCCTGTTTTTATTGGGTGGTTGTGTTGGAAATTTGGGTACTCTCTCTCTTTTTGAAAGATTTAGCTGCCTATTGTTTCGAGTTGAACTTTGTTGGTGAAGTAGATTCAAATTTGGGAGAAGTCTGAATTTGTTCCTTTATTGGTTTATTTTTACTTTTTAACAGGTTGAAGGATTGTCTATGATAATGGGAATTATGACAGACAACAACATTCTGTTCAGTTATCACTTTACCGGGCTGCAATCTTCACCCTGTTTGATCTCTCAGGTCTAAAATTCCTTGTATTCAAGGTGTACTATTTGAGTTATCTGTGTTGATCCTAGCAATGGATTCGAAAGGAAATGATATGGGGGGTACTGCGATCCCAAAGGAGACACAGGGCCAGGATAGAACTCTGAACTCAAAGGTGGCTGGTATGGGAAGCATTAGCAGCAAAGATATGATATTCAGAGCAGATATGATTGATTTGAAAACTCTAGATATGCAGCTAGAGAAGCACTTGAGCCGTGTCTGGTCAAAGAGCATTGAGAATCAAAGGCCTAAGGAGGTGTGGGAGATTGATTTGTCTAAACTTGACATCATAAAGGAAGTTGCTCAAGGGACTTACGGAACAGTATACCAGGGTACATATGACAATCAAGTTGTTGCAGGTAAACATCCTTTATGCTTCATCTTTTCGCTTTTGGTTTTACTTCTGCTTAGATTCGGAGATGTGAAGGAACTTATTGGAAAGTGGCTGTCATTGATACTTTTTTTTATTGATGCCAAAACATGGTTTCTATATTTTCATACACAAGAATTACTGAATGGACATTGGAAAATAATATGATAATCTTGGAATTCTATATGTATAATTTAAAGCTTTGGTGAATTTAGGAATTCAACGTGAGTTGTTTAGAGTTTAGTTTGATAATAGATCTTAACGTGAAACATATTCTTCTTTATCCATGAAAAGTTGAAAAATTCTTTCTGAGCCTTAAAATCATGAAGCCTCTTTTTAATGAGTTGCCCACTTGTGGGGAACGATGTCAAGGTATGGAGTCAGCTTCAAGCTGGGTGTTTGTATGACCTTTTACATTGTTGCTTTATGAGGGGGGAATACTTTAATTCTGAATGCTGTTACTTTTTATTTACGAACACATTGGCCTAAGGGTTTTCGTTGATATCAAATTGTAGCTGAACTGAACTTTTCTATATTACAGTGAAGGTATTGGACTGGGGGGAGGAGGGTCTAGCCACAACGGCTGAAACCGCTGCTTTGCGGGCATCATTTCGGCAAGAGGTTGCTGTTTGGCACAAGCTTGACCATCCGAATGTTACAAAAGTACAAACTTCTTCCTCAAGTTCCTATAGTGAATGGTTGATGTTCATGGGAACATTGAAATATACGACAATTTTTTGGCTATATTTTTCTGTATATGAATTTTGCCTTTTCTTTTACTATGGTTTTGAGGTGGGTAATGATTATCCTTTTTTTATCTTTTATAACAGTTTGTCGGAGCCTCAATGGGAGCTACAAATCTTAAAATTCCAACGGATGATCAATATTCGTTTCCATCTAGGGCCTGTTGTGTTGTTGTTGAGTTTGTTTCTAGTGGGACATTAAAACAACACTTGATTAGATACTGTAGGAAGAAACTTTGCTTTAGGGATGTGATGAAATTAGCTTTGGATCTCTCTAGAGGGTAACTCTCTCTCTCTCTCTCTCTCTCTCTCTGTCAGCAATGCTTGACATCATCTTCTCTAAAGTAGAAGATTTGCTGATTAGGATTATAACTAATTTTACAGGCTTAGCTATCTACACTCCAAGAAGATTGTGCATCGTGATGTCAAAACTGAAAATATGTTGATGTCGGGTGACAACACTGTCAAAATTGCTGATTTTGGGGTTGCCCGTGTTGAAGCTCAGAATCCAAGAGATATGACTGGGGAAACTGGTACTCTTGGATACATGGCCCCAGAGGTAACTTTCTCATTCTGTTCTTAATACACGTGATCGTTCGGCATAGCTATGTTGTTTTTATCGTGTAATAGCAATTAGAACCGCATGTAGATATTCACCAATTAAAAATATATTTGGAAAAAAGGATACTGATTTGAAATATTATGTTCTGCTCCTCCTGCTTTTGTTTTTTCTTATCAACGTGGATTCTTTTGCATGAAACTTTTATCTCCTTGGAGGCCCAAATTCAATTTACCCGCTCTATCTGGATTTGCTTTCTTCTTGGTGGCTTGTTCTTTAACCTACCTAGAATGATTTTCAAAATAATTTTAGTGGTTGTGTTTGCTCAAACATGTTGTTCATTATGAATAATTATGGATGATAATCATATCTCATGCCTAGCTCTATAGTAGGCGTTAACATGCCAATATTCTATCACGCGTACAGGTTCTTGAAGGGAAGCCGTACAATAGAAGATGTGATGTCTATAGCTTTGGCATCTGCTTGTGGGAAATTTACTGCTGTGATATGCCATACGCTGATCTTAGTTTCGCTGACGTCTCATCTGCAGTTGTTCGACAGGTATGGTACTTTGGACCTAAGACATAAAACATGTCTCTTTAGGTCTCTTGTTCATTGCCATTATTCCTTATGTTTGTTGACACCAGTAGGAGTAGAGCTTGAAAATAATTTCTATTCAATACCCTCTTCCCATTTATTTAGTAGTGTTATGAATTCTTTGTTTATGCATATAGTCCATTAAGAAGTTTTGAAGCATCATTTTGTTTTTGCCAGAATCTGCGGCCCAGTATCCCGAGATGCTGTCCAAGTTCTCTGGCAAATGTGATGAAGAAATGCTGGGATGCCAATCCAGCCAAACGCCCTGAAATGCATGAGGTTGTCAAAATGTTGGAAGCCATTGATACAAGCAGAGGAGGGGGGATGATTTCTCCCGATCACGCTACCTGTTTCTGTTTCGGGCCTTTCCGCAAGATCCGTGGTCCATAATTCATTCATACTCTCATCCCTTGATGATGTAGAAATAAAGGAGAGTCAAAACACTATGCTGGTTTTTCCTTTTTCTTTTGCGCCCTGTTATTTGATGTTATAATATTTGCTTGTAACTGCTGAAATTTGCTCTCATATTGTCACTACTGCAGTTACCAATCTGTCTTTGTTTCAGAATTTTTCATTGAAGCGTTCTCTTCCCATTAGGTTTTTGATTTGTGCCTTTTAAGAATTTGGTGGTGGTTTGAATTTTATTATGGTTTACATTGTATATGAACGAATACAACCCATTTGGTTGGTTTACACAGTTGAGATTTTCTCTAACAAAATTTCCTATGTTATATTAATTTGTGATTATGGGT

mRNA sequence

CGTTGTTAAAACTCTATTTAGAATATTTGTGACAAGTCTCGTACTGGCATACAATTTTCTCTTACATTTTTTTAGTCATATTTACTTTAAATTACAAATTTATTATACATTCAATCAGGTTAAAAAGAAGGAGGAATCTTGGATCATCCATCCGTTGGATACATACATACAGGTAATTCAGTTGAAAAAGAATTTCATCTCCGGGCTCTGTGTTTATCTTTCGCTAATTTTAGTATAAGCACTTTCATTGAATTTGAAAGAGTCAGTATCGATTCCGAACCCTATGATATAGTGAAATCCCCGCGCTTTTCTCTTCGTCGTTCCCAAAATCCTGTGGTGTTACGGTTCCCATTGCTGGTTTCTTCTTTTTTAGAAAATTTGGTTTCGATTCTTCGTTGCCTTGCGATTCTCTGTTCTTCCATAACCCCTTTCGTGTCCTGTGGGTTTGCTGCAGCGAACCCATTGGACTAAAACAACTAGAATCGAAGTTTTTTTTCAATTCTCTGCTTTTCCATGATAGCGCCAATGGCGCTTCAGTGATTTTTTGTTGGCTTTGTATCTCTTTCTTCTACCTTTGAATCTTAAAAGCATAATACCCTACTTACCGCCTCTTTCATTATCATTCGCTGTCTTAGTCAGCATTTACGGTCTTCTTCTTTCTGGCGAGGGCTCCTTTAGGGTCTGCGGGTCCGATTTTGATGAGAGGTTGAAGGATTGTCTATGATAATGGGAATTATGACAGACAACAACATTCTGTTCAGTTATCACTTTACCGGGCTGCAATCTTCACCCTGTTTGATCTCTCAGGTCTAAAATTCCTTGTATTCAAGGTGTACTATTTGAGTTATCTGTGTTGATCCTAGCAATGGATTCGAAAGGAAATGATATGGGGGGTACTGCGATCCCAAAGGAGACACAGGGCCAGGATAGAACTCTGAACTCAAAGGTGGCTGGTATGGGAAGCATTAGCAGCAAAGATATGATATTCAGAGCAGATATGATTGATTTGAAAACTCTAGATATGCAGCTAGAGAAGCACTTGAGCCGTGTCTGGTCAAAGAGCATTGAGAATCAAAGGCCTAAGGAGGTGTGGGAGATTGATTTGTCTAAACTTGACATCATAAAGGAAGTTGCTCAAGGGACTTACGGAACAGTATACCAGGGTACATATGACAATCAAGTTGTTGCAGTGAAGGTATTGGACTGGGGGGAGGAGGGTCTAGCCACAACGGCTGAAACCGCTGCTTTGCGGGCATCATTTCGGCAAGAGTTTGTCGGAGCCTCAATGGGAGCTACAAATCTTAAAATTCCAACGGATGATCAATATTCGTTTCCATCTAGGGCCTGTTGTGTTGTTGTTGAGTTTGTTTCTAGTGGGACATTAAAACAACACTTGATTAGATACTGTAGGAAGAAACTTTGCTTTAGGGATGTGATGAAATTAGCTTTGGATCTCTCTAGAGGGCTTAGCTATCTACACTCCAAGAAGATTGTGCATCGTGATGTCAAAACTGAAAATATGTTGATGTCGGGTGACAACACTGTCAAAATTGCTGATTTTGGGGTTGCCCGTGTTGAAGCTCAGAATCCAAGAGATATGACTGGGGAAACTGGTACTCTTGGATACATGGCCCCAGAGGTTCTTGAAGGGAAGCCGTACAATAGAAGATGTGATGTCTATAGCTTTGGCATCTGCTTGTGGGAAATTTACTGCTGTGATATGCCATACGCTGATCTTAGTTTCGCTGACGTCTCATCTGCAGTTGTTCGACAGAATCTGCGGCCCAGTATCCCGAGATGCTGTCCAAGTTCTCTGGCAAATGTGATGAAGAAATGCTGGGATGCCAATCCAGCCAAACGCCCTGAAATGCATGAGGTTGTCAAAATGTTGGAAGCCATTGATACAAGCAGAGGAGGGGGGATGATTTCTCCCGATCACGCTACCTGTTTCTGTTTCGGGCCTTTCCGCAAGATCCGTGGTCCATAATTCATTCATACTCTCATCCCTTGATGATGTAGAAATAAAGGAGAGTCAAAACACTATGCTGGTTTTTCCTTTTTCTTTTGCGCCCTGTTATTTGATGTTATAATATTTGCTTGTAACTGCTGAAATTTGCTCTCATATTGTCACTACTGCAGTTACCAATCTGTCTTTGTTTCAGAATTTTTCATTGAAGCGTTCTCTTCCCATTAGGTTTTTGATTTGTGCCTTTTAAGAATTTGGTGGTGGTTTGAATTTTATTATGGTTTACATTGTATATGAACGAATACAACCCATTTGGTTGGTTTACACAGTTGAGATTTTCTCTAACAAAATTTCCTATGTTATATTAATTTGTGATTATGGGT

Coding sequence (CDS)

ATGGATTCGAAAGGAAATGATATGGGGGGTACTGCGATCCCAAAGGAGACACAGGGCCAGGATAGAACTCTGAACTCAAAGGTGGCTGGTATGGGAAGCATTAGCAGCAAAGATATGATATTCAGAGCAGATATGATTGATTTGAAAACTCTAGATATGCAGCTAGAGAAGCACTTGAGCCGTGTCTGGTCAAAGAGCATTGAGAATCAAAGGCCTAAGGAGGTGTGGGAGATTGATTTGTCTAAACTTGACATCATAAAGGAAGTTGCTCAAGGGACTTACGGAACAGTATACCAGGGTACATATGACAATCAAGTTGTTGCAGTGAAGGTATTGGACTGGGGGGAGGAGGGTCTAGCCACAACGGCTGAAACCGCTGCTTTGCGGGCATCATTTCGGCAAGAGTTTGTCGGAGCCTCAATGGGAGCTACAAATCTTAAAATTCCAACGGATGATCAATATTCGTTTCCATCTAGGGCCTGTTGTGTTGTTGTTGAGTTTGTTTCTAGTGGGACATTAAAACAACACTTGATTAGATACTGTAGGAAGAAACTTTGCTTTAGGGATGTGATGAAATTAGCTTTGGATCTCTCTAGAGGGCTTAGCTATCTACACTCCAAGAAGATTGTGCATCGTGATGTCAAAACTGAAAATATGTTGATGTCGGGTGACAACACTGTCAAAATTGCTGATTTTGGGGTTGCCCGTGTTGAAGCTCAGAATCCAAGAGATATGACTGGGGAAACTGGTACTCTTGGATACATGGCCCCAGAGGTTCTTGAAGGGAAGCCGTACAATAGAAGATGTGATGTCTATAGCTTTGGCATCTGCTTGTGGGAAATTTACTGCTGTGATATGCCATACGCTGATCTTAGTTTCGCTGACGTCTCATCTGCAGTTGTTCGACAGAATCTGCGGCCCAGTATCCCGAGATGCTGTCCAAGTTCTCTGGCAAATGTGATGAAGAAATGCTGGGATGCCAATCCAGCCAAACGCCCTGAAATGCATGAGGTTGTCAAAATGTTGGAAGCCATTGATACAAGCAGAGGAGGGGGGATGATTTCTCCCGATCACGCTACCTGTTTCTGTTTCGGGCCTTTCCGCAAGATCCGTGGTCCATAA

Protein sequence

MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLSRVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLATTAETAALRASFRQEFVGASMGATNLKIPTDDQYSFPSRACCVVVEFVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNTVKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDMPYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAIDTSRGGGMISPDHATCFCFGPFRKIRGP
Homology
BLAST of Lsi05G016260 vs. ExPASy Swiss-Prot
Match: Q9ZQ31 (Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 PE=1 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 8.9e-55
Identity = 112/275 (40.73%), Postives = 176/275 (64.00%), Query Frame = 0

Query: 76  WEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLATTAETAALRASFRQE 135
           W IDL KL++    AQG +G +Y+GTY+ + VA+K+L+  E    +  +   +   F+QE
Sbjct: 123 WTIDLRKLNMGPAFAQGAFGKLYKGTYNGEDVAIKILERPEN---SPEKAQFMEQQFQQE 182

Query: 136 FVGASMGATNLKIPTDDQYSFPSR---ACCVVVEFVSSGTLKQHLIRYCRKKLCFRDVMK 195
               SM A NLK P   ++    R     C+V E+   G+++Q L R   + +  +  +K
Sbjct: 183 ---VSMLA-NLKHPNIVRFIGACRKPMVWCIVTEYAKGGSVRQFLTRRQNRAVPLKLAVK 242

Query: 196 LALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNTVKIADFGVARVEAQNPRDMTGETGTL 255
            ALD++RG++Y+H +  +HRD+K++N+L+S D ++KIADFGVAR+E Q    MT ETGT 
Sbjct: 243 QALDVARGMAYVHGRNFIHRDLKSDNLLISADKSIKIADFGVARIEVQT-EGMTPETGTY 302

Query: 256 GYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDMPYADLSFADVSSAVVRQNLRPSIPRC 315
            +MAPE+++ + YN++ DVYSFGI LWE+    +P+ +++    + AVV + +RP++P  
Sbjct: 303 RWMAPEMIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAVQAAFAVVNRGVRPTVPND 362

Query: 316 CPSSLANVMKKCWDANPAKRPEMHEVVKMLEAIDT 348
           C   L+++M +CWDANP  RP   EVVK+LEA +T
Sbjct: 363 CLPVLSDIMTRCWDANPEVRPCFVEVVKLLEAAET 389

BLAST of Lsi05G016260 vs. ExPASy Swiss-Prot
Match: Q2MHE4 (Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=HT1 PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.0e-50
Identity = 110/316 (34.81%), Postives = 176/316 (55.70%), Query Frame = 0

Query: 48  LKTLDMQLEKHLSRVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVV 107
           +++  M LE      W  S   +  +E W  DLS+L I  + A G +  +Y+G Y  + V
Sbjct: 54  MESWSMILESENVETWEAS---KGEREEWTADLSQLFIGNKFASGAHSRIYRGIYKQRAV 113

Query: 108 AVKVLDWGEEGLATTAETAALRASFRQEFVGASMGATNLKIPTDDQYSFPSR---ACCVV 167
           AVK++      + T  E    RA   Q+F       + L  P   Q+    +     C++
Sbjct: 114 AVKMV-----RIPTHKEET--RAKLEQQFKSEVALLSRLFHPNIVQFIAACKKPPVYCII 173

Query: 168 VEFVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGD 227
            E++S G L+ +L +     L    V++LALD+SRG+ YLHS+ ++HRD+K+ N+L++ +
Sbjct: 174 TEYMSQGNLRMYLNKKEPYSLSIETVLRLALDISRGMEYLHSQGVIHRDLKSNNLLLNDE 233

Query: 228 NTVKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCC 287
             VK+ADFG + +E Q  R+  G  GT  +MAPE+++ KPY R+ DVYSFGI LWE+   
Sbjct: 234 MRVKVADFGTSCLETQ-CREAKGNMGTYRWMAPEMIKEKPYTRKVDVYSFGIVLWELTTA 293

Query: 288 DMPYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEA 347
            +P+  ++    + AV  +N RP +P  C  +LA+++K+CW  NP+KRP+   +V +LE 
Sbjct: 294 LLPFQGMTPVQAAFAVAEKNERPPLPASCQPALAHLIKRCWSENPSKRPDFSNIVAVLEK 353

Query: 348 IDTSRGGGMISPDHAT 361
            D     G+    HA+
Sbjct: 354 YDECVKEGLPLTSHAS 358

BLAST of Lsi05G016260 vs. ExPASy Swiss-Prot
Match: Q8RWL6 (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.5e-49
Identity = 106/277 (38.27%), Postives = 167/277 (60.29%), Query Frame = 0

Query: 76  WEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLD---WGEEGLATTAETAALRASF 135
           WEID+ +L I K+VA G+YG +++GTY +Q VA+K+L       E L   ++   +    
Sbjct: 285 WEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILKPERVNAEMLREFSQEVYIMRKV 344

Query: 136 RQ----EFVGASMGATNLKIPTDDQYSFPSRACCVVVEFVSSGTLKQHLIRYCRKKLCFR 195
           R     +F+GA   + NL               C+V EF++ G++   L ++ +     +
Sbjct: 345 RHKNVVQFIGACTRSPNL---------------CIVTEFMTRGSIYDFLHKH-KGVFKIQ 404

Query: 196 DVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNTVKIADFGVARVEAQNPRDMTGE 255
            ++K+ALD+S+G++YLH   I+HRD+KT N+LM     VK+ADFGVARV+ ++   MT E
Sbjct: 405 SLLKVALDVSKGMNYLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTES-GVMTAE 464

Query: 256 TGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDMPYADLSFADVSSAVVRQNLRPS 315
           TGT  +MAPEV+E KPY+ R DV+S+ I LWE+   ++PY+ L+    +  VV++ LRP 
Sbjct: 465 TGTYRWMAPEVIEHKPYDHRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPK 524

Query: 316 IPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAI 346
           IP+     L  +++KCW  +PA RP   E+++ML  +
Sbjct: 525 IPKETHPKLTELLEKCWQQDPALRPNFAEIIEMLNQL 544

BLAST of Lsi05G016260 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 1.5e-49
Identity = 109/274 (39.78%), Postives = 169/274 (61.68%), Query Frame = 0

Query: 76  WEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLD---WGEEGLATTAETAALRASF 135
           WEID+++L I K+VA G+YG +++GTY +Q VA+K L       E L   ++   +    
Sbjct: 279 WEIDVTQLKIEKKVASGSYGDLHRGTYCSQEVAIKFLKPDRVNNEMLREFSQEVFIMRKV 338

Query: 136 RQEFVGASMGATNLKIPTDDQYSFPSRACCVVVEFVSSGTLKQHLIRY-CRKKLCFRDVM 195
           R + V   +GA   + PT           C+V EF++ G++   L +  C  KL  + ++
Sbjct: 339 RHKNVVQFLGACT-RSPT----------LCIVTEFMARGSIYDFLHKQKCAFKL--QTLL 398

Query: 196 KLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNTVKIADFGVARVEAQNPRDMTGETGT 255
           K+ALD+++G+SYLH   I+HRD+KT N+LM     VK+ADFGVARV+ ++   MT ETGT
Sbjct: 399 KVALDVAKGMSYLHQNNIIHRDLKTANLLMDEHGLVKVADFGVARVQIES-GVMTAETGT 458

Query: 256 LGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDMPYADLSFADVSSAVVRQNLRPSIPR 315
             +MAPEV+E KPYN + DV+S+ I LWE+   D+PYA L+    +  VV++ LRP IP+
Sbjct: 459 YRWMAPEVIEHKPYNHKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPK 518

Query: 316 CCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAI 346
                +  ++++CW  +P +RP   E+++ML+ I
Sbjct: 519 KTHPKVKGLLERCWHQDPEQRPLFEEIIEMLQQI 538

BLAST of Lsi05G016260 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.3e-45
Identity = 103/281 (36.65%), Postives = 164/281 (58.36%), Query Frame = 0

Query: 74  EVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVL-------DWGEEGLATTAETA 133
           +VWEI+L  L    ++A G+YG +Y+GTY +Q VA+KVL       D  +E         
Sbjct: 281 DVWEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLKPERLDSDLEKEFAQEVFIMR 340

Query: 134 ALRASFRQEFVGASMGATNLKIPTDDQYSFPSRACCVVVEFVSSGTLKQHLIRYCRKKLC 193
            +R     +F+GA     +L               C+V EF+  G++  +L    ++K  
Sbjct: 341 KVRHKNVVQFIGACTKPPHL---------------CIVTEFMPGGSVYDYL---HKQKGV 400

Query: 194 FR--DVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNTVKIADFGVARVEAQNPRD 253
           F+   + K+A+D+ +G+SYLH   I+HRD+K  N+LM  +  VK+ADFGVARV+AQ    
Sbjct: 401 FKLPTLFKVAIDICKGMSYLHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQT-GV 460

Query: 254 MTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDMPYADLSFADVSSAVVRQN 313
           MT ETGT  +MAPEV+E KPY+ + DV+S+GI LWE+    +PY  ++    +  VV++ 
Sbjct: 461 MTAETGTYRWMAPEVIEHKPYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKG 520

Query: 314 LRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAI 346
           LRP+IP+     LA ++++ W+ +  +RP+  E+++ L+ I
Sbjct: 521 LRPTIPKNTHPKLAELLERLWEHDSTQRPDFSEIIEQLQEI 542

BLAST of Lsi05G016260 vs. ExPASy TrEMBL
Match: A0A0A0L4Q2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G146410 PE=4 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 6.2e-184
Identity = 323/386 (83.68%), Postives = 341/386 (88.34%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           MDSKGN MGG AIPKETQ QDRT NSKVAGMGSISSKDMIFRADMIDLKTLD+QLEKHLS
Sbjct: 1   MDSKGNVMGGAAIPKETQNQDRTPNSKVAGMGSISSKDMIFRADMIDLKTLDIQLEKHLS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWSKSI+NQ PKE WEIDLSKLD+IK++AQGTYGTVY+G YDNQ VAVK+LDWGEEGLA
Sbjct: 61  RVWSKSIDNQMPKEPWEIDLSKLDMIKQIAQGTYGTVYRGKYDNQEVAVKILDWGEEGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIPTDDQYSFPSRACCVVVE 180
           T AETAALRASFRQE              F+GASMGATNLKIP D Q SFPSRACCVVVE
Sbjct: 121 TMAETAALRASFRQEVAVWHKLDHPNVTKFIGASMGATNLKIPMDGQNSFPSRACCVVVE 180

Query: 181 FVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNT 240
           +V SGTLK HLIRY  KKL  + V+KLALDLSRGLSYLHSKKIVHRDVKTENMLM  ++ 
Sbjct: 181 YVPSGTLKDHLIRYWTKKLAIKAVVKLALDLSRGLSYLHSKKIVHRDVKTENMLMDINDN 240

Query: 241 VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM 300
           VKIADFGVARVEAQNPRDMTG TGTLGYMAPEVL+GKPYNR CDVYSFGICLWEIYCCDM
Sbjct: 241 VKIADFGVARVEAQNPRDMTGATGTLGYMAPEVLQGKPYNRSCDVYSFGICLWEIYCCDM 300

Query: 301 PYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID 360
           PYADLSFADVSSAVVR NLRPSIPRCCPSSLANVMKKCWDANP KRPEMHEVV+MLEAID
Sbjct: 301 PYADLSFADVSSAVVRHNLRPSIPRCCPSSLANVMKKCWDANPEKRPEMHEVVRMLEAID 360

Query: 361 TSRGGGMISPDHATCFCFGPFRKIRG 373
           TS+GGGMI+PD   CFC GPF K+RG
Sbjct: 361 TSKGGGMINPDDIKCFCLGPFGKLRG 386

BLAST of Lsi05G016260 vs. ExPASy TrEMBL
Match: A0A1S3AWZ2 (serine/threonine-protein kinase STY8-like OS=Cucumis melo OX=3656 GN=LOC103483687 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 322/386 (83.42%), Postives = 337/386 (87.31%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           MDSKGN MGG A+PKETQ QDRT NSKVAG GSISSKDMIFRAD IDLKTLD+QLEKHLS
Sbjct: 1   MDSKGNVMGGAAVPKETQNQDRTPNSKVAGTGSISSKDMIFRADKIDLKTLDIQLEKHLS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWSKSIENQ PKE WEIDLSKLDIIK++AQGTYGTVY+G YD+Q VAVK+LDWGEEGLA
Sbjct: 61  RVWSKSIENQMPKEPWEIDLSKLDIIKQIAQGTYGTVYRGKYDDQEVAVKILDWGEEGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIPTDDQYSFPSRACCVVVE 180
           T AETAALRASFRQE              F+GASMGATNLKI  D Q SFPSRACCVVVE
Sbjct: 121 TMAETAALRASFRQEVAVWHKLDHPNVTKFIGASMGATNLKISMDGQNSFPSRACCVVVE 180

Query: 181 FVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNT 240
           +V SGTLK HLIRY  KKLC + V+KLALDLSRGLSYLHSKKIVHRDVKTENMLM   + 
Sbjct: 181 YVPSGTLKDHLIRYWTKKLCIKAVVKLALDLSRGLSYLHSKKIVHRDVKTENMLMDIHHN 240

Query: 241 VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM 300
           VKIADFGVARVEAQNPRDMTG TGTLGYMAPEVL+GKPYNR CDVYSFGICLWEIYCCDM
Sbjct: 241 VKIADFGVARVEAQNPRDMTGATGTLGYMAPEVLQGKPYNRSCDVYSFGICLWEIYCCDM 300

Query: 301 PYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID 360
           PYADLSFADVSSAVVR NLRPSIPRCCPSSLANVMKKCWDANP KRPEMHEVVKMLEAID
Sbjct: 301 PYADLSFADVSSAVVRHNLRPSIPRCCPSSLANVMKKCWDANPEKRPEMHEVVKMLEAID 360

Query: 361 TSRGGGMISPDHATCFCFGPFRKIRG 373
           TS+GGGMI+PD   CFCF PF K RG
Sbjct: 361 TSKGGGMINPDDIKCFCFRPFGKFRG 386

BLAST of Lsi05G016260 vs. ExPASy TrEMBL
Match: A0A5D3CZT2 (Serine/threonine-protein kinase STY8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00660 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 322/386 (83.42%), Postives = 337/386 (87.31%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           MDSKGN MGG A+PKETQ QDRT NSKVAG GSISSKDMIFRAD IDLKTLD+QLEKHLS
Sbjct: 1   MDSKGNVMGGAAVPKETQNQDRTPNSKVAGTGSISSKDMIFRADKIDLKTLDIQLEKHLS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWSKSIENQ PKE WEIDLSKLDIIK++AQGTYGTVY+G YD+Q VAVK+LDWGEEGLA
Sbjct: 61  RVWSKSIENQMPKEPWEIDLSKLDIIKQIAQGTYGTVYRGKYDDQEVAVKILDWGEEGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIPTDDQYSFPSRACCVVVE 180
           T AETAALRASFRQE              F+GASMGATNLKI  D Q SFPSRACCVVVE
Sbjct: 121 TMAETAALRASFRQEVAVWHKLDHPNVTKFIGASMGATNLKISMDGQNSFPSRACCVVVE 180

Query: 181 FVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNT 240
           +V SGTLK HLIRY  KKLC + V+KLALDLSRGLSYLHSKKIVHRDVKTENMLM   + 
Sbjct: 181 YVPSGTLKDHLIRYWTKKLCIKAVVKLALDLSRGLSYLHSKKIVHRDVKTENMLMDIHHN 240

Query: 241 VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM 300
           VKIADFGVARVEAQNPRDMTG TGTLGYMAPEVL+GKPYNR CDVYSFGICLWEIYCCDM
Sbjct: 241 VKIADFGVARVEAQNPRDMTGATGTLGYMAPEVLQGKPYNRSCDVYSFGICLWEIYCCDM 300

Query: 301 PYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID 360
           PYADLSFADVSSAVVR NLRPSIPRCCPSSLANVMKKCWDANP KRPEMHEVVKMLEAID
Sbjct: 301 PYADLSFADVSSAVVRHNLRPSIPRCCPSSLANVMKKCWDANPEKRPEMHEVVKMLEAID 360

Query: 361 TSRGGGMISPDHATCFCFGPFRKIRG 373
           TS+GGGMI+PD   CFCF PF K RG
Sbjct: 361 TSKGGGMINPDDIKCFCFRPFGKFRG 386

BLAST of Lsi05G016260 vs. ExPASy TrEMBL
Match: A0A6J1GUV2 (serine/threonine-protein kinase HT1-like OS=Cucurbita moschata OX=3662 GN=LOC111457682 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.3e-175
Identity = 311/389 (79.95%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           M SKGNDMGGT IPKETQ QDRT NSKVAGMGSISSKDM+FRADMIDLKTLDMQLEKH S
Sbjct: 1   MGSKGNDMGGTTIPKETQNQDRTPNSKVAGMGSISSKDMVFRADMIDLKTLDMQLEKHFS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWS S +NQRPKE WEIDLSKLDI K +A GTYGTVY+G YDN+ VAVK+LDWGE+GLA
Sbjct: 61  RVWSMS-QNQRPKEEWEIDLSKLDIQKVIAHGTYGTVYKGAYDNKKVAVKLLDWGEDGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIP-TDDQYSFPSRACCVVV 180
           TTAETAALRASFRQE              FVGASMGATNLKIP T+DQ S PSRACCVVV
Sbjct: 121 TTAETAALRASFRQEVAVWHKLEHPNVTKFVGASMGATNLKIPSTEDQNSLPSRACCVVV 180

Query: 181 EFVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDN 240
           E++  GTLKQ LIR+ ++KL F+DV++LALDLSRGLSYLHSKKIVHRDVKTENML+    
Sbjct: 181 EYIPCGTLKQLLIRHKKRKLPFKDVVQLALDLSRGLSYLHSKKIVHRDVKTENMLIGAHR 240

Query: 241 TVKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD 300
           ++KIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD
Sbjct: 241 SLKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD 300

Query: 301 MPYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAI 360
           MPY +LSF DVSSAVVRQNLRPSIP+CCP+SLANVM+KCWD+NP+KRPEMHEVVKMLEAI
Sbjct: 301 MPYPNLSFVDVSSAVVRQNLRPSIPKCCPNSLANVMRKCWDSNPSKRPEMHEVVKMLEAI 360

Query: 361 DTSRGGGMISPDH-ATCFCFGPFRKIRGP 374
           DTS+GGGMI+ ++   CFCF PF ++RGP
Sbjct: 361 DTSKGGGMITREYFFGCFCFRPFHRLRGP 388

BLAST of Lsi05G016260 vs. ExPASy TrEMBL
Match: A0A6J1IVL5 (serine/threonine-protein kinase HT1-like OS=Cucurbita maxima OX=3661 GN=LOC111479023 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 7.6e-174
Identity = 309/389 (79.43%), Postives = 340/389 (87.40%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           M SKG DMGGT IPKETQ QDRT NSKVAGMGSISSKDM+FRADMIDLKTLDMQLEKH S
Sbjct: 1   MGSKGKDMGGTTIPKETQKQDRTPNSKVAGMGSISSKDMVFRADMIDLKTLDMQLEKHFS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWS + +NQRPKE WEIDLSKLDI K +A GTYGTVY+G YDN+ VAVK+LDWGE+GLA
Sbjct: 61  RVWSMT-QNQRPKEEWEIDLSKLDIQKVIAHGTYGTVYKGAYDNKKVAVKLLDWGEDGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIP-TDDQYSFPSRACCVVV 180
           TTAETAALRASFRQE              FVGASMGATNLKIP T+DQ S PSRACCVVV
Sbjct: 121 TTAETAALRASFRQEVAVWHKLEHPNVTKFVGASMGATNLKIPTTEDQNSLPSRACCVVV 180

Query: 181 EFVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDN 240
           E++  GTLKQ LIR+ ++KL F+DV++LALDLSRGLSYLHSKKIVHRDVKTENML+    
Sbjct: 181 EYIPCGTLKQLLIRHKKRKLPFKDVVQLALDLSRGLSYLHSKKIVHRDVKTENMLIGAHR 240

Query: 241 TVKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD 300
           ++KIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD
Sbjct: 241 SLKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD 300

Query: 301 MPYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAI 360
           MPY +LSF DVSSAVVRQNLRPSIP+CCP+SLANVM+KCWD+NP+KRPEMHEVVKMLEAI
Sbjct: 301 MPYPNLSFVDVSSAVVRQNLRPSIPKCCPNSLANVMRKCWDSNPSKRPEMHEVVKMLEAI 360

Query: 361 DTSRGGGMISPDH-ATCFCFGPFRKIRGP 374
           DTS GGGMI+ ++   CFCF PF ++RGP
Sbjct: 361 DTSNGGGMITREYFFGCFCFRPFHRLRGP 388

BLAST of Lsi05G016260 vs. NCBI nr
Match: XP_038877950.1 (serine/threonine-protein kinase STY13-like [Benincasa hispida] >XP_038877951.1 serine/threonine-protein kinase STY13-like [Benincasa hispida] >XP_038877952.1 serine/threonine-protein kinase STY13-like [Benincasa hispida] >XP_038877954.1 serine/threonine-protein kinase STY13-like [Benincasa hispida] >XP_038877955.1 serine/threonine-protein kinase STY13-like [Benincasa hispida])

HSP 1 Score: 683.3 bits (1762), Expect = 1.1e-192
Identity = 340/387 (87.86%), Postives = 349/387 (90.18%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           MDSKGNDMGG  IPKETQGQDRT+NSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS
Sbjct: 1   MDSKGNDMGGITIPKETQGQDRTMNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWSKSIENQRPKE WEIDLSKL+I K VAQGTYGTVY+GTYDNQ VAVKVLDWGEEGLA
Sbjct: 61  RVWSKSIENQRPKEAWEIDLSKLEIEKVVAQGTYGTVYKGTYDNQDVAVKVLDWGEEGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIPTDDQYSFPSRACCVVVE 180
           T AETAALRASFRQE              FVGASMGATNLKIP D Q  FPSRACCVVVE
Sbjct: 121 TLAETAALRASFRQEVAVWHKLDHPNVTKFVGASMGATNLKIPMDGQNLFPSRACCVVVE 180

Query: 181 FVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNT 240
           FV  GTLK++LIR C+KKL FR V+KLALDLSRGLSYLHS KIVHRDVKTENMLM  D+ 
Sbjct: 181 FVPGGTLKEYLIRSCKKKLEFRKVIKLALDLSRGLSYLHSMKIVHRDVKTENMLMDSDDN 240

Query: 241 VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM 300
           VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM
Sbjct: 241 VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM 300

Query: 301 PYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID 360
           PYADLSFADVSSAVVR NLRP IPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID
Sbjct: 301 PYADLSFADVSSAVVRHNLRPGIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID 360

Query: 361 TSRGGGMISPDHATCFCFGPFRKIRGP 374
           TSRGGGMISPD + CFCFGPFRKIRGP
Sbjct: 361 TSRGGGMISPDSSRCFCFGPFRKIRGP 387

BLAST of Lsi05G016260 vs. NCBI nr
Match: XP_004134436.1 (serine/threonine-protein kinase STY13 [Cucumis sativus] >XP_011650960.1 serine/threonine-protein kinase STY13 [Cucumis sativus] >XP_011650961.1 serine/threonine-protein kinase STY13 [Cucumis sativus] >XP_011650962.1 serine/threonine-protein kinase STY13 [Cucumis sativus] >XP_011650963.1 serine/threonine-protein kinase STY13 [Cucumis sativus] >XP_031739348.1 serine/threonine-protein kinase STY13 [Cucumis sativus])

HSP 1 Score: 653.3 bits (1684), Expect = 1.3e-183
Identity = 323/386 (83.68%), Postives = 341/386 (88.34%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           MDSKGN MGG AIPKETQ QDRT NSKVAGMGSISSKDMIFRADMIDLKTLD+QLEKHLS
Sbjct: 1   MDSKGNVMGGAAIPKETQNQDRTPNSKVAGMGSISSKDMIFRADMIDLKTLDIQLEKHLS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWSKSI+NQ PKE WEIDLSKLD+IK++AQGTYGTVY+G YDNQ VAVK+LDWGEEGLA
Sbjct: 61  RVWSKSIDNQMPKEPWEIDLSKLDMIKQIAQGTYGTVYRGKYDNQEVAVKILDWGEEGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIPTDDQYSFPSRACCVVVE 180
           T AETAALRASFRQE              F+GASMGATNLKIP D Q SFPSRACCVVVE
Sbjct: 121 TMAETAALRASFRQEVAVWHKLDHPNVTKFIGASMGATNLKIPMDGQNSFPSRACCVVVE 180

Query: 181 FVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNT 240
           +V SGTLK HLIRY  KKL  + V+KLALDLSRGLSYLHSKKIVHRDVKTENMLM  ++ 
Sbjct: 181 YVPSGTLKDHLIRYWTKKLAIKAVVKLALDLSRGLSYLHSKKIVHRDVKTENMLMDINDN 240

Query: 241 VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM 300
           VKIADFGVARVEAQNPRDMTG TGTLGYMAPEVL+GKPYNR CDVYSFGICLWEIYCCDM
Sbjct: 241 VKIADFGVARVEAQNPRDMTGATGTLGYMAPEVLQGKPYNRSCDVYSFGICLWEIYCCDM 300

Query: 301 PYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID 360
           PYADLSFADVSSAVVR NLRPSIPRCCPSSLANVMKKCWDANP KRPEMHEVV+MLEAID
Sbjct: 301 PYADLSFADVSSAVVRHNLRPSIPRCCPSSLANVMKKCWDANPEKRPEMHEVVRMLEAID 360

Query: 361 TSRGGGMISPDHATCFCFGPFRKIRG 373
           TS+GGGMI+PD   CFC GPF K+RG
Sbjct: 361 TSKGGGMINPDDIKCFCLGPFGKLRG 386

BLAST of Lsi05G016260 vs. NCBI nr
Match: KAE8650348.1 (hypothetical protein Csa_010699 [Cucumis sativus])

HSP 1 Score: 653.3 bits (1684), Expect = 1.3e-183
Identity = 323/386 (83.68%), Postives = 341/386 (88.34%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           MDSKGN MGG AIPKETQ QDRT NSKVAGMGSISSKDMIFRADMIDLKTLD+QLEKHLS
Sbjct: 1   MDSKGNVMGGAAIPKETQNQDRTPNSKVAGMGSISSKDMIFRADMIDLKTLDIQLEKHLS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWSKSI+NQ PKE WEIDLSKLD+IK++AQGTYGTVY+G YDNQ VAVK+LDWGEEGLA
Sbjct: 61  RVWSKSIDNQMPKEPWEIDLSKLDMIKQIAQGTYGTVYRGKYDNQEVAVKILDWGEEGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIPTDDQYSFPSRACCVVVE 180
           T AETAALRASFRQE              F+GASMGATNLKIP D Q SFPSRACCVVVE
Sbjct: 121 TMAETAALRASFRQEVAVWHKLDHPNVTKFIGASMGATNLKIPMDGQNSFPSRACCVVVE 180

Query: 181 FVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNT 240
           +V SGTLK HLIRY  KKL  + V+KLALDLSRGLSYLHSKKIVHRDVKTENMLM  ++ 
Sbjct: 181 YVPSGTLKDHLIRYWTKKLAIKAVVKLALDLSRGLSYLHSKKIVHRDVKTENMLMDINDN 240

Query: 241 VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM 300
           VKIADFGVARVEAQNPRDMTG TGTLGYMAPEVL+GKPYNR CDVYSFGICLWEIYCCDM
Sbjct: 241 VKIADFGVARVEAQNPRDMTGATGTLGYMAPEVLQGKPYNRSCDVYSFGICLWEIYCCDM 300

Query: 301 PYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID 360
           PYADLSFADVSSAVVR NLRPSIPRCCPSSLANVMKKCWDANP KRPEMHEVV+MLEAID
Sbjct: 301 PYADLSFADVSSAVVRHNLRPSIPRCCPSSLANVMKKCWDANPEKRPEMHEVVRMLEAID 360

Query: 361 TSRGGGMISPDHATCFCFGPFRKIRG 373
           TS+GGGMI+PD   CFC GPF K+RG
Sbjct: 361 TSKGGGMINPDDIKCFCLGPFGKLRG 386

BLAST of Lsi05G016260 vs. NCBI nr
Match: XP_008438633.1 (PREDICTED: serine/threonine-protein kinase STY8-like [Cucumis melo] >XP_008438634.1 PREDICTED: serine/threonine-protein kinase STY8-like [Cucumis melo] >XP_008438636.1 PREDICTED: serine/threonine-protein kinase STY8-like [Cucumis melo] >XP_008438637.1 PREDICTED: serine/threonine-protein kinase STY8-like [Cucumis melo] >XP_008438638.1 PREDICTED: serine/threonine-protein kinase STY8-like [Cucumis melo] >KAA0049340.1 serine/threonine-protein kinase STY8-like [Cucumis melo var. makuwa] >TYK17217.1 serine/threonine-protein kinase STY8-like [Cucumis melo var. makuwa])

HSP 1 Score: 647.9 bits (1670), Expect = 5.4e-182
Identity = 322/386 (83.42%), Postives = 337/386 (87.31%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           MDSKGN MGG A+PKETQ QDRT NSKVAG GSISSKDMIFRAD IDLKTLD+QLEKHLS
Sbjct: 1   MDSKGNVMGGAAVPKETQNQDRTPNSKVAGTGSISSKDMIFRADKIDLKTLDIQLEKHLS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWSKSIENQ PKE WEIDLSKLDIIK++AQGTYGTVY+G YD+Q VAVK+LDWGEEGLA
Sbjct: 61  RVWSKSIENQMPKEPWEIDLSKLDIIKQIAQGTYGTVYRGKYDDQEVAVKILDWGEEGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIPTDDQYSFPSRACCVVVE 180
           T AETAALRASFRQE              F+GASMGATNLKI  D Q SFPSRACCVVVE
Sbjct: 121 TMAETAALRASFRQEVAVWHKLDHPNVTKFIGASMGATNLKISMDGQNSFPSRACCVVVE 180

Query: 181 FVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNT 240
           +V SGTLK HLIRY  KKLC + V+KLALDLSRGLSYLHSKKIVHRDVKTENMLM   + 
Sbjct: 181 YVPSGTLKDHLIRYWTKKLCIKAVVKLALDLSRGLSYLHSKKIVHRDVKTENMLMDIHHN 240

Query: 241 VKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDM 300
           VKIADFGVARVEAQNPRDMTG TGTLGYMAPEVL+GKPYNR CDVYSFGICLWEIYCCDM
Sbjct: 241 VKIADFGVARVEAQNPRDMTGATGTLGYMAPEVLQGKPYNRSCDVYSFGICLWEIYCCDM 300

Query: 301 PYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAID 360
           PYADLSFADVSSAVVR NLRPSIPRCCPSSLANVMKKCWDANP KRPEMHEVVKMLEAID
Sbjct: 301 PYADLSFADVSSAVVRHNLRPSIPRCCPSSLANVMKKCWDANPEKRPEMHEVVKMLEAID 360

Query: 361 TSRGGGMISPDHATCFCFGPFRKIRG 373
           TS+GGGMI+PD   CFCF PF K RG
Sbjct: 361 TSKGGGMINPDDIKCFCFRPFGKFRG 386

BLAST of Lsi05G016260 vs. NCBI nr
Match: XP_023527176.1 (serine/threonine-protein kinase HT1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.5 bits (1612), Expect = 2.8e-175
Identity = 311/389 (79.95%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 1   MDSKGNDMGGTAIPKETQGQDRTLNSKVAGMGSISSKDMIFRADMIDLKTLDMQLEKHLS 60
           M SKGNDMGGT IPKETQ QDRT NSKVAGMGSISSKDM+FRADMIDLKTLDMQLEKH S
Sbjct: 1   MGSKGNDMGGTTIPKETQNQDRTPNSKVAGMGSISSKDMVFRADMIDLKTLDMQLEKHFS 60

Query: 61  RVWSKSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLA 120
           RVWS + +NQRPKE WEIDLSKLDI K +A GTYGTVY+G YDN+ VAVK+LDWGE+GLA
Sbjct: 61  RVWSMT-QNQRPKEEWEIDLSKLDIQKVIAHGTYGTVYKGAYDNKKVAVKLLDWGEDGLA 120

Query: 121 TTAETAALRASFRQE--------------FVGASMGATNLKIP-TDDQYSFPSRACCVVV 180
           TTAETAALRASFRQE              FVGASMGATNLKIP T+DQ S PSRACCVVV
Sbjct: 121 TTAETAALRASFRQEVAVWHKLEHPNVTKFVGASMGATNLKIPSTEDQNSLPSRACCVVV 180

Query: 181 EFVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDN 240
           E++  GTLKQ LIR+ ++KL F+DV++LALDLSRGLSYLHSKKIVHRDVKTENML+    
Sbjct: 181 EYIPCGTLKQLLIRHKKRKLPFKDVVQLALDLSRGLSYLHSKKIVHRDVKTENMLIGAHR 240

Query: 241 TVKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD 300
           ++KIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD
Sbjct: 241 SLKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCD 300

Query: 301 MPYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAI 360
           MPY DLSF DVSSAVVRQNLRPSIP+CCP+SLANVM+KCWD+NP+KRPEMHEVVKMLEAI
Sbjct: 301 MPYPDLSFVDVSSAVVRQNLRPSIPKCCPNSLANVMRKCWDSNPSKRPEMHEVVKMLEAI 360

Query: 361 DTSRGGGMISPDH-ATCFCFGPFRKIRGP 374
           DTS+GGGMI+ ++   CFCF PF ++RGP
Sbjct: 361 DTSKGGGMITREYFFGCFCFRPFHRLRGP 388

BLAST of Lsi05G016260 vs. TAIR 10
Match: AT3G22750.1 (Protein kinase superfamily protein )

HSP 1 Score: 509.2 bits (1310), Expect = 2.8e-144
Identity = 251/351 (71.51%), Postives = 289/351 (82.34%), Query Frame = 0

Query: 32  GSISSKDMIFRADMIDLKTLDMQLEKHLSRVWSKSIE-NQRPKEVWEIDLSKLDIIKEVA 91
           G+ S KDMIFRAD IDLK LD+QLEKHLSRVWS+SIE + +PKE WEI+L+KL++   +A
Sbjct: 22  GNNSKKDMIFRADKIDLKNLDIQLEKHLSRVWSRSIEKHPKPKEEWEIELAKLEMRNVIA 81

Query: 92  QGTYGTVYQGTYDNQVVAVKVLDWGEEGLATTAETAALRASFRQE--------------F 151
           +G YG VY+G YD Q VAVKVLDWGE+G ATTAET+ALRASFRQE              F
Sbjct: 82  RGAYGIVYKGIYDGQDVAVKVLDWGEDGYATTAETSALRASFRQEVAVWHKLDHPNVTRF 141

Query: 152 VGASMGATNLKIPT--DDQYSFPSRACCVVVEFVSSGTLKQHLIRYCRKKLCFRDVMKLA 211
           VGASMG  NLKIP+  + + S P RACCVVVE++  GTLKQ+L R  RKKL F+ V++LA
Sbjct: 142 VGASMGTANLKIPSSAETENSLPQRACCVVVEYIPGGTLKQYLFRNRRKKLAFKVVVQLA 201

Query: 212 LDLSRGLSYLHSKKIVHRDVKTENMLMSGDNTVKIADFGVARVEAQNPRDMTGETGTLGY 271
           LDLSRGLSYLHS++IVHRDVKTENML+     +KIADFGVARVEAQNP+DMTGETGTLGY
Sbjct: 202 LDLSRGLSYLHSERIVHRDVKTENMLLDYQRNLKIADFGVARVEAQNPKDMTGETGTLGY 261

Query: 272 MAPEVLEGKPYNRRCDVYSFGICLWEIYCCDMPYADLSFADVSSAVVRQNLRPSIPRCCP 331
           MAPEVL+GKPYNRRCDVYSFGICLWEIYCCDMPY DLSFADVSSAVVRQNLRP IPRCCP
Sbjct: 262 MAPEVLDGKPYNRRCDVYSFGICLWEIYCCDMPYPDLSFADVSSAVVRQNLRPDIPRCCP 321

Query: 332 SSLANVMKKCWDANPAKRPEMHEVVKMLEAIDTSRGGGMISPDHAT-CFCF 365
           ++LA +MK+CW+ANP KRPEM EVV +LEA+DT++GGGMI  D    CFCF
Sbjct: 322 TALATIMKRCWEANPEKRPEMEEVVSLLEAVDTTKGGGMIPEDQRPGCFCF 372

BLAST of Lsi05G016260 vs. TAIR 10
Match: AT4G14780.1 (Protein kinase superfamily protein )

HSP 1 Score: 491.1 bits (1263), Expect = 7.8e-139
Identity = 243/354 (68.64%), Postives = 280/354 (79.10%), Query Frame = 0

Query: 32  GSISSKDMIFRADMIDLKTLDMQLEKHLSRVWSKSIE-NQRPKEVWEIDLSKLDIIKEVA 91
           G+ ++K+ IFRAD IDLK+LD QLEKHLSRVWS+++E N + KE WEIDL+KL+    +A
Sbjct: 9   GNNTTKEKIFRADKIDLKSLDRQLEKHLSRVWSRNLEVNPKAKEEWEIDLAKLETSNVIA 68

Query: 92  QGTYGTVYQGTYDNQVVAVKVLDWGEEGLATTAETAALRASFRQE--------------F 151
           +GTYGTVY+G YD Q VAVKVLDW ++G  TTA+TA  RA FRQE              F
Sbjct: 69  RGTYGTVYKGIYDGQDVAVKVLDWEDDGNETTAKTATNRALFRQEVTVWHKLNHPNVTKF 128

Query: 152 VGASMGATNLKI-PTDDQYSFPSRACCVVVEFVSSGTLKQHLIRYCRKKLCFRDVMKLAL 211
           VGASMG TNL I   D + S P +ACCVVVE++  GTLKQHLIR+  KKL F+ V+KLAL
Sbjct: 129 VGASMGTTNLNIRSADSKGSLPQQACCVVVEYLPGGTLKQHLIRHKSKKLAFKAVIKLAL 188

Query: 212 DLSRGLSYLHSKKIVHRDVKTENMLMSGDNTVKIADFGVARVEAQNPRDMTGETGTLGYM 271
           DL+RGLSYLHS+KIVHRDVKTENML+     +KIADFGVARVEA NP+DMTGETGTLGYM
Sbjct: 189 DLARGLSYLHSEKIVHRDVKTENMLLDAQKNLKIADFGVARVEALNPKDMTGETGTLGYM 248

Query: 272 APEVLEGKPYNRRCDVYSFGICLWEIYCCDMPYADLSFADVSSAVVRQNLRPSIPRCCPS 331
           APEV++GKPYNRRCDVYSFGICLWEIYCCDMPY DLSF DVSSAVV  NLRP IPRCCP+
Sbjct: 249 APEVIDGKPYNRRCDVYSFGICLWEIYCCDMPYPDLSFVDVSSAVVLHNLRPEIPRCCPT 308

Query: 332 SLANVMKKCWDANPAKRPEMHEVVKMLEAIDTSRGGGMISPDHAT-CFCFGPFR 369
           +LA +MK CWD NP KRPEM EVVKMLE +DTS+GGGMI  D +  CFCF P R
Sbjct: 309 ALAGIMKTCWDGNPQKRPEMKEVVKMLEGVDTSKGGGMIPEDQSRGCFCFAPAR 362

BLAST of Lsi05G016260 vs. TAIR 10
Match: AT3G63260.1 (Protein kinase superfamily protein )

HSP 1 Score: 449.9 bits (1156), Expect = 2.0e-126
Identity = 233/391 (59.59%), Postives = 282/391 (72.12%), Query Frame = 0

Query: 9   GGTAIPKETQGQDRTLNSKVAGMGSISS-KDMIFRADMIDLKTLDMQL-EKHLSRVWSKS 68
           GG    K  +    T + K+ G GS S+ ++  FRAD +D    D+ + +   S V + S
Sbjct: 4   GGGEADKSLEIGSGTADPKIGGTGSRSAGEERYFRADTLDFSKWDLHMGQTSTSSVLTNS 63

Query: 69  IENQRP---KEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLATTA 128
                P    + WEIDLSKLD+   +A GTYGTVY+G Y  Q VAVKVLDWGE+G AT A
Sbjct: 64  ASTSAPAPAMQEWEIDLSKLDMKHVLAHGTYGTVYRGVYAGQEVAVKVLDWGEDGYATPA 123

Query: 129 ETAALRASFRQE--------------FVGASMGATNLKIPTDDQY------SFPSRACCV 188
           ET ALRASF QE              F+GASMG ++L+IP           + P+RACCV
Sbjct: 124 ETTALRASFEQEVAVWQKLDHPNVTKFIGASMGTSDLRIPPAGDTGGRGNGAHPARACCV 183

Query: 189 VVEFVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSG 248
           VVE+V+ GTLK+ LI+  R KL  +DV++LALDL+RGLSYLHSK IVHRDVK+ENML+  
Sbjct: 184 VVEYVAGGTLKKFLIKKYRAKLPIKDVIQLALDLARGLSYLHSKAIVHRDVKSENMLLQP 243

Query: 249 DNTVKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYC 308
           + T+KIADFGVARVEAQNP+DMTGETGTLGYMAPEVLEGKPYNR+CDVYSFG+CLWEIYC
Sbjct: 244 NKTLKIADFGVARVEAQNPQDMTGETGTLGYMAPEVLEGKPYNRKCDVYSFGVCLWEIYC 303

Query: 309 CDMPYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLE 368
           CDMPYAD SFA++S AVV +NLRP IP+CCP ++AN+MK+CWD NP +RPEM EVVK+LE
Sbjct: 304 CDMPYADCSFAEISHAVVHRNLRPEIPKCCPHAVANIMKRCWDPNPDRRPEMEEVVKLLE 363

Query: 369 AIDTSRGGGMISPDH-ATCFCFGPFRKIRGP 374
           AIDTS+GGGMI+PD    C C   F K RGP
Sbjct: 364 AIDTSKGGGMIAPDQFQGCLC---FFKPRGP 391

BLAST of Lsi05G016260 vs. TAIR 10
Match: AT3G01490.1 (Protein kinase superfamily protein )

HSP 1 Score: 439.5 bits (1129), Expect = 2.7e-123
Identity = 228/400 (57.00%), Postives = 271/400 (67.75%), Query Frame = 0

Query: 42  RADMIDLKTLDMQLEKHLSRVWS------------------------------------- 101
           RAD IDLK+LD QL++HLS+ W+                                     
Sbjct: 15  RADQIDLKSLDEQLQRHLSKAWTMEKRKSLSDGEDNVNNTRHNQNNFGHRQLVFQRPLLG 74

Query: 102 ---------------KSIENQRPKEVWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAV 161
                          +S E ++ +  WEID SKL I   +A+GT+GTV++G YD Q VAV
Sbjct: 75  GGYSNNNNSSKNDIIRSTEVEKSRREWEIDPSKLIIKSVIARGTFGTVHRGIYDGQDVAV 134

Query: 162 KVLDWGEEGLATTAETAALRASFRQE--------------FVGASMGATNLKIPTDD-QY 221
           K+LDWGEEG  + AE A+LRA+F QE              F+GA+MG + + I T++ Q 
Sbjct: 135 KLLDWGEEGHRSDAEIASLRAAFTQEVAVWHKLDHPNVTKFIGAAMGTSEMSIQTENGQM 194

Query: 222 SFPSRACCVVVEFVSSGTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDV 281
             PS  CCVVVE+   G LK  LI+  R+KL F+ V++L+LDL+RGLSYLHS+KIVHRDV
Sbjct: 195 GMPSNVCCVVVEYCPGGALKSFLIKTRRRKLAFKVVIQLSLDLARGLSYLHSQKIVHRDV 254

Query: 282 KTENMLMSGDNTVKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSF 341
           KTENML+    T+KIADFGVAR+EA NP DMTGETGTLGYMAPEVL G PYNR+CDVYSF
Sbjct: 255 KTENMLLDKSRTLKIADFGVARLEASNPNDMTGETGTLGYMAPEVLNGSPYNRKCDVYSF 314

Query: 342 GICLWEIYCCDMPYADLSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPE 374
           GICLWEIYCCDMPY DLSF++V+SAVVRQNLRP IPRCCPSSLANVMK+CWDANP KRPE
Sbjct: 315 GICLWEIYCCDMPYPDLSFSEVTSAVVRQNLRPEIPRCCPSSLANVMKRCWDANPEKRPE 374

BLAST of Lsi05G016260 vs. TAIR 10
Match: AT5G50000.1 (Protein kinase superfamily protein )

HSP 1 Score: 429.1 bits (1102), Expect = 3.6e-120
Identity = 224/384 (58.33%), Postives = 268/384 (69.79%), Query Frame = 0

Query: 37  KDMIFRADMIDLKTLDMQLEKHLSRVWSKSIENQRPKE---------------------- 96
           KD   RAD IDLK+LD QLE+HLSR  +     ++ +E                      
Sbjct: 5   KDGFVRADQIDLKSLDEQLERHLSRALTLEKNKKKDEEDTTAVAIGGSASSSPVTLNGGG 64

Query: 97  ---------VWEIDLSKLDIIKEVAQGTYGTVYQGTYDNQVVAVKVLDWGEEGLATTAET 156
                     WEID SKL I   +A+GT+GTV++G YD Q VAVK+LDWGEEG  + AE 
Sbjct: 65  FVGKRKQRLEWEIDPSKLIIKTVLARGTFGTVHRGIYDGQDVAVKLLDWGEEGHRSEAEI 124

Query: 157 AALRASFRQE--------------FVGASMGATNLKIPTDD-QYSFPSRACCVVVEFVSS 216
            +LRA F QE              F+GA+MGA+ L++ T+    + P+  CCVVVE++  
Sbjct: 125 VSLRADFAQEVAVWHKLDHPNVTKFIGATMGASGLQLQTESGPLAMPNNICCVVVEYLPG 184

Query: 217 GTLKQHLIRYCRKKLCFRDVMKLALDLSRGLSYLHSKKIVHRDVKTENMLMSGDNTVKIA 276
           G LK +LI+  R+KL F+ V++LALDL+RGLSYLHS+KIVHRDVKTENML+    TVKIA
Sbjct: 185 GALKSYLIKNRRRKLTFKIVVQLALDLARGLSYLHSQKIVHRDVKTENMLLDKTRTVKIA 244

Query: 277 DFGVARVEAQNPRDMTGETGTLGYMAPEVLEGKPYNRRCDVYSFGICLWEIYCCDMPYAD 336
           DFGVARVEA NP DMTGETGTLGYMAPEVL G PYNR+CDVYSFGICLWEIYCCDMPY D
Sbjct: 245 DFGVARVEASNPNDMTGETGTLGYMAPEVLNGNPYNRKCDVYSFGICLWEIYCCDMPYPD 304

Query: 337 LSFADVSSAVVRQNLRPSIPRCCPSSLANVMKKCWDANPAKRPEMHEVVKMLEAIDTSRG 374
           L+F++V+SAVVRQNLRP IPRCCPS+LA VMK+CWDANP KRPEM EVV MLE+IDT++G
Sbjct: 305 LTFSEVTSAVVRQNLRPDIPRCCPSALAAVMKRCWDANPDKRPEMDEVVPMLESIDTTKG 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZQ318.9e-5540.73Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 P... [more]
Q2MHE43.0e-5034.81Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8RWL61.5e-4938.27Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 P... [more]
O225581.5e-4939.78Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=... [more]
F4JTP51.3e-4536.65Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 P... [more]
Match NameE-valueIdentityDescription
A0A0A0L4Q26.2e-18483.68Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G146... [more]
A0A1S3AWZ22.6e-18283.42serine/threonine-protein kinase STY8-like OS=Cucumis melo OX=3656 GN=LOC10348368... [more]
A0A5D3CZT22.6e-18283.42Serine/threonine-protein kinase STY8-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1GUV22.3e-17579.95serine/threonine-protein kinase HT1-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1IVL57.6e-17479.43serine/threonine-protein kinase HT1-like OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
Match NameE-valueIdentityDescription
XP_038877950.11.1e-19287.86serine/threonine-protein kinase STY13-like [Benincasa hispida] >XP_038877951.1 s... [more]
XP_004134436.11.3e-18383.68serine/threonine-protein kinase STY13 [Cucumis sativus] >XP_011650960.1 serine/t... [more]
KAE8650348.11.3e-18383.68hypothetical protein Csa_010699 [Cucumis sativus][more]
XP_008438633.15.4e-18283.42PREDICTED: serine/threonine-protein kinase STY8-like [Cucumis melo] >XP_00843863... [more]
XP_023527176.12.8e-17579.95serine/threonine-protein kinase HT1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G22750.12.8e-14471.51Protein kinase superfamily protein [more]
AT4G14780.17.8e-13968.64Protein kinase superfamily protein [more]
AT3G63260.12.0e-12659.59Protein kinase superfamily protein [more]
AT3G01490.12.7e-12357.00Protein kinase superfamily protein [more]
AT5G50000.13.6e-12058.33Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 203..221
score: 42.43
coord: 313..335
score: 38.77
coord: 165..178
score: 33.48
coord: 269..291
score: 35.04
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 84..342
e-value: 6.0E-56
score: 189.6
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 83..345
e-value: 5.2E-50
score: 182.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 83..345
score: 36.866432
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 57..168
e-value: 1.7E-9
score: 39.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 169..352
e-value: 2.6E-59
score: 202.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR44329SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATEDcoord: 1..364
NoneNo IPR availablePANTHERPTHR44329:SF98KINASE ATMRK1, PUTATIVE-RELATEDcoord: 1..364
NoneNo IPR availableCDDcd13999STKc_MAP3K-likecoord: 89..342
e-value: 3.87392E-94
score: 279.423
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 209..221
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 62..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G016260.1Lsi05G016260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity