Lsi05G011390 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G011390
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	E3 ubiquitin-protein ligase CHIP
Location	chr05: 19146629 .. 19147729 (+)
RNA-Seq Expression	Lsi05G011390
Synteny	Lsi05G011390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTTCTCTGGTATTGTCCATTCTGTTTCCTTTTTTGATTTTAGTCAATTTACATGTATTGTTTCTCAGAGAAGCTTGTGAGGCTGCCCTTGAACAAAAATACTTTCTTGACCAATCTGAACTAGAAGGGTTTGTTGATGAAGCCAAAATTGCTCACCGGAAACAATTAAAATCGCTAAGAAGTGTGTTTGAAAAAGTTGCCGAGGCAGATGCGCCGTCCGAGGTAAGCATGTTGGTTACTACTTTTTTCAGTTTATTTCGTTGTTGTTACAAACTTTTAGGCATAACAGTTGCCATTTAAATCCTGTGGAGGGTACTAATTTGATATTTTCAGGTGCCTGATTATCTTTGCTGTAAAATCACACTCGATATTCTACGTGATCCCGTTATTACTCCAAGCGGGGTTACATATGAGAGAGCAGTGATTCTCGACCATTTTCGAAAGGTCGACATTTTCTCGCATCGCGATCCTACTACTACATGTCATGTAGCTACAATTCTGACTACTTTCCCTTTTGTCTAGGTTGGTAACTTCGATCCCATAACACGTGAGCTGCTCGACGAGTCCCAGCTAGTACCAAACTTAGCCATCAAGGAAGCAGTGCAATCATTTCTAGATAAGCATGGATGGGCTTATAATACAGATTAAAGAACTTTGTATTTTGAATGGGAACTCAATCAAGTTGATGGTTATGCCCTGTCAAGAATGAATGGAAATACTTCGACCCCATCTCGCTAATGTCGTAATTAACTCCTCGATTTGTCTGTATGGATCGTCGATTACGGTTTGTCGTAAGATATCATGCTTTCCATGACATTCTGAGGCTGTACTGTCAATTAAGTATGTGAGGTCCTGTCTTTTGATTTGCTCTTGTGCAGATTGTTACTATGAACTTGTTAATCTTCCTGTACAGTTATTACTTAAACCTTTGATTATGGCTTAGTTGTTGGAAGGCTCTGTTCAGCTGGCATTTCTTCCTTTCTAAAGTTTTGCCTTTAAAGTTGGATAAACTGCCCATTTCCATCATTTTTTTCTTTTCTATTTTTTTTTAAATACCATTTTGATCCCTCTTTTGGTTCGTGTAATTTAGATTTAGC

mRNA sequence

ATGGTCAATTTACATGTATTGTTTCTCAGAGAAGCTTGTGAGGCTGCCCTTGAACAAAAATACTTTCTTGACCAATCTGAACTAGAAGGGTTTGTTGATGAAGCCAAAATTGCTCACCGGAAACAATTAAAATCGCTAAGAAGTGTGTTTGAAAAAGTTGCCGAGGCAGATGCGCCGTCCGAGGTGCCTGATTATCTTTGCTGTAAAATCACACTCGATATTCTACGTGATCCCGTTATTACTCCAAGCGGGGTTACATATGAGAGAGCAGTGATTCTCGACCATTTTCGAAAGGTCGACATTTTCTCGCATCGCGATCCTACTACTACATGTCATGTTGGTAACTTCGATCCCATAACACGTGAGCTGCTCGACGAGTCCCAGCTAGTACCAAACTTAGCCATCAAGGAAGCAGTGCAATCATTTCTAGATAAGCATGGATGGGCTTATAATACAGATTAAAGAACTTTGTATTTTGAATGGGAACTCAATCAAGTTGATGGTTATGCCCTGTCAAGAATGAATGGAAATACTTCGACCCCATCTCGCTAATGTCGTAATTAACTCCTCGATTTGTCTGTATGGATCGTCGATTACGGTTTGTCGTAAGATATCATGCTTTCCATGACATTCTGAGGCTGTACTGTCAATTAAGTATGTGAGGTCCTGTCTTTTGATTTGCTCTTGTGCAGATTGTTACTATGAACTTGTTAATCTTCCTGTACAGTTATTACTTAAACCTTTGATTATGGCTTAGTTGTTGGAAGGCTCTGTTCAGCTGGCATTTCTTCCTTTCTAAAGTTTTGCCTTTAAAGTTGGATAAACTGCCCATTTCCATCATTTTTTTCTTTTCTATTTTTTTTTAAATACCATTTTGATCCCTCTTTTGGTTCGTGTAATTTAGATTTAGC

Coding sequence (CDS)

ATGGTCAATTTACATGTATTGTTTCTCAGAGAAGCTTGTGAGGCTGCCCTTGAACAAAAATACTTTCTTGACCAATCTGAACTAGAAGGGTTTGTTGATGAAGCCAAAATTGCTCACCGGAAACAATTAAAATCGCTAAGAAGTGTGTTTGAAAAAGTTGCCGAGGCAGATGCGCCGTCCGAGGTGCCTGATTATCTTTGCTGTAAAATCACACTCGATATTCTACGTGATCCCGTTATTACTCCAAGCGGGGTTACATATGAGAGAGCAGTGATTCTCGACCATTTTCGAAAGGTCGACATTTTCTCGCATCGCGATCCTACTACTACATGTCATGTTGGTAACTTCGATCCCATAACACGTGAGCTGCTCGACGAGTCCCAGCTAGTACCAAACTTAGCCATCAAGGAAGCAGTGCAATCATTTCTAGATAAGCATGGATGGGCTTATAATACAGATTAA

Protein sequence

MVNLHVLFLREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQLVPNLAIKEAVQSFLDKHGWAYNTD

Homology

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BLAST of Lsi05G011390 vs. ExPASy Swiss-Prot
Match: Q9SRS9 (E3 ubiquitin-protein ligase CHIP OS=Arabidopsis thaliana OX=3702 GN=CHIP PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 1.9e-40
Identity = 84/142 (59.15%), Postives = 100/142 (70.42%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+E CEAAL Q+  LD S  E   DEA  AH ++LK+L  VF+K AE D P+EVPDYLCC
Sbjct: 147 LKETCEAALNQQRALDMSRTEESSDEAYTAHTERLKALERVFKKAAEEDKPTEVPDYLCC 206

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
            ITL+I RDPVI+PSGVTYERA IL+H +K              VG FDPITRE +D + 
Sbjct: 207 NITLEIFRDPVISPSGVTYERAAILEHLKK--------------VGKFDPITREKIDPAN 266

Query: 129 LVPNLAIKEAVQSFLDKHGWAY 151
           LVPNLAIKEAV ++L+KH WAY
Sbjct: 267 LVPNLAIKEAVAAYLEKHVWAY 274

[Close]

BLAST of Lsi05G011390 vs. ExPASy Swiss-Prot
Match: Q5ZHY5 (E3 ubiquitin-protein ligase CHIP OS=Gallus gallus OX=9031 GN=STUB1 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 5.0e-20
Identity = 47/131 (35.88%), Postives = 78/131 (59.54%), Query Frame = 0


Query: 18  EQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCCKITLDILRD 77
           +Q+  +D+S     +   +  H K L  +  +F +V E     ++PDYLC KI+ +++R+
Sbjct: 194 QQEENVDESRGRVQLAGIEAKHDKYLADMDELFSQVDEKRKKRDIPDYLCGKISFELMRE 253

Query: 78  PVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQLVPNLAIKE 137
           P ITPSG+TY+R  I +H ++              VG+FDP+TR  L + QL+PNLA+KE
Sbjct: 254 PCITPSGITYDRKDIEEHLQR--------------VGHFDPVTRSPLTQDQLIPNLAMKE 310

Query: 138 AVQSFLDKHGW 149
            + +F+ ++GW
Sbjct: 314 VIDAFISENGW 310

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BLAST of Lsi05G011390 vs. ExPASy Swiss-Prot
Match: Q9UNE7 (E3 ubiquitin-protein ligase CHIP OS=Homo sapiens OX=9606 GN=STUB1 PE=1 SV=2)

HSP 1 Score: 98.2 bits (243), Expect = 8.5e-20
Identity = 46/115 (40.00%), Postives = 72/115 (62.61%), Query Frame = 0


Query: 34  EAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVIL 93
           EAK  H K +  +  +F +V E     ++PDYLC KI+ +++R+P ITPSG+TY+R  I 
Sbjct: 201 EAK--HDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIE 260

Query: 94  DHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQLVPNLAIKEAVQSFLDKHGW 149
           +H ++              VG+FDP+TR  L + QL+PNLA+KE + +F+ ++GW
Sbjct: 261 EHLQR--------------VGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 299

[Close]

BLAST of Lsi05G011390 vs. ExPASy Swiss-Prot
Match: Q9WUD1 (E3 ubiquitin-protein ligase CHIP OS=Mus musculus OX=10090 GN=Stub1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 8.5e-20
Identity = 46/115 (40.00%), Postives = 72/115 (62.61%), Query Frame = 0


Query: 34  EAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVIL 93
           EAK  H K +  +  +F +V E     ++PDYLC KI+ +++R+P ITPSG+TY+R  I 
Sbjct: 202 EAK--HDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIE 261

Query: 94  DHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQLVPNLAIKEAVQSFLDKHGW 149
           +H ++              VG+FDP+TR  L + QL+PNLA+KE + +F+ ++GW
Sbjct: 262 EHLQR--------------VGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGW 300

[Close]

BLAST of Lsi05G011390 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 3.7e-07
Identity = 28/86 (32.56%), Postives = 49/86 (56.98%), Query Frame = 0


Query: 57  DAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNF 116
           D   E+P +  C I+LDI++DPVI  +G+TY+R  I     +  +FS +         N 
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESI-----EKWLFSGKK--------NS 61

Query: 117 DPITRELLDESQLVPNLAIKEAVQSF 143
            P+T++++ E+ L PN  ++  +QS+
Sbjct: 62  CPVTKQVITETDLTPNHTLRRLIQSW 74

[Close]

BLAST of Lsi05G011390 vs. ExPASy TrEMBL
Match: A0A5D3DR86 (E3 ubiquitin-protein ligase CHIP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1185G00240 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 8.4e-63
Identity = 125/145 (86.21%), Postives = 130/145 (89.66%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLDQSELEGFVDEA+IAHRKQLKSLRSVFEKVAEAD+PSEVPDYLCC
Sbjct: 867 LKEACEAALEQKYFLDQSELEGFVDEAEIAHRKQLKSLRSVFEKVAEADSPSEVPDYLCC 926

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHF K              VGNFDPITRELLDESQ
Sbjct: 927 KITLDILRDPVITPSGVTYERAVILDHFNK--------------VGNFDPITRELLDESQ 986

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           L+PNLAIKEAVQSFLDKHGWAYNT+
Sbjct: 987 LIPNLAIKEAVQSFLDKHGWAYNTE 997

[Close]

BLAST of Lsi05G011390 vs. ExPASy TrEMBL
Match: A0A5A7VKZ1 (E3 ubiquitin-protein ligase CHIP OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G00360 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 8.4e-63
Identity = 125/145 (86.21%), Postives = 130/145 (89.66%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLDQSELEGFVDEA+IAHRKQLKSLRSVFEKVAEAD+PSEVPDYLCC
Sbjct: 112 LKEACEAALEQKYFLDQSELEGFVDEAEIAHRKQLKSLRSVFEKVAEADSPSEVPDYLCC 171

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHF K              VGNFDPITRELLDESQ
Sbjct: 172 KITLDILRDPVITPSGVTYERAVILDHFNK--------------VGNFDPITRELLDESQ 231

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           L+PNLAIKEAVQSFLDKHGWAYNT+
Sbjct: 232 LIPNLAIKEAVQSFLDKHGWAYNTE 242

[Close]

BLAST of Lsi05G011390 vs. ExPASy TrEMBL
Match: A0A1S4E2T5 (E3 ubiquitin-protein ligase CHIP OS=Cucumis melo OX=3656 GN=LOC103499262 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 8.4e-63
Identity = 125/145 (86.21%), Postives = 130/145 (89.66%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLDQSELEGFVDEA+IAHRKQLKSLRSVFEKVAEAD+PSEVPDYLCC
Sbjct: 140 LKEACEAALEQKYFLDQSELEGFVDEAEIAHRKQLKSLRSVFEKVAEADSPSEVPDYLCC 199

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHF K              VGNFDPITRELLDESQ
Sbjct: 200 KITLDILRDPVITPSGVTYERAVILDHFNK--------------VGNFDPITRELLDESQ 259

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           L+PNLAIKEAVQSFLDKHGWAYNT+
Sbjct: 260 LIPNLAIKEAVQSFLDKHGWAYNTE 270

[Close]

BLAST of Lsi05G011390 vs. ExPASy TrEMBL
Match: A0A6J1K6X7 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita maxima OX=3661 GN=LOC111491225 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.5e-62
Identity = 124/145 (85.52%), Postives = 129/145 (88.97%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLD SELEGFVDEA+IAHRKQLKSLRSVFEKV EADAPSEVPDYLCC
Sbjct: 152 LKEACEAALEQKYFLDHSELEGFVDEAEIAHRKQLKSLRSVFEKVGEADAPSEVPDYLCC 211

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHFRK              VGNFDP+TRELLDESQ
Sbjct: 212 KITLDILRDPVITPSGVTYERAVILDHFRK--------------VGNFDPLTRELLDESQ 271

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           LVPNLAIKEAV++FLDKHGWAYNTD
Sbjct: 272 LVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of Lsi05G011390 vs. ExPASy TrEMBL
Match: A0A6J1EYU4 (E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111437457 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.5e-62
Identity = 124/145 (85.52%), Postives = 129/145 (88.97%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLD SELEGFVDEA+IAHRKQLKSLRSVFEKV EADAPSEVPDYLCC
Sbjct: 152 LKEACEAALEQKYFLDHSELEGFVDEAEIAHRKQLKSLRSVFEKVGEADAPSEVPDYLCC 211

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHFRK              VGNFDP+TRELLDESQ
Sbjct: 212 KITLDILRDPVITPSGVTYERAVILDHFRK--------------VGNFDPLTRELLDESQ 271

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           LVPNLAIKEAV++FLDKHGWAYNTD
Sbjct: 272 LVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of Lsi05G011390 vs. NCBI nr
Match: TYK26068.1 (E3 ubiquitin-protein ligase CHIP [Cucumis melo var. makuwa])

HSP 1 Score: 249.6 bits (636), Expect = 1.7e-62
Identity = 125/145 (86.21%), Postives = 130/145 (89.66%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLDQSELEGFVDEA+IAHRKQLKSLRSVFEKVAEAD+PSEVPDYLCC
Sbjct: 867 LKEACEAALEQKYFLDQSELEGFVDEAEIAHRKQLKSLRSVFEKVAEADSPSEVPDYLCC 926

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHF K              VGNFDPITRELLDESQ
Sbjct: 927 KITLDILRDPVITPSGVTYERAVILDHFNK--------------VGNFDPITRELLDESQ 986

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           L+PNLAIKEAVQSFLDKHGWAYNT+
Sbjct: 987 LIPNLAIKEAVQSFLDKHGWAYNTE 997

[Close]

BLAST of Lsi05G011390 vs. NCBI nr
Match: XP_016902537.1 (PREDICTED: E3 ubiquitin-protein ligase CHIP [Cucumis melo])

HSP 1 Score: 249.6 bits (636), Expect = 1.7e-62
Identity = 125/145 (86.21%), Postives = 130/145 (89.66%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLDQSELEGFVDEA+IAHRKQLKSLRSVFEKVAEAD+PSEVPDYLCC
Sbjct: 140 LKEACEAALEQKYFLDQSELEGFVDEAEIAHRKQLKSLRSVFEKVAEADSPSEVPDYLCC 199

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHF K              VGNFDPITRELLDESQ
Sbjct: 200 KITLDILRDPVITPSGVTYERAVILDHFNK--------------VGNFDPITRELLDESQ 259

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           L+PNLAIKEAVQSFLDKHGWAYNT+
Sbjct: 260 LIPNLAIKEAVQSFLDKHGWAYNTE 270

[Close]

BLAST of Lsi05G011390 vs. NCBI nr
Match: KAA0068084.1 (E3 ubiquitin-protein ligase CHIP [Cucumis melo var. makuwa])

HSP 1 Score: 249.6 bits (636), Expect = 1.7e-62
Identity = 125/145 (86.21%), Postives = 130/145 (89.66%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLDQSELEGFVDEA+IAHRKQLKSLRSVFEKVAEAD+PSEVPDYLCC
Sbjct: 112 LKEACEAALEQKYFLDQSELEGFVDEAEIAHRKQLKSLRSVFEKVAEADSPSEVPDYLCC 171

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHF K              VGNFDPITRELLDESQ
Sbjct: 172 KITLDILRDPVITPSGVTYERAVILDHFNK--------------VGNFDPITRELLDESQ 231

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           L+PNLAIKEAVQSFLDKHGWAYNT+
Sbjct: 232 LIPNLAIKEAVQSFLDKHGWAYNTE 242

[Close]

BLAST of Lsi05G011390 vs. NCBI nr
Match: XP_022931210.1 (E3 ubiquitin-protein ligase CHIP-like [Cucurbita moschata] >XP_022995799.1 E3 ubiquitin-protein ligase CHIP-like [Cucurbita maxima])

HSP 1 Score: 248.1 bits (632), Expect = 5.1e-62
Identity = 124/145 (85.52%), Postives = 129/145 (88.97%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLD SELEGFVDEA+IAHRKQLKSLRSVFEKV EADAPSEVPDYLCC
Sbjct: 152 LKEACEAALEQKYFLDHSELEGFVDEAEIAHRKQLKSLRSVFEKVGEADAPSEVPDYLCC 211

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHFRK              VGNFDP+TRELLDESQ
Sbjct: 212 KITLDILRDPVITPSGVTYERAVILDHFRK--------------VGNFDPLTRELLDESQ 271

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNTD 154
           LVPNLAIKEAV++FLDKHGWAYNTD
Sbjct: 272 LVPNLAIKEAVEAFLDKHGWAYNTD 282

[Close]

BLAST of Lsi05G011390 vs. NCBI nr
Match: XP_038886937.1 (E3 ubiquitin-protein ligase CHIP isoform X1 [Benincasa hispida])

HSP 1 Score: 248.1 bits (632), Expect = 5.1e-62
Identity = 126/144 (87.50%), Postives = 128/144 (88.89%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+EACEAALEQKYFLDQSELEGFVDEA+IAHR QLKSLRSVFEKVAEADAPSEVPDYLCC
Sbjct: 151 LKEACEAALEQKYFLDQSELEGFVDEAEIAHRNQLKSLRSVFEKVAEADAPSEVPDYLCC 210

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
           KITLDILRDPVITPSGVTYERAVILDHF K              VGNFDPITRELLDESQ
Sbjct: 211 KITLDILRDPVITPSGVTYERAVILDHFHK--------------VGNFDPITRELLDESQ 270

Query: 129 LVPNLAIKEAVQSFLDKHGWAYNT 153
           LVPNLAIKEAVQSFLDKHGWAYNT
Sbjct: 271 LVPNLAIKEAVQSFLDKHGWAYNT 280

[Close]

BLAST of Lsi05G011390 vs. TAIR 10
Match: AT3G07370.1 (carboxyl terminus of HSC70-interacting protein )

HSP 1 Score: 166.8 bits (421), Expect = 1.4e-41
Identity = 84/142 (59.15%), Postives = 100/142 (70.42%), Query Frame = 0


Query: 9   LREACEAALEQKYFLDQSELEGFVDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCC 68
           L+E CEAAL Q+  LD S  E   DEA  AH ++LK+L  VF+K AE D P+EVPDYLCC
Sbjct: 147 LKETCEAALNQQRALDMSRTEESSDEAYTAHTERLKALERVFKKAAEEDKPTEVPDYLCC 206

Query: 69  KITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQ 128
            ITL+I RDPVI+PSGVTYERA IL+H +K              VG FDPITRE +D + 
Sbjct: 207 NITLEIFRDPVISPSGVTYERAAILEHLKK--------------VGKFDPITREKIDPAN 266

Query: 129 LVPNLAIKEAVQSFLDKHGWAY 151
           LVPNLAIKEAV ++L+KH WAY
Sbjct: 267 LVPNLAIKEAVAAYLEKHVWAY 274

[Close]

BLAST of Lsi05G011390 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 56.2 bits (134), Expect = 2.6e-08
Identity = 28/86 (32.56%), Postives = 49/86 (56.98%), Query Frame = 0


Query: 57  DAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNF 116
           D   E+P +  C I+LDI++DPVI  +G+TY+R  I     +  +FS +         N 
Sbjct: 2   DQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESI-----EKWLFSGKK--------NS 61

Query: 117 DPITRELLDESQLVPNLAIKEAVQSF 143
            P+T++++ E+ L PN  ++  +QS+
Sbjct: 62  CPVTKQVITETDLTPNHTLRRLIQSW 74

[Close]

BLAST of Lsi05G011390 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 54.3 bits (129), Expect = 1.0e-07
Identity = 30/93 (32.26%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 49  VFEKVAEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPT 108
           VF ++ + +   E+P+Y  C I+L+I++DPV T SG+TY+R  I+    KV         
Sbjct: 11  VFPQMDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKV--------- 70

Query: 109 TTCHVGNFDPITRELLD-ESQLVPNLAIKEAVQ 141
            +C      P+T++ L  +S L PN  ++  +Q
Sbjct: 71  PSC------PVTKQPLPLDSDLTPNHMLRRLIQ 88

[Close]

BLAST of Lsi05G011390 vs. TAIR 10
Match: AT2G45920.1 (U-box domain-containing protein )

HSP 1 Score: 53.5 bits (127), Expect = 1.7e-07
Identity = 29/94 (30.85%), Postives = 48/94 (51.06%), Query Frame = 0


Query: 51  EKVAEADAPSEVPDYLCCKITLDILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTT 110
           ++V+ +D   E P Y  C I+L++++DP +   G TYE   I               +T 
Sbjct: 314 KEVSRSDEHREAPQYFICPISLEVMKDPQLAADGFTYEAEAI---------------STW 373

Query: 111 CHVGN-FDPITRELLDESQLVPNLAIKEAVQSFL 144
              G+   P+T   L  ++LVPNLA++ A+Q +L
Sbjct: 374 LQGGHETSPMTNTKLHHTKLVPNLALRSAIQEWL 392

[Close]

BLAST of Lsi05G011390 vs. TAIR 10
Match: AT3G61390.2 (RING/U-box superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 5.0e-07
Identity = 39/130 (30.00%), Postives = 54/130 (41.54%), Query Frame = 0


Query: 17  LEQKYFLDQSELEGF---VDEAKIAHRKQLKSLRSVFEKVAEADAPSEVPDYLCCKITLD 76
           L++K  L      G     +E KI  R+  K      E+ A      E P Y  C IT D
Sbjct: 305 LQEKNNLSMETFRGIREEQEELKIKLREVSKLKGKREEEEASTSNHREPPQYFICPITHD 364

Query: 77  ILRDPVITPSGVTYERAVILDHFRKVDIFSHRDPTTTCHVGNFDPITRELLDESQLVPNL 136
           I+ DP +   G TYE   I   F +     H             P+  + L  + LVPNL
Sbjct: 365 IMEDPHVAADGFTYEGEAISRWFER----GHET----------SPMINKRLPHTSLVPNL 420

Query: 137 AIKEAVQSFL 144
           A++ A+Q +L
Sbjct: 425 ALRSAIQEWL 420

The following BLAST results are available for this feature:

BLAST of Lsi05G011390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRS9	1.9e-40	59.15	E3 ubiquitin-protein ligase CHIP OS=Arabidopsis thaliana OX=3702 GN=CHIP PE=1 SV...	[more]
Q5ZHY5	5.0e-20	35.88	E3 ubiquitin-protein ligase CHIP OS=Gallus gallus OX=9031 GN=STUB1 PE=2 SV=1	[more]
Q9UNE7	8.5e-20	40.00	E3 ubiquitin-protein ligase CHIP OS=Homo sapiens OX=9606 GN=STUB1 PE=1 SV=2	[more]
Q9WUD1	8.5e-20	40.00	E3 ubiquitin-protein ligase CHIP OS=Mus musculus OX=10090 GN=Stub1 PE=1 SV=1	[more]
Q9SVC6	3.7e-07	32.56	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]

BLAST of Lsi05G011390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DR86	8.4e-63	86.21	E3 ubiquitin-protein ligase CHIP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7VKZ1	8.4e-63	86.21	E3 ubiquitin-protein ligase CHIP OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S4E2T5	8.4e-63	86.21	E3 ubiquitin-protein ligase CHIP OS=Cucumis melo OX=3656 GN=LOC103499262 PE=4 SV...	[more]
A0A6J1K6X7	2.5e-62	85.52	E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita maxima OX=3661 GN=LOC11149122...	[more]
A0A6J1EYU4	2.5e-62	85.52	E3 ubiquitin-protein ligase CHIP-like OS=Cucurbita moschata OX=3662 GN=LOC111437...	[more]

BLAST of Lsi05G011390 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK26068.1	1.7e-62	86.21	E3 ubiquitin-protein ligase CHIP [Cucumis melo var. makuwa]	[more]
XP_016902537.1	1.7e-62	86.21	PREDICTED: E3 ubiquitin-protein ligase CHIP [Cucumis melo]	[more]
KAA0068084.1	1.7e-62	86.21	E3 ubiquitin-protein ligase CHIP [Cucumis melo var. makuwa]	[more]
XP_022931210.1	5.1e-62	85.52	E3 ubiquitin-protein ligase CHIP-like [Cucurbita moschata] >XP_022995799.1 E3 ub...	[more]
XP_038886937.1	5.1e-62	87.50	E3 ubiquitin-protein ligase CHIP isoform X1 [Benincasa hispida]	[more]

BLAST of Lsi05G011390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07370.1	1.4e-41	59.15	carboxyl terminus of HSC70-interacting protein	[more]
AT3G52450.1	2.6e-08	32.56	plant U-box 22	[more]
AT3G11840.1	1.0e-07	32.26	plant U-box 24	[more]
AT2G45920.1	1.7e-07	30.85	U-box domain-containing protein	[more]
AT3G61390.2	5.0e-07	30.00	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 65..142 e-value: 6.8E-19 score: 78.8
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 62..148 e-value: 2.9E-24 score: 85.0
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 61..149 score: 27.511892
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 60..152 e-value: 1.7E-23 score: 84.4
None	No IPR available	PANTHER	PTHR46803	E3 UBIQUITIN-PROTEIN LIGASE CHIP	coord: 9..151
None	No IPR available	CDD	cd16654	RING-Ubox_CHIP	coord: 62..142 e-value: 9.78961E-38 score: 121.583
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 57..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G011390.1	Lsi05G011390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071218	cellular response to misfolded protein
biological_process	GO:0045862	positive regulation of proteolysis
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0006515	protein quality control for misfolded or incompletely synthesized proteins
biological_process	GO:0009737	response to abscisic acid
biological_process	GO:0009651	response to salt stress
biological_process	GO:0009266	response to temperature stimulus
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity